static int feature_index_lua_add_feature_node(lua_State *L)
{
  GtFeatureIndex **fi;
  GtGenomeNode **gn;
  GtFeatureNode *fn;
  GtStr *seqid;
  GtError *err;
  bool has_seqid;
  gt_assert(L);
  fi = check_feature_index(L, 1);
  gn = check_genome_node(L, 2);
  fn = gt_feature_node_cast(*gn);
  luaL_argcheck(L, fn, 2, "not a feature node");
  seqid = gt_genome_node_get_seqid(*gn);
  luaL_argcheck(L, seqid, 2, "feature does not have a sequence id");
  err = gt_error_new();
  if (gt_feature_index_has_seqid(*fi, &has_seqid, gt_str_get(seqid), err))
    return gt_lua_error(L, err);
  gt_error_delete(err);
  luaL_argcheck(L, has_seqid, 2,
                "feature index does not contain corresponding sequence region");
  err = gt_error_new();
  if (gt_feature_index_add_feature_node(*fi, fn, err))
    return gt_lua_error(L, err);
  gt_error_delete(err);
  return 0;
}
static int feature_index_lua_get_features_for_range(lua_State *L)
{
  GtFeatureIndex **feature_index;
  const char *seqid;
  GtRange *range;
  GtError *err;
  bool has_seqid;
  GtArray *features;
  GT_UNUSED int had_err;

  feature_index = check_feature_index(L, 1);
  seqid = luaL_checkstring(L, 2);
  err = gt_error_new();
  if (gt_feature_index_has_seqid(*feature_index, &has_seqid, seqid, err))
    return gt_lua_error(L, err);
  gt_error_delete(err);
  luaL_argcheck(L, has_seqid, 2,
                "feature_index does not contain seqid");
  range = check_range(L, 3);
  features = gt_array_new(sizeof (GtGenomeNode*));
  err = gt_error_new();
  had_err = gt_feature_index_get_features_for_range(*feature_index, features,
                                                    seqid, range, err);
  if (had_err)
    return gt_lua_error(L, err);
  gt_error_delete(err);
  push_features_as_table(L, features);
  gt_array_delete(features);
  return 1;
}
Beispiel #3
0
static int feature_index_lua_get_range_for_seqid(lua_State *L)
{
  GtFeatureIndex **feature_index;
  const char *seqid;
  GtRange range;
  feature_index = check_feature_index(L, 1);
  seqid = luaL_checkstring(L, 2);
  luaL_argcheck(L, gt_feature_index_has_seqid(*feature_index, seqid), 2,
                "feature_index does not contain seqid");
  gt_feature_index_get_range_for_seqid(*feature_index, &range, seqid);
  return gt_lua_range_push(L, range);
}
static int feature_index_lua_has_seqid(lua_State *L)
{
  GtFeatureIndex **feature_index;
  bool has_seqid;
  const char *seqid;
  GtError *err;
  int had_err = 0;
  feature_index = check_feature_index(L, 1);
  seqid = luaL_checkstring(L, 2);
  err = gt_error_new();
  had_err = gt_feature_index_has_seqid(*feature_index, &has_seqid, seqid, err);
  if (had_err)
    return gt_lua_error(L, err);
  gt_error_delete(err);
  lua_pushboolean(L, has_seqid);
  return 1;
}
Beispiel #5
0
static int feature_index_lua_add_feature_node(lua_State *L)
{
  GtFeatureIndex **fi;
  GtGenomeNode **gn;
  GtFeatureNode *gf;
  GtStr *seqid;
  gt_assert(L);
  fi = check_feature_index(L, 1);
  gn = check_genome_node(L, 2);
  gf = gt_genome_node_cast(gt_feature_node_class(), *gn);
  luaL_argcheck(L, gf, 2, "not a feature node");
  seqid = gt_genome_node_get_seqid(*gn);
  luaL_argcheck(L, seqid, 2, "feature does not have a sequence id");
  luaL_argcheck(L, gt_feature_index_has_seqid(*fi, gt_str_get(seqid)), 2,
                "feature index does not contain corresponding sequence region");
  gt_feature_index_add_feature_node(*fi, gf);
  return 0;
}
Beispiel #6
0
static int feature_index_lua_get_features_for_range(lua_State *L)
{
  GtFeatureIndex **feature_index;
  const char *seqid;
  GtRange *range;
  GtArray *features;
  int had_err;
  feature_index = check_feature_index(L, 1);
  seqid = luaL_checkstring(L, 2);
  luaL_argcheck(L, gt_feature_index_has_seqid(*feature_index, seqid), 2,
                "feature_index does not contain seqid");
  range = check_range(L, 3);
  features = gt_array_new(sizeof (GtGenomeNode*));
  had_err = gt_feature_index_get_features_for_range(*feature_index, features,
                                                    seqid, range, NULL);
  gt_assert(!had_err); /* it was checked before that the feature_index contains
                          the given sequence id*/
  push_features_as_table(L, features);
  gt_array_delete(features);
  return 1;
}
static int feature_index_lua_get_range_for_seqid(lua_State *L)
{
  GtFeatureIndex **feature_index;
  const char *seqid;
  GtError *err;
  GtRange range;
  bool has_seqid;
  feature_index = check_feature_index(L, 1);
  seqid = luaL_checkstring(L, 2);
  err = gt_error_new();
  if (gt_feature_index_has_seqid(*feature_index, &has_seqid, seqid, err))
    return gt_lua_error(L, err);
  gt_error_delete(err);
  luaL_argcheck(L, has_seqid, 2,
                "feature_index does not contain seqid");
  err = gt_error_new();
  if (gt_feature_index_get_range_for_seqid(*feature_index, &range, seqid, err))
    return gt_lua_error(L, err);
  gt_error_delete(err);
  return gt_lua_range_push(L, range);
}
Beispiel #8
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static double gaeval_visitor_calculate_coverage(AgnGaevalVisitor *v,
                                                GtFeatureNode *genemodel,
                                                GtError *error)
{
  agn_assert(v && genemodel);

  GtStr *seqid = gt_genome_node_get_seqid((GtGenomeNode *)genemodel);
  GtRange mrna_range = gt_genome_node_get_range((GtGenomeNode *)genemodel);
  GtArray *overlapping = gt_array_new( sizeof(GtFeatureNode *) );
  bool hasseqid;
  gt_feature_index_has_seqid(v->alignments, &hasseqid, gt_str_get(seqid),error);
  if(hasseqid)
  {
    gt_feature_index_get_features_for_range(v->alignments, overlapping,
                                            gt_str_get(seqid), &mrna_range,
                                            error);
  }

  GtArray *exon_coverage = gt_array_new( sizeof(GtRange) );
  GtUword i;
  for(i = 0; i < gt_array_size(overlapping); i++)
  {
    GtFeatureNode *alignment = *(GtFeatureNode **)gt_array_get(overlapping, i);
    GtArray *covered_parts = gaeval_visitor_intersect((GtGenomeNode*)genemodel,
                                                      (GtGenomeNode*)alignment);
    if(covered_parts != NULL)
    {
      GtArray *temp = gaeval_visitor_union(exon_coverage, covered_parts);
      gt_array_delete(covered_parts);
      gt_array_delete(exon_coverage);
      exon_coverage = temp;
    }
  }
  double coverage = gaeval_visitor_coverage_resolve(genemodel, exon_coverage);
  gt_array_delete(exon_coverage);
  gt_array_delete(overlapping);

  return coverage;
}
Beispiel #9
0
static double gaeval_visitor_calculate_integrity(AgnGaevalVisitor *v,
                                                 GtFeatureNode *genemodel,
                                                 double coverage,
                                                 double *components,
                                                 GtError *error)
{
  agn_assert(v && genemodel);

  GtStr *seqid = gt_genome_node_get_seqid((GtGenomeNode *)genemodel);
  GtRange mrna_range = gt_genome_node_get_range((GtGenomeNode *)genemodel);
  GtArray *overlapping = gt_array_new( sizeof(GtFeatureNode *) );
  bool hasseqid;
  gt_feature_index_has_seqid(v->alignments, &hasseqid, gt_str_get(seqid),error);
  if(hasseqid)
  {
    gt_feature_index_get_features_for_range(v->alignments, overlapping,
                                            gt_str_get(seqid), &mrna_range,
                                            error);
  }

  GtArray *gaps = gt_array_new( sizeof(GtFeatureNode *) );
  while(gt_array_size(overlapping) > 0)
  {
    GtFeatureNode *alignment = *(GtFeatureNode **)gt_array_pop(overlapping);
    GtArray *agaps = agn_typecheck_select(alignment,
                                          gaeval_visitor_typecheck_gap);
    gt_array_add_array(gaps, agaps);
    gt_array_delete(agaps);
  }
  gt_array_delete(overlapping);

  GtUword utr5p_len = agn_mrna_5putr_length(genemodel);
  double utr5p_score = 0.0;
  if(utr5p_len >= v->params.exp_5putr_len)
    utr5p_score = 1.0;
  else
    utr5p_score = (double)utr5p_len / (double)v->params.exp_5putr_len;

  GtUword utr3p_len = agn_mrna_3putr_length(genemodel);
  double utr3p_score = 0.0;
  if(utr3p_len >= v->params.exp_3putr_len)
    utr3p_score = 1.0;
  else
    utr3p_score = (double)utr3p_len / (double)v->params.exp_3putr_len;

  GtArray *introns = agn_typecheck_select(genemodel, agn_typecheck_intron);
  GtUword exoncount = agn_typecheck_count(genemodel, agn_typecheck_exon);
  agn_assert(gt_array_size(introns) == exoncount - 1);
  double structure_score = 0.0;
  if(gt_array_size(introns) == 0)
  {
    GtUword cdslen = agn_mrna_cds_length(genemodel);
    if(cdslen >= v->params.exp_cds_len)
      structure_score = 1.0;
    else
      structure_score = (double)cdslen / (double)v->params.exp_cds_len;
  }
  else
  {
    structure_score = gaeval_visitor_introns_confirmed(introns, gaps);
  }
  gt_array_delete(gaps);
  gt_array_delete(introns);

  double integrity = (v->params.alpha   * structure_score) +
                     (v->params.beta    * coverage)        +
                     (v->params.gamma   * utr5p_score)     +
                     (v->params.epsilon * utr3p_score);
  if(components != NULL)
  {
    components[0] = structure_score;
    components[1] = coverage;
    components[2] = utr5p_score;
    components[3] = utr3p_score;
  }

  return integrity;
}
Beispiel #10
0
static int gt_sketch_page_runner(GT_UNUSED int argc,
                                 const char **argv,
                                 int parsed_args,
                                 void *tool_arguments,
                                 GtError *err)
{
  SketchPageArguments *arguments = tool_arguments;
  int had_err = 0;
  GtFeatureIndex *features = NULL;
  GtRange qry_range, sequence_region_range;
  GtStyle *sty = NULL;
  GtStr *prog, *gt_style_file;
  GtDiagram *d = NULL;
  GtLayout *l = NULL;
  GtBioseq *bioseq = NULL;
  GtCanvas *canvas = NULL;
  const char *seqid = NULL, *outfile;
  unsigned long start, height, num_pages = 0;
  double offsetpos, usable_height;
  cairo_surface_t *surf = NULL;
  cairo_t *cr = NULL;
  GtTextWidthCalculator *twc;
  gt_error_check(err);

  features = gt_feature_index_memory_new();

  if (cairo_version() < CAIRO_VERSION_ENCODE(1, 8, 6))
    gt_warning("Your cairo library (version %s) is older than version 1.8.6! "
               "These versions contain a bug which may result in "
               "corrupted PDF output!", cairo_version_string());

  /* get style */
  sty = gt_style_new(err);
  if (gt_str_length(arguments->stylefile) == 0)
  {
    prog = gt_str_new();
    gt_str_append_cstr_nt(prog, argv[0],
                          gt_cstr_length_up_to_char(argv[0], ' '));
    gt_style_file = gt_get_gtdata_path(gt_str_get(prog), err);
    gt_str_delete(prog);
    gt_str_append_cstr(gt_style_file, "/sketch/default.style");
  }
  else
  {
    gt_style_file = gt_str_ref(arguments->stylefile);
  }
  had_err = gt_style_load_file(sty, gt_str_get(gt_style_file), err);

  outfile = argv[parsed_args];
  if (!had_err)
  {
    /* get features */
    had_err = gt_feature_index_add_gff3file(features, argv[parsed_args+1], err);
     if (!had_err && gt_str_length(arguments->seqid) == 0) {
      seqid = gt_feature_index_get_first_seqid(features);
      if (seqid == NULL)
      {
        gt_error_set(err, "GFF input file must contain a sequence region!");
        had_err = -1;
      }
    }
    else if (!had_err
               && !gt_feature_index_has_seqid(features,
                                              gt_str_get(arguments->seqid)))
    {
      gt_error_set(err, "sequence region '%s' does not exist in GFF input file",
                   gt_str_get(arguments->seqid));
      had_err = -1;
    }
    else if (!had_err)
      seqid = gt_str_get(arguments->seqid);
  }

  /* set text */
  if (gt_str_length(arguments->text) == 0)
  {
    gt_str_delete(arguments->text);
    arguments->text = gt_str_new_cstr(argv[parsed_args+1]);
  }

  if (!had_err)
  {
    /* set display range */
    gt_feature_index_get_range_for_seqid(features, &sequence_region_range,
                                         seqid);
    qry_range.start = (arguments->range.start == GT_UNDEF_ULONG ?
                         sequence_region_range.start :
                         arguments->range.start);
    qry_range.end   = (arguments->range.end == GT_UNDEF_ULONG ?
                         sequence_region_range.end :
                         arguments->range.end);

    /* set output format */
    if (strcmp(gt_str_get(arguments->format), "pdf") == 0)
    {
      surf = cairo_pdf_surface_create(outfile,
                                      mm_to_pt(arguments->pwidth),
                                      mm_to_pt(arguments->pheight));
    }
    else if (strcmp(gt_str_get(arguments->format), "ps") == 0)
    {
      surf =  cairo_ps_surface_create(outfile,
                                      mm_to_pt(arguments->pwidth),
                                      mm_to_pt(arguments->pheight));
    }
    gt_log_log("created page with %.2f:%.2f dimensions\n",
                                                  mm_to_pt(arguments->pwidth),
                                                  mm_to_pt(arguments->pheight));

    offsetpos = TEXT_SPACER + arguments->theight + TEXT_SPACER;
    usable_height = mm_to_pt(arguments->pheight)
                              - arguments->theight
                              - arguments->theight
                              - 4*TEXT_SPACER;

    if (gt_str_length(arguments->seqfile) > 0) {
      bioseq = gt_bioseq_new(gt_str_get(arguments->seqfile), err);
    }

    cr = cairo_create(surf);
    cairo_set_font_size(cr, 8);
    twc = gt_text_width_calculator_cairo_new(cr, sty);
    for (start = qry_range.start; start <= qry_range.end;
         start += arguments->width)
    {
      GtRange single_range;
      GtCustomTrack *ct = NULL;
      const char *seq;
      single_range.start = start;
      single_range.end = start + arguments->width;

      if (had_err)
        break;

      d = gt_diagram_new(features, seqid, &single_range, sty, err);
      if (!d) {
        had_err = -1;
        break;
      }
      if (bioseq) {
        seq = gt_bioseq_get_sequence(bioseq, 0);
        ct = gt_custom_track_gc_content_new(seq,
                                      gt_bioseq_get_sequence_length(bioseq, 0),
                                      800, 70, 0.4, true);
        gt_diagram_add_custom_track(d, ct);
      }

      l = gt_layout_new_with_twc(d, mm_to_pt(arguments->width), sty, twc, err);
      had_err = gt_layout_get_height(l, &height, err);
      if (!had_err) {
        if (gt_double_smaller_double(usable_height - 10 - 2*TEXT_SPACER
              - arguments->theight, offsetpos + height))
        {
            draw_header(cr, gt_str_get(arguments->text), argv[parsed_args+1],
                        seqid, num_pages, mm_to_pt(arguments->pwidth),
                        mm_to_pt(arguments->pheight),
                        arguments->theight);
          cairo_show_page(cr);
          offsetpos = TEXT_SPACER + arguments->theight + TEXT_SPACER;
          num_pages++;
        }
        canvas = gt_canvas_cairo_context_new(sty,
                                             cr,
                                             offsetpos,
                                             mm_to_pt(arguments->pwidth),
                                             height,
                                             NULL,
                                             err);
        if (!canvas)
          had_err = -1;
        offsetpos += height;
        if (!had_err)
          had_err = gt_layout_sketch(l, canvas, err);
      }
      gt_canvas_delete(canvas);
      gt_layout_delete(l);
      gt_diagram_delete(d);
      if (ct)
        gt_custom_track_delete(ct);
    }
    draw_header(cr, gt_str_get(arguments->text), argv[parsed_args+1], seqid,
                num_pages, mm_to_pt(arguments->pwidth),
                mm_to_pt(arguments->pheight),
                arguments->theight);
    cairo_show_page(cr);
    num_pages++;
    gt_log_log("finished, should be %lu pages\n", num_pages);
    gt_text_width_calculator_delete(twc);
    cairo_destroy(cr);
    cairo_surface_flush(surf);
    cairo_surface_finish(surf);
    cairo_surface_destroy(surf);
    cairo_debug_reset_static_data();
    if (bioseq)
      gt_bioseq_delete(bioseq);
    gt_style_delete(sty);
    gt_str_delete(gt_style_file);
    gt_feature_index_delete(features);
  }
  return had_err;
}
/* to be called from implementing class! */
int gt_feature_index_unit_test(GtFeatureIndex *fi, GtError *err)
{
  int had_err = 0, i, rval;
  GtFeatureIndexTestShared sh;
  GtStrArray *seqids;
  GtStr *seqid;
  GtRange check_range;
  GtRegionNode *rn;
  bool has_seqid;
  gt_error_check(err);

  sh.mutex = gt_mutex_new();
  sh.nodes = gt_array_new(sizeof (GtFeatureNode*));
  sh.error_count = 0;
  sh.next_node_idx = 0;
  sh.fi = fi;
  sh.err = gt_error_new();

  /* create region */
  seqid = gt_str_new_cstr(GT_FI_TEST_SEQID);
  rn = (GtRegionNode*) gt_region_node_new(seqid, GT_FI_TEST_START,
                                          GT_FI_TEST_END);

  /* test seqid is not supposed to exist */
  gt_ensure(gt_feature_index_has_seqid(sh.fi, &has_seqid,
                                                 GT_FI_TEST_SEQID, err) == 0);
  gt_ensure(!has_seqid);

  /* add a sequence region directly and check if it has been added */
  rval = gt_feature_index_add_region_node(sh.fi, rn, err);
  gt_ensure(rval == 0);
  gt_genome_node_delete((GtGenomeNode*) rn);
  gt_ensure(gt_feature_index_has_seqid(sh.fi, &has_seqid,
                                                GT_FI_TEST_SEQID, err) == 0);
  gt_ensure(has_seqid);

  gt_feature_index_get_range_for_seqid(sh.fi, &check_range, GT_FI_TEST_SEQID,
                                       err);
  gt_ensure(check_range.start == GT_FI_TEST_START
                    && check_range.end == GT_FI_TEST_END);

  /* set up nodes to store */
  for (i=0;i<GT_FI_TEST_FEATURES_PER_THREAD*gt_jobs;i++) {
    GtUword start, end;
    GtFeatureNode *fn;
    start = random() % (GT_FI_TEST_END - GT_FI_TEST_FEATURE_WIDTH);
    end = start + random() % (GT_FI_TEST_FEATURE_WIDTH);
    fn = gt_feature_node_cast(gt_feature_node_new(seqid, "gene", start, end,
                                                  GT_STRAND_FORWARD));
    gt_array_add(sh.nodes, fn);
  }
  /* test parallel addition */
  gt_multithread(gt_feature_index_unit_test_add, &sh, err);
  seqids = gt_feature_index_get_seqids(fi, err);
  gt_ensure(seqids);
  gt_ensure(gt_feature_index_has_seqid(fi, &has_seqid,GT_FI_TEST_SEQID,
                                                err) == 0);
  gt_ensure(has_seqid);
  gt_ensure(gt_str_array_size(seqids) == 1);

  /* test parallel query */
  if (!had_err)
    gt_multithread(gt_feature_index_unit_test_query, &sh, err);
  gt_ensure(sh.error_count == 0);

  gt_mutex_delete(sh.mutex);
  gt_error_delete(sh.err);
  gt_str_array_delete(seqids);
  gt_array_delete(sh.nodes);
  gt_str_delete(seqid);
  return had_err;
}
Beispiel #12
0
static int gt_sketch_runner(int argc, const char **argv, int parsed_args,
                              void *tool_arguments, GT_UNUSED GtError *err)
{
  GtSketchArguments *arguments = tool_arguments;
  GtNodeStream *in_stream = NULL,
               *add_introns_stream = NULL,
               *gff3_out_stream = NULL,
               *feature_stream = NULL,
               *sort_stream = NULL,
               *last_stream;
  GtFeatureIndex *features = NULL;
  const char *file;
  char *seqid = NULL;
  GtRange qry_range, sequence_region_range;
  GtArray *results = NULL;
  GtStyle *sty = NULL;
  GtStr *prog, *defaultstylefile = NULL;
  GtDiagram *d = NULL;
  GtLayout *l = NULL;
  GtImageInfo* ii = NULL;
  GtCanvas *canvas = NULL;
  GtUword height;
  bool has_seqid;
  int had_err = 0;
  gt_error_check(err);
  gt_assert(arguments);

  prog = gt_str_new();
  gt_str_append_cstr_nt(prog, argv[0],
                        gt_cstr_length_up_to_char(argv[0], ' '));
  defaultstylefile = gt_get_gtdata_path(gt_str_get(prog), err);
  gt_str_delete(prog);
  if (!defaultstylefile)
    had_err = -1;
  if (!had_err) {
    gt_str_append_cstr(defaultstylefile, "/sketch/default.style");
  }

  file = argv[parsed_args];
  if (!had_err) {
    /* create feature index */
    features = gt_feature_index_memory_new();
    parsed_args++;

    /* create an input stream */
    if (strcmp(gt_str_get(arguments->input), "gff") == 0)
    {
      in_stream = gt_gff3_in_stream_new_unsorted(argc - parsed_args,
                                                 argv + parsed_args);
      if (arguments->verbose)
        gt_gff3_in_stream_show_progress_bar((GtGFF3InStream*) in_stream);
    } else if (strcmp(gt_str_get(arguments->input), "bed") == 0)
    {
      if (argc - parsed_args == 0)
        in_stream = gt_bed_in_stream_new(NULL);
      else
        in_stream = gt_bed_in_stream_new(argv[parsed_args]);
    } else if (strcmp(gt_str_get(arguments->input), "gtf") == 0)
    {
      if (argc - parsed_args == 0)
        in_stream = gt_gtf_in_stream_new(NULL);
      else
        in_stream = gt_gtf_in_stream_new(argv[parsed_args]);
    }
    last_stream = in_stream;

    /* create add introns stream if -addintrons was used */
    if (arguments->addintrons) {
      sort_stream = gt_sort_stream_new(last_stream);
      add_introns_stream = gt_add_introns_stream_new(sort_stream);
      last_stream = add_introns_stream;
    }

    /* create gff3 output stream if -pipe was used */
    if (arguments->pipe) {
      gff3_out_stream = gt_gff3_out_stream_new(last_stream, NULL);
      last_stream = gff3_out_stream;
    }

    /* create feature stream */
    feature_stream = gt_feature_stream_new(last_stream, features);

    /* pull the features through the stream and free them afterwards */
    had_err = gt_node_stream_pull(feature_stream, err);

    gt_node_stream_delete(feature_stream);
    gt_node_stream_delete(gff3_out_stream);
    gt_node_stream_delete(sort_stream);
    gt_node_stream_delete(add_introns_stream);
    gt_node_stream_delete(in_stream);
  }

  if (!had_err) {
    had_err = gt_feature_index_has_seqid(features,
                                         &has_seqid,
                                         gt_str_get(arguments->seqid),
                                         err);
  }

  /* if seqid is empty, take first one added to index */
  if (!had_err && strcmp(gt_str_get(arguments->seqid),"") == 0) {
    seqid = gt_feature_index_get_first_seqid(features, err);
    if (seqid == NULL) {
      gt_error_set(err, "GFF input file must contain a sequence region!");
      had_err = -1;
    }
  }
  else if (!had_err && !has_seqid) {
    gt_error_set(err, "sequence region '%s' does not exist in GFF input file",
                 gt_str_get(arguments->seqid));
    had_err = -1;
  }
  else if (!had_err)
    seqid = gt_str_get(arguments->seqid);

  results = gt_array_new(sizeof (GtGenomeNode*));
  if (!had_err) {
    had_err = gt_feature_index_get_range_for_seqid(features,
                                                   &sequence_region_range,
                                                   seqid,
                                                   err);
  }
  if (!had_err) {
    qry_range.start = (arguments->start == GT_UNDEF_UWORD ?
                         sequence_region_range.start :
                         arguments->start);
    qry_range.end   = (arguments->end == GT_UNDEF_UWORD ?
                         sequence_region_range.end :
                         arguments->end);
  }

  if (!had_err) {
    if (arguments->verbose)
      fprintf(stderr, "# of results: "GT_WU"\n", gt_array_size(results));

    /* find and load style file */
    if (!(sty = gt_style_new(err)))
      had_err = -1;
    if (gt_str_length(arguments->stylefile) == 0) {
      gt_str_append_str(arguments->stylefile, defaultstylefile);
    } else {
      if (!had_err && gt_file_exists(gt_str_get(arguments->stylefile))) {
        if (arguments->unsafe)
          gt_style_unsafe_mode(sty);
      }
      else
      {
        had_err = -1;
        gt_error_set(err, "style file '%s' does not exist!",
                          gt_str_get(arguments->stylefile));
      }
    }
    if (!had_err)
      had_err = gt_style_load_file(sty, gt_str_get(arguments->stylefile), err);
  }

  if (!had_err) {
    /* create and write image file */
    if (!(d = gt_diagram_new(features, seqid, &qry_range, sty, err)))
      had_err = -1;
    if (!had_err && arguments->flattenfiles)
      gt_diagram_set_track_selector_func(d, flattened_file_track_selector,
                                         NULL);
    if (had_err || !(l = gt_layout_new(d, arguments->width, sty, err)))
      had_err = -1;
    if (!had_err)
      had_err = gt_layout_get_height(l, &height, err);
    if (!had_err) {
      ii = gt_image_info_new();

      if (strcmp(gt_str_get(arguments->format),"pdf")==0) {
        canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_PDF,
                                          arguments->width,
                                          height, ii, err);
      }
      else if (strcmp(gt_str_get(arguments->format),"ps")==0) {
        canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_PS,
                                          arguments->width,
                                          height, ii, err);
      }
      else if (strcmp(gt_str_get(arguments->format),"svg")==0) {
        canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_SVG,
                                          arguments->width,
                                          height, ii, err);
      }
      else {
        canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_PNG,
                                          arguments->width,
                                          height, ii, err);
      }
      if (!canvas)
        had_err = -1;
      if (!had_err) {
        had_err = gt_layout_sketch(l, canvas, err);
      }
      if (!had_err) {
        if (arguments->showrecmaps) {
          GtUword i;
          const GtRecMap *rm;
          for (i = 0; i < gt_image_info_num_of_rec_maps(ii) ;i++) {
            char buf[BUFSIZ];
            rm = gt_image_info_get_rec_map(ii, i);
            (void) gt_rec_map_format_html_imagemap_coords(rm, buf, BUFSIZ);
            printf("%s, %s\n",
                   buf,
                   gt_feature_node_get_type(gt_rec_map_get_genome_feature(rm)));
          }
        }
        if (arguments->use_streams) {
          GtFile *outfile;
          GtStr *str = gt_str_new();
          gt_canvas_cairo_file_to_stream((GtCanvasCairoFile*) canvas, str);
          outfile = gt_file_open(GT_FILE_MODE_UNCOMPRESSED, file, "w+", err);
          if (outfile) {
            gt_file_xwrite(outfile, gt_str_get_mem(str), gt_str_length(str));
            gt_file_delete(outfile);
          } else {
            had_err = -1;
          }
          gt_str_delete(str);
        } else {
          had_err = gt_canvas_cairo_file_to_file((GtCanvasCairoFile*) canvas,
                                                 file,
                                                 err);
        }
      }
    }
  }

  /* free */
  gt_free(seqid);
  gt_canvas_delete(canvas);
  gt_layout_delete(l);
  gt_image_info_delete(ii);
  gt_style_delete(sty);
  gt_diagram_delete(d);
  gt_array_delete(results);
  gt_str_delete(defaultstylefile);
  gt_feature_index_delete(features);

  return had_err;
}