B-recs is a software able to reconstruct superresolution images at intermediate densities of active fluorophores. Active fluorophores must be sparse but their response on the camera can overlap. The theoretical limit for accurate reconstruction is one fluorophore per pixel on the camera, at higher densities, the accuracy quickly drops.

It is programmed in C, accompanied by a Image J / Fiji plugin.

B-recs uses cmake for the build process. You can refer to the cmake documentation for more details.

A few standard libraries are required:

• libbz2: each reconstructed image is stored as a raw image compressed with Bzip2.

• libtiff: input to B-recs are provided in the tiff format. Moreover, intermediate images can be produced in tiff format.

• fftw3: Fourier transforms are not used in the algorithms itself but is used in the case of strong autofluorescence to have a local estimate of the noise and background level.

In its present state, the program requires a modern cpu and compiler. More specifically, it uses SSE3 or AVX intrinsics. It means that the compiler should be able to understand them and the cpu support these instructions. I might implement a non-SIMD version at some point but I don't plan to do it yet.

mkdir build
cd build
cmake ..
make
make install

You should then be able to run brecs-reconssparse --version. For more details, please refer to the documentation available here.

B-recs is distributed under a BSD license (see LICENSE file).