static void hgSeqDisplayOptions(struct cart *cart, boolean canDoUTR,
boolean canDoIntrons, boolean offerRevComp)
/* Print out HTML FORM entries for sequence display options. */
{
char *casing, *repMasking;
puts("\n<H3> Sequence Formatting Options: </H3>\n");
casing = cartCgiUsualString(cart, "hgSeq.casing", "exon");
if (canDoIntrons)
{
cgiMakeRadioButton("hgSeq.casing", "exon", sameString(casing, "exon"));
if (canDoUTR)
puts("Exons in upper case, everything else in lower case. <BR>");
else
puts("Blocks in upper case, everything else in lower case. <BR>");
}
if (canDoUTR)
{
if (sameString(casing, "exon") && !canDoIntrons)
casing = "cds";
cgiMakeRadioButton("hgSeq.casing", "cds", sameString(casing, "cds"));
puts("CDS in upper case, UTR in lower case. <BR>");
}
if ((sameString(casing, "exon") && !canDoIntrons) ||
(sameString(casing, "cds") && !canDoUTR))
casing = "upper";
cgiMakeRadioButton("hgSeq.casing", "upper", sameString(casing, "upper"));
puts("All upper case. <BR>");
cgiMakeRadioButton("hgSeq.casing", "lower", sameString(casing, "lower"));
puts("All lower case. <BR>");
cgiMakeCheckBox("hgSeq.maskRepeats",
cartCgiUsualBoolean(cart, "hgSeq.maskRepeats", FALSE));
puts("Mask repeats: ");
repMasking = cartCgiUsualString(cart, "hgSeq.repMasking", "lower");
cgiMakeRadioButton("hgSeq.repMasking", "lower",
sameString(repMasking, "lower"));
puts(" to lower case ");
cgiMakeRadioButton("hgSeq.repMasking", "N", sameString(repMasking, "N"));
puts(" to N <BR>");
if (offerRevComp)
{
cgiMakeCheckBox("hgSeq.revComp",
cartCgiUsualBoolean(cart, "hgSeq.revComp", FALSE));
puts("Reverse complement (get \'-\' strand sequence)");
}
}
Bits *bitsForIntersectingTable(struct sqlConnection *conn, struct region *region, int chromSize,
boolean isBpWise)
/* Get a bitmap that corresponds to the table we are intersecting with.
* Consult CGI vars to figure out what table it is. */
{
boolean invTable2 = cartCgiUsualBoolean(cart, hgtaInvertTable2, FALSE);
char *table2 = cartString(cart, hgtaIntersectTable);
struct hTableInfo *hti2 = getHti(database, table2, conn);
struct lm *lm2 = lmInit(64*1024);
Bits *bits2 = bitAlloc(chromSize+8);
struct bed *bedList2;
if (isBigWigTable(table2))
bedList2 = bigWigIntervalsToBed(conn, table2, region, lm2);
else
// We should go straight to raw beds here, not through the routines that
// do filter & intersections, because the secondary table has no filter
// and sure shouldn't be intersected. :)
bedList2 = getFilteredBeds(conn, table2, region, lm2, NULL);
if (!isBpWise)
expandZeroSize(bedList2, hti2->hasBlocks, chromSize);
bedOrBits(bits2, chromSize, bedList2, hti2->hasBlocks, 0);
if (invTable2)
bitNot(bits2, chromSize);
lmCleanup(&lm2);
return bits2;
}
static struct bed *bedTable2(struct sqlConnection *conn,
struct region *region, char *table2)
/* get a bed list, possibly complement, for table2 */
{
/* This use of bedTable rather than a bitmap is not really working. The
* rest of the table browser does intersection at the exon level, while
* the wig code, which this is part of, does it at the gene level. I
* noticed it while working on the corresponding routines for bigWig,
* which I'm building to work with bitmaps at the exon level. I'm not
* sure it's worth fixing this code since nobody has complained, and we're
* probably going to be doing mostly bigWig rather than wig in the future.
* -JK */
boolean invTable2 = cartCgiUsualBoolean(cart, hgtaInvertTable2, FALSE);
char *op = cartString(cart, hgtaIntersectOp);
struct bed *bedList = NULL;
struct lm *lm1 = lmInit(64*1024);
/* fetch table 2 as a bed list */
bedList = getFilteredBeds(conn, table2, region, lm1, NULL);
/* If table 2 bed list needs to be complemented (!table2), then do so */
if (invTable2 || sameString("none", op))
{
unsigned chromStart = 0; /* start == end == 0 */
unsigned chromEnd = 0; /* means do full chrom */
unsigned chromSize = hChromSize(database, region->chrom);
struct lm *lm2 = lmInit(64*1024);
struct bed *inverseBedList = NULL; /* new list */
if ((region->start != 0) || (region->end != 0))
{
chromStart = region->start;
chromEnd = region->end;
}
if ((struct bed *)NULL == bedList)
{
if (0 == region->end)
chromEnd = chromSize;
addBedElement(&inverseBedList, region->chrom, chromStart, chromEnd,
1, lm2);
}
else
inverseBedList=invertBedList(bedList, lm2, region->chrom, chromStart,
chromEnd, chromSize);
lmCleanup(&lm1); /* == bedFreeList(&bedList) */
return inverseBedList;
}
else
return bedList;
}
Bits *bitsForIntersectingTable(struct sqlConnection *conn, struct region *region, int chromSize,
boolean isBpWise)
/* Get a bitmap that corresponds to the table we are intersecting with.
* Consult CGI vars to figure out what table it is. */
{
boolean invTable2 = cartCgiUsualBoolean(cart, hgtaInvertTable2, FALSE);
char *table2 = cartString(cart, hgtaIntersectTable);
struct hTableInfo *hti2 = getHti(database, table2, conn);
struct lm *lm2 = lmInit(64*1024);
Bits *bits2 = bitAlloc(chromSize+8);
struct bed *bedList2 = getFilteredBeds(conn, table2, region, lm2, NULL);
if (!isBpWise)
expandZeroSize(bedList2, hti2->hasBlocks, chromSize);
bedOrBits(bits2, chromSize, bedList2, hti2->hasBlocks, 0);
if (invTable2)
bitNot(bits2, chromSize);
lmCleanup(&lm2);
return bits2;
}
void doBedOrCtOptions(char *table, struct sqlConnection *conn,
boolean doCt)
/* Put up form to get options on BED or custom track output. */
/* (Taken from hgText.c/doBedCtOptions) */
{
char *table2 = NULL; /* For now... */
struct hTableInfo *hti = getHti(database, table, conn);
char buf[256];
char *setting;
htmlOpen("Output %s as %s", table, (doCt ? "Custom Track" : "BED"));
if (doGalaxy())
startGalaxyForm();
else if (doGreat())
{
verifyGreatAssemblies();
startGreatForm();
}
else
hPrintf("<FORM ACTION=\"%s\" METHOD=GET>\n", getScriptName());
cartSaveSession(cart);
if (!doGreat())
{
hPrintf("%s\n", "<TABLE><TR><TD>");
if (doCt)
{
hPrintf("%s\n", "</TD><TD>"
"<A HREF=\"../goldenPath/help/customTrack.html\" TARGET=_blank>"
"Custom track</A> header: </B>");
}
else
{
cgiMakeCheckBox(hgtaPrintCustomTrackHeaders,
cartCgiUsualBoolean(cart, hgtaPrintCustomTrackHeaders, FALSE));
hPrintf("%s\n", "</TD><TD> <B> Include "
"<A HREF=\"../goldenPath/help/customTrack.html\" TARGET=_blank>"
"custom track</A> header: </B>");
}
hPrintf("%s\n", "</TD></TR><TR><TD></TD><TD>name=");
safef(buf, sizeof(buf), "tb_%s", hti->rootName);
setting = cgiUsualString(hgtaCtName, buf);
cgiMakeTextVar(hgtaCtName, setting, 16);
hPrintf("%s\n", "</TD></TR><TR><TD></TD><TD>description=");
safef(buf, sizeof(buf), "table browser query on %s%s%s",
table, (table2 ? ", " : ""), (table2 ? table2 : ""));
setting = cgiUsualString(hgtaCtDesc, buf);
cgiMakeTextVar(hgtaCtDesc, setting, 50);
hPrintf("%s\n", "</TD></TR><TR><TD></TD><TD>visibility=");
if (isWiggle(database, table) || isBigWigTable(table))
{
setting = cartCgiUsualString(cart, hgtaCtVis, ctVisWigMenu[2]);
cgiMakeDropList(hgtaCtVis, ctVisWigMenu, ctVisWigMenuSize, setting);
}
else
{
setting = cartCgiUsualString(cart, hgtaCtVis, ctVisMenu[3]);
cgiMakeDropList(hgtaCtVis, ctVisMenu, ctVisMenuSize, setting);
}
hPrintf("%s\n", "</TD></TR><TR><TD></TD><TD>url=");
setting = cartCgiUsualString(cart, hgtaCtUrl, "");
cgiMakeTextVar(hgtaCtUrl, setting, 50);
hPrintf("%s\n", "</TD></TR><TR><TD></TD><TD>");
hPrintf("%s\n", "</TD></TR></TABLE>");
}
if (isWiggle(database, table) || isBedGraph(table) || isBigWigTable(table) )
{
char *setting = NULL;
hPrintf("<P> <B> Select type of data output: </B> <BR>\n");
setting = cartCgiUsualString(cart, hgtaCtWigOutType, outWigData);
cgiMakeRadioButton(hgtaCtWigOutType, outWigBed, sameString(setting, outWigBed));
hPrintf("BED format (no data value information, only position)<BR>\n");
cgiMakeRadioButton(hgtaCtWigOutType, outWigData, sameString(setting, outWigData));
hPrintf("DATA VALUE format (position and real valued data)</P>\n");
}
else
{
cgiDown(0.9);
hPrintf("<B> Create one BED record per: </B>\n");
if ((anyIntersection() && intersectionIsBpWise()) ||
(anySubtrackMerge(database, table) && subtrackMergeIsBpWise()))
{
/* The original table may have blocks/CDS, described in hti, but
* that info will be lost after base pair-wise operations. So make
* a temporary copy of hti with its flags tweaked: */
struct hTableInfo simplifiedHti;
memcpy(&simplifiedHti, hti, sizeof(simplifiedHti));
simplifiedHti.hasBlocks = FALSE;
simplifiedHti.hasCDS = FALSE;
fbOptionsHtiCart(&simplifiedHti, cart);
}
else
fbOptionsHtiCart(hti, cart);
}
if (doCt)
{
if (doGalaxy())
{
/* send the action parameter with the form as well */
cgiMakeHiddenVar(hgtaDoGetCustomTrackFile, "get custom track in file");
printGalaxySubmitButtons();
}
else
{
cgiMakeButton(hgtaDoGetCustomTrackTb, "get custom track in table browser");
hPrintf(" ");
cgiMakeButton(hgtaDoGetCustomTrackFile, "get custom track in file");
hPrintf("<BR>\n");
cgiMakeButton(hgtaDoGetCustomTrackGb, "get custom track in genome browser");
}
}
else
{
if (doGalaxy())
{
cgiMakeHiddenVar(hgtaDoGetBed, "get BED");
printGalaxySubmitButtons();
}
else if (doGreat())
{
cgiMakeHiddenVar(hgtaDoGetBed, "get BED");
printGreatSubmitButtons();
}
else
cgiMakeButton(hgtaDoGetBed, "get BED");
}
if (!doGalaxy() && !doGreat())
{
hPrintf(" ");
cgiMakeButton(hgtaDoMainPage, "cancel");
hPrintf("</FORM>\n");
}
cgiDown(0.9);
htmlClose();
}
static struct bed *intersectOnRegion(
struct sqlConnection *conn, /* Open connection to database. */
struct region *region, /* Region to work inside */
char *table1, /* Table input list is from. */
struct bed *bedList1, /* List before intersection, should be
* all within region. */
struct lm *lm, /* Local memory pool. */
int *retFieldCount) /* Field count. */
/* Intersect bed list, consulting CGI vars to figure out
* with what table and how. Return intersected result,
* which is independent from input. This potentially will
* chew up bedList1. */
{
/* Grab parameters for intersection from cart. */
double moreThresh = cartCgiUsualDouble(cart, hgtaMoreThreshold, 0);
double lessThresh = cartCgiUsualDouble(cart, hgtaLessThreshold, 100);
boolean invTable = cartCgiUsualBoolean(cart, hgtaInvertTable, FALSE);
char *op = intersectOp();
/* --- TODO MIKE - replace bedList2, bits2 with baseMask stuff. */
/* Load up intersecting bedList2 (to intersect with) */
int chromSize = hChromSize(database, region->chrom);
boolean isBpWise = (sameString("and", op) || sameString("or", op));
Bits *bits2 = bitsForIntersectingTable(conn, region, chromSize, isBpWise);
/* Set up some other local vars. */
struct hTableInfo *hti1 = getHti(database, table1, conn);
struct bed *intersectedBedList = NULL;
/* Produce intersectedBedList. */
if (isBpWise)
{
/* --- TODO MIKE - replace, bits1 with baseMask stuff. */
/* Base-pair-wise operation: get bitmap for primary table too */
Bits *bits1 = bitAlloc(chromSize+8);
boolean hasBlocks = hti1->hasBlocks;
if (retFieldCount != NULL && (*retFieldCount < 12))
hasBlocks = FALSE;
bedOrBits(bits1, chromSize, bedList1, hasBlocks, 0);
/* invert inputs if necessary */
if (invTable)
bitNot(bits1, chromSize);
/* do the intersection/union */
if (sameString("and", op))
bitAnd(bits1, bits2, chromSize);
else
bitOr(bits1, bits2, chromSize);
/* clip to region if necessary: */
if (region->start > 0)
bitClearRange(bits1, 0, region->start);
if (region->end < chromSize)
bitClearRange(bits1, region->end, (chromSize - region->end));
/* translate back to bed */
intersectedBedList = bitsToBed4List(bits1, chromSize,
region->chrom, 1, region->start, region->end, lm);
if (retFieldCount != NULL)
*retFieldCount = 4;
bitFree(&bits1);
}
else
intersectedBedList = filterBedByOverlap(bedList1, hti1->hasBlocks, op,
moreThresh, lessThresh, bits2,
chromSize);
bitFree(&bits2);
return intersectedBedList;
}
void mafPrettyOut(FILE *f, struct mafAli *maf, int lineSize,
boolean onlyDiff, int blockNo)
{
int ii, ch;
int srcChars = 0;
struct mafComp *mc;
int lineStart, lineEnd;
char *summaryLine = needMem(lineSize+1);
char *referenceText;
int startChars, sizeChars, srcSizeChars;
boolean haveInserts = FALSE;
struct mafComp *masterMc = maf->components;
startChars = sizeChars = srcSizeChars = 0;
for (mc = maf->components; mc != NULL; mc = mc->next)
{
/* Figure out length of source (species) field. */
/*if (mc->size != 0)*/
{
char dbOnly[128];
int len;
char *org;
memset(dbOnly, 0, sizeof(dbOnly));
safef(dbOnly, sizeof(dbOnly), "%s", mc->src);
chopPrefix(dbOnly);
if ((org = hOrganism(dbOnly)) == NULL)
len = strlen(dbOnly);
else
len = strlen(org);
if (srcChars < len)
srcChars = len;
len = digitsBaseTen(mc->start);
if (startChars < len)
startChars = len;
len = digitsBaseTen(mc->size);
if (sizeChars < len)
sizeChars = len;
len = digitsBaseTen(mc->srcSize);
if (srcSizeChars < len)
srcSizeChars = len;
if (mc->text && (mc->rightStatus == MAF_INSERT_STATUS) && (masterMc->start + masterMc->size < winEnd))
haveInserts = TRUE;
#ifdef REVERSESTRAND
/* complement bases if hgTracks is on reverse strand */
if (mc->size && cartCgiUsualBoolean(cart, COMPLEMENT_BASES_VAR, FALSE))
complement(mc->text, maf->textSize);
#endif
}
}
/* first sequence in the alignment */
referenceText = maf->components->text;
for (lineStart = 0; lineStart < maf->textSize; lineStart = lineEnd)
{
int size;
lineEnd = lineStart + lineSize;
if (lineEnd >= maf->textSize)
lineEnd = maf->textSize;
size = lineEnd - lineStart;
initSummaryLine(summaryLine, size, '*');
for (mc = maf->components; mc != NULL; mc = mc->next)
{
char dbOnly[128], *chrom;
int s = mc->start;
int e = s + mc->size;
char *org;
char *revComp = "";
char strand = mc->strand;
struct dyString *dy = newDyString(512);
#ifdef REVERSESTRAND
if (cartCgiUsualBoolean(cart, COMPLEMENT_BASES_VAR, FALSE))
strand = (strand == '+') ? '-' : '+';
#endif
if (strand == '-') revComp = "&hgSeq.revComp=on";
dyStringClear(dy);
safef(dbOnly, sizeof(dbOnly), "%s", mc->src);
chrom = chopPrefix(dbOnly);
if ((org = hOrganism(dbOnly)) == NULL)
org = dbOnly;
if (mc->strand == '-')
reverseIntRange(&s, &e, mc->srcSize);
if (mc->text != NULL)
{
if (lineStart == 0)
{
if (hDbIsActive(dbOnly))
{
dyStringPrintf(dy, "%s Browser %s:%d-%d %c %*dbps",hOrganism(dbOnly),chrom, s+1, e, mc->strand,sizeChars, mc->size);
linkToOtherBrowserTitle(dbOnly, chrom, s, e, dy->string);
dyStringClear(dy);
fprintf(f, "B</A> ");
}
else
fprintf(f, " ");
if (hDbExists(dbOnly))
{
dyStringPrintf(dy, "Get %s DNA %s:%d-%d %c %*dbps",hOrganism(dbOnly),chrom, s+1, e, mc->strand,sizeChars, mc->size);
printf("<A TITLE=\"%s\" TARGET=\"_blank\" HREF=\"%s?o=%d&g=getDna&i=%s&c=%s&l=%d&r=%d&db=%s%s\">D</A> ", dy->string,hgcName(),
s, cgiEncode(chrom),
chrom, s, e, dbOnly, revComp);
}
else
fprintf(f, " ");
}
else
{
fprintf(f, " ");
}
dyStringClear(dy);
dyStringPrintf(dy, "%s:%d-%d %c %*dbps",chrom, s+1, e, mc->strand,sizeChars, mc->size);
fprintf(f, "<A TITLE=\"%s\"> %*s </A> ", dy->string, srcChars, org);
updateSummaryLine(summaryLine, referenceText + lineStart, mc->text + lineStart, size);
blueCapWrite(f, mc->text + lineStart, size,
(onlyDiff && mc != maf->components) ? referenceText + lineStart : NULL);
fprintf(f, "\n");
}
else
{
if (((mc->leftStatus == MAF_CONTIG_STATUS) && (mc->rightStatus == MAF_CONTIG_STATUS) )
|| ((mc->leftStatus == MAF_TANDEM_STATUS) && (mc->rightStatus == MAF_TANDEM_STATUS) )
|| ((mc->leftStatus == MAF_INSERT_STATUS) && (mc->rightStatus == MAF_INSERT_STATUS) )
|| ((mc->leftStatus == MAF_MISSING_STATUS) && (mc->rightStatus == MAF_MISSING_STATUS) ))
{
if (lineStart == 0)
{
int s = mc->start;
int e = s + mc->rightLen;
struct dyString *dy = newDyString(512);
if (mc->strand == '-')
reverseIntRange(&s, &e, mc->srcSize);
if ( hDbIsActive(dbOnly))
{
dyStringPrintf(dy, "%s Browser %s:%d-%d %c %d bps Unaligned",hOrganism(dbOnly),chrom, s+1, e, mc->strand, e-s);
linkToOtherBrowserTitle(dbOnly, chrom, s, e, dy->string);
fprintf(f,"B</A> ");
dyStringClear(dy);
}
else
fprintf(f," ");
if (hDbExists(dbOnly))
{
dyStringPrintf(dy, "Get %s DNA %s:%d-%d %c %d bps Unaligned",hOrganism(dbOnly),chrom, s+1, e, mc->strand, e-s);
printf("<A TITLE=\"%s\" TARGET=\"_blank\" HREF=\"%s?o=%d&g=getDna&i=%s&c=%s&l=%d&r=%d&db=%s%s\">D</A> ", dy->string, hgcName(),
s, cgiEncode(chrom),
chrom, s, e, dbOnly,revComp);
}
else
fprintf(f, " ");
}
else
fprintf(f, " ");
initSummaryLine(summaryLine, size, ' ');
dyStringClear(dy);
dyStringPrintf(dy, "%s:%d-%d %c %*dbps",chrom, s+1, e, mc->strand,sizeChars, mc->size);
fprintf(f, "<A TITLE=\"%s\">%*s</A> ", dy->string, srcChars, org);
ch = '-';
switch(mc->rightStatus)
{
case MAF_INSERT_STATUS:
ch = '=';
break;
case MAF_MISSING_STATUS:
ch = 'N';
break;
case MAF_TANDEM_STATUS:
case MAF_CONTIG_STATUS:
ch = '-';
break;
}
for(ii=lineStart; ii < lineEnd ; ii++)
fputc(ch,f);
fprintf(f,"\n");
}
}
}
#ifdef ADDMATCHLINE
if (lineStart == 0)
fprintf(f, " %-*s %s\n", srcChars, "", summaryLine);
else
fprintf(f, "%-*s %s\n", srcChars, "", summaryLine);
#else
fprintf(f, "\n");
#endif
}
if (haveInserts)
{
fprintf(f, "<B>Inserts between block %d and %d in window</B>\n",blockNo, blockNo+1);
for (mc = maf->components; mc != NULL; mc = mc->next)
{
char dbOnly[128], *chrom;
int s = mc->start + mc->size;
int e = s + mc->rightLen;
char *org;
if (mc->text == NULL)
continue;
if (mc->strand == '-')
reverseIntRange(&s, &e, mc->srcSize);
safef(dbOnly, sizeof(dbOnly), "%s", mc->src);
chrom = chopPrefix(dbOnly);
if ((org = hOrganism(dbOnly)) == NULL)
org = dbOnly;
if (mc->rightStatus == MAF_INSERT_STATUS)
{
char *revComp = "";
if (hDbIsActive(dbOnly))
{
char strand = mc->strand;
#ifdef REVERSESTRAND
if (cartCgiUsualBoolean(cart, COMPLEMENT_BASES_VAR, FALSE))
strand = (strand == '+') ? '-' : '+';
#endif
if (strand == '-') revComp = "&hgSeq.revComp=on";
linkToOtherBrowser(dbOnly, chrom, s, e);
fprintf(f,"B");
fprintf(f, "</A>");
fprintf(f, " ");
}
else
fprintf(f, " ");
if (hDbExists(dbOnly))
{
printf("<A TARGET=\"_blank\" HREF=\"%s?o=%d&g=getDna&i=%s&c=%s&l=%d&r=%d"
"&db=%s%s\">D</A> ", hgcName(), s, cgiEncode(chrom), chrom, s,
e, dbOnly,revComp);
}
else
fprintf(f, " ");
fprintf(f, "%*s %dbp\n", srcChars, org,mc->rightLen);
}
}
fprintf(f, "\n");
}
freeMem(summaryLine);
}
static void mafOrAxtClick2(struct sqlConnection *conn, struct sqlConnection *conn2, struct trackDb *tdb, char *axtOtherDb, char *fileName)
/* Display details for MAF or AXT tracks. */
{
hgBotDelay();
if (winEnd - winStart > 30000)
{
printf("Zoom so that window is 30,000 bases or less to see alignments and conservation statistics\n");
}
else
{
struct mafAli *mafList = NULL, *maf, *subList = NULL;
int aliIx = 0, realCount = 0;
char dbChrom[64];
char option[128];
char *capTrack;
struct consWiggle *consWig, *consWiggles;
struct hash *speciesOffHash = NULL;
char *speciesOrder = NULL;
char *speciesTarget = trackDbSetting(tdb, SPECIES_TARGET_VAR);
char buffer[1024];
int useTarg = FALSE;
int useIrowChains = FALSE;
safef(option, sizeof(option), "%s.%s", tdb->track, MAF_CHAIN_VAR);
if (cartCgiUsualBoolean(cart, option, FALSE) &&
trackDbSetting(tdb, "irows") != NULL)
useIrowChains = TRUE;
safef(buffer, sizeof(buffer), "%s.vis",tdb->track);
if (useIrowChains)
{
if (!cartVarExists(cart, buffer) && (speciesTarget != NULL))
useTarg = TRUE;
else
{
char *val;
val = cartUsualString(cart, buffer, "useCheck");
useTarg = sameString("useTarg",val);
}
}
if (sameString(tdb->type, "bigMaf"))
{
char *fileName = trackDbSetting(tdb, "bigDataUrl");
struct bbiFile *bbi = bigBedFileOpen(fileName);
mafList = bigMafLoadInRegion(bbi, seqName, winStart, winEnd);
}
else
mafList = mafOrAxtLoadInRegion2(conn,conn2, tdb, seqName, winStart, winEnd,
axtOtherDb, fileName);
safef(dbChrom, sizeof(dbChrom), "%s.%s", hubConnectSkipHubPrefix(database), seqName);
safef(option, sizeof(option), "%s.speciesOrder", tdb->track);
speciesOrder = cartUsualString(cart, option, NULL);
if (speciesOrder == NULL)
speciesOrder = trackDbSetting(tdb, "speciesOrder");
for (maf = mafList; maf != NULL; maf = maf->next)
{
int mcCount = 0;
struct mafComp *mc;
struct mafAli *subset;
struct mafComp *nextMc;
/* remove empty components and configured off components
* from MAF, and ignore
* the entire MAF if all components are empty
* (solely for gap annotation) */
if (!useTarg)
{
for (mc = maf->components->next; mc != NULL; mc = nextMc)
{
char buf[64];
char *organism;
mafSrcDb(mc->src, buf, sizeof buf);
organism = hOrganism(buf);
if (!organism)
organism = buf;
nextMc = mc->next;
safef(option, sizeof(option), "%s.%s", tdb->track, buf);
if (!cartUsualBoolean(cart, option, TRUE))
{
if (speciesOffHash == NULL)
speciesOffHash = newHash(4);
hashStoreName(speciesOffHash, organism);
}
if (!cartUsualBoolean(cart, option, TRUE))
slRemoveEl(&maf->components, mc);
else
mcCount++;
}
}
if (mcCount == 0)
continue;
if (speciesOrder)
{
int speciesCt;
char *species[2048];
struct mafComp **newOrder, *mcThis;
int i;
mcCount = 0;
speciesCt = chopLine(cloneString(speciesOrder), species);
newOrder = needMem((speciesCt + 1) * sizeof (struct mafComp *));
newOrder[mcCount++] = maf->components;
for (i = 0; i < speciesCt; i++)
{
if ((mcThis = mafMayFindCompSpecies(maf, species[i], '.')) == NULL)
continue;
newOrder[mcCount++] = mcThis;
}
maf->components = NULL;
for (i = 0; i < mcCount; i++)
{
newOrder[i]->next = 0;
slAddHead(&maf->components, newOrder[i]);
}
slReverse(&maf->components);
}
subset = mafSubsetE(maf, dbChrom, winStart, winEnd, TRUE);
if (subset != NULL)
{
/* Reformat MAF if needed so that sequence from current
* database is the first component and on the
* plus strand. */
mafMoveComponentToTop(subset, dbChrom);
if (subset->components->strand == '-')
mafFlipStrand(subset);
subset->score = mafScoreMultiz(subset);
slAddHead(&subList, subset);
++realCount;
}
}
slReverse(&subList);
mafAliFreeList(&mafList);
if (subList != NULL)
{
char *showVarName = "hgc.showMultiBase";
char *showVarVal = cartUsualString(cart, showVarName, "all");
boolean onlyDiff = sameWord(showVarVal, "diff");
#ifdef ADDEXONCAPITAL
char *codeVarName = "hgc.multiCapCoding";
char *codeVarVal = cartUsualString(cart, codeVarName, "coding");
boolean onlyCds = sameWord(codeVarVal, "coding");
#endif
/* add links for conservation score statistics */
consWiggles = wigMafWiggles(database, tdb);
int wigCount = slCount(consWiggles);
if (wigCount == 1)
{
conservationStatsLink(tdb, "Conservation score statistics", consWiggles->table);
}
else if (wigCount > 1)
{
/* multiple wiggles. List all that have been turned on with
* checkboxes */
/* Scan for cart variables -- do any exist, are any turned on ? */
boolean wigSet = FALSE;
boolean wigOn = FALSE;
for (consWig = consWiggles; consWig != NULL;
consWig = consWig->next)
{
char *wigVarSuffix = NULL;
(void)wigMafWiggleVar(tdb->track, consWig, &wigVarSuffix);
if (cartVarExistsAnyLevel(cart, tdb, FALSE, wigVarSuffix))
{
wigSet = TRUE;
if (cartBooleanClosestToHome(cart, tdb, FALSE, wigVarSuffix))
wigOn = TRUE;
}
}
/* If there are no cart vars, turn on the first (default) wig */
if (!wigSet)
{
char *prefix = tdb->track; // use when setting things to the cart
if (tdbIsContainerChild(tdb))
prefix = tdbGetContainer(tdb)->track;
cartSetBoolean(cart, wigMafWiggleVar(prefix, consWiggles, NULL), TRUE);
wigOn = TRUE;
}
if (wigOn)
{
boolean first = TRUE;
for (consWig = consWiggles; consWig != NULL;
consWig = consWig->next)
{
if (first)
{
printf("Conservation score statistics:");
first = FALSE;
}
char *wigVarSuffix = NULL;
(void)wigMafWiggleVar(tdb->track, consWig, &wigVarSuffix);
if (cartUsualBooleanClosestToHome(cart, tdb, FALSE, wigVarSuffix,FALSE))
{
printf(" ");
subChar(consWig->uiLabel, '_', ' ');
conservationStatsLink(tdb,
consWig->uiLabel, consWig->table);
}
}
}
}
puts("</P>\n");
/* no alignment to display when in visibilities where only wiggle is shown */
char *vis = cartOptionalString(cart, tdb->track);
if (vis)
{
enum trackVisibility tv = hTvFromStringNoAbort(vis);
if (tv == tvSquish || tv == tvDense)
return;
}
#ifdef ADDEXONCAPITAL
puts("<FORM ACTION=\"../cgi-bin/hgc\" NAME=\"gpForm\" METHOD=\"GET\">");
cartSaveSession(cart);
cgiContinueHiddenVar("g");
cgiContinueHiddenVar("c");
cgiContinueHiddenVar("i");
printf("Capitalize ");
cgiMakeDropListFull(codeVarName, codeAll, codeAll,
ArraySize(codeAll), codeVarVal, autoSubmit);
printf("exons based on ");
capTrack = genePredDropDown(cart, trackHash,
"gpForm", "hgc.multiCapTrack");
#endif
printf("show ");
cgiMakeDropListFull(showVarName, showAll, showAll,
ArraySize(showAll), showVarVal, autoSubmit);
printf("bases");
printf("<BR>\n");
printf("</FORM>\n");
#ifdef REVERSESTRAND
/* notify if bases are complemented (hgTracks is on reverse strand) */
if (cartCgiUsualBoolean(cart, COMPLEMENT_BASES_VAR, FALSE))
puts("<EM>Alignment displayed on reverse strand</EM><BR>");
#endif
puts("Place cursor over species for alignment detail. Click on 'B' to link to browser ");
puts("for aligned species, click on 'D' to get DNA for aligned species.<BR>");
printf("<TT><PRE>");
/* notify if species removed from alignment */
if (speciesOffHash)
{
char *species;
struct hashCookie hc = hashFirst(speciesOffHash);
puts("<B>Components not displayed:</B> ");
while ((species = hashNextName(&hc)) != NULL)
printf("%s ", species);
puts("<BR>");
}
for (maf = subList; maf != NULL; maf = maf->next)
{
mafLowerCase(maf);
#ifdef ADDEXONCAPITAL
if (capTrack != NULL)
capMafOnTrack(maf, capTrack, onlyCds);
#endif
printf("<B>Alignment block %d of %d in window, %d - %d, %d bps </B>\n",
++aliIx,realCount,maf->components->start + 1,
maf->components->start + maf->components->size, maf->components->size);
mafPrettyOut(stdout, maf, 70,onlyDiff, aliIx);
}
mafAliFreeList(&subList);
}
else
{
printf("No multiple alignment in browser window");
}
printf("</PRE></TT>");
}
}
static void hgSeqFeatureRegionOptions(struct cart *cart, boolean canDoUTR,
boolean canDoIntrons)
/* Print out HTML FORM entries for feature region options. */
{
char *exonStr = canDoIntrons ? " Exons" : "";
char *setting;
puts("\n<H3> Sequence Retrieval Region Options: </H3>\n");
if (canDoIntrons || canDoUTR)
{
cgiMakeCheckBox("hgSeq.promoter",
cartCgiUsualBoolean(cart, "hgSeq.promoter", FALSE));
puts("Promoter/Upstream by ");
setting = cartCgiUsualString(cart, "hgSeq.promoterSize", "1000");
cgiMakeTextVar("hgSeq.promoterSize", setting, 5);
puts("bases <BR>");
}
if (canDoUTR)
{
cgiMakeCheckBox("hgSeq.utrExon5",
cartCgiUsualBoolean(cart, "hgSeq.utrExon5", TRUE));
printf("5' UTR%s <BR>\n", exonStr);
}
if (canDoIntrons)
{
cgiMakeCheckBox("hgSeq.cdsExon",
cartCgiUsualBoolean(cart, "hgSeq.cdsExon", TRUE));
if (canDoUTR)
printf("CDS Exons <BR>\n");
else
printf("Blocks <BR>\n");
}
else if (canDoUTR)
{
cgiMakeCheckBox("hgSeq.cdsExon",
cartCgiUsualBoolean(cart, "hgSeq.cdsExon", TRUE));
printf("CDS <BR>\n");
}
else
{
cgiMakeHiddenVar("hgSeq.cdsExon", "1");
}
if (canDoUTR)
{
cgiMakeCheckBox("hgSeq.utrExon3",
cartCgiUsualBoolean(cart, "hgSeq.utrExon3", TRUE));
printf("3' UTR%s <BR>\n", exonStr);
}
if (canDoIntrons)
{
cgiMakeCheckBox("hgSeq.intron",
cartCgiUsualBoolean(cart, "hgSeq.intron", TRUE));
if (canDoUTR)
puts("Introns <BR>");
else
puts("Regions between blocks <BR>");
}
if (canDoIntrons || canDoUTR)
{
cgiMakeCheckBox("hgSeq.downstream",
cartCgiUsualBoolean(cart, "hgSeq.downstream", FALSE));
puts("Downstream by ");
setting = cartCgiUsualString(cart, "hgSeq.downstreamSize", "1000");
cgiMakeTextVar("hgSeq.downstreamSize", setting, 5);
puts("bases <BR>");
}
if (canDoIntrons || canDoUTR)
{
setting = cartCgiUsualString(cart, "hgSeq.granularity", "gene");
cgiMakeRadioButton("hgSeq.granularity", "gene",
sameString(setting, "gene"));
if (canDoUTR)
puts("One FASTA record per gene. <BR>");
else
puts("One FASTA record per item. <BR>");
cgiMakeRadioButton("hgSeq.granularity", "feature",
sameString(setting, "feature"));
if (canDoUTR)
puts("One FASTA record per region (exon, intron, etc.) with ");
else
puts("One FASTA record per region (block/between blocks) with ");
}
else
{
puts("Add ");
}
setting = cartCgiUsualString(cart, "hgSeq.padding5", "0");
cgiMakeTextVar("hgSeq.padding5", setting, 5);
puts("extra bases upstream (5') and ");
setting = cartCgiUsualString(cart, "hgSeq.padding3", "0");
cgiMakeTextVar("hgSeq.padding3", setting, 5);
puts("extra downstream (3') <BR>");
if (canDoIntrons && canDoUTR)
{
puts(" ");
cgiMakeCheckBox("hgSeq.splitCDSUTR",
cartCgiUsualBoolean(cart, "hgSeq.splitCDSUTR", FALSE));
puts("Split UTR and CDS parts of an exon into separate FASTA records");
}
puts("<BR>\n");
puts("Note: if a feature is close to the beginning or end of a chromosome \n"
"and upstream/downstream bases are added, they may be truncated \n"
"in order to avoid extending past the edge of the chromosome. <P>");
}
void doMiddle(struct cart *theCart)
/* Set up globals and make web page */
{
/* struct liftOverChain *chainList = NULL, *chain; */
char *userData;
/* char *dataFile; */
char *dataFormat;
char *organism;
char *db;
float minBlocks, minMatch;
boolean multiple, fudgeThick;
int minSizeQ, minSizeT;
boolean refreshOnly = FALSE;
/* char *err = NULL; */
struct liftOverChain *chainList = NULL, *choice;
cart = theCart;
if (cgiOptionalString(HGLFT_ERRORHELP_VAR))
{
puts("<PRE>");
puts(liftOverErrHelp());
//system("/usr/bin/cal");
puts("</PRE>");
return;
}
/* Get data to convert - from userData variable, or if
* that is empty from a file. */
if (cartOptionalString(cart, "SubmitFile"))
userData = cartOptionalString(cart, HGLFT_DATAFILE_VAR);
else
userData = cartOptionalString(cart, HGLFT_USERDATA_VAR);
dataFormat = cartCgiUsualString(cart, HGLFT_DATAFORMAT_VAR, DEFAULT_FORMAT);
cartWebStart(cart, NULL, "Lift Genome Annotations");
getDbAndGenome(cart, &db, &organism, oldVars);
chainList = liftOverChainListFiltered();
choice = defaultChoices(chainList, db);
if (choice == NULL)
errAbort("Sorry, no conversions available from this assembly\n");
minSizeQ = cartCgiUsualInt(cart, HGLFT_MINSIZEQ, choice->minSizeQ);
minSizeT = cartCgiUsualInt(cart, HGLFT_MINSIZET, choice->minSizeT);
minBlocks = cartCgiUsualDouble(cart, HGLFT_MINBLOCKS, choice->minBlocks);
minMatch = cartCgiUsualDouble(cart, HGLFT_MINMATCH, choice->minMatch);
fudgeThick = cartCgiUsualBoolean(cart, HGLFT_FUDGETHICK, (choice->fudgeThick[0]=='Y') ? TRUE : FALSE);
multiple = cartCgiUsualBoolean(cart, HGLFT_MULTIPLE, (choice->multiple[0]=='Y') ? TRUE : FALSE);
refreshOnly = cartCgiUsualInt(cart, HGLFT_REFRESHONLY_VAR, 0);
webMain(choice, dataFormat, multiple);
liftOverChainFreeList(&chainList);
if (!refreshOnly && userData != NULL && userData[0] != '\0')
{
struct hash *chainHash = newHash(0);
char *chainFile;
struct tempName oldTn, mappedTn, unmappedTn;
FILE *old, *mapped, *unmapped;
char *line;
int lineSize;
char *fromDb, *toDb;
int ct = 0, errCt = 0;
/* read in user data and save to file */
makeTempName(&oldTn, HGLFT, ".user");
old = mustOpen(oldTn.forCgi, "w");
fputs(userData, old);
fputs("\n", old); /* in case user doesn't end last line */
carefulClose(&old);
chmod(oldTn.forCgi, 0666);
/* setup output files -- one for converted lines, the other
* for lines that could not be mapped */
makeTempName(&mappedTn, HGLFT, ".bed");
makeTempName(&unmappedTn, HGLFT, ".err");
mapped = mustOpen(mappedTn.forCgi, "w");
chmod(mappedTn.forCgi, 0666);
unmapped = mustOpen(unmappedTn.forCgi, "w");
chmod(unmappedTn.forCgi, 0666);
fromDb = cgiString(HGLFT_FROMDB_VAR);
toDb = cgiString(HGLFT_TODB_VAR);
chainFile = liftOverChainFile(fromDb, toDb);
if (chainFile == NULL)
errAbort("ERROR: Can't convert from %s to %s: no chain file loaded",
fromDb, toDb);
readLiftOverMap(chainFile, chainHash);
if (sameString(dataFormat, WIGGLE_FORMAT))
/* TODO: implement Wiggle */
{}
else if (sameString(dataFormat, POSITION_FORMAT))
{
/* minSizeT here and in liftOverChain.c/h has been renamed minChainT in liftOver.c */
/* ignore multiple, it must be false when position is used */
ct = liftOverPositions(oldTn.forCgi, chainHash,
minMatch, minBlocks, 0, minSizeQ,
minSizeT, 0,
fudgeThick, mapped, unmapped, FALSE, NULL, &errCt);
}
else if (sameString(dataFormat, BED_FORMAT))
{
/* minSizeT here and in liftOverChain.c/h has been renamed minChainT in liftOver.c */
ct = liftOverBed(oldTn.forCgi, chainHash,
minMatch, minBlocks, 0, minSizeQ,
minSizeT, 0,
fudgeThick, mapped, unmapped, multiple, NULL, &errCt);
}
else
/* programming error */
errAbort("ERROR: Unsupported data format: %s\n", dataFormat);
webNewSection("Results");
if (ct)
{
/* some records succesfully converted */
cgiParagraph("");
printf("Successfully converted %d record", ct);
printf("%s: ", ct > 1 ? "s" : "");
printf("<A HREF=%s TARGET=_blank>View Conversions</A>\n", mappedTn.forCgi);
}
if (errCt)
{
/* some records not converted */
cgiParagraph("");
printf("Conversion failed on %d record", errCt);
printf("%s. ", errCt > 1 ? "s" : "");
printf("<A HREF=%s TARGET=_blank>Display failure file</A> \n",
unmappedTn.forCgi);
printf("<A HREF=\"../cgi-bin/hgLiftOver?%s=1\" TARGET=_blank>Explain failure messages</A>\n", HGLFT_ERRORHELP_VAR);
puts("<P>Failed input regions:\n");
struct lineFile *errFile = lineFileOpen(unmappedTn.forCgi, TRUE);
puts("<BLOCKQUOTE><PRE>\n");
while (lineFileNext(errFile, &line, &lineSize))
puts(line);
lineFileClose(&errFile);
puts("</PRE></BLOCKQUOTE>\n");
}
if (sameString(dataFormat, POSITION_FORMAT) && multiple)
{
puts("<BLOCKQUOTE><PRE>\n");
puts("Note: multiple checkbox ignored since it is not supported for position format.");
puts("</PRE></BLOCKQUOTE>\n");
}
carefulClose(&unmapped);
}
webDataFormats();
webDownloads();
cartWebEnd();
}
