int
main(int argc, char **argv) {
char *gkpName = 0L;
char *ovsName = 0L;
char *iniClrName = NULL;
char *maxClrName = NULL;
char *outClrName = NULL;
uint32 errorValue = AS_OVS_encodeEvalue(0.015);
uint32 minAlignLength = 40;
uint32 minReadLength = 64;
char *outputPrefix = NULL;
char logName[FILENAME_MAX] = {0};
char sumName[FILENAME_MAX] = {0};
FILE *logFile = 0L;
FILE *sumFile = 0L;
uint32 idMin = 1;
uint32 idMax = UINT32_MAX;
uint32 minEvidenceOverlap = 40;
uint32 minEvidenceCoverage = 1;
argc = AS_configure(argc, argv);
int arg=1;
int err=0;
while (arg < argc) {
if (strcmp(argv[arg], "-G") == 0) {
gkpName = argv[++arg];
} else if (strcmp(argv[arg], "-O") == 0) {
ovsName = argv[++arg];
} else if (strcmp(argv[arg], "-Ci") == 0) {
iniClrName = argv[++arg];
} else if (strcmp(argv[arg], "-Cm") == 0) {
maxClrName = argv[++arg];
} else if (strcmp(argv[arg], "-Co") == 0) {
outClrName = argv[++arg];
} else if (strcmp(argv[arg], "-e") == 0) {
double erate = atof(argv[++arg]);
errorValue = AS_OVS_encodeEvalue(erate);
} else if (strcmp(argv[arg], "-l") == 0) {
minAlignLength = atoi(argv[++arg]);
} else if (strcmp(argv[arg], "-minlength") == 0) {
minReadLength = atoi(argv[++arg]);
} else if (strcmp(argv[arg], "-ol") == 0) {
minEvidenceOverlap = atoi(argv[++arg]);
} else if (strcmp(argv[arg], "-oc") == 0) {
minEvidenceCoverage = atoi(argv[++arg]);
} else if (strcmp(argv[arg], "-o") == 0) {
outputPrefix = argv[++arg];
} else if (strcmp(argv[arg], "-t") == 0) {
AS_UTL_decodeRange(argv[++arg], idMin, idMax);
} else {
fprintf(stderr, "ERROR: unknown option '%s'\n", argv[arg]);
err++;
}
arg++;
}
if ((gkpName == NULL) ||
(ovsName == NULL) ||
(outputPrefix == NULL) ||
(err)) {
fprintf(stderr, "usage: %s -G gkpStore -O ovlStore -Co output.clearFile -o outputPrefix\n", argv[0]);
fprintf(stderr, "\n");
fprintf(stderr, " -G gkpStore path to read store\n");
fprintf(stderr, " -O ovlStore path to overlap store\n");
fprintf(stderr, "\n");
fprintf(stderr, " -o name output prefix, for logging\n");
fprintf(stderr, "\n");
fprintf(stderr, " -t bgn-end limit processing to only reads from bgn to end (inclusive)\n");
fprintf(stderr, "\n");
fprintf(stderr, " -Ci clearFile path to input clear ranges (NOT SUPPORTED)\n");
//fprintf(stderr, " -Cm clearFile path to maximal clear ranges\n");
fprintf(stderr, " -Co clearFile path to ouput clear ranges\n");
fprintf(stderr, "\n");
fprintf(stderr, " -e erate ignore overlaps with more than 'erate' percent error\n");
//fprintf(stderr, " -l length ignore overlaps shorter than 'l' aligned bases (NOT SUPPORTED)\n");
fprintf(stderr, "\n");
fprintf(stderr, " -ol l the minimum evidence overlap length\n");
fprintf(stderr, " -oc c the minimum evidence overlap coverage\n");
fprintf(stderr, " evidence overlaps must overlap by 'l' bases to be joined, and\n");
fprintf(stderr, " must be at least 'c' deep to be retained\n");
fprintf(stderr, "\n");
fprintf(stderr, " -minlength l reads trimmed below this many bases are deleted\n");
fprintf(stderr, "\n");
exit(1);
}
gkStore *gkp = gkStore::gkStore_open(gkpName);
ovStore *ovs = new ovStore(ovsName, gkp);
clearRangeFile *iniClr = (iniClrName == NULL) ? NULL : new clearRangeFile(iniClrName, gkp);
clearRangeFile *maxClr = (maxClrName == NULL) ? NULL : new clearRangeFile(maxClrName, gkp);
clearRangeFile *outClr = (outClrName == NULL) ? NULL : new clearRangeFile(outClrName, gkp);
if (outClr)
// If the outClr file exists, those clear ranges are loaded. We need to reset them
// back to 'untrimmed' for now.
outClr->reset(gkp);
if (iniClr && outClr)
// An iniClr file was supplied, so use those as the initial clear ranges.
outClr->copy(iniClr);
if (outputPrefix) {
sprintf(logName, "%s.log", outputPrefix);
sprintf(sumName, "%s.summary", outputPrefix);
errno = 0;
logFile = fopen(logName, "w");
if (errno)
fprintf(stderr, "Failed to open log file '%s' for writing: %s\n", logName, strerror(errno)), exit(1);
sumFile = fopen(sumName, "w");
if (errno)
fprintf(stderr, "Failed to open summary file '%s' for writing: %s\n", sumName, strerror(errno)), exit(1);
fprintf(logFile, "id\tinitL\tinitR\tfinalL\tfinalR\tmessage (DEL=deleted NOC=no change MOD=modified)\n");
fprintf(sumFile, "Overlap error rate <= %.4f fraction error\n", AS_OVS_decodeEvalue(errorValue));
fprintf(sumFile, "Overlap min overlap >= %u base%s (for 'largest covered')\n", minEvidenceOverlap, (minEvidenceOverlap == 1) ? "" : "s");
fprintf(sumFile, "Overlap min coverage >= %u read%s (for 'largest covered')\n", minEvidenceCoverage, (minEvidenceCoverage == 1) ? "" : "s");
}
uint32 ovlLen = 0;
uint32 ovlMax = 64 * 1024;
ovOverlap *ovl = ovOverlap::allocateOverlaps(gkp, ovlMax);
memset(ovl, 0, sizeof(ovOverlap) * ovlMax);
char logMsg[1024] = {0};
if (idMin < 1)
idMin = 1;
if (idMax > gkp->gkStore_getNumReads())
idMax = gkp->gkStore_getNumReads();
fprintf(stderr, "Processing from ID "F_U32" to "F_U32" out of "F_U32" reads.\n",
idMin,
idMax,
gkp->gkStore_getNumReads());
for (uint32 id=idMin; id<=idMax; id++) {
gkRead *read = gkp->gkStore_getRead(id);
gkLibrary *libr = gkp->gkStore_getLibrary(read->gkRead_libraryID());
logMsg[0] = 0;
// If the fragment is deleted, do nothing. If the fragment was deleted AFTER overlaps were
// generated, then the overlaps will be out of sync -- we'll get overlaps for these fragments
// we skip.
//
if ((iniClr) && (iniClr->isDeleted(id) == true))
continue;
// If it did not request trimming, do nothing. Similar to the above, we'll get overlaps to
// fragments we skip.
//
if ((libr->gkLibrary_finalTrim() == FINALTRIM_LARGEST_COVERED) &&
(libr->gkLibrary_finalTrim() == FINALTRIM_BEST_EDGE))
continue;
// Decide on the initial trimming. We copied any iniClr into outClr above, and if there wasn't
// an iniClr, then outClr is the full read.
uint32 ibgn = outClr->bgn(id);
uint32 iend = outClr->end(id);
// Set the, ahem, initial final trimming.
bool isGood = false;
uint32 fbgn = ibgn;
uint32 fend = iend;
// Load overlaps.
uint32 nLoaded = ovs->readOverlaps(id, ovl, ovlLen, ovlMax);
// Trim!
if (nLoaded == 0) {
// No overlaps, so mark it as junk.
isGood = false;
}
else if (libr->gkLibrary_finalTrim() == FINALTRIM_LARGEST_COVERED) {
// Use the largest region covered by overlaps as the trim
assert(ovlLen > 0);
assert(id == ovl[0].a_iid);
isGood = largestCovered(ovl, ovlLen,
read,
ibgn, iend, fbgn, fend,
logMsg,
errorValue,
minEvidenceOverlap,
minEvidenceCoverage,
minReadLength);
assert(fbgn <= fend);
}
else if (libr->gkLibrary_finalTrim() == FINALTRIM_BEST_EDGE) {
// Use the largest region covered by overlaps as the trim
assert(ovlLen > 0);
assert(id == ovl[0].a_iid);
isGood = bestEdge(ovl, ovlLen,
read,
ibgn, iend, fbgn, fend,
logMsg,
errorValue,
minEvidenceOverlap,
minEvidenceCoverage,
minReadLength);
assert(fbgn <= fend);
}
else {
// Do nothing. Really shouldn't get here.
assert(0);
continue;
}
// Enforce the maximum clear range
if ((isGood) && (maxClr)) {
isGood = enforceMaximumClearRange(ovl, ovlLen,
read,
ibgn, iend, fbgn, fend,
logMsg,
maxClr);
assert(fbgn <= fend);
}
//
// Trimmed. Make sense of the result, write some logs, and update the output.
//
// If bad trimming or too small, write the log and keep going.
//
if ((isGood == false) || (fend - fbgn < minReadLength)) {
outClr->setbgn(id) = fbgn;
outClr->setend(id) = fend;
outClr->setDeleted(id); // Gah, just obliterates the clear range.
fprintf(logFile, F_U32"\t"F_U32"\t"F_U32"\t"F_U32"\t"F_U32"\tDEL%s\n",
id,
ibgn, iend,
fbgn, fend,
(logMsg[0] == 0) ? "" : logMsg);
}
// If we didn't change anything, also write a log.
//
else if ((ibgn == fbgn) &&
(iend == fend)) {
fprintf(logFile, F_U32"\t"F_U32"\t"F_U32"\t"F_U32"\t"F_U32"\tNOC%s\n",
id,
ibgn, iend,
fbgn, fend,
(logMsg[0] == 0) ? "" : logMsg);
continue;
}
// Otherwise, we actually did something.
else {
outClr->setbgn(id) = fbgn;
outClr->setend(id) = fend;
fprintf(logFile, F_U32"\t"F_U32"\t"F_U32"\t"F_U32"\t"F_U32"\tMOD%s\n",
id,
ibgn, iend,
fbgn, fend,
(logMsg[0] == 0) ? "" : logMsg);
}
}
gkp->gkStore_close();
delete ovs;
delete iniClr;
delete maxClr;
delete outClr;
fclose(logFile);
fclose(sumFile);
exit(0);
}
void
ovStoreFilter::reportFate(void) {
fprintf(stderr, "overlap fate:\n");
fprintf(stderr, "%16"F_U64P" SAVE - overlaps output (for unitigging)\n", saveUTG);
fprintf(stderr, "%16"F_U64P" SAVE - overlaps output (for OBT)\n", saveOBT);
fprintf(stderr, "%16"F_U64P" SAVE - overlaps output (for dedupe)\n", saveDUP);
fprintf(stderr, "\n");
fprintf(stderr, "%16"F_U64P" ERATE - low quality, more than %.3f fraction error\n", skipERATE, AS_OVS_decodeEvalue(maxEvalue));
fprintf(stderr, "\n");
fprintf(stderr, "%16"F_U64P" OBT - not requested\n", skipOBT);
fprintf(stderr, "%16"F_U64P" OBT - too similar\n", skipOBTbad);
fprintf(stderr, "%16"F_U64P" OBT - too short\n", skipOBTshort);
fprintf(stderr, "\n");
fprintf(stderr, "%16"F_U64P" DUP - dedupe not requested\n", skipDUP);
fprintf(stderr, "%16"F_U64P" DUP - different library\n", skipDUPlib);
fprintf(stderr, "%16"F_U64P" DUP - obviously not duplicates\n", skipDUPdiff);
}
