//Function: Constructor
//Description: Creates pointers to data structures contained by TwoPhotonGui to set up acquisition
// Sets up Vision modules
TwoPhotonThread::TwoPhotonThread(ScanEngine *scanEngi, AcqEngine *acqEngi, DataFile2P *data2Pi, zStepperDriver *zStepper, AomControl *aCtrl)
{
scanEng = scanEngi;
acqEng = acqEngi;
data2P = data2Pi;
zStep = *zStepper;
aomCtrl = aCtrl;
Point toolPos;
toolPos.x=850;
toolPos.y=20;
//temporary scaling
scaleMin1 = 0;
scaleMax1 = 32767;
scaleMin2 = 0;
scaleMax2 = 32767;
//Setup diplay and display tools using NIVision calls
image1 = imaqCreateImage(IMAQ_IMAGE_RGB, 0);
displayWinNum1 = 1;
imaqDisplayImage(image1, displayWinNum1, 1);
imaqSetWindowTitle(1, "PMT Images");
imaqSetWindowSize(1, 256, 256);
posWindow1.x = 850;
posWindow1.y = 300;
imaqMoveWindow(1, posWindow1);
imaqShowToolWindow (TRUE);
imaqMoveToolWindow(toolPos);
imaqSetCurrentTool (IMAQ_POLYLINE_TOOL);
image2 = imaqCreateImage(IMAQ_IMAGE_U8, 0);
displayWinNum2 = 2;
imaqDisplayImage(image2, displayWinNum2, 1);
imaqSetWindowTitle(2, "ROI Images");
imaqSetWindowSize(2, 256, 256);
posWindow2.x = 850;
posWindow2.y = 590;
imaqMoveWindow(2, posWindow2);
intScalingCoeff = 1.0;
bScaleCoeffCalc = false;
//Allocate memory for display arrays.
imageData1 = new RGBValue[acqEng->getnumValidXSamps() * acqEng->getnumValidYSamps()];
imageData2 = new unsigned char[acqEng->getWidth() * acqEng->getRepeats()];
bContinuous = false;
bLifetimeFov = false;
bLinescan = false;
}
int CVICALLBACK Thresholding (int panel, int control, int event,
void *callbackData, int eventData1, int eventData2)
{
int threshold = 0;
int histogram[256] = {0};
HistogramReport *report = NULL;
switch (event)
{
case EVENT_COMMIT:
GetCtrlVal (mypanel, MYPANEL_THRESHOLD_SLIDE, &threshold);
imaqThreshold (destimage, sourimage, threshold, 255, TRUE, 255);
imaqSetWindowTitle (1, "Picture after binaryzation");
imaqMoveWindow (1, imaqMakePoint (150, 260));
imaqDisplayImage (destimage, 1, TRUE);
report = imaqHistogram (destimage, 256, 0, 255, IMAQ_IMAGE_U8);
DeleteGraphPlot (mypanel, MYPANEL_MYGRAPH, -1, VAL_IMMEDIATE_DRAW);
PlotY (mypanel, MYPANEL_MYGRAPH, (*report).histogram, 256, VAL_UNSIGNED_INTEGER,
VAL_THIN_LINE, VAL_EMPTY_SQUARE, VAL_SOLID, 1, VAL_RED);
break;
}
return 0;
}
