pulsesequence()
{
/* DECLARE AND LOAD VARIABLES */
char f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
mag_flg[MAXSTR], /*magic angle gradient*/
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
codecseq[MAXSTR]; /* sequence for 13C' decoupling */
int icosel1, /* used to get n and p type */
icosel2,
t1_counter, /* used for states tppi in t1 */
ni2 = getval("ni2");
double tau1, /* t1 delay */
tau2, /* t2 delay */
del = getval("del"), /* time delays for CH coupling evolution */
del1 = getval("del1"),
del2 = getval("del2"),
del3 = getval("del3"),
del4 = getval("del4"),
TC = getval("TC"),
satpwr = getval("satpwr"),
waltzB1 = getval("waltzB1"),
spinlock = getval("spinlock"),
pwco,copwr, cores,codmf,
kappa,
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rf0, /* maximum fine power when using pwC pulses */
/* p_d is used to calculate the isotropic mixing on the Cab region */
p_d, /* 50 degree pulse for DIPSI-2 at rfd */
rfd, /* fine power for 7 kHz rf for 500MHz magnet */
ncyc = getval("ncyc"), /* no. of cycles of DIPSI-3 */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
pwHd, /* H1 90 degree pulse length at tpwrd */
tpwrd, /*rf for WALTZ decoupling */
sw1 = getval("sw1"),
sw2 = getval("sw2"),
gt1 = getval("gt1"), /* coherence pathway gradients */
gzcal = getval("gzcal"), /* G/cm to DAC coversion factor*/
gstab = getval("gstab"),
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gt3 = getval("gt3"), /* other gradients */
gt5 = getval("gt5"),
gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5"),
gzlvl6 = getval("gzlvl6");
getstr("mag_flg",mag_flg);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("codecseq",codecseq);
/* LOAD PHASE TABLE */
settable(t1,2,phi1);
settable(t2,1,phi2);
settable(t3,1,phi3);
settable(t4,1,phi4);
settable(t11,2,rec);
/* INITIALIZE VARIABLES */
if( dpwrf < 4095 )
{ printf("reset dpwrf=4095 and recalibrate C13 90 degree pulse");
psg_abort(1); }
/* maximum fine power for pwC pulses */
rf0 = 4095.0;
/* dipsi-3 decoupling on CbCa */
p_d = (5.0)/(9.0*4.0*spinlock); /* DIPSI-3*/
rfd = (compC*4095.0*pwC*5.0)/(p_d*9.0);
rfd = (int) (rfd + 0.5);
ncyc = (int) (ncyc + 0.5);
/* power level and pulse time for WALTZ 1H decoupling */
pwHd = 1/(4.0 * waltzB1) ;
tpwrd = tpwr - 20.0*log10(pwHd/(pw));
tpwrd = (int) (tpwrd + 0.5);
/* activate auto-calibration flags */
setautocal();
if (autocal[0] == 'n')
{
codmf= getval("codmf");
pwco = 1.0/codmf; /* pw for 13C' decoupling field */
copwr = getval("copwr"); /* power level for 13C' decoupling */
cores = getval("cores"); /* power level for 13C' decoupling */
}
else /* if autocal = 'y'(yes), 'q'(quiet), r(read), or 's'(semi) */
{
strcpy(codecseq,"Pdec_154p");
if(FIRST_FID) /* call Pbox */
{
ppm = getval("dfrq");
bw=20.0*ppm; ofs=154*ppm;
Pdec_154p = pbox_Dsh("Pdec_154p", "WURST2", bw, ofs, compC*pwC, pwClvl);
bw=30*ppm; ofs=0.0*ppm; nst = 1000; pws = 0.001;
me180 = pbox_makeA("me180", "sech", bw, pws, ofs, compC*pwC, pwClvl, nst);
}
copwr = Pdec_154p.pwr; pwco = 1.0/Pdec_154p.dmf;
cores = Pdec_154p.dres;
pwme180 = me180.pw; me180pwr= me180.pwr; me180pwrf = me180.pwrf;
}
/* CHECK VALIDITY OF PARAMETER RANGES */
if( gt1 > 0.5*del - 1.0e-4)
{
printf(" gt1 is too big. Make gt1 less than %f.\n", (0.5*del - 1.0e-4));
psg_abort(1);
}
if( dm[A] == 'y' )
{
printf("incorrect dec1 decoupler flag! Should be 'nny' or 'nnn' ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[C] == 'y'))
{
printf("incorrect dec2 decoupler flags! Should be 'nnn' ");
psg_abort(1);
}
if((dm3[A] == 'y' || dm3[C] == 'y'))
{
printf("incorrect dec3 decoupler flags! Should be 'nnn' or 'nyn' ");
psg_abort(1);
}
if( dpwr > 52 )
{
printf("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if( pw > 50.0e-6 )
{
printf("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwN > 100.0e-6 )
{
printf("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
/* PHASES AND INCREMENTED TIMES */
/* Phase incrementation for hypercomplex 2D data, States-Haberkorn element */
icosel1 = 1; icosel2 = 1;
if (phase1 == 2)
{ tsadd(t2,2,4); icosel1 = -1;}
if (phase2 == 2)
{ tsadd(t4,2,4); icosel2 = -1; tsadd(t2,2,4);}
/* Set up f1180 */
tau1 = d2;
if((f1180[A] == 'y') && (ni > 1.0))
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
tau1 = tau1/2.0;
/* Set up f2180 */
tau2 = d3;
if((f2180[A] == 'y') && (ni2 > 1.0))
{ tau2 += ( 1.0 / (2.0*sw2) ); if(tau2 < 0.2e-6) tau2 = 0.0; }
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{ tsadd(t1,2,4); tsadd(t11,2,4); }
if(ni > 1)
kappa = (double)(t1_counter*(del2)) / ( (double) (ni-1) );
else kappa = 0.0;
/* BEGIN PULSE SEQUENCE */
status(A);
decoffset(dof-140*dfrq);
obspower(tpwr);
decpower(pwClvl);
dec2power(pwNlvl);
decpwrf(rf0);
obsoffset(tof);
txphase(zero);
delay(1.0e-5);
if (satmode[A] == 'y')
{
obspower(satpwr);
txphase(zero);
rgpulse(d1,zero,20.0e-6,20.0e-6);
obspower(tpwr); /* Set power for hard pulses */
}
else
{
obspower(tpwr); /* Set power for hard pulses */
delay(d1);
}
decrgpulse(pwC, zero, 0.0, 0.0); /*destroy C13 magnetization*/
zgradpulse(gzlvl1, 0.5e-3);
delay(gstab);
decrgpulse(pwC, one, 0.0, 0.0);
zgradpulse(0.7*gzlvl1, 0.5e-3);
delay(1.1*gstab);
if(dm3[B] == 'y') /*optional 2H decoupling on */
{
dec3unblank();
dec3rgpulse(1/dmf3, one, 0.0, 0.0);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
}
rgpulse(pw, zero, 0.0, 0.0); /* 1H pulse excitation */
zgradpulse(gzlvl3, gt3);
decphase(zero);
delay(0.5*del - gt3);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl3, gt3);
txphase(one);
decphase(t1);
delay(0.5*del - gt3);
rgpulse(pw,one,0.0,0.0);
zgradpulse(1.8*gzlvl3, gt3);
txphase(zero);
delay(150e-6);
decrgpulse(pwC, t1, 0.0, 0.0);
/* decoupling on for carbonyl carbon */
decpwrf(4095.0);
decpower(copwr);
decprgon(codecseq,pwco,cores);
decon();
/* decoupling on for carbonyl carbon */
delay(tau1);
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
zgradpulse(icosel1*gzlvl4, gt1);
delay(0.5*del2 - 2.0*pwN - gt1 - 2.0*pw);
rgpulse(2.0*pw,zero,0.0,0.0);
delay(tau1 - (kappa*tau1));
/* co-decoupling off */
decoff();
decprgoff();
/* co-decoupling off */
decpower(pwClvl);
decrgpulse(2.0*pwC, zero, 0.0, 0.0);
/* decoupling on for carbonyl carbon */
decpwrf(4095.0);
decpower(copwr);
decprgon(codecseq,pwco,cores);
decon();
/* decoupling on for carbonyl carbon */
delay(0.5*del2 - kappa*tau1);
/* co-decoupling off */
decoff();
decprgoff();
/* co-decoupling off */
decpower(pwClvl);
decphase(t2);
decrgpulse(pwC, t2, 0.0, 0.0);
decpwrf(rfd);
delay(2.0e-6);
initval(ncyc, v2);
starthardloop(v2);
decrgpulse(4.9*p_d,one,0.0,0.0);
decrgpulse(7.9*p_d,three,0.0,0.0);
decrgpulse(5.0*p_d,one,0.0,0.0);
decrgpulse(5.5*p_d,three,0.0,0.0);
decrgpulse(0.6*p_d,one,0.0,0.0);
decrgpulse(4.6*p_d,three,0.0,0.0);
decrgpulse(7.2*p_d,one,0.0,0.0);
decrgpulse(4.9*p_d,three,0.0,0.0);
decrgpulse(7.4*p_d,one,0.0,0.0);
decrgpulse(6.8*p_d,three,0.0,0.0);
decrgpulse(7.0*p_d,one,0.0,0.0);
decrgpulse(5.2*p_d,three,0.0,0.0);
decrgpulse(5.4*p_d,one,0.0,0.0);
decrgpulse(0.6*p_d,three,0.0,0.0);
decrgpulse(4.5*p_d,one,0.0,0.0);
decrgpulse(7.3*p_d,three,0.0,0.0);
decrgpulse(5.1*p_d,one,0.0,0.0);
decrgpulse(7.9*p_d,three,0.0,0.0);
decrgpulse(4.9*p_d,three,0.0,0.0);
decrgpulse(7.9*p_d,one,0.0,0.0);
decrgpulse(5.0*p_d,three,0.0,0.0);
decrgpulse(5.5*p_d,one,0.0,0.0);
decrgpulse(0.6*p_d,three,0.0,0.0);
decrgpulse(4.6*p_d,one,0.0,0.0);
decrgpulse(7.2*p_d,three,0.0,0.0);
decrgpulse(4.9*p_d,one,0.0,0.0);
decrgpulse(7.4*p_d,three,0.0,0.0);
decrgpulse(6.8*p_d,one,0.0,0.0);
decrgpulse(7.0*p_d,three,0.0,0.0);
decrgpulse(5.2*p_d,one,0.0,0.0);
decrgpulse(5.4*p_d,three,0.0,0.0);
decrgpulse(0.6*p_d,one,0.0,0.0);
decrgpulse(4.5*p_d,three,0.0,0.0);
decrgpulse(7.3*p_d,one,0.0,0.0);
decrgpulse(5.1*p_d,three,0.0,0.0);
decrgpulse(7.9*p_d,one,0.0,0.0);
decrgpulse(4.9*p_d,three,0.0,0.0);
decrgpulse(7.9*p_d,one,0.0,0.0);
decrgpulse(5.0*p_d,three,0.0,0.0);
decrgpulse(5.5*p_d,one,0.0,0.0);
decrgpulse(0.6*p_d,three,0.0,0.0);
decrgpulse(4.6*p_d,one,0.0,0.0);
decrgpulse(7.2*p_d,three,0.0,0.0);
decrgpulse(4.9*p_d,one,0.0,0.0);
decrgpulse(7.4*p_d,three,0.0,0.0);
decrgpulse(6.8*p_d,one,0.0,0.0);
decrgpulse(7.0*p_d,three,0.0,0.0);
decrgpulse(5.2*p_d,one,0.0,0.0);
decrgpulse(5.4*p_d,three,0.0,0.0);
decrgpulse(0.6*p_d,one,0.0,0.0);
decrgpulse(4.5*p_d,three,0.0,0.0);
decrgpulse(7.3*p_d,one,0.0,0.0);
decrgpulse(5.1*p_d,three,0.0,0.0);
decrgpulse(7.9*p_d,one,0.0,0.0);
decrgpulse(4.9*p_d,one,0.0,0.0);
decrgpulse(7.9*p_d,three,0.0,0.0);
decrgpulse(5.0*p_d,one,0.0,0.0);
decrgpulse(5.5*p_d,three,0.0,0.0);
decrgpulse(0.6*p_d,one,0.0,0.0);
decrgpulse(4.6*p_d,three,0.0,0.0);
decrgpulse(7.2*p_d,one,0.0,0.0);
decrgpulse(4.9*p_d,three,0.0,0.0);
decrgpulse(7.4*p_d,one,0.0,0.0);
decrgpulse(6.8*p_d,three,0.0,0.0);
decrgpulse(7.0*p_d,one,0.0,0.0);
decrgpulse(5.2*p_d,three,0.0,0.0);
decrgpulse(5.4*p_d,one,0.0,0.0);
decrgpulse(0.6*p_d,three,0.0,0.0);
decrgpulse(4.5*p_d,one,0.0,0.0);
decrgpulse(7.3*p_d,three,0.0,0.0);
decrgpulse(5.1*p_d,one,0.0,0.0);
decrgpulse(7.9*p_d,three,0.0,0.0);
endhardloop();
txphase(one);
decpwrf(rf0);
decphase(t3);
obspower(tpwrd);
decrgpulse(pwC,t3,0.0,0.0);
decoffset(dof - 155*dfrq);
rgpulse(pwHd,one,0.0,2.0e-6);
txphase(zero);
obsunblank();
obsprgon("waltz16", pwHd, 90.0); /* PRG_START_DELAY */
xmtron();
delay(TC - OFFSET_DELAY - POWER_DELAY - PRG_START_DELAY - tau2);
decrgpulse(2.0*pwC, zero, 0.0, 0.0);
delay(TC + tau2 - POWER_DELAY - PRG_STOP_DELAY - 2*gt1 - gstab - 2.0*pw);
xmtroff();
obsprgoff();
obsblank();
rgpulse(pwHd,three,2.0e-6,0.0);
obspower(tpwr);
if (mag_flg[A] =='y')
magradpulse(gzcal*icosel2*gzlvl2, gt1);
else
zgradpulse(icosel2*gzlvl2, gt1);
delay(gstab/2.0);
rgpulse(2.0*pw,zero,0.0,0.0);
if (mag_flg[A] =='y')
magradpulse(gzcal*icosel2*gzlvl2, gt1);
else
zgradpulse(icosel2*gzlvl2, gt1);
delay(gstab/2.0);
decphase(zero);
simpulse(0.0,pwC, two, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
delay(0.5*del1 - gt5);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
txphase(one);
decphase(t4);
delay(0.5*del1 - gt5);
simpulse(pw, pwC, one, t4, 0.0, 0.0);
zgradpulse(gzlvl6, gt5);
txphase(zero);
decphase(zero);
delay(0.5*del4 - gt5);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl6, gt5);
delay(0.5*del4 - gt5);
simpulse(pw,pwC,zero,zero,0.0,0.0);
zgradpulse(2.3*gzlvl6, gt1);
if (autocal[A] == 'y')
{
decpower(me180pwr); decpwrf(me180pwrf);
delay(0.5*del3 - gt1 - 0.0005 -2.0*POWER_DELAY- WFG2_START_DELAY);
simshaped_pulse("","me180",2.0*pw,0.001, zero, zero, 0.0, 0.0);
decpwrf(rf0);
decphase(zero);
}
else
{
delay(0.5*del3 - 0.5*pwC - gt1);
simpulse(2.0*pw,2.0*pwC, zero, zero, 0.0, 0.0);
}
decpower(dpwr);
if (mag_flg[A] == 'y')
magradpulse(gzcal*((2.3*gzlvl6)+gzlvl1), gt1);
else
zgradpulse(((2.3*gzlvl6)+gzlvl1), gt1);
if (autocal[A] == 'y')
{
if(dm3[B] == 'y')
delay(0.5*del3 - 0.0005 -gt1 -1/dmf3 - 2.0*GRADIENT_DELAY - 2.0*POWER_DELAY);
else
delay(0.5*del3 - 0.0005 -gt1 - 2.0*GRADIENT_DELAY - 2.0*POWER_DELAY);
}
else
{
if(dm3[B] == 'y')
delay(0.5*del3 - gt1 -1/dmf3 - 2.0*GRADIENT_DELAY - POWER_DELAY);
else
delay(0.5*del3 - gt1 - 2.0*GRADIENT_DELAY - POWER_DELAY);
}
if(dm3[B] == 'y') /*optional 2H decoupling off */
{
dec3rgpulse(1/dmf3, three, 0.0, 0.0);
setstatus(DEC3ch, FALSE, 'w', FALSE, dmf3);
dec3blank();
}
if (dm3[B]=='y') lk_sample();
status(C);
setreceiver(t11);
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
double aXxyxy8 = getval("aXxyxy8");
double aYxyxy8 = getval("aYxyxy8");
double pwXxyxy8 = getval("pwXxyxy8");
double pwYxyxy8 = getval("pwYxyxy8");
double nXYxy8 = getval("nXYxy8");
int counter = nXYxy8;
initval(nXYxy8,v8);
double fXYxy8 = getval("fXYxy8");
double onYxyxy8 = getval("onYxyxy8");
double srate = getval("srate");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
DSEQ mix = getdseq("Hmix");
strncpy(mix.t.ch,"dec",3);
putCmd("chHmixtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHmixspinal='dec'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwX90") + nXYxy8*(pwXxyxy8 + pwYxyxy8);
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d.c3 = d.c3 + (!strcmp(mix.seq,"tppm"));
d.c3 = d.c3 + ((!strcmp(mix.seq,"tppm")) && (mix.t.a > 0.0));
d.t3 = nXYxy8*(1.0/srate - pwXxyxy8 - pwYxyxy8);
d.c4 = d.c4 + (!strcmp(mix.seq,"spinal"));
d.c4 = d.c4 + ((!strcmp(mix.seq,"spinal")) && (mix.s.a > 0.0));
d.t4 = nXYxy8*(1.0/srate - pwXxyxy8 - pwYxyxy8);
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phX90,4,table1);
settable(phXYxy8,8,table2);
settable(phXxyxy8,4,table3);
settable(phYxyxy8,4,table4);
settable(phRec,4,table5);
settable(ph1Rec,4,table6);
settable(ph2Rec,4,table7);
settable(ph3Rec,4,table8);
int tix = counter%8;
if ((tix == 1) || (tix == 7)) ttadd(phRec,ph1Rec,4);
if ((tix == 2) || (tix == 6)) ttadd(phRec,ph2Rec,4);
if ((tix == 3) || (tix == 5)) ttadd(phRec,ph3Rec,4);
setreceiver(phRec);
// Begin Sequence
txphase(phX90); decphase(zero);
obspwrf(getval("aX90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// X Direct Polarization
rgpulse(getval("pwX90"),phX90,0.0,0.0);
// xy8XY Period
if (counter > 0) {
_dseqon(mix);
delay(pwXxyxy8/2.0);
obspwrf(aXxyxy8); dec2pwrf(aYxyxy8);
sub(v1,v1,v1);
if (counter >= 1) {
loop(v8,v9);
getelem(phXYxy8,v1,v4);
incr(v1);
getelem(phXxyxy8,ct,v2);
getelem(phYxyxy8,ct,v3);
add(v4,v2,v2);
add(v4,v3,v3);
txphase(v2); dec2phase(v3);
delay((1.0 - fXYxy8)/srate - pwYxyxy8/2.0 - pwXxyxy8/2.0);
if (onYxyxy8 == 2)
dec2rgpulse(pwYxyxy8,v3,0.0,0.0);
else
delay(pwYxyxy8);
delay(fXYxy8/srate - pwYxyxy8/2.0 - pwXxyxy8/2.0);
rgpulse(pwXxyxy8,v2,0.0,0.0);
endloop(v9);
delay(1.0/srate - pwXxyxy8/2.0);
}
_dseqoff(mix);
}
// Begin Acquisition
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence()
{
double slpwrR = getval("slpwrR"),
slpwR = getval("slpwR"),
mixR = getval("mixR"),
gzlvlz = getval("gzlvlz"),
gtz = getval("gtz"),
zfphinc = getval("zfphinc");
char slpatR[MAXSTR];
int phase1 = (int)(getval("phase")+0.5);
/* LOAD AND INITIALIZE PARAMETERS */
getstr("slpatR",slpatR);
if (strcmp(slpatR,"cw") &&
strcmp(slpatR,"troesy") &&
strcmp(slpatR,"dante"))
abort_message("SpinLock pattern %s not supported!.\n", slpatR);
sub(ct,ssctr,v7);
settable(t1,4,ph1); getelem(t1,v7,v1);
settable(t2,8,ph2); getelem(t2,v7,v2);
settable(t3,8,ph3);
settable(t8,4,ph8); getelem(t8,v7,v8);
settable(t6,8,ph6); getelem(t6,v7,v6);
assign(v1,oph);
if (getflag("zfilt"))
getelem(t3,v7,oph);
add(v2,one,v3);
add(v2,two,v4);
add(v2,three,v5);
if (phase1 == 2)
{incr(v1); incr(v6);} /* hypercomplex method */
/*
mod2(id2,v13);
dbl(v13,v13);
*/
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v13);
add(v1,v13,v1);
add(v6,v13,v6);
add(oph,v13,oph);
/* The following is for flipback pulse */
zfphinc=zfphinc+180;
if (zfphinc < 0) zfphinc=zfphinc+360;
initval(zfphinc,v10);
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
if (satmode[0] == 'y')
{
if ((d1-satdly) > 0.02)
delay(d1-satdly);
else
delay(0.02);
satpulse(satdly,v6,rof1,rof1);
}
else
delay(d1);
if (getflag("wet"))
wet4(zero,one);
decpower(dpwr);
status(B);
rgpulse(pw, v1, rof1, rof1);
if (d2 > 0.0)
delay(d2 - POWER_DELAY - (2*pw/PI) - rof1);
else
delay(d2);
obspower(slpwrR);
if (mixR > 0.0)
{
if (dps_flag)
{
if (!strcmp(slpatR,"troesy"))
rgpulse(mixR,v2,0.0,0.0);
else
rgpulse(mixR,v3,0.0,0.0);
}
else
SpinLock(slpatR,mixR,slpwR,v2,v3,v4,v5, v9);
}
if ((getflag("zfilt")) && (getflag("PFGflg")))
{
obspower(tpwr);
rgpulse(pw,v2,1.0e-6,rof1);
zgradpulse(gzlvlz,gtz);
delay(gtz/3);
if (getflag("flipback"))
FBpulse(v8,v10);
rgpulse(pw,v8,rof1,rof2);
}
else
delay(rof2);
status(C);
}
pulsesequence()
{
double gzlvl1,qlvl,grise,gstab,gt1,ss,phase;
int icosel,iphase;
ss = getval("ss");
grise=getval("grise");
gstab=getval("gstab");
gt1=getval("gt1");
gzlvl1=getval("gzlvl1");
qlvl=getval("qlvl");
phase=getval("phase");
iphase = (int) (phase + 0.5);
/* DETERMINE STEADY-STATE MODE */
if (ss < 0) ss = (-1) * ss;
else
if ((ss > 0) && (ix == 1)) ss = ss;
else
ss = 0;
initval(ss, ssctr);
initval(ss, ssval);
assign(oph,v2);
assign(oph,v1);
if (iphase == 2)
incr(v1);
/* HYPERCOMPLEX MODE */
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v6);
if ((iphase==1) || (iphase==2))
{add(v1,v6,v1); add(oph,v6,oph);}
status(A);
rcvroff();
delay(d1);
if (getflag("wet")) wet4(zero,one);
rlpower(tpwr,TODEV);
rgpulse(pw,v1,rof1,rof2);
status(B);
if (d2 > rof1 + 4.0*pw/3.1416)
delay(d2 - rof1 - 4.0*pw/3.1416);
status(C);
rgpulse(pw,v2,rof1,rof2);
delay(gt1+2.0*grise+24.4e-6);
rgpulse(2.0*pw,v2,rof1,rof2);
icosel=-1;
rgradient('z',gzlvl1*(double)icosel);
delay(gt1+grise);
rgradient('z',0.0);
txphase(oph);
delay(grise);
rgpulse(pw,v2,rof1,rof2);
rgradient('z',gzlvl1*qlvl);
delay(gt1+grise);
rgradient('z',0.0);
delay(grise);
rcvron();
delay(gstab);
status(D);
}
pulsesequence()
{
char rcvrsflag[MAXSTR],
mrmode[MAXSTR];
double compH, Hdpwr, pw90,
pwx = getval("pwx"),
pwxlvl = getval("pwxlvl");
getstr("rcvrs",rcvrsflag);
getstr("mrmode",mrmode);
Hdpwr = 0.0; compH = 1.0; /* initialize the parameters */
if (strcmp(rcvrsflag,"yy"))
printf("rcvrs parameter should be set to 'yy'\n");
/* check decoupling modes */
if ((dm[A] == 'y') || (dm[B] == 'y') || (dm[C] == 'y') || (dm[D] == 'y') || (h**o[0] == 'y'))
{
printf("dm should be set to 'nnnn' and h**o should set to 'n'");
psg_abort(1);
}
if(mrmode[A] == 'd') /* make the decoupling waveform */
{
Hdpwr = getval("Hdpwr");
compH = getval("compH");
pw90 = getval("pw90");
if(FIRST_FID)
H1dec = pbox_mix("pansyW16", "WALTZ16", Hdpwr, pw90*compH, tpwr);
}
else if(mrmode[A] != 's')
mrmode[A] = 'p'; /* default is 'p' */
status(A);
delay(1.0e-4);
decpower(pwxlvl);
obspower(tpwr);
delay(d1);
status(B);
if(mrmode[A] != 'p') /* sequential acquisition */
{
rgpulse(pw, oph, rof1, rof2);
setactivercvrs("yn");
startacq(alfa);
acquire(np,1.0/sw);
endacq();
delay(1.0e-6);
if(mrmode[A] == 'd')
{
obsunblank();
pbox_xmtron(&H1dec);
}
delay(d2);
decrgpulse(pwx, oph, rof1, rof2);
status(C);
setactivercvrs("ny");
startacq(alfa);
acquire(np,1.0/sw);
endacq();
if(mrmode[A] == 'd')
{
pbox_xmtroff();
obsblank();
}
}
else
{
delay(d2);
simpulse(pw, pwx, oph, oph, rof1, rof2);
status(C);
}
}
pulsesequence()
{
char sspul[MAXSTR];
double pwClvl=getval("pwClvl");
/* LOAD VARIABLES AND CHECK CONDITIONS */
getstr("sspul", sspul);
/* STEADY-STATE PHASECYCLING */
/* This section determines if the phase calculations trigger off of (SS - SSCTR)
or off of CT */
ifzero(ssctr);
hlv(ct, v4);
mod4(ct, v3);
elsenz(ssctr);
sub(ssval, ssctr, v12); /* v12 = 0,...,ss-1 */
hlv(v12, v4);
mod4(v12, v3);
endif(ssctr);
/* CALCULATE PHASECYCLE */
/* The phasecycle first performs a 4-step cycle on the third pulse in order
to select for DQC. Second, the 2-step QIS cycle is added in. Third, a
2-step cycle for axial peak suppression is performed on the second pulse.
Fourth, a 2-step cycle for axial peak suppression is performed on the
first pulse. If P-type peaks only are being selected, the 2-step cycle
for P-type peak selection is performed on the first pulse immediately
after the 4-step cycle on the third pulse. */
hlv(v4, v4);
if (phase1 == 0)
{
assign(v4, v6);
hlv(v4, v4);
mod2(v6, v6); /* v6 = P-type peak selection in w1 */
}
hlv(v4, v2);
mod4(v4, v4); /* v4 = quadrature image suppression */
hlv(v2, v1);
mod2(v1, v1);
dbl(v1, v1);
mod2(v2, v2);
dbl(v2, v2);
dbl(v3, v5);
add(v3, v5, v5);
add(v1, v5, v5);
add(v2, v5, v5);
add(v4, v5, v5);
add(v4, v1, v1);
add(v4, v2, v2);
add(v4, v3, v3);
if (phase1 == 0)
{
add(v6, v1, v1);
add(v6, v5, v5);
}
if (phase1 == 2)
incr(v1);
if (phase1 == 3)
add(id2, v1, v1); /* adds TPPI increment to the phase of the
* first pulse */
assign(v5, oph);
/* FOR HYPERCOMPLEX, USE STATES-TPPI TO MOVE AXIALS TO EDGE */
if ((phase1==2)||(phase1==1))
{
initval(2.0*(double)(d2_index%2),v9); /* moves axials */
add(v1,v9,v1); add(oph,v9,oph);
}
/* BEGIN ACTUAL PULSE SEQUENCE CODE */
status(A);
if (sspul[0] == 'y')
{
obspower(pwClvl-12);
rgpulse(200*pw, one, 10.0e-6, 0.0e-6);
rgpulse(200*pw, zero, 0.0e-6, 1.0e-6);
obspower(pwClvl);
}
delay(d1);
status(B);
rgpulse(pw, v1, rof1, 1.0e-6);
if (d2>0.0)
delay(d2 - rof1 - 1.0e-6 -(4*pw)/3.1416);
rgpulse(pw, v2, rof1, 0.0);
rgpulse(pw, v3, 1.0e-6, rof2);
add(v3,one,v8);
delay(d3);
rgpulse(2.0*pw,v8,rof1,rof1);
delay(d3);
status(C);
}
pulsesequence()
{
/* DECLARE VARIABLES */
char autocal[MAXSTR], /* auto-calibration flag */
fsat[MAXSTR],
fscuba[MAXSTR],
f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
spco180a[MAXSTR],
spco90b[MAXSTR],
spco180b[MAXSTR],
cadecseq[MAXSTR];
int phase, phase2, ni, ni2, icosel, /* used to get n and p type */
t1_counter, /* used for states tppi in t1 */
t2_counter; /* used for states tppi in t2 */
double tau1, /* t1 delay */
tau2, /* t2 delay */
taua, /* ~ 1/4JCH = 1.7 ms */
tauc, /* ~ 1/4JCAC' = 3.6 ms */
taud, /* ~ 1/4JC'CA = 4.3, 4.4 ms */
taue, /* 1/4JC'N = 12.4 ms */
tauf, /* 1/4JNH = 2.25 ms */
BigTC, /* carbon constant time period */
BigTN, /* nitrogen constant time period */
pwn, /* PW90 for 15N pulse */
pwca90a, /* PW90 for 13C at dvhpwr */
pwca180a, /* PW180 for 13C at dvhpwra */
pwco180a,
pwca180b, /* PW180 for ca nucleus @ dvhpwrb */
pwco180b,
pwco90b, /* PW90 for co nucleus @ dhpwrb */
tsatpwr, /* low level 1H trans.power for presat */
tpwrml, /* power level for h decoupling */
pwmlev, /* h 90 pulse at tpwrml */
dhpwr, /* power level for 13C pulses on dec1
90 for part a of the sequence at 43 ppm */
dvhpwra, /* power level for 180 13C pulses at 43 ppm */
dhpwrb, /* power level for 13C pulses on dec1 - 54 ppm
90 for part b of the sequence*/
dvhpwrb, /* power level for 13C pulses on dec1 - 54 ppm
180 for part b of the sequence */
dhpwr2, /* high dec2 pwr for 15N hard pulses */
sw1, /* sweep width in f1 */
sw2, /* sweep width in f2 */
dofcacb, /* dof for dipsi part, 43 ppm */
pwcadec, /* seduce ca decoupling at dpwrsed */
dpwrsed, /* power level for seduce ca decoupling */
dressed, /* resoln for seduce decoupling = 2 */
dhpwrcoa, /* power level for pwco180a, 180 shaped C' */
sphase1, /* phase shift for off resonance C' 180 */
pwN, pwNlvl, /* N-15 RF calibration parameters */
pwC, compC, pwClvl, /* C-13 RF calibration parameters */
compH,waltzB1,
BigT1,
gt1,
gt2,
gt4,
gt5,
gt6,
gt7,
gt9,
gt10,
gstab,
gzlvl1,
gzlvl2,
gzlvl4,
gzlvl5,
gzlvl6,
gzlvl7,
gzlvl9,
gzlvl10;
/* variables commented out are already defined by the system */
/* LOAD VARIABLES */
getstr("autocal",autocal);
getstr("fsat",fsat);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("fscuba",fscuba);
taua = getval("taua");
tauc = getval("tauc");
taud = getval("taud");
taue = getval("taue");
tauf = getval("tauf");
BigTC = getval("BigTC");
BigTN = getval("BigTN");
pwN = getval("pwN");
pwNlvl = getval("pwNlvl");
pwn = getval("pwn");
tpwr = getval("tpwr");
tsatpwr = getval("tsatpwr");
tpwrml = getval("tpwrml");
dpwr = getval("dpwr");
dhpwr2 = getval("dhpwr2");
phase = (int) ( getval("phase") + 0.5);
phase2 = (int) ( getval("phase2") + 0.5);
sw1 = getval("sw1");
sw2 = getval("sw2");
dofcacb = getval("dofcacb");
ni = getval("ni");
ni2 = getval("ni2");
BigT1 = getval("BigT1");
sphase1 = getval("sphase1");
gt1 = getval("gt1");
gt2 = getval("gt2");
gt4 = getval("gt4");
gt5 = getval("gt5");
gt6 = getval("gt6");
gt7 = getval("gt7");
gt9 = getval("gt9");
gt10 = getval("gt10");
gstab = getval("gstab");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl4 = getval("gzlvl4");
gzlvl5 = getval("gzlvl5");
gzlvl6 = getval("gzlvl6");
gzlvl7 = getval("gzlvl7");
gzlvl9 = getval("gzlvl9");
gzlvl10 = getval("gzlvl10");
if(autocal[0]=='n')
{
getstr("spco180a",spco180a);
getstr("spco90b",spco90b);
getstr("spco180b",spco180b);
getstr("cadecseq",cadecseq);
pwca90a = getval("pwca90a");
pwca180a = getval("pwca180a");
pwco180a = getval("pwco180a");
pwca180b = getval("pwca180b");
pwco90b = getval("pwco90b");
pwco180b = getval("pwco180b");
dhpwr = getval("dhpwr");
dvhpwra = getval("dvhpwra");
dhpwrb = getval("dhpwrb");
dvhpwrb = getval("dvhpwrb");
dhpwrcoa = getval("dhpwrcoa");
dpwrsed = getval("dpwrsed");
dressed = getval("dressed");
pwcadec = getval("pwcadec");
pwmlev = getval("pwmlev");
}
else
{
waltzB1=getval("waltzB1");
pwmlev=1/(4.0*waltzB1);
compH = getval("compH");
tpwrml= tpwr - 20.0*log10(pwmlev/(compH*pw));
tpwrml= (int) (tpwrml + 0.5);
strcpy(spco180a,"Psed180_133p");
strcpy(spco90b,"Phard90co_118p");
strcpy(spco180b,"Phard180co_118p");
strcpy(cadecseq,"Pseduce1_lek");
if (FIRST_FID)
{
pwN = getval("pwN");
pwNlvl = getval("pwNlvl");
pwC = getval("pwC");
pwClvl = getval("pwClvl");
compC = getval("compC");
ca90 = pbox("cal", CA90, "", dfrq, compC*pwC, pwClvl);
ca180 = pbox("cal", CA180, "", dfrq, compC*pwC, pwClvl);
co180 = pbox(spco180a, CO180, CO180ps, dfrq, compC*pwC, pwClvl);
co90b = pbox(spco90b, CO90b, CA180ps, dfrq, compC*pwC, pwClvl);
co180b = pbox(spco180b, CO180b, CA180ps, dfrq, compC*pwC, pwClvl);
ca180b = pbox("cal", CA180b, "", dfrq, compC*pwC, pwClvl);
cadec = pbox(cadecseq, CADEC, CADECps, dfrq, compC*pwC, pwClvl);
w16 = pbox_dec("cal", "WALTZ16", tpwrml, sfrq, compH*pw, tpwr);
}
pwca90a = ca90.pw; dhpwr = ca90.pwr;
pwca180a = ca180.pw; dvhpwra = ca180.pwr;
pwco180a = co180.pw; dhpwrcoa = co180.pwr;
pwco90b = co90b.pw; dhpwrb = co90b.pwr;
pwco180b = co180b.pw;
pwca180b = ca180b.pw; dvhpwrb = ca180b.pwr;
pwcadec = 1.0/cadec.dmf; dpwrsed = cadec.pwr; dressed = cadec.dres;
pwmlev = 1.0/w16.dmf;
pwn=pwN; dhpwr2=pwNlvl;
}
/* LOAD PHASE TABLE */
settable(t1,1,phi1);
settable(t2,1,phi2);
settable(t3,4,phi3);
settable(t4,1,phi4);
settable(t5,2,phi5);
settable(t6,2,phi6);
settable(t7,1,phi7);
settable(t8,1,phi8);
settable(t9,8,phi9);
settable(t10,8,rec);
settable(t11,1,phi11);
settable(t12,1,phi12);
settable(t13,1,phi13);
/* CHECK VALIDITY OF PARAMETER RANGES */
if( 0.5*ni*1/(sw1) > BigTC - gt10 )
{
printf(" ni is too big\n");
psg_abort(1);
}
if( ni2*1/(sw2) > 2.0*BigTN )
{
printf(" ni2 is too big\n");
psg_abort(1);
}
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y'))
{
printf("incorrect dec1 decoupler flags! ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' ))
{
printf("incorrect dec2 decoupler flags! Should be 'nny' ");
psg_abort(1);
}
if( pwmlev < 30.0e-6 )
{
printf("too much power during proton mlev sequence\n");
psg_abort(1);
}
if( tpwrml > 53 )
{
printf("tpwrml is too high\n");
psg_abort(1);
}
if( tsatpwr > 6 )
{
printf("TSATPWR too large !!! ");
psg_abort(1);
}
if( dpwr > 50 )
{
printf("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if( dpwr2 > 50 )
{
printf("don't fry the probe, DPWR2 too large! ");
psg_abort(1);
}
if( dhpwr > 62 )
{
printf("don't fry the probe, DHPWR too large! ");
psg_abort(1);
}
if( dhpwrb > 62 )
{
printf("don't fry the probe, DHPWRB too large! ");
psg_abort(1);
}
if( dvhpwrb > 62 ) /* pwr level for dipsi */
{
printf("don't fry the probe, DVHPWRB too large! ");
psg_abort(1);
}
if( dhpwr2 > 62 )
{
printf("don't fry the probe, DHPWR2 too large! ");
psg_abort(1);
}
if( pw > 200.0e-6 )
{
printf("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwmlev > 200.0e-6 )
{
printf("dont fry the probe, pwmlev too high ! ");
psg_abort(1);
}
if( pwn > 200.0e-6 )
{
printf("dont fry the probe, pwn too high ! ");
psg_abort(1);
}
if( pwcadec > 500.0e-6 || pwcadec < 200.0e-6 )
{
printf("pwcadec outside reasonable limits: < 500e-6 > 200e-6 \n");
psg_abort(1);
}
if( dpwrsed > 45 )
{
printf("dpwrsed is too high\n");
psg_abort(1);
}
if( gt1 > 15e-3 || gt2 > 15e-3 || gt4 >=15e-3 || gt5 > 15e-3 || gt6 >= 15e-3 || gt7 >= 15e-3 || gt9 >= 15e-3 || gt10 >= 15e-3)
{
printf("all gti values must be < 15e-3\n");
psg_abort(1);
}
if(gt10 > 250.0e-6) {
printf("gt10 must be 250e-6\n");
psg_abort(1);
}
/* Phase incrementation for hypercomplex 2D data */
if (phase == 2)
tsadd(t3,1,4);
if (phase2 == 2) {
tsadd(t11,2,4);
icosel = 1;
}
else icosel = -1;
/* Set up f1180 tau1 = t1 */
tau1 = d2;
if(f1180[A] == 'y') {
tau1 += ( 1.0 / (2.0*sw1) );
if(tau1 < 0.2e-6) tau1 = 0.0;
}
tau1 = tau1/2.0;
/* Set up f2180 tau2 = t2 */
tau2 = d3;
if(f2180[A] == 'y') {
tau2 += ( 1.0 / (2.0*sw2) );
if(tau2 < 0.2e-6) tau2 = 0.0;
}
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2 ;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2) {
tsadd(t3,2,4);
tsadd(t10,2,4);
}
if( ix == 1) d3_init = d3 ;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2) {
tsadd(t8,2,4);
tsadd(t10,2,4);
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
decoffset(dofcacb); /* initially pulse at 43 ppm */
decpower(dhpwr); /* Set Dec1 power for hard 13C pulses */
dec2power(dhpwr2); /* Set Dec2 power for 15N hard pulses */
/* Presaturation Period */
if (fsat[A] == 'y')
{
obspower(tsatpwr); /* Set transmitter power for 1H presaturation */
delay(2.0e-5);
rgpulse(d1,zero,rof1,rof1);
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
delay(2.0e-5);
if(fscuba[A] == 'y')
{
delay(2.2e-2);
rgpulse(pw,zero,2.0e-6,0.0);
rgpulse(2*pw,one,2.0e-6,0.0);
rgpulse(pw,zero,2.0e-6,0.0);
delay(2.2e-2);
}
}
else
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
delay(d1);
txphase(zero);
delay(1.0e-5);
/* Begin Pulses */
status(B);
rcvroff();
delay(20.0e-6);
/* ensure that magnetization originates on H and not 13C */
decrgpulse(pwca90a,zero,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(gstab);
rgpulse(pw,zero,0.0,0.0); /* 90 deg 1H pulse */
decphase(t1);
delay(0.2e-6);
zgradpulse(gzlvl9,gt9);
delay(2.0e-6);
decpower(dvhpwra);
delay(taua - POWER_DELAY - gt2 - 2.2e-6); /* taua <= 1/4JCH */
simpulse(2*pw,pwca180a,zero,t1,0.0,0.0);
decpower(dhpwr);
delay(0.2e-6);
zgradpulse(gzlvl9,gt9);
delay(2.0e-6);
txphase(t2); decphase(t3);
delay(taua - POWER_DELAY - gt2 - 2.2e-6);
rgpulse(pw,t2,0.0,0.0);
decrgpulse(pwca90a,t3,2.0e-6,0.0);
delay(2.0e-6);
delay(tau1);
decpower(dhpwrcoa);
decshaped_pulse(spco180a,pwco180a,zero,4.0e-6,0.0);
dec2rgpulse(2*pwn,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl10,gt10);
delay(2.0e-6);
decpower(dvhpwra);
delay(0.80e-3 - gt10 - 4.0e-6 - 2*POWER_DELAY);
delay(0.2e-6);
rgpulse(2*pw,zero,0.0,0.0);
decphase(t4);
initval(1.0,v3);
decstepsize(0.0);
dcplrphase(v3);
delay(BigTC - 0.80e-3);
decrgpulse(pwca180a,t4,0.0,0.0);
dcplrphase(zero);
delay(2.0e-6);
zgradpulse(gzlvl10,gt10);
delay(2.0e-6);
delay(BigTC - tau1 + 2*pwn + 2*pw - 2*POWER_DELAY - gt10 - 4.0e-6);
delay(0.2e-6);
decpower(dhpwrcoa);
decshaped_pulse(spco180a,pwco180a,zero,4.0e-6,0.0); /* bloch seigert */
decpower(dhpwr);
decrgpulse(pwca90a,zero,2.0e-6,0.0);
txphase(one); delay(2.0e-6);
/* H decoupling on */
obspower(tpwrml);
obsprgon("waltz16",pwmlev,90.0);
xmtron(); /* TURN ME OFF DONT FORGET */
/* H decoupling on */
decpower(dhpwrcoa);
decshaped_pulse(spco180a,pwco180a,zero,4.0e-6,0.0); /* bloch seigert */
decphase(t5);
initval(1.0,v3);
decstepsize(0.0);
dcplrphase(v3);
delay(tauc - 3*POWER_DELAY - PRG_START_DELAY);
decpower(dvhpwra);
decrgpulse(pwca180a,t5,0.0,0.0);
dcplrphase(zero);
decpower(dhpwrcoa);
decshaped_pulse(spco180a,pwco180a,zero,4.0e-6,0.0);
decphase(zero);
decpower(dhpwr);
delay(tauc - 2*POWER_DELAY);
decrgpulse(pwca90a,zero,0.0,0.0);
/* H decoupling off */
xmtroff();
obsprgoff();
/* H decoupling off */
rgpulse(pwmlev,two,2.0e-6,0.0);
delay(0.2e-6);
decoffset(dof);
decpower(dhpwrb);
delay(0.2e-6);
zgradpulse(gzlvl4,gt4);
delay(gstab);
rgpulse(pwmlev,zero,2.0e-6,0.0);
txphase(one); delay(2.0e-6);
/* H decoupling on */
obspower(tpwrml);
obsprgon("waltz16",pwmlev,90.0);
xmtron();
/* H decoupling on */
decphase(t6);
delay(2.0e-6);
decshaped_pulse(spco90b,pwco90b,t6,0.0,0.0);
decphase(zero);
delay(taud - POWER_DELAY - 4.0e-6);
decpower(dvhpwrb);
decrgpulse(pwca180b,zero,4.0e-6,0.0);
decpower(dhpwrb);
delay(taue - taud - POWER_DELAY + 2*pwn);
/* adjust phase */
initval(1.0,v2);
decstepsize(sphase1);
dcplrphase(v2);
/* adjust phase */
decshaped_pulse(spco180b,pwco180b,zero,0.0,0.0);
dcplrphase(zero);
dec2rgpulse(2*pwn,zero,0.0,0.0);
delay(taue - 2*POWER_DELAY - 4.0e-6 - 4.0e-6);
decpower(dvhpwrb);
decrgpulse(pwca180b,zero,4.0e-6,0.0); /* bloch seigert */
decpower(dhpwrb);
decshaped_pulse(spco90b,pwco90b,t7,4.0e-6,0.0);
/* H decoupling off */
xmtroff();
obsprgoff();
/* H decoupling off */
rgpulse(pwmlev,two,2.0e-6,0.0);
delay(0.2e-6);
zgradpulse(gzlvl5,gt5);
delay(gstab);
rgpulse(pwmlev,zero,2.0e-6,0.0);
txphase(one); delay(2.0e-6);
/* H decoupling on */
obspower(tpwrml);
obsprgon("waltz16",pwmlev,90.0);
xmtron();
/* H decoupling on */
dec2rgpulse(pwn,t8,2.0e-6,0.0);
dec2phase(t9); decphase(zero);
/* seduce on */
decpower(dpwrsed);
decprgon(cadecseq,pwcadec,dressed);
decon();
/* seduce on */
delay(BigTN - tau2 + WFG_START_DELAY + WFG_STOP_DELAY + pwco180b);
/* seduce off */
decoff();
decprgoff();
decpower(dhpwrb);
/* seduce off */
dec2rgpulse(2*pwn,t9,0.0,0.0);
decshaped_pulse(spco180b,pwco180b,zero,0.0,0.0);
dec2phase(t11);
/* seduce on */
decpower(dpwrsed);
decprgon(cadecseq,pwcadec,dressed);
decon();
/* seduce on */
delay(BigTN + tau2 - 5.5e-3 - POWER_DELAY - PRG_STOP_DELAY - pwmlev - 2.0e-6);
/* H decoupling off */
xmtroff();
obsprgoff();
/* H decoupling off */
rgpulse(pwmlev,two,2.0e-6,0.0);
obspower(tpwr);
delay(2.5e-3);
/* seduce off */
decoff();
decprgoff();
decpower(dhpwrb);
/* seduce off */
delay(0.2e-6);
zgradpulse(gzlvl1,gt1);
delay(2.0e-6);
txphase(zero);
dec2phase(t11);
delay(3.0e-3 - gt1 - 2.2e-6 - 2.0*GRADIENT_DELAY);
sim3pulse(pw,0.0,pwn,zero,zero,t11,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
dec2phase(zero);
delay(tauf - gt7 - 2.2e-6);
sim3pulse(2*pw,0.0,2*pwn,zero,zero,zero,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
txphase(t12);
dec2phase(t13);
delay(tauf - gt7 - 2.2e-6);
sim3pulse(pw,0.0,pwn,t12,zero,t13,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
txphase(zero);
dec2phase(zero);
delay(tauf - gt7 - 2.2e-6);
sim3pulse(2*pw,0.0,2*pwn,zero,zero,zero,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
txphase(zero);
delay(tauf - gt7 - 2.2e-6);
rgpulse(pw,zero,0.0,0.0);
txphase(zero);
delay(BigT1);
rgpulse(2*pw,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(icosel*gzlvl2,gt1/10);
delay(2.0e-6);
delay(BigT1 - gt1/10 - POWER_DELAY - 4.0e-6 - 2*GRADIENT_DELAY);
dec2power(dpwr2); /* set power for 15N decoupling */
/* BEGIN ACQUISITION */
status(C);
setreceiver(t10);
}
pulsesequence()
{
/* DECLARE AND LOAD VARIABLES */
char f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
H2Opurge[MAXSTR], stCdec[MAXSTR], /* calls STUD+ waveforms from shapelib */
STUD[MAXSTR]; /* apply automatically calculated STUD decoupling */
int t1_counter, /* used for states tppi in t1 */
t2_counter; /* used for states tppi in t2 */
double tau1, /* t1 delay */
BPdpwrspinlock, /* user-defined upper limit for spinlock(Hz) */
BPpwrlimits, /* =0 for no limit, =1 for limit */
tau2, /* t2 delay */
ni = getval("ni"), ni2 = getval("ni2"),
stdmf = getval("dmf80"), /* dmf for 80 ppm of STUD decoupling */
rf80 = getval("rf80"), /* rf in Hz for 80ppm STUD+ */
taua = getval("taua"), /* time delays for CH coupling evolution */
taub = getval("taub"), tauc = getval("tauc"),
/* string parameter stCdec calls stud decoupling waveform from your shapelib. */
studlvl, /* coarse power for STUD+ decoupling */
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rf0, /* maximum fine power when using pwC pulses */
/* p_d is used to calculate the isotropic mixing on the Cab region */
p_d, /* 50 degree pulse for DIPSI-3 at rfd */
rfd, /* fine power for 9.0 kHz rf at 600MHz */
ncyc = getval("ncyc"), /* no. of cycles of DIPSI-3 */
spinlock = getval("spinlock"), /* DIPSI-3 Field Strength in Hz */
/* the following pulse length for the SLP pulse is automatically calculated */
/* by the macro "hcch_tocsy". The SLP pulse shape,"offC10" is called */
/* directly from your shapelib. */
pwC10 = getval("pwC10"), /* 180 degree selective sinc pulse on CO(174ppm) */
rf7, /* fine power for the pwC10 ("offC10") pulse */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
sw1 = getval("sw1"), sw2 = getval("sw2"),
gt0 = getval("gt0"), /* other gradients */
gt3 = getval("gt3"), gt4 = getval("gt4"), gt5 = getval("gt5"),
gt7 = getval("gt7"), gzlvl0 = getval("gzlvl0"), gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"), gzlvl5 = getval("gzlvl5"), gzlvl6 = getval("gzlvl6"),
gzlvl7 = getval("gzlvl7");
getstr("f1180", f1180);
getstr("f2180", f2180);
getstr("H2Opurge", H2Opurge);
getstr("STUD", STUD);
/* 80 ppm STUD+ decoupling */
strcpy(stCdec, "stCdec80");
studlvl = pwClvl + 20.0 * log10(compC * pwC * 4.0 * rf80);
studlvl = (int) (studlvl + 0.5);
P_getreal(GLOBAL,"BPpwrlimits",&BPpwrlimits,1);
P_getreal(GLOBAL,"BPdpwrspinlock",&BPdpwrspinlock,1);
/* LOAD PHASE TABLE */
settable(t3, 2, phi3);
settable(t5, 4, phi5);
settable(t9, 8, phi9);
settable(t11, 8, rec);
/* INITIALIZE VARIABLES */
if (BPpwrlimits > 0.5)
{
if (spinlock > BPdpwrspinlock)
{
spinlock = BPdpwrspinlock;
printf("spinlock too large, reset to user-defined limit (BPdpwrspinlock)");
psg_abort(1);
}
}
if (dpwrf < 4095)
{
printf("reset dpwrf=4095 and recalibrate C13 90 degree pulse");
psg_abort(1);
}
if ((pwC > (25.0e-6 * 600.0 / sfrq)) && (ncyc > 0.0))
{
printf("Increase pwClvl so that pwC < 25*600/sfrq");
psg_abort(1);
}
/* maximum fine power for pwC pulses */
rf0 = 4095.0;
/* 180 degree one-lobe sinc pulse on CO, null at Ca 139ppm away */
rf7 = (compC * 4095.0 * pwC * 2.0 * 1.65) / pwC10; /* needs 1.65 times more */
rf7 = (int) (rf7 + 0.5); /* power than a square pulse */
if (spinlock < 1000.0)
{
printf("Spinlock seems too low. Please check spinlock value ! ");
psg_abort(1);
}
/* dipsi-3 spinlock on CbCa */
p_d = (5.0) / (9.0 * 4.0 * spinlock); /* DIPSI-3 */
rfd = (compC * 4095.0 * pwC * 5.0) / (p_d * 9.0);
rfd = (int) (rfd + 0.5);
ncyc = (int) (ncyc + 0.5);
/*************************For Ultra-High Field Probes***************************/
if (sfrq>590.0)
{
if (ncyc>2)
{
if (pwC>15)
{
if (rfd > 2000)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>14)
{
if (rfd > 1800)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>13)
{
if (rfd > 1600)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>12)
{
if (rfd > 1400)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>11)
{
if (rfd > 1200)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (rfd > 1000)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
}
}
}
}
}
else
{
if (ncyc == 2)
{
if (pwC>15)
{
if (rfd > 2200)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>14)
{
if (rfd > 2000)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>13)
{
if (rfd > 1800)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>12)
{
if (rfd > 1600)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>11)
{
if (rfd > 1400)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (rfd > 1200)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
}
}
}
}
}
else
{
if (ncyc == 1)
{
if (pwC>15)
{
if (rfd > 2400)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>14)
{
if (rfd > 2200)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>13)
{
if (rfd > 2000)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>12)
{
if (rfd > 1800)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (pwC>11)
{
if (rfd > 1600)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
else
{
if (rfd > 1400)
{
printf("spinlock too large. Lower value for probe protection");
psg_abort(1);
}
}
}
}
}
}
}
}
}
}
/*********************End: For Ultra-High Field Probes***************************/
/* CHECK VALIDITY OF PARAMETER RANGES */
if ((dm[A] == 'y' || dm[B] == 'y'))
{
printf("incorrect dec1 decoupler flags! Should be 'nny' or 'nnn' ");
psg_abort(1);
}
if ((dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y'))
{
printf("incorrect dec2 decoupler flags! Should be 'nnn' ");
psg_abort(1);
}
if ((dm3[A] == 'y' || dm3[C] == 'y'))
{
printf("incorrect dec1 decoupler flags! Should be 'nyn' or 'nnn' ");
psg_abort(1);
}
if (dpwr > 52)
{
printf("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if (pw > 80.0e-6)
{
printf("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if (pwN > 100.0e-6)
{
printf("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
/* PHASES AND INCREMENTED TIMES */
/* Phase incrementation for hypercomplex 2D data, States-Haberkorn element */
if (phase1 == 2)
tsadd(t3, 1, 4);
if (phase2 == 2)
tsadd(t5, 1, 4);
/* C13 TIME INCREMENTATION and set up f1180 */
/* Set up f1180 */
tau1 = d2;
if (f1180[A] == 'y')
{
tau1 += (1.0 / (2.0 * sw1));
if (tau1 < 0.2e-6)
tau1 = 0.0;
}
tau1 = tau1 / 2.0;
/* Set up f2180 */
tau2 = d3;
if (f2180[A] == 'y')
{
tau2 += (1.0 / (2.0 * sw2));
if (tau2 < 0.2e-6)
tau2 = 0.0;
}
tau2 = tau2 / 2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if (ix == 1)
d2_init = d2;
t1_counter = (int) ((d2 - d2_init) * sw1 + 0.5);
if (t1_counter % 2)
{
tsadd(t3, 2, 4);
tsadd(t11, 2, 4);
}
if (ix == 1)
d3_init = d3;
t2_counter = (int) ((d3 - d3_init) * sw2 + 0.5);
if (t2_counter % 2)
{
tsadd(t5, 2, 4);
tsadd(t11, 2, 4);
}
/* BEGIN PULSE SEQUENCE */
status(A);
if (dm3[B] == 'y')
lk_sample();
if ((ni / sw1 - d2) > 0)
delay(ni / sw1 - d2); /*decreases as t1 increases for const.heating */
if ((ni2 / sw2 - d3) > 0)
delay(ni2 / sw2 - d3); /*decreases as t2 increases for const.heating */
delay(d1);
if (dm3[B] == 'y')
{
lk_hold();
lk_sampling_off();
} /*freezes z0 correction, stops lock pulsing */
rcvroff();
obspower(tpwr);
decpower(pwClvl);
dec2power(pwNlvl);
decpwrf(rf0);
obsoffset(tof);
txphase(t3);
delay(1.0e-5);
decrgpulse(pwC, zero, 0.0, 0.0); /*destroy C13 magnetization */
zgradpulse(gzlvl0, 0.5e-3);
delay(1.0e-4);
decrgpulse(pwC, one, 0.0, 0.0);
zgradpulse(0.7 * gzlvl0, 0.5e-3);
delay(5.0e-4);
if (dm3[B] == 'y') /* begins optional 2H decoupling */
{
dec3rgpulse(1 / dmf3, one, 10.0e-6, 2.0e-6);
dec3unblank();
dec3phase(zero);
delay(2.0e-6);
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
}
rgpulse(pw, t3, 0.0, 0.0); /* 1H pulse excitation */
zgradpulse(gzlvl0, gt0); /* 2.0*GRADIENT_DELAY */
decphase(zero);
delay(taua + tau1 - gt0 - 2.0 * GRADIENT_DELAY - 2.0 * pwC);
decrgpulse(2.0 * pwC, zero, 0.0, 0.0);
txphase(zero);
delay(tau1);
rgpulse(2.0 * pw, zero, 0.0, 0.0);
zgradpulse(gzlvl0, gt0);
txphase(one);
decphase(t5);
delay(taua - gt0);
rgpulse(pw, one, 0.0, 0.0);
zgradpulse(gzlvl3, gt3);
delay(2.0e-4);
decrgpulse(pwC, t5, 0.0, 0.0);
delay(tau2);
dec2rgpulse(2.0 * pwN, zero, 0.0, 0.0);
zgradpulse(gzlvl4, gt4); /* 2.0*GRADIENT_DELAY */
decphase(zero);
decpwrf(rf7);
delay(taub - 2.0 * pwN - gt4 - 2.0 * GRADIENT_DELAY);
decshaped_pulse("offC10", pwC10, zero, 0.0, 0.0);
txphase(zero);
decpwrf(rf0);
delay(taub - 2.0 * pw);
rgpulse(2.0 * pw, zero, 0.0, 0.0);
delay(tau2);
decrgpulse(2.0 * pwC, zero, 0.0, 0.0);
decpwrf(rf7);
delay(taub);
decshaped_pulse("offC10", pwC10, zero, 0.0, 0.0);
zgradpulse(gzlvl4, gt4); /* 2.0*GRADIENT_DELAY */
delay(taub - gt4 - 2.0 * GRADIENT_DELAY);
decpwrf(rfd);
decrgpulse(1.0e-3, zero, 0.0, 0.0);
if (ncyc>0)
{
initval(ncyc, v2);
starthardloop(v2);
decrgpulse(4.9 * p_d, one, 0.0, 0.0);
decrgpulse(7.9 * p_d, three, 0.0, 0.0);
decrgpulse(5.0 * p_d, one, 0.0, 0.0);
decrgpulse(5.5 * p_d, three, 0.0, 0.0);
decrgpulse(0.6 * p_d, one, 0.0, 0.0);
decrgpulse(4.6 * p_d, three, 0.0, 0.0);
decrgpulse(7.2 * p_d, one, 0.0, 0.0);
decrgpulse(4.9 * p_d, three, 0.0, 0.0);
decrgpulse(7.4 * p_d, one, 0.0, 0.0);
decrgpulse(6.8 * p_d, three, 0.0, 0.0);
decrgpulse(7.0 * p_d, one, 0.0, 0.0);
decrgpulse(5.2 * p_d, three, 0.0, 0.0);
decrgpulse(5.4 * p_d, one, 0.0, 0.0);
decrgpulse(0.6 * p_d, three, 0.0, 0.0);
decrgpulse(4.5 * p_d, one, 0.0, 0.0);
decrgpulse(7.3 * p_d, three, 0.0, 0.0);
decrgpulse(5.1 * p_d, one, 0.0, 0.0);
decrgpulse(7.9 * p_d, three, 0.0, 0.0);
decrgpulse(4.9 * p_d, three, 0.0, 0.0);
decrgpulse(7.9 * p_d, one, 0.0, 0.0);
decrgpulse(5.0 * p_d, three, 0.0, 0.0);
decrgpulse(5.5 * p_d, one, 0.0, 0.0);
decrgpulse(0.6 * p_d, three, 0.0, 0.0);
decrgpulse(4.6 * p_d, one, 0.0, 0.0);
decrgpulse(7.2 * p_d, three, 0.0, 0.0);
decrgpulse(4.9 * p_d, one, 0.0, 0.0);
decrgpulse(7.4 * p_d, three, 0.0, 0.0);
decrgpulse(6.8 * p_d, one, 0.0, 0.0);
decrgpulse(7.0 * p_d, three, 0.0, 0.0);
decrgpulse(5.2 * p_d, one, 0.0, 0.0);
decrgpulse(5.4 * p_d, three, 0.0, 0.0);
decrgpulse(0.6 * p_d, one, 0.0, 0.0);
decrgpulse(4.5 * p_d, three, 0.0, 0.0);
decrgpulse(7.3 * p_d, one, 0.0, 0.0);
decrgpulse(5.1 * p_d, three, 0.0, 0.0);
decrgpulse(7.9 * p_d, one, 0.0, 0.0);
decrgpulse(4.9 * p_d, three, 0.0, 0.0);
decrgpulse(7.9 * p_d, one, 0.0, 0.0);
decrgpulse(5.0 * p_d, three, 0.0, 0.0);
decrgpulse(5.5 * p_d, one, 0.0, 0.0);
decrgpulse(0.6 * p_d, three, 0.0, 0.0);
decrgpulse(4.6 * p_d, one, 0.0, 0.0);
decrgpulse(7.2 * p_d, three, 0.0, 0.0);
decrgpulse(4.9 * p_d, one, 0.0, 0.0);
decrgpulse(7.4 * p_d, three, 0.0, 0.0);
decrgpulse(6.8 * p_d, one, 0.0, 0.0);
decrgpulse(7.0 * p_d, three, 0.0, 0.0);
decrgpulse(5.2 * p_d, one, 0.0, 0.0);
decrgpulse(5.4 * p_d, three, 0.0, 0.0);
decrgpulse(0.6 * p_d, one, 0.0, 0.0);
decrgpulse(4.5 * p_d, three, 0.0, 0.0);
decrgpulse(7.3 * p_d, one, 0.0, 0.0);
decrgpulse(5.1 * p_d, three, 0.0, 0.0);
decrgpulse(7.9 * p_d, one, 0.0, 0.0);
decrgpulse(4.9 * p_d, one, 0.0, 0.0);
decrgpulse(7.9 * p_d, three, 0.0, 0.0);
decrgpulse(5.0 * p_d, one, 0.0, 0.0);
decrgpulse(5.5 * p_d, three, 0.0, 0.0);
decrgpulse(0.6 * p_d, one, 0.0, 0.0);
decrgpulse(4.6 * p_d, three, 0.0, 0.0);
decrgpulse(7.2 * p_d, one, 0.0, 0.0);
decrgpulse(4.9 * p_d, three, 0.0, 0.0);
decrgpulse(7.4 * p_d, one, 0.0, 0.0);
decrgpulse(6.8 * p_d, three, 0.0, 0.0);
decrgpulse(7.0 * p_d, one, 0.0, 0.0);
decrgpulse(5.2 * p_d, three, 0.0, 0.0);
decrgpulse(5.4 * p_d, one, 0.0, 0.0);
decrgpulse(0.6 * p_d, three, 0.0, 0.0);
decrgpulse(4.5 * p_d, one, 0.0, 0.0);
decrgpulse(7.3 * p_d, three, 0.0, 0.0);
decrgpulse(5.1 * p_d, one, 0.0, 0.0);
decrgpulse(7.9 * p_d, three, 0.0, 0.0);
endhardloop();
}
decrgpulse(9.0 * p_d / 5.0, t9, 2.0e-6, 0.0);
if (H2Opurge[A] == 'y')
{
obspwrf(1000);
rgpulse(900 * pw, zero, 0.0, 0.0);
rgpulse(500 * pw, one, 0.0, 0.0);
obspwrf(4095.0);
}
zgradpulse(gzlvl7, gt7);
decpwrf(rf0);
delay(50.0e-6);
rgpulse(pw, zero, 0.0, 0.0);
zgradpulse(gzlvl7, gt7 / 1.6);
decrgpulse(pwC, three, 100.0e-6, 0.0);
zgradpulse(gzlvl5, gt5);
decphase(zero);
delay(tauc - gt5);
simpulse(2.0 * pw, 2.0 * pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
delay(tauc - gt5);
decrgpulse(pwC, zero, 0.0, 0.0);
zgradpulse(gzlvl3, gt3);
if (dm3[B] == 'y') /* turns off 2H decoupling */
{
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3rgpulse(1 / dmf3, three, 2.0e-6, 2.0e-6);
dec3blank();
lk_autotrig(); /* resumes lock pulsing */
}
delay(2.0e-4);
rgpulse(pw, zero, 0.0, 0.0);
zgradpulse(gzlvl6, gt5);
delay(taua - gt5 + rof1);
simpulse(2.0 * pw, 2.0 * pwC, zero, zero, 0.0, rof1);
zgradpulse(gzlvl6, gt5);
if (STUD[A] == 'y')
decpower(studlvl);
else
decpower(dpwr);
dec2power(dpwr2);
delay(taua - gt5 - 2.0 * POWER_DELAY);
rgpulse(pw, zero, 0.0, rof2);
rcvron();
if (dm3[B] == 'y')
lk_sample();
setreceiver(t11);
if ((STUD[A] == 'y') && (dm[C] == 'y'))
{
decprgon(stCdec, 1.0 / stdmf, 1.0);
decon();
if (dm2[C] == 'y')
{
setstatus(DEC2ch, TRUE, dmm2[C], FALSE, dmf2);
}
}
else
status(C);
}
void pulsesequence()
{
/* DECLARE VARIABLES */
char autocal[MAXSTR], /* auto-calibration flag */
fsat[MAXSTR],
fscuba[MAXSTR],
f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
shib[MAXSTR], /* iburp for inversion during first inept */
Hshp[MAXSTR], /* proton inversion during chirp */
ddseq[MAXSTR],
shreb[MAXSTR], /* reburb hard during t2 */
co_shp[MAXSTR], /* shape of co 180 at 176 ppm */
CT_flg[MAXSTR],
codecseq[MAXSTR],
c180_flg[MAXSTR],
n_shift[MAXSTR],
shibca[MAXSTR],
shibcai[MAXSTR];
int phase, phase2, t2_counter, ni2, ni,
t1_counter; /* used for states tppi in t2,t1 */
double tau1, /* t1 delay */
tau2, /* t2 delay */
taua, /* ~ 1/4JCH = 1.7 ms; first inept */
mix, /* noesy mixing time */
TC, /* Variable CT period during t1 1/2JCC */
TC2, /* Variable CT period during t3 1/2JCC */
pwc, /* 90 c pulse at dhpwr */
tsatpwr, /* low level 1H trans.power for presat */
dhpwr, /* power level for high power 13C pulses on dec1 */
sw1, /* sweep width in f1 */
sw2, /* sweep width in f2 */
pwC,pwClvl,compC,pwN,pwNlvl,ppm,ofs,bw, /*used by Pbox */
d_ib,
pwib,
pwhshp,
pwd1, /* 2H flip back pulses */
d_reb,
pwreb,
ph_reb,
ph_reb1, /* only used if CT_flg=='y' and n_shift=='y' */
pwco180,
dhpwr2,
pwn,
d_co180,
pwcodec, /* carbon pw90 for seduce decoupling */
dpwrsed,
dressed,
d_ibca, /* power level for selective 13Ca pulse during CT-t2 */
pwibca, /* selective 13Ca pulse width */
gt1,
gt2,
gt3,
gt4,
gt5,
gt6,
gt7,
gt8,
gt9,
gt10,
gt11,
gt12,
gstab,
gzlvl1,
gzlvl2,
gzlvl3,
gzlvl4,
gzlvl5,
gzlvl6,
gzlvl7,
gzlvl8,
gzlvl9,
gzlvl10,
gzlvl11,
gzlvl12;
/* variables commented out are already defined by the system */
/* LOAD VARIABLES */
getstr("autocal",autocal);
getstr("fsat",fsat);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("fscuba",fscuba);
getstr("ddseq",ddseq);
getstr("n_shift",n_shift);
getstr("Hshp",Hshp);
getstr("CT_flg",CT_flg);
getstr("c180_flg",c180_flg);
compC = getval("compC"); pwN=getval("pwN"); pwNlvl=getval("pwNlvl");
pwC = getval("pwC"); pwClvl=getval("pwClvl");
pwhshp = getval("pwhshp");
taua = getval("taua");
mix = getval("mix");
TC = getval("TC");
pwc = getval("pwc");
tpwr = getval("tpwr");
tsatpwr = getval("tsatpwr");
dhpwr = getval("dhpwr");
dpwr = getval("dpwr");
phase = (int) ( getval("phase") + 0.5);
phase2 = (int) ( getval("phase2") + 0.5);
sw1 = getval("sw1");
sw2 = getval("sw2");
ni2 = getval("ni2");
ni = getval("ni");
pwd1 = getval("pwd1");
ph_reb = getval("ph_reb");
ph_reb1 = getval("ph_reb1");
TC2 = getval("TC2");
dhpwr2 = getval("dhpwr2");
pwn = getval("pwn");
setautocal();
if(autocal[0]=='n')
{
getstr("shreb",shreb);
getstr("shib",shib);
getstr("shibca",shibca);
getstr("shibcai",shibcai);
getstr("co_shp",co_shp);
getstr("codecseq",codecseq);
d_reb = getval("d_reb");
pwreb = getval("pwreb");
d_ib = getval("d_ib");
pwib = getval("pwib");
d_ibca = getval("d_ibca");
pwibca = getval("pwibca");
d_co180 = getval("d_co180");
pwco180 = getval("pwco180");
pwcodec = getval("pwcodec");
dpwrsed = getval("dpwrsed");
dressed = getval("dressed");
}
else
{
/*strcpy(Hshp,"hard"); former declarations using TNMR.h syntax
strcpy(shreb,"Preb_5p");
strcpy(shib,"Pib_1p5");
strcpy(shibca,"Pib_35p");
strcpy(shibcai,"Pib_35pi");
strcpy(co_shp,"Psed_156p");
strcpy(codecseq,"Pdec_156p");*/
strcpy(Hshp,"hard");
strcpy(shreb,"Preb_5p");
strcpy(shib,"Pib_1p5");
strcpy(shibca,"Pib_35p");
strcpy(shibcai,"Pib_35pi");
strcpy(co_shp,"Psed_156p");
strcpy(codecseq,"Pdec_156p");
if (FIRST_FID)
{
ppm = getval("dfrq");
/* These are former declarations (at top) using TNMR.h syntax */
/*REB180 "reburp 110p 5p"*/ /* RE-BURP 180 on Cab at 24.6 ppm, 5 ppm away */
/*IB180 "iburp2 24.4p 1.5p"*/ /* I-BURP 180 on Me at 21.1 ppm, 1.5 ppm away */
/*IBCA "iburp2 24.4p 35p"*/ /* I-BURP 180 on Cab at 54.6 ppm, 35 ppm away */
/*IBCAI "iburp2 24.4p 35p"*/ /* I-BURP 180 on Cab at 54.6 ppm, 35 ppm away */
/*CO180 "seduce 30p 156p"*/ /* SEDUCE 180 on C' at 175.6 ppm 156 ppm away */
/*CODEC "WURST2 20p/4m 156p"*/ /* WURST2 decoupling on C' at 175.6 ppm 156 ppm away */
/*REB180ps "-stepsize 0.5 -attn i"*/ /* seduce 180 shape parameters */
/*CODECps "-dres 1.0 -maxincr 20.0 -attn i"*/
/*co180 = pbox(co_shp, CO180, REB180ps, dfrq, compC*pwc, dhpwr);*/
/*ibcai = pbox(shibcai, IBCAI, REB180ps, dfrq, compC*pwc, dhpwr);*/
/*ibca = pbox(shibca, IBCA, REB180ps, dfrq, compC*pwc, dhpwr);*/
/*ib180 = pbox(shib, IB180, REB180ps, dfrq, compC*pwc, dhpwr);*/
/*reb = pbox(shreb, REB180, REB180ps, dfrq, compC*pwc, dhpwr);*/
/*COdec = pbox(codecseq, CODEC, CODECps, dfrq, compC*pwc, dhpwr);*/
bw = 110.0*ppm; ofs = 5.0*ppm;
Preb_5p = pbox_Rsh("Preb_5p", "reburp", bw , ofs, compC*pwC, pwClvl);
bw = 24.4*ppm; ofs = 1.5*ppm;
Pib_1p5 = pbox_Rsh("Pib_1p5", "iburp2", bw , ofs, compC*pwC, pwClvl);
bw = 24.4*ppm; ofs = 35*ppm;
Pib_35p = pbox_Rsh("Pib_35p", "iburp2", bw , ofs, compC*pwC, pwClvl);
bw = 24.4*ppm; ofs = 35*ppm;
Pib_35pi = pbox_Rsh("Pib_35pi", "iburp2", bw , ofs, compC*pwC, pwClvl);
bw = 30.0*ppm; ofs = 156*ppm;
Psed_156p = pbox_Rsh("Psed_156p", "seduce", bw , ofs, compC*pwC, pwClvl);
bw = 20.0*ppm; ofs = 156*ppm;
Pdec_156p = pbox_Dsh("Pdec_156p", "WURST2", bw , ofs, compC*pwC, pwClvl);
ofs_check(H1ofs, C13ofs, N15ofs, H2ofs);
}
d_reb = Preb_5p.pwr; pwreb = Preb_5p.pw;
d_ib = Pib_1p5.pwr; pwib = Pib_1p5.pw;
d_ibca = Pib_35p.pwr; pwibca = Pib_35p.pw;
d_co180 = Psed_156p.pwr; pwco180 = Psed_156p.pw;
dpwrsed = Pdec_156p.pwr; pwcodec = 1.0/Pdec_156p.dmf; dressed = Pdec_156p.dres;
pwc=pwC; dhpwr=pwClvl; pwn=pwN; dhpwr2=pwNlvl; pwhshp=2.0*pw;
pwd1=1/dmf3; pwhshp=2.0*pw;
}
gt1 = getval("gt1");
gt2 = getval("gt2");
gt3 = getval("gt3");
gt4 = getval("gt4");
gt5 = getval("gt5");
gt6 = getval("gt6");
gt7 = getval("gt7");
gt8 = getval("gt8");
gt9 = getval("gt9");
gt10 = getval("gt10");
gt11 = getval("gt11");
gt12 = getval("gt12");
gstab = getval("gstab");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl3 = getval("gzlvl3");
gzlvl4 = getval("gzlvl4");
gzlvl5 = getval("gzlvl5");
gzlvl6 = getval("gzlvl6");
gzlvl7 = getval("gzlvl7");
gzlvl8 = getval("gzlvl8");
gzlvl9 = getval("gzlvl9");
gzlvl10 = getval("gzlvl10");
gzlvl11 = getval("gzlvl11");
gzlvl12 = getval("gzlvl12");
/* LOAD PHASE TABLE */
settable(t1,4,phi1);
settable(t2,8,phi2);
settable(t7,2,phi7);
settable(t8,2,phi8);
settable(t9,8,rec);
/* CHECK VALIDITY OF PARAMETER RANGES */
if(TC/2.0 - 0.5*(ni-1)*1/(sw1) - POWER_DELAY - 4.0e-6
- WFG3_START_DELAY - pwco180
- WFG3_STOP_DELAY - pwd1
- gt4 - gstab -2.0e-6 - POWER_DELAY
- 4.0e-6 - WFG_START_DELAY < 0.2e-6)
{
printf(" ni is too big\n");
psg_abort(1);
}
if(CT_flg[A] == 'y' && n_shift[A] == 'n') {
if(TC2/2.0 - 0.5*(ni2-1)*1/(sw2) - POWER_DELAY - 4.0e-6
- WFG3_START_DELAY - pwco180
- WFG3_STOP_DELAY - pwd1
- gt10 - gstab -2.0e-6 - POWER_DELAY
- 4.0e-6 - WFG_START_DELAY < 0.2e-6)
{
printf(" ni2 is too big\n");
psg_abort(1);
}
}
if(CT_flg[A] == 'y' && n_shift[A] == 'y') {
if(TC2/2.0 - 0.5*(ni2-1)/sw2 - POWER_DELAY
- 4.0e-6 - WFG3_START_DELAY - pwco180
- WFG3_STOP_DELAY - 2.0e-6 - POWER_DELAY - WFG_START_DELAY
- 4.0e-6 - pwibca - WFG_STOP_DELAY - pwd1
- gt10 - gstab -2.0e-6 - POWER_DELAY
- 4.0e-6 - WFG3_START_DELAY < 0.2e-6)
{
printf(" ni2 is too big\n");
psg_abort(1);
}
}
if((dm[A] == 'y' || dm[B] == 'y' ))
{
printf("incorrect dec1 decoupler flags! ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y'))
{
printf("incorrect dec2 decoupler flags! ");
psg_abort(1);
}
if((dm3[A] == 'y' || dm3[B] == 'y' || dm3[C] == 'y'))
{
printf("incorrect dec3 decoupler flags! ");
psg_abort(1);
}
if( tsatpwr > 6 )
{
printf("TSATPWR too large !!! ");
psg_abort(1);
}
if( dpwr > 48 )
{
printf("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if( d_ib > 54 )
{
printf("don't fry the probe, d_ib too large! ");
psg_abort(1);
}
if( dpwr2 > 49 )
{
printf("don't fry the probe, DPWR2 too large! ");
psg_abort(1);
}
if( dpwr3 > 51 )
{
printf("don't fry the probe, DPWR3 too large! ");
psg_abort(1);
}
if( dhpwr > 63 )
{
printf("don't fry the probe, DHPWR too large! ");
psg_abort(1);
}
if( pw > 200.0e-6 )
{
printf("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwd1 < 100.0e-6 && pwd1 != 0.0)
{
printf("dont fry the probe, pwd1 too short and dpwr3 too high! ");
psg_abort(1);
}
if(d_co180 > 50)
{
printf("dont fry the probe, d_co180 is too high\n ");
psg_abort(1);
}
if(((pwco180 > 250e-6) || (pwco180 < 200e-6)) && (autocal[A] == 'n'))
{
printf("pwco180 is misset < 250 us > 200 us\n");
psg_abort(1);
}
if(dpwrsed > 45)
{
printf("dpwrsed is misset < 46\n");
psg_abort(1);
}
if(gt1 > 15e-3 || gt2 > 15e-3 || gt3 > 15e-3 ||
gt4 > 15e-3 || gt5 > 15e-3 || gt6 > 15e-3 ||
gt7 > 15e-3 || gt8 > 15e-3 || gt9 > 15e-3 ||
gt10 > 15e-3 || gt11 > 15e-3 || gt12 > 15e-3)
{
printf("gradients on for too long. Must be < 15e-3 \n");
psg_abort(1);
}
/* Phase incrementation for hypercomplex 2D data */
if (phase2 == 2) {
tsadd(t2,1,4);
}
if (phase == 2)
tsadd(t1,1,4);
/* Set up f2180 tau2 = t2 */
tau2 = d3;
if(CT_flg[A] == 'y') {
if(f2180[A] == 'y') {
tau2 += ( 1.0 / (2.0*sw2) );
}
}
if(CT_flg[A] == 'n' && n_shift[A] == 'n') {
if(f2180[A] == 'y') {
tau2 += ( 1.0 / (2.0*sw2) - 4.0/PI*pwc - POWER_DELAY
- PRG_START_DELAY - 4.0*pw - 4.0e-6 - 2.0*pwn
- PRG_STOP_DELAY - POWER_DELAY - 4.0e-6);
if(tau2 < 0.0 && ix == 1)
printf("tau2 start2 negative; decrease sw2\n");
}
if(f2180[A] == 'n') {
tau2 = ( tau2 - 4.0/PI*pwc - POWER_DELAY
- PRG_START_DELAY - 4.0*pw - 4.0e-6 - 2.0*pwn
- PRG_STOP_DELAY - POWER_DELAY - 4.0e-6);
}
}
if(CT_flg[A] == 'n' && n_shift[A] == 'y') {
if(f2180[A] == 'y') {
tau2 += ( 1.0 / (2.0*sw2) - 4.0/PI*pwn - POWER_DELAY
- PRG_START_DELAY - 4.0*pw - 4.0e-6
- PRG_STOP_DELAY - POWER_DELAY - 4.0e-6);
if(tau2 < 0.0 && ix == 1)
printf("tau2 start2 negative; decrease sw2\n");
}
if(f2180[A] == 'n') {
tau2 = ( tau2 - 4.0/PI*pwn - POWER_DELAY
- PRG_START_DELAY - 4.0*pw - 4.0e-6
- PRG_STOP_DELAY - POWER_DELAY - 4.0e-6);
}
}
if(tau2 < 0.4e-6) tau2 = 0.4e-6;
tau2 = tau2/2.0;
/* Set up f1180 tau1 = t1 */
tau1 = d2;
if(f1180[A] == 'y') {
tau1 += ( 1.0 / (2.0*sw1) );
}
if(tau1 < 0.4e-6) tau1 = 0.4e-6;
tau1 = tau1/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2 ;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2) {
tsadd(t1,2,4);
tsadd(t9,2,4);
}
if( ix == 1) d3_init = d3 ;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2) {
tsadd(t2,2,4);
tsadd(t9,2,4);
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tsatpwr); /* Set transmitter power for 1H presaturation */
decpower(dhpwr); /* Set Dec1 power for hard 13C pulses */
dec2power(dhpwr2); /* Set Dec2 power for hard 15N pulses */
dec3power(dpwr3); /* Set Dec3 power for 2H pulses */
/* Presaturation Period */
if (fsat[0] == 'y')
{
delay(2.0e-5);
rgpulse(d1,zero,2.0e-6,2.0e-6); /* presat */
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
delay(2.0e-5);
if(fscuba[0] == 'y')
{
delay(2.2e-2);
rgpulse(pw,zero,2.0e-6,0.0);
rgpulse(2*pw,one,2.0e-6,0.0);
rgpulse(pw,zero,2.0e-6,0.0);
delay(2.2e-2);
}
}
else
{
delay(d1);
}
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
txphase(zero);
dec2phase(zero);
decphase(zero);
delay(1.0e-5);
/* Begin Pulses */
status(B);
rcvroff();
lk_hold();
delay(20.0e-6);
/* first ensure that magnetization does infact start on H and not C */
decrgpulse(pwc,zero,2.0e-6,2.0e-6);
delay(2.0e-6);
zgradpulse(gzlvl1,gt1);
delay(gstab);
decpower(d_ib); /* set power for chirp during inept */
delay(4e-6);
/* this is the real start */
rgpulse(pw,zero,0.0,0.0); /* 90 deg 1H pulse */
delay(2.0e-6);
zgradpulse(gzlvl2,gt2);
delay(2.0e-6);
delay(taua - gt2 - 4.0e-6 - WFG2_START_DELAY); /* taua <= 1/4JCH */
simshaped_pulse(Hshp,shib,pwhshp,pwib,zero,zero,0.0,0.0);
decphase(zero);
txphase(one); decphase(t1);
decpower(dhpwr);
delay(2.0e-6);
zgradpulse(gzlvl2,gt2);
delay(2.0e-6);
delay(taua - gt2 - 4.0e-6 - WFG2_STOP_DELAY - POWER_DELAY);
rgpulse(pw,one,0.0,0.0);
txphase(zero);
delay(2.0e-6);
zgradpulse(gzlvl3,gt3);
delay(gstab);
decrgpulse(pwc,t1,0.0,0.0);
decphase(zero);
delay(tau1);
decpower(d_co180);
sim3shaped_pulse(Hshp,co_shp,"hard",pwhshp,pwco180,2.0*pwn,zero,zero,zero,4.0e-6,0.0);
delay(TC/2.0 - tau1 - POWER_DELAY - 4.0e-6 - WFG3_START_DELAY - pwco180
- WFG3_STOP_DELAY - pwd1
- gt4 - gstab -2.0e-6 - POWER_DELAY
- 4.0e-6 - WFG_START_DELAY);
dec3rgpulse(pwd1,zero,0.0,0.0);
delay(tau1);
delay(2.0e-6);
zgradpulse(gzlvl4,gt4);
delay(gstab);
initval(1.0,v3);
decstepsize(ph_reb);
dcplrphase(v3);
decpower(d_reb);
decshaped_pulse(shreb,pwreb,zero,4.0e-6,0.0);
dcplrphase(zero);
decphase(zero); decpower(dhpwr);
delay(2.0e-6);
zgradpulse(gzlvl4,gt4);
delay(gstab);
delay(TC/2.0 - tau1 - WFG_STOP_DELAY - POWER_DELAY
- gt4 - gstab -2.0e-6);
decrgpulse(pwc,zero,0.0,0.0);
dec3rgpulse(pwd1,two,4.0e-6,0.0);
delay(2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(gstab);
rgpulse(pw,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl6,gt6);
delay(gstab);
decpower(d_ib);
delay(taua - gt6 - 4.0e-6 - WFG2_START_DELAY - POWER_DELAY);
simshaped_pulse(Hshp,shib,pwhshp,pwib,zero,zero,0.0,0.0);
decphase(zero);
delay(2.0e-6);
zgradpulse(gzlvl6,gt6);
delay(gstab);
decpower(dhpwr);
txphase(one);
delay(taua - gt6 - gstab -2.0e-6 - POWER_DELAY - WFG2_STOP_DELAY);
rgpulse(pw,one,0.0,0.0);
txphase(zero);
delay(mix - gt7 - 352.0e-6);
decrgpulse(pwc,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl7,gt7);
delay(gstab);
decpower(d_ib); /* set power level for iburp */
rgpulse(pw,zero,0.0,0.0); /* 90 deg 1H pulse */
delay(2.0e-6);
zgradpulse(gzlvl8,gt8);
delay(2.0e-6);
if(n_shift[A] == 'n') {
delay(taua - gt8 - 4.0e-6 - WFG2_START_DELAY); /* taua <= 1/4JCH */
simshaped_pulse(Hshp,shib,pwhshp,pwib,zero,zero,0.0,0.0);
decphase(zero);
}
else {
delay(taua - gt8 - 4.0e-6 - WFG3_START_DELAY);
sim3shaped_pulse(Hshp,shib,"hard",pwhshp,pwib,2.0*pwn,zero,zero,zero,0.0,0.0);
}
txphase(one); decphase(t2);
decpower(dhpwr);
delay(2.0e-6);
zgradpulse(gzlvl8,gt8);
delay(2.0e-6);
if(n_shift[A] == 'n')
delay(taua - gt8 - 4.0e-6 - WFG2_STOP_DELAY - POWER_DELAY);
else
delay(taua - gt8 - 4.0e-6 - WFG3_STOP_DELAY - POWER_DELAY);
rgpulse(pw,one,0.0,0.0);
txphase(zero);
delay(2.0e-6);
zgradpulse(gzlvl9,gt9);
delay(gstab);
if(CT_flg[A] == 'y' && n_shift[A] == 'n') {
decrgpulse(pwc,t2,0.0,0.0);
decphase(zero);
delay(tau2);
decpower(d_co180);
sim3shaped_pulse(Hshp,co_shp,"hard",pwhshp,pwco180,2.0*pwn,zero,zero,zero,4.0e-6,0.0);
delay(TC2/2.0 - tau2 - POWER_DELAY - 4.0e-6 - WFG3_START_DELAY - pwco180
- WFG3_STOP_DELAY - pwd1
- gt10 - gstab -2.0e-6 - POWER_DELAY
- 4.0e-6 - WFG_START_DELAY);
dec3rgpulse(pwd1,zero,0.0,0.0);
delay(tau2);
delay(2.0e-6);
zgradpulse(gzlvl10,gt10);
delay(gstab);
initval(1.0,v4);
decstepsize(ph_reb);
dcplrphase(v4);
decpower(d_reb);
decshaped_pulse(shreb,pwreb,zero,4.0e-6,0.0);
dcplrphase(zero);
decphase(zero); decpower(dhpwr);
delay(2.0e-6);
zgradpulse(gzlvl10,gt10);
delay(gstab);
delay(TC2/2.0 - tau2 - WFG_STOP_DELAY - POWER_DELAY
- gt10 - gstab -2.0e-6);
decrgpulse(pwc,zero,0.0,0.0);
dec3rgpulse(pwd1,two,4.0e-6,0.0);
}
if(CT_flg[A] == 'y' && n_shift[A] == 'y') {
dec2phase(t2); delay(2.0e-6);
dec2rgpulse((pwn-pwc)/2.0,t2,0.0,0.0);
sim3pulse(0.0e-6,pwc,pwc,zero,t2,t2,0.0,0.0);
dec2rgpulse((pwn-pwc)/2.0,t2,0.0,0.0);
decphase(zero);
delay(tau2);
decphase(zero);
decpower(d_co180);
sim3shaped_pulse(Hshp,co_shp,"hard",pwhshp,pwco180,0.0e-6,zero,zero,zero,4.0e-6,2.0e-6);
decpower(d_ibca);
decshaped_pulse(shibca,pwibca,zero,4.0e-6,0.0);
decphase(zero);
delay(TC2/2.0 - tau2 - POWER_DELAY - 4.0e-6 - WFG3_START_DELAY - pwco180
- WFG3_STOP_DELAY - 2.0e-6 - POWER_DELAY - WFG_START_DELAY
- 4.0e-6 - pwibca - WFG_STOP_DELAY - pwd1
- gt10 - gstab -2.0e-6 - POWER_DELAY
- 4.0e-6 - WFG3_START_DELAY);
dec3rgpulse(pwd1,zero,0.0,0.0);
delay(tau2);
delay(2.0e-6);
zgradpulse(gzlvl10,gt10);
delay(gstab);
initval(1.0,v4);
decstepsize(ph_reb1);
dcplrphase(v4);
decpower(d_reb);
sim3shaped_pulse("hard",shreb,"hard",0.0e-6,pwreb,2.0*pwn,zero,zero,zero,4.0e-6,0.0);
dcplrphase(zero);
decphase(t7); decpower(d_ibca);
decshaped_pulse(shibcai,pwibca,t7,4.0e-6,0.0);
decpower(dhpwr); decphase(zero);
delay(2.0e-6);
zgradpulse(gzlvl10,gt10);
delay(gstab);
delay(TC2/2.0 - tau2 - WFG3_STOP_DELAY - POWER_DELAY
- 4.0e-6 - WFG_START_DELAY - pwibca - WFG_STOP_DELAY
- POWER_DELAY
- gt10 - gstab -2.0e-6);
dec2rgpulse((pwn-pwc)/2.0,zero,0.0,0.0);
sim3pulse(0.0e-6,pwc,pwc,zero,zero,zero,0.0,0.0);
dec2rgpulse((pwn-pwc)/2.0,zero,0.0,0.0);
dec3rgpulse(pwd1,two,4.0e-6,0.0);
}
if(CT_flg[A] == 'n' && n_shift[A] == 'n') {
txphase(one);
decrgpulse(pwc,t2,0.0,0.0);
if(c180_flg[A] == 'n')
{
decphase(zero);
/* seduce on */
decpower(dpwrsed);
decprgon(codecseq,pwcodec,dressed);
decon();
/* seduce on */
delay(tau2);
rgpulse(pw,one,0.0,0.0);
rgpulse(2.0*pw,zero,2.0e-6,0.0);
rgpulse(pw,one,2.0e-6,0.0);
dec2rgpulse(2.0*pwn,zero,0.0,0.0);
delay(tau2);
/* seduce off */
decoff();
decprgoff();
decpower(dhpwr);
/* seduce off */
}
else
decrgpulse(2.0*pwc,zero,4.0e-6,0.0);
decrgpulse(pwc,zero,4.0e-6,0.0);
}
if(CT_flg[A] == 'n' && n_shift[A] == 'y') {
txphase(one); dec2phase(t2);
dec2rgpulse((PI-2.0)/PI*(pwn-pwc),t2,2.0e-6,0.0);
sim3pulse(0.0e-6,pwc,pwc,zero,t2,t2,0.0,0.0);
dec2rgpulse((2.0/PI)*(pwn-pwc),t2,0.0,0.0);
if(c180_flg[A] == 'n')
{
decphase(zero);
/* seduce on */
decpower(dpwrsed);
decprgon(codecseq,pwcodec,dressed);
decon();
/* seduce on */
delay(tau2);
rgpulse(pw,one,0.0,0.0);
rgpulse(2.0*pw,zero,2.0e-6,0.0);
rgpulse(pw,one,2.0e-6,0.0);
delay(tau2);
/* seduce off */
decoff();
decprgoff(); /* note that ca-n evolves ; keep t2,max <= 9.5ms */
decpower(dhpwr);
/* seduce off */
}
else
sim3pulse(0.0,2.0*pwc,2.0*pwn,zero,zero,zero,4.0e-6,0.0);
dec2rgpulse((2.0/PI)*(pwn-pwc),zero,4.0e-6,0.0);
sim3pulse(0.0e-6,pwc,pwc,zero,zero,zero,0.0,0.0);
dec2rgpulse((PI-2.0)/PI*(pwn-pwc),zero,0.0,0.0);
}
delay(2.0e-6);
zgradpulse(gzlvl11,gt11);
delay(gstab);
lk_sample();
rgpulse(pw,t8,4.0e-6,0.0); /* 90 deg 1H pulse */
delay(2.0e-6);
zgradpulse(gzlvl12,gt12);
delay(2.0e-6);
decpower(d_ib);
if(n_shift[A] == 'n') {
delay(taua - gt12 - 4.0e-6 - WFG2_START_DELAY - POWER_DELAY);
simshaped_pulse(Hshp,shib,pwhshp,pwib,t8,zero,0.0,0.0);
decphase(zero);
}
else {
delay(taua - gt12 - 4.0e-6 - WFG3_START_DELAY - POWER_DELAY);
sim3shaped_pulse(Hshp,shib,"hard",pwhshp,pwib,2.0*pwn,t8,zero,zero,0.0,0.0);
}
delay(2.0e-6);
zgradpulse(gzlvl12,gt12);
delay(2.0e-6);
if(n_shift[A] == 'n')
delay(taua - gt12 - 4.0e-6 - WFG2_STOP_DELAY - 2.0*POWER_DELAY);
else
delay(taua - gt12 - 4.0e-6 - WFG3_STOP_DELAY - 2.0*POWER_DELAY);
decpower(dpwr); /* Set power for decoupling */
dec2power(dpwr2); /* Set power for decoupling */
rgpulse(pw,t8,0.0,rof2);
/* BEGIN ACQUISITION */
status(C);
setreceiver(t9);
}
pulsesequence()
{
/* DECLARE VARIABLES */
char
SE[MAXSTR], /* coherence gradients & sensitivity enhance */
CT[MAXSTR], /* constant time in t1 */
CCdseq[MAXSTR],
CChomodec[MAXSTR], /* Setup for C-imino - C-H6 */
C13refoc[MAXSTR], /* C13 pulse in middle of t1*/
f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR]; /* Flag to start t1 @ halfdwell */
int icosel,
ni2 = getval("ni2"),
t1_counter,
t2_counter;
double tau1, /* t1 delay */
tau2, /* t2 delay */
lambda = 0.94/(4.0*getval("JCH")), /* 1/4J C-H INEPT delay */
CTdelay = getval("CTdelay"), /* total constant time evolution */
CCdpwr = getval("CCdpwr"), /* power level for CC decoupling */
CCdres = getval("CCdres"), /* dres for CC decoupling */
CCdmf = getval("CCdmf"), /* dmf for CC decoupling */
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
pwZa, /* the largest of 2.0*pw and 2.0
*pwN */
tpwr = getval("tpwr"), /* power for H1 pulses */
pw = getval("pw"), /* H1 90 degree pulse length at tpwr */
compH = getval("compH"), /* adjustment for H1 amplifier compression */
ncyc_cc = getval("ncyc_cc"), /* number of DIPSI3 cycles for CC spinlock */
tof_75, /* tof shifted to 7.5 ppm for H4-N4 transfer */
tof_12, /* tof shifted to 12 ppm for H3-N3 transfer */
dof_80, /* dof shifted to 169 ppm for N3-C4 transfer */
dof_92p5, /* dof shifted to 92.5ppm */
/* p_d is used to calculate the isotropic mixing */
p_d, /* 50 degree pulse for DIPSI-3 at rfdC */
p_d2, /* 50 degree pulse for DIPSI-3 at rfd */
rfd, /* fine C13 power for 10 kHz rf for 500MHz magnet */
sw1 = getval("sw1"),
sw2 = getval("sw2"),
gstab = getval("gstab"),
pwHs = getval("pwHs"), /* H1 90 degree pulse length at tpwrs */
tpwrs, /* power for the pwHs ("rna_H2Osinc") pulse */
pwHs2 = getval("pwHs2"), /* H1 90 degree pulse length at tpwrs2 */
tpwrs2, /* power for the pwHs2 square pulse */
gt1 = getval("gt1"), /* coherence pathway gradients */
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gzlvl0 = getval("gzlvl0"),
gzlvl3 = getval("gzlvl3"),
gt3 = getval("gt3"),
gzlvl4 = getval("gzlvl4"),
gt4 = getval("gt4"),
gzlvl5 = getval("gzlvl5"),
gzlvl6 = getval("gzlvl6"),
gt5 = getval("gt5"),
gzlvlr = getval("gzlvlr");
getstr("SE",SE);
getstr("CT",CT);
getstr("CChomodec",CChomodec);
getstr("CCdseq",CCdseq);
getstr("C13refoc",C13refoc);
getstr("f1180",f1180);
getstr("f2180",f2180);
/* LOAD PHASE TABLE */
settable(t1,2,phi1);
settable(t3,8,phi3);
settable(t4,16,phi4);
settable(t9,1,phi9);
settable(t10,1,phi10);
settable(t5,4,phi5);
settable(t11,8,rec1);
/* INITIALIZE VARIABLES */
/* different offset values tof=H2O, dof=110ppm, dof2=200ppm */
tof_75 = tof + 2.5*sfrq; /* tof shifted to nH2 */
tof_12 = tof + 8.0*sfrq; /* tof shifted to nH */
dof_92p5 = dof - 17.5*dfrq; /* dof shifted to C1' */
dof_80 = dof - 30*dfrq; /* dof shifted to C6 */
/* 1.9 kHz DIPSI-3 at 500MHz scaled to this sfrq*/
p_d = (5.0)/(9.0*4.0*1900.0*(sfrq/500.0));
/* 7 kHz DIPSI-3 at 500MHz scaled to this sfrq*/
p_d2 = (5.0)/(9.0*4.0*7000.0*(sfrq/500.0));
ncyc_cc = (int) (ncyc_cc + 0.5);
if (ncyc_cc > 0 )
{
printf("CC-mixing time is %f ms.\n",(ncyc_cc*51.8*4*p_d2));
}
if( ncyc_cc > 12 )
{
text_error("check C->C dipsi-3 time !! ");
psg_abort(1);
}
initval(ncyc_cc,v2);
/* fine C13 power for dipsi-3 isotropic mixing */
rfd = (compC*4095.0*pwC*5.0)/(p_d2*9.0);
rfd = (int) (rfd + 0.5);
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /* needs 1.69 times more */
tpwrs = (int) (tpwrs); /* power than a square pulse */
/* selective H20 square pulse */
tpwrs2 = tpwr - 20.0*log10(pwHs2/(compH*pw));
tpwrs2 = (int) (tpwrs2);
if (2.0*pw > 2.0*pwN) pwZa = 2.0*pw;
else pwZa = 2.0*pwN;
if ((CT[A]=='y') && (ni2/(4.0*sw2) > CTdelay))
{ text_error( " ni2 is too big. Make ni2 equal to %d or less.\n",
((int)(CTdelay*sw2*4.0)) ); psg_abort(1); }
/* PHASES AND INCREMENTED TIMES */
/* Phase incrementation for hypercomplex 2D data, States-Haberkorn element */
if (phase1 == 2)
tsadd(t5,1,4);
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{ tsadd(t5,2,4); tsadd(t11,2,4); }
/* Set up f1180 */
tau1 = d2;
if((f1180[A] == 'y') && (ni > 1.0))
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
tau1 = tau1/2.0;
icosel=1;
if (SE[A]=='y')
{
if (phase2 == 2)
{
tsadd(t10,2,4);
icosel = -1;
}
}
/* Set up f2180 */
tau2 = d3;
if((f2180[A] == 'y') && (ni2 > 1.0))
{
tau2 += ( 1.0 / (2.0*sw2) );
if(tau2 < 0.2e-6) tau2 = 0.0;
}
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d3_init = d3;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2)
{
tsadd(t3,2,4);
tsadd(t11,2,4);
}
/* CHECK VALIDITY OF PARAMETER RANGE */
if( sfrq > 610 )
{ printf("Power Levels at 750/800 MHz may be too high for probe");
psg_abort(1); }
if( dpwrf < 4095 )
{ printf("reset dpwrf=4095 and recalibrate C13 90 degree pulse");
psg_abort(1); }
if( dpwrf2 < 4095 )
{ printf("reset dpwrf2=4095 and recalibrate N15 90 degree pulse");
psg_abort(1); }
if((dm[A] == 'y' || dm[B] == 'y'))
{
printf("incorrect dec1 decoupler flag! Should be 'nny' or 'nnn' ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y'))
{
printf("incorrect dec2 decoupler flags! Should be 'nnn' or 'nny' ");
psg_abort(1);
}
if( ((dm[C] == 'y') && (dm2[C] == 'y') && (at > 0.18)) )
{
text_error("check at time! Don't fry probe !! ");
psg_abort(1);
}
if( dpwr > 50 )
{
printf("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if( dpwr2 > 50 )
{
printf("don't fry the probe, DPWR2 too large! ");
psg_abort(1);
}
if( pw > 20.0e-6 )
{
printf("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwC > 40.0e-6 )
{
printf("dont fry the probe, pwC too high ! ");
psg_abort(1);
}
if( pwN > 100.0e-6 )
{
printf("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
if (gzlvlr > 500 || gzlvlr < -500)
{
text_error(" RDt1-gzlvlr must be -500 to 500 (0.5G/cm) \n");
psg_abort(1);
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
obsstepsize(0.5);
decpower(pwClvl);
decstepsize(0.5);
obsoffset(tof);
dec2power(pwNlvl);
dec2stepsize(0.5);
decoffset(dof_80); /* Preset the carbon frequency for the C1' carbons */
txphase(zero);
decphase(zero);
dec2phase(zero);
delay(d1);
rcvroff();
dec2rgpulse(pwN, zero, 0.0, 0.0); /*destroy N15 and C13 magnetization*/
decrgpulse(pwC, zero, 0.0, 0.0);
zgradpulse(gzlvl0, 0.5e-3);
delay(1.0e-4);
dec2rgpulse(pwN, one, 0.0, 0.0);
decrgpulse(pwC, one, 0.0, 0.0);
zgradpulse(0.7*gzlvl0, 0.5e-3);
delay(5.0e-4);
delay(lambda);
simpulse(2*pw, 2*pwC, zero, zero, 0.0, 0.0);
dec2phase(t5);
delay(lambda - SAPS_DELAY);
simpulse(pw, pwC, zero, t5, 0.0, 0.0); /* 2x, -2x*/
dec2phase(zero);
txphase(one);
zgradpulse(gzlvl5,gt5);
delay(lambda - SAPS_DELAY - gt5);
simpulse(2*pw, 2*pwC, one, zero, 0.0, 0.0);
zgradpulse(gzlvl5,gt5);
delay(lambda - 2*SAPS_DELAY - gt5 - 2*POWER_DELAY);
decpwrf(4095.0);
txphase(zero);
decphase(zero);
if (C13refoc[A]=='y')
{
if (tau1 > (2.0*GRADIENT_DELAY + pwN + 0.64*pw + 5.0*SAPS_DELAY))
{
zgradpulse(gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw) - SAPS_DELAY);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(-1.0*gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
}
else if (tau1 > (0.64*pw + 0.5*SAPS_DELAY))
delay(2.0*tau1 - 2.0*0.64*pw - SAPS_DELAY );
}
else
{
if (tau1 > (2.0*GRADIENT_DELAY + pwN + 0.64*pw + 5.0*SAPS_DELAY))
{
zgradpulse(gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw) - SAPS_DELAY);
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
zgradpulse(-1.0*gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
}
else if (tau1 > (0.64*pw + 0.5*SAPS_DELAY))
delay(2.0*tau1 - 2.0*0.64*pw - SAPS_DELAY );
}
decrgpulse(pwC,three,0.0,0.0); /* flip transferred 13C-magn. to z */
decrgpulse(pwC,one,0.0,0.0); /* flip transferred 13C-magn. to z */
decphase(zero);
decpwrf(rfd);
starthardloop(v2);
decrgpulse(6.4*p_d2,zero,0.0,0.0);
decrgpulse(8.2*p_d2,two,0.0,0.0);
decrgpulse(5.8*p_d2,zero,0.0,0.0);
decrgpulse(5.7*p_d2,two,0.0,0.0);
decrgpulse(0.6*p_d2,zero,0.0,0.0);
decrgpulse(4.9*p_d2,two,0.0,0.0);
decrgpulse(7.5*p_d2,zero,0.0,0.0);
decrgpulse(5.3*p_d2,two,0.0,0.0);
decrgpulse(7.4*p_d2,zero,0.0,0.0);
decrgpulse(6.4*p_d2,two,0.0,0.0);
decrgpulse(8.2*p_d2,zero,0.0,0.0);
decrgpulse(5.8*p_d2,two,0.0,0.0);
decrgpulse(5.7*p_d2,zero,0.0,0.0);
decrgpulse(0.6*p_d2,two,0.0,0.0);
decrgpulse(4.9*p_d2,zero,0.0,0.0);
decrgpulse(7.5*p_d2,two,0.0,0.0);
decrgpulse(5.3*p_d2,zero,0.0,0.0);
decrgpulse(7.4*p_d2,two,0.0,0.0);
decrgpulse(6.4*p_d2,two,0.0,0.0);
decrgpulse(8.2*p_d2,zero,0.0,0.0);
decrgpulse(5.8*p_d2,two,0.0,0.0);
decrgpulse(5.7*p_d2,zero,0.0,0.0);
decrgpulse(0.6*p_d2,two,0.0,0.0);
decrgpulse(4.9*p_d2,zero,0.0,0.0);
decrgpulse(7.5*p_d2,two,0.0,0.0);
decrgpulse(5.3*p_d2,zero,0.0,0.0);
decrgpulse(7.4*p_d2,two,0.0,0.0);
decrgpulse(6.4*p_d2,zero,0.0,0.0);
decrgpulse(8.2*p_d2,two,0.0,0.0);
decrgpulse(5.8*p_d2,zero,0.0,0.0);
decrgpulse(5.7*p_d2,two,0.0,0.0);
decrgpulse(0.6*p_d2,zero,0.0,0.0);
decrgpulse(4.9*p_d2,two,0.0,0.0);
decrgpulse(7.5*p_d2,zero,0.0,0.0);
decrgpulse(5.3*p_d2,two,0.0,0.0);
decrgpulse(7.4*p_d2,zero,0.0,0.0);
endhardloop();
decphase(t3);
decpwrf(4095.0);
decrgpulse(pwC,three,0.0,0.0); /* flip transferred 13C-magnetization to z */
decoffset(dof_92p5); /* Preset the carbon frequency for the C1' carbon */
decrgpulse(pwC,t3,0.0,0.0); /* 4x,-4x flip transferred 13C-magnetization to x */
if (SE[A]=='y')
{
/***************** CONSTANT TIME EVOLUTION *****************/
if (CT[A]=='y') {
/***************/
initval(90.0, v9);
decstepsize(1.0);
dcplrphase(v9);
decphase(t9);
delay(CTdelay/2.0 - tau2);
decrgpulse(2.0*pwC, t9, 0.0, 0.0);
dcplrphase(zero);
decphase(t10);
if (tau2 < gt1 + gstab)
{delay(CTdelay/2.0 - pwZa - gt1 - gstab);
zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
delay(gstab - 2.0*GRADIENT_DELAY);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
delay(tau2);}
else {delay(CTdelay/2.0 - pwZa);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
delay(tau2 - gt1 - gstab);
zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
delay(gstab - 2.0*GRADIENT_DELAY);}
/***************/
}
/********************************************************************/
/***************** NORMAL EVOLUTION *****************/
else {
/***************/
if (CChomodec[A]=='y')
{
decpower(CCdpwr); decphase(zero);
decprgon(CCdseq,1.0/CCdmf,CCdres);
decon(); /* CC decoupling on */
}
decphase(zero);
delay(tau2);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
decphase(t9);
delay(gt1 + gstab - pwZa);
delay(tau2);
if (CChomodec[A]=='y')
{
decoff(); decprgoff(); /* CC decoupling off */
decpower(pwClvl);
}
decrgpulse(2.0*pwC, t9, 0.0, 0.0);
zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
decphase(t10);
delay(gstab - 2.0*GRADIENT_DELAY);
/***************/
}
/********************************************************************/
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
decrgpulse(pwC, zero, 0.0, 0.0);
decphase(zero);
zgradpulse(gzlvl5, gt5);
delay(lambda - 0.5*pwC - gt5);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
txphase(one);
decphase(t10);
delay(lambda - 0.5*pwC - gt5);
simpulse(pw, pwC, one, t10, 0.0, 0.0);
txphase(zero);
decphase(zero);
zgradpulse(gzlvl6, gt5);
delay(lambda - 0.5*pwC - gt5);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
txphase(two);
zgradpulse(gzlvl6, gt5);
delay(lambda - 0.5*pwC - gt5);
simpulse(pw, pwC, two, zero, 0.0, 0.0);
txphase(zero);
delay(lambda - 0.5*pwC);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
dcplrphase(zero); /* SAPS_DELAY */
zgradpulse(gzlvl2, gt1/4.0); /* 2.0*GRADIENT_DELAY */
delay(lambda - gt1/4.0 - 0.5*pwC - 2.0*GRADIENT_DELAY - 2*POWER_DELAY - SAPS_DELAY);
}
else
{
decphase(zero);
zgradpulse(gzlvl5,gt5);
delay(lambda - gt5);
simpulse(2*pw,2*pwC,zero,zero,0.0,0.0);
zgradpulse(gzlvl5,gt5);
delay(lambda - gt5);
decrgpulse(pwC,zero,0.0,0.0);
zgradpulse(gzlvl3,gt3);
delay(gstab);
obspower(tpwrs);
shaped_pulse("rna_H2Osinc", pwHs, zero, 5.0e-4, 0.0);
obspower(tpwr);
rgpulse(pw, zero, 2*rof1, 0.0);
txphase(two);
obspower(tpwrs2);
zgradpulse(gzlvl4,gt4);
delay(gstab - 2*SAPS_DELAY - 2*POWER_DELAY - GRADIENT_DELAY);
rgpulse((lambda-gstab-gt4-pwC), two, 0.0, 0.0);
simpulse(pwC,pwC,two,three,0.0,0.0);
simpulse(2*pwC,2*pwC,two,zero,0.0,0.0);
simpulse(pwC,pwC,two,three,0.0,0.0);
rgpulse((pwHs2-2*pwC-(lambda-gstab-gt4-pwC)), two, 0.0, 0.0);
txphase(zero);
obspower(tpwr);
rgpulse(2*pw, zero, 0.0, 0.0);
obspower(tpwrs2);
rgpulse(pwHs2, two, 4.0e-6, 0.0);
decphase(t4);
zgradpulse(gzlvl4,gt4);
delay(gstab-2*pwC-2*SAPS_DELAY - POWER_DELAY - GRADIENT_DELAY);
decrgpulse(pwC,t4,0.0,0.0);
decrgpulse(pwC,zero,0.0,0.0);
}
dec2power(dpwr2); /* 2*POWER_DELAY */
decpower(dpwr);
status(C);
setreceiver(t11);
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
CP hx = getcp("HX",0.0,0.0,0,1);
strncpy(hx.fr,"dec",3);
strncpy(hx.to,"obs",3);
putCmd("frHX='dec'\n");
putCmd("toHX='obs'\n");
MPSEQ c7 = getpostc7("c7X",0,0.0,0.0,0,1);
MPSEQ c7ref = getpostc7("c7X",c7.iSuper,c7.phAccum,c7.phInt,1,1);
strncpy(c7.ch,"obs",3);
putCmd("chXc7='obs'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
// Set Constant-time Period for d2.
if (d2_index == 0) d2_init = getval("d2");
double d2_ = (ni - 1)/sw1 + d2_init;
putCmd("d2acqret = %f\n",roundoff(d2_,12.5e-9));
putCmd("d2dwret = %f\n",roundoff(1.0/sw1,12.5e-9));
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwH90") + getval("tHX") + 2.0* getval("pwX90") + c7.t + c7ref.t;
d.dutyoff = d1 + 4.0e-6 + 2.0*getval("tZF");
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = d2_ + getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = d2_ + getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phH90,4,table1);
settable(phXhx,4,table2);
settable(phHhx,4,table3);
settable(phXmix1,4,table4);
settable(phXmix2,4,table5);
settable(phRec,4,table6);
// Add STATES-TPPI (STATES + "FAD")
double obsstep = 360.0/(PSD*8192);
if (phase1 == 2)
initval((45.0/obsstep),v1);
else
initval(0.0,v1);
initval((d2*c7.of[0]*360.0/obsstep),v2);
obsstepsize(obsstep);
setreceiver(phRec);
// Begin Sequence
txphase(phXhx); decphase(phH90);
obspwrf(getval("aXhx")); decpwrf(getval("aH90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H to X Cross Polarization
decrgpulse(getval("pwH90"),phH90,0.0,0.0);
decphase(phHhx);
_cp_(hx,phHhx,phXhx);
obspwrf(getval("aX90"));
// Mixing with C7 Recoupling-Period One
rgpulse(getval("pwX90"),phXmix1,0.0,0.0);
obspwrf(getval("aXc7"));
decoff();
xmtrphase(v1); txphase(phXmix1);
delay(getval("tZF"));
decpwrf(getval("aHmix"));
decunblank();
decon();
_mpseq(c7, phXmix1);
decoff();
// F1 Indirect Period For X
xmtrphase(v2); txphase(phXmix2);
_dseqon(dec);
delay(d2);
_dseqoff(dec);
// Mixing with C7 Recoupling-Period Two
decpwrf(getval("aHmix"));
decunblank();
decon();
_mpseq(c7ref, phXmix2);
decoff();
obspwrf(getval("aX90"));
xmtrphase(zero); txphase(phXmix2);
delay(getval("tZF"));
rgpulse(getval("pwX90"),phXmix2,0.0,0.0);
// Begin Acquisition
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence()
{
/* DECLARE AND LOAD VARIABLES */
char f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
mag_flg[MAXSTR], /* magic-angle coherence transfer gradients */
C13refoc[MAXSTR], /* C13 sech/tanh pulse in middle of t1*/
NH2only[MAXSTR], /* spectrum of only NH2 groups */
amino[MAXSTR], /* select amino nitrogens */
imino[MAXSTR], /* select imino nitrogens */
T1[MAXSTR], /* insert T1 relaxation delay */
T1rho[MAXSTR], /* insert T1rho relaxation delay */
T2[MAXSTR], /* insert T2 relaxation delay */
bottom[MAXSTR],
right[MAXSTR],
TROSY[MAXSTR]; /* do TROSY on N15 and H1 */
int icosel, /* used to get n and p type */
t1_counter, /* used for states tppi in t1 */
rTnum, /* number of relaxation times, relaxT */
rTcounter; /* to obtain maximum relaxT, ie relaxTmax */
double tau1, /* t1 delay */
lambda = 0.91/(4.0*getval("JNH")), /* 1/4J H1 evolution delay */
tNH = 1.0/(4.0*getval("JNH")), /* 1/4J N15 evolution delay */
relaxT = getval("relaxT"), /* total relaxation time */
rTarray[1000], /* to obtain maximum relaxT, ie relaxTmax */
maxrelaxT = getval("maxrelaxT"), /* maximum relaxT in all exps */
ncyc, /* number of pulsed cycles in relaxT */
/* the sech/tanh pulse is automatically calculated by the macro "rna_cal", */ /* and is called directly from your shapelib. */
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rfC, /* maximum fine power when using pwC pulses */
rfst, /* fine power for the rna_stC140 pulse */
/* temporary Pbox parameters */
bw, pws, ofs, ppm, nst, /* bandwidth, pulsewidth, offset, ppm, # steps */
compH = getval("compH"), /* adjustment for H1 amplifier compression */
compN = getval("compN"), /* adjustment for N15 amplifier compression */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
calH = getval("calH"), /* multiplier on a pw pulse for H1 calibration */
pwHs = getval("pwHs"), /* H1 90 degree pulse length at tpwrs */
tpwrs, /* power for the pwHs ("H2Osinc") pulse */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
calN = getval("calN"), /* multiplier on a pwN pulse for calibration */
slNlvl, /* power for N15 spin lock */
slNrf = 1500.0, /* RF field in Hz for N15 spin lock at 600 MHz */
dof2a, /* offset for imino/amino */
sw1 = getval("sw1"),
gt1 = getval("gt1"), /* coherence pathway gradients */
gzcal = getval("gzcal"), /* dac to G/cm conversion */
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
BPpwrlimits, /* =0 for no limit, =1 for limit */
gt0 = getval("gt0"), /* other gradients */
gt3 = getval("gt3"),
gt4 = getval("gt4"),
gt5 = getval("gt5"),
gzlvl0 = getval("gzlvl0"),
gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5");
P_getreal(GLOBAL,"BPpwrlimits",&BPpwrlimits,1);
getstr("f1180",f1180);
getstr("mag_flg",mag_flg);
getstr("C13refoc",C13refoc);
getstr("NH2only",NH2only);
getstr("T1",T1);
getstr("T1rho",T1rho);
getstr("T2",T2);
getstr("bottom",bottom);
getstr("right",right);
getstr("TROSY",TROSY);
getstr("imino",imino);
getstr("amino",amino);
/* LOAD PHASE TABLE */
settable(t3,2,phi3);
if (TROSY[A]=='y')
{ settable(t1,1,ph_x);
if (bottom[A]=='y')
settable(t4,1,phx);
else
settable(t4,1,ph_x);
if (right[A]=='y')
settable(t10,1,phy);
else
settable(t10,1,ph_y);
settable(t9,1,phx);
settable(t11,1,phx);
settable(t12,2,recT);
}
else
{ settable(t1,1,phx);
settable(t4,1,phx);
settable(t9,8,phi9);
settable(t10,1,phx);
settable(t11,1,phy);
settable(t12,4,rec);
}
/* INITIALIZE VARIABLES */
dof2a=dof2;
/* IMINO-region setting of dof2 */
if (imino[A] == 'y') dof2a=dof2-45*dfrq2;
if (amino[A] == 'y') dof2a=dof2-115*dfrq2;
if ((imino[A] == 'n') && (amino[A] == 'n')) dof2a=dof2;
/* maximum fine power for pwC pulses (and initialize rfst) */
rfC = 4095.0;
rfst=0.0;
setautocal(); /* activate auto-calibration flags */
if (autocal[0] == 'n')
{
/* 180 degree adiabatic C13 pulse covers 140 ppm */
if (C13refoc[A]=='y')
{ rfst = (compC*4095.0*pwC*4000.0*sqrt((21.0*sfrq/600.0+7.0)/0.35));
rfst = (int) (rfst + 0.5);
if ( 1.0/(4000.0*sqrt((21.0*sfrq/600.0+7.0)/0.35)) < pwC )
{ text_error( " Not enough C13 RF. pwC must be %f usec or less.\n",
(1.0e6/(4000.0*sqrt((21.0*sfrq/600.0+7.0)/0.35))) );
psg_abort(1);
}
}
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /*needs 1.69 times more*/
tpwrs = (int) (tpwrs); /*power than a square pulse */
}
else /* if autocal = 'y'(yes), 'q'(quiet), r(read), or 's'(semi) */
{
if(FIRST_FID) /* call Pbox */
{
ppm = getval("dfrq");
bw = 140.0*ppm;
pws = 0.001;
ofs = 0.0;
nst = 1000.0;
if (C13refoc[A]=='y')
stC140 = pbox_makeA("rna_stC140", "sech", bw, pws, ofs, compC*pwC, pwClvl, nst);
H2Osinc = pbox_Rsh("rna_H2Osinc", "sinc90", pwHs, 0.0, compH*pw, tpwr);
if (dm3[B] == 'y') H2ofs = 3.2;
ofs_check(H1ofs, C13ofs, N15ofs, H2ofs);
}
if (C13refoc[A]=='y') rfst = stC140.pwrf;
pwHs = H2Osinc.pw;
tpwrs = H2Osinc.pwr;
}
/* power level for N15 spinlock (90 degree pulse length calculated first) */
slNlvl = 1/(4.0*slNrf*sfrq/600.0) ;
slNlvl = pwNlvl - 20.0*log10(slNlvl/(pwN*compN));
slNlvl = (int) (slNlvl + 0.5);
/* use 1/8J times for relaxation measurements of NH2 groups */
if ( (NH2only[A]=='y') && ((T1[A]=='y') || (T1rho[A]=='y') || (T2[A]=='y')) )
{
tNH = tNH/2.0;
}
/* reset calH and calN for 2D if inadvertently left at 2.0 */
if (ni>1.0) {
calH=1.0;
calN=1.0;
}
/* CHECK VALIDITY OF PARAMETER RANGES */
if ( ((imino[A] == 'y') && (amino[A] == 'y')) )
{
printf(" Choose ONE of the cases: imino='y' OR amino='y' ");
psg_abort(1);
}
if ( ((imino[A] == 'y') && (NH2only[A] == 'y')) )
{
printf(" NH2only='y' only valide for amino='y' ");
psg_abort(1);
}
if ((TROSY[A]=='y') && (gt1 < -2.0e-4 + pwHs + 1.0e-4 + 2.0*POWER_DELAY))
{ text_error( " gt1 is too small. Make gt1 equal to %f or more.\n",
(-2.0e-4 + pwHs + 1.0e-4 + 2.0*POWER_DELAY) );
psg_abort(1);
}
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y' ))
{
text_error("incorrect dec1 decoupler flags! Should be 'nnn' ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y'))
{
text_error("incorrect dec2 decoupler flags! Should be 'nny' ");
psg_abort(1);
}
if( dpwr2 > 50 )
{
text_error("don't fry the probe, DPWR2 too large! ");
psg_abort(1);
}
if( pw > 50.0e-6 )
{
text_error("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( (pwN > 100.0e-6) && (pwNlvl > 54) )
{
text_error("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
/* RELAXATION TIMES AND FLAGS */
/* evaluate maximum relaxT, relaxTmax chosen by the user */
rTnum = getarray("relaxT", rTarray);
relaxTmax = rTarray[0];
for (rTcounter=1; rTcounter<rTnum; rTcounter++)
if (relaxTmax < rTarray[rTcounter]) relaxTmax = rTarray[rTcounter];
/* compare relaxTmax with maxrelaxT */
if (maxrelaxT > relaxTmax) relaxTmax = maxrelaxT;
if ( ((T1rho[A]=='y') || (T2[A]=='y')) && (relaxTmax > d1) )
{
text_error("Maximum relaxation time, relaxT, is greater than d1 ! ");
psg_abort(1);
}
if ( ((T1[A]=='y') && (T1rho[A]=='y')) || ((T1[A]=='y') && (T2[A]=='y')) ||
((T1rho[A]=='y') && (T2[A]=='y')) )
{
text_error("Choose only one relaxation measurement ! ");
psg_abort(1);
}
if ( ((T1[A]=='y') || (T1rho[A]=='y')) &&
((relaxT*100.0 - (int)(relaxT*100.0+1.0e-4)) > 1.0e-6) )
{
text_error("Relaxation time, relaxT, must be zero or multiple of 10msec");
psg_abort(1);
}
if ( (T2[A]=='y') &&
(((relaxT+0.01)*50.0 - (int)((relaxT+0.01)*50.0+1.0e-4)) > 1.0e-6) )
{
text_error("Relaxation time, relaxT, must be odd multiple of 10msec");
psg_abort(1);
}
if ( ((T1rho[A]=='y') || (T2[A]=='y')) && (relaxTmax > 0.25) && (ix==1) )
{
printf("WARNING, sample heating may result in a reduced lock level for relaxT>0.25sec");
}
if ( ((T1rho[A]=='y') || (T2[A]=='y')) && (relaxTmax > 0.5) )
{
text_error("relaxT greater than 0.5 seconds will heat sample");
psg_abort(1);
}
if ( ((NH2only[A]=='y') || (T1[A]=='y') || (T1rho[A]=='y') || (T2[A]=='y'))
&& (TROSY[A]=='y') )
{
text_error("TROSY not implemented with NH2 spectrum, or relaxation exps.");
psg_abort(1);
}
if ((TROSY[A]=='y') && (dm2[C] == 'y'))
{
text_error("Choose either TROSY='n' or dm2='nnn' ! ");
psg_abort(1);
}
/* PHASES AND INCREMENTED TIMES */
/* Phase incrementation for hypercomplex 2D data, States-Haberkorn element */
if (TROSY[A]=='y')
{ if (phase1 == 1) icosel = -1;
else {
tsadd(t4,2,4);
tsadd(t10,2,4);
icosel = +1;
}
}
else {
if (phase1 == 1) {
tsadd(t10,2,4);
icosel = +1;
}
else icosel = -1;
}
/* Set up f1180 */
tau1 = d2;
if((f1180[A] == 'y') && (ni > 1.0))
{
tau1 += ( 1.0 / (2.0*sw1) );
if(tau1 < 0.2e-6) tau1 = 0.0;
}
tau1 = tau1/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{
tsadd(t3,2,4);
tsadd(t12,2,4);
}
/* Correct inverted signals for NH2 only spectra */
if ((NH2only[A]=='y') && (T1[A]=='n') && (T1rho[A]=='n') && (T2[A]=='n'))
{
tsadd(t3,2,4);
}
/* BEGIN PULSE SEQUENCE */
status(A);
obspower(tpwr);
decpower(pwClvl);
decpwrf(rfC);
dec2power(pwNlvl);
dec2offset(dof2a);
txphase(zero);
decphase(zero);
dec2phase(zero);
delay(d1);
/* xxxxxxxxxxxxxxxxx CONSTANT SAMPLE HEATING FROM N15 RF xxxxxxxxxxxxxxxxx */
if (T1rho[A]=='y')
{ dec2power(slNlvl);
dec2rgpulse(relaxTmax-relaxT, zero, 0.0, 0.0);
dec2power(pwNlvl);
}
if (T2[A]=='y')
{ ncyc = 8.0*100.0*(relaxTmax - relaxT);
if (BPpwrlimits > 0.5)
{
dec2power(pwNlvl-3.0); /* reduce for probe protection */
pwN=pwN*compN*1.4;
}
if (ncyc > 0)
{ initval(ncyc,v1);
loop(v1,v2);
delay(0.625e-3 - pwN);
dec2rgpulse(2*pwN, zero, 0.0, 0.0);
delay(0.625e-3 - pwN);
endloop(v2);
}
if (BPpwrlimits > 0.5)
{
dec2power(pwNlvl); /* restore normal value */
pwN=getval("pwN");
}
}
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
rcvroff();
if (TROSY[A]=='n') dec2rgpulse(pwN, zero, 0.0, 0.0);
decrgpulse(pwC, zero, 0.0, 0.0); /*destroy N15 and C13 magnetization*/
zgradpulse(gzlvl0, 0.5e-3);
delay(1.0e-4);
if (TROSY[A]=='n') dec2rgpulse(pwN, one, 0.0, 0.0);
decrgpulse(pwC, one, 0.0, 0.0);
zgradpulse(0.7*gzlvl0, 0.5e-3);
decpwrf(rfst);
txphase(t1);
delay(5.0e-4);
if(dm3[B] == 'y') /*optional 2H decoupling on */
{ lk_hold();
dec3unblank();
dec3rgpulse(1/dmf3, one, 0.0, 0.0);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
}
rgpulse(calH*pw,t1,0.0,0.0); /* 1H pulse excitation */
txphase(zero);
dec2phase(zero);
zgradpulse(gzlvl0, gt0);
delay(lambda - gt0);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
txphase(one);
zgradpulse(gzlvl0, gt0);
delay(lambda - gt0);
rgpulse(pw, one, 0.0, 0.0);
txphase(two);
obspower(tpwrs);
shaped_pulse("rna_H2Osinc", pwHs, two, 5.0e-5, 0.0);
obspower(tpwr);
zgradpulse(gzlvl3, gt3);
dec2phase(t3);
delay(2.0e-4);
dec2rgpulse(calN*pwN, t3, 0.0, 0.0);
txphase(zero);
decphase(zero);
/* xxxxxxxxxxxxxxxxxx OPTIONS FOR N15 RELAXATION xxxxxxxxxxxxxxxxxxxx */
if ( (T1[A]=='y') || (T1rho[A]=='y') || (T2[A]=='y') )
{
dec2phase(one);
zgradpulse(gzlvl4, gt4); /* 2.0*GRADIENT_DELAY */
delay(tNH - gt4 - 2.0*GRADIENT_DELAY);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, one, 0.0, 0.0);
zgradpulse(gzlvl4, gt4); /* 2.0*GRADIENT_DELAY */
delay(tNH - gt4 - 2.0*GRADIENT_DELAY);
}
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
if (T1[A]=='y')
{
dec2rgpulse(pwN, one, 0.0, 0.0);
dec2phase(three);
zgradpulse(gzlvl0, gt0); /* 2.0*GRADIENT_DELAY */
delay(2.5e-3 - gt0 - 2.0*GRADIENT_DELAY - pw);
rgpulse(2.0*pw, zero, 0.0, 0.0);
delay(2.5e-3 - pw);
ncyc = (100.0*relaxT);
initval(ncyc,v4);
if (ncyc > 0)
{ loop(v4,v5);
delay(2.5e-3 - pw);
rgpulse(2.0*pw, two, 0.0, 0.0);
delay(2.5e-3 - pw);
delay(2.5e-3 - pw);
rgpulse(2.0*pw, zero, 0.0, 0.0);
delay(2.5e-3 - pw);
endloop(v5);
}
dec2rgpulse(pwN, three, 0.0, 0.0);
}
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
/* Theory suggests 8.0 is better than 2PI as RF */
/* field multiplier and experiment confirms this.*/
if (T1rho[A]=='y') /* Shift evolution of 2.0*pwN/PI for one pulse */
{ /* at end left unrefocused as for normal sequence*/
delay(1.0/(8.0*slNrf) - pwN);
decrgpulse(pwN, zero, 0.0, 0.0);
dec2power(slNlvl);
/* minimum 5ms spinlock to dephase */
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0); /* spins not locked */
sim3pulse(2.0*pw, 0.0, 2.0*pw, zero, zero, zero, 0.0, 0.0);
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0);
ncyc = 100.0*relaxT;
initval(ncyc,v4);
if (ncyc > 0)
{ loop(v4,v5);
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0);
sim3pulse(2.0*pw, 0.0, 2.0*pw, two, zero, zero, 0.0, 0.0);
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0);
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0);
sim3pulse(2.0*pw, 0.0, 2.0*pw, zero, zero, zero, 0.0, 0.0);
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0);
endloop(v5);
}
dec2power(pwNlvl);
decrgpulse(pwN, zero, 0.0, 0.0);
delay(1.0/(8.0*slNrf) + 2.0*pwN/PI - pwN);
}
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
if (T2[A]=='y')
{
dec2phase(zero);
initval(0.0,v3);
initval(180.0,v4);
if (BPpwrlimits > 0.5)
{
dec2power(pwNlvl-3.0); /* reduce for probe protection */
pwN=pwN*compN*1.4;
}
ncyc = 100.0*relaxT;
initval(ncyc,v5);
loop(v5,v6);
initval(3.0,v7);
loop(v7,v8);
delay(0.625e-3 - pwN);
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
delay(0.625e-3 - pwN);
endloop(v8);
delay(0.625e-3 - pwN - SAPS_DELAY);
add(v4,v3,v3);
obsstepsize(1.0);
xmtrphase(v3); /* SAPS_DELAY */
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
delay(0.625e-3 - pwN - pw);
rgpulse(2*pw, zero, 0.0, 0.0);
delay(0.625e-3 - pwN - pw );
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
xmtrphase(zero); /* SAPS_DELAY */
delay(0.625e-3 - pwN - SAPS_DELAY);
initval(3.0,v9);
loop(v9,v10);
delay(0.625e-3 - pwN);
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
delay(0.625e-3 - pwN);
endloop(v10);
endloop(v6);
if (BPpwrlimits > 0.5)
{
dec2power(pwNlvl); /* restore normal value */
pwN=getval("pwN");
}
}
/* xxxxxxxxxxxxxxxxxx OPTIONS FOR N15 EVOLUTION xxxxxxxxxxxxxxxxxxxxx */
txphase(zero);
dec2phase(t9);
if ( (NH2only[A]=='y') || (T1[A]=='y') || (T1rho[A]=='y') || (T2[A]=='y') )
{
delay(tau1);
/* optional sech/tanh pulse in middle of t1 */
if (C13refoc[A]=='y') /* WFG_START_DELAY */
{ decshaped_pulse("rna_stC140", 1.0e-3, zero, 0.0, 0.0);
delay(tNH - 1.0e-3 - WFG_START_DELAY - 2.0*pw);
}
else
{
delay(tNH - 2.0*pw);
}
rgpulse(2.0*pw, zero, 0.0, 0.0);
if (tNH < gt1 + 1.99e-4) delay(gt1 + 1.99e-4 - tNH);
delay(tau1);
dec2rgpulse(2.0*pwN, t9, 0.0, 0.0);
if (mag_flg[A] == 'y') magradpulse(gzcal*gzlvl1, gt1);
else zgradpulse(gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
txphase(t4);
dec2phase(t10);
if (tNH > gt1 + 1.99e-4) delay(tNH - gt1 - 2.0*GRADIENT_DELAY);
else delay(1.99e-4 - 2.0*GRADIENT_DELAY);
}
else if (TROSY[A]=='y')
{
if ( (C13refoc[A]=='y') && (tau1 > 0.5e-3 + WFG2_START_DELAY) )
{ delay(tau1 - 0.5e-3 - WFG2_START_DELAY); /* WFG2_START_DELAY */
decshaped_pulse("rna_stC140", 1.0e-3, zero, 0.0, 0.0);
delay(tau1 - 0.5e-3);
}
else delay(2.0*tau1);
if (mag_flg[A] == 'y') magradpulse(gzcal*gzlvl1, gt1);
else zgradpulse(gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
delay(2.0e-4 - 2.0*GRADIENT_DELAY);
dec2rgpulse(2.0*pwN, t9, 0.0, 0.0);
delay(gt1 + 2.0e-4 - pwHs - 1.0e-4 - 2.0*POWER_DELAY);
txphase(three);
obspower(tpwrs); /* POWER_DELAY */
shaped_pulse("rna_H2Osinc", pwHs, three, 5.0e-5, 0.0);
txphase(t4);
obspower(tpwr); /* POWER_DELAY */
delay(5.0e-5);
}
else
{ /* fully-coupled spectrum */
if (dm2[C]=='n') {
rgpulse(2.0*pw, zero, 0.0, 0.0);
pw=0.0;
}
if ( (C13refoc[A]=='y') && (tau1 > 0.5e-3 + WFG2_START_DELAY) )
{ delay(tau1 - 0.5e-3 - WFG2_START_DELAY); /* WFG2_START_DELAY */
simshaped_pulse("", "rna_stC140", 2.0*pw, 1.0e-3, zero, zero, 0.0, 0.0);
delay(tau1 - 0.5e-3);
delay(gt1 + 2.0e-4);
}
else
{ delay(tau1);
rgpulse(2.0*pw, zero, 0.0, 0.0);
delay(gt1 + 2.0e-4 - 2.0*pw);
delay(tau1);
}
decphase(zero);
pw=getval("pw");
dec2rgpulse(2.0*pwN, t9, 0.0, 0.0);
if (mag_flg[A] == 'y') magradpulse(gzcal*gzlvl1, gt1);
else zgradpulse(gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
txphase(t4);
dec2phase(t10);
delay(2.0e-4 - 2.0*GRADIENT_DELAY);
}
if (T1rho[A]=='y') delay(POWER_DELAY);
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
if (TROSY[A]=='y') rgpulse(pw, t4, 0.0, 0.0);
else sim3pulse(pw, 0.0, pwN, t4, zero, t10, 0.0, 0.0);
txphase(zero);
dec2phase(zero);
zgradpulse(gzlvl5, gt5);
if (TROSY[A]=='y') delay(lambda - 0.65*(pw + pwN) - gt5);
else delay(lambda - 1.3*pwN - gt5);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
txphase(one);
dec2phase(t11);
delay(lambda - 1.3*pwN - gt5);
sim3pulse(pw, 0.0, pwN, one, zero, t11, 0.0, 0.0);
txphase(zero);
dec2phase(zero);
zgradpulse(1.5*gzlvl5, gt5);
delay(lambda - 1.3*pwN - gt5);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
dec2phase(t10);
zgradpulse(1.5*gzlvl5, gt5);
if (TROSY[A]=='y') delay(lambda - 1.6*pwN - gt5);
else delay(lambda - 0.65*pwN - gt5);
if (TROSY[A]=='y') dec2rgpulse(pwN, t10, 0.0, 0.0);
else rgpulse(pw, zero, 0.0, 0.0);
delay((gt1/10.0) + 1.0e-4 - 0.65*pw + 2.0*GRADIENT_DELAY + POWER_DELAY);
if (dm3[B] == 'y') /*optional 2H decoupling off */
{ dec3rgpulse(1/dmf3, three, 0.0, 0.0);
dec3blank();
setstatus(DEC3ch, FALSE, 'w', FALSE, dmf3);
dec3blank();
}
rgpulse(2.0*pw, zero, 0.0, rof1);
dec2power(dpwr2); /* POWER_DELAY */
if (mag_flg[A] == 'y') magradpulse(icosel*gzcal*gzlvl2, 0.1*gt1);
else zgradpulse(icosel*gzlvl2, 0.1*gt1); /* 2.0*GRADIENT_DELAY */
rcvron();
statusdelay(C,1.0e-4-rof1);
if (dm3[B] == 'y') {
delay(1/dmf3);
lk_sample();
}
setreceiver(t12);
}
pulsesequence()
{
double gzlvl1 = getval("gzlvl1"),
gt1 = getval("gt1"),
gstab = getval("gstab"),
del = getval("del"),
tweek = getval("tweek"),
prgtime = getval("prgtime"),
prgpwr = getval("prgpwr"),
satpwr = getval("satpwr"),
satdly = getval("satdly"),
gzlvl2 = getval("gzlvl2"),
gt2 = getval("gt2"),
gzlvlhs = getval("gzlvlhs"),
hsgt = getval("hsgt"),
wrefpwr = getval("wrefpwr"),
wrefpw = getval("wrefpw"),
wrefpwrf = getval("wrefpwrf"),
dosytimecubed,dosyfrq,Ddelta;
char delflag[MAXSTR],lkgate_flg[MAXSTR],alt_grd[MAXSTR],prg_flg[MAXSTR];
char satmode[MAXSTR],sspul[MAXSTR],wrefshape[MAXSTR];
getstr("delflag",delflag);
getstr("lkgate_flg",lkgate_flg);
getstr("alt_grd",alt_grd);
getstr("prg_flg",prg_flg);
getstr("satmode",satmode);
getstr("sspul",sspul);
getstr("wrefshape", wrefshape);
//synchronize gradients to srate for probetype='nano'
// Preserve gradient "area"
gt1 = syncGradTime("gt1","gzlvl1",1.0);
gzlvl1 = syncGradLvl("gt1","gzlvl1",1.0);
gt2 = syncGradTime("gt2","gzlvl2",1.0);
gzlvl2 = syncGradLvl("gt2","gzlvl2",1.0);
if (del<(3*gt1+3.0*gstab+2.0*rof1+pw*2.0))
{ abort_message("DgcsteSL error: del is less than %f, too short!",
(3*gt1+3.0*gstab+2.0*rof1+pw*2.0));
}
/* Safety check for the duration of the purge pulse */
if (prgtime > 4.0e-2)
{ text_error("prgtime has been reset to a maximum of 2 ms");
prgtime = 2.0e-3;
}
dosyfrq=sfrq;
Ddelta=gt1; /* the diffusion-encoding pulse width is gt1 */
dosytimecubed=Ddelta*Ddelta*(del-(Ddelta/3.0));
putCmd("makedosyparams(%e,%e)\n",dosytimecubed,dosyfrq);
/* phase cycling calculation */
if (delflag[0]=='y')
{ hlv(ct,v4);
mod2(v4,v2);
mod2(ct,v1);
dbl(v1,v1);
add(v2,v1,v1);
mod4(v1,v1); /* 0 2 1 3 , 1st 90 */
hlv(v4,v4);
mod2(v4,v3);
dbl(v3,v3); /* (0)4 (2)4 */
hlv(v4,v4);
mod2(v4,v2);
dbl(v2,v2); /* (0)8 (2)8 */
hlv(v4,v4);
mod2(v4,v5);
add(v5,v3,v3);
mod4(v3,v3); /* {(0)4 (2)4}2 {(1)4 (3)4}2, 3rd 90 */
hlv(v4,v4);
assign(v4,v6);
mod2(v4,v4);
add(v4,v2,v2);
mod4(v2,v2); /* {(0)8 (2)8}2 {(1)8 (3)8}2, 2nd 90 */
mod2(ct,v10); /* gradients change sign every increment */
assign(v3,oph);
add(oph,v2,oph);
sub(oph,v1,oph);
mod4(oph,oph);
assign(oph,v5);
add(one,v5,v5);
mod2(ct,v7);
dbl(v7,v7);
hlv(v6,v6);
mod2(v6,v6);
dbl(v6,v6);
add(v7,v5,v5); /* the purge pulse is 90 deg. off the receiver phase */
add(v6,v5,v5); /* it is phase alternated every increment */
mod4(v5,v5); /* and also every 64 increments */
}
else
{
assign(ct,v4);
assign(oph,v4); /*v4 used only for normal s2pul- type sequence */
}
add(v3,two,v9);
if (ni > 1.0)
{ abort_message("DgcsteSL is a 2D, not a 3D dosy sequence: please set ni to 0 or 1");
}
/* equilibrium period */
status(A);
obspower(tpwr);
if (sspul[A] == 'y')
{
zgradpulse(gzlvlhs,hsgt);
rgpulse(pw,zero,rof1,rof1);
zgradpulse(gzlvlhs,hsgt);
}
if (satmode[0] == 'y')
{
if (d1 - satdly > 0)
delay(d1 - satdly);
else delay(0.02);
obspower(satpwr);
txphase(v1);
rgpulse(satdly,zero,rof1,rof1);
obspower(tpwr);
delay(1.0e-5);
}
else
{ delay(d1); }
obspower(tpwr);
status(B);
/* first part of bppdel sequence */
if(delflag[0] == 'y')
{ rgpulse(pw, v1, rof1, 0.0); /* first 90, v1 */
if (lkgate_flg[0] == 'y') lk_hold(); /* turn lock sampling off */
if (alt_grd[0] == 'y')
{ ifzero(v10);
zgradpulse(gzlvl1,gt1);
elsenz(v10);
zgradpulse(-1.0*gzlvl1,gt1);
endif(v10);
}
else
zgradpulse(gzlvl1,gt1);
delay(gstab);
rgpulse(pw, v2, rof1, 0.0); /* second 90, v2 */
if (alt_grd[0] == 'y')
{ ifzero(v10); /* compensating AND CPS gradient */
zgradpulse(-1.0*gzlvl1*(1.0-tweek),gt1);
elsenz(v10);
zgradpulse(gzlvl1*(1.0-tweek),gt1);
endif(v10);
}
else /* compensating AND CPS gradient */
zgradpulse(-1.0*gzlvl1*(1.0-tweek),gt1);
delay(gstab);
if (satmode[B] == 'y')
{ obspower(satpwr);
rgpulse(del-3.0*(gt1+gstab)-2.0*rof1-pw*2.0,zero,rof1,rof1);
obspower(tpwr);
}
else
delay(del-3.0*(gt1+gstab)-2.0*rof1-pw*2.0); /* diffusion delay */
if (alt_grd[0] == 'y')
{
ifzero(v10);
zgradpulse(-1.0*gzlvl1*(1.0+tweek),gt1);
elsenz(v10);
zgradpulse(gzlvl1*(1.0+tweek),gt1);
endif(v10);
}
else
zgradpulse(-1.0*gzlvl1*(1.0+tweek),gt1);
delay(gstab);
rgpulse(pw, v3, rof1, 0.0); /* third 90, v3 */
if (alt_grd[0] == 'y')
{
ifzero(v10);
zgradpulse(gzlvl1,gt1);
elsenz(v10);
zgradpulse(-1.0*gzlvl1,gt1);
endif(v10);
}
else
zgradpulse(gzlvl1,gt1);
delay(gstab);
if (alt_grd[0] == 'y')
{ ifzero(v10); zgradpulse(gzlvl2,gt2);
elsenz(v10); zgradpulse(-gzlvl2,gt2); endif(v10);}
else zgradpulse(gzlvl2,gt2);
obspower(wrefpwr+6); obspwrf(wrefpwrf);
delay(gstab);
shaped_pulse(wrefshape,wrefpw,v9,rof1,0.0);
obspower(tpwr); obspwrf(4095.0);
rgpulse(2.0*pw,v3,rof1,0.0);
if (alt_grd[0] == 'y')
{ ifzero(v10); zgradpulse(gzlvl2,gt2);
elsenz(v10); zgradpulse(-gzlvl2,gt2); endif(v10);}
else zgradpulse(gzlvl2,gt2);
obspower(wrefpwr+6); obspwrf(wrefpwrf);
delay(gstab);
if (alt_grd[0] == 'y')
{ ifzero(v10); zgradpulse(1.2*gzlvl2,gt2);
elsenz(v10); zgradpulse(-1.2*gzlvl2,gt2); endif(v10); }
else zgradpulse(1.2*gzlvl2,gt2);
delay(gstab);
shaped_pulse(wrefshape,wrefpw,v9,rof1,0.0);
obspower(tpwr); obspwrf(4095.0);
rgpulse(2.0*pw,v3,rof1,rof1);
if (alt_grd[0] == 'y')
{ ifzero(v10); zgradpulse(1.2*gzlvl2,gt2);
elsenz(v10); zgradpulse(-1.2*gzlvl2,gt2); endif(v10); }
else zgradpulse(1.2*gzlvl2,gt2);
delay(gstab+2.0*pw/3.14);
if (lkgate_flg[0] == 'y') lk_sample(); /* turn lock sampling on */
/* purge pulse to scramble any magnetisation that
is not in phase with the receiver */
if (prg_flg[0] == 'y')
{ obspower(prgpwr);
rgpulse(prgtime, v5, rof1, 0.0);
}
}
else
rgpulse(pw, v4, rof1, rof2); /* first 90, v1 */
status(C);
}
pulsesequence()
{
/* DECLARE VARIABLES */
char
satmode[MAXSTR],
f1180[MAXSTR],
abfilter[MAXSTR]; /* set 'a' for inphase and 'b' for antiphase */
int t1_counter,icosel,first_FID;
double /* DELAYS */
tau1, /* t1/2 */
tauhn,taunco,taucoca,taunca,tauhaca,taucaha,taucacb,
/* COUPLINGS */
jhn = getval("jhn"),
jnco = getval("jnco"),
jcoca = getval("jcoca"),
jnca = getval("jnca"),
jhaca = getval("jhaca"),
jcaha = getval("jcaha"),
jcacb = getval("jcacb"),
/* PULSES */
pwN = getval("pwN"), /* PW90 for N-nuc */
pwC = getval("pwC"), /* PW90 for C-nuc */
pwHs = getval("pwHs"),
/* POWER LEVELS */
satpwr = getval("satpwr"), /* low power level for presat */
tpwrsf_d = getval("tpwrsf_d"), /* fine power level for first pwHs */
tpwrsf_u = getval("tpwrsf_u"), /* fine power level for second pwHs */
tpwrs,
pwClvl = getval("pwClvl"), /* power level for C hard pulses */
compC = getval("compC"), /* amplifier compression factor */
compH = getval("compH"), /* amplifier compression factor */
pwNlvl = getval("pwNlvl"), /* power level for N hard pulses */
rf90onco,pw90onco, /* power level/pw for CO 90 pulses */
rf180onco,pw180onco, /* power level/pw for CO 180 pulses */
rf180offca,pw180offca, /* power level/pw for CA 180 pulses */
/* CONSTANTS */
kappa,
lambda = getval("lambda"), /* scaling factor for JNCa */
/* GRADIENT DELAYS AND LEVELS */
gt0 = getval("gt0"), /* gradient time */
gt1 = getval("gt1"), /* gradient time */
gt3 = getval("gt3"), /* gradient time */
gt5 = getval("gt5"),
gstab = getval("gstab"),
gzlvl0 = getval("gzlvl0"), /* level of gradient */
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gzlvl3 = getval("gzlvl3"),
gzlvl5 = getval("gzlvl5");
/* LOAD VARIABLES */
getstr("satmode",satmode);
getstr("f1180",f1180);
getstr("abfilter",abfilter);
/* check validity of parameter range */
if ((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y' ))
{
printf("incorrect Dec1 decoupler flags! ");
psg_abort(1);
}
if ((dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y' ))
{
printf("incorrect Dec2 decoupler flags! ");
psg_abort(1);
}
if ( satpwr > 8 )
{
printf("satpwr too large !!! ");
psg_abort(1);
}
if ( dpwr > 50 )
{
printf("don't fry the probe, dpwr too large! ");
psg_abort(1);
}
if ( dpwr2 > 50 )
{
printf("don't fry the probe, dpwr2 too large! ");
psg_abort(1);
}
/* LOAD VARIABLES */
settable(t1, 1, phi1);
settable(t2, 1, phi2);
settable(t3, 1, phi3);
settable(t4, 2, phi4);
settable(t6, 4, phi6);
settable(t7, 2, phi7);
/* INITIALIZE VARIABLES AND POWER LEVELS FOR PULSES */
tauhn = ((jhn != 0.0) ? 1/(4*(jhn)) : 2.25e-3);
taunco = ((jnco !=0.0) ? 1/(4*(jnco)) : 16.6e-3);
taucoca = ((jcoca !=0.0) ? 1/(4*(jcoca)) : 4.5e-3);
taunca = ((jnca !=0.0) ? 1/(4*(jnca)) : 12e-3);
tauhaca = ((jhaca !=0.0) ? 1/(4*(jhaca)) : 12e-3);
taucaha = ((jcaha !=0.0) ? 1/(4*(jcaha)) : 12e-3);
taucacb = ((jcacb !=0.0) ? 1/(4*(jcacb)) : 12e-3);
if((getval("arraydim") < 1.5) || (ix==1))
first_FID = 1;
else
first_FID = 0;
/* 90 degree pulse on CO, null at Ca 118ppm away */
pw90onco = sqrt(15.0)/(4.0*118.0*dfrq);
rf90onco = (4095.0*pwC*compC)/pw90onco;
rf90onco = (int) (rf90onco + 0.5);
if(rf90onco > 4095.0)
{
if(first_FID)
printf("insufficient power for pw90onco -> rf90onco (%.0f)\n", rf90onco);
rf90onco = 4095.0;
pw90onco = pwC;
}
/* 180 degree pulse on CO, null at Ca 118ppm away */
pw180onco = sqrt(3.0)/(2.0*118.0*dfrq);
rf180onco = (4095.0*pwC*compC*2.0)/pw180onco;
rf180onco = (int) (rf180onco + 0.5);
if(rf180onco > 4095.0)
{
if(first_FID)
printf("insufficient power for pw180onco -> rf180onco (%.0f)\n", rf180onco);
rf180onco = 4095.0;
pw180onco = pwC*2.0;
}
pw180offca = pw180onco; rf180offca = rf180onco;
/* Phase incrementation for hypercomplex data */
kappa=(taunco - tauhn - gt1 - gstab)/(0.5*ni/sw1)-0.001;
if (kappa > 1.0)
{
kappa=1.0-0.01;
}
if ( phase1 == 1) /* Hypercomplex in t2 */
{
icosel = -1;
tsadd(t2, 2, 4);
tsadd(t3, 2, 4);
}
else icosel = 1;
if (ix == 1)
printf("semi constant-time factor %4.6f\n",kappa);
/* calculate modification to phases based on current t1 values
to achieve States-TPPI acquisition */
if (ix == 1)
d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5);
if (t1_counter %2) /* STATES-TPPI */
{
tsadd(t1,2,4);
tsadd(t7,2,4);
}
/* set up so that get (-90,180) phase corrects in F1 if f1180 flag is y */
tau1 = d2;
if (f1180[A] == 'y') tau1 += ( 1.0/(2.0*sw1));
tau1 = tau1/2.0;
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /*needs 1.69 times more*/
tpwrs = (int) (tpwrs); /*power than a square pulse */
if (tpwrsf_d<4095.0) tpwrs=tpwrs+6.0; /* allow for fine power control via tpwrsf_d */
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(satpwr); /* Set power for presaturation */
decpower(pwClvl); /* Set decoupler1 power to pwClvl */
decpwrf(rf90onco);
dec2power(pwNlvl); /* Set decoupler2 power to pwNlvl */
/* Presaturation Period */
if (satmode[0] == 'y')
{
rgpulse(d1,zero,rof1,rof1);
obspower(tpwr); /* Set power for hard pulses */
}
else
{
obspower(tpwr); /* Set power for hard pulses */
delay(d1);
}
status(B);
rcvroff();
/* eliminate all magnetization originating on 13C */
decpwrf(rf90onco);
decrgpulse(pw90onco,zero,0.0,0.0);
zgradpulse(gzlvl0,gt0);
delay(gstab);
/* shaped pulse for water flip-back */
obspower(tpwrs); obspwrf(tpwrsf_d);
shaped_pulse("H2Osinc_d",pwHs,one,rof1,0.0);
delay(2.0e-6);
obspower(tpwr); obspwrf(4095.0);
/* shaped pulse */
/* transfer from HN to N by INEPT */
rgpulse(pw,zero,rof1,0.0); /* 90 for 1H */
zgradpulse(gzlvl0*1.3,gt0);
delay(gstab);
delay(tauhn - gt0 - gstab); /* 1/4JHN */
sim3pulse(2.0*pw,0.0,2.0*pwN,zero,zero,zero,0.0,0.0); /* 180 for 1H and 15N */
delay(tauhn - gt0 - gstab); /* 1/4JHN */
zgradpulse(gzlvl0*1.3,gt0);
delay(gstab);
rgpulse(pw,three,rof1,0.0);
zgradpulse(gzlvl3,gt3);
delay(gstab);
/* start transfer from N to CO */
dec2rgpulse(pwN,zero,0.0,0.0);
delay(taunco - gt0 - gstab - POWER_DELAY - 0.5*pw180onco); /* 1/4J(NCO) */
decpwrf(rf180onco); /* Set decoupler1 power to rf180onco */
zgradpulse(gzlvl0,gt0);
delay(gstab);
sim3pulse(0.0,pw180onco,2.0*pwN,zero,zero,zero,rof1,rof1); /* 180 for 13C' and 15N */
zgradpulse(gzlvl0,gt0);
delay(gstab);
decpwrf(rf90onco); /* Set decoupler1 power to rf90onco */
delay(taunco - gt0 - gstab - POWER_DELAY - 0.5*pw180onco); /* 1/4J(NCO) */
dec2rgpulse(pwN,one,0.0,0.0); /* 90 for 15N */
zgradpulse(gzlvl3*1.1,gt3);
delay(gstab);
decphase(t4);
decrgpulse(pw90onco,t4,0.0,0.0); /* 90 for 13C' */
if (abfilter[0] == 'b')
{
decpwrf(rf180offca); /* Set decoupler1 power to rf180offca */
decshaped_pulse("offC3",pw180offca,zero,0.0,0.0);
decpwrf(rf180onco); /* Set decoupler1 power to rf180onco */
delay(taucoca - gt0 - gstab - 0.5*pw180onco - pw180offca - 2.0*POWER_DELAY); /* 1/4J(COCA) */
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
decrgpulse(pw180onco,zero,0.0,0.0); /* 180 13C' */
decpwrf(rf180offca); /* Set decoupler1 power to rf180offca */
decshaped_pulse("offC3",pw180offca,zero,0.0,0.0);
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
delay(taucoca - gt0 - gstab - 0.5*pw180onco - pw180offca - 2.0*POWER_DELAY); /* 1/4J(NCO) */
decpwrf(rf90onco); /* Set decoupler1 power to rf90onco */
decrgpulse(pw90onco,one,0.0,0.0); /* 90 for 13C' do not touch alpha's */
/* Field crusher gradient */
zgradpulse(gzlvl3*0.9,gt3);
delay(gstab);
/* Field crusher gradient */
}
if (abfilter[0] == 'a')
{
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
decpwrf(rf180offca); /* Set decoupler1 power to rf180offca */
delay(0.5*taucoca - gt0 - gstab - POWER_DELAY - 0.5*pw180offca); /* 1/8J(COCA) */
decshaped_pulse("offC3",pw180offca,zero,0.0,0.0);
delay(0.5*taucoca - gt0 - gstab - POWER_DELAY - 0.5*pw180offca - 0.5*pw180onco); /* 1/8J(COCA) */
decpwrf(rf180onco); /* Set decoupler1 power to rf180onco */
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
decrgpulse(pw180onco,zero,0.0,0.0); /* 180 13C' */
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
decpwrf(rf180offca);
delay(0.5*taucoca - gt0 - gstab - POWER_DELAY - 0.5*pw180onco - 0.5*pw180offca); /* 1/8J(COCA) */
decshaped_pulse("offC3",pw180offca,zero,0.0,0.0);
delay(0.5*taucoca - gt0 - gstab - POWER_DELAY - 0.5*pw180offca); /* 1/8J(COCA) */
decpwrf(rf90onco); /* Set decoupler1 power to rf90onco */
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
decrgpulse(pw90onco,zero,0.0,0.0); /* 90 for 13C' */
/* Field crusher gradient */
zgradpulse(gzlvl3*0.9,gt3);
delay(gstab);
/* Field crusher gradient */
}
dec2rgpulse(pwN,t1,0.0,0.0); /* 90 15N */
if (lambda>0.0)
{
delay(lambda*tau1); /* (lambda)t1/2 */
decpwrf(rf180offca);
sim3shaped_pulse("","offC3","",0.0,pw180offca,2.0*pwN,zero,zero,zero,rof1,rof1);
delay(lambda*tau1); /* (lambda)t1/2 */
}
delay(tau1); /* t1/2 */
delay(taunco - tauhn - pw180onco);
decpwrf(rf180onco);
decrgpulse(pw180onco,zero,0.0,0.0); /* 180 for 13C' */
delay((1-kappa)*tau1); /* (1-k)t1/2 */
dec2rgpulse(2.0*pwN,zero,0.0,0.0); /* 180 for 15N */
delay((taunco - tauhn - gt1 - gstab) - kappa*tau1); /* 1/4J(NCO) - 1/4J(NH) - kt1/2 */
zgradpulse(gzlvl1,gt1);
delay(gstab);
/* start TROSY transfer from N to HN */
sim3pulse(pw,0.0,0.0,t2,zero,zero,rof1,rof1);
zgradpulse(gzlvl5,gt5);
delay(gstab);
delay(tauhn - gt5 - gstab - POWER_DELAY);
decpwrf(rf180onco);
sim3pulse(2.0*pw,pw180onco,2*pwN,zero,zero,zero,rof1,rof1);
delay(tauhn - gt5 - gstab - POWER_DELAY);
zgradpulse(gzlvl5,gt5);
delay(gstab);
decpwrf(rf90onco);
sim3pulse(pw,pw90onco,pwN,one,t6,zero,rof1,rof1);
/* shaped pulse WATERGATE */
obspower(tpwrs); obspwrf(tpwrsf_u);
shaped_pulse("H2Osinc_u",pwHs,t2,rof1,0.0);
obspower(tpwr); obspwrf(4095.0);
/* shaped pulse */
zgradpulse(gzlvl5*0.8,gt5);
delay(gstab);
delay(tauhn - gt5 - gstab - 2.0*POWER_DELAY - pwHs);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,rof1,rof1);
delay(tauhn - gt5 - gstab - 2.0*POWER_DELAY);
decpower(dpwr);
zgradpulse(gzlvl5*0.8,gt5);
delay(gstab);
dec2rgpulse(pwN,t3,0.0,0.0); /* 90 for 15N */
dec2power(dpwr2);
delay((gt1/10.0) -pwN + gstab - POWER_DELAY);
rgpulse(2.0*pw, zero, rof1, rof1);
zgradpulse(gzlvl2*icosel,gt1/10.0);
delay(gstab);
status(C);
setreceiver(t7);
}
pulsesequence()
{
/* DECLARE AND LOAD VARIABLES */
char f1180[MAXSTR],
bottom[MAXSTR],
right[MAXSTR],
C13refoc[MAXSTR];
int t1_counter;
double tau1, /* t1 delay */
taua=getval("taua"), /* < 1 / 4J(NH) 2.25 ms */
taub=getval("taub"), /* 1 / 4J(NH) 2.75 ms */
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
compH = getval("compH"), /* adjustment for H1 amplifier compression */
compN = getval("compN"), /* adjustment for N15 amplifier compression */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
pwHs = getval("pwHs"), /* H1 90 degree pulse length at tpwrs */
tpwrs, /* power for the pwHs ("H2Osinc") pulse */
finepwrf = getval("finepwrf"), /* fine power adjustment */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
sw1 = getval("sw1"),
gt3 = getval("gt3"),
gt4 = getval("gt4"),
gt5 = getval("gt5"),
gzlvlr = getval("gzlvlr"),
gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5");
getstr("f1180",f1180);
getstr("bottom",bottom);
getstr("right",right);
getstr("C13refoc",C13refoc);
/* INITIALIZE VARIABLES */
compN=compN;
compC=compC;
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /*needs 1.69 times more*/
tpwrs = (int) (tpwrs); /*power than a square pulse */
tpwrs = tpwrs+6; /* increase attenuator setting so that
fine power control can be varied about the nominal 2048 value */
/* check validity of parameter range */
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y'))
{
printf("incorrect Dec1 decoupler flags! ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y'))
{
printf("incorrect Dec2 decoupler flags! ");
psg_abort(1);
}
if( dpwr > 50 )
{
printf("don't fry the probe, dpwr too large! ");
psg_abort(1);
}
if( dpwr2 > 50 )
{
printf("don't fry the probe, dpwr2 too large! ");
psg_abort(1);
}
if( pwNlvl > 63 )
{
printf("don't fry the probe, pwNlvl too large !");
psg_abort(1);
}
if( pwClvl > 63 )
{
printf("don't fry the probe, pwClvl too large !");
psg_abort(1);
}
if( gzlvlr > 500 )
{
printf("don't fry the probe, gzlvl0 too large !");
psg_abort(1);
}
/* LOAD VARIABLES */
settable(t1, 8, phi1);
if (bottom[A]=='y')
{
settable(t2, 1, ph_y);
settable(t5, 2, phi5);
settable(t7, 2, phi6);
}
else
{
settable(t2, 1, phy);
settable(t5, 2, phi6);
settable(t7, 2, phi5);
}
if (right[A]=='y')
settable(t3, 1, ph_y);
else
settable(t3, 1, phy);
settable(t4, 8, phi4);
settable(t6, 8, rec);
/* Set up f1180 */
tau1 = d2;
if(f1180[A] == 'y' && C13refoc[A] == 'n')
tau1 += ( 1.0 / (2.0*sw1) - pw);
if(f1180[A] == 'y' && C13refoc[A] == 'y')
tau1 += ( 1.0 / (2.0*sw1) - 2.0*pwC - 4.0e-6 - pw);
if(f1180[A] == 'n' && C13refoc[A] == 'n')
tau1 = tau1 - pw;
if(f1180[A] == 'n' && C13refoc[A] == 'y')
tau1 = ( tau1 - 2.0*pwC - 4.0e-6 - pw);
if(tau1 < 0.2e-6) tau1 = 0.2e-6;
/* set up for complex FID */
if (phase1 == 2)
{
tsadd(t2,2,4);
tsadd(t3,2,4);
tsadd(t6,2,4);
}
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1 ) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if( t1_counter %2 ) {
tsadd(t1,2,4);
tsadd(t4,2,4);
tsadd(t6,2,4);
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obsoffset(tof);
decoffset(dof+(50*dfrq)); /* 160 ppm for DEC */
dec2offset(dof2-(45*dfrq2)); /* Middle of imino-N15 = 155 ppm */
obspower(tpwr);
obspwrf(4095.0);
decpower(pwClvl);
dec2power(pwNlvl);
txphase(two);
decphase(zero);
dec2phase(zero);
delay(d1);
rcvroff();
delay(20.0e-6);
rgpulse(pw,two,0.0,0.0);
txphase(zero);
zgradpulse(gzlvl5, gt5);
delay(taua - gt5 - 2.0*GRADIENT_DELAY); /* delay < 1/4J(XH) */
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
txphase(one);
zgradpulse(gzlvl5, gt5);
delay(taua - gt5 - 2.0*GRADIENT_DELAY);
if (phase1 == 1)
sim3pulse(pw,0.0,pwN,one,zero,t1,0.0,0.0);
if (phase1 == 2)
sim3pulse(pw,0.0,pwN,one,zero,t4,0.0,0.0);
txphase(zero);
decphase(zero);
if (C13refoc[A] == 'y')
{
if (tau1>0.001)
{
zgradpulse(gzlvlr,(0.8*tau1/2.0 - 2.0*GRADIENT_DELAY ));
delay(0.2*tau1/2.0 );
}
else
delay(tau1/2.0);
decrgpulse(pwC,zero,0.0,0.0);
decrgpulse(2.0*pwC,one,2.0e-6,0.0);
decrgpulse(pwC,zero,2.0e-6,0.0);
if (tau1>0.001)
{
zgradpulse(-gzlvlr,(0.8*tau1/2.0 - 2.0*GRADIENT_DELAY ));
delay(0.2*tau1/2.0 );
}
else
if ((tau1/2.0)>0.0) delay(tau1/2.0);
}
else
{
if (tau1>0.001)
{
zgradpulse(gzlvlr,(0.8*tau1 - 2.0*GRADIENT_DELAY));
delay(0.2*tau1);
zgradpulse(-gzlvlr,(0.8*tau1 - 2.0*GRADIENT_DELAY ));
delay(0.2*tau1);
}
else
delay(tau1);
}
rgpulse(pw,t2,0.0,0.0);
txphase(zero);
dec2phase(zero);
zgradpulse(gzlvl3,gt3);
delay(taub - gt3 - 2.0*GRADIENT_DELAY);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
dec2phase(t3);
zgradpulse(gzlvl3,gt3);
delay(taub - gt3 - 2.0*GRADIENT_DELAY);
sim3pulse(pw,0.0,pwN,zero,zero,t3,0.0,0.0);
txphase(t5);
dec2phase(zero);
obspower(tpwrs);
obspwrf(finepwrf);
zgradpulse(gzlvl4,gt4);
delay(taub - gt4 - 4.0*POWER_DELAY - rof1 - 2.0*GRADIENT_DELAY - pwHs);
shaped_pulse("H2Osinc",pwHs,t5,0.0,0.0);
txphase(t7);
obspower(tpwr);
obspwrf(4095.0);
sim3pulse(2.0*pw,0.0e-6,2.0*pwN,t7,zero,zero,rof1,rof1);
txphase(t5);
obspwrf(finepwrf);
obspower(tpwrs);
shaped_pulse("H2Osinc",pwHs,t5,0.0,0.0);
txphase(zero);
obspower(tpwr);
obspwrf(4095.0);
zgradpulse(gzlvl4,gt4);
delay(taub - gt4 - 6.0*POWER_DELAY - rof1 - 2.0*GRADIENT_DELAY
- 0.5*(pwN-pw) - pwHs);
dec2rgpulse(pwN,zero,0.0,0.0);
decpower(dpwr);
dec2power(dpwr2);
/* acquire data */
status(C);
setreceiver(t6);
}
pulsesequence()
{
/* DECLARE VARIABLES */
char satmode[MAXSTR],
fscuba[MAXSTR],
fc180[MAXSTR], /* Flag for checking sequence */
cbdecseq[MAXSTR];
int icosel,
ni = getval("ni"),
t1_counter; /* used for states tppi in t1 */
double tau1, /* t1 delay */
tau2, /* t2 delay */
tau3, /* t2 delay */
taua, /* ~ 1/4JNH = 2.25 ms */
taub, /* ~ 1/4JNH = 2.25 ms */
tauc, /* ~ 1/4JCaC' = 4 ms */
taud, /* ~ 1/4JCaC' = 4.5 ms if bigTCo can be set to be
less than 4.5ms and then taud can be smaller*/
zeta, /* time for C'-N to refocuss set to 0.5*24.0 ms */
bigTCa, /* Ca T period */
bigTCo, /* Co T period */
bigTN, /* nitrogen T period */
BigT1, /* delay to compensate for gradient gt5 */
satpwr, /* low level 1H trans.power for presat */
sw1, /* sweep width in f1 */
sw2, /* sweep width in f2 */
sphase, /* small angle phase shift */
cbpwr, /* power level for selective CB decoupling */
cbdmf, /* pulse width for selective CB decoupling */
cbres, /* decoupling resolution of CB decoupling */
pwS1, /* length of square 90 on Ca */
phshift, /* phase shift induced on Ca by 180 on CO in middle of t1 */
pwS2, /* length of 180 on CO */
pwS3, /* length of 180 on Ca */
pwS4, /* length of 90 on CO */
pwS = getval("pwS"), /* used to change 180 on CO in t1 for 1D calibrations */
pwZ, /* the largest of pwS2 and 2.0*pwN */
pwZ1, /* the largest of pwS2 and 2.0*pwN for 1D experiments */
gt1,
gt2,
gt3,
gt4,
gt5,
gt6,
gt7,
gt8,
gt9,
gt10,
gt11,
gt12,
gstab,
gzlvl1,
gzlvl2,
gzlvl3,
gzlvl4,
gzlvl5,
gzlvl6,
gzlvl7,
gzlvl8,
gzlvl9,
gzlvl10,
gzlvl11,
gzlvl12,
compH = getval("compH"), /* adjustment for amplifier compression */
pwHs = getval ("pwHs"), /* H1 90 degree pulse at tpwrs */
tpwrs, /* power for pwHs ("H2osinc") pulse */
waltzB1 = getval("waltzB1"),
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
swCa = getval("swCa"),
swCO = getval("swCO"),
swN = getval("swN"),
swTilt, /* This is the sweep width of the tilt vector */
cos_N, cos_CO, cos_Ca,
angle_N, angle_CO, angle_Ca;
angle_N=0.0; /*initialize variable*/
/* LOAD VARIABLES */
getstr("satmode",satmode);
getstr("fc180",fc180);
getstr("fscuba",fscuba);
taua = getval("taua");
taub = getval("taub");
tauc = getval("tauc");
taud = getval("taud");
zeta = getval("zeta");
bigTCa = getval("bigTCa");
bigTCo = getval("bigTCo");
bigTN = getval("bigTN");
BigT1 = getval("BigT1");
tpwr = getval("tpwr");
satpwr = getval("satpwr");
dpwr = getval("dpwr");
sw1 = getval("sw1");
sw2 = getval("sw2");
sphase = getval("sphase");
gt1 = getval("gt1");
gt2 = getval("gt2");
gt3 = getval("gt3");
gt4 = getval("gt4");
gt5 = getval("gt5");
gt6 = getval("gt6");
gt7 = getval("gt7");
gt8 = getval("gt8");
gt9 = getval("gt9");
gt10 = getval("gt10");
gt11 = getval("gt11");
gt12 = getval("gt12");
gstab = getval("gstab");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl3 = getval("gzlvl3");
gzlvl4 = getval("gzlvl4");
gzlvl5 = getval("gzlvl5");
gzlvl6 = getval("gzlvl6");
gzlvl7 = getval("gzlvl7");
gzlvl8 = getval("gzlvl8");
gzlvl9 = getval("gzlvl9");
gzlvl10 = getval("gzlvl10");
gzlvl11 = getval("gzlvl11");
gzlvl12 = getval("gzlvl12");
/* Load variable */
cbpwr = getval("cbpwr");
cbdmf = getval("cbdmf");
cbres = getval("cbres");
tau1 = 0;
tau2 = 0;
tau3 = 0;
cos_N = 0;
cos_CO = 0;
cos_Ca = 0;
getstr("cbdecseq", cbdecseq);
/* LOAD PHASE TABLE */
settable(t1,1,phi1);
settable(t2,1,phi2);
settable(t3,4,phi3);
settable(t4,1,phi4);
settable(t5,1,phi5);
settable(t7,4,phi7);
settable(t8,4,phi8);
settable(t6,4,rec);
kappa = 5.4e-3;
/* get calculated pulse lengths of shaped C13 pulses */
pwS1 = c13pulsepw("ca", "co", "square", 90.0);
pwS2 = c13pulsepw("co", "ca", "sinc", 180.0);
pwS3 = c13pulsepw("ca","co","square",180.0);
pwS4 = c13pulsepw("co", "ca", "sinc", 90.0);
/* the 180 pulse on CO at the middle of t1 */
if (pwS2 > 2.0*pwN) pwZ = pwS2; else pwZ = 2.0*pwN;
if ((pwS==0.0) && (pwS2>2.0*pwN)) pwZ1=pwS2-2.0*pwN; else pwZ1=0.0;
if ( ni > 1 ) pwS = 180.0;
if ( pwS > 0 ) phshift = 140.0;
else phshift = 0.0;
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /*needs 1.69 times more*/
tpwrs = (int) (tpwrs); /*power than a square pulse */
widthHd = 2.681*waltzB1/sfrq; /* bandwidth of H1 WALTZ16 decoupling */
pwHd = h1dec90pw("WALTZ16", widthHd, 0.0); /* H1 90 length for WALTZ16 */
/* CHECK VALIDITY OF PARAMETER RANGES */
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y' ))
{
printf("incorrect dec1 decoupler flags! ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' ))
{
printf("incorrect dec2 decoupler flags! Should be 'nny' ");
psg_abort(1);
}
if( satpwr > 6 )
{
printf("TSATPWR too large !!! ");
psg_abort(1);
}
if( dpwr > 46 )
{
printf("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if( dpwr2 > 46 )
{
printf("don't fry the probe, DPWR2 too large! ");
psg_abort(1);
}
if( pwClvl > 62 )
{
printf("don't fry the probe, pwClvl too large! ");
psg_abort(1);
}
if( pw > 200.0e-6 )
{
printf("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwN > 200.0e-6 )
{
printf("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
if( pwC > 200.0e-6 )
{
printf("dont fry the probe, pwC too high ! ");
psg_abort(1);
}
if( pwS3 > 200.0e-6 )
{
printf("dont fry the probe, pwS3 too high ! ");
psg_abort(1);
}
if( gt3 > 2.5e-3 )
{
printf("gt3 is too long\n");
psg_abort(1);
}
if( gt1 > 10.0e-3 || gt2 > 10.0e-3 || gt4 > 10.0e-3 || gt5 > 10.0e-3
|| gt6 > 10.0e-3 || gt7 > 10.0e-3 || gt8 > 10.0e-3
|| gt9 > 10.0e-3 || gt10 > 10.0e-3 || gt11 > 50.0e-6)
{
printf("gt values are too long. Must be < 10.0e-3 or gt11=50us\n");
psg_abort(1);
}
/* PHASES AND INCREMENTED TIMES */
/* Set up angles and phases */
angle_CO=getval("angle_CO"); cos_CO=cos(PI*angle_CO/180.0);
angle_Ca=getval("angle_Ca"); cos_Ca=cos(PI*angle_Ca/180.0);
if ( (angle_CO < 0) || (angle_CO > 90) )
{ printf ("angle_CO must be between 0 and 90 degree.\n"); psg_abort(1); }
if ( (angle_Ca < 0) || (angle_Ca > 90) )
{ printf ("angle_Ca must be between 0 and 90 degree.\n"); psg_abort(1); }
if ( 1.0 < (cos_CO*cos_CO + cos_Ca*cos_Ca) )
{
printf ("Impossible angles.\n"); psg_abort(1);
}
else
{
cos_N=sqrt(1.0- (cos_CO*cos_CO + cos_Ca*cos_Ca));
angle_N = 180.0*acos(cos_N)/PI;
}
swTilt=swCO*cos_CO + swCa*cos_Ca + swN*cos_N;
if (ix ==1)
{
printf("\n\nn\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n");
printf ("Maximum Sweep Width: \t\t %f Hz\n", swTilt);
printf ("Angle_CO:\t%6.2f\n", angle_CO);
printf ("Angle_Ca:\t%6.2f\n", angle_Ca);
printf ("Angle_N :\t%6.2f\n", angle_N );
}
/* Set up hyper complex */
/* sw1 is used as symbolic index */
if ( sw1 < 1000 ) { printf ("Please set sw1 to some value larger than 1000.\n"); psg_abort(1); }
if (ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if (t1_counter % 2) { tsadd(t2,2,4); tsadd(t6,2,4); }
if (phase1 == 1) { ;} /* CC */
else if (phase1 == 2) { tsadd(t5,1,4);} /* SC */
else if (phase1 == 3) { tsadd(t1,1,4); } /* CS */
else if (phase1 == 4) { tsadd(t5,1,4); tsadd(t1,1,4); } /* SS */
else { printf ("phase1 can only be 1,2,3,4. \n"); psg_abort(1); }
if (phase2 == 2) { tsadd(t4,2,4); icosel = 1; } /* N */
else icosel = -1;
tau1 = 1.0*t1_counter*cos_Ca/swTilt;
tau2 = 1.0*t1_counter*cos_CO/swTilt;
tau3 = 1.0*t1_counter*cos_N/swTilt;
tau1 = tau1/2.0; tau2 = tau2/2.0; tau3 = tau3/2.0;
/* CHECK VALIDITY OF PARAMETER RANGES */
if (bigTN - 0.5*ni*(cos_N/swTilt) + pwS2 < 0.2e-6)
{ printf(" ni is too big. Make ni equal to %d or less.\n",
((int)((bigTN + pwS2)*2.0*swTilt/cos_N))); psg_abort(1);}
if (bigTCo - 0.5*ni*(cos_CO/swTilt) - 4.0e-6 - POWER_DELAY < 0.2e-6)
{
printf(" ni is too big for CO. Make ni equal to %d or less.\n",
(int) ((bigTCo - 4.0e-6 - POWER_DELAY) / (0.5*cos_CO/swTilt)) );
psg_abort(1);
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obsoffset(tof);
set_c13offset("ca"); /* set Dec1 carrier at Co */
obspower(satpwr); /* Set transmitter power for 1H presaturation */
obspwrf(4095.0);
decpower(pwClvl); /* Set Dec1 power for hard 13C pulses */
decpwrf(4095.0);
dec2power(pwNlvl); /* Set Dec2 power for hard 15N pulses */
dec2pwrf(4095.0);
sim3_c13pulse("", "ca", "co", "sinc", "", 0.0, 180.0, 0.0, /* to produce shape */
zero, zero, zero, 2.0e-6, 2.0e-4);
set_c13offset("co"); /* set Dec1 carrier at Co */
/* Presaturation Period */
if (satmode[0] == 'y')
{
delay(2.0e-5);
rgpulse(d1,zero,2.0e-6,2.0e-6); /* presaturation */
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
delay(2.0e-5);
if(fscuba[0] == 'y')
{
delay(2.2e-2);
rgpulse(pw,zero,2.0e-6,0.0);
rgpulse(2*pw,one,2.0e-6,0.0);
rgpulse(pw,zero,2.0e-6,0.0);
delay(2.2e-2);
}
}
else
{
delay(d1);
}
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
txphase(three);
dec2phase(zero);
delay(1.0e-5);
/* Begin Pulses */
status(B);
rcvroff();
shiftedpulse("sinc", pwHs, 90.0, 0.0, three, 2.0e-6, 2.0e-6);
txphase(zero);
/* xxxxxxxxxxxxxxxxxxxxxx 1HN to 15N TRANSFER xxxxxxxxxxxxxxxxxx */
rgpulse(pw,zero,0.0,0.0); /* 90 deg 1H pulse */
delay(0.2e-6);
zgradpulse(gzlvl1, gt1);
delay(2.0e-6);
delay(taua - gt1 - 2.2e-6); /* taua <= 1/4JNH */
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
txphase(one); dec2phase(zero); decphase(zero);
delay(taua - gt1 - gstab -0.2e-6 - 2.0e-6);
delay(0.2e-6);
zgradpulse(gzlvl1, gt1);
delay(gstab);
/* xxxxxxxxxxxxxxxxxxxxxx 15N to 13CO TRANSFER xxxxxxxxxxxxxxxxxx */
rgpulse(pw,one,2.0e-6,0.0);
delay(0.2e-6);
zgradpulse(gzlvl2, gt2);
delay(gstab);
dec2rgpulse(pwN,zero,0.0,0.0);
delay(kappa - POWER_DELAY - PWRF_DELAY - pwHd - 4.0e-6 - PRG_START_DELAY);
/* delays for h1waltzon subtracted */
h1waltzon("WALTZ16", widthHd, 0.0);
decphase(zero);
dec2phase(zero);
delay(zeta - kappa - WFG3_START_DELAY);
dec2rgpulse(2*pwN,zero,0.0,0.0);
c13pulse("co", "ca", "sinc", 180.0, zero, 0.0, 0.0);
dec2phase(zero);
delay(zeta - 2.0e-6);
dec2rgpulse(pwN,zero,2.0e-6,0.0);
h1waltzoff("WALTZ16", widthHd, 0.0);
dec2phase(zero); decphase(zero);
delay(0.2e-6);
/* CHECK Negative gradient */
zgradpulse(-gzlvl3, gt3);
delay(gstab);
/* xxxxxxxxxxxxxxxxxxxxx 13CO to 13CA TRANSFER xxxxxxxxxxxxxxxxxxxxxxx */
c13pulse("co", "ca", "sinc", 90.0, zero, 2.0e-6, 0.0);
delay(2.0e-7);
zgradpulse(gzlvl10, gt10);
delay(tauc - gt10 - 0.2e-6 - (0.5*10.933*pwC));
decrgpulse(pwC*158.0/90.0, zero, 0.0, 0.0);
decrgpulse(pwC*171.2/90.0, two, 0.0, 0.0);
decrgpulse(pwC*342.8/90.0, zero, 0.0, 0.0); /* Shaka 6 composite */
decrgpulse(pwC*145.5/90.0, two, 0.0, 0.0);
decrgpulse(pwC*81.2/90.0, zero, 0.0, 0.0);
decrgpulse(pwC*85.3/90.0, two, 0.0, 0.0);
delay(2.0e-7);
zgradpulse(gzlvl10, gt10);
delay(tauc - 4.0e-6 - gt10 - 0.2e-6 - (0.5*10.933*pwC));
c13pulse("co", "ca", "sinc", 90.0, one, 4.0e-6, 0.0);
set_c13offset("ca"); /* change Dec1 carrier to Ca (55 ppm) */
delay(0.2e-6);
zgradpulse(gzlvl9, gt9);
decphase(t5);
delay(gstab);
/* xxxxxxxxxxxxxxxxxx 13CA EVOLUTION xxxxxxxxxxxxxxxxxxxxxx */
h1waltzon("WALTZ16", widthHd, 0.0);
c13pulse("ca", "co", "square", 90.0, t5, 2.0e-6, 0.0); /* pwS1 */
if (fc180[A]=='n')
{
if ((ni>1.0) && (tau1>0.0))
{
if (tau1 - 2.0*pwS1/PI - WFG3_START_DELAY - PRG_START_DELAY - 2*POWER_DELAY -
PRG_STOP_DELAY - 2.0*PWRF_DELAY - 0.5*pwZ > 0.0)
{
decpower(cbpwr);
decphase(zero);
decprgon(cbdecseq,1/cbdmf,cbres);
decon();
delay(tau1 - 2.0*pwS1/PI - WFG3_START_DELAY - PRG_START_DELAY - 2*POWER_DELAY -
PRG_STOP_DELAY - 2.0*PWRF_DELAY - 0.5*pwZ);
decoff();
decprgoff();
decpower(pwClvl);
decphase(zero); dec2phase(zero);
sim3_c13pulse("", "co", "ca", "sinc", "", 0.0, 180.0, 2.0*pwN,
zero, zero, zero, 2.0e-6, 2.0e-6);
decpower(cbpwr);
decphase(zero);
decprgon(cbdecseq,1/cbdmf,cbres);
decon();
delay(tau1 - 2.0*pwS1/PI - PRG_START_DELAY - 2*POWER_DELAY -
PRG_STOP_DELAY - WFG_START_DELAY - 2.0*PWRF_DELAY - 0.5*pwZ);
decoff();
decprgoff();
decpower(pwClvl);
initval(140.0, v9);
decstepsize(1.0);
dcplrphase(v9);
}
else
{
tsadd(t6,2,4);
delay(2.0*tau1);
delay(10.0e-6);
sim3_c13pulse("", "ca", "co", "sinc", "", 0.0, 180.0, 2.0*pwN,
zero, zero, zero, 2.0e-6, 2.0e-6);
delay(10.0e-6);
}
}
else
{
tsadd(t6,2,4);
delay(10.0e-6);
sim3_c13pulse("", "co", "ca", "sinc", "", 0.0, 180.0, 2.0*pwN,
zero, zero, zero, 2.0e-6, 2.0e-6);
delay(10.0e-6);
}
}
else
{
/* for checking sequence */
c13pulse("ca", "co", "square", 180.0, zero, 2.0e-6, 0.0);
}
decphase(t7);
c13pulse("ca", "co", "square", 90.0, t7, 4.0e-6, 0.0); /* pwS1 */
dcplrphase(zero);
h1waltzoff("WALTZ16", widthHd, 0.0);
set_c13offset("co"); /* set carrier back to Co */
delay(0.2e-6);
zgradpulse(gzlvl12, gt12);
delay(gstab);
/* xxxxxxxxxxxxxxx 13CA to 13CO TRANSFER and CT 13CO EVOLUTION xxxxxxxxxxxxxxxxx */
c13pulse("co", "ca", "sinc", 90.0, t1, 2.0e-6, 0.0);
delay(tau2);
dec2rgpulse(pwN,one,0.0,0.0);
dec2rgpulse(2*pwN,zero,0.0,0.0);
dec2rgpulse(pwN,one,0.0,0.0);
delay(taud - 4.0*pwN - POWER_DELAY
- 0.5*(WFG_START_DELAY + pwS3 + WFG_STOP_DELAY));
c13pulse("ca", "co", "square", 180.0, zero, 2.0e-6, 0.0);
decphase(t8);
initval(1.0,v4);
decstepsize(sphase);
dcplrphase(v4);
delay(bigTCo - taud
- 0.5*(WFG_START_DELAY + pwS3 + WFG_STOP_DELAY) );
c13pulse("co", "ca", "sinc", 180.0, t8, 2.0e-6, 0.0);
dcplrphase(zero); decphase(one);
delay(bigTCo - tau2 - POWER_DELAY - 4.0e-6);
c13pulse("co", "ca", "sinc", 90.0, one, 4.0e-6, 0.0);
delay(0.2e-6);
zgradpulse(gzlvl4, gt4);
delay(gstab);
h1waltzon("WALTZ16", widthHd, 0.0);
/* t3 period */
dec2rgpulse(pwN,t2,2.0e-6,0.0);
dec2phase(t3);
delay(bigTN - tau3 + pwS2);
dec2rgpulse(2*pwN,t3,0.0,0.0);
c13pulse("co", "ca", "sinc", 180.0, zero, 0.0, 0.0);
dec2phase(t4);
if (tau3 > (kappa + PRG_STOP_DELAY + pwHd + 2.0e-6))
{
delay(bigTN - pwS3 - WFG_START_DELAY - 2.0*POWER_DELAY
- 2.0*PWRF_DELAY - 2.0e-6);
c13pulse("ca", "co", "square", 180.0, zero, 2.0e-6, 0.0);
delay(tau3 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6 - POWER_DELAY
- PWRF_DELAY);
h1waltzoff("WALTZ16", widthHd, 0.0);
txphase(zero);
delay(kappa - gt5 - 2.0*GRADIENT_DELAY - 1.0e-4);
zgradpulse(gzlvl5, gt5); /* 2.0*GRADIENT_DELAY */
delay(1.0e-4);
}
else if (tau3 > (kappa - pwS3 - WFG_START_DELAY - 2.0*POWER_DELAY
- 2.0*PWRF_DELAY - 2.0e-6))
{
delay(bigTN + tau3 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6
- POWER_DELAY - PWRF_DELAY);
h1waltzoff("WALTZ16", widthHd, 0.0);
txphase(zero);
c13pulse("ca", "co", "square", 180.0, zero, 2.0e-6, 0.0);
delay(kappa - pwS3 - WFG_START_DELAY - 2.0*POWER_DELAY
- 2.0*PWRF_DELAY - 2.0e-6 - gt5 - 2.0*GRADIENT_DELAY - 1.0e-4);
zgradpulse(gzlvl5, gt5); /* 2.0*GRADIENT_DELAY */
delay(1.0e-4);
}
else if (tau3 > gt5 + 2.0*GRADIENT_DELAY + 1.0e-4)
{
delay(bigTN + tau3 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6
- POWER_DELAY - PWRF_DELAY);
h1waltzoff("WALTZ16", widthHd, 0.0);
txphase(zero);
delay(kappa - tau3 - pwS3 - WFG_START_DELAY - 2.0*POWER_DELAY
- 2.0*PWRF_DELAY - 2.0e-6);
c13pulse("ca", "co", "square", 180.0, zero, 2.0e-6, 0.0);
delay(tau3 - gt5 - 2.0*GRADIENT_DELAY - 1.0e-4);
zgradpulse(gzlvl5, gt5); /* 2.0*GRADIENT_DELAY */
delay(1.0e-4);
}
else
{
delay(bigTN + tau3 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6
- POWER_DELAY - PWRF_DELAY);
h1waltzoff("WALTZ16", widthHd, 0.0);
txphase(zero);
delay(kappa - tau3 - pwS3 - WFG_START_DELAY - 2.0*POWER_DELAY
- 2.0*PWRF_DELAY - 2.0e-6 - gt5 - 2.0*GRADIENT_DELAY - 1.0e-4);
zgradpulse(gzlvl5, gt5); /* 2.0*GRADIENT_DELAY */
delay(1.0e-4);
c13pulse("ca", "co", "square", 180.0, zero, 2.0e-6, 0.0);
delay(tau3);
}
sim3pulse(pw,0.0,pwN,zero,zero,t4,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl6, gt6);
delay(2.0e-6);
dec2phase(zero);
delay(taub - gt6 - 2.2e-6);
sim3pulse(2.0*pw,0.0,2.0*pwN,zero,zero,zero,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl6, gt6);
delay(200.0e-6);
txphase(one);
dec2phase(one);
delay(taub - gt6 - 200.2e-6);
sim3pulse(pw,0.0,pwN,one,zero,one,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7, gt7);
delay(2.0e-6);
txphase(zero);
dec2phase(zero);
delay(taub - gt7 - 2.2e-6);
sim3pulse(2.0*pw,0.0,2.0*pwN,zero,zero,zero,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7, gt7);
delay(200.0e-6);
delay(taub - gt7 - 200.2e-6);
rgpulse(pw, zero, 0.0, 0.0);
delay(gt8 + 1.0e-4 + 1.0e-4 - 0.3*pw + 2.0*GRADIENT_DELAY
+ POWER_DELAY);
rgpulse(2*pw,zero,0.0,0.0);
dec2power(dpwr2);
decpower(dpwr);
zgradpulse(icosel*gzlvl8, gt8);
delay(1.0e-4);
/* rcvron(); */ /* Turn on receiver to warm up before acq */
/* BEGIN ACQUISITION */
statusdelay(C, 1.0e-4);
setreceiver(t6);
}
pulsesequence()
{
double j1min = getval("j1min"),
j1max = getval("j1max"),
gzlvlE = getval("gzlvlE"),
gtE = getval("gtE"),
mult = getval("mult"),
pwx180 = getval("pwx180"),
pwxlvl180 = getval("pwxlvl180"),
pw180 = getval("pw180"),
pwx180r = getval("pwx180r"),
pwxlvl180r = getval("pwxlvl180r"),
tpwr180 = getval("tpwr180"),
EDratio = getval("EDratio"),
hsglvl = getval("hsglvl"),
hsgt = getval("hsgt"),
gstab = getval("gstab"),
dmfct = getval("dmfct"),
dpwrct = getval("dpwrct"),
tau,
tau1,
tau3,
taug,
bigT = getval("BigT");
char pwx180ad[MAXSTR],
pw180ad[MAXSTR],
dmct[MAXSTR],
bipflg[MAXSTR],
pwx180ref[MAXSTR],
dmmct[MAXSTR];
int icosel,
igcorr,
prgcycle = (int)(getval("prgcycle")+0.5),
phase1 = (int)(getval("phase")+0.5);
//synchronize gradients to srate for probetype='nano'
// Preserve gradient "area"
gtE = syncGradTime("gtE","gzlvlE",1.0);
gzlvlE = syncGradLvl("gtE","gzlvlE",1.0);
getstr("pwx180ad",pwx180ad);
getstr("pw180ad",pw180ad);
getstr("dmct",dmct);
getstr("pwx180ref", pwx180ref);
getstr("dmmct", dmmct);
getstr("bipflg",bipflg);
if (bipflg[0] == 'y')
{
tpwr180=getval("tnbippwr");
pw180=getval("tnbippw");
getstr("tnbipshp",pw180ad);
}
if (bipflg[1] == 'y')
{
pwxlvl180=getval("dnbippwr");
pwx180=getval("dnbippw");
getstr("dnbipshp",pwx180ad);
}
tau1 = 1/(2*(j1min + 0.07*(j1max - j1min)));
tau3 = 1/(2*(j1max - 0.07*(j1max - j1min)));
tau = 1 / (j1min+j1max);
taug = tau + getval("tauC");
icosel = 1;
igcorr = 2;
if (mult > 0.5)
igcorr = 1;
assign(ct,v17);
assign(zero,v18);
assign(zero,v19);
if (getflag("prgflg") && (satmode[0] == 'y') && (prgcycle > 1.5))
{
hlv(ct,v17);
mod2(ct,v18); dbl(v18,v18);
if (prgcycle > 2.5)
{
hlv(v17,v17);
hlv(ct,v19); mod2(v19,v19); dbl(v19,v19);
}
}
settable(t1, 4, ph1);
settable(t2, 8, ph2);
settable(t3, 16, ph3);
settable(t6, 2, ph6);
getelem(t1, v17, v1);
getelem(t3, v17, v3);
getelem(t2, v17, v2);
getelem(t6, v17, oph);
assign(zero, v4);
assign(zero,v6);
add(oph,v18,oph);
add(oph,v19,oph);
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v14);
/*
mod2(id2, v14);
dbl(v14,v14);
*/
add(v2, v14, v2);
add(v4, v14, v4);
add(oph, v14, oph);
if ((phase1 == 2) || (phase1 == 5))
icosel = -1;
status(A);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
if (satmode[0] == 'y')
{
if ((d1-satdly) > 0.02)
delay(d1-satdly);
else
delay(0.02);
if (getflag("slpsat"))
{
shaped_satpulse("relaxD",satdly,zero);
if (getflag("prgflg"))
shaped_purge(v6,zero,v18,v19);
}
else
{
satpulse(satdly,zero,rof1,rof1);
if (getflag("prgflg"))
purge(v6,zero,v18,v19);
}
}
else
delay(d1);
if (getflag("wet"))
wet4(zero,one);
obspower(tpwr);
decpower(pwxlvl);
if (getflag("nullflg"))
{
decpower(pwxlvl180);
rgpulse(0.5 * pw, zero, rof1, rof1);
obspower(tpwr180);
delay(tau/2.0);
simshaped_pulse(pw180ad,pwx180ad,pw180,pwx180,zero,zero,rof1,rof1);
delay(tau/2.0+POWER_DELAY+rof1);
delay(tau/2.0+POWER_DELAY+rof1);
simshaped_pulse(pw180ad,pwx180ad,pw180,pwx180,two,two,rof1,rof1);
obspower(tpwr);
delay(tau/2.0);
rgpulse(0.5 * pw, zero, rof1, rof1);
decpower(pwxlvl);
zgradpulse(hsglvl, 1.4*hsgt);
delay(1e-3);
}
status(B);
rgpulse(pw, zero, rof1, rof1);
if (getflag("dmct"))
{
decpower(dpwrct);
decprgon("garp1",1/dmfct,1.0);
/*
setstatus(DECch, FALSE, 'g', FALSE, dmfct);
*/
decunblank();
decon();
}
obspower(tpwr180);
delay(bigT/2 - d2/2 - tau - taug - pwx180r);
delay(pw180+WFG_START_DELAY+WFG_STOP_DELAY);
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
delay(bigT/2 - d2/2 - tau - taug - pwx180r);
delay(4*pw/PI+2*rof1+POWER_DELAY+4*pwx/PI+2*POWER_DELAY);
if (getflag("dmct"))
{
decoff();
decblank();
decprgoff();
/*
setstatus(DECch, FALSE, 'c', FALSE, dmf);
*/
decpower(pwxlvl);
}
if (mult > 0.5)
{
delay(tau-gtE-2*GRADIENT_DELAY-gstab);
zgradpulse(icosel*gzlvlE,gtE);
delay(gstab);
}
else
{
delay(tau);
}
decrgpulse(pwx,v2,rof1,rof1);
if (mult > 0.5)
{
decpower(pwxlvl180r);
delay(taug);
decshaped_pulse(pwx180ref, pwx180r, v4, rof1, rof1);
delay(d2/2);
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
delay(d2/2);
zgradpulse(icosel*gzlvlE,gtE);
delay(taug-gtE-2*GRADIENT_DELAY+(4*pwx/PI+2*rof1+2*POWER_DELAY-(WFG_START_DELAY+pw180+WFG_STOP_DELAY+2*rof1)));
decshaped_pulse(pwx180ref, pwx180r, v3, rof1, rof1);
}
else
{
decpower(pwxlvl180);
delay(taug/2+(pwx180r-pwx180)/2);
decshaped_pulse(pwx180ad, pwx180, v4, rof1, rof1);
delay(taug/2+(pwx180r-pwx180)/2.0-gtE-2*GRADIENT_DELAY-gstab);
zgradpulse(icosel*gzlvlE,gtE);
delay(gstab);
delay(d2/2);
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
delay(d2/2);
zgradpulse(icosel*gzlvlE,gtE);
delay(taug/2+(pwx180r-pwx180)/2.0+(4*pwx/PI+2*rof1+2*POWER_DELAY-WFG_START_DELAY-pw180-WFG_STOP_DELAY-2*rof1)-gtE-2*GRADIENT_DELAY);
decshaped_pulse(pwx180ad, pwx180, v3, rof1, rof1);
delay(taug/2+(pwx180r-pwx180)/2);
}
decpower(pwxlvl);
decrgpulse(pwx,v1,rof1,rof1);
obspower(tpwr);
delay(tau);
rgpulse(pw,one,rof1,rof2);
decrgpulse(pwx,zero,rof1,rof1);
obspower(tpwr180);
zgradpulse(igcorr*3*gzlvlE/(10*EDratio),gtE);
delay(tau3 - gtE - 2*GRADIENT_DELAY);
decrgpulse(pwx,zero,rof1,rof1);
zgradpulse(igcorr*7*gzlvlE/(10*EDratio),gtE);
delay(tau - gtE - 2*GRADIENT_DELAY);
decrgpulse(pwx,zero,rof1,rof1);
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
zgradpulse(igcorr*2*gzlvlE/EDratio,gtE);
delay(tau1 - gtE - 2*GRADIENT_DELAY - POWER_DELAY);
decpower(dpwr);
status(C);
delay(tau+tau3+3*pwx+6*rof1-tau1+WFG_START_DELAY+WFG_STOP_DELAY);
}
pulsesequence()
{
char f1180[MAXSTR],
f2180[MAXSTR],
mag_flg[MAXSTR],
flg_3919[MAXSTR],
ref_flg[MAXSTR];
int phase, ni2,
t1_counter,
t2_counter;
double gzcal = getval("gzcal"),
factor = 0.08, /* used for 3-9-19 water gate */
tau_3919 = getval("tau_3919"),
flipphase = getval("flipphase"),
tau1, /* t1 delay */
tau2, /* t2 delay */
taua, /* 1/4JNH ~ 2.3 ms */
taub, /* 1/4JNH ~ 2.3 ms */
bigT, /* ~ 19 ms */
pwNlvl,
pwN,
gt1,
gt2,
gt3,
gt4,
gt5,
gt6,
gt7,
gt8,
gzlvl1,
gzlvl2,
gzlvl3,
gzlvl4,
gzlvl5,
gzlvl6,
gzlvl7,
gzlvl8,
compH = getval("compH"), /* adjustment for C13 amplifier compression */
pwHs = getval("pwHs"), /* H1 90 degree pulse length at tpwrs */
tpwrsf = getval("tpwrsf"), /* fine power adjustment for flipback pulse */
tpwrs; /* power for the pwHs ("H2Osinc") pulse */
/* LOAD VARIABLES */
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("flg_3919", flg_3919);
getstr("mag_flg", mag_flg);
getstr("ref_flg", ref_flg);
taua = getval("taua");
taub = getval("taub");
bigT = getval("bigT");
tpwr = getval("tpwr");
pwNlvl = getval("pwNlvl");
pwN = getval("pwN");
dpwr = getval("dpwr");
dpwr2 = getval("dpwr2");
phase = (int)( getval("phase") + 0.5);
phase2 = (int)( getval("phase2") + 0.5);
sw1 = getval("sw1");
sw2 = getval("sw2");
ni = getval("ni");
ni2 = getval("ni2");
gt1 = getval("gt1");
gt2 = getval("gt2");
gt3 = getval("gt3");
gt4 = getval("gt4");
gt5 = getval("gt5");
gt6 = getval("gt6");
gt7 = getval("gt7");
gt8 = getval("gt8");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl3 = getval("gzlvl3");
gzlvl4 = getval("gzlvl4");
gzlvl5 = getval("gzlvl5");
gzlvl6 = getval("gzlvl6");
gzlvl7 = getval("gzlvl7");
gzlvl8 = getval("gzlvl8");
/* LOAD PHASE TABLE */
settable(t1,4,phi1);
settable(t2,2,phi2);
settable(t3,8,phi3);
settable(t4,16, phi4);
if (ref_flg[A] == 'y')
{
settable(t10,8,ref);
}
else
{
settable(t10,8,rec);
}
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /*needs 1.69 times more*/
tpwrs = (int) (tpwrs); /*power than a square pulse */
/* CHECK VALIDITY OF PARAMETER RANGES */
if (ref_flg[A] == 'y' && ni > 1)
{
printf(" Incorrect setting of ni and ref_flg.\n");
printf(" Please choose either ni=1 or ref_flg=n.\n");
psg_abort(1);
}
if (ref_flg[A] == 'y' && dps_flag)
{
printf(" Please use phase2 and ni2 for 2D reference spectrum\n");
if (ni2/sw2 > 2.0*(2.0*bigT - gt5 - 200.0e-6))
{
printf("ni2 is too big, should be < %f\n", 2.0*sw2*(2.0*bigT-gt5-200.0e-6));
psg_abort(1);
}
}
if ((ni2/sw2 > 2.0*(bigT - gt5 - 200.0e-6)) && (ref_flg[A] !='y'))
{
printf(" ni2 is too big, should be < %f\n", 2.0*sw2*(bigT-gt6-200.0e-6));
psg_abort(1);
}
if(dpwr2 > 50)
{
printf("don't fry the probe, dpwr2 is too large! ");
psg_abort(1);
}
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y'))
{
printf("incorrect dec1 decoupler flags! should be 'nnn' ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' ))
{
printf("incorrect dec2 decoupler flags! Should be 'nny' ");
psg_abort(1);
}
/* Phase incrementation for hypercomplex 2D data */
if (phase == 2)
{
tsadd(t1,1,4);
}
if (phase2 == 2)
{
tsadd(t2,1,4);
}
/* Set up f1180 half_dwell time (1/sw1)/2.0 */
tau1 = d2 - (4.0*pw/PI + 2.0*pwN);
if(f1180[A] == 'y')
{
tau1 += (1.0/(2.0*sw1));
}
if(tau1 < 0.2e-6) tau1 = 0.0;
tau1 = tau1/2.0;
/* Set up f2180 half dwell time (1/sw2)/2.0 */
tau2 = d3;
if(f2180[A] == 'y')
{
tau2 += (1.0/(2.0*sw2));
}
if(tau2 < 0.2e-6) tau2 = 0.0;
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2 ;
t1_counter = (int)((d2-d2_init)*sw1 + 0.5);
if(t1_counter % 2)
{
tsadd(t1,2,4);
tsadd(t10,2,4);
}
if( ix == 1) d3_init = d3 ;
t2_counter = (int)((d3-d3_init)*sw2 + 0.5);
if(t2_counter % 2)
{
tsadd(t2,2,4);
tsadd(t10,2,4);
}
if (flipphase < -0.01) flipphase = flipphase + 360.0;
initval(flipphase, v10);
obsstepsize(0.25);
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
dec2power(pwNlvl);
txphase(zero);
dec2phase(zero);
delay(d1);
if(gt1 > 0.2e-6)
{
dec2rgpulse(pwN, zero, 0.2e-6, 0.0);
delay(2.0e-6);
zgradpulse(gzlvl1, gt1);
delay(0.001);
}
rcvroff();
status(B);
rgpulse(pw, zero,rof1, 0.0);
delay(2.0e-6);
if (gt2 > 0.2e-6)
zgradpulse(gzlvl2,gt2);
delay(taua - gt2 - 2.0e-6);
sim3pulse(2.0*pw,(double)0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
delay(taua - gt2 - 400.0e-6);
if (gt2 > 0.2e-6)
{
zgradpulse(gzlvl2,gt2);
}
txphase(one);
dec2phase(t2);
delay(400.0e-6);
rgpulse(pw, one, 0.0, 0.0);
if (gt3 > 0.2e-6)
{
delay(2.0e-6);
zgradpulse(gzlvl3, gt3);
delay(200.0e-6);
}
txphase(zero);
dec2rgpulse(pwN, t2, 0.0, 0.0);
if (ref_flg[A] == 'y')
{
delay(tau2);
rgpulse(2.0*pw, zero, 0.0, 0.0);
dec2phase(t3);
if (gt5 > 0.2e-6)
{
delay(2.0*bigT - gt5 - 2.0*pw - 1.0e-3);
zgradpulse(gzlvl5, gt5);
delay(1.0e-3);
dec2rgpulse(2.0*pwN, t3, 0.0, 0.0);
delay(2.0e-6);
zgradpulse(gzlvl5, gt5);
delay(2.0*bigT - tau2 - gt5 - 2.0e-6);
}
else
{
delay(2.0*bigT - 2.0*pw);
dec2rgpulse(2.0*pwN, t3, 0.0, 0.0);
delay(2.0*bigT - tau2);
}
}
else
{
dec2phase(zero);
if (gt4 > 0.2e-6)
{
delay(2.0e-6);
zgradpulse(gzlvl4, gt4);
delay(bigT - gt4 - 2.0e-6);
sim3pulse(2.0*pw,(double)0.0, 2.0*pwN, zero,zero, zero, 0.0, 0.0);
delay(2.0e-6);
zgradpulse(gzlvl4, gt4);
delay(1.0e-3 - 2.0e-6);
}
else
{
delay(bigT);
sim3pulse(2.0*pw,(double)0.0, 2.0*pwN, zero,zero, zero, 0.0, 0.0);
delay(1.0e-3);
gt4 = 0.0;
}
zgradpulse(gzlvl5, gt5);
txphase(t1);
delay(bigT - gt4 - gt5 - 1.0e-3 - 2.0*GRADIENT_DELAY);
rgpulse(pw, t1, 0.0, 0.0);
delay(tau1);
dec2rgpulse(2.0*pwN, t3, 0.0, 0.0);
txphase(zero);
delay(tau1);
rgpulse(pw, zero, 0.0, 0.0);
delay(2.0e-6);
zgradpulse(gzlvl5, gt5);
dec2phase(t4);
if (gt6 > 0.2e-6)
{
delay(tau2 + 100.0e-6);
zgradpulse(gzlvl6, gt6);
delay(bigT - gt5 - gt6 - 100.0e-6 - 2.0*GRADIENT_DELAY);
sim3pulse(2.0*pw,(double)0.0, 2.0*pwN, zero,zero, t4, 0.0, 0.0);
delay(2.0e-6);
dec2phase(zero);
zgradpulse(gzlvl6, gt6);
delay(bigT - tau2 - gt6 - 2.0e-6);
}
else
{
delay(bigT + tau2 - gt5 - 2.0*GRADIENT_DELAY);
sim3pulse(2.0*pw,(double)0.0, 2.0*pwN, zero,zero, t4, 0.0, 0.0);
dec2phase(zero);
delay(bigT - tau2);
}
}
if (gt7 > 0.2e-6)
{
dec2rgpulse(pwN, zero, 0.0,2.0e-6);
zgradpulse(gzlvl7, gt7);
txphase(zero);
delay(200.0e-6);
if (pwHs > 0.2e-6)
{
xmtrphase(v10);
if (tpwrsf<4095.0) {obspower(tpwrs+6.0); obspwrf(tpwrsf);}
else obspower(tpwrs);
txphase(two);
shaped_pulse("H2Osinc", pwHs, two, 2.0e-6, 0.0);
xmtrphase(zero);
obspower(tpwr); obspwrf(4095.0);
}
rgpulse(pw, zero, 2.0e-6, 0.0);
}
else
{
sim3pulse(pw,(double)0.0, pwN, zero,zero, zero, 0.0, 0.0);
}
delay(2.0e-6);
if(mag_flg[A] == 'y')
{
magradpulse(gzcal*gzlvl8, gt8);
}
else
{
zgradpulse(gzlvl8, gt8);
}
if (flg_3919[A] == 'y')
{
delay(taub - 31.0*factor*pw - 2.5*tau_3919 - gt8 - 2.0e-6);
rgpulse(pw*factor*3.0, two, 0.0, 0.0);
delay(tau_3919);
rgpulse(pw*factor*9.0, two, 0.0, 0.0);
delay(tau_3919);
rgpulse(pw*factor*19.0, two, 0.0, 0.0);
delay(tau_3919/2.0 - pwN);
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
delay(tau_3919/2.0 - pwN);
rgpulse(pw*factor*19.0, zero, 0.0, 0.0);
delay(tau_3919);
rgpulse(pw*factor*9.0, zero, 0.0, 0.0);
delay(tau_3919);
rgpulse(pw*factor*3.0, zero, 0.0, 0.0);
delay(taub - 31.0*factor*pw - 2.5*tau_3919 - gt8 - POWER_DELAY - 402.0e-6);
}
else
{
if (tpwrsf<4095.0) {obspower(tpwrs+6.0); obspwrf(tpwrsf);}
else obspower(tpwrs);
txphase(two);
xmtrphase(v10);
delay(taub - pwHs - gt8 - 2.0*POWER_DELAY - 2.0e-6);
shaped_pulse("H2Osinc", pwHs, two, 0.0, 0.0);
obspower(tpwr); obspwrf(4095.0);
xmtrphase(zero);
txphase(zero);
sim3pulse(2.0*pw, (double)0.0, 2.0*pwN, zero, zero, zero, 2.0e-6, 0.0);
if (tpwrsf<4095.0) {obspower(tpwrs+6.0); obspwrf(tpwrsf);}
else obspower(tpwrs);
txphase(two);
xmtrphase(v10);
shaped_pulse("H2Osinc", pwHs, two, 2.0e-6, 0.0);
xmtrphase(zero);
obspower(tpwr); obspwrf(4095.0);
dec2power(dpwr2);
delay(taub - pwHs - gt8 - 3.0*POWER_DELAY - 402.0e-6);
}
dec2power(dpwr2);
if(mag_flg[A] == 'y')
{
magradpulse(gzcal*gzlvl8, gt8);
}
else
{
zgradpulse(gzlvl8, gt8);
}
delay(400.0e-6);
status(C);
setreceiver(t10);
rcvron();
}
pulsesequence()
{
double hsglvl = getval("hsglvl"),
hsgt = getval("hsgt"),
tau,
taug,
evolcorr,
pwx180 = getval("pwx180"),
pwxlvl180 = getval("pwxlvl180"),
pwx180r = getval("pwx180r"),
pwxlvl180r = getval("pwxlvl180r"),
mult = getval("mult"),
null = getval("null");
int phase1 = (int)(getval("phase")+0.5),
prgcycle = (int)(getval("prgcycle")+0.5),
ZZgsign;
char pwx180ad[MAXSTR],
pwx180adR[MAXSTR],
pwx180ref[MAXSTR];
getstr("pwx180ad", pwx180ad);
getstr("pwx180adR", pwx180adR);
getstr("pwx180ref", pwx180ref);
tau = 1/(4*(getval("j1xh")));
evolcorr = (4*pwx/PI) + 2*pw + 8.0e-6;
if (mult > 0.5)
taug = 2*tau + getval("tauC");
else
taug = 0.0;
ZZgsign=-1;
if (mult == 2) ZZgsign=1;
assign(ct,v17);
assign(zero,v18);
assign(zero,v19);
if (getflag("prgflg") && (satmode[0] == 'y') && (prgcycle > 1.5))
{
hlv(ct,v17);
mod2(ct,v18);
dbl(v18,v18);
if (prgcycle > 2.5)
{
hlv(v17,v17);
hlv(ct,v19);
mod2(v19,v19);
dbl(v19,v19);
}
}
settable(t1, 4, ph1);
settable(t2, 2, ph2);
settable(t3, 8, ph3);
settable(t4, 16, ph4);
settable(t5, 16, ph5);
getelem(t1, v17, v1);
getelem(t3, v17, v3);
getelem(t4, v17, v4);
getelem(t2, v17, v2);
getelem(t5, v17, oph);
assign(zero,v6);
add(oph,v18,oph);
add(oph,v19,oph);
if (phase1 == 2)
incr(v2);
/*
mod2(id2,v14);
dbl(v14, v14);
*/
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v14);
add(v2, v14, v2);
add(oph, v14, oph);
status(A);
obspower(tpwr);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
if (satmode[0] == 'y')
{
if ((d1-satdly) > 0.02)
delay(d1-satdly);
else
delay(0.02);
if (getflag("slpsat"))
{
shaped_satpulse("relaxD",satdly,zero);
if (getflag("prgflg"))
shaped_purge(v6,zero,v18,v19);
}
else
{
satpulse(satdly,zero,rof1,rof1);
if (getflag("prgflg"))
purge(v6,zero,v18,v19);
}
}
else
delay(d1);
if (getflag("wet"))
wet4(zero,one);
decpower(pwxlvl);
status(B);
if ((getflag("PFGflg")) && (getflag("nullflg")))
{
rgpulse(0.5 * pw, zero, rof1, rof1);
delay(2 * tau);
decpower(pwxlvl180);
decshaped_pulse(pwx180ad, pwx180, zero, rof1, rof1);
rgpulse(2.0 * pw, zero, rof1, rof1);
delay(2 * tau + 2 * POWER_DELAY);
decshaped_pulse(pwx180adR, pwx180, zero, rof1, rof1);
decpower(pwxlvl);
rgpulse(1.5 * pw, two, rof1, rof1);
zgradpulse(hsglvl, hsgt);
delay(1e-3);
}
else if (null != 0.0)
{
rgpulse(pw, zero, rof1, rof1);
delay(2 * tau);
decpower(pwxlvl180);
decshaped_pulse(pwx180ad, pwx180, zero, rof1, rof1);
rgpulse(2.0 * pw, zero, rof1, rof1);
delay(2 * tau + 2 * POWER_DELAY);
decshaped_pulse(pwx180adR, pwx180, zero, rof1, rof1);
decpower(pwxlvl);
rgpulse(pw, two, rof1, rof1);
if (satmode[1] == 'y')
{
if (getflag("slpsat"))
shaped_satpulse("BIRDnull",null,zero);
else
satpulse(null,zero,rof1,rof1);
}
else
delay(null);
}
rgpulse(pw, v6, rof1, rof1);
delay(tau);
decpower(pwxlvl180);
decshaped_pulse(pwx180ad, pwx180, zero, rof1, rof1);
rgpulse(2.0 * pw, zero, rof1, rof1);
delay(tau + 2 * POWER_DELAY);
decshaped_pulse(pwx180adR, pwx180, zero, rof1, rof1);
decpower(pwxlvl);
rgpulse(pw, v1, rof1, rof1);
if (getflag("PFGflg"))
{
zgradpulse(hsglvl, 2 * hsgt);
delay(1e-3);
}
decrgpulse(pwx, v2, rof1, 2.0e-6);
delay(d2/2);
rgpulse(2*pw,zero,2.0e-6,2.0e-6);
delay(d2/2);
if (mult>0.5)
{
delay(taug);
decpower(pwxlvl180r);
decshaped_pulse(pwx180ref, pwx180r, zero, rof1, rof1);
rgpulse(mult * pw, zero, rof1, rof1);
delay(taug - mult*pw - 2*rof1 + 2*POWER_DELAY + evolcorr);
decshaped_pulse(pwx180ref, pwx180r, zero, rof1, rof1);
}
else
{
decpower(pwxlvl180);
decshaped_pulse(pwx180ad, pwx180, zero, rof1, rof1);
delay(2*POWER_DELAY + evolcorr);
decshaped_pulse(pwx180ad, pwx180, zero, rof1, rof1);
}
decpower(pwxlvl);
decrgpulse(pwx, v4, 2.0e-6, rof1);
if (getflag("PFGflg"))
{
zgradpulse(ZZgsign*0.6 * hsglvl, 1.2 * hsgt);
delay(1e-3);
}
rgpulse(pw, v3, rof1, rof1);
decpower(pwxlvl180);
decshaped_pulse(pwx180adR, pwx180, zero, rof1, rof1);
decpower(dpwr);
delay(tau - 2*rof1 - (2*pw/PI));
rgpulse(2 * pw, zero, rof1, rof2);
decpower(pwxlvl180);
decshaped_pulse(pwx180ad, pwx180, zero, rof1, rof1);
decpower(dpwr);
delay(tau);
status(C);
}
void pulsesequence()
{
double j1min,
j1max,
pwxlvl,
pwx,
gzlvl0,
gt0,
gzlvl1,
gt1,
gzlvl3,
gt3,
bird,
gstab,
hsglvl,
hsgt,
satdly,
tauX,
tau,
tautau,
t1max,
tauA,
tauB,
tau1,
tau2,
taumb;
char sspul[MAXSTR],
accord[MAXSTR],
satmode[MAXSTR];
int ijscaleU,
ijscaleD,
t1_counter;
j1min = getval("j1min");
j1max = getval("j1max");
pwxlvl = getval("pwxlvl");
pwx = getval("pwx");
getstr("sspul",sspul);
getstr("accord",accord);
gzlvl0 = getval("gzlvl0");
gt0 = getval("gt0");
gzlvl1 = getval("gzlvl1");
gt1 = getval("gt1");
gzlvl3 = getval("gzlvl3");
gt3 = getval("gt3");
gstab = getval("gstab");
hsglvl = getval("hsglvl");
hsgt = getval("hsgt");
satdly = getval("satdly");
getstr("satmode",satmode);
tauX = 1/(2*(getval("jnmax")));
tauA = 1/(2*(j1min + 0.146*(j1max - j1min)));
tauB = 1/(2*(j1max - 0.146*(j1max - j1min)));
bird = (tauA+tauB)/2;
ijscaleD = (int)(getval("jscaleD") + 0.5);
ijscaleU = (int)(getval("jscaleU") + 0.5);
t1max = ((getval("ni")-1)/getval("sw1")) + 0.0005;
tautau = ((ijscaleU - 1)*d2);
taumb = 1/(2*(getval("jnmin")));
if(ix == 1)
d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*(getval("sw1")) + 0.5);
if (accord[0] == 'y')
{
if ((taumb - tauX) < t1max/2)
taumb = tauX + t1max/2;
tau = ((taumb - tauX)*t1_counter/ni);
}
else
{
taumb = 1/(2*(getval("jnxh")));
if (ijscaleU > 0)
tau = 0.0;
else
tau = t1max/2;
}
tau1 = ijscaleD * d2;
tau2 = (ijscaleD*t1max) - tau1;
if ((ijscaleD > 0) && (ijscaleU > 0))
{
printf("Either jscaleD or jscaleU must be set to zero.\n");
psg_abort(1);
}
settable(t1,1,ph1);
settable(t3,2,ph3);
settable(t4,1,ph4);
settable(t5,4,ph5);
settable(t6,4,ph6);
getelem(t3,ct,v3);
getelem(t6,ct,oph);
initval(2.0*(double)((int)(d2*getval("sw1")+0.5)%2),v10);
add(v3,v10,v3);
add(oph,v10,oph);
status(A);
decpower(pwxlvl);
if (sspul[0] == 'y')
{
zgradpulse(hsglvl*1.3,hsgt);
rgpulse(pw,zero,rof1,rof1);
zgradpulse(hsglvl*1.3,hsgt);
}
if (satmode[0] == 'y')
{
if (d1 - satdly > 0)
delay(d1 - satdly);
else
delay(0.02);
obspower(satpwr);
if (satfrq != tof)
obsoffset(satfrq);
rgpulse(satdly,zero,rof1,rof1);
if (satfrq != tof)
obsoffset(tof);
obspower(tpwr);
delay(1.0e-5);
}
else
{ delay(d1); }
if (getflag("wet"))
wet4(zero,one);
status(B);
rgpulse(pw,t1,rof1,rof2);
/* Start of J filter */
zgradpulse(gzlvl0/2,gt0);
delay(tauA - gt0);
decrgpulse(pwx, zero, rof1, rof1);
zgradpulse(-gzlvl0/3,gt0);
delay(tauB - gt0);
decrgpulse(pwx, zero, rof1, rof1);
zgradpulse(-gzlvl0/6,gt0);
delay(gstab);
/* End of J filter */
/* Start of STARR */
/* rev-BIRD in the middle of tau2 - 180 for C13-H and 360 for C12-H */
/* - decouple long-range CH */
/* evolution and let HH evolution continue except for */
/* 2-bond correlations */
delay(tau2/2);
zgradpulse(gzlvl0*1.5,gt0);
delay(gstab);
rgpulse(pw,zero,rof1,rof1);
delay(bird);
simpulse(2*pw,2*pwx,zero,zero,rof1,rof1);
delay(bird);
rgpulse(pw,zero,rof1,rof2);
zgradpulse(-gzlvl0*1.5,gt0);
delay(gstab);
delay(tau2/2);
/* Simple C 180 in the middle of tau1 - decouple */
/* CH evolution and let HH evolution continue */
/* AND Start of CT-VD */
if (!((accord[0] == 'n') && (ijscaleU == 1)))
{
delay(tau1/2 + tau/2 + tautau/4);
zgradpulse(gzlvl0,gt0);
delay(gstab);
decrgpulse(2*pwx,two,rof1,rof1);
zgradpulse(-gzlvl0,gt0);
delay(gstab);
delay(tau1/2 + tau/2 + tautau/4);
}
/* End of STARR */
if (accord[0] == 'y')
delay(taumb - tau);
else
delay(taumb);
/* End of CT-VD */
decrgpulse(pwx,v3,rof1,rof1);
zgradpulse(gzlvl1,gt1);
delay(gstab);
delay(d2/2);
rgpulse(pw*2.0,t4,rof1,rof1);
delay(d2/2);
zgradpulse(gzlvl1,gt1);
delay(gstab);
decrgpulse(pwx,t5,rof1,rof1);
zgradpulse(gzlvl3,gt3);
decpower(dpwr);
delay(t1max/2 + tautau/2);
status(C);
}
pulsesequence() {
// =========================================================
// Define Variables and Objects and Get Parameter Values
// =========================================================
// --------------------------------
// Acquisition Decoupling
// -------------------------------
char Hseq[MAXSTR];
getstr("Hseq",Hseq);
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
// ---------------------------------------------
// Determine taur, One Rotor Cycle and Set tHrr.
// ---------------------------------------------
double srate = getval("srate");
double taur = 0.0;
if (srate >= 500.0)
taur = roundoff((1.0/srate), 0.125e-6);
else {
abort_message("ABORT: Spin Rate (srate) must be greater than 500\n");
}
double tHrr = getval("nHrr")*taur;
double tHrrret= 2.0*tHrr;
putCmd("tHrrret = %f\n",tHrrret*1.0e6);
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
//------------------------------
// Dutycycle Protection
//------------------------------
DUTY d = init_dutycycle();
d.dutyon = getval("pwX90");
d.dutyoff = d1 + 4.0e-6;
if (getval("aHrr") > 0.0)
d.dutyon += 2.0*tHrr;
d.c1 = d.c1 + (!strcmp(Hseq,"tppm"));
d.c1 = d.c1 + ((!strcmp(Hseq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(Hseq,"spinal"));
d.c2 = d.c2 + ((!strcmp(Hseq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
//---------------------------
// Set Phase Tables
//---------------------------
settable(phX90,4,tblX90);
settable(phHrr,4,tblHrr);
settable(ph1Hrr,4,tblHrr2);
settable(phRec,4,tblRec);
setreceiver(phRec);
//===========================
// Begin Sequence
//===========================
txphase(phX90); decphase(zero);
obspwrf(getval("aX90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
//----------------------------
// X Direct Polarization
//----------------------------
rgpulse(getval("pwX90"),phX90,0.0,0.0);
obsunblank();
// -----------------------------
// Begin Rotary Resonance
// -----------------------------
if(getval("aHrr") > 0.0) {
obspwrf(getval("aHrr"));
txphase(phHrr);
xmtron();
delay(tHrr);
txphase(ph1Hrr);
delay(tHrr);
xmtroff();
}
//===========================
// Begin Acquisition
//===========================
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence()
{
double mixN = getval("mixN"),
gzlvlC = getval("gzlvlC"),
gtC = getval("gtC"),
gstab = getval("gstab"),
selpwrA = getval("selpwrA"),
selpwA = getval("selpwA"),
gzlvlA = getval("gzlvlA"),
gtA = getval("gtA"),
selpwrB = getval("selpwrB"),
selpwB = getval("selpwB"),
gzlvlB = getval("gzlvlB"),
gtB = getval("gtB"),
selfrq = getval("selfrq"),
zqfpw3 = getval("zqfpw3"),
zqfpwr3 = getval("zqfpwr3"),
gzlvlzq3 = getval("gzlvlzq3"),
mixNcorr,
sweeppw = getval("sweeppw"),
sweeppwr = getval("sweeppwr");
char sweepshp[MAXSTR],
selshapeA[MAXSTR],
selshapeB[MAXSTR],
zqfpat3[MAXSTR];
//synchronize gradients to srate for probetype='nano'
// Preserve gradient "area"
gtC = syncGradTime("gtC","gzlvlC",1.0);
gzlvlC = syncGradLvl("gtC","gzlvlC",1.0);
gtA = syncGradTime("gtA","gzlvlA",1.0);
gzlvlA = syncGradLvl("gtA","gzlvlA",1.0);
gtB = syncGradTime("gtB","gzlvlB",1.0);
gzlvlB = syncGradLvl("gtB","gzlvlB",1.0);
getstr("sweepshp",sweepshp);
getstr("selshapeA",selshapeA);
getstr("selshapeB",selshapeB);
getstr("zqfpat3",zqfpat3);
mixNcorr=0.0;
if (getflag("Gzqfilt"))
mixNcorr=getval("zqfpw3");
hlv(ct,v1); hlv(v1,v1); hlv(v1,v1); hlv(v1,v1); mod4(v1,v1);
mod4(ct,v2); add(v1,v2,v2);
hlv(ct,v3); hlv(v3,v3); mod4(v3,v3); add(v1,v3,v3); dbl(v3,v4);
dbl(v2,oph); add(oph,v4,oph); add(oph,v1,oph);
/* BEGIN THE ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
delay(5.0e-5);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
if (satmode[0] == 'y')
{
if ((d1-satdly) > 0.02)
delay(d1-satdly);
else
delay(0.02);
satpulse(satdly,zero,rof1,rof1);
}
else
delay(d1);
if (getflag("wet"))
wet4(zero,one);
status(B);
if (selfrq != tof)
obsoffset(selfrq);
rgpulse(pw, v1, rof1, rof1);
if (selfrq != tof)
obsoffset(selfrq);
zgradpulse(gzlvlA,gtA);
delay(gstab);
obspower(selpwrA);
shaped_pulse(selshapeA,selpwA,v2,rof1,rof1);
obspower(tpwr);
zgradpulse(gzlvlA,gtA);
delay(gstab);
if (selfrq != tof)
delay(2*OFFSET_DELAY);
zgradpulse(gzlvlB,gtB);
delay(gstab);
obspower(selpwrB);
shaped_pulse(selshapeB,selpwB,v3,rof1,rof1);
obspower(tpwr);
zgradpulse(gzlvlB,gtB);
delay(gstab);
if (selfrq != tof)
obsoffset(tof);
rgpulse(pw,v1,rof1,rof1);
obspower(sweeppwr);
delay(0.31*mixN);
zgradpulse(gzlvlC,gtC);
delay(gstab);
shaped_pulse(sweepshp,sweeppw,zero,rof1,rof1);
delay(gstab);
zgradpulse(-gzlvlC,gtC);
delay(0.49*mixN);
zgradpulse(-gzlvlC,2*gtC);
delay(gstab);
if (getflag("Gzqfilt"))
{
obspower(zqfpwr3);
rgradient('z',gzlvlzq3);
delay(100.0e-6);
shaped_pulse(zqfpat3,zqfpw3,zero,rof1,rof1);
delay(100.0e-6);
rgradient('z',0.0);
}
else
shaped_pulse(sweepshp,sweeppw,zero,rof1,rof1);
delay(gstab);
zgradpulse(gzlvlC,2*gtC);
delay(0.2*mixN);
obspower(tpwr);
rgpulse(pw,v1,rof1,rof2);
status(C);
}
pulsesequence()
{
double gzlvl1,qlvl,grise,gstab,gt1,taud2,tau1;
int icosel;
grise=getval("grise");
gstab=getval("gstab");
gt1=getval("gt1");
gzlvl1=getval("gzlvl1");
qlvl=getval("qlvl");
tau1 = getval("tau1");
taud2 = getval("taud2");
if (phase1 == 2)
{
icosel=-1;
if (ix==1)
fprintf(stdout,"P-type MQCOSY\n");
}
else
{
icosel=1; /* Default to N-type experiment */
if (ix==1)
fprintf(stdout,"N-type MQCOSY\n");
}
qlvl++ ;
status(A);
obspower(satpwr);
delay(d1);
rgpulse(satdly,zero,rof1,rof2);
obspower(tpwr);
delay(1.0e-6);
rgpulse(pw,oph,rof1,rof2);
delay(d2);
rgradient('z',gzlvl1);
delay(gt1+grise);
rgradient('z',0.0);
txphase(oph);
delay(grise);
rgpulse(pw,oph,0.0,rof2);
delay(taud2);
rgradient('z',gzlvl1);
delay(gt1+grise);
rgradient('z',0.0);
txphase(oph);
delay(grise);
delay(taud2);
status(B);
rgpulse(pw,oph,rof1,rof2);
delay(tau1);
rgradient('z',gzlvl1*qlvl*(double)icosel);
delay(gt1+grise);
rgradient('z',0.0);
delay(grise);
delay(gstab);
status(C);
}
pulsesequence()
{
char pshape[MAXSTR], httype[MAXSTR];
int Hdim = (int) (0.5 + getval("Hdim")); /* dimension for testing */
double rg1 = 2.0e-6,
compH = getval("compH"), /* compression factor for H-1 channel */
gt0 = getval("gt0"), /* gradient pulse preceding d1 */
gt1 = getval("gt1"), /* gradient pulse preceding mixing */
gt2 = getval("gt2"), /* gradient pulse following mixing */
gzlvl0 = getval("gzlvl0"), /* gradient level for gt1 */
gzlvl1 = getval("gzlvl1"), /* gradient level for gt2 */
gzlvl2 = getval("gzlvl2"), /* gradient level for gt3 */
gstab = getval("gstab"); /* delay for gradient recovery */
shape hdx;
getstr("pshape", pshape);
getstr("httype", httype);
add(one,oph,v1);
/* MAKE PBOX SHAPES */
if(Hdim == 0)
hdx = pboxHT(pshape, pw*compH, tpwr, httype[0]); /* HADAMARD stuff */
else /* if(Hdim == 1) */
hdx = pboxHT_F1(pshape, pw*compH, tpwr, httype[0]);
/* Optional stuff to enable manual power calibration */
if ((Hdim == 0) && (getval("htcal") > 0.5)) hdx.pwr = getval("htpwr");
if ((Hdim == 1) && (getval("htcal1") > 0.5)) hdx.pwr = getval("htpwr1");
/* THE PULSE PROGRAMM STARTS HERE */
status(A);
delay(1.0e-4);
zgradpulse(gzlvl0,gt0);
pre_sat();
if (getflag("wet"))
wet4(zero,one);
obspower(tpwr);
delay(1.0e-5);
status(B);
if ((httype[0] == 'i') || (httype[0] == 's')) /* inversion and sequential inversion */
{
pbox_pulse(&hdx, zero, rof1, rof1);
zgradpulse(gzlvl2,gt2);
obspower(tpwr);
delay(gstab);
rgpulse(pw,oph,rg1,rof2);
}
else if (httype[0] == 'r') /* gradient echo */
{
obspower(tpwr);
rgpulse(pw,oph,rg1,rg1);
zgradpulse(gzlvl1,gt1);
delay(gstab);
pbox_pulse(&hdx, oph, rg1, rof2);
zgradpulse(gzlvl1,gt1);
delay(gstab);
}
else /* if (httype[0] == 'e') */ /* selective excitation */
pbox_pulse(&hdx, oph, rof1, rof1);
status(C);
}
pulsesequence()
{
char codec[MAXSTR], codecseq[MAXSTR];
int icosel, t1_counter;
double d2_init = 0.0,
rf0 = 4095, rf200, pw200,
copwr, codmf, cores, copwrf,
tpwrs,
pwHs = getval("pwHs"),
compH = getval("compH"),
pwClvl = getval("pwClvl"),
pwC = getval("pwC"),
compC = getval("compC"),
pwNlvl = getval("pwNlvl"),
pwN = getval("pwN"),
sw1 = getval("sw1"),
swH = getval("swH"),
swC = getval("swC"),
swN = getval("swN"),
swTilt,
angle_H = getval("angle_H"),
angle_C = getval("angle_C"),
angle_N,
cos_H,
cos_C,
cos_N,
mix = getval("mix"),
tauCH = getval("tauCH"), /* 1/4JCH */
tauNH = getval("tauNH"), /* 1/4JNH */
tau1, tau2, tau3,
tofali =getval("tofali"), /* aliphatic protons offset */
dofcaco =getval("dofcaco"), /* offset for caco decoupling, ~120 ppm */
dof = getval("dof"),
gstab = getval("gstab"),
gt0 = getval("gt0"), gzlvl0 = getval("gzlvl0"),
gt1 = getval("gt1"), gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gt3 = getval("gt3"), gzlvl3 = getval("gzlvl3"),
gt4 = getval("gt4"), gzlvl4 = getval("gzlvl4"),
gt5 = getval("gt5"), gzlvl5 = getval("gzlvl5"),
gt6 = getval("gt6"), gzlvl6 = getval("gzlvl6"),
gt7 = getval("gt7"), gzlvl7 = getval("gzlvl7"),
gt8 = getval("gt8"), gzlvl8 = getval("gzlvl8"),
gt9 = getval("gt9"), gzlvl9 = getval("gzlvl9");
/* LOAD VARIABLES */
copwr = getval("copwr"); copwrf = getval("copwrf");
codmf = getval("codmf"); cores = getval("cores");
getstr("codecseq", codecseq); getstr("codec", codec);
/* Load phase cycling variables */
settable(t1, 4, phi1); settable(t2, 2, phi2); settable(t3, 1, phi3);
settable(t4, 8, phi4); settable(t5, 1, phi5); settable(t14, 2, phi14);
settable(t11, 8, rec);
angle_N=0.0; cos_N=0.0;
/* activate auto-calibration flags */
setautocal();
if (autocal[0] == 'n')
{
/* 180 degree adiabatic C13 pulse from 0 to 200 ppm */
pw200 = getval("pw200");
rf200 = (compC*4095.0*pwC*4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35));
rf200 = (int) (rf200 + 0.5);
if (1.0/(4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35)) < pwC )
{ text_error( " Not enough C13 RF. pwC must be %f usec or less.\n",
(1.0e6/(4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35))) );
psg_abort(1); }
}
else /* if autocal = 'y'(yes), 'q'(quiet), r(read), or 's'(semi) */
{
strcpy(codecseq,"Pdec_154p");
if(FIRST_FID) /* call Pbox */
{
ppm = getval("dfrq");
bw = 200.0*ppm; pws = 0.001; ofs = 0.0; nst = 1000;
/* 1 ms long pulse, nst: number of steps */
stC200 = pbox_makeA("stC200", "sech", bw, pws, ofs, compC*pwC, pwClvl, nst);
bw=20.0*ppm; ofs=154*ppm;
Pdec_154p = pbox_Dsh("Pdec_154p", "WURST2", bw, ofs, compC*pwC, pwClvl);
}
rf200 = stC200.pwrf; pw200 = stC200.pw;
}
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69));
tpwrs = (int)(tpwrs+0.5);
/* check validity of parameter range */
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y'))
{ printf("incorrect Dec1 decoupler flags! "); psg_abort(1); }
if((dm2[A] == 'y' || dm2[B] == 'y'))
{ printf("incorrect Dec2 decoupler flags! "); psg_abort(1); }
if ((dpwr > 48) || (dpwr2 > 48))
{ printf("don't fry the probe, dpwr too high! "); psg_abort(1); }
/* set up angles for PR42 experiments */
/* sw1 is used as symbolic index */
if ( sw1 < 1000 ) { printf ("Please set sw1 to some value larger than 1000.\n"); psg_abort(1); }
if (angle_H < 0 || angle_C < 0 || angle_H > 90 || angle_C > 90 )
{ printf("angles must be set between 0 and 90 degree.\n"); psg_abort(1); }
cos_H = cos (PI*angle_H/180); cos_C = cos (PI*angle_C/180);
if ( (cos_H*cos_H + cos_C*cos_C) > 1.0) { printf ("Impossible angle combinations.\n"); psg_abort(1); }
else { cos_N = sqrt(1 - (cos_H*cos_H + cos_C*cos_C) ); angle_N = acos(cos_N)*180/PI; }
if (ix == 1) d2_init = d2;
t1_counter = (int)((d2-d2_init)*sw1 + 0.5);
if(t1_counter % 2) { tsadd(t3,2,4); tsadd(t11,2,4); }
swTilt = swH*cos_H + swC*cos_C + swN*cos_N;
if (phase1 == 1) {;} /* CC */
else if (phase1 == 2) { tsadd(t1, 1, 4); } /* SC */
else if (phase1 == 3) { tsadd(t2, 1, 4); tsadd(t14,1,4); } /* CS */
else if (phase1 == 4) { tsadd(t1, 1, 4); tsadd(t2,1,4); tsadd(t14,1,4); } /* SS */
if ( phase2 == 1 ) { tsadd(t5,2,4); icosel = 1; }
else icosel = -1;
tau1 = 1.0 * t1_counter * cos_H / swTilt;
tau2 = 1.0 * t1_counter * cos_C / swTilt;
tau3 = 1.0 * t1_counter * cos_N / swTilt;
tau1 = tau1/2.0; tau2 = tau2/2.0; tau3 =tau3/2.0;
if (ix ==1 )
{
printf ("Current Spectral Width:\t\t%5.2f\n", swTilt);
printf ("Angle_H: %5.2f \n", angle_H);
printf ("Angle_C: %5.2f \n", angle_C);
printf ("Angle_N: %5.2f \n", angle_N);
printf ("\n\n\n\n\n");
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
delay(d1);
rcvroff();
obsoffset(tofali); obspower(tpwr); obspwrf(4095.0);
decoffset(dof); decpower(pwClvl); decpwrf(rf0);
dec2offset(dof2); dec2power(pwNlvl); dec2pwrf(4095.0);
if (gt0 > 0.2e-6)
{
decrgpulse(pwC,zero,10.0e-6,0.0);
dec2rgpulse(pwN,zero,2.0e-6,2.0e-6);
zgradpulse(gzlvl0,gt0);
delay(gstab);
}
txphase(t1); decphase(t2); dec2phase(zero);
status(B);
rgpulse(pw,t1,4.0e-6,2.0e-6);
zgradpulse(gzlvl3,gt3);
delay(2.0*tauCH - gt3 - 2.0*GRADIENT_DELAY -4.0e-6);
decrgpulse(pwC,t2,2.0e-6,2.0e-6);
decphase(zero);
/*======= Start of c13 evolution ==========*/
if ( ((tau2 -PRG_START_DELAY - POWER_DELAY -pwN - 2.0*pwC/PI -2.0e-6)> 0)
&& ((tau2 -PRG_STOP_DELAY - POWER_DELAY - pwN - 2.0*pwC/PI -2.0e-6)>0)
&& (codec[A] == 'y') )
{
decpower(copwr); decpwrf(copwrf);
decprgon(codecseq,1/codmf,cores);
decon();
delay(tau2 -PRG_START_DELAY - POWER_DELAY -pwN - 2.0*pwC/PI -2.0e-6);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, t1, zero, zero, 0.0, 0.0);
delay(tau2 -PRG_STOP_DELAY - POWER_DELAY - pwN - 2.0*pwC/PI -2.0e-6);
decoff();
decprgoff();
}
else if ( (tau2 -pwN - 2.0*pwC/PI -2.0e-6) > 0)
{
delay(tau2 -pwN - 2.0*pwC/PI -2.0e-6);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, t1, zero, zero, 0.0, 0.0);
delay(tau2 -pwN - 2.0*pwC/PI -2.0e-6);
}
else
{
delay(2.0*tau2);
decphase(t14);
delay(4.0e-6);
sim3pulse(2.0*pw, 2.0*pwC, 2.0*pwN, t1, t14, zero, 0.0, 0.0);
delay(4.0e-6);
}
decpower(pwClvl);
decpwrf(rf0);
decphase(zero);
/*======= End of c13 evolution ==========*/
decrgpulse(pwC,zero, 2.0e-6,2.0e-6);
txphase(zero);
delay(2.0*tauCH + tau1 - gt3 - 4.0*pwC
- gstab -4.0e-6 - 2.0*GRADIENT_DELAY);
zgradpulse(gzlvl3,gt3);
delay(gstab);
decrgpulse(pwC,zero,0.0, 0.0);
decphase(one);
decrgpulse(2.0*pwC, one, 0.2e-6, 0.0);
decphase(zero);
decrgpulse(pwC, zero, 0.2e-6, 0.0);
delay(tau1);
rgpulse(pw,zero,2.0e-6,0.0);
/* ===========Beginning of NOE transfer ======= */
delay(mix - gt4 - gt5 - pwN -pwC - 2.0*gstab);
obsoffset(tof);
zgradpulse(gzlvl4,gt4);
delay(gstab);
decrgpulse(pwC, zero, 2.0e-6, 2.0e-6);
dec2rgpulse(pwN, zero, 2.0e-6, 2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(gstab);
/* H2O relaxes back to +z */
/* =========== End of NOE transfer ======= */
rgpulse(pw, zero, 2.0e-6, 2.0e-6);
zgradpulse(gzlvl6,gt6);
delay(tauNH - gt6 - 4.0e-6 - 2.0*GRADIENT_DELAY);
sim3pulse(2.0*pw, 0.0, 2*pwN, zero, zero, zero, 2.0e-6, 2.0e-6);
delay(tauNH - gt6 - gstab -4.0e-6 - 2.0*GRADIENT_DELAY);
zgradpulse(gzlvl6,gt6);
txphase(one);
delay(gstab);
rgpulse(pw,one,2.0e-6,2.0e-6);
txphase(two);
obspower(tpwrs);
shaped_pulse("H2Osinc", pwHs, two, 2.0e-6, 2.0e-6);
obspower(tpwr);
zgradpulse(gzlvl7,gt7);
dec2phase(t3);
decoffset(dofcaco); /* offset on 120ppm for CaCO decoupling */
decpwrf(rf200); /* fine power for stC200 */
delay(gstab);
dec2rgpulse(pwN,t3,2.0e-6,2.0e-6);
dec2phase(t4);
delay(tau3);
rgpulse(2.0*pw, zero, 2.0e-6, 2.0e-6);
decshaped_pulse("stC200", 1.0e-3, zero, 2.0e-6, 2.0e-6);
delay(tau3);
delay(gt1 +gstab +8.0e-6 - 1.0e-3 - 2.0*pw);
dec2rgpulse(2.0*pwN, t4, 2.0e-6, 2.0e-6);
dec2phase(t5);
zgradpulse(gzlvl1, gt1);
delay(gstab + WFG_START_DELAY + WFG_STOP_DELAY - 2.0*GRADIENT_DELAY);
sim3pulse(pw, 0.0, pwN, zero, zero, t5, 2.0e-6, 2.0e-6);
dec2phase(zero);
zgradpulse(gzlvl8, gt8);
delay(tauNH - gt8 - 2.0*GRADIENT_DELAY -4.0e-6);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 2.0e-6, 2.0e-6);
delay(tauNH - gt8 - gstab -4.0e-6 - 2.0*GRADIENT_DELAY);
zgradpulse(gzlvl8, gt8);
txphase(one); dec2phase(one);
delay(gstab);
sim3pulse(pw, 0.0, pwN, one, zero, one, 2.0e-6, 2.0e-6);
txphase(zero); dec2phase(zero);
zgradpulse(gzlvl9, gt9);
delay(tauNH - gt9 - 2.0*GRADIENT_DELAY -4.0e-6);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 2.0e-6, 2.0e-6);
delay(tauNH - gt9 - 2.0*GRADIENT_DELAY -gstab -4.0e-6);
zgradpulse(gzlvl9, gt9);
delay(gstab);
rgpulse(pw, zero, 2.0e-6, 2.0e-6);
delay((gt1/10.0) +gstab -2.0e-6 - 0.5*pw + 2.0*GRADIENT_DELAY + POWER_DELAY);
rgpulse(2.0*pw, zero, 2.0e-6, 2.0e-6);
zgradpulse(icosel*gzlvl2, gt1/10.0);
dec2power(dpwr2);
delay(gstab);
status(C);
setreceiver(t11);
}
pulsesequence()
{
/* DECLARE VARIABLES */
char autocal[MAXSTR], /* auto-calibration flag */
fsat[MAXSTR],
fscuba[MAXSTR],
f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
ddseq[MAXSTR], /* deuterium decoupling sequence */
shp_sl[MAXSTR],
shcreb[MAXSTR], /* reburp shape for center of t1 period */
shcgcob[MAXSTR], /* g3 inversion at 154 ppm (350 us) */
shcgcoib[MAXSTR], /* g3 time inversion at 154 ppm (350 us) */
shca180[MAXSTR], /* Ca 180 [D/sq(3)] during 15N CT */
shco180[MAXSTR], /* Co 180 [D/sq(15)] during 15N CT */
sel_flg[MAXSTR], /* active/passive purging of undesired
component */
fCT[MAXSTR], /* Flag for constant time C13 evolution */
fc180[MAXSTR],
cal_sphase[MAXSTR],
shared_CT[MAXSTR],
nietl_flg[MAXSTR];
int phase, phase2, ni2, icosel,
t1_counter, /* used for states tppi in t1 */
t2_counter; /* used for states tppi in t2 */
double tau1, /* t1 delay */
tau2, /* t2 delay */
taua, /* ~ 1/4JNH = 2.25 ms */
del1, /* time for C'-N to refocus set to 0.5*24.0 ms */
bigTN, /* nitrogen T period */
bigTC, /* carbon T period */
zeta, /* delay for transfer from ca to cb = 3.5 ms */
tsatpwr, /* low level 1H trans.power for presat */
sw1, /* sweep width in f1 */
sw2, /* sweep width in f2 */
tauf, /* 1/2J NH value */
pw_sl, /* selective pulse on water */
phase_sl, /* phase on water */
tpwrsl, /* power for pw_sl */
at,
d_cgcob, /* power level for g3 pulses at 154 ppm */
d_creb, /* power level for reburp 180 at center of t1 */
pwcgcob, /* g3 ~ 35o us 180 pulse */
pwcreb, /* reburp ~ 400us 180 pulse */
pwD, /* 2H 90 pulse, about 125 us */
pwDlvl, /* 2H 90 pulse, about 125 us */
pwca180, /* Ca 180 during N CT at d_ca180 */
pwco180, /* Co 180 during N CT at d_co180 */
d_ca180,
d_co180,
compC = getval("compC"), /* C-13 RF calibration parameters */
pwC = getval("pwC"),
pwClvl = getval("pwClvl"),
pwN,
pwNlvl,
sphase,
pw_sl1,
tpwrsl1,
gstab,
gt1,
gt2,
gt3,
gt4,
gt5,
gt6,
gt7,
gt8,
gt9,
gt11,
gt13,
gt14,
gzlvl1,
gzlvl2,
gzlvl3,
gzlvl4,
gzlvl5,
gzlvl6,
gzlvl7,
gzlvl8,
gzlvl9,
gzlvl11,
gzlvl13,
gzlvl14;
/* variables commented out are already defined by the system */
/* LOAD VARIABLES */
getstr("autocal",autocal);
getstr("fsat",fsat);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("fscuba",fscuba);
getstr("ddseq",ddseq);
getstr("shp_sl",shp_sl);
getstr("sel_flg",sel_flg);
getstr("fCT",fCT);
getstr("fc180",fc180);
getstr("cal_sphase",cal_sphase);
getstr("shared_CT",shared_CT);
getstr("nietl_flg",nietl_flg);
taua = getval("taua");
del1 = getval("del1");
bigTN = getval("bigTN");
bigTC = getval("bigTC");
zeta = getval("zeta");
pwN = getval("pwN");
tpwr = getval("tpwr");
tsatpwr = getval("tsatpwr");
dpwr = getval("dpwr");
pwNlvl = getval("pwNlvl");
pwD = getval("pwD");
pwDlvl = getval("pwDlvl");
phase = (int) ( getval("phase") + 0.5);
phase2 = (int) ( getval("phase2") + 0.5);
sw1 = getval("sw1");
sw2 = getval("sw2");
ni2 = getval("ni2");
tauf = getval("tauf");
pw_sl = getval("pw_sl");
phase_sl = getval("phase_sl");
tpwrsl = getval("tpwrsl");
at = getval("at");
sphase = getval("sphase");
pw_sl1 = getval("pw_sl1");
tpwrsl1 = getval("tpwrsl1");
gstab = getval("gstab");
gt1 = getval("gt1");
if (getval("gt2") > 0) gt2=getval("gt2");
else gt2=gt1*0.1;
gt3 = getval("gt3");
gt4 = getval("gt4");
gt5 = getval("gt5");
gt6 = getval("gt6");
gt7 = getval("gt7");
gt8 = getval("gt8");
gt9 = getval("gt9");
gt11 = getval("gt11");
gt13 = getval("gt13");
gt14 = getval("gt14");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl3 = getval("gzlvl3");
gzlvl4 = getval("gzlvl4");
gzlvl5 = getval("gzlvl5");
gzlvl6 = getval("gzlvl6");
gzlvl7 = getval("gzlvl7");
gzlvl8 = getval("gzlvl8");
gzlvl9 = getval("gzlvl9");
gzlvl11 = getval("gzlvl11");
gzlvl13 = getval("gzlvl13");
gzlvl14 = getval("gzlvl14");
if(autocal[0]=='n')
{
getstr("shcgcob",shcgcob);
getstr("shcgcoib",shcgcoib);
getstr("shcreb",shcreb);
getstr("shca180",shca180);
getstr("shco180",shco180);
d_ca180 = getval("d_ca180");
d_co180 = getval("d_co180");
d_cgcob = getval("d_cgcob");
d_creb = getval("d_creb");
pwca180 = getval("pwca180");
pwco180 = getval("pwco180");
pwcgcob = getval("pwcgcob");
pwcreb = getval("pwcreb");
}
else
{
strcpy(shcgcob,"Pg3_107p");
strcpy(shcgcoib,"Pg3i_107p");
strcpy(shcreb,"Preb_on");
strcpy(shca180,"Phard_15p");
strcpy(shco180,"Phard_133p");
if (FIRST_FID)
{
cgcob = pbox(shcgcob, G3CGCOB, CAB180ps, dfrq, compC*pwC, pwClvl);
cgcoib = pbox(shcgcoib, G3CGCOBi, CAB180ps, dfrq, compC*pwC, pwClvl);
creb = pbox(shcreb, CREB180, CAB180ps, dfrq, compC*pwC, pwClvl);
ca180 = pbox(shca180, CA180, CA180ps, dfrq, compC*pwC, pwClvl);
co180 = pbox(shco180, CO180, CA180ps, dfrq, compC*pwC, pwClvl);
}
d_ca180 = ca180.pwr;
d_co180 = co180.pwr;
d_cgcob = cgcob.pwr;
d_creb = creb.pwr;
pwca180 = ca180.pw;
pwco180 = co180.pw;
pwcgcob = cgcob.pw;
pwcreb = creb.pw;
}
/* LOAD PHASE TABLE */
settable(t1,2,phi1);
settable(t2,4,phi2);
settable(t3,8,phi3);
settable(t4,1,phi4);
settable(t5,16,phi5);
settable(t6,8,phi6);
settable(t7,1,phi7);
settable(t8,16,rec);
/* CHECK VALIDITY OF PARAMETER RANGES */
if(shared_CT[A] == 'n')
if(bigTN - 0.5*(ni2 -1)/sw2 - POWER_DELAY < 0.2e-6)
{
text_error(" ni2 is too big\n");
text_error(" please set ni2 smaller or equal to %d\n",
(int) ((bigTN -POWER_DELAY)*sw2*2.0) +1 );
psg_abort(1);
}
if(fCT[A] == 'y')
if(bigTC - 0.5*(ni-1)/sw1 - WFG_STOP_DELAY - gt14 - 102.0e-6
- POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY - PRG_START_DELAY
- POWER_DELAY - WFG_START_DELAY - 4.0e-6 - pwcgcob - WFG_STOP_DELAY
- POWER_DELAY - 4.0e-6 < 0.2e-6) {
text_error("ni is too big\n");
text_error(" please set ni smaller or equal to %d\n",
(int) ((bigTC - WFG_STOP_DELAY - gt14 - 102.0e-6
- POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY - PRG_START_DELAY
- POWER_DELAY - WFG_START_DELAY - 4.0e-6 - pwcgcob - WFG_STOP_DELAY
- POWER_DELAY - 4.0e-6)*sw1*2.0) +1 );
psg_abort(1);
}
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y'))
{
text_error("incorrect dec1 decoupler flags! ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y'))
{
text_error("incorrect dec2 decoupler flags! Should be 'nnn' ");
psg_abort(1);
}
if( tsatpwr > 6 )
{
text_error("TSATPWR too large !!! ");
psg_abort(1);
}
if( dpwr > 46 )
{
text_error("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if( dpwr2 > 47 )
{
text_error("don't fry the probe, DPWR2 too large! ");
psg_abort(1);
}
if( pwClvl > 63 )
{
text_error("don't fry the probe, pwClvl too large! ");
psg_abort(1);
}
if( pwNlvl > 63 )
{
text_error("don't fry the probe, pwNlvl too large! ");
psg_abort(1);
}
if( pw > 200.0e-6 )
{
text_error("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwN > 200.0e-6 )
{
text_error("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
if( pwC > 200.0e-6 )
{
text_error("dont fry the probe, pwC too high ! ");
psg_abort(1);
}
if( f1180[A] != 'n' && f2180[A] != 'n' ) {
text_error("flags may be set wrong: set f1180=n and f2180=n for 3d\n");
psg_abort(1);
}
if(d_ca180 > 58)
{
text_error("dont fry the probe, d_ca180 too high ! ");
psg_abort(1);
}
if(d_co180 > 58)
{
text_error("dont fry the probe, d_ca180 too high ! ");
psg_abort(1);
}
if( gt1 > 15e-3 || gt2 > 15e-3 || gt3 > 15e-3
|| gt4 > 15e-3 || gt5 > 15e-3 || gt6 > 15e-3
|| gt7 > 15e-3 || gt8 > 15e-3 || gt9 > 15e-3
|| gt11 > 15e-3 || gt13 > 15e-3
|| gt14 > 15e-3)
{
text_error("gti values must be < 15e-3\n");
psg_abort(1);
}
if(tpwrsl > 25) {
text_error("tpwrsl must be less than 25\n");
psg_abort(1);
}
if(tpwrsl1 > 25) {
text_error("tpwrsl1 must be less than 25\n");
psg_abort(1);
}
if( dpwr3 > 50) {
text_error("dpwr3 too high\n");
psg_abort(1);
}
if( del1 > 0.1 ) {
text_error("too long del1\n");
psg_abort(1);
}
if( zeta > 0.1 ) {
text_error("too long zeta\n");
psg_abort(1);
}
if( bigTN > 0.1) {
text_error("too long bigTN\n");
psg_abort(1);
}
if( bigTC > 0.1) {
text_error("too long bigTC\n");
psg_abort(1);
}
if( pw_sl > 10e-3) {
text_error("too long pw_sl\n");
psg_abort(1);
}
if( pw_sl1 > 10e-3) {
text_error("too long pw_sl1\n");
psg_abort(1);
}
if( at > 0.1 && dm2[D] == 'y') {
text_error("too long at with dec2\n");
psg_abort(1);
}
if(pwDlvl > 59) {
text_error("pwDlvl is too high; <= 59\n");
psg_abort(1);
}
if(d_creb > 62) {
text_error("d_creb is too high; <= 62\n");
psg_abort(1);
}
if(d_cgcob > 60) {
text_error("d_cgcob is too high; <=60\n");
psg_abort(1);
}
if(cal_sphase[A] == 'y') {
text_error("Use only to calibrate sphase\n");
text_error("Set zeta to 600 us, gt11=gt13=0, fCT=y, fc180=n\n");
}
if(nietl_flg[A] == 'y' && sel_flg[A] == 'y') {
text_error("Both nietl_flg and sel_flg cannot by y\n");
psg_abort(1);
}
if (fCT[A] == 'n' && fc180[A] =='y' && ni > 1.0) {
text_error("must set fc180='n' to allow Calfa/Cbeta evolution (ni>1)\n");
psg_abort(1);
}
/* Phase incrementation for hypercomplex 2D data */
/* changed from 1 to 3; spect. rev. not needed */
if (phase == 2) { tsadd(t2,3,4); tsadd(t3,3,4); }
if (shared_CT[A] == 'n')
{
if (phase2 == 2) { tsadd(t7,2,4); icosel = 1; }
else icosel = -1;
}
else
{
if (phase2 == 2) { tsadd(t7,2,4); icosel = -1; }
else icosel = 1;
}
if (nietl_flg[A] == 'y') icosel = -1*icosel;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2 ;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2) {
tsadd(t2,2,4);
tsadd(t8,2,4);
}
if( ix == 1) d3_init = d3 ;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2) {
tsadd(t5,2,4);
tsadd(t8,2,4);
}
/* Set up f1180 tau1 = t1 */
tau1 = d2;
if(f1180[A] == 'y' && fCT[A] == 'y')
tau1 += ( 1.0 / (2.0*sw1) );
if(f1180[A] == 'y' && fCT[A] == 'n')
tau1 += (1.0 / (2.0*sw1) - 4.0/PI*pwC - POWER_DELAY
- 4.0e-6);
if(f1180[A] == 'n' && fCT[A] == 'n')
tau1 = (tau1 - 4.0/PI*pwC - POWER_DELAY
- 4.0e-6);
if(tau1 < 0.2e-6) tau1 = 4.0e-7;
tau1 = tau1/2.0;
/* Set up f2180 tau2 = t2 */
tau2 = d3;
if(f2180[A] == 'y') {
tau2 += ( 1.0 / (2.0*sw2) );
if(tau2 < 0.2e-6) tau2 = 0.2e-6;
}
tau2 = tau2/2.0;
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tsatpwr); /* Set transmitter power for 1H presaturation */
decpower(pwClvl); /* Set Dec1 power to high power */
dec2power(pwNlvl); /* Set Dec2 power for 15N hard pulses */
dec3power(pwDlvl); /* Set Dec3 for 2H hard pulses */
/* Presaturation Period */
if (fsat[0] == 'y')
{
delay(2.0e-5);
rgpulse(d1,zero,0.0,2.0e-6);
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
delay(2.0e-5);
if (fscuba[0] == 'y')
{
delay(2.2e-2);
rgpulse(pw,zero,2.0e-6,0.0);
rgpulse(2*pw,one,2.0e-6,0.0);
rgpulse(pw,zero,2.0e-6,0.0);
delay(2.2e-2);
}
}
else
{
delay(d1);
}
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
txphase(zero);
dec2phase(zero);
delay(1.0e-5);
/* Begin Pulses */
status(B);
rcvroff();
lk_hold();
delay(20.0e-6);
rgpulse(pw,zero,0.0,0.0); /* 90 deg 1H pulse */
delay(0.2e-6);
zgradpulse(gzlvl5,gt5);
delay(2.0e-6);
delay(taua - gt5 - 2.2e-6); /* taua <= 1/4JNH */
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
dec2phase(t1); decphase(zero);
delay(taua - gt5 - 200.2e-6);
delay(0.2e-6);
zgradpulse(gzlvl5,gt5);
delay(200.0e-6);
if (sel_flg[A] == 'y')
{
rgpulse(pw,one,4.0e-6,0.0);
initval(1.0,v2);
obsstepsize(phase_sl);
xmtrphase(v2);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shp_sl,pw_sl,two,2.0e-6,0.0);
xmtrphase(zero);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
initval(1.0,v6);
dec2stepsize(45.0);
dcplr2phase(v6);
delay(0.2e-6);
zgradpulse(gzlvl3,gt3);
delay(200.0e-6);
dec2rgpulse(pwN,t1,0.0,0.0);
dcplr2phase(zero);
delay(1.34e-3 - SAPS_DELAY);
rgpulse(pw,zero,0.0,0.0);
rgpulse(2.0*pw,one,2.0e-6,0.0);
rgpulse(pw,zero,2.0e-6,0.0);
decpower(d_ca180);
dec2phase(zero);
delay(del1 - 1.34e-3 - 4.0*pw - 4.0e-6
- POWER_DELAY + WFG_START_DELAY + pwca180 + WFG_STOP_DELAY);
}
else
{
rgpulse(pw,three,4.0e-6,0.0);
initval(1.0,v2);
obsstepsize(phase_sl);
xmtrphase(v2);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shp_sl,pw_sl,zero,2.0e-6,0.0);
xmtrphase(zero);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
delay(0.2e-6);
zgradpulse(gzlvl3,gt3);
delay(200.0e-6);
dec2rgpulse(pwN,t1,0.0,0.0);
dec2phase(zero);
decpower(d_ca180);
delay(del1 - POWER_DELAY + WFG_START_DELAY
+ pwca180 + WFG_STOP_DELAY);
}
decphase(zero);
dec2rgpulse(2*pwN,zero,0.0,0.0);
decshaped_pulse(shca180,pwca180,zero,0.0,0.0);
dec2phase(one);
delay(del1);
dec2rgpulse(pwN,one,0.0,0.0);
decpower(pwClvl);
decphase(t2);
delay(0.2e-6);
zgradpulse(gzlvl4,gt4);
delay(200.0e-6);
dec2phase(t5);
/* Turn on D decoupling using the third decoupler */
dec3phase(one);
dec3power(pwDlvl);
dec3rgpulse(pwD,one,4.0e-6,0.0);
dec3phase(zero);
dec3power(dpwr3);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
/* Turn on D decoupling */
decrgpulse(pwC,t2,0.0,0.0);
delay(zeta
- PRG_STOP_DELAY - DELAY_BLANK - POWER_DELAY - 4.0e-6
- pwD
- gt11 - 102.0e-6 - POWER_DELAY - WFG_START_DELAY);
/* Turn off D decoupling */
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3blank();
dec3phase(three);
dec3power(pwDlvl);
dec3rgpulse(pwD,three,4.0e-6,0.0);
/* Turn off D decoupling */
decphase(zero);
delay(2.0e-6);
zgradpulse(gzlvl11,gt11);
delay(100.0e-6);
if (cal_sphase[A] == 'y')
{
decpower(pwClvl);
decshaped_pulse("hard",2.0*pwC,zero,4.0e-6,4.0e-6);
}
else
{
initval(1.0,v3);
decstepsize(sphase);
dcplrphase(v3);
decpower(d_creb);
decshaped_pulse(shcreb,pwcreb,zero,4.0e-6,4.0e-6);
dcplrphase(zero);
}
delay(2.0e-6);
zgradpulse(gzlvl11,gt11);
delay(100.0e-6);
/* Turn on D decoupling using the third decoupler */
dec3phase(one);
dec3power(pwDlvl);
dec3rgpulse(pwD,one,4.0e-6,0.0);
dec3phase(zero);
dec3power(dpwr3);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
/* Turn on D decoupling */
delay(zeta - WFG_STOP_DELAY - gt11 - 102.0e-6
- POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY - PRG_START_DELAY
- DELAY_BLANK - POWER_DELAY - 4.0e-6);
decpower(pwClvl);
decrgpulse(pwC,t3,4.0e-6,0.0);
if (fCT[A] == 'y')
{
delay(tau1);
decpower(d_cgcob);
decshaped_pulse(shcgcob,pwcgcob,zero,4.0e-6,0.0);
delay(bigTC - POWER_DELAY - WFG_START_DELAY - 4.0e-6
- pwcgcob - WFG_STOP_DELAY
- 102.0e-6 - gt14
- PRG_STOP_DELAY - DELAY_BLANK
- POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY - WFG_START_DELAY);
/* Turn off D decoupling */
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3blank();
dec3phase(three);
dec3power(pwDlvl);
dec3rgpulse(pwD,three,4.0e-6,0.0);
/* Turn off D decoupling */
delay(2.0e-6);
zgradpulse(gzlvl14,gt14);
delay(100.0e-6);
initval(1.0,v4);
decstepsize(sphase);
dcplrphase(v4);
decpower(d_creb);
decshaped_pulse(shcreb,pwcreb,zero,4.0e-6,4.0e-6);
dcplrphase(zero);
delay(2.0e-6);
zgradpulse(gzlvl14,gt14);
delay(100.0e-6);
/* Turn on D decoupling using the third decoupler */
dec3phase(one);
dec3power(pwDlvl);
dec3rgpulse(pwD,one,4.0e-6,0.0);
dec3phase(zero);
dec3power(dpwr3);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
/* Turn on D decoupling */
delay(bigTC - tau1 - WFG_STOP_DELAY - gt14
- 102.0e-6 - POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY
- PRG_START_DELAY - POWER_DELAY - WFG_START_DELAY
- 4.0e-6 - pwcgcob - WFG_STOP_DELAY - POWER_DELAY
- 4.0e-6);
decpower(d_cgcob);
decshaped_pulse(shcgcoib,pwcgcob,zero,4.0e-6,0.0);
decphase(t4);
}
else if(fCT[A] == 'n' && fc180[A] == 'n')
{
delay(tau1);
delay(tau1);
}
else if(fCT[A] == 'n' && fc180[A] == 'y')
{
initval(1.0,v4);
decstepsize(sphase);
dcplrphase(v4);
decpower(d_creb);
decshaped_pulse(shcreb,pwcreb,zero,4.0e-6,0.0);
dcplrphase(zero);
}
decpower(pwClvl);
decrgpulse(pwC,t4,4.0e-6,0.0);
delay(zeta - POWER_DELAY - 4.0e-6
- pwD - PRG_STOP_DELAY - DELAY_BLANK
- gt13
- 102.0e-6 - POWER_DELAY - WFG_START_DELAY);
/* Turn off D decoupling */
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3blank();
dec3power(pwDlvl);
dec3rgpulse(pwD,three,4.0e-6,0.0);
/* Turn off D decoupling */
delay(2.0e-6);
zgradpulse(gzlvl13,gt13);
delay(100.0e-6);
if (cal_sphase[A] == 'y')
{
decpower(pwClvl);
decshaped_pulse("hard",2.0*pwC,zero,4.0e-6,4.0e-6);
}
else
{
initval(1.0,v5);
decstepsize(sphase);
dcplrphase(v5);
decpower(d_creb);
decshaped_pulse(shcreb,pwcreb,zero,4.0e-6,4.0e-6);
dcplrphase(zero);
}
delay(2.0e-6);
zgradpulse(gzlvl13,gt13);
delay(100.0e-6);
/* Turn on D decoupling using the third decoupler */
dec3phase(one);
dec3power(pwDlvl);
dec3rgpulse(pwD,one,4.0e-6,0.0);
dec3phase(zero);
dec3power(dpwr3);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
/* Turn on D decoupling */
delay(zeta - WFG_STOP_DELAY - gt13 - 102.0e-6
- POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY
- PRG_START_DELAY - DELAY_BLANK
- POWER_DELAY - 4.0e-6);
decpower(pwClvl);
decrgpulse(pwC,zero,4.0e-6,0.0);
/* Turn off D decoupling */
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3blank();
dec3phase(three);
dec3power(pwDlvl);
dec3rgpulse(pwD,three,4.0e-6,0.0);
/* Turn off D decoupling */
delay(0.2e-6);
zgradpulse(gzlvl9,gt9);
delay(200.0e-6);
if (shared_CT[A] == 'n')
{
dec2rgpulse(pwN,t5,2.0e-6,0.0);
decpower(d_ca180);
dec2phase(t6);
delay(bigTN - tau2 - POWER_DELAY);
dec2rgpulse(2*pwN,t6,0.0,0.0);
decshaped_pulse(shca180,pwca180,zero,0.0,0.0);
dec2phase(t7);
delay(bigTN - WFG_START_DELAY - pwca180 - WFG_STOP_DELAY
- gt1 - 2.0*GRADIENT_DELAY - 500.2e-6
- POWER_DELAY - 4.0e-6 - WFG_START_DELAY
- pwco180 - WFG_STOP_DELAY);
delay(0.2e-6);
zgradpulse(gzlvl1,gt1);
delay(500.0e-6);
decpower(d_co180);
decshaped_pulse(shco180,pwco180,zero,4.0e-6,0.0);
delay(tau2);
sim3pulse(pw,0.0,pwN,zero,zero,t7,0.0,0.0);
}
else if (shared_CT[A] == 'y')
{
dec2rgpulse(pwN,t5,2.0e-6,0.0);
decpower(d_co180);
dec2phase(t6);
if (bigTN - tau2 >= 0.2e-6)
{
delay(tau2);
decshaped_pulse(shco180,pwco180,zero,4.0e-6,0.0);
decpower(d_ca180);
delay(0.2e-6);
zgradpulse(gzlvl1,gt1);
delay(500.0e-6);
delay(bigTN - 4.0e-6 - WFG_START_DELAY - pwco180 - WFG_STOP_DELAY
- POWER_DELAY - gt1 - 500.2e-6 - 2.0*GRADIENT_DELAY
- WFG_START_DELAY - pwca180 - WFG_STOP_DELAY);
decshaped_pulse(shca180,pwca180,zero,0.0,0.0);
dec2rgpulse(2*pwN,t6,0.0,0.0);
delay(bigTN - tau2);
}
else
{
delay(tau2);
decshaped_pulse(shco180,pwco180,zero,4.0e-6,0.0);
delay(0.2e-6);
zgradpulse(gzlvl1,gt1);
delay(500.0e-6);
decpower(d_ca180);
delay(bigTN - 4.0e-6 - WFG_START_DELAY - pwco180
- WFG_STOP_DELAY - gt1 - 500.2e-6 - 2.0*GRADIENT_DELAY
- POWER_DELAY - WFG_START_DELAY - pwca180 - WFG_STOP_DELAY);
decshaped_pulse(shca180,pwca180,zero,0.0,0.0);
delay(tau2 - bigTN);
dec2rgpulse(2.0*pwN,t6,0.0,0.0);
}
sim3pulse(pw,0.0,pwN,zero,zero,t7,0.0,0.0);
}
/* end of shared_CT */
if (nietl_flg[A] == 'n')
{
decpower(pwClvl);
decrgpulse(pwC,zero,4.0e-6,0.0);
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(2.0e-6);
dec2phase(zero);
delay(tauf - POWER_DELAY - 4.0e-6
- pwC - gt6 - 2.2e-6);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
txphase(one);
dec2phase(one);
delay(tauf - gt6 - 200.2e-6);
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(200.0e-6);
sim3pulse(pw,0.0,pwN,one,zero,one,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
txphase(zero);
dec2phase(zero);
delay(tauf - gt7 - 2.2e-6);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
delay(tauf - gt7 - 200.2e-6);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(200.0e-6);
sim3pulse(pw,0.0e-6,pwN,zero,zero,zero,0.0,0.0);
}
else
{ /* nietl_flg == y */
/* shaped pulse */
obspower(tpwrsl1);
shaped_pulse(shp_sl,pw_sl1,zero,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
decpower(pwClvl);
decrgpulse(pwC,zero,4.0e-6,0.0);
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(2.0e-6);
dec2phase(zero);
delay(tauf
- POWER_DELAY - 2.0e-6 - WFG_START_DELAY
- pw_sl1 - WFG_STOP_DELAY - 2.0e-6 - POWER_DELAY
- POWER_DELAY - 4.0e-6
- pwC - gt6 - 2.2e-6);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
txphase(one);
dec2phase(zero);
delay(tauf - gt6 - 200.2e-6);
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(200.0e-6);
sim3pulse(pw,0.0,pwN,one,zero,zero,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
txphase(zero);
dec2phase(zero);
delay(tauf - gt7 - 2.2e-6);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
txphase(one);
dec2phase(one);
delay(tauf - gt7 - 200.2e-6);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(200.0e-6);
sim3pulse(pw,0.0e-6,pwN,one,zero,one,0.0,0.0);
txphase(zero);
} /* end of nietl_flg == y */
delay(gt2 +gstab -0.5*(pwN-pw) -2.0*pw/PI);
rgpulse(2*pw,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(icosel*gzlvl2,gt2);
decpower(dpwr); /* NO 13C decoupling */
dec2power(dpwr2); /* NO 15N decoupling */
delay(gstab -2.0e-6 -2.0*GRADIENT_DELAY -2.0*POWER_DELAY);
lk_sample();
/* BEGIN ACQUISITION */
status(C);
setreceiver(t8);
}
void pulsesequence()
{
double gzlvlE = getval("gzlvlE"),
gtE = getval("gtE"),
EDratio = getval("EDratio"),
gstab = getval("gstab"),
mult = getval("mult"),
pwx180 = getval("pwx180"),
pwxlvl180 = getval("pwxlvl180"),
pwx180r = getval("pwx180r"),
pwxlvl180r = getval("pwxlvl180r"),
hsglvl = getval("hsglvl"),
hsgt = getval("hsgt"),
tauA=getval("tauA"), //compensation for tauB and tauD
tauB=getval("tauB"), //effect of rof2
tauD=getval("tauD"), //effect of alfa
tBal=getval("tBal"), //supports inova console if ~1/(fb*1.3)
pwr_XBIP = getval("pwr_XBIP"),
pw_XBIP = getval("pw_XBIP"),
pwr_HBIP = getval("pwr_HBIP"),
pw_HBIP = getval("pw_HBIP"),
gzlvlcr = getval("gzlvlcr"),
gtcr = getval("gtcr"), //crusher gradient in BIRD
npoints = getval("npoints"), // npoints should be an integer multiple of np
tau, evolcorr, taug,cycles;
int prgcycle = (int)(getval("prgcycle")+0.5),
phase1 = (int)(getval("phase")+0.5),
icosel, ZZgsign;
char pwx180ad[MAXSTR], pwx180adR[MAXSTR], pwx180ref[MAXSTR],
shp_XBIP[MAXSTR], shp_HBIP[MAXSTR], BIRD[MAXSTR], BIRDmode[MAXSTR];
//synchronize gradients to srate for probetype='nano'
// Preserve gradient "area"
gtE = syncGradTime("gtE","gzlvlE",0.5);
gzlvlE = syncGradLvl("gtE","gzlvlE",0.5);
getstr("pwx180ad", pwx180ad);
getstr("pwx180adR", pwx180adR);
getstr("pwx180ref", pwx180ref);
getstr("shp_XBIP",shp_XBIP);
getstr("shp_HBIP",shp_HBIP);
getstr("BIRD",BIRD);
getstr("BIRDmode",BIRDmode);
tau = 1 / (4*(getval("j1xh")));
evolcorr = (4*pwx/PI)+2*pw+8.0e-6;
cycles=np/npoints;
cycles = (double)((int)((cycles)));
initval(cycles,v20);
if (mult > 0.5)
taug = 2*tau + getval("tauC");
else
taug = gtE + gstab + 2 * GRADIENT_DELAY;
ZZgsign=-1;
if (mult == 2) ZZgsign=1;
icosel = 1;
assign(ct,v17);
assign(zero,v18);
assign(zero,v19);
if (getflag("prgflg") && (satmode[0] == 'y') && (prgcycle > 1.5))
{
hlv(ct,v17);
mod2(ct,v18); dbl(v18,v18);
if (prgcycle > 2.5)
{
hlv(v17,v17);
hlv(ct,v19); mod2(v19,v19); dbl(v19,v19);
}
}
if (BIRD[0]=='n') //gHSQC phases
{
settable(t1,4,ph1);
settable(t2,2,ph2);
settable(t3,8,ph3);
settable(t4,16,ph4);
settable(t5,16,ph5);
}
else //rtgHSQC-BIRD phases
{
settable(t1,8,ph11);
settable(t2,2,ph12);
settable(t3,16,ph13);
settable(t4,32,ph14);
settable(t5,32,ph15);
settable(t7,4,ph17);
settable(t8,4,ph18);
settable(t9,4,ph19);
getelem(t7, v17, v7);
getelem(t8, v17, v8);
getelem(t9, v17, v9);
}
getelem(t1, v17, v1);
getelem(t3, v17, v3);
getelem(t4, v17, v4);
getelem(t2, v17, v2);
getelem(t5, v17, oph);
assign(zero,v6);
add(oph,v18,oph);
add(oph,v19,oph);
if ((phase1 == 2) || (phase1 == 5))
icosel = -1;
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v14);
add(v2, v14, v2);
add(oph, v14, oph);
status(A);
obspower(tpwr); decpower(pwxlvl);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
if (satmode[0] == 'y')
{
if ((d1-satdly) > 0.02)
delay(d1-satdly);
else
delay(0.02);
if (getflag("slpsat"))
{
shaped_satpulse("relaxD",satdly,zero);
if (getflag("prgflg"))
shaped_purge(v6,zero,v18,v19);
}
else
{
satpulse(satdly,zero,rof1,rof1);
if (getflag("prgflg"))
purge(v6,zero,v18,v19);
}
}
else
delay(d1);
if (getflag("wet"))
wet4(zero,one);
status(B);
/****** null flag starts here *****/
if (getflag("nullflg"))
{
rgpulse(0.5 * pw, zero, rof1, rof1);
delay(2 * tau);
decpower(pwxlvl180);
decshaped_pulse(pwx180ad, pwx180, zero, rof1, rof1);
rgpulse(2.0 * pw, zero, rof1, rof1);
delay(2 * tau + 2 * POWER_DELAY);
decshaped_pulse(pwx180adR, pwx180, zero, rof1, rof1);
decpower(pwxlvl);
rgpulse(1.5 * pw, two, rof1, rof1);
zgradpulse(hsglvl, hsgt);
delay(1e-3);
}
/*********gHSQC or gHSQC part of pure shift starts here *****/
if (getflag("cpmgflg"))
{
rgpulse(pw, v6, rof1, 0.0);
cpmg(v6, v15);
}
else
rgpulse(pw, v6, rof1, rof1);
delay(tau);
decpower(pwxlvl180);
decshaped_pulse(pwx180ad, pwx180, zero, rof1, rof1);
rgpulse(2.0 * pw, zero, rof1, rof1);
delay(tau + 2 * POWER_DELAY);
decshaped_pulse(pwx180adR, pwx180, zero, rof1, rof1);
decpower(pwxlvl);
rgpulse(pw, v1, rof1, rof1);
zgradpulse(hsglvl, 2 * hsgt);
delay(1e-3);
decrgpulse(pwx, v2, rof1, 2.0e-6);
delay(d2 / 2);
rgpulse(2 * pw, zero, 2.0e-6, 2.0e-6);
delay(d2 / 2);
delay(taug - POWER_DELAY);
if (mult > 0.5)
{
decpower(pwxlvl180r);
decshaped_pulse(pwx180ref, pwx180r, zero, rof1, rof1);
rgpulse(mult * pw, zero, rof1, rof1);
delay(taug - mult * pw - 2 * rof1 + POWER_DELAY - gtE - gstab - 2 * GRADIENT_DELAY+evolcorr);
zgradpulse(gzlvlE, gtE);
delay(gstab);
decshaped_pulse(pwx180ref, pwx180r, zero, rof1, rof1);
}
else
{
decpower(pwxlvl180);
decshaped_pulse(pwx180ad, pwx180, zero, rof1, rof1);
delay(taug + POWER_DELAY - gtE - gstab - 2 * GRADIENT_DELAY+evolcorr);
zgradpulse(gzlvlE, gtE);
delay(gstab);
decshaped_pulse(pwx180ad, pwx180, zero, rof1, rof1);
}
decpower(pwxlvl);
decrgpulse(pwx, v4, 2.0e-6, rof1);
zgradpulse(ZZgsign*0.6 * hsglvl, 1.2 * hsgt);
delay(1e-3);
rgpulse(pw, v3, rof1, rof1);
decpower(pwxlvl180);
decshaped_pulse(pwx180adR, pwx180, zero, rof1, rof1);
decpower(dpwr);
delay(tau - (2 * pw / PI) - 2*rof1);
rgpulse(2 * pw, zero, rof1, rof2);
decpower(pwxlvl180);
decshaped_pulse(pwx180ad, pwx180, zero, rof1, rof1);
decpower(dpwr);
zgradpulse(icosel * 2.0*gzlvlE/EDratio, gtE/2.0);
delay(tau - gtE/2.0 - 2 * GRADIENT_DELAY);
/********gHSQC part stops and BIRD Acquisition starts here*************/
// delay(tBal);
//filter delay (Hoult) for inova; adjust tBal manually for the same effect
//delay(1.0/(getval("fb")*1.3))
if (BIRD[0]=='y')
{
#ifdef NVPSG
setacqmode(WACQ|NZ); //use this line only for vnmrs console; comment this out in inova
#endif
obsblank();
// delay(rof2);
startacq(alfa);
}
/*----------------------------------------------------------------------------
Observe the 1st half chunk
-----------------------------------------------------------------------------*/
if (BIRD[0]=='y')
{
status(C);
acquire(npoints/2.0,1.0/sw);
rcvroff();
status(B);
obspower(tpwr);
/*------------------------------------------------------------------------
Using hard 13C inversion pulse in BIRD
--------------------------------------------------------------------------*/
if (BIRDmode[0]== 'h')
{
rgpulse(pw,v7,rof1,rof1);
decpower(pwxlvl);
delay(tau);
zgradpulse(gzlvlcr,gtcr);
delay(tau-gtcr);
simpulse(2.0*pw,2.0*pwx,v8,v8,rof1,rof1);
decpower(dpwr);
delay(tau);
zgradpulse(gzlvlcr,gtcr);
delay(tau-gtcr);
rgpulse(pw,v9,rof1,rof1);
}
/*---------------------------------------------------------------------------
Using BIP 13C inversion pulse in BIRD
----------------------------------------------------------------------------*/
if (BIRDmode[0]== 'b')
{
rgpulse(pw,v7,rof1,rof1);
if (pwr_XBIP!=pwxlvl) decpower(pwr_XBIP); else decpower(pwxlvl);
if (pwr_HBIP!=tpwr) obspower(pwr_HBIP);
delay(tau);
zgradpulse(gzlvlcr,gtcr);
delay(tau-gtcr);
simshaped_pulse(shp_HBIP,shp_XBIP,2.0*pw,pw_XBIP,v8,v8,rof1,rof1);
if (pwr_HBIP!=tpwr) obspower(tpwr);
decpower(dpwr);
delay(tau);
zgradpulse(gzlvlcr,gtcr);
delay(tau-gtcr);
rgpulse(pw,v9,rof1,rof1);
}
delay(tauA);
rgpulse(pw*2.0,v7,rof1,rof1); // hard 180 degree refocusing pulse
obsblank();
delay(tauB);
rcvron(); //this includes rof3
delay(tauD);
decr(v20);
/*------------------------------------------------------------------------------
Loops for more chunks
------------------------------------------------------------------------------*/
starthardloop(v20);
status(C);
acquire(npoints,1.0/sw);
rcvroff();
status(B);
obspower(tpwr);
/*------------------------------------------------------------------------
Using hard 13C inversion pulse in BIRD
--------------------------------------------------------------------------*/
if (BIRDmode[0]== 'h')
{
rgpulse(pw,v7,rof1,rof1);
decpower(pwxlvl);
delay(tau);
zgradpulse(gzlvlcr,gtcr);
delay(tau-gtcr);
simpulse(2.0*pw,2.0*pwx,v8,v8,rof1,rof1);
decpower(dpwr);
delay(tau);
zgradpulse(gzlvlcr,gtcr);
delay(tau-gtcr);
rgpulse(pw,v9,rof1,rof1);
}
/*---------------------------------------------------------------------------
Using BIP 13C inversion pulse in BIRD
----------------------------------------------------------------------------*/
// if (BIRDmode[0]== 'b')
if (BIRDmode[0]== 'b')
{
rgpulse(pw,v7,rof1,rof1);
if (pwr_XBIP!=pwxlvl) decpower(pwr_XBIP); else decpower(pwxlvl);
delay(tau);
zgradpulse(gzlvlcr,gtcr);
delay(tau-gtcr);
simshaped_pulse(shp_HBIP,shp_XBIP,2*pw,pw_XBIP,v8,v8,rof1,rof1);
decpower(dpwr);
delay(tau);
zgradpulse(gzlvlcr,gtcr);
delay(tau-gtcr);
rgpulse(pw,v9,rof1,rof1);
}
delay(tauA);
rgpulse(pw*2.0,v7,rof1,rof1); // hard 180 degree refocusing pulse
obsblank();
delay(tauB);
rcvron(); //this includes rof3
delay(tauD);
endhardloop();
/*----------------------------------------------------------------
Acquisition of last half chunk
----------------------------------------------------------------*/
status(C);
acquire(npoints/2.0,1.0/sw);
rcvroff();
endacq();
incr(v20);
}
/****** BIRD ends here for all *****/
/***************** ACQ for conventional gHSQC ******************************/
else
status(C);
}
pulsesequence()
{
/* DECLARE VARIABLES */
char fscuba[MAXSTR],f1180[MAXSTR],f2180[MAXSTR],fsat[MAXSTR],
shape[MAXSTR],sh_ad[MAXSTR],f3180[MAXSTR],
N_flg[MAXSTR],diag_supp[MAXSTR];
int phase,
phase2,
phase3,
t1_counter,
t2_counter,
t3_counter, icosel;
double hscuba, /* length of 1/2 scuba delay */
pwx2, /* PW90 for X-nuc */
tsatpwr, /* low power level for presat*/
dhpwr2, /* power level for X hard pulses */
jxh, /* coupling for XH */
tauxh, /* delay = 1/(2jxh) */
tau1, /* t1/2 H */
tau2, /* t2/2 N */
tau3, /* t3/2 N */
sw1, /* spectral width in 1H dimension */
sw2, /* spectral width in 15N dimension */
sw3, /* spectral width in 15N dimension */
MIX, /* Total Mixing time for noesy portion */
pw_sl, /* selective 2ms pulse on water */
tpwrsl, /* power level for pw_sl */
ppm,nst,pws,bw,ofs, /* used by Pbox */
pwN,pwNlvl,compH,compC,pwC,pwClvl,
d_ad, /* C high power for adiabatic pulses */
pwc_ad, /* C 90 pulse width */
zeta, /* Bax-Logan trick */
zeta1, /* Bax-Logan trick */
BigT,
BigT1,
gzlvl0,
gzlvl1,
gzlvl2,
gzlvl3,
gzlvl4,
gzlvl5,
gzlvl6,
gzlvl7,
gzlvl8,
gzlvl9,
gzlvl10,
gzlvl11,
gzlvl12,
gstab,
gt1,
gt2,
gt3,
gt4,
gt5,
gt6,
gt7,
gt8,
gt9,
gt10,
gt11,
gt12;
/* LOAD VARIABLES */
pwNlvl=getval("pwNlvl");
pwN=getval("pwN");
compC=getval("compC");
pwC=getval("pwC");
pwClvl=getval("pwClvl");
compH=getval("compH");
jxh = getval("jxh");
dhpwr2 = getval("dhpwr2");
pwx2 = getval("pwx2");
tsatpwr = getval("tsatpwr");
hscuba = getval("hscuba");
phase = (int) (getval("phase") + 0.5);
phase2 = (int) (getval("phase2") + 0.5);
phase3 = (int) (getval("phase3") + 0.5);
sw1 = getval("sw1");
sw2 = getval("sw2");
sw3 = getval("sw3");
MIX = getval("MIX");
pw_sl = getval("pw_sl");
tpwrsl = getval("tpwrsl");
pwc_ad = getval("pwc_ad");
d_ad = getval("d_ad");
ni = getval("ni");
BigT = getval("BigT");
BigT1 = getval("BigT1");
gstab = getval("gstab");
gzlvl0 = getval("gzlvl0");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl3 = getval("gzlvl3");
gzlvl4 = getval("gzlvl4");
gzlvl5 = getval("gzlvl5");
gzlvl6 = getval("gzlvl6");
gzlvl7 = getval("gzlvl7");
gzlvl8 = getval("gzlvl8");
gzlvl9 = getval("gzlvl9");
gzlvl10 = getval("gzlvl10");
gzlvl11 = getval("gzlvl11");
gzlvl12 = getval("gzlvl12");
gt1 = getval("gt1");
gt2 = getval("gt2");
gt3 = getval("gt3");
gt4 = getval("gt4");
gt5 = getval("gt5");
gt6 = getval("gt6");
gt7 = getval("gt7");
gt8 = getval("gt8");
gt9 = getval("gt9");
gt10 = getval("gt10");
gt11 = getval("gt11");
gt12 = getval("gt12");
getstr("fscuba",fscuba);
getstr("fsat",fsat);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("f3180",f3180);
getstr("N_flg",N_flg);
getstr("diag_supp",diag_supp);
getstr("sh_ad",sh_ad);
getstr("shape",shape);
if(d_ad > 62) {
printf("chirp power is too high \n");
psg_abort(1);
}
if(pwc_ad > 1.2e-3) {
printf("adiabatic pulse is too long; set < 0.5 ms\n");
psg_abort(1);
}
setautocal(); /* activate auto-calibration flags */
if (autocal[0] != 'n')
/* if autocal = 'y'(yes), 'q'(quiet), r(read), or 's'(semi) */
{
strcpy(shape,"H2Osel");
strcpy(sh_ad,"C13adiab");
if(FIRST_FID) /* call Pbox */
{
ppm = getval("dfrq"); ofs = 0.0; pws = 0.0005; /*0.5 ms pulse */
bw = 200.0*ppm; nst = 1000; /* nst - number of steps */
C13adiab = pbox_makeA("C13adiab", "sech", bw, pws, ofs, compC*pwC, pwClvl, nst);
H2Osel = pbox_Rsh("H2Osel", "sinc90", pw_sl, 0.0, compH*pw, tpwr);
ofs_check(H1ofs, C13ofs, N15ofs, H2ofs);
}
pw_sl = H2Osel.pw; tpwrsl = H2Osel.pwr-1.0; /* 1dB correction applied */
d_ad = C13adiab.pwr; pwc_ad = C13adiab.pw;
pwx2=pwN; dhpwr2=pwNlvl;
}
/* check validity of parameter range */
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y'))
{
printf("incorrect Dec1 decoupler flags! ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y'))
{
printf("incorrect Dec2 decoupler flags! ");
psg_abort(1);
}
if( tsatpwr > 8 )
{
printf("tsatpwr too large !!! ");
psg_abort(1);
}
if( dpwr > -16 )
{
printf("No C decoupling used in this expt. ");
psg_abort(1);
}
if( dpwr2 > -16 )
{
printf("No N decoupling used in this expt. ");
psg_abort(1);
}
if(gzlvl0 > 500) {
printf("gzlvl0_max is 500\n");
psg_abort(1);
}
if( gt1 > 3e-3 || gt2 > 3e-3 || gt3 > 3e-3 || gt4 > 3e-3
|| gt5 > 3e-3 || gt6 > 3e-3 || gt7 > 3e-3
|| gt8 > 3e-3 || gt9 > 3e-3 || gt10 > 3e-3 || gt11 > 3e-3
|| gt12 > 3e-3)
{
printf("gradients are on for too long !!! ");
psg_abort(1);
}
if(ix==1) {
if(f1180[A] != 'n' || f2180[A] !='y' || f3180[A] != 'y') {
printf("f1180 should be n, f2180 y, and f3180 should be y\n");
}
}
/* LOAD VARIABLES */
settable(t1, 4, phi1);
settable(t2, 1, phi2);
settable(t3, 1, phi3);
settable(t4, 1, phi4);
settable(t5, 1, phi5);
settable(t6, 1, phi6);
settable(t7, 1, phi7);
settable(t8, 1, phi8);
settable(t9, 2, phi9);
settable(t10, 4, rec);
/* INITIALIZE VARIABLES */
tauxh = 1/(4*jxh);
/* Phase incrementation for hypercomplex data */
if( phase == 2 ) {
tsadd(t2, 1, 4);
tsadd(t3, 1, 4);
}
if ( phase2 == 2 ) { /* Hypercomplex in t2 */
tsadd(t1, 1, 4);
}
if ( phase3 == 2 ) /* Hypercomplex in t3 */
{
tsadd(t6, 2, 4);
tsadd(t7, 2, 4);
tsadd(t8, 2, 4);
tsadd(t10, 2, 4);
icosel = -1;
}
else
icosel = 1;
/* calculate modifications to phases based on current t2/t3 values
to achieve States-TPPI acquisition */
if(ix==1)
d4_init = d4;
t3_counter = (int) ( (d4-d4_init)*sw3 + 0.5);
if(t3_counter %2) {
tsadd(t4,2,4);
tsadd(t5,2,4);
tsadd(t10,2,4);
}
if(ix==1)
d3_init = d3;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5);
if(t2_counter %2) {
tsadd(t1,2,4);
tsadd(t10,2,4);
}
if(ix==1)
d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5);
if(t1_counter %2) {
tsadd(t2,2,4);
tsadd(t3,2,4);
tsadd(t10,2,4);
}
/* set up so that get (-90,180) phase corrects in F1 if f1180 flag is y */
tau1 = d2;
if(f1180[A] == 'y') {
tau1 += 1/(2.0*sw1);
}
tau1 = tau1/2.0;
if(tau1 < 0.2e-6) tau1 = 0.2e-6;
/* set up so that get (-90,180) phase corrects in F2 if f2180 flag is y */
tau2 = d3;
if(f2180[A] == 'y')
tau2 += ( 1.0/(2.0*sw2) - (4.0/PI)*pwx2
- 2.0*(2.0*GRADIENT_DELAY + 50.0e-6 + 5.0e-6) );
if(f2180[A] == 'n')
tau2 = ( tau2 - (4.0/PI)*pwx2
- 2.0*(2.0*GRADIENT_DELAY + 50.0e-6 + 5.0e-6) );
tau2 = tau2/2.0;
if(ix==1)
if((tau2 + 5.0e-6 - 0.5*(WFG_START_DELAY + 4.0*pw + WFG_STOP_DELAY)
< 5.0e-6) && N_flg[A] == 'n')
printf("tau2 is negative; set f1180 to y\n");
/* set up so that get (-90,180) phase corrects in F3 if f3180 flag is y */
tau3 = d4;
if(f3180[A] == 'y') tau3 += ( 1.0/(2.0*sw3) );
tau3 = tau3/2.0;
if( tau3 < 0.2e-6) tau3 = 2.0e-7;
/* Now include Bax/Logan trick */
if(ni != 1) {
if(diag_supp[A] == 'n')
zeta = (tauxh - gt5 - 102.0e-6 + 2.0*pwx2 - 2.0e-6);
else
zeta = (1.0/(8.0*93.39) - gt5 - 102.0e-6 + 2.0*pwx2 - 2.0e-6);
zeta = zeta / ( (double) (ni-1) );
if(zeta < 0.0) {
printf("problem with zeta\n");
psg_abort(1);
}
}
else
zeta = 0.0;
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2 - d2_init)*sw1 + 0.5 );
zeta1 = zeta*( (double)t1_counter );
tau1 = tau1 - zeta1;
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tsatpwr); /* Set power for presaturation */
decpower(d_ad); /* Set decoupler1 power to d_ad */
dec2power(dhpwr2); /* Set decoupler2 power to dhpwr2 */
/* Presaturation Period */
if(fsat[0] == 'y')
{
txphase(zero);
rgpulse(d1,zero,2.0e-6,2.0e-6); /* presat */
obspower(tpwr); /* Set power for hard pulses */
if (fscuba[0] == 'y') /* Scuba pulse sequence */
{
hsdelay(hscuba);
rgpulse(pw,zero,1.0e-6,0.0); /* 90x180y90x */
rgpulse(2*pw,one,1.0e-6,0.0);
rgpulse(pw,zero,1.0e-6,0.0);
txphase(zero);
delay(hscuba);
}
}
else {
obspower(tpwr); /* Set power for hard pulses */
delay(d1);
}
status(B);
obsoffset(tof);
rcvroff();
delay(20.0e-6);
/* eliminate all magnetization originating from 15N */
dec2rgpulse(pwx2,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl1,gt1);
delay(gstab);
rgpulse(pw,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl2,gt2);
delay(2.0e-6);
delay(tauxh - gt2 - 4.0e-6); /* delay=1/4J(XH) */
sim3pulse(2*pw,0.0e-6,2*pwx2,zero,zero,zero,0.0,0.0);
delay(tauxh - gt2 - 202.0e-6); /* delay=1/4J(XH) */
txphase(one); dec2phase(t1);
delay(2.0e-6);
zgradpulse(gzlvl2,gt2);
delay(gstab);
rgpulse(pw,one,0.0,0.0);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,two,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
delay(2.0e-6);
zgradpulse(gzlvl3,gt3);
delay(gstab);
txphase(zero);
dec2rgpulse(pwx2,t1,0.0,0.0);
dec2phase(zero);
if(N_flg[A] == 'n')
{
if(tau2 + 5.0e-6 - 0.5*(WFG2_START_DELAY
+ pwc_ad + WFG2_STOP_DELAY) < 0.2e-6)
{
rgradient('z',gzlvl0); /* use rgradient since shaping takes more time */
delay(tau2 + 5.0e-6 - 0.5*(WFG_START_DELAY + 4.0*pw + WFG_STOP_DELAY));
rgradient('z',0.0);
delay(50.0e-6);
shaped_pulse("composite",4.0*pw,zero,0.0,0.0); /* 90x-180y-90x */
rgradient('z',-1.0*gzlvl0);
delay(tau2 + 5.0e-6 - 0.5*(WFG_START_DELAY + 4.0*pw + WFG_STOP_DELAY));
rgradient('z',0.0);
delay(50.0e-6);
}
else
{
rgradient('z',gzlvl0); /* use rgradient since shaping takes more time */
delay(tau2 + 5.0e-6 - 0.5*(WFG2_START_DELAY
+ pwc_ad + WFG2_STOP_DELAY));
rgradient('z',0.0);
delay(50.0e-6);
simshaped_pulse("composite",sh_ad,4.0*pw,pwc_ad,zero,zero,0.0,0.0);
rgradient('z',-1.0*gzlvl0); /* use rgradient since shaping takes more time */
delay(tau2 + 5.0e-6 - 0.5*(WFG2_START_DELAY
+ pwc_ad + WFG2_STOP_DELAY));
rgradient('z',0.0);
delay(50.0e-6);
}
} /* N_flg[A] == y */
else
sim3pulse(2.0*pw,0.0,2.0*pwx2,zero,zero,zero,4.0e-6,4.0e-6);
dec2rgpulse(pwx2,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl4,gt4);
delay(gstab);
if(diag_supp[A] == 'n') {
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,t3,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
rgpulse(pw,t2,2.0e-6,0.0);
txphase(t9);
delay(tau1 + tauxh + zeta1 - gt5 - 102.0e-6);
delay(2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(100.0e-6);
dec2rgpulse(2.0*pwx2,zero,0.0,0.0);
delay(tau1);
rgpulse(2.0*pw,t9,0.0,0.0); txphase(one);
delay(tauxh - zeta1 - gt5 - 102.0e-6 + 2.0*pwx2);
delay(2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(100.0e-6);
rgpulse(pw,one,0.0,0.0);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,one,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
delay(MIX - gt6 - gstab -2.0e-6);
delay(2.0e-6);
zgradpulse(gzlvl6,gt6);
delay(gt6);
delay(gstab);
}
else {
initval(1.0,v4);
obsstepsize(45.0);
xmtrphase(v4);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,t3,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
/*
xmtrphase(zero);
delay(2.0e-6);
initval(1.0,v4);
obsstepsize(45.0);
xmtrphase(v4);
*/
rgpulse(pw,t2,2.0e-6,0.0);
xmtrphase(zero);
txphase(t9);
delay(tau1 + 1.0/(8.0*93.39) + zeta1 - gt5 - 102.0e-6 - SAPS_DELAY);
delay(2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(100.0e-6);
dec2rgpulse(2.0*pwx2,zero,0.0,0.0);
delay(tau1);
rgpulse(2.0*pw,t9,0.0,0.0); txphase(one);
delay(1.0/(8.0*93.39) - zeta1 - gt5 - 102.0e-6 + 2.0*pwx2);
delay(2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(100.0e-6);
rgpulse(pw,one,0.0,0.0);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,one,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
delay(2.0e-6);
zgradpulse(gzlvl6,gt6/2.0);
delay(gstab);
delay(MIX - 1.5*gt6 - 2.0*(gstab+2.0e-6) - 2.0*pwx2);
dec2rgpulse(2.0*pwx2,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl6,gt6);
delay(gstab);
}
rgpulse(pw,two,2.0e-6,0.0);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,zero,2.0e-6,0.0);
delay(2.0e-6); txphase(zero);
obspower(tpwr);
/* shaped pulse */
delay(2.0e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
delay(tauxh
- POWER_DELAY - 2.0e-6 - WFG_START_DELAY
- pw_sl - WFG_STOP_DELAY - 2.0e-6 - POWER_DELAY
- gt7 - 4.0e-6); /* delay=1/4J(XH) */
sim3pulse(2*pw,0.0e-6,2*pwx2,zero,zero,zero,0.0,0.0);
delay(tauxh
- gt7 - gstab -2.0e-6
- POWER_DELAY - 2.0e-6 - WFG_START_DELAY
- pw_sl - WFG_STOP_DELAY - 2.0e-6 - POWER_DELAY);
delay(2.0e-6);
zgradpulse(gzlvl7,gt7);
delay(gt7);
delay(gstab);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,one,2.0e-6,0.0);
obspower(tpwr);
delay(2.0e-6); txphase(zero);
/* shaped pulse */
rgpulse(pw,one,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl8,gt8);
delay(gstab);
txphase(t6);
if(phase3 == 1)
dec2rgpulse(pwx2,t4,4.0e-6,0.0);
if(phase3 == 2)
dec2rgpulse(pwx2,t5,4.0e-6,0.0);
decphase(zero);
if(tau3 - 0.5*(WFG_START_DELAY
+ pwc_ad + WFG_STOP_DELAY)
< 0.2e-6) {
delay(tau3);
delay(tau3);
}
else {
delay(tau3 - 0.5*(WFG_START_DELAY
+ pwc_ad + WFG_STOP_DELAY));
decshaped_pulse(sh_ad,pwc_ad,zero,0.0,0.0);
delay(tau3 - 0.5*(WFG_START_DELAY
+ pwc_ad + WFG_STOP_DELAY));
}
delay(2.0e-6);
zgradpulse(-1.0*gzlvl11,gt11);
delay(100.0e-6);
delay(BigT - 4.0/PI*pwx2 + pw - 2.0*GRADIENT_DELAY - gt11
- 102.0e-6);
dec2rgpulse(2.0*pwx2,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl11,gt11);
delay(100.0e-6);
delay(BigT - 2.0*GRADIENT_DELAY - gt11
- 102.0e-6);
rgpulse(pw,t6,0.0,0.0);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,t7,2.0e-6,0.0);
delay(2.0e-6); txphase(zero);
obspower(tpwr);
/* shaped pulse */
delay(2.0e-6);
zgradpulse(gzlvl9,gt9);
delay(100.0e-6);
delay(tauxh
- POWER_DELAY - 2.0e-6 - WFG_START_DELAY
- pw_sl - WFG_STOP_DELAY - 2.0e-6 - POWER_DELAY
- gt9 - 102.0e-6);
sim3pulse(2.0*pw,0.0,2.0*pwx2,zero,zero,zero,0.0,0.0);
dec2phase(t8);
delay(2.0e-6);
zgradpulse(gzlvl9,gt9);
delay(100.0e-6);
delay(tauxh
- gt9 - 102.0e-6
- POWER_DELAY - 2.0e-6 - WFG_START_DELAY
- pw_sl - WFG_STOP_DELAY - 2.0e-6 - POWER_DELAY);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,zero,2.0e-6,0.0);
obspower(tpwr);
delay(2.0e-6); txphase(zero);
/* shaped pulse */
sim3pulse(pw,0.0,pwx2,zero,zero,t8,0.0,0.0);
dec2phase(zero);
delay(2.0e-6);
zgradpulse(gzlvl10,gt10);
delay(2.0e-6);
delay(tauxh - gt10 - 4.0e-6);
sim3pulse(2*pw,0.0e-6,2*pwx2,zero,zero,zero,2.0e-6,2.0e-6);
delay(2.0e-6);
zgradpulse(gzlvl10,gt10);
delay(100.0e-6);
delay(tauxh
- gt10 - 102.0e-6
+ 2.0/PI*pw - pwx2 + 0.5*(pwx2-pw));
dec2rgpulse(pwx2,zero,0.0,0.0);
dec2power(dpwr2); /* Very Important */
decpower(dpwr);
delay(BigT1 - 2.0*POWER_DELAY);
rgpulse(2.0*pw,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(-1.0*icosel*gzlvl12,gt12);
delay(50.0e-6);
delay(BigT1 - 2.0*GRADIENT_DELAY - 52.0e-6 - gt12);
/* acquire data */
status(C);
setreceiver(t10);
}
pulsesequence()
{
double mix,SSpwr,pwSS,hsdly,pwa,pwd,gt1,gzlvl1,gzlvl2;
char ptable[MAXSTR],SSshape[MAXSTR];
getstr("ptable",ptable); getstr("SSshape",SSshape);
mix=getval("mix"); hsdly=getval("hsdly");
loadtable(ptable);
pwa=getval("pwa"); pwd=getval("pwd");
gt1=getval("gt1"); gzlvl1=getval("gzlvl1"); gzlvl2=getval("gzlvl2");
SSpwr=getval("SSpwr"); pwSS=getval("pwSS");
initval(7.0,v2); /* set up 45 degree phase shift for first pulse */
obsstepsize(45.0);
getelem(t1,ct,v1); /* 1st pulse phase */
getelem(t3,ct,v3); /* read pulse phase */
getelem(t4,ct,v4); /* receiver phase */
if (phase1 > 1.5) incr(v1); /* hypercomplex phase shift */
assign(v4,oph);
/*HYPERCOMPLEX MODE USES REDFIELD TRICK TO MOVE AXIAL PEAKS TO EDGE */
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v6);
if ((phase1==1)||(phase1==2)) {add(v1,v6,v1); add(v4,v6,oph);}
assign(v3,v7); /* for steering pulses */
add(v3,one,v8);
if (pwa<0.0) { pwa = -pwa; add(v7,two,v7); }
if (pwd<0.0) { pwd = -pwd; add(v8,two,v8); }
/* CHECK VALIDITY OF PARAMETER RANGE */
if (dm[A] == 'y' || dm[B] == 'y')
{
printf(" dm must be 'nny' or 'nnn' ");
psg_abort(1);
}
if ((dm[C] == 'y' || dm2[C] == 'y') && at > 0.085)
{
printf(" check at time! Don't fry probe \n");
psg_abort(1);
}
if (dm2[A] == 'y' || dm2[B] == 'y')
{
printf(" dm2 must be 'nny' or 'nnn' ");
psg_abort(1);
}
if (dpwr > 50)
{
printf(" dpwr must be less than 49 \n");
psg_abort(1);
}
if (dpwr2 > 50)
{
printf(" dpwr2 must be less than 46 \n");
psg_abort(1);
}
/* BEGIN PULSE SEQUENCE */
status(A);
xmtrphase(v2);
txphase(v1);
obspower(tpwr);
hsdelay(d1);
status(B);
rgpulse(pw, v1, rof1, 1.0e-6);
xmtrphase(zero);
txphase(t2);
if (d2 > 0.001)
{
zgradpulse(gzlvl2,0.4*d2-SAPS_DELAY/2.0-2.0*GRADIENT_DELAY-(2.0*pw/PI));
delay(0.1*d2-rof1);
zgradpulse(-gzlvl2,0.4*d2-SAPS_DELAY/2.0-2.0*GRADIENT_DELAY-(2.0*pw/PI));
delay(0.1*d2-rof1);
}
else delay(d2);
rgpulse(pw, t2, rof1, 1.0e-6);
status(C);
obspower(SSpwr);
delay(hsdly);
zgradpulse(gzlvl1,gt1);
hsdelay(mix-hsdly-gt1);
shaped_pulse(SSshape,pwSS,t3,rof1,0.0);
obspower(SSpwr-12.0);
rgpulse(pwa,v7,2.0e-6,0.0); /* steering pulses */
rgpulse(pwd,v8,0.0e-6,0.0);
delay(rof2);
status(D);
}
void pulsesequence()
{
double ss,
arraydim,
p1lvl,
trim,
mix,
window,
cycles,
phase;
int iphase;
char sspul[MAXSTR];
/* LOAD AND INITIALIZE VARIABLES */
ni = getval("ni");
arraydim = getval("arraydim");
mix = getval("mix");
trim = getval("trim");
phase = getval("phase");
iphase = (int) (phase + 0.5);
p1lvl = getval("p1lvl");
ss = getval("ss");
window=getval("window");
getstr("sspul", sspul);
if (iphase == 3)
{
initval((double)((int)((ix-1)/(arraydim/ni)+1.0e-6)), v14);
}
else
{
assign(zero, v14);
}
/* CHECK CONDITIONS */
if ((iphase != 3) && (arrayelements > 2))
{
fprintf(stdout, "PHASE=3 is required if MIX is arrayed!\n");
psg_abort(1);
}
if (satdly > 9.999)
{
fprintf(stdout, "Presaturation period is too long.\n");
psg_abort(1);
}
if (!newtransamp)
{
fprintf(stdout, "TOCSY requires linear amplifiers on transmitter.\n");
fprintf(stdout, "Use DECTOCSY with the appropriate re-cabling,\n");
psg_abort(1);
}
if ((p1 == 0.0) && (ix == 1))
fprintf(stdout, "Warning: P1 has a zero value.\n");
if ((rof1 < 9.9e-6) && (ix == 1))
fprintf(stdout,"Warning: ROF1 is less than 10 us\n");
if (satpwr > 40)
{
printf("satpwr too large - acquisition aborted./n");
psg_abort(1);
}
/* DETERMINE STEADY-STATE MODE */
if (ss < 0)
{
ss = (-1) * ss;
}
else
{
if ((ss > 0) && (ix == 1))
{
ss = ss;
}
else
{
ss = 0;
}
}
initval(ss, ssctr);
initval(ss, ssval);
/* STEADY-STATE PHASECYCLING */
/* This section determines if the phase calculations trigger off of (SS - SSCTR)
or off of CT */
ifzero(ssctr);
hlv(ct, v13);
mod2(ct, v1);
hlv(ct, v2);
elsenz(ssctr);
sub(ssval, ssctr, v12); /* v12 = 0,...,ss-1 */
hlv(v12, v13);
mod2(v12, v1);
hlv(v12, v2);
endif(ssctr);
/* CALCULATE PHASES */
/* A 2-step cycle is performed on the first pulse (90 degrees) to suppress
axial peaks in the first instance. Second, the 2-step F2 quadrature image
suppression subcycle is added to all pulse phases and receiver phase.
Finally, a 2-step cycle is performed on the spin-lock pulses. */
mod2(v13, v13);
dbl(v1, v1);
incr(v1);
hlv(v2, v2);
mod2(v2, v2);
dbl(v2, v2);
incr(v2);
add(v13, v2, v2);
sub(v2, one, v3);
add(two, v2, v4);
add(two, v3, v5);
add(v1, v13, v1);
assign(v1, oph);
if (iphase == 2)
incr(v1);
if (iphase == 3)
add(v1, v14, v1);
/*HYPERCOMPLEX MODE USES REDFIELD TRICK TO MOVE AXIAL PEAKS TO EDGE*/
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v6);
if ((iphase==1)||(iphase==2)) {add(v1,v6,v1); add(oph,v6,oph);}
/* CALCULATE AND INITIALIZE LOOP COUNTER */
if (pw > 0.0)
{
cycles = (mix - trim) / (64.66*pw+32*window);
cycles = 2.0*(double) (int) (cycles/2.0);
}
else
{
cycles = 0.0;
}
initval(cycles, v9); /* V9 is the MIX loop count */
/* BEGIN ACTUAL PULSE SEQUENCE CODE */
status(A);
rlpower(p1lvl, TODEV);
if (sspul[0] == 'y')
{
rgpulse(1000*1e-6, zero, rof1, 0.0e-6);
rgpulse(1000*1e-6, one, 0.0e-6, rof1);
}
hsdelay(d1);
if (getflag("wet")) wet4(zero,one);
rlpower(p1lvl, TODEV);
if (satmode[A] == 'y')
{ rlpower(satpwr,TODEV);
rgpulse(satdly,zero,rof1,rof2);
rlpower(p1lvl,TODEV);}
status(B);
rgpulse(p1, v1, rof1, 1.0e-6);
if (satmode[B] =='y')
{
if (d2 > 0.0)
{
rlpower(satpwr,TODEV);
rgpulse(d2 - (2*POWER_DELAY) - 1.0e-6 - (2*p1/3.1416),zero,0.0,0.0);
}
}
else
{
if (d2 > 0.0)
delay(d2 - POWER_DELAY - 1.0e-6 - (2*p1/3.1416));
}
rcvroff();
rlpower(tpwr,TODEV);
txphase(v13);
xmtron();
delay(trim);
if (cycles > 1.0)
{
starthardloop(v9);
mleva(window); mleva(window); mlevb(window); mlevb(window);
mlevb(window); mleva(window); mleva(window); mlevb(window);
mlevb(window); mlevb(window); mleva(window); mleva(window);
mleva(window); mlevb(window); mlevb(window); mleva(window);
rgpulse(.66*pw,v3,rof1,rof2);
endhardloop();
}
txphase(v13);
xmtroff();
/* detection */
delay(rof2);
rcvron();
status(C);
}