HHInfo *CreateHelpViewer(LPCWSTR filename)
{
HHInfo *info = heap_alloc_zero(sizeof(HHInfo));
int i;
/* Set the invalid tab ID (-1) as the default value for all
* of the tabs, this matches a failed TCM_INSERTITEM call.
*/
for(i=0;i<sizeof(info->tabs)/sizeof(HHTab);i++)
info->tabs[i].id = -1;
OleInitialize(NULL);
info->pCHMInfo = OpenCHM(filename);
if(!info->pCHMInfo) {
ReleaseHelpViewer(info);
return NULL;
}
if (!LoadWinTypeFromCHM(info)) {
ReleaseHelpViewer(info);
return NULL;
}
if(!CreateViewer(info)) {
ReleaseHelpViewer(info);
return NULL;
}
return info;
}
static void ConsignProc (ButtoN b)
{
XOSPtr xosp;
XISPtr xisp;
ComPatPtr cpp, cpph;
ValNodePtr orflist;
SeqLocPtr slp, slpn;
Int4 start, stop;
Uint1 strand;
SeqPortPtr spp;
Uint1Ptr aaseq;
Int4 ntpos, aapos;
Uint1 cdn[3];
SeqAlignPtr sap, sapn;
FloatHi probcut;
Int4 clustmin, findmin;
Int4 i, n, endpos, XLength, XScale, shift;
Int4 iframe, frame, top, orftop[6];
FloatHiPtr score, expandscore;
FloatHi maxscore;
Int4Ptr tableGlobal;
SeqGraphPtr sgp, sgpn;
WindoW w;
VieweR v;
GrouP g;
SegmenT seg;
GraphSentPtr gsp;
Char numberbuffer[32];
if ((xosp = (XOSPtr) GetObjectExtra (b)) == NULL)
return;
if (xosp->bsp == NULL)
{
ErrPostEx (SEV_ERROR, TOP_ERROR, 101, "No Bioseq");
ErrShow ();
return;
}
WatchCursor ();
cpph = cpp = ReadPrositePattern (xosp->pattern_file, TRUE, -1, NULL, NULL);
if (cpph == NULL)
{
ErrPostEx (SEV_ERROR, TOP_ERROR, 101,
"read failed %s", xosp->pattern_file);
ErrShow ();
ArrowCursor ();
return;
}
xosp->orflist = GetOrfList (xosp->bsp, (Int2) (xosp->orfcut));
xosp->orflist = ClearNonMetOrfs (xosp->orflist);
orflist = xosp->orflist;
while (orflist != NULL)
{
slp = (SeqLocPtr) orflist->data.ptrvalue;
if (slp->choice == 0)
{
orflist = orflist->next;
continue;
}
if (slp->choice == SEQLOC_MIX)
slp = (SeqLocPtr) slp->data.ptrvalue;
start = SeqLocStart (slp);
stop = SeqLocStop (slp);
strand = SeqLocStrand (slp);
if (strand != Seq_strand_both)
strand = Seq_strand_both;
if (stop - start + 1 >= xosp->minimumseed)
{
spp = SeqPortNew (xosp->bsp, start, stop, strand, Seq_code_ncbi4na);
aaseq = (Uint1Ptr) MemNew ((size_t)
(sizeof (Uint1) * (((stop-start)/3)+2)));
ntpos = start;
aapos = 0;
while (ntpos < start+3)
{
cdn[0] = SeqPortGetResidue (spp);
ntpos++;
cdn[1] = SeqPortGetResidue (spp);
ntpos++;
cdn[2] = SeqPortGetResidue (spp);
ntpos++;
aaseq[aapos] = AAForCodon (cdn, xosp->gcdi);
aapos++;
}
while (ntpos <= stop)
{
cdn[0] = SeqPortGetResidue (spp);
ntpos++;
cdn[1] = SeqPortGetResidue (spp);
ntpos++;
cdn[2] = SeqPortGetResidue (spp);
ntpos++;
aaseq[aapos] = AAForCodon (cdn, xosp->gcd);
aapos++;
}
SeqPortFree (spp);
aaseq[aapos] = 0;
cpp = cpph;
while (cpp != NULL)
{
sap = PatternMatch (aaseq, 0, Seq_strand_plus, SeqLocId (slp),
cpp, 0, Seq_strand_unknown, FALSE);
if (sap != NULL)
break;
cpp = cpp->nextpattern;
}
MemFree (aaseq);
if (sap != NULL)
{
SeqLocLink (&(xosp->slps), SeqLocDup (slp));
}
while (sap != NULL)
{
sapn = sap->next;
SeqAlignFree (sap);
sap = sapn;
}
}
orflist = orflist->next;
}
ComPatFree (cpph);
orflist = xosp->orflist;
while (orflist != NULL)
{
slp = (SeqLocPtr) orflist->data.ptrvalue;
if (slp->choice > 0)
SeqLocLink (&(xosp->slpa), SeqLocDup (slp));
while (slp != NULL)
{
slpn = slp->next;
SeqLocFree (slp);
slp = slpn;
}
orflist->data.ptrvalue = NULL;
orflist = orflist->next;
}
xosp->orflist = ValNodeFree (xosp->orflist);
probcut = xosp->probcut;
clustmin = xosp->clustmin;
findmin = xosp->findmin;
xosp->slpb = FindSimilarBiasOrfs (xosp->sep, probcut, clustmin, findmin,
xosp->slps, xosp->slpa);
tableGlobal = CodonTableFromSeqLoc (xosp->bsp, xosp->slpb);
seg = NULL;
top = 0;
xisp = (XISPtr) MemNew (sizeof (XIS));
frame = 0;
for (iframe = 0; iframe < 6; iframe++)
{
endpos = (xosp->bsp->length + 3 - frame - xosp->window) / 3;
if (iframe < 3)
score = BiasScoreBioseq (xosp->bsp, tableGlobal, xosp->window,
frame, Seq_strand_plus);
else
score = BiasScoreBioseq (xosp->bsp, tableGlobal, xosp->window,
frame, Seq_strand_minus);
maxscore = 0.0;
for (i = 0; i < endpos; i++)
if (score[i] > maxscore)
maxscore = score[i];
expandscore = (FloatHiPtr) MemNew (sizeof (FloatHi) * xosp->bsp->length);
for (i = 0; i < xosp->window/2; i++)
expandscore[i] = maxscore;
n = 0;
while (i < xosp->bsp->length)
{
if (n < endpos)
expandscore[i] = score[n];
else
expandscore[i] = maxscore;
i++;
if (i%3 == 0)
n++;
}
MemFree (score);
score = expandscore;
sgp = SeqGraphNew ();
if (xisp->sgp == NULL)
{
xisp->sgp = sgp;
}
else
{
sgpn = xisp->sgp;
while (sgpn->next != NULL)
sgpn = sgpn->next;
sgpn->next = sgp;
}
XLength = xosp->bsp->length;
if (XLength > 1200)
XLength = 1200;
XScale = xosp->bsp->length / XLength;
if (xosp->bsp->length % XLength != 0)
XScale++;
sgp->loc = SeqLocIntNew (0, xosp->bsp->length-1, xosp->bsp->strand,
xosp->bsp->id);
sgp->flags[2] = 1;
sgp->numval = xosp->bsp->length;
sgp->values = (Pointer) score;
sgp->max.realvalue = maxscore;
sgp->min.realvalue = 0.0;
sgp->flags[1] = 1;
sgp->a = 4.0;
sgp->b = 0.0;
if (seg == NULL)
seg = CreatePicture ();
if ((gsp = AddGraphSentinelToPicture (sgp, xosp->bsp, seg, 0,
top, 0, NULL)) != NULL)
{
sprintf (numberbuffer, "%ld", 1L);
AddLabel (seg, gsp->box.left, gsp->bottom-20,
numberbuffer, SMALL_TEXT, 0, MIDDLE_CENTER, 0);
sprintf (numberbuffer, "%ld", (long) xosp->bsp->length);
AddLabel (seg, gsp->box.left+xosp->bsp->length, gsp->bottom-20,
numberbuffer, SMALL_TEXT, 0, MIDDLE_CENTER, 0);
}
shift = (Int4) (maxscore*sgp->a);
orftop[iframe] = top - shift - 38;
top -= (shift+56);
frame++;
if (frame == 3)
{
top -= 24;
frame = 0;
}
}
frame = 0;
for (iframe = 0; iframe < 6; iframe++)
{
if (iframe < 3)
strand = Seq_strand_plus;
else
strand = Seq_strand_minus;
shift = 0;
if (xosp->slpa != NULL)
{
AddOrfClass (xosp->slpa, seg, orftop, iframe, frame,
shift, strand, YELLOW_COLOR, 5);
shift += 4;
}
if (xosp->slpk != NULL)
{
AddOrfClass (xosp->slpk, seg, orftop, iframe, frame,
shift, strand, GREEN_COLOR, 5);
shift += 4;
}
if (xosp->slpb != NULL)
{
AddOrfClass (xosp->slpb, seg, orftop, iframe, frame,
shift, strand, BLUE_COLOR, 5);
shift += 4;
}
if (xosp->slps != NULL)
{
AddOrfClass (xosp->slps, seg, orftop, iframe, frame,
shift, strand, RED_COLOR, 5);
}
frame++;
if (frame == 3)
frame = 0;
}
MemFree (tableGlobal);
start = 20;
stop = 20 + (50*XScale);
top = orftop[5] - 40;
if (xosp->slpa != NULL)
{
top -= 12;
AddAttribute (seg, (COLOR_ATT|STYLE_ATT|SHADING_ATT|WIDTH_ATT),
YELLOW_COLOR, SOLID_LINE, SOLID_SHADING, 5,
0);
AddLine (seg, start, top, stop, top, FALSE, 0);
AddAttribute (seg, (COLOR_ATT|STYLE_ATT|SHADING_ATT|WIDTH_ATT),
BLACK_COLOR, SOLID_LINE, SOLID_SHADING, STD_PEN_WIDTH,
0);
AddLabel (seg, stop+(20*XScale), top,
"All Met-init'd ORFs equal to or greater than 50 codons",
SMALL_TEXT, 0, MIDDLE_RIGHT, 0);
}
if (xosp->slpk != NULL)
{
top -= 12;
AddAttribute (seg, (COLOR_ATT|STYLE_ATT|SHADING_ATT|WIDTH_ATT),
GREEN_COLOR, SOLID_LINE, SOLID_SHADING, 5,
0);
AddLine (seg, start, top, stop, top, FALSE, 0);
AddAttribute (seg, (COLOR_ATT|STYLE_ATT|SHADING_ATT|WIDTH_ATT),
BLACK_COLOR, SOLID_LINE, SOLID_SHADING, STD_PEN_WIDTH,
0);
AddLabel (seg, stop+(20*XScale), top, "Annotated (reported) ORFs",
SMALL_TEXT, 0, MIDDLE_RIGHT, 0);
}
if (xosp->slpb != NULL)
{
top -= 12;
AddAttribute (seg, (COLOR_ATT|STYLE_ATT|SHADING_ATT|WIDTH_ATT),
BLUE_COLOR, SOLID_LINE, SOLID_SHADING, 5,
0);
AddLine (seg, start, top, stop, top, FALSE, 0);
AddAttribute (seg, (COLOR_ATT|STYLE_ATT|SHADING_ATT|WIDTH_ATT),
BLACK_COLOR, SOLID_LINE, SOLID_SHADING, STD_PEN_WIDTH,
0);
AddLabel (seg, stop+(20*XScale), top, "Similar codon usage bias ORFs to seed ORFs",
SMALL_TEXT, 0, MIDDLE_RIGHT, 0);
}
if (xosp->slps != NULL)
{
top -= 12;
AddAttribute (seg, (COLOR_ATT|STYLE_ATT|SHADING_ATT|WIDTH_ATT),
RED_COLOR, SOLID_LINE, SOLID_SHADING, 5,
0);
AddLine (seg, start, top, stop, top, FALSE, 0);
AddAttribute (seg, (COLOR_ATT|STYLE_ATT|SHADING_ATT|WIDTH_ATT),
BLACK_COLOR, SOLID_LINE, SOLID_SHADING, STD_PEN_WIDTH,
0);
AddLabel (seg, stop+(20*XScale), top, "Pattern match seed ORFs",
SMALL_TEXT, 0, MIDDLE_RIGHT, 0);
}
xisp->picture = seg;
w = FixedWindow (10, 10, 640, 720, "Consign", CloseGraphWindowProc);
SetObjectExtra (w, xisp, CleanUpGraphWindow);
g = HiddenGroup (w, -1, 0, NULL);
v = CreateViewer (g, 560, 640, TRUE, TRUE);
AttachPicture (v, seg, INT4_MIN, INT4_MAX, UPPER_LEFT, XScale, 1, NULL);
PushButton (g, "Close", CloseGraphWindowButton);
RealizeWindow (w);
ArrowCursor ();
Show (w);
return;
}
static void ConsortProc (ButtoN b)
{
XOSPtr xosp;
XISPtr xisp;
TreeNodePtr ptrNode;
WindoW w;
VieweR v;
GrouP g;
SegmenT seg;
Int4 xmax, xmin;
Int4 xoffset, yoffset;
if ((xosp = (XOSPtr) GetObjectExtra (b)) == NULL)
return;
if (xosp->sep == NULL)
{
ErrPostEx (SEV_ERROR, TOP_ERROR, 101, "No SeqEntry");
ErrShow ();
return;
}
WatchCursor ();
xoffset = xosp->xdeltaval;
yoffset = xosp->ydeltaval;
ptrNode = ConsortSeqEntry (xosp->sep);
SetAllLeftRightRootedTreeNeighbors (ptrNode);
SetAllPivotNodes (ptrNode);
switch (xosp->treestyle)
{
default:
case 0:
SetAllTheoreticalCoordinates (ptrNode, 300, 50);
AdjustAllTheoreticalCoordinates (ptrNode, 300);
ChangeTreeScale (ptrNode, 300, 50, (FloatHi) xoffset, (FloatHi) yoffset);
break;
case 1:
SetAllCoordinates (ptrNode, 300, 50, xoffset, yoffset);
break;
}
xmin = GetMinimumTreeXcoord (ptrNode);
if (xmin-50 > 0)
ShiftTree (ptrNode, (-1*(xmin-50)), 0);
if (xmin < 0)
ShiftTree (ptrNode, ((-1*xmin)+50), 0);
xmin = GetMinimumTreeXcoord (ptrNode);
xmax = GetMaximumTreeXcoord (ptrNode);
if (xmin < 0)
xmin *= -1;
if (xmax < 0)
xmax *= -1;
xisp = (XISPtr) MemNew (sizeof (XIS));
if (xmax > xmin)
xisp->Xdelta = (2*(xmax+100)) + 10;
else
xisp->Xdelta = (2*(xmin+100)) + 10;
seg = DrawTree (ptrNode);
xisp->picture = seg;
w = FixedWindow (10, 10, 640, 640, "Consort", CloseGraphWindowProc);
SetObjectExtra (w, xisp, CleanUpGraphWindow);
g = HiddenGroup (w, -1, 0, NULL);
v = CreateViewer (g, 560, 560, TRUE, TRUE);
AttachPicture (v, seg, INT4_MIN, INT4_MAX, UPPER_LEFT, 1, 1, NULL);
PushButton (g, "Close", CloseGraphWindowButton);
RealizeWindow (w);
ArrowCursor ();
Show (w);
return;
}
