pulsesequence()
{
double gt1 = getval("gt1"),
gzlvl1=getval("gzlvl1"),
gt2 = getval("gt2"),
gzlvl2=getval("gzlvl2"),
selpwrPS = getval("selpwrPS"),
selpwPS = getval("selpwPS"),
gzlvlPS = getval("gzlvlPS"),
droppts=getval("droppts"),
gstab = getval("gstab"),
espw180 = getval("espw180"),
essoftpw = getval("essoftpw"),
essoftpwr = getval("essoftpwr"),
essoftpwrf = getval("essoftpwrf");
char selshapePS[MAXSTR],
esshape[MAXSTR];
//synchronize gradients to srate for probetype='nano'
// Preserve gradient "area"
gt1 = syncGradTime("gt1","gzlvl1",1.0);
gzlvl1 = syncGradLvl("gt1","gzlvl1",1.0);
gt2 = syncGradTime("gt2","gzlvl2",1.0);
gzlvl2 = syncGradLvl("gt2","gzlvl2",1.0);
getstr("esshape",esshape);
getstr("selshapePS",selshapePS);
assign(ct,v17);
settable(t1,2,ph1);
settable(t2,8,ph2);
settable(t3,8,ph3);
settable(t4,4,ph4);
settable(t5,4,ph5);
settable(t6,4,ph6);
settable(t7,8,ph7);
getelem(t1,v17,v1);
getelem(t2,v17,v2);
getelem(t3,v17,v3);
getelem(t4,v17,v4);
getelem(t5,v17,v5);
getelem(t6,v17,v6);
getelem(t7,v17,v7);
assign(v7,oph);
/*assign(v1,v6);
add(oph,v18,oph);
add(oph,v19,oph);*/
/* BEGIN THE ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
delay(d1);
status(B);
obspower(tpwr);
rgpulse(pw, v1, rof1, rof2);
delay(d2/2.0);
zgradpulse(gzlvl1,gt1);
delay(gstab);
delay((0.25/sw1) - gt1 - gstab - 2*pw/PI - rof2 - 2*GRADIENT_DELAY - pw - rof1-essoftpw/2.0-2.0e-6);
obspower(essoftpwr+6); obspwrf(essoftpwrf);
shaped_pulse(esshape,essoftpw,v2,2.0e-6,2.0e-6);
obspower(tpwr); obspwrf(4095.0);
rgpulse(2.0*pw,v3,rof1,rof1);
zgradpulse(gzlvl1,gt1);
delay(gstab);
delay((0.25/sw1) - gt1 - gstab - 2*GRADIENT_DELAY - pw - rof1 - essoftpw/2.0-2.0e-6);
delay(gstab);
zgradpulse(gzlvl2,gt2);
delay(gstab);
obspower(essoftpwr+6); obspwrf(essoftpwrf);
shaped_pulse(esshape,essoftpw,v4,2.0e-6,2.0e-6);
obspower(selpwrPS); obspwrf(4095.0) ;
rgradient('z',gzlvlPS);
shaped_pulse(selshapePS,selpwPS,v5,rof1,rof1);
rgradient('z',0.0);
delay(gstab);
obspower(tpwr);
zgradpulse(gzlvl2,gt2);
delay(gstab - droppts/sw);
delay(d2/2.0);
obspower(tpwr); obspwrf(4095.0);
status(C);
}
pulsesequence()
{
double j1min = getval("j1min"),
j1max = getval("j1max"),
gzlvlE = getval("gzlvlE"),
gtE = getval("gtE"),
mult = getval("mult"),
pwx180 = getval("pwx180"),
pwxlvl180 = getval("pwxlvl180"),
pw180 = getval("pw180"),
pwx180r = getval("pwx180r"),
pwxlvl180r = getval("pwxlvl180r"),
tpwr180 = getval("tpwr180"),
EDratio = getval("EDratio"),
hsglvl = getval("hsglvl"),
hsgt = getval("hsgt"),
gstab = getval("gstab"),
dmfct = getval("dmfct"),
dpwrct = getval("dpwrct"),
tau,
tau1,
tau3,
taug,
bigT = getval("BigT");
char pwx180ad[MAXSTR],
pw180ad[MAXSTR],
dmct[MAXSTR],
bipflg[MAXSTR],
pwx180ref[MAXSTR],
dmmct[MAXSTR];
int icosel,
igcorr,
prgcycle = (int)(getval("prgcycle")+0.5),
phase1 = (int)(getval("phase")+0.5);
//synchronize gradients to srate for probetype='nano'
// Preserve gradient "area"
gtE = syncGradTime("gtE","gzlvlE",1.0);
gzlvlE = syncGradLvl("gtE","gzlvlE",1.0);
getstr("pwx180ad",pwx180ad);
getstr("pw180ad",pw180ad);
getstr("dmct",dmct);
getstr("pwx180ref", pwx180ref);
getstr("dmmct", dmmct);
getstr("bipflg",bipflg);
if (bipflg[0] == 'y')
{
tpwr180=getval("tnbippwr");
pw180=getval("tnbippw");
getstr("tnbipshp",pw180ad);
}
if (bipflg[1] == 'y')
{
pwxlvl180=getval("dnbippwr");
pwx180=getval("dnbippw");
getstr("dnbipshp",pwx180ad);
}
tau1 = 1/(2*(j1min + 0.07*(j1max - j1min)));
tau3 = 1/(2*(j1max - 0.07*(j1max - j1min)));
tau = 1 / (j1min+j1max);
taug = tau + getval("tauC");
icosel = 1;
igcorr = 2;
if (mult > 0.5)
igcorr = 1;
assign(ct,v17);
assign(zero,v18);
assign(zero,v19);
if (getflag("prgflg") && (satmode[0] == 'y') && (prgcycle > 1.5))
{
hlv(ct,v17);
mod2(ct,v18); dbl(v18,v18);
if (prgcycle > 2.5)
{
hlv(v17,v17);
hlv(ct,v19); mod2(v19,v19); dbl(v19,v19);
}
}
settable(t1, 4, ph1);
settable(t2, 8, ph2);
settable(t3, 16, ph3);
settable(t6, 2, ph6);
getelem(t1, v17, v1);
getelem(t3, v17, v3);
getelem(t2, v17, v2);
getelem(t6, v17, oph);
assign(zero, v4);
assign(zero,v6);
add(oph,v18,oph);
add(oph,v19,oph);
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v14);
/*
mod2(id2, v14);
dbl(v14,v14);
*/
add(v2, v14, v2);
add(v4, v14, v4);
add(oph, v14, oph);
if ((phase1 == 2) || (phase1 == 5))
icosel = -1;
status(A);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
if (satmode[0] == 'y')
{
if ((d1-satdly) > 0.02)
delay(d1-satdly);
else
delay(0.02);
if (getflag("slpsat"))
{
shaped_satpulse("relaxD",satdly,zero);
if (getflag("prgflg"))
shaped_purge(v6,zero,v18,v19);
}
else
{
satpulse(satdly,zero,rof1,rof1);
if (getflag("prgflg"))
purge(v6,zero,v18,v19);
}
}
else
delay(d1);
if (getflag("wet"))
wet4(zero,one);
obspower(tpwr);
decpower(pwxlvl);
if (getflag("nullflg"))
{
decpower(pwxlvl180);
rgpulse(0.5 * pw, zero, rof1, rof1);
obspower(tpwr180);
delay(tau/2.0);
simshaped_pulse(pw180ad,pwx180ad,pw180,pwx180,zero,zero,rof1,rof1);
delay(tau/2.0+POWER_DELAY+rof1);
delay(tau/2.0+POWER_DELAY+rof1);
simshaped_pulse(pw180ad,pwx180ad,pw180,pwx180,two,two,rof1,rof1);
obspower(tpwr);
delay(tau/2.0);
rgpulse(0.5 * pw, zero, rof1, rof1);
decpower(pwxlvl);
zgradpulse(hsglvl, 1.4*hsgt);
delay(1e-3);
}
status(B);
rgpulse(pw, zero, rof1, rof1);
if (getflag("dmct"))
{
decpower(dpwrct);
decprgon("garp1",1/dmfct,1.0);
/*
setstatus(DECch, FALSE, 'g', FALSE, dmfct);
*/
decunblank();
decon();
}
obspower(tpwr180);
delay(bigT/2 - d2/2 - tau - taug - pwx180r);
delay(pw180+WFG_START_DELAY+WFG_STOP_DELAY);
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
delay(bigT/2 - d2/2 - tau - taug - pwx180r);
delay(4*pw/PI+2*rof1+POWER_DELAY+4*pwx/PI+2*POWER_DELAY);
if (getflag("dmct"))
{
decoff();
decblank();
decprgoff();
/*
setstatus(DECch, FALSE, 'c', FALSE, dmf);
*/
decpower(pwxlvl);
}
if (mult > 0.5)
{
delay(tau-gtE-2*GRADIENT_DELAY-gstab);
zgradpulse(icosel*gzlvlE,gtE);
delay(gstab);
}
else
{
delay(tau);
}
decrgpulse(pwx,v2,rof1,rof1);
if (mult > 0.5)
{
decpower(pwxlvl180r);
delay(taug);
decshaped_pulse(pwx180ref, pwx180r, v4, rof1, rof1);
delay(d2/2);
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
delay(d2/2);
zgradpulse(icosel*gzlvlE,gtE);
delay(taug-gtE-2*GRADIENT_DELAY+(4*pwx/PI+2*rof1+2*POWER_DELAY-(WFG_START_DELAY+pw180+WFG_STOP_DELAY+2*rof1)));
decshaped_pulse(pwx180ref, pwx180r, v3, rof1, rof1);
}
else
{
decpower(pwxlvl180);
delay(taug/2+(pwx180r-pwx180)/2);
decshaped_pulse(pwx180ad, pwx180, v4, rof1, rof1);
delay(taug/2+(pwx180r-pwx180)/2.0-gtE-2*GRADIENT_DELAY-gstab);
zgradpulse(icosel*gzlvlE,gtE);
delay(gstab);
delay(d2/2);
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
delay(d2/2);
zgradpulse(icosel*gzlvlE,gtE);
delay(taug/2+(pwx180r-pwx180)/2.0+(4*pwx/PI+2*rof1+2*POWER_DELAY-WFG_START_DELAY-pw180-WFG_STOP_DELAY-2*rof1)-gtE-2*GRADIENT_DELAY);
decshaped_pulse(pwx180ad, pwx180, v3, rof1, rof1);
delay(taug/2+(pwx180r-pwx180)/2);
}
decpower(pwxlvl);
decrgpulse(pwx,v1,rof1,rof1);
obspower(tpwr);
delay(tau);
rgpulse(pw,one,rof1,rof2);
decrgpulse(pwx,zero,rof1,rof1);
obspower(tpwr180);
zgradpulse(igcorr*3*gzlvlE/(10*EDratio),gtE);
delay(tau3 - gtE - 2*GRADIENT_DELAY);
decrgpulse(pwx,zero,rof1,rof1);
zgradpulse(igcorr*7*gzlvlE/(10*EDratio),gtE);
delay(tau - gtE - 2*GRADIENT_DELAY);
decrgpulse(pwx,zero,rof1,rof1);
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
zgradpulse(igcorr*2*gzlvlE/EDratio,gtE);
delay(tau1 - gtE - 2*GRADIENT_DELAY - POWER_DELAY);
decpower(dpwr);
status(C);
delay(tau+tau3+3*pwx+6*rof1-tau1+WFG_START_DELAY+WFG_STOP_DELAY);
}
pulsesequence()
{
double mix,SSpwr,pwSS,hsdly,pwa,pwd,gt1,gzlvl1,gzlvl2;
char ptable[MAXSTR],SSshape[MAXSTR];
getstr("ptable",ptable); getstr("SSshape",SSshape);
mix=getval("mix"); hsdly=getval("hsdly");
loadtable(ptable);
pwa=getval("pwa"); pwd=getval("pwd");
gt1=getval("gt1"); gzlvl1=getval("gzlvl1"); gzlvl2=getval("gzlvl2");
SSpwr=getval("SSpwr"); pwSS=getval("pwSS");
initval(7.0,v2); /* set up 45 degree phase shift for first pulse */
obsstepsize(45.0);
getelem(t1,ct,v1); /* 1st pulse phase */
getelem(t3,ct,v3); /* read pulse phase */
getelem(t4,ct,v4); /* receiver phase */
if (phase1 > 1.5) incr(v1); /* hypercomplex phase shift */
assign(v4,oph);
/*HYPERCOMPLEX MODE USES REDFIELD TRICK TO MOVE AXIAL PEAKS TO EDGE */
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v6);
if ((phase1==1)||(phase1==2)) {add(v1,v6,v1); add(v4,v6,oph);}
assign(v3,v7); /* for steering pulses */
add(v3,one,v8);
if (pwa<0.0) { pwa = -pwa; add(v7,two,v7); }
if (pwd<0.0) { pwd = -pwd; add(v8,two,v8); }
/* CHECK VALIDITY OF PARAMETER RANGE */
if (dm[A] == 'y' || dm[B] == 'y')
{
printf(" dm must be 'nny' or 'nnn' ");
psg_abort(1);
}
if ((dm[C] == 'y' || dm2[C] == 'y') && at > 0.085)
{
printf(" check at time! Don't fry probe \n");
psg_abort(1);
}
if (dm2[A] == 'y' || dm2[B] == 'y')
{
printf(" dm2 must be 'nny' or 'nnn' ");
psg_abort(1);
}
if (dpwr > 50)
{
printf(" dpwr must be less than 49 \n");
psg_abort(1);
}
if (dpwr2 > 50)
{
printf(" dpwr2 must be less than 46 \n");
psg_abort(1);
}
/* BEGIN PULSE SEQUENCE */
status(A);
xmtrphase(v2);
txphase(v1);
obspower(tpwr);
hsdelay(d1);
status(B);
rgpulse(pw, v1, rof1, 1.0e-6);
xmtrphase(zero);
txphase(t2);
if (d2 > 0.001)
{
zgradpulse(gzlvl2,0.4*d2-SAPS_DELAY/2.0-2.0*GRADIENT_DELAY-(2.0*pw/PI));
delay(0.1*d2-rof1);
zgradpulse(-gzlvl2,0.4*d2-SAPS_DELAY/2.0-2.0*GRADIENT_DELAY-(2.0*pw/PI));
delay(0.1*d2-rof1);
}
else delay(d2);
rgpulse(pw, t2, rof1, 1.0e-6);
status(C);
obspower(SSpwr);
delay(hsdly);
zgradpulse(gzlvl1,gt1);
hsdelay(mix-hsdly-gt1);
shaped_pulse(SSshape,pwSS,t3,rof1,0.0);
obspower(SSpwr-12.0);
rgpulse(pwa,v7,2.0e-6,0.0); /* steering pulses */
rgpulse(pwd,v8,0.0e-6,0.0);
delay(rof2);
status(D);
}
pulsesequence()
{
double mixN = getval("mixN"),
gzlvlC = getval("gzlvlC"),
gtC = getval("gtC"),
gstab = getval("gstab"),
selpwrA = getval("selpwrA"),
selpwA = getval("selpwA"),
gzlvlA = getval("gzlvlA"),
gtA = getval("gtA"),
selpwrB = getval("selpwrB"),
selpwB = getval("selpwB"),
gzlvlB = getval("gzlvlB"),
gtB = getval("gtB"),
selfrq = getval("selfrq"),
zqfpw3 = getval("zqfpw3"),
zqfpwr3 = getval("zqfpwr3"),
gzlvlzq3 = getval("gzlvlzq3"),
mixNcorr,
sweeppw = getval("sweeppw"),
sweeppwr = getval("sweeppwr");
char sweepshp[MAXSTR],
selshapeA[MAXSTR],
selshapeB[MAXSTR],
zqfpat3[MAXSTR];
//synchronize gradients to srate for probetype='nano'
// Preserve gradient "area"
gtC = syncGradTime("gtC","gzlvlC",1.0);
gzlvlC = syncGradLvl("gtC","gzlvlC",1.0);
gtA = syncGradTime("gtA","gzlvlA",1.0);
gzlvlA = syncGradLvl("gtA","gzlvlA",1.0);
gtB = syncGradTime("gtB","gzlvlB",1.0);
gzlvlB = syncGradLvl("gtB","gzlvlB",1.0);
getstr("sweepshp",sweepshp);
getstr("selshapeA",selshapeA);
getstr("selshapeB",selshapeB);
getstr("zqfpat3",zqfpat3);
mixNcorr=0.0;
if (getflag("Gzqfilt"))
mixNcorr=getval("zqfpw3");
hlv(ct,v1); hlv(v1,v1); hlv(v1,v1); hlv(v1,v1); mod4(v1,v1);
mod4(ct,v2); add(v1,v2,v2);
hlv(ct,v3); hlv(v3,v3); mod4(v3,v3); add(v1,v3,v3); dbl(v3,v4);
dbl(v2,oph); add(oph,v4,oph); add(oph,v1,oph);
/* BEGIN THE ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
delay(5.0e-5);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
if (satmode[0] == 'y')
{
if ((d1-satdly) > 0.02)
delay(d1-satdly);
else
delay(0.02);
satpulse(satdly,zero,rof1,rof1);
}
else
delay(d1);
if (getflag("wet"))
wet4(zero,one);
status(B);
if (selfrq != tof)
obsoffset(selfrq);
rgpulse(pw, v1, rof1, rof1);
if (selfrq != tof)
obsoffset(selfrq);
zgradpulse(gzlvlA,gtA);
delay(gstab);
obspower(selpwrA);
shaped_pulse(selshapeA,selpwA,v2,rof1,rof1);
obspower(tpwr);
zgradpulse(gzlvlA,gtA);
delay(gstab);
if (selfrq != tof)
delay(2*OFFSET_DELAY);
zgradpulse(gzlvlB,gtB);
delay(gstab);
obspower(selpwrB);
shaped_pulse(selshapeB,selpwB,v3,rof1,rof1);
obspower(tpwr);
zgradpulse(gzlvlB,gtB);
delay(gstab);
if (selfrq != tof)
obsoffset(tof);
rgpulse(pw,v1,rof1,rof1);
obspower(sweeppwr);
delay(0.31*mixN);
zgradpulse(gzlvlC,gtC);
delay(gstab);
shaped_pulse(sweepshp,sweeppw,zero,rof1,rof1);
delay(gstab);
zgradpulse(-gzlvlC,gtC);
delay(0.49*mixN);
zgradpulse(-gzlvlC,2*gtC);
delay(gstab);
if (getflag("Gzqfilt"))
{
obspower(zqfpwr3);
rgradient('z',gzlvlzq3);
delay(100.0e-6);
shaped_pulse(zqfpat3,zqfpw3,zero,rof1,rof1);
delay(100.0e-6);
rgradient('z',0.0);
}
else
shaped_pulse(sweepshp,sweeppw,zero,rof1,rof1);
delay(gstab);
zgradpulse(gzlvlC,2*gtC);
delay(0.2*mixN);
obspower(tpwr);
rgpulse(pw,v1,rof1,rof2);
status(C);
}
pulsesequence()
{
/* DECLARE VARIABLES */
char fscuba[MAXSTR],f1180[MAXSTR],f2180[MAXSTR],fsat[MAXSTR],
shape[MAXSTR],sh_ad[MAXSTR],f3180[MAXSTR],
N_flg[MAXSTR],diag_supp[MAXSTR];
int phase,
phase2,
phase3,
t1_counter,
t2_counter,
t3_counter, icosel;
double hscuba, /* length of 1/2 scuba delay */
pwx2, /* PW90 for X-nuc */
tsatpwr, /* low power level for presat*/
dhpwr2, /* power level for X hard pulses */
jxh, /* coupling for XH */
tauxh, /* delay = 1/(2jxh) */
tau1, /* t1/2 H */
tau2, /* t2/2 N */
tau3, /* t3/2 N */
sw1, /* spectral width in 1H dimension */
sw2, /* spectral width in 15N dimension */
sw3, /* spectral width in 15N dimension */
MIX, /* Total Mixing time for noesy portion */
pw_sl, /* selective 2ms pulse on water */
tpwrsl, /* power level for pw_sl */
ppm,nst,pws,bw,ofs, /* used by Pbox */
pwN,pwNlvl,compH,compC,pwC,pwClvl,
d_ad, /* C high power for adiabatic pulses */
pwc_ad, /* C 90 pulse width */
zeta, /* Bax-Logan trick */
zeta1, /* Bax-Logan trick */
BigT,
BigT1,
gzlvl0,
gzlvl1,
gzlvl2,
gzlvl3,
gzlvl4,
gzlvl5,
gzlvl6,
gzlvl7,
gzlvl8,
gzlvl9,
gzlvl10,
gzlvl11,
gzlvl12,
gstab,
gt1,
gt2,
gt3,
gt4,
gt5,
gt6,
gt7,
gt8,
gt9,
gt10,
gt11,
gt12;
/* LOAD VARIABLES */
pwNlvl=getval("pwNlvl");
pwN=getval("pwN");
compC=getval("compC");
pwC=getval("pwC");
pwClvl=getval("pwClvl");
compH=getval("compH");
jxh = getval("jxh");
dhpwr2 = getval("dhpwr2");
pwx2 = getval("pwx2");
tsatpwr = getval("tsatpwr");
hscuba = getval("hscuba");
phase = (int) (getval("phase") + 0.5);
phase2 = (int) (getval("phase2") + 0.5);
phase3 = (int) (getval("phase3") + 0.5);
sw1 = getval("sw1");
sw2 = getval("sw2");
sw3 = getval("sw3");
MIX = getval("MIX");
pw_sl = getval("pw_sl");
tpwrsl = getval("tpwrsl");
pwc_ad = getval("pwc_ad");
d_ad = getval("d_ad");
ni = getval("ni");
BigT = getval("BigT");
BigT1 = getval("BigT1");
gstab = getval("gstab");
gzlvl0 = getval("gzlvl0");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl3 = getval("gzlvl3");
gzlvl4 = getval("gzlvl4");
gzlvl5 = getval("gzlvl5");
gzlvl6 = getval("gzlvl6");
gzlvl7 = getval("gzlvl7");
gzlvl8 = getval("gzlvl8");
gzlvl9 = getval("gzlvl9");
gzlvl10 = getval("gzlvl10");
gzlvl11 = getval("gzlvl11");
gzlvl12 = getval("gzlvl12");
gt1 = getval("gt1");
gt2 = getval("gt2");
gt3 = getval("gt3");
gt4 = getval("gt4");
gt5 = getval("gt5");
gt6 = getval("gt6");
gt7 = getval("gt7");
gt8 = getval("gt8");
gt9 = getval("gt9");
gt10 = getval("gt10");
gt11 = getval("gt11");
gt12 = getval("gt12");
getstr("fscuba",fscuba);
getstr("fsat",fsat);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("f3180",f3180);
getstr("N_flg",N_flg);
getstr("diag_supp",diag_supp);
getstr("sh_ad",sh_ad);
getstr("shape",shape);
if(d_ad > 62) {
printf("chirp power is too high \n");
psg_abort(1);
}
if(pwc_ad > 1.2e-3) {
printf("adiabatic pulse is too long; set < 0.5 ms\n");
psg_abort(1);
}
setautocal(); /* activate auto-calibration flags */
if (autocal[0] != 'n')
/* if autocal = 'y'(yes), 'q'(quiet), r(read), or 's'(semi) */
{
strcpy(shape,"H2Osel");
strcpy(sh_ad,"C13adiab");
if(FIRST_FID) /* call Pbox */
{
ppm = getval("dfrq"); ofs = 0.0; pws = 0.0005; /*0.5 ms pulse */
bw = 200.0*ppm; nst = 1000; /* nst - number of steps */
C13adiab = pbox_makeA("C13adiab", "sech", bw, pws, ofs, compC*pwC, pwClvl, nst);
H2Osel = pbox_Rsh("H2Osel", "sinc90", pw_sl, 0.0, compH*pw, tpwr);
ofs_check(H1ofs, C13ofs, N15ofs, H2ofs);
}
pw_sl = H2Osel.pw; tpwrsl = H2Osel.pwr-1.0; /* 1dB correction applied */
d_ad = C13adiab.pwr; pwc_ad = C13adiab.pw;
pwx2=pwN; dhpwr2=pwNlvl;
}
/* check validity of parameter range */
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y'))
{
printf("incorrect Dec1 decoupler flags! ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y'))
{
printf("incorrect Dec2 decoupler flags! ");
psg_abort(1);
}
if( tsatpwr > 8 )
{
printf("tsatpwr too large !!! ");
psg_abort(1);
}
if( dpwr > -16 )
{
printf("No C decoupling used in this expt. ");
psg_abort(1);
}
if( dpwr2 > -16 )
{
printf("No N decoupling used in this expt. ");
psg_abort(1);
}
if(gzlvl0 > 500) {
printf("gzlvl0_max is 500\n");
psg_abort(1);
}
if( gt1 > 3e-3 || gt2 > 3e-3 || gt3 > 3e-3 || gt4 > 3e-3
|| gt5 > 3e-3 || gt6 > 3e-3 || gt7 > 3e-3
|| gt8 > 3e-3 || gt9 > 3e-3 || gt10 > 3e-3 || gt11 > 3e-3
|| gt12 > 3e-3)
{
printf("gradients are on for too long !!! ");
psg_abort(1);
}
if(ix==1) {
if(f1180[A] != 'n' || f2180[A] !='y' || f3180[A] != 'y') {
printf("f1180 should be n, f2180 y, and f3180 should be y\n");
}
}
/* LOAD VARIABLES */
settable(t1, 4, phi1);
settable(t2, 1, phi2);
settable(t3, 1, phi3);
settable(t4, 1, phi4);
settable(t5, 1, phi5);
settable(t6, 1, phi6);
settable(t7, 1, phi7);
settable(t8, 1, phi8);
settable(t9, 2, phi9);
settable(t10, 4, rec);
/* INITIALIZE VARIABLES */
tauxh = 1/(4*jxh);
/* Phase incrementation for hypercomplex data */
if( phase == 2 ) {
tsadd(t2, 1, 4);
tsadd(t3, 1, 4);
}
if ( phase2 == 2 ) { /* Hypercomplex in t2 */
tsadd(t1, 1, 4);
}
if ( phase3 == 2 ) /* Hypercomplex in t3 */
{
tsadd(t6, 2, 4);
tsadd(t7, 2, 4);
tsadd(t8, 2, 4);
tsadd(t10, 2, 4);
icosel = -1;
}
else
icosel = 1;
/* calculate modifications to phases based on current t2/t3 values
to achieve States-TPPI acquisition */
if(ix==1)
d4_init = d4;
t3_counter = (int) ( (d4-d4_init)*sw3 + 0.5);
if(t3_counter %2) {
tsadd(t4,2,4);
tsadd(t5,2,4);
tsadd(t10,2,4);
}
if(ix==1)
d3_init = d3;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5);
if(t2_counter %2) {
tsadd(t1,2,4);
tsadd(t10,2,4);
}
if(ix==1)
d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5);
if(t1_counter %2) {
tsadd(t2,2,4);
tsadd(t3,2,4);
tsadd(t10,2,4);
}
/* set up so that get (-90,180) phase corrects in F1 if f1180 flag is y */
tau1 = d2;
if(f1180[A] == 'y') {
tau1 += 1/(2.0*sw1);
}
tau1 = tau1/2.0;
if(tau1 < 0.2e-6) tau1 = 0.2e-6;
/* set up so that get (-90,180) phase corrects in F2 if f2180 flag is y */
tau2 = d3;
if(f2180[A] == 'y')
tau2 += ( 1.0/(2.0*sw2) - (4.0/PI)*pwx2
- 2.0*(2.0*GRADIENT_DELAY + 50.0e-6 + 5.0e-6) );
if(f2180[A] == 'n')
tau2 = ( tau2 - (4.0/PI)*pwx2
- 2.0*(2.0*GRADIENT_DELAY + 50.0e-6 + 5.0e-6) );
tau2 = tau2/2.0;
if(ix==1)
if((tau2 + 5.0e-6 - 0.5*(WFG_START_DELAY + 4.0*pw + WFG_STOP_DELAY)
< 5.0e-6) && N_flg[A] == 'n')
printf("tau2 is negative; set f1180 to y\n");
/* set up so that get (-90,180) phase corrects in F3 if f3180 flag is y */
tau3 = d4;
if(f3180[A] == 'y') tau3 += ( 1.0/(2.0*sw3) );
tau3 = tau3/2.0;
if( tau3 < 0.2e-6) tau3 = 2.0e-7;
/* Now include Bax/Logan trick */
if(ni != 1) {
if(diag_supp[A] == 'n')
zeta = (tauxh - gt5 - 102.0e-6 + 2.0*pwx2 - 2.0e-6);
else
zeta = (1.0/(8.0*93.39) - gt5 - 102.0e-6 + 2.0*pwx2 - 2.0e-6);
zeta = zeta / ( (double) (ni-1) );
if(zeta < 0.0) {
printf("problem with zeta\n");
psg_abort(1);
}
}
else
zeta = 0.0;
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2 - d2_init)*sw1 + 0.5 );
zeta1 = zeta*( (double)t1_counter );
tau1 = tau1 - zeta1;
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tsatpwr); /* Set power for presaturation */
decpower(d_ad); /* Set decoupler1 power to d_ad */
dec2power(dhpwr2); /* Set decoupler2 power to dhpwr2 */
/* Presaturation Period */
if(fsat[0] == 'y')
{
txphase(zero);
rgpulse(d1,zero,2.0e-6,2.0e-6); /* presat */
obspower(tpwr); /* Set power for hard pulses */
if (fscuba[0] == 'y') /* Scuba pulse sequence */
{
hsdelay(hscuba);
rgpulse(pw,zero,1.0e-6,0.0); /* 90x180y90x */
rgpulse(2*pw,one,1.0e-6,0.0);
rgpulse(pw,zero,1.0e-6,0.0);
txphase(zero);
delay(hscuba);
}
}
else {
obspower(tpwr); /* Set power for hard pulses */
delay(d1);
}
status(B);
obsoffset(tof);
rcvroff();
delay(20.0e-6);
/* eliminate all magnetization originating from 15N */
dec2rgpulse(pwx2,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl1,gt1);
delay(gstab);
rgpulse(pw,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl2,gt2);
delay(2.0e-6);
delay(tauxh - gt2 - 4.0e-6); /* delay=1/4J(XH) */
sim3pulse(2*pw,0.0e-6,2*pwx2,zero,zero,zero,0.0,0.0);
delay(tauxh - gt2 - 202.0e-6); /* delay=1/4J(XH) */
txphase(one); dec2phase(t1);
delay(2.0e-6);
zgradpulse(gzlvl2,gt2);
delay(gstab);
rgpulse(pw,one,0.0,0.0);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,two,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
delay(2.0e-6);
zgradpulse(gzlvl3,gt3);
delay(gstab);
txphase(zero);
dec2rgpulse(pwx2,t1,0.0,0.0);
dec2phase(zero);
if(N_flg[A] == 'n')
{
if(tau2 + 5.0e-6 - 0.5*(WFG2_START_DELAY
+ pwc_ad + WFG2_STOP_DELAY) < 0.2e-6)
{
rgradient('z',gzlvl0); /* use rgradient since shaping takes more time */
delay(tau2 + 5.0e-6 - 0.5*(WFG_START_DELAY + 4.0*pw + WFG_STOP_DELAY));
rgradient('z',0.0);
delay(50.0e-6);
shaped_pulse("composite",4.0*pw,zero,0.0,0.0); /* 90x-180y-90x */
rgradient('z',-1.0*gzlvl0);
delay(tau2 + 5.0e-6 - 0.5*(WFG_START_DELAY + 4.0*pw + WFG_STOP_DELAY));
rgradient('z',0.0);
delay(50.0e-6);
}
else
{
rgradient('z',gzlvl0); /* use rgradient since shaping takes more time */
delay(tau2 + 5.0e-6 - 0.5*(WFG2_START_DELAY
+ pwc_ad + WFG2_STOP_DELAY));
rgradient('z',0.0);
delay(50.0e-6);
simshaped_pulse("composite",sh_ad,4.0*pw,pwc_ad,zero,zero,0.0,0.0);
rgradient('z',-1.0*gzlvl0); /* use rgradient since shaping takes more time */
delay(tau2 + 5.0e-6 - 0.5*(WFG2_START_DELAY
+ pwc_ad + WFG2_STOP_DELAY));
rgradient('z',0.0);
delay(50.0e-6);
}
} /* N_flg[A] == y */
else
sim3pulse(2.0*pw,0.0,2.0*pwx2,zero,zero,zero,4.0e-6,4.0e-6);
dec2rgpulse(pwx2,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl4,gt4);
delay(gstab);
if(diag_supp[A] == 'n') {
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,t3,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
rgpulse(pw,t2,2.0e-6,0.0);
txphase(t9);
delay(tau1 + tauxh + zeta1 - gt5 - 102.0e-6);
delay(2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(100.0e-6);
dec2rgpulse(2.0*pwx2,zero,0.0,0.0);
delay(tau1);
rgpulse(2.0*pw,t9,0.0,0.0); txphase(one);
delay(tauxh - zeta1 - gt5 - 102.0e-6 + 2.0*pwx2);
delay(2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(100.0e-6);
rgpulse(pw,one,0.0,0.0);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,one,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
delay(MIX - gt6 - gstab -2.0e-6);
delay(2.0e-6);
zgradpulse(gzlvl6,gt6);
delay(gt6);
delay(gstab);
}
else {
initval(1.0,v4);
obsstepsize(45.0);
xmtrphase(v4);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,t3,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
/*
xmtrphase(zero);
delay(2.0e-6);
initval(1.0,v4);
obsstepsize(45.0);
xmtrphase(v4);
*/
rgpulse(pw,t2,2.0e-6,0.0);
xmtrphase(zero);
txphase(t9);
delay(tau1 + 1.0/(8.0*93.39) + zeta1 - gt5 - 102.0e-6 - SAPS_DELAY);
delay(2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(100.0e-6);
dec2rgpulse(2.0*pwx2,zero,0.0,0.0);
delay(tau1);
rgpulse(2.0*pw,t9,0.0,0.0); txphase(one);
delay(1.0/(8.0*93.39) - zeta1 - gt5 - 102.0e-6 + 2.0*pwx2);
delay(2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(100.0e-6);
rgpulse(pw,one,0.0,0.0);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,one,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
delay(2.0e-6);
zgradpulse(gzlvl6,gt6/2.0);
delay(gstab);
delay(MIX - 1.5*gt6 - 2.0*(gstab+2.0e-6) - 2.0*pwx2);
dec2rgpulse(2.0*pwx2,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl6,gt6);
delay(gstab);
}
rgpulse(pw,two,2.0e-6,0.0);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,zero,2.0e-6,0.0);
delay(2.0e-6); txphase(zero);
obspower(tpwr);
/* shaped pulse */
delay(2.0e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
delay(tauxh
- POWER_DELAY - 2.0e-6 - WFG_START_DELAY
- pw_sl - WFG_STOP_DELAY - 2.0e-6 - POWER_DELAY
- gt7 - 4.0e-6); /* delay=1/4J(XH) */
sim3pulse(2*pw,0.0e-6,2*pwx2,zero,zero,zero,0.0,0.0);
delay(tauxh
- gt7 - gstab -2.0e-6
- POWER_DELAY - 2.0e-6 - WFG_START_DELAY
- pw_sl - WFG_STOP_DELAY - 2.0e-6 - POWER_DELAY);
delay(2.0e-6);
zgradpulse(gzlvl7,gt7);
delay(gt7);
delay(gstab);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,one,2.0e-6,0.0);
obspower(tpwr);
delay(2.0e-6); txphase(zero);
/* shaped pulse */
rgpulse(pw,one,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl8,gt8);
delay(gstab);
txphase(t6);
if(phase3 == 1)
dec2rgpulse(pwx2,t4,4.0e-6,0.0);
if(phase3 == 2)
dec2rgpulse(pwx2,t5,4.0e-6,0.0);
decphase(zero);
if(tau3 - 0.5*(WFG_START_DELAY
+ pwc_ad + WFG_STOP_DELAY)
< 0.2e-6) {
delay(tau3);
delay(tau3);
}
else {
delay(tau3 - 0.5*(WFG_START_DELAY
+ pwc_ad + WFG_STOP_DELAY));
decshaped_pulse(sh_ad,pwc_ad,zero,0.0,0.0);
delay(tau3 - 0.5*(WFG_START_DELAY
+ pwc_ad + WFG_STOP_DELAY));
}
delay(2.0e-6);
zgradpulse(-1.0*gzlvl11,gt11);
delay(100.0e-6);
delay(BigT - 4.0/PI*pwx2 + pw - 2.0*GRADIENT_DELAY - gt11
- 102.0e-6);
dec2rgpulse(2.0*pwx2,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl11,gt11);
delay(100.0e-6);
delay(BigT - 2.0*GRADIENT_DELAY - gt11
- 102.0e-6);
rgpulse(pw,t6,0.0,0.0);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,t7,2.0e-6,0.0);
delay(2.0e-6); txphase(zero);
obspower(tpwr);
/* shaped pulse */
delay(2.0e-6);
zgradpulse(gzlvl9,gt9);
delay(100.0e-6);
delay(tauxh
- POWER_DELAY - 2.0e-6 - WFG_START_DELAY
- pw_sl - WFG_STOP_DELAY - 2.0e-6 - POWER_DELAY
- gt9 - 102.0e-6);
sim3pulse(2.0*pw,0.0,2.0*pwx2,zero,zero,zero,0.0,0.0);
dec2phase(t8);
delay(2.0e-6);
zgradpulse(gzlvl9,gt9);
delay(100.0e-6);
delay(tauxh
- gt9 - 102.0e-6
- POWER_DELAY - 2.0e-6 - WFG_START_DELAY
- pw_sl - WFG_STOP_DELAY - 2.0e-6 - POWER_DELAY);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shape,pw_sl,zero,2.0e-6,0.0);
obspower(tpwr);
delay(2.0e-6); txphase(zero);
/* shaped pulse */
sim3pulse(pw,0.0,pwx2,zero,zero,t8,0.0,0.0);
dec2phase(zero);
delay(2.0e-6);
zgradpulse(gzlvl10,gt10);
delay(2.0e-6);
delay(tauxh - gt10 - 4.0e-6);
sim3pulse(2*pw,0.0e-6,2*pwx2,zero,zero,zero,2.0e-6,2.0e-6);
delay(2.0e-6);
zgradpulse(gzlvl10,gt10);
delay(100.0e-6);
delay(tauxh
- gt10 - 102.0e-6
+ 2.0/PI*pw - pwx2 + 0.5*(pwx2-pw));
dec2rgpulse(pwx2,zero,0.0,0.0);
dec2power(dpwr2); /* Very Important */
decpower(dpwr);
delay(BigT1 - 2.0*POWER_DELAY);
rgpulse(2.0*pw,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(-1.0*icosel*gzlvl12,gt12);
delay(50.0e-6);
delay(BigT1 - 2.0*GRADIENT_DELAY - 52.0e-6 - gt12);
/* acquire data */
status(C);
setreceiver(t10);
}
pulsesequence()
{
char
shname1[MAXSTR],
f1180[MAXSTR],
SE_flg[MAXSTR];
int icosel = 0,
t1_counter,
phase;
double d2_init=0.0,
pwS4,pwS5,pwS6,pwS7,
lambda = getval("lambda"),
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gzlvl3 = getval("gzlvl3"),
gzlvl5 = getval("gzlvl5"),
gt1 = getval("gt1"),
gt3 = getval("gt3"),
gt5 = getval("gt5"),
gstab = getval("gstab"),
shpw1,shlvl1=getval("shlvl1"),
pwClvl = getval("pwClvl"),
pwNlvl = getval("pwNlvl"),
pwN = getval("pwN"),
ni = getval("ni"),
d2 = getval("d2"),
tau1 = getval("tau1");
getstr("shname1", shname1);
getstr("SE_flg",SE_flg);
getstr("f1180",f1180);
phase = (int) (getval("phase") + 0.5);
settable(t3,2,phi3);
settable(t10,1,phi10);
settable(t12,2,phi12);
settable(t13,2,phi13);
/* INITIALIZE VARIABLES */
shpw1 = getval("shpw1");
pwS4 = h_shapedpw("eburp2",4.0,3.5,zero, 0.0, 0.0);
pwS5 = h_shapedpw("pc9f",4.0,3.5,zero, 2.0e-6, 0.0);
pwS6 = h_shapedpw("reburp",4.0,3.5,zero, 0.0, 0.0);
pwS7 = c_shapedpw2("isnob5",40.0,-125.0,"isnob5",40.0,0.0 , two, 0.0, 0.0);
if (SE_flg[0] =='y')
{
if (phase == 2) {tsadd(t10,2,4); icosel = +1;}
else icosel = -1;
}
else
{
if (phase == 2) {tsadd(t3,1,4); icosel=1;}
}
tau1 = d2;
if((f1180[A] == 'y') )
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
tau1 = tau1;
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{ tsadd(t3,2,4);tsadd(t12,2,4); tsadd(t13,2,4); }
status(A);
decpower(pwClvl);
dec2power(pwNlvl);
set_c13offset("co");
zgradpulse(gzlvl3, gt3);
delay(d1-gt3);
lk_hold();
rcvroff();
h_shapedpulse("pc9f",4.0,3.5,zero, 2.0e-6, 0.0);
delay(lambda-pwS5*0.5-pwS6*0.4);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, one, zero, zero, 0.0, 0.0);
delay(lambda-pwS5*0.5-pwS6*0.4);
h_shapedpulse("pc9f_",4.0,3.5,one, 0.0, 0.0);
obspower(shlvl1);
/**************************************************************************/
/** Sensitivity enhanced version **********************/
/**************************************************************************/
if (SE_flg[0] == 'y')
{
shaped_pulse(shname1,shpw1,zero,0.0,0.0);
dec2rgpulse(pwN,t3,0.0,0.0);
delay(tau1*0.5);
shaped_pulse(shname1,shpw1,two,0.0,0.0);
c_shapedpulse2("isnob5",40.0,-125.0,"isnob5",40.0,0.0 , two, 0.0, 0.0);
delay(tau1*0.5);
zgradpulse(-gzlvl1, gt1);
delay(pwS4-shpw1-pwS7-gt1);
dec2rgpulse(pwN*2.0,zero,0.0,0.0);
h_shapedpulse("eburp2",4.0,3.5,zero, 2.0e-6, 0.0);
dec2rgpulse(pwN, t10, 0.0, 0.0);
}
/**************************************************************************/
/** standard INEPT-based version **********************/
/**************************************************************************/
else
{
if (ni < 1.0)
{
dec2rgpulse(pwN,t3,0.0,0.0);
dec2rgpulse(pwN*2.0, zero, 0.0, 0.0);
dec2rgpulse(pwN, zero, 0.0, 0.0);
}
else
{
if (tau1 < shpw1)
{
shaped_pulse(shname1,shpw1,two,0.0,0.0);
shaped_pulse(shname1,shpw1,zero,0.0,0.0);
dec2rgpulse(pwN,t3,0.0,0.0);
delay(tau1);
dec2rgpulse(pwN*3.0, zero, 0.0, 0.0);
}
else
{
if (tau1*0.5 < (pwS7+shpw1*0.5))
{
shaped_pulse(shname1,shpw1,two,0.0,0.0);
dec2rgpulse(pwN,t3,0.0,0.0);
delay(tau1*0.5-shpw1*0.5);
shaped_pulse(shname1,shpw1,zero,0.0,0.0);
delay(tau1*0.5-shpw1*0.5);
dec2rgpulse(pwN*3.0, zero, 0.0, 0.0);
}
else
{
shaped_pulse(shname1,shpw1,two,0.0,0.0);
dec2rgpulse(pwN,t3,0.0,0.0);
delay(tau1*0.5-shpw1*0.5);
shaped_pulse(shname1,shpw1,zero,0.0,0.0);
c_shapedpulse2("isnob5",40.0,-125.0,"isnob5",40.0,0.0 , two, 0.0, 0.0);
delay(tau1*0.5-shpw1*0.5-pwS7);
dec2rgpulse(pwN*3.0, zero, 0.0, 0.0);
}
}
}
}
/**************************************************************************/
if (SE_flg[0] == 'y')
{
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5);
dec2rgpulse(pwN, one, 0.0, 0.0);
h_shapedpulse("eburp2_",4.0,3.5,one, 0.0, 0.0);
txphase(zero);
dec2phase(zero);
delay(lambda-pwS4*0.5-pwS6*0.4);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
dec2phase(t10);
delay(lambda-pwS4*0.5-pwS6*0.4);
h_shapedpulse("eburp2",4.0,3.5,zero, 0.0, 0.0);
delay((gt1/10.0) + 1.0e-4 +gstab + 2.0*GRADIENT_DELAY + POWER_DELAY);
h_shapedpulse("reburp",4.0,3.5,zero, 0.0, 0.0);
zgradpulse(icosel*gzlvl2, gt1/10.0); /* 2.0*GRADIENT_DELAY */
delay(gstab);
}
else
{
h_shapedpulse("eburp2",4.0,3.5,zero, 2.0e-6, 0.0);
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5-POWER_DELAY-1.0e-4);
}
dec2power(dpwr2); /* POWER_DELAY */
lk_sample();
if (SE_flg[0] == 'y')
setreceiver(t13);
else
setreceiver(t12);
statusdelay(C,1.0e-4 );
}
pulsesequence()
{
char f1180[MAXSTR],
f2180[MAXSTR],
mag_flg[MAXSTR],
flg_3919[MAXSTR],
ref_flg[MAXSTR];
int phase, ni2,
t1_counter,
t2_counter;
double gzcal = getval("gzcal"),
factor = 0.08, /* used for 3-9-19 water gate */
tau_3919 = getval("tau_3919"),
flipphase = getval("flipphase"),
tau1, /* t1 delay */
tau2, /* t2 delay */
taua, /* 1/4JNH ~ 2.3 ms */
taub, /* 1/4JNH ~ 2.3 ms */
bigT, /* ~ 19 ms */
pwNlvl,
pwN,
gt1,
gt2,
gt3,
gt4,
gt5,
gt6,
gt7,
gt8,
gzlvl1,
gzlvl2,
gzlvl3,
gzlvl4,
gzlvl5,
gzlvl6,
gzlvl7,
gzlvl8,
compH = getval("compH"), /* adjustment for C13 amplifier compression */
pwHs = getval("pwHs"), /* H1 90 degree pulse length at tpwrs */
tpwrsf = getval("tpwrsf"), /* fine power adjustment for flipback pulse */
tpwrs; /* power for the pwHs ("H2Osinc") pulse */
/* LOAD VARIABLES */
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("flg_3919", flg_3919);
getstr("mag_flg", mag_flg);
getstr("ref_flg", ref_flg);
taua = getval("taua");
taub = getval("taub");
bigT = getval("bigT");
tpwr = getval("tpwr");
pwNlvl = getval("pwNlvl");
pwN = getval("pwN");
dpwr = getval("dpwr");
dpwr2 = getval("dpwr2");
phase = (int)( getval("phase") + 0.5);
phase2 = (int)( getval("phase2") + 0.5);
sw1 = getval("sw1");
sw2 = getval("sw2");
ni = getval("ni");
ni2 = getval("ni2");
gt1 = getval("gt1");
gt2 = getval("gt2");
gt3 = getval("gt3");
gt4 = getval("gt4");
gt5 = getval("gt5");
gt6 = getval("gt6");
gt7 = getval("gt7");
gt8 = getval("gt8");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl3 = getval("gzlvl3");
gzlvl4 = getval("gzlvl4");
gzlvl5 = getval("gzlvl5");
gzlvl6 = getval("gzlvl6");
gzlvl7 = getval("gzlvl7");
gzlvl8 = getval("gzlvl8");
/* LOAD PHASE TABLE */
settable(t1,4,phi1);
settable(t2,2,phi2);
settable(t3,8,phi3);
settable(t4,16, phi4);
if (ref_flg[A] == 'y')
{
settable(t10,8,ref);
}
else
{
settable(t10,8,rec);
}
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /*needs 1.69 times more*/
tpwrs = (int) (tpwrs); /*power than a square pulse */
/* CHECK VALIDITY OF PARAMETER RANGES */
if (ref_flg[A] == 'y' && ni > 1)
{
printf(" Incorrect setting of ni and ref_flg.\n");
printf(" Please choose either ni=1 or ref_flg=n.\n");
psg_abort(1);
}
if (ref_flg[A] == 'y' && dps_flag)
{
printf(" Please use phase2 and ni2 for 2D reference spectrum\n");
if (ni2/sw2 > 2.0*(2.0*bigT - gt5 - 200.0e-6))
{
printf("ni2 is too big, should be < %f\n", 2.0*sw2*(2.0*bigT-gt5-200.0e-6));
psg_abort(1);
}
}
if ((ni2/sw2 > 2.0*(bigT - gt5 - 200.0e-6)) && (ref_flg[A] !='y'))
{
printf(" ni2 is too big, should be < %f\n", 2.0*sw2*(bigT-gt6-200.0e-6));
psg_abort(1);
}
if(dpwr2 > 50)
{
printf("don't fry the probe, dpwr2 is too large! ");
psg_abort(1);
}
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y'))
{
printf("incorrect dec1 decoupler flags! should be 'nnn' ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' ))
{
printf("incorrect dec2 decoupler flags! Should be 'nny' ");
psg_abort(1);
}
/* Phase incrementation for hypercomplex 2D data */
if (phase == 2)
{
tsadd(t1,1,4);
}
if (phase2 == 2)
{
tsadd(t2,1,4);
}
/* Set up f1180 half_dwell time (1/sw1)/2.0 */
tau1 = d2 - (4.0*pw/PI + 2.0*pwN);
if(f1180[A] == 'y')
{
tau1 += (1.0/(2.0*sw1));
}
if(tau1 < 0.2e-6) tau1 = 0.0;
tau1 = tau1/2.0;
/* Set up f2180 half dwell time (1/sw2)/2.0 */
tau2 = d3;
if(f2180[A] == 'y')
{
tau2 += (1.0/(2.0*sw2));
}
if(tau2 < 0.2e-6) tau2 = 0.0;
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2 ;
t1_counter = (int)((d2-d2_init)*sw1 + 0.5);
if(t1_counter % 2)
{
tsadd(t1,2,4);
tsadd(t10,2,4);
}
if( ix == 1) d3_init = d3 ;
t2_counter = (int)((d3-d3_init)*sw2 + 0.5);
if(t2_counter % 2)
{
tsadd(t2,2,4);
tsadd(t10,2,4);
}
if (flipphase < -0.01) flipphase = flipphase + 360.0;
initval(flipphase, v10);
obsstepsize(0.25);
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
dec2power(pwNlvl);
txphase(zero);
dec2phase(zero);
delay(d1);
if(gt1 > 0.2e-6)
{
dec2rgpulse(pwN, zero, 0.2e-6, 0.0);
delay(2.0e-6);
zgradpulse(gzlvl1, gt1);
delay(0.001);
}
rcvroff();
status(B);
rgpulse(pw, zero,rof1, 0.0);
delay(2.0e-6);
if (gt2 > 0.2e-6)
zgradpulse(gzlvl2,gt2);
delay(taua - gt2 - 2.0e-6);
sim3pulse(2.0*pw,(double)0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
delay(taua - gt2 - 400.0e-6);
if (gt2 > 0.2e-6)
{
zgradpulse(gzlvl2,gt2);
}
txphase(one);
dec2phase(t2);
delay(400.0e-6);
rgpulse(pw, one, 0.0, 0.0);
if (gt3 > 0.2e-6)
{
delay(2.0e-6);
zgradpulse(gzlvl3, gt3);
delay(200.0e-6);
}
txphase(zero);
dec2rgpulse(pwN, t2, 0.0, 0.0);
if (ref_flg[A] == 'y')
{
delay(tau2);
rgpulse(2.0*pw, zero, 0.0, 0.0);
dec2phase(t3);
if (gt5 > 0.2e-6)
{
delay(2.0*bigT - gt5 - 2.0*pw - 1.0e-3);
zgradpulse(gzlvl5, gt5);
delay(1.0e-3);
dec2rgpulse(2.0*pwN, t3, 0.0, 0.0);
delay(2.0e-6);
zgradpulse(gzlvl5, gt5);
delay(2.0*bigT - tau2 - gt5 - 2.0e-6);
}
else
{
delay(2.0*bigT - 2.0*pw);
dec2rgpulse(2.0*pwN, t3, 0.0, 0.0);
delay(2.0*bigT - tau2);
}
}
else
{
dec2phase(zero);
if (gt4 > 0.2e-6)
{
delay(2.0e-6);
zgradpulse(gzlvl4, gt4);
delay(bigT - gt4 - 2.0e-6);
sim3pulse(2.0*pw,(double)0.0, 2.0*pwN, zero,zero, zero, 0.0, 0.0);
delay(2.0e-6);
zgradpulse(gzlvl4, gt4);
delay(1.0e-3 - 2.0e-6);
}
else
{
delay(bigT);
sim3pulse(2.0*pw,(double)0.0, 2.0*pwN, zero,zero, zero, 0.0, 0.0);
delay(1.0e-3);
gt4 = 0.0;
}
zgradpulse(gzlvl5, gt5);
txphase(t1);
delay(bigT - gt4 - gt5 - 1.0e-3 - 2.0*GRADIENT_DELAY);
rgpulse(pw, t1, 0.0, 0.0);
delay(tau1);
dec2rgpulse(2.0*pwN, t3, 0.0, 0.0);
txphase(zero);
delay(tau1);
rgpulse(pw, zero, 0.0, 0.0);
delay(2.0e-6);
zgradpulse(gzlvl5, gt5);
dec2phase(t4);
if (gt6 > 0.2e-6)
{
delay(tau2 + 100.0e-6);
zgradpulse(gzlvl6, gt6);
delay(bigT - gt5 - gt6 - 100.0e-6 - 2.0*GRADIENT_DELAY);
sim3pulse(2.0*pw,(double)0.0, 2.0*pwN, zero,zero, t4, 0.0, 0.0);
delay(2.0e-6);
dec2phase(zero);
zgradpulse(gzlvl6, gt6);
delay(bigT - tau2 - gt6 - 2.0e-6);
}
else
{
delay(bigT + tau2 - gt5 - 2.0*GRADIENT_DELAY);
sim3pulse(2.0*pw,(double)0.0, 2.0*pwN, zero,zero, t4, 0.0, 0.0);
dec2phase(zero);
delay(bigT - tau2);
}
}
if (gt7 > 0.2e-6)
{
dec2rgpulse(pwN, zero, 0.0,2.0e-6);
zgradpulse(gzlvl7, gt7);
txphase(zero);
delay(200.0e-6);
if (pwHs > 0.2e-6)
{
xmtrphase(v10);
if (tpwrsf<4095.0) {obspower(tpwrs+6.0); obspwrf(tpwrsf);}
else obspower(tpwrs);
txphase(two);
shaped_pulse("H2Osinc", pwHs, two, 2.0e-6, 0.0);
xmtrphase(zero);
obspower(tpwr); obspwrf(4095.0);
}
rgpulse(pw, zero, 2.0e-6, 0.0);
}
else
{
sim3pulse(pw,(double)0.0, pwN, zero,zero, zero, 0.0, 0.0);
}
delay(2.0e-6);
if(mag_flg[A] == 'y')
{
magradpulse(gzcal*gzlvl8, gt8);
}
else
{
zgradpulse(gzlvl8, gt8);
}
if (flg_3919[A] == 'y')
{
delay(taub - 31.0*factor*pw - 2.5*tau_3919 - gt8 - 2.0e-6);
rgpulse(pw*factor*3.0, two, 0.0, 0.0);
delay(tau_3919);
rgpulse(pw*factor*9.0, two, 0.0, 0.0);
delay(tau_3919);
rgpulse(pw*factor*19.0, two, 0.0, 0.0);
delay(tau_3919/2.0 - pwN);
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
delay(tau_3919/2.0 - pwN);
rgpulse(pw*factor*19.0, zero, 0.0, 0.0);
delay(tau_3919);
rgpulse(pw*factor*9.0, zero, 0.0, 0.0);
delay(tau_3919);
rgpulse(pw*factor*3.0, zero, 0.0, 0.0);
delay(taub - 31.0*factor*pw - 2.5*tau_3919 - gt8 - POWER_DELAY - 402.0e-6);
}
else
{
if (tpwrsf<4095.0) {obspower(tpwrs+6.0); obspwrf(tpwrsf);}
else obspower(tpwrs);
txphase(two);
xmtrphase(v10);
delay(taub - pwHs - gt8 - 2.0*POWER_DELAY - 2.0e-6);
shaped_pulse("H2Osinc", pwHs, two, 0.0, 0.0);
obspower(tpwr); obspwrf(4095.0);
xmtrphase(zero);
txphase(zero);
sim3pulse(2.0*pw, (double)0.0, 2.0*pwN, zero, zero, zero, 2.0e-6, 0.0);
if (tpwrsf<4095.0) {obspower(tpwrs+6.0); obspwrf(tpwrsf);}
else obspower(tpwrs);
txphase(two);
xmtrphase(v10);
shaped_pulse("H2Osinc", pwHs, two, 2.0e-6, 0.0);
xmtrphase(zero);
obspower(tpwr); obspwrf(4095.0);
dec2power(dpwr2);
delay(taub - pwHs - gt8 - 3.0*POWER_DELAY - 402.0e-6);
}
dec2power(dpwr2);
if(mag_flg[A] == 'y')
{
magradpulse(gzcal*gzlvl8, gt8);
}
else
{
zgradpulse(gzlvl8, gt8);
}
delay(400.0e-6);
status(C);
setreceiver(t10);
rcvron();
}
void pulsesequence()
{
double slpwr,
slpw,
mix,
hsglvl,
hsgt,
gzlvlz,
gtz,
zfpw,
zfpwr,
cycles;
int iphase;
char sspul[MAXSTR],
composit[MAXSTR],
compshape[MAXSTR],
zfilt[MAXSTR],
zfshp[MAXSTR];
/* LOAD AND INITIALIZE PARAMETERS */
mix = getval("mix");
iphase = (int) (getval("phase") + 0.5);
slpwr = getval("slpwr");
slpw = getval("slpw");
getstr("sspul", sspul);
hsglvl = getval("hsglvl");
hsgt = getval("hsgt");
gzlvlz = getval("gzlvlz");
gtz = getval("gtz");
zfpwr = getval("zfpwr");
zfpw = getval("zfpw");
getstr("zfshp",zfshp);
getstr("zfilt",zfilt);
getstr("composit",composit);
getstr("compshape",compshape);
sub(ct,ssctr,v7);
settable(t1,4,ph1); getelem(t1,v7,v1);
settable(t2,8,ph2); getelem(t2,v7,v2); add(v2,two,v3);
settable(t3,8,ph3); getelem(t3,v7,oph);
settable(t4,8,ph4); getelem(t4,v7,v4);
settable(t5,4,ph5); getelem(t5,v7,v5);
settable(t6,8,ph6); getelem(t5,v7,v6);
if (zfilt[0] == 'n') assign(v1,oph);
if (iphase == 2)
{incr(v1); incr(v6);} /* hypercomplex method */
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v13);
add(v1,v13,v1);
add(v6,v13,v6);
add(oph,v13,oph);
cycles = mix / (4.0 * slpw);
initval(cycles, v10); /* mixing time cycles */
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
delay(5.0e-6);
if (sspul[0] == 'y')
{
zgradpulse(hsglvl,hsgt);
rgpulse(pw,zero,rof1,rof1);
zgradpulse(hsglvl,hsgt);
}
delay(d1);
if (getflag("wet"))
wet4(zero,one);
status(B);
rgpulse(pw, v1, rof1, rof1);
if (d2 > (POWER_DELAY + (2.0*pw/PI) + rof1))
delay(d2 - POWER_DELAY - (2.0*pw/PI) - rof1);
else {
if (ix == 1)
dps_show("delay",d2);
else if ((ix > 2) && (iphase < 2))
text_error("increment %d cannot be timed properly\n", (int) ix/2);
}
obspower(slpwr);
if (cycles > 1.5000)
{
obsunblank(); xmtron();
starthardloop(v10);
txphase(v2);
delay(2*slpw);
txphase(v3);
delay(2*slpw);
endhardloop();
xmtroff(); obsblank();
}
if (zfilt[0] == 'y')
{
obspower(tpwr);
rgpulse(pw,v4,1.0e-6,rof1);
zgradpulse(gzlvlz,gtz);
delay(gtz/3);
obspower(zfpwr);
shaped_pulse(zfshp,zfpw,zero,2.0e-6,2.0e-6);
zgradpulse(gzlvlz/4,gtz/3);
obspower(tpwr);
delay(gtz/8);
if (composit[0] == 'y')
{
if (rfwg[OBSch-1] == 'y')
shaped_pulse(compshape,4.0*pw+0.8e-6,v5,rof1,rof2);
else
comp90pulse(pw,v5,rof1,rof2);
}
else
rgpulse(pw,v5,rof1,rof2);
}
else
delay(rof2);
status(C);
}
pulsesequence()
{
double slpwrT = getval("slpwrT"),
slpwT = getval("slpwT"),
mixT = getval("mixT"),
gzlvl1 = getval("gzlvl1"),
gt1 = getval("gt1"),
gzlvl2 = getval("gzlvl2"),
gt2 = getval("gt2"),
gstab = getval("gstab"),
selpwrA = getval("selpwrA"),
selpwA = getval("selpwA"),
gzlvlA = getval("gzlvlA"),
gtA = getval("gtA"),
selpwrB = getval("selpwrB"),
selpwB = getval("selpwB"),
gzlvlB = getval("gzlvlB"),
gtB = getval("gtB"),
selfrq = getval("selfrq"),
zqfpw1 = getval("zqfpw1"),
zqfpwr1 = getval("zqfpwr1"),
zqfpw2 = getval("zqfpw2"),
zqfpwr2 = getval("zqfpwr2"),
gzlvlzq1 = getval("gzlvlzq1"),
gzlvlzq2 = getval("gzlvlzq2"),
phincr1 = getval("phincr1");
char slpatT[MAXSTR], selshapeA[MAXSTR], selshapeB[MAXSTR], zqfpat1[MAXSTR],
zqfpat2[MAXSTR], flipback[MAXSTR], alt_grd[MAXSTR];
//synchronize gradients to srate for probetype='nano'
// Preserve gradient "area"
gtA = syncGradTime("gtA","gzlvlA",1.0);
gzlvlA = syncGradLvl("gtA","gzlvlA",1.0);
gtB = syncGradTime("gtB","gzlvlB",1.0);
gzlvlB = syncGradLvl("gtB","gzlvlB",1.0);
getstr("slpatT",slpatT);
getstr("selshapeA",selshapeA);
getstr("selshapeB",selshapeB);
getstr("zqfpat1",zqfpat1);
getstr("zqfpat2",zqfpat2);
getstr("flipback", flipback);
getstr("alt_grd",alt_grd);
/* alternate gradient sign on every 2nd transient */
if (strcmp(slpatT,"mlev17c") &&
strcmp(slpatT,"dipsi2") &&
strcmp(slpatT,"dipsi3") &&
strcmp(slpatT,"mlev17") &&
strcmp(slpatT,"mlev16"))
abort_message("SpinLock pattern %s not supported!.\n", slpatT);
/* STEADY-STATE PHASECYCLING */
/* This section determines if the phase calculations trigger off of (SS - SSCTR) or off of CT */
assign(ct,v17);
ifzero(ssctr);
assign(v17,v13);
elsenz(ssctr);
/* purge option does not adjust v13 during steady state*/
sub(ssval, ssctr, v13); /* v13 = 0,...,ss-1 */
endif(ssctr);
mod4(v13,v1); /* v1 = 0 1 2 3 */
hlv(v13,v13);
hlv(v13,v13);
mod4(v13,v11); /* v11 = 0000 1111 2222 3333 */
dbl(v1,oph);
add(v11,oph,oph);
add(v11,oph,oph); /* oph = 2v1 + 2v11 */
/* CYCLOPS */
hlv(v13,v13);
hlv(v13,v14);
add(v1,v14,v1);
add(v11,v14,v11);
add(oph,v14,oph);
assign(v14,v21);
add(one,v21,v21);
add(two,v21,v12);
if (phincr1 < 0.0) phincr1=360+phincr1;
initval(phincr1,v5);
mod2(ct,v2); /* 01 01 */
hlv(ct,v4); hlv(v4,v4); mod2(v4,v4); dbl(v4,v4); /* 0000 2222 */
add(v4,v2,v4); mod4(v4,v4); /* 0101 2323 first echo in Excitation Sculpting */
hlv(ct,v7); mod2(v7,v7); /* 0011 */
hlv(ct,v9); hlv(v9,v9); hlv(v9,v9); dbl(v9,v9); add(v9,v7,v9);
mod4(v9,v9); /* 0011 0011 2233 2233 second echo in Excitation Sculpting */
dbl(v2,v2); /* 0202 */
dbl(v7,v7); /* 0022 */
add(v2,v7,v7); /* 0220 correct oph for Excitation Sculpting */
add(oph,v7,oph); mod4(oph,oph);
if (alt_grd[0] == 'y') mod2(ct,v10); /* alternate gradient sign on every 2nd transient */
/* BEGIN THE ACTUAL PULSE SEQUENCE */
status(A);
if (getflag("lkgate_flg")) lk_sample(); /* turn lock sampling on */
obspower(tpwr);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
delay(d1);
if (getflag("lkgate_flg")) lk_hold(); /* turn lock sampling off */
status(B);
rgpulse(pw, v14, rof1, rof1);
if (selfrq != tof)
obsoffset(selfrq);
ifzero(v10); zgradpulse(gzlvlA,gtA);
elsenz(v10); zgradpulse(-gzlvlA,gtA); endif(v10);
delay(gstab);
obspower(selpwrA);
shaped_pulse(selshapeA,selpwA,v1,rof1,rof1);
obspower(tpwr);
ifzero(v10); zgradpulse(gzlvlA,gtA);
elsenz(v10); zgradpulse(-gzlvlA,gtA); endif(v10);
delay(gstab);
if (selfrq != tof)
delay(2*OFFSET_DELAY);
ifzero(v10); zgradpulse(gzlvlB,gtB);
elsenz(v10); zgradpulse(-gzlvlB,gtB); endif(v10);
delay(gstab);
obspower(selpwrB);
shaped_pulse(selshapeB,selpwB,v11,rof1,rof1);
obspower(tpwr);
ifzero(v10); zgradpulse(gzlvlB,gtB);
elsenz(v10); zgradpulse(-gzlvlB,gtB); endif(v10);
delay(gstab);
if (selfrq != tof)
obsoffset(tof);
rgpulse(pw, v14, rof1, rof1);
if (getflag("Gzqfilt"))
{
obspower(zqfpwr1);
ifzero(v10); rgradient('z',gzlvlzq1);
elsenz(v10); rgradient('z',-gzlvlzq1); endif(v10);
delay(100.0e-6);
shaped_pulse(zqfpat1,zqfpw1,v14,rof1,rof1);
delay(100.0e-6);
rgradient('z',0.0);
delay(gstab);
}
obspower(slpwrT);
ifzero(v10); zgradpulse(gzlvl1,gt1);
elsenz(v10); zgradpulse(-gzlvl1,gt1); endif(v10);
delay(gstab);
if (mixT > 0.0)
{
if (dps_flag)
rgpulse(mixT,v21,0.0,0.0);
else
SpinLock(slpatT,mixT,slpwT,v21);
}
if (getflag("Gzqfilt"))
{
obspower(zqfpwr2);
ifzero(v10); rgradient('z',gzlvlzq2);
elsenz(v10); rgradient('z',-gzlvlzq2); endif(v10);
delay(100.0e-6);
shaped_pulse(zqfpat2,zqfpw2,v14,rof1,rof1);
delay(100.0e-6);
rgradient('z',0.0);
delay(gstab);
}
obspower(tpwr);
ifzero(v10); zgradpulse(gzlvl2,gt2);
elsenz(v10); zgradpulse(-gzlvl2,gt2); endif(v10);
delay(gstab);
if (flipback[A] == 'y')
FlipBack(v14,v5);
rgpulse(pw,v14,rof1,2.0e-6);
ExcitationSculpting(v4,v9,v10);
delay(rof2);
status(C);
}
void pulsesequence()
{
/* DECLARE VARIABLES */
char C13refoc[MAXSTR],comp_flg[MAXSTR],fsat[MAXSTR],f1180[MAXSTR];
int phase,t1_counter;
double pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rf0, /* maximum fine power when using pwC pulses */
rfst, /* fine power for the stCall pulse */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
tau1, /* t1 delay */
taua, /* < 1 / 4J(NH) 2.25 ms */
taub, /* 1 / 4J(NH) in NH : 2.68 ms */
pwn, /* PW90 for N-nuc */
pwN, /* N15 pw90 for BioPack */
pwNlvl, /* N15 power for BioPack */
pwn_cp, /* PW90 for N CPMG */
pwHs, /* BioPack selective PW90 for water excitation */
compH, /* amplifier compression factor*/
compN, /* amplifier compression factor*/
phase_sl,
tsatpwr, /* low power level for presat */
tpwrsf_u, /* fine power adjustment on flip-up sel 90 */
tpwrsf_d, /* fine power adjustment on flip-down sel 90 */
tpwrsl, /* low power level for sel 90 */
dhpwr2, /* power level for N hard pulses */
dpwr2_comp, /* power level for CPMG compensation */
dpwr2_cp, /* power level for N CPMG */
tauCPMG, /* CPMG delay */
ncyc, /* number of times to loop */
ncyc_max, /* max number of times to loop */
time_T2, /* total time for T2 measuring */
tofps, /* water freq */
sw1,
pwr_delay, /* POWER_DELAY recalculated*/
timeC,
gt1,
gt2,
gt3,
gt4,
gt5,
gt6,
gstab, /* stabilization delay */
BPpwrlimits, /* =0 for no limit, =1 for limit */
gzlvl1,
gzlvl2,
gzlvl3,
gzlvl4,
gzlvl5,
gzlvl6;
P_getreal(GLOBAL,"BPpwrlimits",&BPpwrlimits,1);
/* LOAD VARIABLES */
getstr("C13refoc", C13refoc);
/* taub = 1/(8*93.0); */
taua = getval("taua");
taub = getval("taub");
pwNlvl = getval("pwNlvl");
pwN = getval("pwN");
pwn = getval("pwn");
pwn_cp = getval("pwn_cp");
pwHs = getval("pwHs");
compH = getval("compH");
compN = getval("compN");
phase_sl = getval("phase_sl");
tsatpwr = getval("tsatpwr");
tpwrsf_u = getval("tpwrsf_u");
tpwrsf_d = getval("tpwrsf_d");
tpwrsl = getval("tpwrsl");
dhpwr2 = getval("dhpwr2");
dpwr2_comp = getval("dpwr2_comp");
dpwr2_cp = getval("dpwr2_cp");
ncyc = getval("ncyc");
ncyc_max = getval("ncyc_max");
time_T2 = getval("time_T2");
phase = (int) (getval("phase") + 0.5);
sw1 = getval("sw1");
tofps = getval("tofps");
gt1 = getval("gt1");
gt2 = getval("gt2");
gt3 = getval("gt3");
gt4 = getval("gt4");
gt5 = getval("gt5");
gt6 = getval("gt6");
gstab = getval("gstab");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl3 = getval("gzlvl3");
gzlvl4 = getval("gzlvl4");
gzlvl5 = getval("gzlvl5");
gzlvl6 = getval("gzlvl6");
getstr("fsat",fsat);
getstr("comp_flg",comp_flg);
getstr("f1180",f1180);
setautocal(); /* activate auto-calibration flags */
if (autocal[0] == 'n')
{
/* selective H20 one-lobe sinc pulse */
if (pwHs > 0.0)
tpwrsl = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /*needs 1.69 times more*/
else tpwrsl = 0.0;
tpwrsl = (int) (tpwrsl); /*power than a square pulse */
}
else /* if autocal = 'y'(yes), 'q'(quiet), r(read), or 's'(semi) */
{
if(FIRST_FID) /* call Pbox */
{
H2OsincA = pbox_Rsh("H2OsincA", "sinc90", pwHs, 0.0, compH*pw, tpwr);
ofs_check(H1ofs, C13ofs, N15ofs, H2ofs);
}
pwHs = H2OsincA.pw; tpwrsl = H2OsincA.pwr-1.0; /* 1dB correction applied */
pwn = pwN; dhpwr2 = pwNlvl;
}
if (tpwrsf_u < 4095.0)
{
tpwrsl = tpwrsl + 6.0;
pwr_delay = POWER_DELAY + PWRF_DELAY;
}
else pwr_delay = POWER_DELAY;
/* maximum fine power for pwC pulses (and initialize rfst) */
rf0 = 4095.0; rfst=0.0;
/* 180 degree adiabatic C13 pulse from 0 to 200 ppm */
if (C13refoc[A]=='y')
{rfst = (compC*4095.0*pwC*4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35));
rfst = (int) (rfst + 0.5);
if ( 1.0/(4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35)) < pwC )
{ text_error( " Not enough C13 RF. pwC must be %f usec or less.\n",
(1.0e6/(4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35))) ); psg_abort(1); }}
/* check validity of parameter range */
if(dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y')
{
printf("incorrect Dec1 decoupler flags! Should be nnn ");
psg_abort(1);
}
if(dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y' )
{
printf("incorrect Dec2 decoupler flags! Should be nnn ");
psg_abort(1);
}
if( tsatpwr > 8 )
{
printf("tsatpwr too large !!! ");
psg_abort(1);
}
if( dpwr2_cp > 61 )
{
printf("don't fry the probe, dpwr2_cp too large for cpmg !");
psg_abort(1);
}
if( ncyc > 100)
{
printf("ncyc exceeds 100. May be too much \n");
psg_abort(1);
}
if( time_T2 > 0.090 )
{
printf("total T2 recovery time exceeds 90 msec. May be too long \n");
psg_abort(1);
}
if( ncyc > 0)
{
tauCPMG = time_T2/(4*ncyc) - pwn_cp;
if( ix == 1 )
printf("nuCPMG for current experiment is (Hz): %5.3f \n",1/(4*(tauCPMG+pwn_cp)) );
}
else
{
tauCPMG = time_T2/4 - pwn_cp;
if( ix == 1 )
printf("nuCPMG for current experiment is (Hz): not applicable \n");
}
ncyc_max = time_T2/1e-3;
if( tauCPMG + pwn_cp < 0.000250)
{
printf("WARNING: value of tauCPMG must be larger than or equal to 250 us\n");
printf("maximum value of ncyc allowed for current time_T2 is: %5.2f \n",ncyc_max);
psg_abort(1);
}
if(gt1 > 3e-3 || gt2 > 3e-3 || gt3 > 3e-3|| gt4 > 3e-3
|| gt5 > 3e-3 || gt6 > 3e-3 )
{
printf("gti must be less than 3e-3\n");
psg_abort(1);
}
/* LOAD VARIABLES */
settable(t1, 2, phi1);
settable(t2, 8, phi2);
settable(t3, 8, phi3);
settable(t4, 1, phi4);
settable(t5, 1, phi5);
settable(t6, 1, phi6);
settable(t7, 8, rec);
/* Phase incrementation for hypercomplex 2D data */
if (phase == 2) {
tsadd(t4,2,4);
tsadd(t5,2,4);
tsadd(t6,2,4);
tsadd(t7,2,4);
}
/* Set up f1180 */
tau1 = d2;
if(f1180[A] == 'y')
tau1 += ( 1.0 / (2.0*sw1) - (pw + pwN*2.0/3.1415));
else
tau1 = tau1 - pw;
if(tau1 < 0.2e-6) tau1 = 0.2e-6;
tau1 = tau1/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1 ) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if( t1_counter %2 ) {
tsadd(t2,2,4);
tsadd(t3,2,4);
tsadd(t7,2,4);
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
decpower(dpwr); /* Set decoupler1 power to dpwr */
decpower(pwClvl);
decpwrf(rfst);
decoffset(dof);
dec2power(dhpwr2); /* Set decoupler2 power to dhpwr2 */
/* Presaturation Period */
if(fsat[0] == 'y')
{
obspower(tsatpwr); /* Set power for presaturation */
obsoffset(tofps); /* move H carrier to the water */
rgpulse(d1,zero,rof1,rof1); /* presat. with transmitter */
obspower(tpwr); /* Set power for hard pulses */
}
else
{
obspower(tpwr); /* Set power for hard pulses */
delay(d1);
}
obsoffset(tof);
status(B);
/* apply the compensation 15N pulses if desired */
if(comp_flg[A] == 'y') {
dec2power(dpwr2_comp); /* Set decoupler2 compensation power */
timeC = time_T2*(ncyc_max-ncyc)/ncyc_max;
dec2rgpulse(timeC,zero,0.0,0.0);
dec2power(dhpwr2);
}
rcvroff();
delay(20.0e-6);
/* shaped pulse on water */
obspower(tpwrsl);
if (tpwrsf_d<4095.0) obspwrf(tpwrsf_d);
if (autocal[A] == 'y')
shaped_pulse("H2OsincA",pwHs,three,rof1,0.0);
else
shaped_pulse("H2Osinc_d",pwHs,three,rof1,0.0);
if (tpwrsf_d<4095.0) obspwrf(4095.0);
obspower(tpwr);
/* shaped pulse on water */
rgpulse(pw,two,rof1,0.0);
txphase(zero);
dec2phase(zero);
delay(2.0e-6);
zgradpulse(gzlvl1,gt1);
delay(gstab);
delay(taua - gt1 - gstab -2.0e-6); /* delay < 1/4J(XH) */
sim3pulse(2*pw,0.0e-6,2*pwn,zero,zero,zero,0.0,0.0);
txphase(one);
dec2phase(t1);
delay(taua - gt1 - gstab -2.0e-6);
delay(2.0e-6);
zgradpulse(gzlvl1,gt1);
delay(gstab);
rgpulse(pw,one,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl2,gt2);
delay(gstab);
if (BPpwrlimits > 0.5)
{
dec2power(dpwr2_cp -3.0); /* reduce for probe protection */
pwn_cp=pwn_cp*compN*1.4;
}
else
dec2power(dpwr2_cp); /* Set decoupler2 power to dpwr2_cp for CPMG period */
dec2rgpulse(pwn_cp,t1,rof1,2.0e-6);
dec2phase(zero);
/* start of the CPMG train for first period time_T2/2 on Ny(1-2Hz) */
if(ncyc > 0)
{
delay(tauCPMG - (2/PI)*pwn_cp - 2.0e-6);
dec2rgpulse(2*pwn_cp,one,0.0,0.0);
delay(tauCPMG);
}
if(ncyc > 1)
{
initval(ncyc-1,v4);
loop(v4,v5);
delay(tauCPMG);
dec2rgpulse(2*pwn_cp,one,0.0,0.0);
delay(tauCPMG);
endloop(v5);
}
/* eliminate cross-relaxation */
delay(2.0e-6);
zgradpulse(gzlvl3,gt3);
delay(gstab);
delay(taub - gt3 - gstab -2.0e-6 - pwn_cp);
/* composite 1H 90y-180x-90y on top of 15N 180x */
dec2rgpulse(pwn_cp-2*pw,zero,0.0e-6,0.0);
sim3pulse(pw,0.0e-6,pw,one,zero,zero,0.0,0.0);
sim3pulse(2*pw,0.0e-6,2*pw,zero,zero,zero,0.0,0.0);
sim3pulse(pw,0.0e-6,pw,one,zero,zero,0.0,0.0);
dec2rgpulse(pwn_cp-2*pw,zero,0.0,0.0e-6);
/* composite 1H 90y-180x-90y on top of 15N 180x */
delay(taub - gt3 - gstab -2.0e-6 - pwn_cp - 4.0*pw);
delay(2.0e-6);
zgradpulse(gzlvl3,gt3);
delay(gstab);
rgpulse(pw,one,0.0,0.0);
rgpulse(2.0*pw,zero,0.0,0.0);
rgpulse(pw,one,0.0,0.0);
/* start of the CPMG train for second period time_T2/2 on Nx(1-2Iz) */
if(ncyc > 1)
{
initval(ncyc-1,v4);
loop(v4,v5);
delay(tauCPMG);
dec2rgpulse(2*pwn_cp,zero,0.0,0.0);
delay(tauCPMG);
endloop(v5);
}
if(ncyc > 0)
{
delay(tauCPMG);
dec2rgpulse(2*pwn_cp,zero,0.0,0.0);
delay(tauCPMG - (2/PI)*pwn_cp - 2.0e-6);
}
dec2phase(one);
dec2rgpulse(pwn_cp,one,2.0e-6,0.0);
delay(rof1);
dec2power(dhpwr2); /* Set decoupler2 power back to dhpwr2 */
dec2phase(t3);
delay(2.0e-6);
zgradpulse(gzlvl4,gt4);
delay(gstab);
if(phase==1)
dec2rgpulse(pwn,t2,rof1,0.0);
if(phase==2)
dec2rgpulse(pwn,t3,rof1,0.0);
txphase(t4);
decphase(one);
dec2phase(zero);
/* 15N chemical shift labeling with optional 13C decoupling of Ca & C'*/
if ( (C13refoc[A]=='y') && (tau1 > 0.5e-3 + WFG2_START_DELAY) )
{delay(tau1 - 0.5e-3 - WFG2_START_DELAY); /* WFG2_START_DELAY */
decshaped_pulse("stC200", 1.0e-3, zero, 0.0, 0.0);
delay(tau1 - 0.5e-3);}
else delay(2.0*tau1);
/* finish of 15N shift labeling*/
rgpulse(pw,t4,0.0,0.0);
/* shaped pulse on water */
obspower(tpwrsl);
if (tpwrsf_u<4095.0) obspwrf(tpwrsf_u);
if (autocal[A] == 'y')
shaped_pulse("H2OsincA",pwHs,t5,rof1,0.0);
else
shaped_pulse("H2Osinc_u",pwHs,t5,rof1,0.0);
if (tpwrsf_u<4095.0) obspwrf(4095.0);
obspower(tpwr);
/* shaped pulse on water */
delay(2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(gstab/2.0);
delay(taua - pwr_delay - rof1 - WFG_START_DELAY
- pwHs - WFG_STOP_DELAY - pwr_delay
- gt5 - gstab/2.0 -2.0e-6);
sim3pulse(2.0*pw,0.0,2.0*pwn,zero,zero,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(gstab/2.0);
delay(taua
- gt5 - 2.0e-6 -gstab
- pwr_delay - rof1 - WFG_START_DELAY
- pwHs - WFG_STOP_DELAY - pwr_delay - 2.0e-6);
/* shaped pulse on water */
obspower(tpwrsl);
if (tpwrsf_u<4095.0) obspwrf(tpwrsf_u);
if (autocal[A] == 'y')
shaped_pulse("H2OsincA",pwHs,zero,rof1,0.0);
else
shaped_pulse("H2Osinc_u",pwHs,zero,rof1,0.0);
if (tpwrsf_u<4095.0) obspwrf(4095.0);
obspower(tpwr);
/* shaped pulse on water */
sim3pulse(pw,0.0e-6,pwn,zero,zero,t6,2.0e-6,0.0);
txphase(zero);
dec2phase(zero);
delay(2.0e-6);
zgradpulse(gzlvl6,gt6);
delay(gstab/2.0);
delay(taua - gt6 - gstab/2.0 -2.0e-6 - pwr_delay
- pwHs);
initval(1.0,v3);
obsstepsize(phase_sl);
xmtrphase(v3);
obspower(tpwrsl);
if (tpwrsf_d<4095.0) obspwrf(tpwrsf_d);
if (autocal[A] == 'y')
shaped_pulse("H2OsincA",pwHs,two,rof1,0.0);
else
shaped_pulse("H2Osinc_d",pwHs,two,rof1,0.0);
if (tpwrsf_d<4095.0) obspwrf(4095.0);
obspower(tpwr);
xmtrphase(zero);
sim3pulse(2*pw,0.0e-6,2*pwn,zero,zero,zero,rof1,rof1);
initval(1.0,v3);
obsstepsize(phase_sl);
xmtrphase(v3);
obspower(tpwrsl);
if (tpwrsf_u<4095.0) obspwrf(tpwrsf_u);
if (autocal[A] == 'y')
shaped_pulse("H2OsincA",pwHs,two,rof1,0.0);
else
shaped_pulse("H2Osinc_u",pwHs,two,rof1,0.0);
if (tpwrsf_u<4095.0) obspwrf(4095.0);
obspower(tpwr);
xmtrphase(zero);
delay(2.0e-6);
zgradpulse(gzlvl6,gt6);
delay(gstab/2.0);
delay(taua - pwHs - gt6 - gstab/2.0 -2.0e-6
+ 2.0*pw/PI - pwn
- 2.0*POWER_DELAY);
dec2rgpulse(pwn,zero,0.0,0.0);
decpower(dpwr); /* lower power on dec */
dec2power(dpwr2); /* lower power on dec2 */
/* acquire data */
status(C);
setreceiver(t7);
}
void pulsesequence()
{
/* DECLARE AND LOAD VARIABLES */
char f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
mag_flg[MAXSTR], /* magic-angle coherence transfer gradients */
TROSY[MAXSTR]; /* do TROSY on N15 and H1 */
int icosel, /* used to get n and p type */
t1_counter=getval("t1_counter"), /* used for states tppi in t1 */
t2_counter=getval("t2_counter"), /* used for states tppi in t2 */
nli = getval("nli"),
nli2 = getval("nli2");
double tau1, /* t1 delay */
tau2, /* t2 delay */
timeTN = getval("timeTN"), /* constant time for 15N evolution */
kappa = 5.4e-3,
lambda = 2.4e-3,
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rf0, /* maximum fine power when using pwC pulses */
/* the following pulse lengths for SLP pulses are automatically calculated */
/* by the macro "proteincal". SLP pulse shapes, "offC3" etc are called */
/* directly from your shapelib. */
pwC3 = getval("pwC3"), /*180 degree pulse at Ca(56ppm) null at CO(174ppm) */
pwC3a = getval("pwC3a"), /* pwC3a=pwC3, but not set to zero when pwC3=0 */
phshift3, /* phase shift induced on CO by pwC3 ("offC3") pulse */
pwZ, /* the largest of pwC3 and 2.0*pwN */
pwZ1, /* the largest of pwC3a and 2.0*pwN for 1D experiments */
pwC6 = getval("pwC6"), /* 90 degree selective sinc pulse on CO(174ppm) */
pwC8 = getval("pwC8"), /* 180 degree selective sinc pulse on CO(174ppm) */
rf3, /* fine power for the pwC3 ("offC3") pulse */
rf6, /* fine power for the pwC6 ("offC6") pulse */
rf8, /* fine power for the pwC8 ("offC8") pulse */
compH = getval("compH"), /* adjustment for C13 amplifier compression */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
pwHs = getval("pwHs"), /* H1 90 degree pulse length at tpwrs */
tpwrsf = getval("tpwrsf"), /* fine power for pwHs pulse */
tpwrs, /* power for the pwHs ("H2Osinc") pulse */
pwHd, /* H1 90 degree pulse length at tpwrd */
tpwrd, /* rf for WALTZ decoupling */
waltzB1 = getval("waltzB1"), /* waltz16 field strength (in Hz) */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
sw1 = getval("sw1"),
sw2 = getval("sw2"),
gt1 = getval("gt1"), /* coherence pathway gradients */
gzcal = getval("gzcal"), /* g/cm to DAC conversion factor */
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gt0 = getval("gt0"), /* other gradients */
gt3 = getval("gt3"),
gt4 = getval("gt4"),
gt5 = getval("gt5"),
gstab = getval("gstab"),
gzlvl0 = getval("gzlvl0"),
gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5"),
gzlvl6 = getval("gzlvl6");
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("mag_flg",mag_flg);
getstr("TROSY",TROSY);
/* LOAD PHASE TABLE */
settable(t3,2,phi3);
settable(t4,1,phx);
settable(t5,4,phi5);
if (TROSY[A]=='y')
{settable(t8,1,phy);
settable(t9,1,phx);
settable(t10,1,phy);
settable(t11,1,phx);
settable(t12,4,recT);}
else
{settable(t8,1,phx);
settable(t9,8,phi9);
settable(t10,1,phx);
settable(t11,1,phy);
settable(t12,4,rec);}
/* INITIALIZE VARIABLES */
if( dpwrf < 4095 )
{ printf("reset dpwrf=4095 and recalibrate C13 90 degree pulse");
psg_abort(1); }
/* maximum fine power for pwC pulses */
rf0 = 4095.0;
/* 180 degree pulse on Ca, null at CO 118ppm away */
rf3 = (compC*4095.0*pwC*2.0)/pwC3a;
rf3 = (int) (rf3 + 0.5);
/* the pwC3 pulse at the middle of t1 */
if ((nli2 > 0.0) && (nli == 1.0)) nli = 0.0;
if (pwC3a > 2.0*pwN) pwZ = pwC3a; else pwZ = 2.0*pwN;
if ((pwC3==0.0) && (pwC3a>2.0*pwN)) pwZ1=pwC3a-2.0*pwN; else pwZ1=0.0;
if ( nli > 1 ) pwC3 = pwC3a;
if ( pwC3 > 0 ) phshift3 = 48.0;
else phshift3 = 0.0;
/* 90 degree one-lobe sinc pulse on CO, null at Ca 118ppm away */
rf6 = (compC*4095.0*pwC*1.69)/pwC6; /* needs 1.69 times more */
rf6 = (int) (rf6 + 0.5); /* power than a square pulse */
/* 180 degree one-lobe sinc pulse on CO, null at Ca 118ppm away */
rf8 = (compC*4095.0*pwC*2.0*1.65)/pwC8; /* needs 1.65 times more */
rf8 = (int) (rf8 + 0.5); /* power than a square pulse */
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /* needs 1.69 times more */
tpwrs = (int) (tpwrs); /* power than a square pulse */
/* power level and pulse time for WALTZ 1H decoupling */
pwHd = 1/(4.0 * waltzB1) ;
tpwrd = tpwr - 20.0*log10(pwHd/(compH*pw));
tpwrd = (int) (tpwrd + 0.5);
/* CHECK VALIDITY OF PARAMETER RANGES */
if ( 0.5*nli2*1/(sw2) > timeTN - WFG3_START_DELAY)
{ printf(" nli2 is too big. Make nli2 equal to %d or less.\n",
((int)((timeTN - WFG3_START_DELAY)*2.0*sw2))); psg_abort(1);}
if ( dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y' )
{ printf("incorrect dec1 decoupler flags! Should be 'nnn' "); psg_abort(1);}
if ( dm2[A] == 'y' || dm2[B] == 'y' )
{ printf("incorrect dec2 decoupler flags! Should be 'nny' "); psg_abort(1);}
if ( dpwr2 > 46 )
{ printf("dpwr2 too large! recheck value "); psg_abort(1);}
if ( pw > 50.0e-6 )
{ printf(" pw too long ! recheck value "); psg_abort(1);}
if ( (pwN > 100.0e-6) && (nli>1 || nli2>1))
{ printf(" pwN too long! recheck value "); psg_abort(1);}
if ( TROSY[A]=='y' && dm2[C] == 'y' )
{ text_error("Choose either TROSY='n' or dm2='n' ! "); psg_abort(1);}
/* PHASES AND INCREMENTED TIMES */
/* Phase incrementation for hypercomplex 2D data, States-Haberkorn element */
if (phase1 == 2) tsadd(t3,1,4);
if (TROSY[A]=='y')
{ if (phase2 == 2) icosel = +1;
else {tsadd(t4,2,4); tsadd(t10,2,4); icosel = -1;}
}
else { if (phase2 == 2) {tsadd(t10,2,4); icosel = +1;}
else icosel = -1;
}
/* Set up f1180 */
if( ix == 1) d2_init = d2;
tau1 = d2_init + (t1_counter) / sw1;
if((f1180[A] == 'y') && (nli > 1.0))
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
tau1 = tau1/2.0;
/* Set up f2180 */
if( ix == 1) d3_init = d3;
tau2 = d3_init + (t2_counter) / sw2;
if((f2180[A] == 'y') && (nli2 > 1.0))
{ tau2 += ( 1.0 / (2.0*sw2) ); if(tau2 < 0.2e-6) tau2 = 0.0; }
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if(t1_counter % 2)
{ tsadd(t3,2,4); tsadd(t12,2,4); }
if(t2_counter % 2)
{ tsadd(t8,2,4); tsadd(t12,2,4); }
/* BEGIN PULSE SEQUENCE */
status(A);
delay(d1);
rcvroff();
obspower(tpwr);
decpower(pwClvl);
dec2power(pwNlvl);
decpwrf(rf0);
obsoffset(tof);
txphase(zero);
delay(1.0e-5);
if (TROSY[A] == 'n')
dec2rgpulse(pwN, zero, 0.0, 0.0); /*destroy N15 and C13 magnetization*/
decrgpulse(pwC, zero, 0.0, 0.0);
zgradpulse(gzlvl0, 0.5e-3);
delay(1.0e-4);
if (TROSY[A] == 'n')
dec2rgpulse(pwN, one, 0.0, 0.0);
decrgpulse(pwC, zero, 0.0, 0.0);
zgradpulse(0.7*gzlvl0, 0.5e-3);
delay(5.0e-4);
rgpulse(pw,zero,0.0,0.0); /* 1H pulse excitation */
dec2phase(zero);
zgradpulse(gzlvl0, gt0);
delay(lambda - gt0);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
txphase(one);
zgradpulse(gzlvl0, gt0);
delay(lambda - gt0);
rgpulse(pw, one, 0.0, 0.0);
if (tpwrsf < 4095.0)
{obspwrf(tpwrsf); tpwrs=tpwrs+6.0;}
obspower(tpwrs);
if (TROSY[A]=='y')
{txphase(two);
shaped_pulse("H2Osinc", pwHs, two, 5.0e-4, 0.0);
obspower(tpwr); obspwrf(4095.0);
zgradpulse(gzlvl3, gt3);
delay(2.0e-4);
dec2rgpulse(pwN, zero, 0.0, 0.0);
delay(0.5*kappa - 2.0*pw);
rgpulse(2.0*pw, two, 0.0, 0.0);
decphase(zero);
dec2phase(zero);
decpwrf(rf8);
delay(timeTN - 0.5*kappa - WFG3_START_DELAY);
}
else
{txphase(zero);
shaped_pulse("H2Osinc", pwHs, zero, 5.0e-4, 0.0);
obspower(tpwrd); obspwrf(4095.0);
zgradpulse(gzlvl3, gt3);
delay(2.0e-4);
dec2rgpulse(pwN, zero, 0.0, 0.0);
txphase(one);
delay(kappa - pwHd - 2.0e-6 - PRG_START_DELAY);
rgpulse(pwHd,one,0.0,0.0);
txphase(zero);
delay(2.0e-6);
obsprgon("waltz16", pwHd, 90.0); /* PRG_START_DELAY */
xmtron();
decphase(zero);
dec2phase(zero);
decpwrf(rf8);
delay(timeTN - kappa - WFG3_START_DELAY);
}
/* WFG3_START_DELAY */
sim3shaped_pulse("", "offC8", "", 0.0, pwC8, 2.0*pwN, zero, zero, zero,
0.0, 0.0);
decphase(t3);
decpwrf(rf6);
delay(timeTN);
dec2rgpulse(pwN, zero, 0.0, 0.0);
if (TROSY[A]=='n')
{xmtroff();
obsprgoff();
rgpulse(pwHd,three,2.0e-6,0.0);}
zgradpulse(gzlvl3, gt3);
delay(2.0e-4);
decshaped_pulse("offC6", pwC6, t3, 0.0, 0.0);
decphase(zero);
/* xxxxxxxxxxxxxxxxxxxxxx 13CO EVOLUTION xxxxxxxxxxxxxxxxxx */
if ((nli>1.0) && (tau1>0.0)) /* total 13C evolution equals d2 exactly */
{ /* 13C evolution during pwC6 is at 60% rate */
decpwrf(rf3);
if(tau1 - 0.6*pwC6 - WFG3_START_DELAY - 0.5*pwZ > 0.0)
{
delay(tau1 - 0.6*pwC6 - WFG3_START_DELAY - 0.5*pwZ);
/* WFG3_START_DELAY */
sim3shaped_pulse("", "offC3", "", 0.0, pwC3a, 2.0*pwN, zero, zero, zero,
0.0, 0.0);
initval(phshift3, v3);
decstepsize(1.0);
dcplrphase(v3); /* SAPS_DELAY */
delay(tau1 - 0.6*pwC6 - SAPS_DELAY - 0.5*pwZ- WFG_START_DELAY - 2.0e-6);
}
else
{
initval(180.0, v3);
decstepsize(1.0);
dcplrphase(v3); /* SAPS_DELAY */
delay(2.0*tau1 - 2.0*0.6*pwC6 - SAPS_DELAY - WFG_START_DELAY - 2.0e-6);
}
}
else if ((nli==1.0) && (pwC3==1.0e-6)) /* 13CO evolution for dof calib. */
{
decpwrf(rf8);
delay((1.0/(dfrq*80.0)) + 2.0e-6); /* WFG_START_DELAY */
decshaped_pulse("offC8", pwC8, zero, 0.0, 0.0);
}
else if (nli==1.0) /* special 1D check of pwC3 phase enabled when nli=1 */
{
decpwrf(rf3);
delay(10.0e-6 + SAPS_DELAY + 0.5*pwZ1 + WFG_START_DELAY);
/* WFG3_START_DELAY */
sim3shaped_pulse("", "offC3", "", 0.0, pwC3, 2.0*pwN, zero, zero, zero,
2.0e-6 , 0.0);
initval(phshift3, v3);
decstepsize(1.0);
dcplrphase(v3); /* SAPS_DELAY */
delay(10.0e-6 + WFG3_START_DELAY + 0.5*pwZ1);
}
else /* 13CO evolution refocused for 1st increment, or when nli=0 */
{
decpwrf(rf8);
delay(12.0e-6); /* WFG_START_DELAY */
decshaped_pulse("offC8", pwC8, zero, 0.0, 0.0);
delay(10.0e-6);
}
decphase(t5);
decpwrf(rf6);
delay(2.0e-6); /* WFG_START_DELAY */
decshaped_pulse("offC6", pwC6, t5, 0.0, 0.0);
/* xxxxxxxxxxxxxxxxxx OPTIONS FOR N15 EVOLUTION xxxxxxxxxxxxxxxxxxxxx */
dec2phase(t8);
zgradpulse(gzlvl4, gt4);
txphase(one);
dcplrphase(zero);
delay(2.0e-4);
if (TROSY[A]=='n')
{rgpulse(pwHd,one,0.0,0.0);
txphase(zero);
delay(2.0e-6);
obsprgon("waltz16", pwHd, 90.0);
xmtron();}
dec2rgpulse(pwN, t8, 0.0, 0.0);
decphase(zero);
dec2phase(t9);
decpwrf(rf8);
delay(timeTN - WFG3_START_DELAY - tau2);
/* WFG3_START_DELAY */
sim3shaped_pulse("", "offC8", "", 0.0, pwC8, 2.0*pwN, zero, zero, t9,
0.0, 0.0);
dec2phase(t10);
decpwrf(rf3);
if (TROSY[A]=='y')
{ if (tau2 > gt1 + 2.0*GRADIENT_DELAY + 1.5e-4 + pwHs)
{
txphase(three);
delay(timeTN - pwC3a - WFG_START_DELAY); /* WFG_START_DELAY */
decshaped_pulse("offC3", pwC3a, zero, 0.0, 0.0);
delay(tau2 - gt1 - 2.0*GRADIENT_DELAY - 1.5e-4 - pwHs);
if (mag_flg[A]=='y') magradpulse(icosel*gzcal*gzlvl1, gt1);
else zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
obspower(tpwrs);
if (tpwrsf<4095.0)
{obspwrf(tpwrsf); /* POWER_DELAY */
delay(1.0e-4 - POWER_DELAY - PWRF_DELAY);}
else
delay(1.0e-4 - POWER_DELAY);
shaped_pulse("H2Osinc", pwHs, three, 0.0, 0.0);
txphase(t4);
obspower(tpwr);
if (tpwrsf<4095.0)
{obspwrf(4095.0); /* POWER_DELAY */
delay(0.50e-4 - POWER_DELAY - PWRF_DELAY);}
else
delay(0.50e-4 - POWER_DELAY);
}
else if (tau2 > pwHs + 0.5e-4)
{
txphase(three);
delay(timeTN-pwC3a-WFG_START_DELAY-gt1-2.0*GRADIENT_DELAY-1.0e-4);
if (mag_flg[A]=='y') magradpulse(icosel*gzcal*gzlvl1, gt1);
else zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
obspower(tpwrs);
if (tpwrsf<4095.0)
{obspwrf(tpwrsf); /* POWER_DELAY */
delay(1.0e-4 - POWER_DELAY - PWRF_DELAY);}
else
delay(1.0e-4 - POWER_DELAY);
decshaped_pulse("offC3", pwC3a, zero, 0.0, 0.0);
delay(tau2 - pwHs - 0.5e-4);
shaped_pulse("H2Osinc", pwHs, three, 0.0, 0.0);
txphase(t4);
obspower(tpwr);
if (tpwrsf<4095.0)
{obspwrf(4095.0); /* POWER_DELAY */
delay(0.50e-4 - POWER_DELAY - PWRF_DELAY);}
else
delay(0.50e-4 - POWER_DELAY);
}
else
{
txphase(three);
delay(timeTN - pwC3a - WFG_START_DELAY - gt1 - 2.0*GRADIENT_DELAY
- 1.5e-4 - pwHs);
if (mag_flg[A]=='y') magradpulse(icosel*gzcal*gzlvl1, gt1);
else zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
obspower(tpwrs);
if (tpwrsf<4095.0)
{obspwrf(tpwrsf); /* POWER_DELAY */
delay(1.0e-4 - POWER_DELAY - PWRF_DELAY);}
else
delay(1.0e-4 - POWER_DELAY);
shaped_pulse("H2Osinc", pwHs, three, 0.0, 0.0);
txphase(t4);
decshaped_pulse("offC3", pwC3a, zero, 0.0, 0.0);
delay(tau2);
obspower(tpwr);
if (tpwrsf<4095.0)
{obspwrf(4095.0); /* POWER_DELAY */
delay(0.50e-4 - POWER_DELAY - PWRF_DELAY);}
else
delay(0.50e-4 - POWER_DELAY);
}
}
else
{
if (tau2 > kappa)
{
delay(timeTN - pwC3a - WFG_START_DELAY); /* WFG_START_DELAY */
decshaped_pulse("offC3", pwC3a, zero, 0.0, 0.0);
delay(tau2 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6);
xmtroff();
obsprgoff(); /* PRG_STOP_DELAY */
rgpulse(pwHd,three,2.0e-6,0.0);
txphase(t4);
delay(kappa - gt1 - 2.0*GRADIENT_DELAY - 1.0e-4);
if (mag_flg[A]=='y') magradpulse(icosel*gzcal*gzlvl1, gt1);
else zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
obspower(tpwr); /* POWER_DELAY */
delay(1.0e-4 - POWER_DELAY);
}
else if (tau2 > (kappa - pwC3a - WFG_START_DELAY))
{
delay(timeTN + tau2 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6);
xmtroff();
obsprgoff(); /* PRG_STOP_DELAY */
rgpulse(pwHd,three,2.0e-6,0.0);
txphase(t4); /* WFG_START_DELAY */
decshaped_pulse("offC3", pwC3a, zero, 0.0, 0.0);
delay(kappa -pwC3a -WFG_START_DELAY -gt1 -2.0*GRADIENT_DELAY -1.0e-4);
if (mag_flg[A]=='y') magradpulse(icosel*gzcal*gzlvl1, gt1);
else zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
obspower(tpwr); /* POWER_DELAY */
delay(1.0e-4 - POWER_DELAY);
}
else if (tau2 > gt1 + 2.0*GRADIENT_DELAY + 1.0e-4)
{
delay(timeTN + tau2 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6);
xmtroff();
obsprgoff(); /* PRG_STOP_DELAY */
rgpulse(pwHd,three,2.0e-6,0.0);
txphase(t4);
delay(kappa - tau2 - pwC3a - WFG_START_DELAY); /* WFG_START_DELAY */
decshaped_pulse("offC3", pwC3a, zero, 0.0, 0.0);
delay(tau2 - gt1 - 2.0*GRADIENT_DELAY - 1.0e-4);
if (mag_flg[A]=='y') magradpulse(icosel*gzcal*gzlvl1, gt1);
else zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
obspower(tpwr); /* POWER_DELAY */
delay(1.0e-4 - POWER_DELAY);
}
else
{
delay(timeTN + tau2 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6);
xmtroff();
obsprgoff(); /* PRG_STOP_DELAY */
rgpulse(pwHd,three,2.0e-6,0.0);
txphase(t4);
delay(kappa-tau2-pwC3a-WFG_START_DELAY-gt1-2.0*GRADIENT_DELAY-1.0e-4);
if (mag_flg[A]=='y') magradpulse(icosel*gzcal*gzlvl1, gt1);
else zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
obspower(tpwr); /* POWER_DELAY */
delay(1.0e-4 - POWER_DELAY); /* WFG_START_DELAY */
decshaped_pulse("offC3", pwC3a, zero, 0.0, 0.0);
delay(tau2);
}
}
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
if (TROSY[A]=='y') rgpulse(pw, t4, 0.0, 0.0);
else sim3pulse(pw, 0.0, pwN, t4, zero, t10, 0.0, 0.0);
txphase(zero);
dec2phase(zero);
zgradpulse(gzlvl5, gt5);
if (TROSY[A]=='y') delay(lambda - 0.65*(pw + pwN) - gt5);
else delay(lambda - 1.3*pwN - gt5);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
txphase(one);
dec2phase(t11);
delay(lambda - 1.3*pwN - gt5);
sim3pulse(pw, 0.0, pwN, one, zero, t11, 0.0, 0.0);
txphase(zero);
dec2phase(zero);
zgradpulse(gzlvl6, gt5);
delay(lambda - 1.3*pwN - gt5);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
dec2phase(t10);
zgradpulse(gzlvl6, gt5);
if (TROSY[A]=='y') delay(lambda - 1.6*pwN - gt5);
else delay(lambda - 0.65*pwN - gt5);
if (TROSY[A]=='y') dec2rgpulse(pwN, t10, 0.0, 0.0);
else rgpulse(pw, zero, 0.0, 0.0);
delay((gt1/10.0) + 1.0e-4 +gstab - 0.5*pw + 2.0*GRADIENT_DELAY + POWER_DELAY);
rgpulse(2.0*pw, zero, 0.0,0.0);
dec2power(dpwr2); /* POWER_DELAY */
if (mag_flg[A] == 'y') magradpulse(gzcal*gzlvl2, gt1/10.0);
else zgradpulse(gzlvl2, gt1/10.0); /* 2.0*GRADIENT_DELAY */
delay(gstab);
rcvron();
statusdelay(C,1.0e-4 );
setreceiver(t12);
}
pulsesequence()
{
char N15edit[MAXSTR], C13edit[MAXSTR]; /* C13 editing*/
double tpwrs,pwC,d2,tau,d3,d4,d5,d6,
gt2,gt3,gt0,gzlvl0,gzlvl2,gzlvl3,phincr1,tpwrsf_u,tpwrsf_d,pwHs,compH,
pwN,pwNlvl,ref_pwr,ref_pw90,pwZa,pwClvl,JXH;
pwC=getval("pwC");
pwClvl=getval("pwClvl");
ref_pw90=getval("ref_pw90");
ref_pwr=getval("ref_pwr");
pwHs=getval("pwHs");
gt2=getval("gt2");
gt3=getval("gt3");
gt0=getval("gt0");
/* tau=getval("tau"); */
d2=getval("d2");
d3=getval("d3");
d4=getval("d4");
d5=getval("d5");
gzlvl2=getval("gzlvl2");
gzlvl3=getval("gzlvl3");
gzlvl0=getval("gzlvl0");
phincr1 = getval("phincr1");
d6=getval("d6");
JXH = getval("JXH");
tpwrsf_u = getval("tpwrsf_u"); /* fine power adjustment */
tpwrsf_d = getval("tpwrsf_d"); /* fine power adjustment */
pwHs = getval("pwHs"); /* H1 90 degree pulse length at tpwrs2 */
compH = getval("compH");
pwNlvl = getval("pwNlvl"); /* power for N15 pulses */
pwN = getval("pwN"); /* N15 90 degree pulse length at pwNlvl */
getstr("N15edit",N15edit);
getstr("C13edit",C13edit);
pwZa=pw; /* initialize variable */
/* optional editing for C13 enriched samples */
if ((N15edit[A]=='y') && (C13edit[A]=='n'))
{
pwC = 0.0;
if (2.0*pw > 2.0*pwN) pwZa = pw;
else pwZa = pwN;
}
if ((C13edit[A]=='y')&& (N15edit[A]=='n'))
{
pwN = 0.0;
if (2.0*pw > 2.0*pwC) pwZa = pw;
else pwZa = pwC;
}
if ((C13edit[A]=='y') && (N15edit[A]=='y'))
{
if (2.0*pw > 2.0*pwN) pwZa = pw; /*pwN always longer than pwC*/
else pwZa = pwN;
}
tau = 1/(2*(JXH));
printf("tau is %f\n",tau);
printf("pwZa is %f\n",pwZa);
/* set pwZa to either pw or pwX depending on which is the largest (for calculating delays) */
/*calculate phase cycle for WATERGATE*/
hlv(ct,v1);
hlv(v1,v2);
mod2(v2,v3);
dbl(v3,v4);
assign(two,v5);
add(v4,v5,v6);
obsstepsize(1.0);
if (phincr1 < 0.0) phincr1=360+phincr1;
initval(phincr1,v7);
settable(t1,16,ph1);
settable(t2,16,ph2);
settable(t3,16,ph3);
settable(t4,16,ph4);
settable(t5,16,ph5);
settable(t6,16,ph6);
settable(t7,16,ph7);
settable(t8,16,ph8);
settable(t9,16,ph9);
settable(t10,2,ph10);
tpwrs=tpwr-20.0*log10(pwHs/(compH*pw*1.69)); /* sinc pulse */
tpwrs = (int) (tpwrs) +6.0; /* to permit finepower ~2048 */
/* START THE PULSE SEQUENCE */
status(A);
decpower(pwClvl);
delay(d1);
obsoffset(tof);
/*zgradpulse(gzlvl2,gt2);
delay(d3+d5-pwHs);*/
obspower(tpwrs); obspwrf(tpwrsf_d);
shaped_pulse("H2Osinc_d",pwHs,t2,rof1,rof1);
status(B);
obspower(tpwr); obspwrf(4095.0);
rgpulse(pw,t1,3.0e-6,0.0);
delay(d2);
zgradpulse(gzlvl2,gt2);
delay(d5);
rgpulse(pw,t3,3.0e-6,3.0e-6);
obspower(tpwrs); obspwrf(tpwrsf_u);
shaped_pulse("H2Osinc_u",pwHs,t4,rof1,rof1);
zgradpulse(gzlvl3,gt3);
delay(d3); obspwrf(tpwrsf_d);
shaped_pulse("H2Osinc_d",pwHs,t6,rof1,rof1);
obspower(tpwr); obspwrf(4095.0);
rgpulse(pw,t5,3.0e-6,3.0e-6);
delay(d2);
zgradpulse(gzlvl2,gt2);
delay(d5);
delay(d6);
zgradpulse(gzlvl0,gt0);
obspower(tpwrs);
obspwrf(tpwrsf_d);
xmtrphase(v7);
delay(tau-pwHs-pwZa-gt0-d6-pwN);
shaped_pulse("H2Osinc_d",pwHs,v6,rof1,rof1);
obspower(tpwr);
obspwrf(4095.0);
xmtrphase(zero);
dec2power(pwNlvl);
dec2rgpulse(pwN,zero,1.0e-6,1.0e-6);
decrgpulse(pwC,zero,1.0e-6,1.0e-6);
rgpulse(2*pw,t7,1.0e-6, 1.0e-6);
decrgpulse(pwC,t10,1.0e-6,1.0e-6);
dec2rgpulse(pwN,t10,1.0e-6,1.0e-6);
obspower(tpwrs);
obspwrf(tpwrsf_u);
shaped_pulse("H2Osinc_u",pwHs,t9,rof1,rof1);
delay(tau-pwHs-pwZa-gt0-d6-pwN);
zgradpulse(gzlvl0,gt0);
dec2power(dpwr2);
decpower(dpwr);
delay(d6);
setreceiver(t8);
status(C);
}
void pulsesequence()
{
double mixN = getval("mixN"),
mixNcorr,
gzlvl1 = getval("gzlvl1"),
gt1 = getval("gt1"),
gstab = getval("gstab"),
zqfpw1 = getval("zqfpw1"),
zqfpwr1 = getval("zqfpwr1"),
gzlvlzq1 = getval("gzlvlzq1");
char satmode[MAXSTR],
zqfpat1[MAXSTR],
wet[MAXSTR];
int phase1 = (int)(getval("phase")+0.5);
/* LOAD VARIABLES */
getstr("satmode",satmode);
getstr("wet",wet);
getstr("zqfpat1",zqfpat1);
mixNcorr = 0.0;
if (getflag("PFGflg"))
{
mixNcorr = gt1 + gstab;
if (getflag("Gzqfilt"))
mixNcorr += gstab + zqfpw1;
if (wet[1] == 'y')
mixNcorr += 4*(getval("pwwet")+getval("gtw")+getval("gswet"));
}
if (mixNcorr > mixN)
mixN=mixNcorr;
settable(t1,16,phs1);
settable(t2,32,phs2);
settable(t3,16,phs3);
settable(t5,16,phs5);
settable(t4,32,phs4);
getelem(t1,ct,v1);
getelem(t4,ct,oph);
assign(oph,v7);
assign(zero,v6);
getelem(t2,ct,v2);
getelem(t3,ct,v3);
/*
mod2(id2,v14);
dbl(v14,v14);
*/
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v14);
if (phase1 == 2) /* hypercomplex */
incr(v1);
add(v1, v14, v1);
add(oph,v14,oph);
/* BEGIN THE ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
if (satmode[0] == 'y')
{
if ((d1-satdly) > 0.02)
delay(d1-satdly);
else
delay(0.02);
satpulse(satdly,v6,rof1,rof1);
}
else
delay(d1);
if (wet[0] == 'y')
wet4(zero,one);
status(B);
rgpulse(pw, v1, rof1, rof1);
if (d2>0)
delay(d2- 2*rof1 -(4*pw/PI)); /*corrected evolution time */
else
delay(d2);
rgpulse(pw, v2, rof1, rof1);
if (satmode[1] == 'y')
satpulse((mixN-mixNcorr)*0.7,v7,rof1,rof1);
else
delay((mixN - mixNcorr)*0.7);
if (getflag("PFGflg"))
{
if (getflag("Gzqfilt"))
{
obspower(zqfpwr1);
rgradient('z',gzlvlzq1);
delay(100.0e-6);
shaped_pulse(zqfpat1,zqfpw1,zero,rof1,rof1);
delay(100.0e-6);
rgradient('z',0.0);
delay(gstab);
obspower(tpwr);
}
zgradpulse(gzlvl1,gt1);
delay(gstab);
}
if (satmode[1] == 'y')
satpulse((mixN-mixNcorr)*0.3,v7,rof1,rof1);
else
delay((mixN - mixNcorr)*0.3);
if (wet[1] == 'y')
wet4(zero,one);
status(C);
rgpulse(pw, v3, rof1, rof2);
}
void pulsesequence()
{
/* DECLARE VARIABLES */
char
URA[MAXSTR], /* Setup for U-imino - U-H6 */
flipback[MAXSTR],
CCdseq[MAXSTR],
CYT[MAXSTR], /* Setup for C-imino - C-H6 */
CChomodec[MAXSTR], /* Setup for C-imino - C-H6 */
C5[MAXSTR], /* Setup for C-imino - C-H6 */
C6[MAXSTR], /* Setup for C-imino - C-H6 */
CT[MAXSTR], /* constant time in t1 */
N15refoc[MAXSTR], /* N15 pulse in middle of t1*/
f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR]; /* Flag to start t1 @ halfdwell */
int ni2 = getval("ni2"),
t1_counter,
t2_counter;
double
CCdpwr = getval("CCdpwr"), /* power level for CC decoupling */
CCdres = getval("CCdres"), /* dres for CC decoupling */
CCdmf = getval("CCdmf"), /* dmf for CC decoupling */
tau1, /* t1 delay */
tau2, /* t2 delay */
lambda = 0.94/(4.0*getval("JCH")), /* 1/4J C-H INEPT delay */
lambdaN = 0.94/(4.0*getval("JNH")), /* 1/4J N-H INEPT delay */
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rfC, /* maximum fine power when using pwC pulses */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
rfN, /* maximum fine power when using pwN pulses */
compN = getval("compN"), /* adjustment for N15 amplifier compression */
tpwr = getval("tpwr"), /* power for H1 pulses */
pw = getval("pw"), /* H1 90 degree pulse length at tpwr */
rfH, /* maximum fine power when using pw pulses */
compH = getval("compH"), /* adjustment for H1 amplifier compression */
tof_75, /* tof shifted to 7.5 ppm for H4-N4 transfer */
tof_65, /* tof shifted to 6.0 ppm for H4-N4 transfer */
tof_125, /* tof shifted to 12 ppm for H3-N3 transfer */
dof_169, /* dof shifted to 169 ppm for N3-C4 transfer */
dof_140, /* dof shifted to 140 ppm for C4-C5-C6 transfer and DEC1 */
dof_104, /* dof shifted to 104 ppm for C4-C5-C6 transfer and DEC1 */
dof_153, /* dof shifted to 153 ppm for C4-C5-C6 transfer and DEC1 */
dof_135, /* dof shifted to 135 ppm for C4-C5-C6 transfer and DEC1 */
dof_120, /* dof shifted to 120 ppm for C4-C5-C6 transfer and DEC1 */
dof_130, /* dof shifted to 130 ppm for C4-C5-C6 transfer and DEC1 */
dof_141, /* dof shifted to 141 ppm for C4-C5-C6 transfer and DEC1 */
dof_133, /* dof shifted to 132.5 ppm for C4-C5-C6 transfer and DEC1 */
dof_123, /* dof shifted to 122.5 ppm for C4-C5-C6 transfer and DEC1 */
dof_98, /* dof shifted to 98.0 ppm for C4-C5-C6 transfer and DEC1 */
dof_175, /* dof shifted to 175 ppm for C4-C5-C6 transfer and DEC1 */
dof2_98, /* dof2 shifted to 98.5 ppm for H4-N4 and N4-C4 transfer */
dof2_160, /* dof2 shifted to 160 ppm for H3-N3 and N3-C4 transfer */
/* p_d is used to calculate the isotropic mixing */
p_d, /* 50 degree pulse for DIPSI-3 at rfdC-rfdN-rfdH */
pwZa, /* the largest of 2.0*pw and 2.0*pwN */
rfdC, /* fine C13 power for 1.9 kHz rf for 500MHz magnet */
p_d2, /* 50 degree pulse for DIPSI-3 at rfdC3 */
rfdC3, /* fine C13 power for 10 kHz rf for 500MHz magnet */
rfdN, /* fine N15 power for 1.9 kHz rf for 500MHz magnet */
rfdH, /* fine H1 power for 1.9 kHz rf for 500MHz magnet */
ncyc_hn = getval("ncyc_hn"), /* number of pulsed cycles in HN half-DIPSI-3 */
ncyc_nc = getval("ncyc_nc"), /* number of pulsed cycles in NC DIPSI-3 */
ncyc_cc = getval("ncyc_cc"), /* number of pulsed cycles in CC DIPSI-3 */
CTdelay = getval("CTdelay"), /* total constant time evolution */
sw1 = getval("sw1"),
sw2 = getval("sw2"),
gstab = getval("gstab"),
finepwrf = getval("finepwrf"), /* fine power adjustment */
pwHs = getval("pwHs"), /* H1 90 degree pulse length at tpwrs */
tpwrs, /* power for the pwHs ("rna_H2Osinc") pulse */
pwHs2 = getval("pwHs2"), /* H1 90 degree pulse length at tpwrs2 */
tpwrs2, /* power for the pwHs2 square pulse */
gzlvl0 = getval("gzlvl0"),
gzlvl3 = getval("gzlvl3"),
gt3 = getval("gt3"),
gzlvl4 = getval("gzlvl4"),
gt4 = getval("gt4"),
gzlvl5 = getval("gzlvl5"),
gt5 = getval("gt5"),
gzlvlr = getval("gzlvlr");
getstr("URA",URA);
getstr("flipback",flipback);
getstr("CYT",CYT);
getstr("C5",C5);
getstr("C6",C6);
getstr("CT",CT);
getstr("N15refoc",N15refoc);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("CCdseq",CCdseq);
getstr("CChomodec",CChomodec);
/* LOAD PHASE TABLE */
/*
static int phi1[2] = {0,2},
phi3[8] = {0,0,0,0, 2,2,2,2},
phi4[16]= {0,0,0,0, 0,0,0,0, 2,2,2,2, 2,2,2,2},
phi5[4] = {0,0,2,2},
rec2[8] = {0,2,2,0, 2,0,0,2};
*/
settable(t1,2,phi1);
settable(t3,8,phi3);
settable(t4,16,phi4);
settable(t5,4,phi5);
settable(t10,8,rec2);
/* INITIALIZE VARIABLES */
if (2.0*pw > 2.0*pwN) pwZa = 2.0*pw;
else pwZa = 2.0*pwN;
/* maximum fine power for pwC pulses */
rfC = 4095.0;
/* maximum fine power for pwN pulses */
rfN = 4095.0;
/* maximum fine power for pw pulses */
rfH = 4095.0;
/* different offset values tof=H2O, dof=110ppm, dof2=200ppm */
/* For U 10-15 ppm in Imino region during acquisition (ie 12.5 +/- 2.5 ppm)
and 4.5 -9 ppm during indirect dimensional acquisition (ie 6.75 +/- 2.25 ppm)
For C 4.5 -9ppm (6.75 +/- 2.25ppm) during indirect acqusisition and 6-9ppm during direct (7.5 +/- 1.5ppm)
*/
tof_65 = tof + 2.05*sfrq; /* tof shifted to nH2/nH */
tof_75 = tof + 2.5*sfrq; /* tof shifted to nH2 */
tof_125 = tof + 7.8*sfrq; /* tof shifted to nH */
dof_175 = dof + 65*dfrq; /* dof shifted to C4 */
dof_169 = dof + 59*dfrq; /* dof shifted to C4 */
dof_140 = dof + 30*dfrq; /* dof shifted to C6 */
dof_104 = dof - 6.0*dfrq; /* dof shifted to C6 */
dof_141 = dof + 31*dfrq; /* dof shifted to C6 */
dof_153 = dof + 43*dfrq; /* dof shifted to C6 */
dof_135 = dof + 25*dfrq; /* dof shifted to C6 */
dof_133 = dof + 22.5*dfrq; /* dof shifted to C6 */
dof_120 = dof + 10*dfrq; /* dof shifted to C6 */
dof_130 = dof + 20*dfrq; /* dof shifted to C6 */
dof_98 = dof - 12*dfrq; /* dof shifted to C6 */
dof_123 = dof + 12.5*dfrq; /* dof shifted to C6 */
dof2_160 = dof2 - 40*dfrq2; /* dof2 shifted to Nh */
dof2_98 = dof2 - 101.5*dfrq2; /* dof2 shifted to Nh2 */
/* 1.9 kHz field strength DIPSI-3 at 500MHz adjusted for this sfrq*/
p_d = (5.0)/(9.0*4.0*1900.0*(sfrq/500.0));
/* fine C13 power for dipsi-3 isotropic mixing on C4 region */
rfdC = (compC*4095.0*pwC*5.0)/(p_d*9.0);
rfdC = (int) (rfdC + 0.5);
/* 10 kHz field strength DIPSI-3 at 500MHz adjusted for this sfrq*/
p_d2 = (5.0)/(9.0*4.0*10000.0*(sfrq/500.0));
/* fine C13 power for dipsi-3 isotropic mixing on C2/C6 region */
rfdC3 = (compC*4095.0*pwC*5.0)/(p_d2*9.0);
rfdC3 = (int) (rfdC3 + 0.5);
/* fine N15 power for dipsi-3 isotropic mixing on Nh region */
rfdN = (compN*4095.0*pwN*5.0)/(p_d*9.0);
rfdN = (int) (rfdN + 0.5);
/* fine H1 power for half dipsi-3 isotropic mixing on nH2 region */
rfdH = (compH*4095.0*pw*5.0)/(p_d*9.0);
rfdH = (int) (rfdH + 0.5);
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /* needs 1.69 times more */
tpwrs = (int) (tpwrs); /* power than a square pulse */
/* selective H20 square pulse */
tpwrs2 = tpwr - 20.0*log10(pwHs2/(compH*pw));
tpwrs2 = (int) (tpwrs2);
/* number of cycles and mixing time */
ncyc_nc = (int) (ncyc_nc + 0.5);
ncyc_hn = (int) (ncyc_hn + 0.5);
ncyc_cc = (int) (ncyc_cc + 0.5);
if (ncyc_nc > 0 )
{
printf("NC-mixing time is %f ms.\n",(ncyc_nc*51.8*4*p_d));
}
if (ncyc_cc > 0 )
{
printf("CC-mixing time is %f s.\n",(ncyc_cc*51.8*4*p_d2));
}
/* PHASES AND INCREMENTED TIMES */
/* Phase incrementation for hypercomplex 2D data, States-Haberkorn element */
if (phase1 == 2)
tsadd(t5,1,4);
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{ tsadd(t5,2,4); tsadd(t10,2,4); }
/* Set up f1180 */
tau1 = d2;
if((f1180[A] == 'y') && (ni > 1.0))
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
tau1 = tau1/2.0;
if (phase2 == 2)
{
tsadd(t3,1,4);
}
/* Set up f2180 */
tau2 = d3;
if((f2180[A] == 'y') && (ni2 > 1.0))
{
tau2 += ( 1.0 / (2.0*sw2) );
if(tau2 < 0.2e-6) tau2 = 0.0;
}
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d3_init = d3;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2)
{
tsadd(t3,2,4);
tsadd(t10,2,4);
}
/* CHECK VALIDITY OF PARAMETER RANGE */
if ((CT[A]=='y') && (ni/sw1 > CTdelay))
{ text_error( " ni is too big. Make ni equal to %d or less.\n",
((int)(CTdelay*sw1)) ); psg_abort(1); }
if( sfrq > 610 )
{ printf("Power Levels at 750/800 MHz may be too high for probe");
psg_abort(1); }
if( dpwrf < 4095 )
{ printf("reset dpwrf=4095 and recalibrate C13 90 degree pulse");
psg_abort(1); }
if( dpwrf2 < 4095 )
{ printf("reset dpwrf2=4095 and recalibrate N15 90 degree pulse");
psg_abort(1); }
if((dm[A] == 'y' || dm[B] == 'y'))
{
printf("incorrect dec1 decoupler flag! Should be 'nny' or 'nnn' ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y'))
{
printf("incorrect dec2 decoupler flags! Should be 'nnn' or 'nny' ");
psg_abort(1);
}
if( ((dm[C] == 'y') && (dm2[C] == 'y') && (at > 0.18)) )
{
text_error("check at time! Don't fry probe !! ");
psg_abort(1);
}
if( dpwr > 50 )
{
printf("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if( dpwr2 > 50 )
{
printf("don't fry the probe, DPWR2 too large! ");
psg_abort(1);
}
if( pw > 20.0e-6 )
{
printf("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwC > 40.0e-6 )
{
printf("dont fry the probe, pwC too high ! ");
psg_abort(1);
}
if( pwN > 100.0e-6 )
{
printf("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
if (gzlvlr > 500 || gzlvlr < -500)
{
text_error(" RDt1-gzlvlr must be -500 to 500 (0.5G/cm) \n");
psg_abort(1);
}
if( ncyc_hn > 2 )
{
text_error("check H->N half-dipsi-3 time !! ");
psg_abort(1);
}
if( ncyc_nc > 7 )
{
text_error("check N->C dipsi-3 time !! ");
psg_abort(1);
}
if( ncyc_cc > 7 )
{
text_error("check C->C dipsi-3 time !! ");
psg_abort(1);
}
if((C5[A] == 'y') && ( ncyc_cc > 4) )
{
text_error("check C->C dipsi-3 time equal to 6.5 ms !! ");
psg_abort(1);
}
if((C6[A] == 'y') && (( ncyc_cc > 6) || ( ncyc_cc < 3)))
{
text_error("check C->C dipsi-3 time equal to 13 ms !! ");
psg_abort(1);
}
if( (URA[A] == 'y') && (CYT[A] == 'y') )
{
text_error("Choose either URA or CYT !! ");
psg_abort(1);
}
if( (URA[A] == 'n') && (CYT[A] == 'n') )
{
text_error("Do you really want to run this experiment ?? ");
psg_abort(1);
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
obspwrf(rfH);
obsstepsize(0.5);
decpower(pwClvl);
decpwrf(rfC);
decstepsize(0.5);
dec2power(pwNlvl);
dec2pwrf(rfN);
dec2stepsize(0.5);
if (C6[A]=='y') decoffset(dof_141); /* frequency for the NC-tocsy */
if (URA[A] == 'y')
{
obsoffset(tof_65); /* Set the proton frequency to U-nH */
dec2offset(dof2_160); /* Set the nitrogen frequency to U-Nh */
if (C5[A]=='y') decoffset(dof_104);
}
else if (CYT[A] == 'y')
{
obsoffset(tof_65); /* Set the proton frequency to C-nH2 */
dec2offset(dof2_98); /* Set the nitrogen frequency to C-Nh2 */
if (C5[A]=='y') decoffset(dof_98);
}
else
{
}
txphase(zero);
decphase(zero);
dec2phase(zero);
delay(d1);
rcvroff();
dec2rgpulse(pwN, zero, 0.0, 0.0); /*destroy N15 and C13 magnetization*/
decrgpulse(pwC, zero, 0.0, 0.0);
zgradpulse(gzlvl0, 0.5e-3);
delay(1.0e-4);
dec2rgpulse(pwN, one, 0.0, 0.0);
decrgpulse(pwC, one, 0.0, 0.0);
zgradpulse(0.7*gzlvl0, 0.5e-3);
delay(5.0e-4);
initval(ncyc_nc,v11);
initval(ncyc_cc,v2);
txphase(t1);
decphase(zero);
dec2phase(zero);
delay(5.0e-4);
rcvroff();
rgpulse(pw, t1, 50.0e-6, 0.0); /* x,-x */
txphase(zero);
delay(lambda);
simpulse(2*pw, 2*pwC, zero, zero, 0.0, 0.0);
decphase(t5);
delay(lambda);
simpulse(pw, pwC, one, t5, 0.0, 0.0); /* x, -x */
decphase(zero);
zgradpulse(gzlvl5,gt5);
delay(lambda - gt5);
simpulse(2*pw, 2*pwC, one, zero, 0.0, 0.0);
zgradpulse(gzlvl5,gt5);
delay(lambda - gt5 - 2*POWER_DELAY);
if (CChomodec[A]=='y')
{
decpower(CCdpwr); decphase(zero);
decprgon(CCdseq,1.0/CCdmf,CCdres);
decon(); /* CC decoupling on */
if (N15refoc[A]=='y')
{
if (tau1 > (pwN + 0.64*pw))
{
delay(tau1 - pwN - 0.64*pw);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
delay(tau1 - pwN - 0.64*pw);
}
else if (tau1 > 0.64*pw)
delay(2.0*tau1 - 2.0*0.64*pw);
}
else
{
if (tau1 > pw)
{
delay(tau1 - 0.64*pw);
rgpulse(2.0*pw, zero, 0.0, 0.0);
delay(tau1 - 0.64*pw);
}
else
delay(2.0*tau1);
}
decoff(); decprgoff(); /* CC decoupling off */
decpower(pwClvl);
} /* END CC H**O DEC */
else
{
/***************** CONSTANT TIME EVOLUTION *****************/
if (CT[A]=='y') {
/***************/
delay(CTdelay/2.0 - tau1);
decrgpulse(2.0*pwC, zero, 2.0e-6, 0.0);
{delay(CTdelay/2.0 - pwZa);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);}
delay(tau1);
/***************/
}
else if (N15refoc[A]=='y')
{
if (tau1 > (2.0*GRADIENT_DELAY + pwN + 0.64*pw + 5.0*SAPS_DELAY))
{
zgradpulse(gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw) - SAPS_DELAY);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(-1.0*gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
}
else if (tau1 > (0.64*pw + 0.5*SAPS_DELAY))
delay(2.0*tau1 - 2.0*0.64*pw - SAPS_DELAY );
}
else
{
if (tau1 > (2.0*GRADIENT_DELAY + pw + 5.0*SAPS_DELAY))
{
zgradpulse(gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw) - SAPS_DELAY);
rgpulse(2.0*pw, zero, 0.0, 0.0);
zgradpulse(-1.0*gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
}
else if (tau1 > (pw + 0.5*SAPS_DELAY))
delay(2.0*tau1 - 2.0*pw - SAPS_DELAY );
}
} /* End No CC homodec */
decrgpulse(pwC,one,0.0,0.0); /* flip transferred 13C-magnetization to x */
decoffset(dof_135); /* frequency for the NC-tocsy */
decrgpulse(pwC,three,0.0,0.0); /* flip transferred 13C-magnetization to x */
decphase(zero);
decpwrf(rfdC3);
starthardloop(v2);
decrgpulse(6.4*p_d2,zero,0.0,0.0);
decrgpulse(8.2*p_d2,two,0.0,0.0);
decrgpulse(5.8*p_d2,zero,0.0,0.0);
decrgpulse(5.7*p_d2,two,0.0,0.0);
decrgpulse(0.6*p_d2,zero,0.0,0.0);
decrgpulse(4.9*p_d2,two,0.0,0.0);
decrgpulse(7.5*p_d2,zero,0.0,0.0);
decrgpulse(5.3*p_d2,two,0.0,0.0);
decrgpulse(7.4*p_d2,zero,0.0,0.0);
decrgpulse(6.4*p_d2,two,0.0,0.0);
decrgpulse(8.2*p_d2,zero,0.0,0.0);
decrgpulse(5.8*p_d2,two,0.0,0.0);
decrgpulse(5.7*p_d2,zero,0.0,0.0);
decrgpulse(0.6*p_d2,two,0.0,0.0);
decrgpulse(4.9*p_d2,zero,0.0,0.0);
decrgpulse(7.5*p_d2,two,0.0,0.0);
decrgpulse(5.3*p_d2,zero,0.0,0.0);
decrgpulse(7.4*p_d2,two,0.0,0.0);
decrgpulse(6.4*p_d2,two,0.0,0.0);
decrgpulse(8.2*p_d2,zero,0.0,0.0);
decrgpulse(5.8*p_d2,two,0.0,0.0);
decrgpulse(5.7*p_d2,zero,0.0,0.0);
decrgpulse(0.6*p_d2,two,0.0,0.0);
decrgpulse(4.9*p_d2,zero,0.0,0.0);
decrgpulse(7.5*p_d2,two,0.0,0.0);
decrgpulse(5.3*p_d2,zero,0.0,0.0);
decrgpulse(7.4*p_d2,two,0.0,0.0);
decrgpulse(6.4*p_d2,zero,0.0,0.0);
decrgpulse(8.2*p_d2,two,0.0,0.0);
decrgpulse(5.8*p_d2,zero,0.0,0.0);
decrgpulse(5.7*p_d2,two,0.0,0.0);
decrgpulse(0.6*p_d2,zero,0.0,0.0);
decrgpulse(4.9*p_d2,two,0.0,0.0);
decrgpulse(7.5*p_d2,zero,0.0,0.0);
decrgpulse(5.3*p_d2,two,0.0,0.0);
decrgpulse(7.4*p_d2,zero,0.0,0.0);
endhardloop();
decphase(one);
decpwrf(rfC);
decrgpulse(pwC,three,0.0,0.0); /* flip transferred 13C-magnetization to x */
decoffset(dof_175);
decrgpulse(pwC,one,0.0,0.0); /* flip transferred 13C-magnetization to x */
decpwrf(rfdC); /* Set fine power for carbon */
dec2pwrf(rfdN); /* Set fine power for nitrogen */
dec2phase(zero);
decphase(zero);
starthardloop(v11);
sim3pulse(0.0,6.4*p_d,6.4*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,8.2*p_d,8.2*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,5.8*p_d,5.8*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,5.7*p_d,5.7*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,0.6*p_d,0.6*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,4.9*p_d,4.9*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,7.5*p_d,7.5*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,5.3*p_d,5.3*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,7.4*p_d,7.4*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,6.4*p_d,6.4*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,8.2*p_d,8.2*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,5.8*p_d,5.8*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,5.7*p_d,5.7*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,0.6*p_d,0.6*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,4.9*p_d,4.9*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,7.5*p_d,7.5*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,5.3*p_d,5.3*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,7.4*p_d,7.4*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,6.4*p_d,6.4*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,8.2*p_d,8.2*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,5.8*p_d,5.8*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,5.7*p_d,5.7*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,0.6*p_d,0.6*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,4.9*p_d,4.9*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,7.5*p_d,7.5*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,5.3*p_d,5.3*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,7.4*p_d,7.4*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,6.4*p_d,6.4*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,8.2*p_d,8.2*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,5.8*p_d,5.8*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,5.7*p_d,5.7*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,0.6*p_d,0.6*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,4.9*p_d,4.9*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,7.5*p_d,7.5*p_d,zero,zero,zero,0.0,0.0);
sim3pulse(0.0,5.3*p_d,5.3*p_d,two,two,two,0.0,0.0);
sim3pulse(0.0,7.4*p_d,7.4*p_d,zero,zero,zero,0.0,0.0);
endhardloop();
obspwrf(rfH);
decpwrf(rfC);
dec2pwrf(rfN);
obsoffset(tof);
txphase(zero);
decphase(one);
if (tau2 > 0.0)
{
if (tau2 > (2.0*GRADIENT_DELAY + pwC + 0.64*pw + 5.0*SAPS_DELAY))
{
zgradpulse(gzlvlr, 0.8*(tau2 - 2.0*GRADIENT_DELAY - pwC - 0.64*pw));
delay(0.2*(tau2 - 2.0*GRADIENT_DELAY - pwC - 0.64*pw) - SAPS_DELAY);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(-1.0*gzlvlr, 0.8*(tau2 - 2.0*GRADIENT_DELAY - pwC - 0.64*pw));
delay(0.2*(tau2 - 2.0*GRADIENT_DELAY - pwC - 0.64*pw));
}
else if (tau2 > (0.64*pw + 0.5*SAPS_DELAY))
delay(2.0*tau2 - 2.0*0.64*pw - SAPS_DELAY );
}
else
{;}
if( CYT[A] == 'y' )
{
zgradpulse(gzlvl5,gt5);
delay(lambdaN/2.0 - gt5);
sim3pulse(2*pw, 0.0, 2*pwN,zero, zero,zero,0.0,0.0);
zgradpulse(gzlvl5,gt5);
delay(lambdaN/2.0 - gt5);
}
else if( URA[A] == 'y' )
{
zgradpulse(gzlvl5,gt5);
delay(lambdaN - gt5);
sim3pulse(2*pw, 0.0, 2*pwN,zero, zero,zero,0.0,0.0);
zgradpulse(gzlvl5,gt5);
delay(lambdaN - gt5);
}
dec2rgpulse(pwN,t3,0.0,0.0);
if (flipback[A]=='y')
{
zgradpulse(gzlvl3,gt3);
delay(gstab);
txphase(zero);
obspower(tpwrs);
shaped_pulse("rna_H2Osinc", pwHs, zero, 5.0e-4, 0.0);
obspower(tpwr);
}
rgpulse(pw, zero, 2*rof1, 0.0);
txphase(two);
obspower(tpwrs2);
obspwrf(finepwrf);
zgradpulse(gzlvl4,gt4);
delay(lambdaN - 2.0*POWER_DELAY - gt4 -rof1 -2.0*GRADIENT_DELAY - pwHs2);
rgpulse(pwHs2, two, rof1, rof1);
obspower(tpwr);
obspwrf(4095.0);
sim3pulse(2*pw, 0.0, 2*pwN, zero, zero, zero, rof1, rof1);
obspwrf(finepwrf);
obspower(tpwrs2);
rgpulse(pwHs2, two, rof1, rof1);
zgradpulse(gzlvl4,gt4);
delay(lambdaN - 3*POWER_DELAY - gt4 - 2.0*GRADIENT_DELAY - pwHs2);
dec2rgpulse(pwN,t4,0.0,0.0);
dec2rgpulse(pwN,zero,0.0,0.0);
dec2power(dpwr2); /* 2*POWER_DELAY */
decpower(dpwr);
status(C);
rcvron();
setreceiver(t10);
}
pulsesequence()
{
/* DECLARE AND LOAD VARIABLES */
char f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
mag_flg[MAXSTR], /* magic-angle coherence transfer gradients */
C13refoc[MAXSTR], /* C13 sech/tanh pulse in middle of t1*/
NH2only[MAXSTR], /* spectrum of only NH2 groups */
amino[MAXSTR], /* select amino nitrogens */
imino[MAXSTR], /* select imino nitrogens */
T1[MAXSTR], /* insert T1 relaxation delay */
T1rho[MAXSTR], /* insert T1rho relaxation delay */
T2[MAXSTR], /* insert T2 relaxation delay */
bottom[MAXSTR],
right[MAXSTR],
TROSY[MAXSTR]; /* do TROSY on N15 and H1 */
int icosel, /* used to get n and p type */
t1_counter, /* used for states tppi in t1 */
rTnum, /* number of relaxation times, relaxT */
rTcounter; /* to obtain maximum relaxT, ie relaxTmax */
double tau1, /* t1 delay */
lambda = 0.91/(4.0*getval("JNH")), /* 1/4J H1 evolution delay */
tNH = 1.0/(4.0*getval("JNH")), /* 1/4J N15 evolution delay */
relaxT = getval("relaxT"), /* total relaxation time */
rTarray[1000], /* to obtain maximum relaxT, ie relaxTmax */
maxrelaxT = getval("maxrelaxT"), /* maximum relaxT in all exps */
ncyc, /* number of pulsed cycles in relaxT */
/* the sech/tanh pulse is automatically calculated by the macro "rna_cal", */ /* and is called directly from your shapelib. */
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rfC, /* maximum fine power when using pwC pulses */
rfst, /* fine power for the rna_stC140 pulse */
/* temporary Pbox parameters */
bw, pws, ofs, ppm, nst, /* bandwidth, pulsewidth, offset, ppm, # steps */
compH = getval("compH"), /* adjustment for H1 amplifier compression */
compN = getval("compN"), /* adjustment for N15 amplifier compression */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
calH = getval("calH"), /* multiplier on a pw pulse for H1 calibration */
pwHs = getval("pwHs"), /* H1 90 degree pulse length at tpwrs */
tpwrs, /* power for the pwHs ("H2Osinc") pulse */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
calN = getval("calN"), /* multiplier on a pwN pulse for calibration */
slNlvl, /* power for N15 spin lock */
slNrf = 1500.0, /* RF field in Hz for N15 spin lock at 600 MHz */
dof2a, /* offset for imino/amino */
sw1 = getval("sw1"),
gt1 = getval("gt1"), /* coherence pathway gradients */
gzcal = getval("gzcal"), /* dac to G/cm conversion */
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
BPpwrlimits, /* =0 for no limit, =1 for limit */
gt0 = getval("gt0"), /* other gradients */
gt3 = getval("gt3"),
gt4 = getval("gt4"),
gt5 = getval("gt5"),
gzlvl0 = getval("gzlvl0"),
gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5");
P_getreal(GLOBAL,"BPpwrlimits",&BPpwrlimits,1);
getstr("f1180",f1180);
getstr("mag_flg",mag_flg);
getstr("C13refoc",C13refoc);
getstr("NH2only",NH2only);
getstr("T1",T1);
getstr("T1rho",T1rho);
getstr("T2",T2);
getstr("bottom",bottom);
getstr("right",right);
getstr("TROSY",TROSY);
getstr("imino",imino);
getstr("amino",amino);
/* LOAD PHASE TABLE */
settable(t3,2,phi3);
if (TROSY[A]=='y')
{ settable(t1,1,ph_x);
if (bottom[A]=='y')
settable(t4,1,phx);
else
settable(t4,1,ph_x);
if (right[A]=='y')
settable(t10,1,phy);
else
settable(t10,1,ph_y);
settable(t9,1,phx);
settable(t11,1,phx);
settable(t12,2,recT);
}
else
{ settable(t1,1,phx);
settable(t4,1,phx);
settable(t9,8,phi9);
settable(t10,1,phx);
settable(t11,1,phy);
settable(t12,4,rec);
}
/* INITIALIZE VARIABLES */
dof2a=dof2;
/* IMINO-region setting of dof2 */
if (imino[A] == 'y') dof2a=dof2-45*dfrq2;
if (amino[A] == 'y') dof2a=dof2-115*dfrq2;
if ((imino[A] == 'n') && (amino[A] == 'n')) dof2a=dof2;
/* maximum fine power for pwC pulses (and initialize rfst) */
rfC = 4095.0;
rfst=0.0;
setautocal(); /* activate auto-calibration flags */
if (autocal[0] == 'n')
{
/* 180 degree adiabatic C13 pulse covers 140 ppm */
if (C13refoc[A]=='y')
{ rfst = (compC*4095.0*pwC*4000.0*sqrt((21.0*sfrq/600.0+7.0)/0.35));
rfst = (int) (rfst + 0.5);
if ( 1.0/(4000.0*sqrt((21.0*sfrq/600.0+7.0)/0.35)) < pwC )
{ text_error( " Not enough C13 RF. pwC must be %f usec or less.\n",
(1.0e6/(4000.0*sqrt((21.0*sfrq/600.0+7.0)/0.35))) );
psg_abort(1);
}
}
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /*needs 1.69 times more*/
tpwrs = (int) (tpwrs); /*power than a square pulse */
}
else /* if autocal = 'y'(yes), 'q'(quiet), r(read), or 's'(semi) */
{
if(FIRST_FID) /* call Pbox */
{
ppm = getval("dfrq");
bw = 140.0*ppm;
pws = 0.001;
ofs = 0.0;
nst = 1000.0;
if (C13refoc[A]=='y')
stC140 = pbox_makeA("rna_stC140", "sech", bw, pws, ofs, compC*pwC, pwClvl, nst);
H2Osinc = pbox_Rsh("rna_H2Osinc", "sinc90", pwHs, 0.0, compH*pw, tpwr);
if (dm3[B] == 'y') H2ofs = 3.2;
ofs_check(H1ofs, C13ofs, N15ofs, H2ofs);
}
if (C13refoc[A]=='y') rfst = stC140.pwrf;
pwHs = H2Osinc.pw;
tpwrs = H2Osinc.pwr;
}
/* power level for N15 spinlock (90 degree pulse length calculated first) */
slNlvl = 1/(4.0*slNrf*sfrq/600.0) ;
slNlvl = pwNlvl - 20.0*log10(slNlvl/(pwN*compN));
slNlvl = (int) (slNlvl + 0.5);
/* use 1/8J times for relaxation measurements of NH2 groups */
if ( (NH2only[A]=='y') && ((T1[A]=='y') || (T1rho[A]=='y') || (T2[A]=='y')) )
{
tNH = tNH/2.0;
}
/* reset calH and calN for 2D if inadvertently left at 2.0 */
if (ni>1.0) {
calH=1.0;
calN=1.0;
}
/* CHECK VALIDITY OF PARAMETER RANGES */
if ( ((imino[A] == 'y') && (amino[A] == 'y')) )
{
printf(" Choose ONE of the cases: imino='y' OR amino='y' ");
psg_abort(1);
}
if ( ((imino[A] == 'y') && (NH2only[A] == 'y')) )
{
printf(" NH2only='y' only valide for amino='y' ");
psg_abort(1);
}
if ((TROSY[A]=='y') && (gt1 < -2.0e-4 + pwHs + 1.0e-4 + 2.0*POWER_DELAY))
{ text_error( " gt1 is too small. Make gt1 equal to %f or more.\n",
(-2.0e-4 + pwHs + 1.0e-4 + 2.0*POWER_DELAY) );
psg_abort(1);
}
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y' ))
{
text_error("incorrect dec1 decoupler flags! Should be 'nnn' ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y'))
{
text_error("incorrect dec2 decoupler flags! Should be 'nny' ");
psg_abort(1);
}
if( dpwr2 > 50 )
{
text_error("don't fry the probe, DPWR2 too large! ");
psg_abort(1);
}
if( pw > 50.0e-6 )
{
text_error("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( (pwN > 100.0e-6) && (pwNlvl > 54) )
{
text_error("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
/* RELAXATION TIMES AND FLAGS */
/* evaluate maximum relaxT, relaxTmax chosen by the user */
rTnum = getarray("relaxT", rTarray);
relaxTmax = rTarray[0];
for (rTcounter=1; rTcounter<rTnum; rTcounter++)
if (relaxTmax < rTarray[rTcounter]) relaxTmax = rTarray[rTcounter];
/* compare relaxTmax with maxrelaxT */
if (maxrelaxT > relaxTmax) relaxTmax = maxrelaxT;
if ( ((T1rho[A]=='y') || (T2[A]=='y')) && (relaxTmax > d1) )
{
text_error("Maximum relaxation time, relaxT, is greater than d1 ! ");
psg_abort(1);
}
if ( ((T1[A]=='y') && (T1rho[A]=='y')) || ((T1[A]=='y') && (T2[A]=='y')) ||
((T1rho[A]=='y') && (T2[A]=='y')) )
{
text_error("Choose only one relaxation measurement ! ");
psg_abort(1);
}
if ( ((T1[A]=='y') || (T1rho[A]=='y')) &&
((relaxT*100.0 - (int)(relaxT*100.0+1.0e-4)) > 1.0e-6) )
{
text_error("Relaxation time, relaxT, must be zero or multiple of 10msec");
psg_abort(1);
}
if ( (T2[A]=='y') &&
(((relaxT+0.01)*50.0 - (int)((relaxT+0.01)*50.0+1.0e-4)) > 1.0e-6) )
{
text_error("Relaxation time, relaxT, must be odd multiple of 10msec");
psg_abort(1);
}
if ( ((T1rho[A]=='y') || (T2[A]=='y')) && (relaxTmax > 0.25) && (ix==1) )
{
printf("WARNING, sample heating may result in a reduced lock level for relaxT>0.25sec");
}
if ( ((T1rho[A]=='y') || (T2[A]=='y')) && (relaxTmax > 0.5) )
{
text_error("relaxT greater than 0.5 seconds will heat sample");
psg_abort(1);
}
if ( ((NH2only[A]=='y') || (T1[A]=='y') || (T1rho[A]=='y') || (T2[A]=='y'))
&& (TROSY[A]=='y') )
{
text_error("TROSY not implemented with NH2 spectrum, or relaxation exps.");
psg_abort(1);
}
if ((TROSY[A]=='y') && (dm2[C] == 'y'))
{
text_error("Choose either TROSY='n' or dm2='nnn' ! ");
psg_abort(1);
}
/* PHASES AND INCREMENTED TIMES */
/* Phase incrementation for hypercomplex 2D data, States-Haberkorn element */
if (TROSY[A]=='y')
{ if (phase1 == 1) icosel = -1;
else {
tsadd(t4,2,4);
tsadd(t10,2,4);
icosel = +1;
}
}
else {
if (phase1 == 1) {
tsadd(t10,2,4);
icosel = +1;
}
else icosel = -1;
}
/* Set up f1180 */
tau1 = d2;
if((f1180[A] == 'y') && (ni > 1.0))
{
tau1 += ( 1.0 / (2.0*sw1) );
if(tau1 < 0.2e-6) tau1 = 0.0;
}
tau1 = tau1/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{
tsadd(t3,2,4);
tsadd(t12,2,4);
}
/* Correct inverted signals for NH2 only spectra */
if ((NH2only[A]=='y') && (T1[A]=='n') && (T1rho[A]=='n') && (T2[A]=='n'))
{
tsadd(t3,2,4);
}
/* BEGIN PULSE SEQUENCE */
status(A);
obspower(tpwr);
decpower(pwClvl);
decpwrf(rfC);
dec2power(pwNlvl);
dec2offset(dof2a);
txphase(zero);
decphase(zero);
dec2phase(zero);
delay(d1);
/* xxxxxxxxxxxxxxxxx CONSTANT SAMPLE HEATING FROM N15 RF xxxxxxxxxxxxxxxxx */
if (T1rho[A]=='y')
{ dec2power(slNlvl);
dec2rgpulse(relaxTmax-relaxT, zero, 0.0, 0.0);
dec2power(pwNlvl);
}
if (T2[A]=='y')
{ ncyc = 8.0*100.0*(relaxTmax - relaxT);
if (BPpwrlimits > 0.5)
{
dec2power(pwNlvl-3.0); /* reduce for probe protection */
pwN=pwN*compN*1.4;
}
if (ncyc > 0)
{ initval(ncyc,v1);
loop(v1,v2);
delay(0.625e-3 - pwN);
dec2rgpulse(2*pwN, zero, 0.0, 0.0);
delay(0.625e-3 - pwN);
endloop(v2);
}
if (BPpwrlimits > 0.5)
{
dec2power(pwNlvl); /* restore normal value */
pwN=getval("pwN");
}
}
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
rcvroff();
if (TROSY[A]=='n') dec2rgpulse(pwN, zero, 0.0, 0.0);
decrgpulse(pwC, zero, 0.0, 0.0); /*destroy N15 and C13 magnetization*/
zgradpulse(gzlvl0, 0.5e-3);
delay(1.0e-4);
if (TROSY[A]=='n') dec2rgpulse(pwN, one, 0.0, 0.0);
decrgpulse(pwC, one, 0.0, 0.0);
zgradpulse(0.7*gzlvl0, 0.5e-3);
decpwrf(rfst);
txphase(t1);
delay(5.0e-4);
if(dm3[B] == 'y') /*optional 2H decoupling on */
{ lk_hold();
dec3unblank();
dec3rgpulse(1/dmf3, one, 0.0, 0.0);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
}
rgpulse(calH*pw,t1,0.0,0.0); /* 1H pulse excitation */
txphase(zero);
dec2phase(zero);
zgradpulse(gzlvl0, gt0);
delay(lambda - gt0);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
txphase(one);
zgradpulse(gzlvl0, gt0);
delay(lambda - gt0);
rgpulse(pw, one, 0.0, 0.0);
txphase(two);
obspower(tpwrs);
shaped_pulse("rna_H2Osinc", pwHs, two, 5.0e-5, 0.0);
obspower(tpwr);
zgradpulse(gzlvl3, gt3);
dec2phase(t3);
delay(2.0e-4);
dec2rgpulse(calN*pwN, t3, 0.0, 0.0);
txphase(zero);
decphase(zero);
/* xxxxxxxxxxxxxxxxxx OPTIONS FOR N15 RELAXATION xxxxxxxxxxxxxxxxxxxx */
if ( (T1[A]=='y') || (T1rho[A]=='y') || (T2[A]=='y') )
{
dec2phase(one);
zgradpulse(gzlvl4, gt4); /* 2.0*GRADIENT_DELAY */
delay(tNH - gt4 - 2.0*GRADIENT_DELAY);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, one, 0.0, 0.0);
zgradpulse(gzlvl4, gt4); /* 2.0*GRADIENT_DELAY */
delay(tNH - gt4 - 2.0*GRADIENT_DELAY);
}
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
if (T1[A]=='y')
{
dec2rgpulse(pwN, one, 0.0, 0.0);
dec2phase(three);
zgradpulse(gzlvl0, gt0); /* 2.0*GRADIENT_DELAY */
delay(2.5e-3 - gt0 - 2.0*GRADIENT_DELAY - pw);
rgpulse(2.0*pw, zero, 0.0, 0.0);
delay(2.5e-3 - pw);
ncyc = (100.0*relaxT);
initval(ncyc,v4);
if (ncyc > 0)
{ loop(v4,v5);
delay(2.5e-3 - pw);
rgpulse(2.0*pw, two, 0.0, 0.0);
delay(2.5e-3 - pw);
delay(2.5e-3 - pw);
rgpulse(2.0*pw, zero, 0.0, 0.0);
delay(2.5e-3 - pw);
endloop(v5);
}
dec2rgpulse(pwN, three, 0.0, 0.0);
}
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
/* Theory suggests 8.0 is better than 2PI as RF */
/* field multiplier and experiment confirms this.*/
if (T1rho[A]=='y') /* Shift evolution of 2.0*pwN/PI for one pulse */
{ /* at end left unrefocused as for normal sequence*/
delay(1.0/(8.0*slNrf) - pwN);
decrgpulse(pwN, zero, 0.0, 0.0);
dec2power(slNlvl);
/* minimum 5ms spinlock to dephase */
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0); /* spins not locked */
sim3pulse(2.0*pw, 0.0, 2.0*pw, zero, zero, zero, 0.0, 0.0);
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0);
ncyc = 100.0*relaxT;
initval(ncyc,v4);
if (ncyc > 0)
{ loop(v4,v5);
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0);
sim3pulse(2.0*pw, 0.0, 2.0*pw, two, zero, zero, 0.0, 0.0);
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0);
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0);
sim3pulse(2.0*pw, 0.0, 2.0*pw, zero, zero, zero, 0.0, 0.0);
dec2rgpulse((2.5e-3-pw), zero, 0.0, 0.0);
endloop(v5);
}
dec2power(pwNlvl);
decrgpulse(pwN, zero, 0.0, 0.0);
delay(1.0/(8.0*slNrf) + 2.0*pwN/PI - pwN);
}
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
if (T2[A]=='y')
{
dec2phase(zero);
initval(0.0,v3);
initval(180.0,v4);
if (BPpwrlimits > 0.5)
{
dec2power(pwNlvl-3.0); /* reduce for probe protection */
pwN=pwN*compN*1.4;
}
ncyc = 100.0*relaxT;
initval(ncyc,v5);
loop(v5,v6);
initval(3.0,v7);
loop(v7,v8);
delay(0.625e-3 - pwN);
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
delay(0.625e-3 - pwN);
endloop(v8);
delay(0.625e-3 - pwN - SAPS_DELAY);
add(v4,v3,v3);
obsstepsize(1.0);
xmtrphase(v3); /* SAPS_DELAY */
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
delay(0.625e-3 - pwN - pw);
rgpulse(2*pw, zero, 0.0, 0.0);
delay(0.625e-3 - pwN - pw );
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
xmtrphase(zero); /* SAPS_DELAY */
delay(0.625e-3 - pwN - SAPS_DELAY);
initval(3.0,v9);
loop(v9,v10);
delay(0.625e-3 - pwN);
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
delay(0.625e-3 - pwN);
endloop(v10);
endloop(v6);
if (BPpwrlimits > 0.5)
{
dec2power(pwNlvl); /* restore normal value */
pwN=getval("pwN");
}
}
/* xxxxxxxxxxxxxxxxxx OPTIONS FOR N15 EVOLUTION xxxxxxxxxxxxxxxxxxxxx */
txphase(zero);
dec2phase(t9);
if ( (NH2only[A]=='y') || (T1[A]=='y') || (T1rho[A]=='y') || (T2[A]=='y') )
{
delay(tau1);
/* optional sech/tanh pulse in middle of t1 */
if (C13refoc[A]=='y') /* WFG_START_DELAY */
{ decshaped_pulse("rna_stC140", 1.0e-3, zero, 0.0, 0.0);
delay(tNH - 1.0e-3 - WFG_START_DELAY - 2.0*pw);
}
else
{
delay(tNH - 2.0*pw);
}
rgpulse(2.0*pw, zero, 0.0, 0.0);
if (tNH < gt1 + 1.99e-4) delay(gt1 + 1.99e-4 - tNH);
delay(tau1);
dec2rgpulse(2.0*pwN, t9, 0.0, 0.0);
if (mag_flg[A] == 'y') magradpulse(gzcal*gzlvl1, gt1);
else zgradpulse(gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
txphase(t4);
dec2phase(t10);
if (tNH > gt1 + 1.99e-4) delay(tNH - gt1 - 2.0*GRADIENT_DELAY);
else delay(1.99e-4 - 2.0*GRADIENT_DELAY);
}
else if (TROSY[A]=='y')
{
if ( (C13refoc[A]=='y') && (tau1 > 0.5e-3 + WFG2_START_DELAY) )
{ delay(tau1 - 0.5e-3 - WFG2_START_DELAY); /* WFG2_START_DELAY */
decshaped_pulse("rna_stC140", 1.0e-3, zero, 0.0, 0.0);
delay(tau1 - 0.5e-3);
}
else delay(2.0*tau1);
if (mag_flg[A] == 'y') magradpulse(gzcal*gzlvl1, gt1);
else zgradpulse(gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
delay(2.0e-4 - 2.0*GRADIENT_DELAY);
dec2rgpulse(2.0*pwN, t9, 0.0, 0.0);
delay(gt1 + 2.0e-4 - pwHs - 1.0e-4 - 2.0*POWER_DELAY);
txphase(three);
obspower(tpwrs); /* POWER_DELAY */
shaped_pulse("rna_H2Osinc", pwHs, three, 5.0e-5, 0.0);
txphase(t4);
obspower(tpwr); /* POWER_DELAY */
delay(5.0e-5);
}
else
{ /* fully-coupled spectrum */
if (dm2[C]=='n') {
rgpulse(2.0*pw, zero, 0.0, 0.0);
pw=0.0;
}
if ( (C13refoc[A]=='y') && (tau1 > 0.5e-3 + WFG2_START_DELAY) )
{ delay(tau1 - 0.5e-3 - WFG2_START_DELAY); /* WFG2_START_DELAY */
simshaped_pulse("", "rna_stC140", 2.0*pw, 1.0e-3, zero, zero, 0.0, 0.0);
delay(tau1 - 0.5e-3);
delay(gt1 + 2.0e-4);
}
else
{ delay(tau1);
rgpulse(2.0*pw, zero, 0.0, 0.0);
delay(gt1 + 2.0e-4 - 2.0*pw);
delay(tau1);
}
decphase(zero);
pw=getval("pw");
dec2rgpulse(2.0*pwN, t9, 0.0, 0.0);
if (mag_flg[A] == 'y') magradpulse(gzcal*gzlvl1, gt1);
else zgradpulse(gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
txphase(t4);
dec2phase(t10);
delay(2.0e-4 - 2.0*GRADIENT_DELAY);
}
if (T1rho[A]=='y') delay(POWER_DELAY);
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
if (TROSY[A]=='y') rgpulse(pw, t4, 0.0, 0.0);
else sim3pulse(pw, 0.0, pwN, t4, zero, t10, 0.0, 0.0);
txphase(zero);
dec2phase(zero);
zgradpulse(gzlvl5, gt5);
if (TROSY[A]=='y') delay(lambda - 0.65*(pw + pwN) - gt5);
else delay(lambda - 1.3*pwN - gt5);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
txphase(one);
dec2phase(t11);
delay(lambda - 1.3*pwN - gt5);
sim3pulse(pw, 0.0, pwN, one, zero, t11, 0.0, 0.0);
txphase(zero);
dec2phase(zero);
zgradpulse(1.5*gzlvl5, gt5);
delay(lambda - 1.3*pwN - gt5);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
dec2phase(t10);
zgradpulse(1.5*gzlvl5, gt5);
if (TROSY[A]=='y') delay(lambda - 1.6*pwN - gt5);
else delay(lambda - 0.65*pwN - gt5);
if (TROSY[A]=='y') dec2rgpulse(pwN, t10, 0.0, 0.0);
else rgpulse(pw, zero, 0.0, 0.0);
delay((gt1/10.0) + 1.0e-4 - 0.65*pw + 2.0*GRADIENT_DELAY + POWER_DELAY);
if (dm3[B] == 'y') /*optional 2H decoupling off */
{ dec3rgpulse(1/dmf3, three, 0.0, 0.0);
dec3blank();
setstatus(DEC3ch, FALSE, 'w', FALSE, dmf3);
dec3blank();
}
rgpulse(2.0*pw, zero, 0.0, rof1);
dec2power(dpwr2); /* POWER_DELAY */
if (mag_flg[A] == 'y') magradpulse(icosel*gzcal*gzlvl2, 0.1*gt1);
else zgradpulse(icosel*gzlvl2, 0.1*gt1); /* 2.0*GRADIENT_DELAY */
rcvron();
statusdelay(C,1.0e-4-rof1);
if (dm3[B] == 'y') {
delay(1/dmf3);
lk_sample();
}
setreceiver(t12);
}
pulsesequence()
{
/* DECLARE VARIABLES */
char autocal[MAXSTR], /* auto-calibration flag */
fsat[MAXSTR],
fscuba[MAXSTR],
f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
fc180[MAXSTR], /* Flag for checking sequence */
ddseq[MAXSTR], /* deuterium decoupling sequence */
spcosed[MAXSTR], /* waveform Co seduce 180 */
spcareb[MAXSTR], /* waveform Ca reburp 180 */
spca180[MAXSTR], /* waveform Ca hard 180 */
sel_flg[MAXSTR],
shp_sl[MAXSTR],
cacb_dec[MAXSTR],
cacbdecseq[MAXSTR],
nietl_flg[MAXSTR];
int phase, phase2, ni, icosel,
t1_counter, /* used for states tppi in t1 */
t2_counter; /* used for states tppi in t2 */
double tau1, /* t1 delay */
tau2, /* t2 delay */
taua, /* ~ 1/4JNH = 2.25 ms */
taub, /* ~ 1/4JNH = 2.25 ms */
tauc, /* ~ 1/4JNCa = ~13 ms */
taud, /* ~ 1/4JCaC' = 3~4.5 ms ms */
bigTN, /* nitrogen T period */
pwc90, /* PW90 for ca nucleus @ d_c90 */
pwca180, /* PW180 for ca nucleus @ d_c180 */
pwca180dec, /* pwca180+pad */
pwcareb, /* pw180 at d_creb ~ 1.6 ms at 600 MHz */
pwcosed, /* PW180 at d_csed ~ 200us at 600 MHz */
tsatpwr, /* low level 1H trans.power for presat */
d_c90, /* power level for 13C pulses(pwc90=sqrt(15)/4delta
delta is the separation between Ca and Co */
d_c180, /* power level for pwca180(sqrt(3)/2delta) */
d_creb, /* power level for pwcareb */
d_csed, /* power level for pwcosed */
sw1, /* sweep width in f1 */
sw2, /* sweep width in f2 */
pw_sl, /* selective pulse on water */
tpwrsl, /* power for pw_sl */
at,
sphase, /* small angle phase shift */
sphase1,
phase_sl,
d_cacbdec,
pwcacbdec,
dres_dec,
pwD, /* PW90 for higher power (pwDlvl) deut 90 */
pwDlvl, /* high power for deut 90 hard pulse */
compC, /* C-13 RF calibration parameters */
pwC,
pwClvl,
pwN, /* PW90 for 15N pulse */
pwNlvl, /* high dec2 pwr for 15N hard pulses */
gstab,
gt1,
gt2,
gt3,
gt4,
gt5,
gt6,
gt7,
gt8,
gt9,
gt10,
gzlvl1,
gzlvl2,
gzlvl3,
gzlvl4,
gzlvl5,
gzlvl6,
gzlvl7,
gzlvl8,
gzlvl9,
gzlvl10;
/* LOAD VARIABLES */
getstr("autocal",autocal);
getstr("fsat",fsat);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("fc180",fc180);
getstr("fscuba",fscuba);
getstr("ddseq",ddseq);
getstr("shp_sl",shp_sl);
getstr("sel_flg",sel_flg);
getstr("cacb_dec",cacb_dec);
getstr("nietl_flg",nietl_flg);
taua = getval("taua");
taub = getval("taub");
tauc = getval("tauc");
taud = getval("taud");
bigTN = getval("bigTN");
pwN = getval("pwN");
tpwr = getval("tpwr");
tsatpwr = getval("tsatpwr");
dpwr = getval("dpwr");
pwNlvl = getval("pwNlvl");
pwD = getval("pwD");
pwDlvl = getval("pwDlvl");
phase = (int) ( getval("phase") + 0.5);
phase2 = (int) ( getval("phase2") + 0.5);
sw1 = getval("sw1");
sw2 = getval("sw2");
ni = getval("ni");
pw_sl = getval("pw_sl");
tpwrsl = getval("tpwrsl");
at = getval("at");
sphase = getval("sphase");
sphase1 = getval("sphase1");
phase_sl = getval("phase_sl");
gstab = getval("gstab");
gt1 = getval("gt1");
if (getval("gt2") > 0) gt2=getval("gt2");
else gt2=gt1*0.1;
gt3 = getval("gt3");
gt4 = getval("gt4");
gt5 = getval("gt5");
gt6 = getval("gt6");
gt7 = getval("gt7");
gt8 = getval("gt8");
gt9 = getval("gt9");
gt10 = getval("gt10");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl3 = getval("gzlvl3");
gzlvl4 = getval("gzlvl4");
gzlvl5 = getval("gzlvl5");
gzlvl6 = getval("gzlvl6");
gzlvl7 = getval("gzlvl7");
gzlvl8 = getval("gzlvl8");
gzlvl9 = getval("gzlvl9");
gzlvl10 = getval("gzlvl10");
if(autocal[0]=='n')
{
getstr("spcosed",spcosed);
getstr("spcareb",spcareb);
getstr("spca180",spca180);
getstr("cacbdecseq",cacbdecseq);
d_c90 = getval("d_c90");
d_c180 = getval("d_c180");
d_creb = getval("d_creb");
d_csed = getval("d_csed");
pwc90 = getval("pwc90");
pwca180 = getval("pwca180");
pwca180dec = getval("pwca180dec");
pwcareb = getval("pwcareb");
pwcosed = getval("pwcosed");
d_cacbdec = getval("d_cacbdec");
pwcacbdec = getval("pwcacbdec");
dres_dec = getval("dres_dec");
}
else
{
strcpy(spcosed,"Phard_118p");
strcpy(spcareb,"Preburp_-15p");
strcpy(spca180,"Phard_-118p");
strcpy(cacbdecseq,"Pcb_dec");
if (FIRST_FID)
{
compC = getval("compC");
pwC = getval("pwC");
pwClvl = getval("pwClvl");
co180 = pbox(spcosed, CO180, CA180ps, dfrq, compC*pwC, pwClvl);
creb = pbox(spcareb, CREB180, CAB180ps, dfrq, compC*pwC, pwClvl);
ca180 = pbox(spca180, CA180, CA180ps, dfrq, compC*pwC, pwClvl);
cbdec = pbox(cacbdecseq, CBDEC,CBDECps, dfrq, compC*pwC, pwClvl);
c90 = pbox("Phard90", C90, CA180ps, dfrq, compC*pwC, pwClvl);
}
d_c90 = c90.pwr;
d_c180 = ca180.pwr;
d_creb = creb.pwr;
d_csed = co180.pwr;
pwc90 = c90.pw;
pwca180 = ca180.pw;
pwca180dec = ca180.pw;
pwcareb = creb.pw;
pwcosed = co180.pw;
d_cacbdec = cbdec.pwr;
pwcacbdec = 1.0/cbdec.dmf;
dres_dec = cbdec.dres;
}
/* LOAD PHASE TABLE */
settable(t1,2,phi1);
settable(t2,4,phi2);
settable(t3,8,phi3);
settable(t4,2,phi4);
settable(t5,1,phi5);
settable(t6,8,rec);
/* CHECK VALIDITY OF PARAMETER RANGES */
if(ix==1)
printf("Uses shared AT in the N dimension. Choose ni2 as desired\n");
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y' ))
{
printf("incorrect dec1 decoupler flags! ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y'))
{
printf("incorrect dec2 decoupler flags! Should be 'nnn' ");
psg_abort(1);
}
if( tsatpwr > 6 )
{
printf("TSATPWR too large !!! ");
psg_abort(1);
}
if( dpwr > -16 )
{
printf("DPWR too large! ");
psg_abort(1);
}
if( dpwr2 > -16 )
{
printf("DPWR2 too large! ");
psg_abort(1);
}
if( pw > 200.0e-6 )
{
printf("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwN > 200.0e-6 )
{
printf("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
if( gt1 > 3e-3 || gt2 > 3e-3 || gt3 > 3e-3
|| gt4 > 3e-3 || gt5 > 3e-3 || gt6 > 3e-3
|| gt7 > 3e-3 || gt8 > 3e-3 || gt9 > 3e-3 || gt10 > 3e-3)
{
printf("gti values must be < 3e-3\n");
psg_abort(1);
}
if(tpwrsl > 30) {
printf("tpwrsl must be less than 25\n");
psg_abort(1);
}
if( pwDlvl > 59) {
printf("pwDlvl too high\n");
psg_abort(1);
}
if( dpwr3 > 50) {
printf("dpwr3 too high\n");
psg_abort(1);
}
if( pw_sl > 10e-3) {
printf("too long pw_sl\n");
psg_abort(1);
}
if(d_cacbdec > 40) {
printf("d_cacbdec is too high; < 41\n");
psg_abort(1);
}
if(nietl_flg[A] == 'y' && sel_flg[A] == 'y') {
printf("nietl_flg and sel_flg cannot both be y\n");
psg_abort(1);
}
if (fc180[A] =='y' && ni > 1.0) {
text_error("must set fc180='n' to allow C' evolution (ni>1)\n");
psg_abort(1);
}
/* Phase incrementation for hypercomplex 2D data */
if (phase == 2) tsadd(t2,1,4);
if (phase2 == 2) {
tsadd(t5,2,4);
icosel = 1;
}
else icosel = -1;
if (nietl_flg[A] == 'y') icosel = -1*icosel;
/* Set up f1180 tau2 = t1 */
tau1 = d2;
if(f1180[A] == 'y') {
tau1 += ( 1.0 / (2.0*sw1)
- 4.0/PI*pwc90 - POWER_DELAY - 4.0e-6 - WFG_START_DELAY
- pwca180dec - WFG_STOP_DELAY - 2.0*pwN - POWER_DELAY
- 4.0e-6);
}
if(f1180[A] == 'n')
tau1 = ( tau1
- 4.0/PI*pwc90 - POWER_DELAY - 4.0e-6 - WFG_START_DELAY
- pwca180dec - WFG_STOP_DELAY - 2.0*pwN - POWER_DELAY
- 4.0e-6);
if(tau1 < 0.2e-6) tau1 = 0.2e-6;
tau1 = tau1/2.0;
/* Set up f2180 tau2 = t2 */
tau2 = d3;
if(f2180[A] == 'y') {
tau2 += ( 1.0 / (2.0*sw2) );
if(tau2 < 0.2e-6) tau2 = 0.2e-6;
}
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2 ;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2) {
tsadd(t2,2,4);
tsadd(t6,2,4);
}
if( ix == 1) d3_init = d3 ;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2) {
tsadd(t3,2,4);
tsadd(t6,2,4);
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tsatpwr); /* Set transmitter power for 1H presaturation */
decpower(d_c180); /* Set Dec1 power to high power */
dec2power(pwNlvl); /* Set Dec2 power for 15N hard pulses */
decoffset(dof);
/* Presaturation Period */
if (fsat[0] == 'y')
{
delay(2.0e-5);
rgpulse(d1,zero,2.0e-6,2.0e-6);
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
delay(2.0e-5);
if(fscuba[0] == 'y')
{
delay(2.2e-2);
rgpulse(pw,zero,2.0e-6,0.0);
rgpulse(2*pw,one,2.0e-6,0.0);
rgpulse(pw,zero,2.0e-6,0.0);
delay(2.2e-2);
}
}
else
{
delay(d1);
}
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
txphase(zero);
dec2phase(zero);
delay(1.0e-5);
/* Begin Pulses */
status(B);
rcvroff();
lk_hold();
delay(20.0e-6);
initval(1.0,v2);
obsstepsize(phase_sl);
xmtrphase(v2);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shp_sl,pw_sl,one,4.0e-6,0.0);
xmtrphase(zero);
obspower(tpwr);
txphase(zero);
delay(4.0e-6);
/* shaped pulse */
rgpulse(pw,zero,0.0,0.0); /* 90 deg 1H pulse */
delay(0.2e-6);
zgradpulse(gzlvl5,gt5);
delay(2.0e-6);
delay(taua - gt5 - 2.2e-6); /* taua <= 1/4JNH */
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
txphase(three);
dec2phase(zero);
decphase(zero);
delay(taua - gt5 - 200.2e-6 - 2.0e-6);
delay(0.2e-6);
zgradpulse(gzlvl5,gt5);
delay(200.0e-6);
if (sel_flg[A] == 'n')
{
rgpulse(pw,three,2.0e-6,0.0);
decpower(d_c180);
delay(0.2e-6);
zgradpulse(gzlvl3,gt3);
delay(200.0e-6);
dec2rgpulse(pwN,zero,0.0,0.0);
delay(tauc);
dec2rgpulse(2*pwN,zero,0.0,0.0);
decrgpulse(pwca180,zero,0.0,0.0);
dec2phase(one);
delay(tauc - pwca180);
dec2rgpulse(pwN,one,0.0,0.0);
}
else
{
rgpulse(pw,one,2.0e-6,0.0);
decpower(d_c180);
initval(1.0,v5);
dec2stepsize(45.0);
dcplr2phase(v5);
delay(0.2e-6);
zgradpulse(gzlvl3,gt3);
delay(200.0e-6);
dec2rgpulse(pwN,zero,0.0,0.0);
dcplr2phase(zero);
delay(1.34e-3 - SAPS_DELAY - 2.0*pw);
rgpulse(pw,one,0.0,0.0);
rgpulse(2.0*pw,zero,0.0,0.0);
rgpulse(pw,one,0.0,0.0);
delay(tauc - 1.34e-3 - 2.0*pw);
dec2rgpulse(2*pwN,zero,0.0,0.0);
decrgpulse(pwca180,zero,0.0,0.0);
dec2phase(one);
delay(tauc - pwca180);
dec2rgpulse(pwN,one,0.0,0.0);
}
/* END sel_flg */
decphase(t1);
decpower(d_c90);
delay(0.2e-6);
zgradpulse(gzlvl8,gt8);
delay(200.0e-6);
/* Cay to CaxC'z */
dec2phase(zero);
txphase(zero);
/* Turn on D decoupling using the third decoupler */
dec3phase(one);
dec3power(pwDlvl);
dec3rgpulse(pwD,one,4.0e-6,0.0);
dec3phase(zero);
dec3power(dpwr3);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
/* Turn on D decoupling */
if (cacb_dec[A] == 'n')
{
decrgpulse(pwc90,t1,2.0e-6,0.0);
delay(taud -POWER_DELAY -4.0e-6 -WFG_START_DELAY);
initval(1.0,v3);
decstepsize(sphase);
dcplrphase(v3);
decpower(d_creb);
decshaped_pulse(spcareb,pwcareb,zero,4.0e-6,0.0);
dcplrphase(zero);
decpower(d_csed);
decshaped_pulse(spcosed,pwcosed,zero,4.0e-6,0.0);
delay(taud - WFG_STOP_DELAY
- POWER_DELAY - 4.0e-6 - WFG_START_DELAY - pwcosed - WFG_STOP_DELAY
- POWER_DELAY - 2.0e-6);
decpower(d_c90);
decrgpulse(pwc90,one,2.0e-6,0.0);
}
else
{
decrgpulse(pwc90,t1,2.0e-6,0.0);
/* CaCb dec on */
decpower(d_cacbdec);
decprgon(cacbdecseq,pwcacbdec,dres_dec);
decon();
/* CaCb dec on */
delay(taud - POWER_DELAY - PRG_START_DELAY
- PRG_STOP_DELAY
- POWER_DELAY - 4.0e-6 - WFG_START_DELAY);
/* CaCb dec off */
decoff();
decprgoff();
/* CaCb dec off */
initval(1.0,v3);
decstepsize(sphase);
dcplrphase(v3);
decpower(d_creb);
decshaped_pulse(spcareb,pwcareb,zero,4.0e-6,0.0);
dcplrphase(zero);
decpower(d_csed);
decshaped_pulse(spcosed,pwcosed,zero,4.0e-6,0.0);
/* CaCb dec on */
decpower(d_cacbdec);
decprgon(cacbdecseq,pwcacbdec,dres_dec);
decon();
/* CaCb dec on */
delay(taud - WFG_STOP_DELAY
- POWER_DELAY - 4.0e-6 - WFG_START_DELAY - pwcosed - WFG_STOP_DELAY
- POWER_DELAY - PRG_START_DELAY
- PRG_STOP_DELAY
- POWER_DELAY - 2.0e-6);
/* CaCb dec off */
decoff();
decprgoff();
/* CaCb dec off */
decpower(d_c90);
decrgpulse(pwc90,one,2.0e-6,0.0);
}
/* END cacb_dec */
/* Turn off D decoupling */
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3blank();
dec3phase(three);
dec3power(pwDlvl);
dec3rgpulse(pwD,three,4.0e-6,0.0);
/* Turn off D decoupling */
decoffset(dof+(174-56)*dfrq); /* change Dec1 carrier to Co */
delay(2.0e-7);
zgradpulse(gzlvl4,gt4);
delay(100.0e-6);
/* t1 period for C' chemical shift evolution; Ca 180 and N 180 are used
to decouple */
decrgpulse(pwc90,t2,2.0e-6,0.0);
if (fc180[A]=='n')
{
decpower(d_c180);
delay(tau1);
decshaped_pulse(spca180,pwca180dec,zero,4.0e-6,0.0);
dec2rgpulse(2*pwN,zero,0.0,0.0);
delay(tau1);
decpower(d_c90);
}
else
decrgpulse(2*pwc90,zero,0.0,0.0);
decrgpulse(pwc90,zero,4.0e-6,0.0);
decoffset(dof); /* set carrier to Ca */
delay(2.0e-7);
zgradpulse(gzlvl9,gt9);
delay(100.0e-6);
/* Refocusing CayC'z to Cax */
/* Turn on D decoupling using the third decoupler */
dec3phase(one);
dec3power(pwDlvl);
dec3rgpulse(pwD,one,4.0e-6,0.0);
dec3phase(zero);
dec3power(dpwr3);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
/* Turn on D decoupling */
if (cacb_dec[A] == 'n')
{
decrgpulse(pwc90,zero,0.0e-6,0.0);
delay(taud - POWER_DELAY
- 4.0e-6 - WFG_START_DELAY - pwcosed - WFG_STOP_DELAY
- POWER_DELAY - 4.0e-6 - WFG_START_DELAY);
decpower(d_csed);
decshaped_pulse(spcosed,pwcosed,zero,4.0e-6,0.0);
decpower(d_creb);
initval(1.0,v4);
decstepsize(sphase1);
dcplrphase(v4);
decshaped_pulse(spcareb,pwcareb,zero,4.0e-6,0.0);
dcplrphase(zero);
delay(taud - WFG_STOP_DELAY
- POWER_DELAY
- 4.0e-6);
decpower(d_c90);
decrgpulse(pwc90,one,4.0e-6,0.0);
}
else
{
decrgpulse(pwc90,zero,0.0e-6,0.0);
/* CaCb dec on */
decpower(d_cacbdec);
decprgon(cacbdecseq,pwcacbdec,dres_dec);
decon();
/* CaCb dec on */
delay(taud
- POWER_DELAY - PRG_START_DELAY
- PRG_STOP_DELAY
- POWER_DELAY
- 4.0e-6 - WFG_START_DELAY - pwcosed - WFG_STOP_DELAY
- POWER_DELAY - 4.0e-6 - WFG_START_DELAY);
/* CaCb dec off */
decoff();
decprgoff();
/* CaCb dec off */
decpower(d_csed);
decshaped_pulse(spcosed,pwcosed,zero,4.0e-6,0.0);
decpower(d_creb);
initval(1.0,v4);
decstepsize(sphase1);
dcplrphase(v4);
decshaped_pulse(spcareb,pwcareb,zero,4.0e-6,0.0);
dcplrphase(zero);
/* CaCb dec on */
decpower(d_cacbdec);
decprgon(cacbdecseq,pwcacbdec,dres_dec);
decon();
/* CaCb dec on */
delay(taud - WFG_STOP_DELAY
- POWER_DELAY - PRG_START_DELAY
- PRG_STOP_DELAY
- POWER_DELAY
- 4.0e-6);
/* CaCb dec off */
decoff();
decprgoff();
/* CaCb dec off */
decpower(d_c90);
decrgpulse(pwc90,one,4.0e-6,0.0);
}
/* END cacb_dec */
/* Turn off D decoupling */
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3blank();
dec3phase(three);
dec3power(pwDlvl);
dec3rgpulse(pwD,three,4.0e-6,0.0);
/* Turn off D decoupling */
decpower(d_c180);
txphase(zero);
delay(2.0e-7);
zgradpulse(gzlvl10,gt10);
delay(100.0e-6);
/* Constant t2 period */
if (bigTN - tau2 >= 0.2e-6)
{
dec2rgpulse(pwN,t3,2.0e-6,0.0);
dec2phase(t4);
delay(bigTN - tau2 + pwca180);
dec2rgpulse(2*pwN,t4,0.0,0.0);
decrgpulse(pwca180,zero,0.0,0.0);
dec2phase(t5);
decpower(d_csed);
delay(bigTN - gt1 - 502.0e-6 - 2.0*GRADIENT_DELAY - POWER_DELAY
- WFG_START_DELAY - pwcosed - WFG_STOP_DELAY);
delay(2.0e-6);
zgradpulse(gzlvl1,gt1);
delay(500.0e-6);
decshaped_pulse(spcosed,pwcosed,zero,0.0,0.0);
delay(tau2);
sim3pulse(pw,0.0e-6,pwN,zero,zero,t5,0.0,0.0);
}
else
{
dec2rgpulse(pwN,t3,2.0e-6,0.0);
dec2rgpulse(2.0*pwN,t4,2.0e-6,2.0e-6);
dec2phase(t5);
delay(tau2 - bigTN);
decrgpulse(pwca180,zero,0.0,0.0);
decpower(d_csed);
delay(bigTN - pwca180 - POWER_DELAY
- gt1 - 502.0e-6 - 2.0*GRADIENT_DELAY
- WFG_START_DELAY - pwcosed - WFG_STOP_DELAY);
delay(2.0e-6);
zgradpulse(gzlvl1,gt1);
delay(500.0e-6);
decshaped_pulse(spcosed,pwcosed,zero,0.0,0.0);
delay(tau2);
sim3pulse(pw,0.0e-6,pwN,zero,zero,t5,0.0,0.0);
}
if (nietl_flg[A] == 'n')
{
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(2.0e-6);
dec2phase(zero);
delay(taub - gt6 - 2.2e-6);
sim3pulse(2*pw,0.0e-6,2*pwN,zero,zero,zero,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(200.0e-6);
delay(taub - gt6 - 200.2e-6);
txphase(one);
dec2phase(one);
sim3pulse(pw,0.0e-6,pwN,one,zero,one,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
txphase(zero);
dec2phase(zero);
delay(taub - gt7 - 2.2e-6);
sim3pulse(2*pw,0.0e-6,2*pwN,zero,zero,zero,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(200.0e-6);
delay(taub - gt7 - 200.2e-6);
sim3pulse(pw,0.0e-6,pwN,zero,zero,zero,0.0,0.0);
}
else
{
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shp_sl,pw_sl,zero,4.0e-6,0.0);
obspower(tpwr);
txphase(zero);
delay(4.0e-6);
/* shaped pulse */
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(2.0e-6);
dec2phase(zero);
delay(taub - POWER_DELAY - 4.0e-6 - WFG_START_DELAY - pw_sl
- WFG_STOP_DELAY - POWER_DELAY - 4.0e-6
- gt6 - 2.2e-6);
sim3pulse(2*pw,0.0e-6,2*pwN,zero,zero,zero,0.0,0.0);
txphase(one);
dec2phase(zero);
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(200.0e-6);
delay(taub - gt6 - 200.2e-6);
sim3pulse(pw,0.0e-6,pwN,one,zero,zero,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
txphase(zero);
dec2phase(zero);
delay(taub - gt7 - 2.2e-6);
sim3pulse(2*pw,0.0e-6,2*pwN,zero,zero,zero,0.0,0.0);
txphase(one);
dec2phase(one);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(200.0e-6);
delay(taub - gt7 - 200.2e-6);
sim3pulse(pw,0.0e-6,pwN,one,zero,one,0.0,0.0);
txphase(zero);
}
delay(gt2 +gstab -0.5*(pwN -pw) -2.0*pw/PI);
rgpulse(2*pw,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(icosel*gzlvl2, gt2);
decpower(dpwr);
dec2power(dpwr2);
delay(gstab -2.0e-6 -2.0*GRADIENT_DELAY -2.0*POWER_DELAY);
lk_sample();
status(C);
setreceiver(t6);
}
pulsesequence()
{
/* DECLARE VARIABLES */
char
SE[MAXSTR], /* coherence gradients & sensitivity enhance */
CT[MAXSTR], /* constant time in t1 */
CCdseq[MAXSTR],
CChomodec[MAXSTR], /* Setup for C-imino - C-H6 */
C13refoc[MAXSTR], /* C13 pulse in middle of t1*/
f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR]; /* Flag to start t1 @ halfdwell */
int icosel,
ni2 = getval("ni2"),
t1_counter,
t2_counter;
double tau1, /* t1 delay */
tau2, /* t2 delay */
lambda = 0.94/(4.0*getval("JCH")), /* 1/4J C-H INEPT delay */
CTdelay = getval("CTdelay"), /* total constant time evolution */
CCdpwr = getval("CCdpwr"), /* power level for CC decoupling */
CCdres = getval("CCdres"), /* dres for CC decoupling */
CCdmf = getval("CCdmf"), /* dmf for CC decoupling */
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
pwZa, /* the largest of 2.0*pw and 2.0
*pwN */
tpwr = getval("tpwr"), /* power for H1 pulses */
pw = getval("pw"), /* H1 90 degree pulse length at tpwr */
compH = getval("compH"), /* adjustment for H1 amplifier compression */
ncyc_cc = getval("ncyc_cc"), /* number of DIPSI3 cycles for CC spinlock */
tof_75, /* tof shifted to 7.5 ppm for H4-N4 transfer */
tof_12, /* tof shifted to 12 ppm for H3-N3 transfer */
dof_80, /* dof shifted to 169 ppm for N3-C4 transfer */
dof_92p5, /* dof shifted to 92.5ppm */
/* p_d is used to calculate the isotropic mixing */
p_d, /* 50 degree pulse for DIPSI-3 at rfdC */
p_d2, /* 50 degree pulse for DIPSI-3 at rfd */
rfd, /* fine C13 power for 10 kHz rf for 500MHz magnet */
sw1 = getval("sw1"),
sw2 = getval("sw2"),
gstab = getval("gstab"),
pwHs = getval("pwHs"), /* H1 90 degree pulse length at tpwrs */
tpwrs, /* power for the pwHs ("rna_H2Osinc") pulse */
pwHs2 = getval("pwHs2"), /* H1 90 degree pulse length at tpwrs2 */
tpwrs2, /* power for the pwHs2 square pulse */
gt1 = getval("gt1"), /* coherence pathway gradients */
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gzlvl0 = getval("gzlvl0"),
gzlvl3 = getval("gzlvl3"),
gt3 = getval("gt3"),
gzlvl4 = getval("gzlvl4"),
gt4 = getval("gt4"),
gzlvl5 = getval("gzlvl5"),
gzlvl6 = getval("gzlvl6"),
gt5 = getval("gt5"),
gzlvlr = getval("gzlvlr");
getstr("SE",SE);
getstr("CT",CT);
getstr("CChomodec",CChomodec);
getstr("CCdseq",CCdseq);
getstr("C13refoc",C13refoc);
getstr("f1180",f1180);
getstr("f2180",f2180);
/* LOAD PHASE TABLE */
settable(t1,2,phi1);
settable(t3,8,phi3);
settable(t4,16,phi4);
settable(t9,1,phi9);
settable(t10,1,phi10);
settable(t5,4,phi5);
settable(t11,8,rec1);
/* INITIALIZE VARIABLES */
/* different offset values tof=H2O, dof=110ppm, dof2=200ppm */
tof_75 = tof + 2.5*sfrq; /* tof shifted to nH2 */
tof_12 = tof + 8.0*sfrq; /* tof shifted to nH */
dof_92p5 = dof - 17.5*dfrq; /* dof shifted to C1' */
dof_80 = dof - 30*dfrq; /* dof shifted to C6 */
/* 1.9 kHz DIPSI-3 at 500MHz scaled to this sfrq*/
p_d = (5.0)/(9.0*4.0*1900.0*(sfrq/500.0));
/* 7 kHz DIPSI-3 at 500MHz scaled to this sfrq*/
p_d2 = (5.0)/(9.0*4.0*7000.0*(sfrq/500.0));
ncyc_cc = (int) (ncyc_cc + 0.5);
if (ncyc_cc > 0 )
{
printf("CC-mixing time is %f ms.\n",(ncyc_cc*51.8*4*p_d2));
}
if( ncyc_cc > 12 )
{
text_error("check C->C dipsi-3 time !! ");
psg_abort(1);
}
initval(ncyc_cc,v2);
/* fine C13 power for dipsi-3 isotropic mixing */
rfd = (compC*4095.0*pwC*5.0)/(p_d2*9.0);
rfd = (int) (rfd + 0.5);
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /* needs 1.69 times more */
tpwrs = (int) (tpwrs); /* power than a square pulse */
/* selective H20 square pulse */
tpwrs2 = tpwr - 20.0*log10(pwHs2/(compH*pw));
tpwrs2 = (int) (tpwrs2);
if (2.0*pw > 2.0*pwN) pwZa = 2.0*pw;
else pwZa = 2.0*pwN;
if ((CT[A]=='y') && (ni2/(4.0*sw2) > CTdelay))
{ text_error( " ni2 is too big. Make ni2 equal to %d or less.\n",
((int)(CTdelay*sw2*4.0)) ); psg_abort(1); }
/* PHASES AND INCREMENTED TIMES */
/* Phase incrementation for hypercomplex 2D data, States-Haberkorn element */
if (phase1 == 2)
tsadd(t5,1,4);
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{ tsadd(t5,2,4); tsadd(t11,2,4); }
/* Set up f1180 */
tau1 = d2;
if((f1180[A] == 'y') && (ni > 1.0))
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
tau1 = tau1/2.0;
icosel=1;
if (SE[A]=='y')
{
if (phase2 == 2)
{
tsadd(t10,2,4);
icosel = -1;
}
}
/* Set up f2180 */
tau2 = d3;
if((f2180[A] == 'y') && (ni2 > 1.0))
{
tau2 += ( 1.0 / (2.0*sw2) );
if(tau2 < 0.2e-6) tau2 = 0.0;
}
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d3_init = d3;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2)
{
tsadd(t3,2,4);
tsadd(t11,2,4);
}
/* CHECK VALIDITY OF PARAMETER RANGE */
if( sfrq > 610 )
{ printf("Power Levels at 750/800 MHz may be too high for probe");
psg_abort(1); }
if( dpwrf < 4095 )
{ printf("reset dpwrf=4095 and recalibrate C13 90 degree pulse");
psg_abort(1); }
if( dpwrf2 < 4095 )
{ printf("reset dpwrf2=4095 and recalibrate N15 90 degree pulse");
psg_abort(1); }
if((dm[A] == 'y' || dm[B] == 'y'))
{
printf("incorrect dec1 decoupler flag! Should be 'nny' or 'nnn' ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y'))
{
printf("incorrect dec2 decoupler flags! Should be 'nnn' or 'nny' ");
psg_abort(1);
}
if( ((dm[C] == 'y') && (dm2[C] == 'y') && (at > 0.18)) )
{
text_error("check at time! Don't fry probe !! ");
psg_abort(1);
}
if( dpwr > 50 )
{
printf("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if( dpwr2 > 50 )
{
printf("don't fry the probe, DPWR2 too large! ");
psg_abort(1);
}
if( pw > 20.0e-6 )
{
printf("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwC > 40.0e-6 )
{
printf("dont fry the probe, pwC too high ! ");
psg_abort(1);
}
if( pwN > 100.0e-6 )
{
printf("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
if (gzlvlr > 500 || gzlvlr < -500)
{
text_error(" RDt1-gzlvlr must be -500 to 500 (0.5G/cm) \n");
psg_abort(1);
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
obsstepsize(0.5);
decpower(pwClvl);
decstepsize(0.5);
obsoffset(tof);
dec2power(pwNlvl);
dec2stepsize(0.5);
decoffset(dof_80); /* Preset the carbon frequency for the C1' carbons */
txphase(zero);
decphase(zero);
dec2phase(zero);
delay(d1);
rcvroff();
dec2rgpulse(pwN, zero, 0.0, 0.0); /*destroy N15 and C13 magnetization*/
decrgpulse(pwC, zero, 0.0, 0.0);
zgradpulse(gzlvl0, 0.5e-3);
delay(1.0e-4);
dec2rgpulse(pwN, one, 0.0, 0.0);
decrgpulse(pwC, one, 0.0, 0.0);
zgradpulse(0.7*gzlvl0, 0.5e-3);
delay(5.0e-4);
delay(lambda);
simpulse(2*pw, 2*pwC, zero, zero, 0.0, 0.0);
dec2phase(t5);
delay(lambda - SAPS_DELAY);
simpulse(pw, pwC, zero, t5, 0.0, 0.0); /* 2x, -2x*/
dec2phase(zero);
txphase(one);
zgradpulse(gzlvl5,gt5);
delay(lambda - SAPS_DELAY - gt5);
simpulse(2*pw, 2*pwC, one, zero, 0.0, 0.0);
zgradpulse(gzlvl5,gt5);
delay(lambda - 2*SAPS_DELAY - gt5 - 2*POWER_DELAY);
decpwrf(4095.0);
txphase(zero);
decphase(zero);
if (C13refoc[A]=='y')
{
if (tau1 > (2.0*GRADIENT_DELAY + pwN + 0.64*pw + 5.0*SAPS_DELAY))
{
zgradpulse(gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw) - SAPS_DELAY);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(-1.0*gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
}
else if (tau1 > (0.64*pw + 0.5*SAPS_DELAY))
delay(2.0*tau1 - 2.0*0.64*pw - SAPS_DELAY );
}
else
{
if (tau1 > (2.0*GRADIENT_DELAY + pwN + 0.64*pw + 5.0*SAPS_DELAY))
{
zgradpulse(gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw) - SAPS_DELAY);
dec2rgpulse(2.0*pwN, zero, 0.0, 0.0);
zgradpulse(-1.0*gzlvlr, 0.8*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
delay(0.2*(tau1 - 2.0*GRADIENT_DELAY - pwN - 0.64*pw));
}
else if (tau1 > (0.64*pw + 0.5*SAPS_DELAY))
delay(2.0*tau1 - 2.0*0.64*pw - SAPS_DELAY );
}
decrgpulse(pwC,three,0.0,0.0); /* flip transferred 13C-magn. to z */
decrgpulse(pwC,one,0.0,0.0); /* flip transferred 13C-magn. to z */
decphase(zero);
decpwrf(rfd);
starthardloop(v2);
decrgpulse(6.4*p_d2,zero,0.0,0.0);
decrgpulse(8.2*p_d2,two,0.0,0.0);
decrgpulse(5.8*p_d2,zero,0.0,0.0);
decrgpulse(5.7*p_d2,two,0.0,0.0);
decrgpulse(0.6*p_d2,zero,0.0,0.0);
decrgpulse(4.9*p_d2,two,0.0,0.0);
decrgpulse(7.5*p_d2,zero,0.0,0.0);
decrgpulse(5.3*p_d2,two,0.0,0.0);
decrgpulse(7.4*p_d2,zero,0.0,0.0);
decrgpulse(6.4*p_d2,two,0.0,0.0);
decrgpulse(8.2*p_d2,zero,0.0,0.0);
decrgpulse(5.8*p_d2,two,0.0,0.0);
decrgpulse(5.7*p_d2,zero,0.0,0.0);
decrgpulse(0.6*p_d2,two,0.0,0.0);
decrgpulse(4.9*p_d2,zero,0.0,0.0);
decrgpulse(7.5*p_d2,two,0.0,0.0);
decrgpulse(5.3*p_d2,zero,0.0,0.0);
decrgpulse(7.4*p_d2,two,0.0,0.0);
decrgpulse(6.4*p_d2,two,0.0,0.0);
decrgpulse(8.2*p_d2,zero,0.0,0.0);
decrgpulse(5.8*p_d2,two,0.0,0.0);
decrgpulse(5.7*p_d2,zero,0.0,0.0);
decrgpulse(0.6*p_d2,two,0.0,0.0);
decrgpulse(4.9*p_d2,zero,0.0,0.0);
decrgpulse(7.5*p_d2,two,0.0,0.0);
decrgpulse(5.3*p_d2,zero,0.0,0.0);
decrgpulse(7.4*p_d2,two,0.0,0.0);
decrgpulse(6.4*p_d2,zero,0.0,0.0);
decrgpulse(8.2*p_d2,two,0.0,0.0);
decrgpulse(5.8*p_d2,zero,0.0,0.0);
decrgpulse(5.7*p_d2,two,0.0,0.0);
decrgpulse(0.6*p_d2,zero,0.0,0.0);
decrgpulse(4.9*p_d2,two,0.0,0.0);
decrgpulse(7.5*p_d2,zero,0.0,0.0);
decrgpulse(5.3*p_d2,two,0.0,0.0);
decrgpulse(7.4*p_d2,zero,0.0,0.0);
endhardloop();
decphase(t3);
decpwrf(4095.0);
decrgpulse(pwC,three,0.0,0.0); /* flip transferred 13C-magnetization to z */
decoffset(dof_92p5); /* Preset the carbon frequency for the C1' carbon */
decrgpulse(pwC,t3,0.0,0.0); /* 4x,-4x flip transferred 13C-magnetization to x */
if (SE[A]=='y')
{
/***************** CONSTANT TIME EVOLUTION *****************/
if (CT[A]=='y') {
/***************/
initval(90.0, v9);
decstepsize(1.0);
dcplrphase(v9);
decphase(t9);
delay(CTdelay/2.0 - tau2);
decrgpulse(2.0*pwC, t9, 0.0, 0.0);
dcplrphase(zero);
decphase(t10);
if (tau2 < gt1 + gstab)
{delay(CTdelay/2.0 - pwZa - gt1 - gstab);
zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
delay(gstab - 2.0*GRADIENT_DELAY);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
delay(tau2);}
else {delay(CTdelay/2.0 - pwZa);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
delay(tau2 - gt1 - gstab);
zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
delay(gstab - 2.0*GRADIENT_DELAY);}
/***************/
}
/********************************************************************/
/***************** NORMAL EVOLUTION *****************/
else {
/***************/
if (CChomodec[A]=='y')
{
decpower(CCdpwr); decphase(zero);
decprgon(CCdseq,1.0/CCdmf,CCdres);
decon(); /* CC decoupling on */
}
decphase(zero);
delay(tau2);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
decphase(t9);
delay(gt1 + gstab - pwZa);
delay(tau2);
if (CChomodec[A]=='y')
{
decoff(); decprgoff(); /* CC decoupling off */
decpower(pwClvl);
}
decrgpulse(2.0*pwC, t9, 0.0, 0.0);
zgradpulse(icosel*gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
decphase(t10);
delay(gstab - 2.0*GRADIENT_DELAY);
/***************/
}
/********************************************************************/
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
decrgpulse(pwC, zero, 0.0, 0.0);
decphase(zero);
zgradpulse(gzlvl5, gt5);
delay(lambda - 0.5*pwC - gt5);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
txphase(one);
decphase(t10);
delay(lambda - 0.5*pwC - gt5);
simpulse(pw, pwC, one, t10, 0.0, 0.0);
txphase(zero);
decphase(zero);
zgradpulse(gzlvl6, gt5);
delay(lambda - 0.5*pwC - gt5);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
txphase(two);
zgradpulse(gzlvl6, gt5);
delay(lambda - 0.5*pwC - gt5);
simpulse(pw, pwC, two, zero, 0.0, 0.0);
txphase(zero);
delay(lambda - 0.5*pwC);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
dcplrphase(zero); /* SAPS_DELAY */
zgradpulse(gzlvl2, gt1/4.0); /* 2.0*GRADIENT_DELAY */
delay(lambda - gt1/4.0 - 0.5*pwC - 2.0*GRADIENT_DELAY - 2*POWER_DELAY - SAPS_DELAY);
}
else
{
decphase(zero);
zgradpulse(gzlvl5,gt5);
delay(lambda - gt5);
simpulse(2*pw,2*pwC,zero,zero,0.0,0.0);
zgradpulse(gzlvl5,gt5);
delay(lambda - gt5);
decrgpulse(pwC,zero,0.0,0.0);
zgradpulse(gzlvl3,gt3);
delay(gstab);
obspower(tpwrs);
shaped_pulse("rna_H2Osinc", pwHs, zero, 5.0e-4, 0.0);
obspower(tpwr);
rgpulse(pw, zero, 2*rof1, 0.0);
txphase(two);
obspower(tpwrs2);
zgradpulse(gzlvl4,gt4);
delay(gstab - 2*SAPS_DELAY - 2*POWER_DELAY - GRADIENT_DELAY);
rgpulse((lambda-gstab-gt4-pwC), two, 0.0, 0.0);
simpulse(pwC,pwC,two,three,0.0,0.0);
simpulse(2*pwC,2*pwC,two,zero,0.0,0.0);
simpulse(pwC,pwC,two,three,0.0,0.0);
rgpulse((pwHs2-2*pwC-(lambda-gstab-gt4-pwC)), two, 0.0, 0.0);
txphase(zero);
obspower(tpwr);
rgpulse(2*pw, zero, 0.0, 0.0);
obspower(tpwrs2);
rgpulse(pwHs2, two, 4.0e-6, 0.0);
decphase(t4);
zgradpulse(gzlvl4,gt4);
delay(gstab-2*pwC-2*SAPS_DELAY - POWER_DELAY - GRADIENT_DELAY);
decrgpulse(pwC,t4,0.0,0.0);
decrgpulse(pwC,zero,0.0,0.0);
}
dec2power(dpwr2); /* 2*POWER_DELAY */
decpower(dpwr);
status(C);
setreceiver(t11);
}
pulsesequence()
{
/* DECLARE AND LOAD VARIABLES */
char f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
mag_flg[MAXSTR], /* magic-angle coherence transfer gradients */
TROSY[MAXSTR]; /* To check for TROSY flag */
int icosel, /* used to get n and p type */
t1_counter, /* used for states tppi in t1 */
t2_counter, /* used for states tppi in t2 */
ni2 = getval("ni2");
double p_d,
rfd,
ncyc,
COmix = getval("COmix"),
p_trim,
rftrim,
tau1, /* t1 delay */
tau2, /* t2 delay */
timeTN = getval("timeTN"), /* constant time for 15N evolution */
kappa = 5.4e-3,
lambda = 2.4e-3,
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rf0, /* maximum fine power when using pwC pulses */
bw, ofs, ppm, /* bandwidth, offset, ppm - temporary Pbox parameters */
/* the following pulse lengths for SLP pulses are automatically calculated */
/* by the macro "biocal". SLP pulse shapes, "offC3" etc are called */
/* directly from your shapelib. */
pwC3 = getval("pwC3"), /*180 degree pulse at Ca(56ppm) null at CO(174ppm) */
pwC3a, /* pwC3a=pwC3, but not set to zero when pwC3=0 */
phshift3, /* phase shift induced on CO by pwC3 ("offC3") pulse */
pwZ, /* the largest of pwC3 and 2.0*pwN */
pwZ1, /* the largest of pwC3a and 2.0*pwN for 1D experiments */
pwC6, /* 90 degree selective sinc pulse on CO(174ppm) */
pwC8, /* 180 degree selective sinc pulse on CO(174ppm) */
rf3, /* fine power for the pwC3 ("offC3") pulse */
rf6, /* fine power for the pwC6 ("offC6") pulse */
rf8, /* fine power for the pwC8 ("offC8") pulse */
compH = getval("compH"), /* adjustment for C13 amplifier compression */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
pwHs = getval("pwHs"), /* H1 90 degree pulse length at tpwrs */
tpwrsf = getval("tpwrsf"), /* fine power adjustment for flipback */
tpwrs, /* power for the pwHs ("H2Osinc") pulse */
pwHd, /* H1 90 degree pulse length at tpwrd */
tpwrd, /* rf for WALTZ decoupling */
waltzB1 = getval("waltzB1"), /* waltz16 field strength (in Hz) */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
sw1 = getval("sw1"),
sw2 = getval("sw2"),
gt1 = getval("gt1"), /* coherence pathway gradients */
gzcal = getval("gzcal"), /* g/cm to DAC conversion factor */
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gt0 = getval("gt0"), /* other gradients */
gt3 = getval("gt3"),
gt4 = getval("gt4"),
gt5 = getval("gt5"),
gstab = getval("gstab"),
gzlvl0 = getval("gzlvl0"),
gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5"),
gzlvl6 = getval("gzlvl6");
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("mag_flg",mag_flg);
getstr("TROSY",TROSY);
/* LOAD PHASE TABLE */
settable(t3,2,phi3);
settable(t4,1,phx);
settable(t5,4,phi5);
settable(t8,1,phx);
settable(t9,8,phi9);
settable(t10,1,phx);
settable(t11,1,phy);
settable(t12,4,rec);
/* INITIALIZE VARIABLES */
if( dpwrf < 4095 )
{ printf("reset dpwrf=4095 and recalibrate C13 90 degree pulse");
psg_abort(1); }
/* maximum fine power for pwC pulses */
rf0 = 4095.0;
setautocal(); /* activate auto-calibration */
if (autocal[0] == 'n')
{
/* offC3 - 180 degree pulse on Ca, null at CO 118ppm away */
pwC3a = getval("pwC3a");
rf3 = (compC*4095.0*pwC*2.0)/pwC3a;
rf3 = (int) (rf3 + 0.5);
/* 90 degree one-lobe sinc pulse on CO, null at Ca 118ppm away */
pwC6 = getval("pwC6");
rf6 = (compC*4095.0*pwC*1.69)/pwC6; /* needs 1.69 times more */
rf6 = (int) (rf6 + 0.5); /* power than a square pulse */
/* 180 degree one-lobe sinc pulse on CO, null at Ca 118ppm away */
pwC8 = getval("pwC8");
rf8 = (compC*4095.0*pwC*2.0*1.65)/pwC8; /* needs 1.65 times more */
rf8 = (int) (rf8 + 0.5); /* power than a square pulse */
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /* needs 1.69 times more */
tpwrs = (int) (tpwrs); /* power than a square pulse */
/* power level and pulse time for WALTZ 1H decoupling */
pwHd = 1/(4.0 * waltzB1) ;
tpwrd = tpwr - 20.0*log10(pwHd/(compH*pw));
tpwrd = (int) (tpwrd + 0.5);
}
else /* if autocal = 'y'(yes), 'q'(quiet), 'r'(read) or 's'(semi) */
{
if(FIRST_FID) /* make shapes */
{
ppm = getval("dfrq");
bw = 118.0*ppm; ofs = -bw;
offC3 = pbox_make("offC3", "square180n", bw, ofs, compC*pwC, pwClvl);
offC6 = pbox_make("offC6", "sinc90n", bw, 0.0, compC*pwC, pwClvl);
offC8 = pbox_make("offC8", "sinc180n", bw, 0.0, compC*pwC, pwClvl);
H2Osinc = pbox_Rsh("H2Osinc", "sinc90", pwHs, 0.0, compH*pw, tpwr);
wz16 = pbox_Dcal("WALTZ16", 2.8*waltzB1, 0.0, compH*pw, tpwr);
ofs_check(H1ofs, C13ofs, N15ofs, H2ofs);
}
pwC3a = offC3.pw; rf3 = offC3.pwrf; /* set up parameters */
pwC6 = offC6.pw; rf6 = offC6.pwrf;
pwC8 = offC8.pw; rf8 = offC8.pwrf;
pwHs = H2Osinc.pw; tpwrs = H2Osinc.pwr-1.0; /* 1dB correction applied */
tpwrd = wz16.pwr; pwHd = 1.0/wz16.dmf;
}
if (tpwrsf < 4095.0) tpwrs = tpwrs + 6.0;
/* the pwC3 pulse at the middle of t1 */
if ((ni2 > 0.0) && (ni == 1.0)) ni = 0.0;
if (pwC3a > 2.0*pwN) pwZ = pwC3a; else pwZ = 2.0*pwN;
if ((pwC3==0.0) && (pwC3a>2.0*pwN)) pwZ1=pwC3a-2.0*pwN; else pwZ1=0.0;
if ( ni > 1 ) pwC3 = pwC3a;
if ( pwC3 > 0 ) phshift3 = 48.0;
else phshift3 = 0.0;
/* dipsi-3 decoupling on COCO */
p_trim = 1/(4*5000*(sfrq/600.0)); /* 5 kHz trim pulse at 600MHz as per Bax */
p_d = (5.0)/(9.0*4.0*2800.0*(sfrq/600.0)); /* 2.8 kHz DIPSI-3 at 600MHz as per Bax*/
rftrim = (compC*4095.0*pwC)/p_trim;
rftrim = (int)(rftrim+0.5);
rfd = (compC*4095.0*pwC*5.0)/(p_d*9.0);
rfd = (int) (rfd + 0.5);
ncyc = ((COmix - 0.002)/51.8/4/p_d);
ncyc = (int) (ncyc + 0.5);
initval(ncyc,v9);
/* CHECK VALIDITY OF PARAMETER RANGES */
if ( 0.5*ni2*1/(sw2) > timeTN - WFG3_START_DELAY)
{ printf(" ni2 is too big. Make ni2 equal to %d or less.\n",
((int)((timeTN - WFG3_START_DELAY)*2.0*sw2))); psg_abort(1);}
if ( dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y' )
{ printf("incorrect dec1 decoupler flags! Should be 'nnn' "); psg_abort(1);}
if ( dm2[A] == 'y' || dm2[B] == 'y' )
{ printf("incorrect dec2 decoupler flags! Should be 'nny' "); psg_abort(1);}
if ( dpwr2 > 50 )
{ printf("dpwr2 too large! recheck value "); psg_abort(1);}
if ( pw > 50.0e-6 )
{ printf(" pw too long ! recheck value "); psg_abort(1);}
if ( (pwN > 100.0e-6) && (ni>1 || ni2>1))
{ printf(" pwN too long! recheck value "); psg_abort(1);}
if ( TROSY[A] == 'y')
{ printf(" TROSY option is not implemented"); psg_abort(1);}
/* PHASES AND INCREMENTED TIMES */
/* Phase incrementation for hypercomplex 2D data, States-Haberkorn element */
if (phase1 == 2) tsadd(t3,1,4);
if (phase2 == 2)
{tsadd(t10,2,4); icosel = +1;}
else
icosel = -1;
/* Set up f1180 */
tau1 = d2;
if((f1180[A] == 'y') && (ni > 1.0))
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
/* Set up f2180 */
tau2 = d3;
if((f2180[A] == 'y') && (ni2 > 1.0))
{ tau2 += ( 1.0 / (2.0*sw2) ); if(tau2 < 0.2e-6) tau2 = 0.0; }
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{ tsadd(t3,2,4); tsadd(t12,2,4); }
if( ix == 1) d3_init = d3;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2)
{ tsadd(t8,2,4); tsadd(t12,2,4); }
/* BEGIN PULSE SEQUENCE */
status(A);
delay(d1);
rcvroff();
obspower(tpwr);
decpower(pwClvl);
dec2power(pwNlvl);
decpwrf(rf0);
obsoffset(tof);
txphase(zero);
delay(1.0e-5);
dec2rgpulse(pwN, zero, 0.0, 0.0); /*destroy N15 and C13 magnetization*/
decrgpulse(pwC, zero, 0.0, 0.0);
zgradpulse(gzlvl0, 0.5e-3);
delay(1.0e-4);
dec2rgpulse(pwN, one, 0.0, 0.0);
decrgpulse(pwC, zero, 0.0, 0.0);
zgradpulse(0.7*gzlvl0, 0.5e-3);
delay(5.0e-4);
rgpulse(pw,zero,0.0,0.0); /* 1H pulse excitation */
dec2phase(zero);
zgradpulse(gzlvl0, gt0);
delay(lambda - gt0);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
txphase(one);
zgradpulse(gzlvl0, gt0);
delay(lambda - gt0);
rgpulse(pw, one, 0.0, 0.0);
txphase(zero);
obspower(tpwrs);
if (tpwrsf<4095.0) obspwrf(tpwrsf);
shaped_pulse("H2Osinc", pwHs, zero, 5.0e-4, 0.0);
obspower(tpwrd);
if (tpwrsf<4095.0) obspwrf(4095.0);
zgradpulse(gzlvl3, gt3);
delay(2.0e-4);
dec2rgpulse(pwN, zero, 0.0, 0.0);
txphase(one);
delay(kappa - pwHd - 2.0e-6 - PRG_START_DELAY);
rgpulse(pwHd,one,0.0,0.0);
txphase(zero);
delay(2.0e-6);
obsprgon("waltz16", pwHd, 90.0); /* PRG_START_DELAY */
xmtron();
decphase(zero);
dec2phase(zero);
decpwrf(rf8);
delay(timeTN - kappa - WFG3_START_DELAY);
/* WFG3_START_DELAY */
sim3shaped_pulse("", "offC8", "", 0.0, pwC8, 2.0*pwN, zero, zero, zero,
0.0, 0.0);
decphase(t3);
decpwrf(rf6);
delay(timeTN);
dec2rgpulse(pwN, zero, 0.0, 0.0);
xmtroff();
obsprgoff();
rgpulse(pwHd,three,2.0e-6,0.0);
zgradpulse(gzlvl3, gt3);
delay(2.0e-4);
/***************************************************************/
/* The sequence is different from here with respect to ghn_co **/
/***************************************************************/
rgpulse(pwHd,one,2.0e-6,0.0); /* H1 decoupler is turned on */
txphase(zero);
delay(2.0e-6);
obsprgon("waltz16", pwHd, 90.0);
xmtron();
decshaped_pulse("offC6", pwC6, t3, 0.0, 0.0);
decphase(zero);
/* Refocus CO, evolve CO, spinlock CO and defocus CO */
delay(timeTN - tau1/2 - 0.6*pwC6 - WFG3_START_DELAY);
decpwrf(rf8);
sim3shaped_pulse("", "offC8","",0.0,pwC8, 2.0*pwN, zero,zero,zero,0.0,0.0);
decpwrf(rf3);
delay(timeTN - WFG3_STOP_DELAY - WFG_START_DELAY - pwC3a/2);
decshaped_pulse("offC3",pwC3a,zero,0.0,0.0);
if (tau1 > 0)
delay(tau1/2 - WFG_STOP_DELAY - pwC3a/2 - 2.0e-6);
else
delay(tau1/2);
/*******DO SPINLOCK ********/
decpwrf(rftrim);
decrgpulse(0.002,zero,2.0e-6,0.0);
decpwrf(rfd);
starthardloop(v9);
decrgpulse(6.4*p_d,zero,0.0,0.0);
decrgpulse(8.2*p_d,two,0.0,0.0);
decrgpulse(5.8*p_d,zero,0.0,0.0);
decrgpulse(5.7*p_d,two,0.0,0.0);
decrgpulse(0.6*p_d,zero,0.0,0.0);
decrgpulse(4.9*p_d,two,0.0,0.0);
decrgpulse(7.5*p_d,zero,0.0,0.0);
decrgpulse(5.3*p_d,two,0.0,0.0);
decrgpulse(7.4*p_d,zero,0.0,0.0);
decrgpulse(6.4*p_d,two,0.0,0.0);
decrgpulse(8.2*p_d,zero,0.0,0.0);
decrgpulse(5.8*p_d,two,0.0,0.0);
decrgpulse(5.7*p_d,zero,0.0,0.0);
decrgpulse(0.6*p_d,two,0.0,0.0);
decrgpulse(4.9*p_d,zero,0.0,0.0);
decrgpulse(7.5*p_d,two,0.0,0.0);
decrgpulse(5.3*p_d,zero,0.0,0.0);
decrgpulse(7.4*p_d,two,0.0,0.0);
decrgpulse(6.4*p_d,two,0.0,0.0);
decrgpulse(8.2*p_d,zero,0.0,0.0);
decrgpulse(5.8*p_d,two,0.0,0.0);
decrgpulse(5.7*p_d,zero,0.0,0.0);
decrgpulse(0.6*p_d,two,0.0,0.0);
decrgpulse(4.9*p_d,zero,0.0,0.0);
decrgpulse(7.5*p_d,two,0.0,0.0);
decrgpulse(5.3*p_d,zero,0.0,0.0);
decrgpulse(7.4*p_d,two,0.0,0.0);
decrgpulse(6.4*p_d,zero,0.0,0.0);
decrgpulse(8.2*p_d,two,0.0,0.0);
decrgpulse(5.8*p_d,zero,0.0,0.0);
decrgpulse(5.7*p_d,two,0.0,0.0);
decrgpulse(0.6*p_d,zero,0.0,0.0);
decrgpulse(4.9*p_d,two,0.0,0.0);
decrgpulse(7.5*p_d,zero,0.0,0.0);
decrgpulse(5.3*p_d,two,0.0,0.0);
decrgpulse(7.4*p_d,zero,0.0,0.0);
endhardloop();
decpwrf(4095.0);
/* End of spinlock */
delay(timeTN - WFG3_START_DELAY);
decpwrf(rf8);
sim3shaped_pulse("","offC8","",0.0,pwC8,2*pwN,zero,zero,zero,0.0,0.0);
decpwrf(rf6);
delay(timeTN - WFG3_STOP_DELAY);
/***************************************************************/
/* The sequence is same as ghn_co from this point ********/
/***************************************************************/
decshaped_pulse("offC6", pwC6, t5, 0.0, 0.0);
/* xxxxxxxxxxxxxxxxxx OPTIONS FOR N15 EVOLUTION xxxxxxxxxxxxxxxxxxxxx */
dec2phase(t8);
zgradpulse(gzlvl4, gt4);
txphase(one);
dcplrphase(zero);
delay(2.0e-4);
dec2rgpulse(pwN, t8, 0.0, 0.0);
decphase(zero);
dec2phase(t9);
decpwrf(rf8);
delay(timeTN - WFG3_START_DELAY - tau2);
/* WFG3_START_DELAY */
sim3shaped_pulse("", "offC8", "", 0.0, pwC8, 2.0*pwN, zero, zero, t9, 0.0, 0.0);
dec2phase(t10);
decpwrf(rf3);
if (tau2 > kappa)
{
delay(timeTN - pwC3a - WFG_START_DELAY); /* WFG_START_DELAY */
decshaped_pulse("offC3", pwC3a, zero, 0.0, 0.0);
delay(tau2 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6);
xmtroff();
obsprgoff(); /* PRG_STOP_DELAY */
rgpulse(pwHd,three,2.0e-6,0.0);
txphase(t4);
delay(kappa - gt1 - 2.0*GRADIENT_DELAY - 1.0e-4);
if (mag_flg[A]=='y') magradpulse(gzcal*gzlvl1, gt1);
else zgradpulse(gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
obspower(tpwr); /* POWER_DELAY */
delay(1.0e-4 - POWER_DELAY);
}
else if (tau2 > (kappa - pwC3a - WFG_START_DELAY))
{
delay(timeTN + tau2 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6);
xmtroff();
obsprgoff(); /* PRG_STOP_DELAY */
rgpulse(pwHd,three,2.0e-6,0.0);
txphase(t4); /* WFG_START_DELAY */
decshaped_pulse("offC3", pwC3a, zero, 0.0, 0.0);
delay(kappa -pwC3a -WFG_START_DELAY -gt1 -2.0*GRADIENT_DELAY -1.0e-4);
if (mag_flg[A]=='y') magradpulse(gzcal*gzlvl1, gt1);
else zgradpulse(gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
obspower(tpwr); /* POWER_DELAY */
delay(1.0e-4 - POWER_DELAY);
}
else if (tau2 > gt1 + 2.0*GRADIENT_DELAY + 1.0e-4)
{
delay(timeTN + tau2 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6);
xmtroff();
obsprgoff(); /* PRG_STOP_DELAY */
rgpulse(pwHd,three,2.0e-6,0.0);
txphase(t4);
delay(kappa - tau2 - pwC3a - WFG_START_DELAY); /* WFG_START_DELAY */
decshaped_pulse("offC3", pwC3a, zero, 0.0, 0.0);
delay(tau2 - gt1 - 2.0*GRADIENT_DELAY - 1.0e-4);
if (mag_flg[A]=='y') magradpulse(gzcal*gzlvl1, gt1);
else zgradpulse(gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
obspower(tpwr); /* POWER_DELAY */
delay(1.0e-4 - POWER_DELAY);
}
else
{
delay(timeTN + tau2 - kappa - PRG_STOP_DELAY - pwHd - 2.0e-6);
xmtroff();
obsprgoff(); /* PRG_STOP_DELAY */
rgpulse(pwHd,three,2.0e-6,0.0);
txphase(t4);
delay(kappa-tau2-pwC3a-WFG_START_DELAY-gt1-2.0*GRADIENT_DELAY-1.0e-4);
if (mag_flg[A]=='y') magradpulse(gzcal*gzlvl1, gt1);
else zgradpulse(gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
obspower(tpwr); /* POWER_DELAY */
delay(1.0e-4 - POWER_DELAY); /* WFG_START_DELAY */
decshaped_pulse("offC3", pwC3a, zero, 0.0, 0.0);
delay(tau2);
}
/* xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx */
sim3pulse(pw, 0.0, pwN, t4, zero, t10, 0.0, 0.0);
txphase(zero);
dec2phase(zero);
zgradpulse(gzlvl5, gt5);
delay(lambda - 1.3*pwN - gt5);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
txphase(one);
dec2phase(t11);
delay(lambda - 1.3*pwN - gt5);
sim3pulse(pw, 0.0, pwN, one, zero, t11, 0.0, 0.0);
txphase(zero);
dec2phase(zero);
zgradpulse(gzlvl6, gt5);
delay(lambda - 1.3*pwN - gt5);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
dec2phase(t10);
zgradpulse(gzlvl6, gt5);
delay(lambda - 0.65*pwN - gt5);
rgpulse(pw, zero, 0.0, 0.0);
delay((gt1/10.0) + 1.0e-4 +gstab - 0.5*pw + 2.0*GRADIENT_DELAY + POWER_DELAY);
rgpulse(2.0*pw, zero, 0.0,0.0);
dec2power(dpwr2); /* POWER_DELAY */
if (mag_flg[A] == 'y') magradpulse(icosel*gzcal*gzlvl2, gt1/10.0);
else zgradpulse(icosel*gzlvl2, gt1/10.0); /* 2.0*GRADIENT_DELAY */
delay(gstab);
rcvron();
statusdelay(C,1.0e-4);
setreceiver(t12);
}
pulsesequence()
{
char
shname1[MAXSTR],
f1180[MAXSTR],
f2180[MAXSTR],
SE_flg[MAXSTR];
int icosel = 0,
t1_counter,
t2_counter,
ni2 = getval("ni2"),
phase;
double d2_init=0.0,
d3_init=0.0,
pwS1,pwS2,pwS3,pwS4,pwS5,pwS6,pwS7,pwS8,
lambda = getval("lambda"),
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5"),
gzlvl6 = getval("gzlvl6"),
gt1 = getval("gt1"),
gt3 = getval("gt3"),
gt4 = getval("gt4"),
gt5 = getval("gt5"),
gt6 = getval("gt6"),
gstab = getval("gstab"),
shlvl1 = getval("shlvl1"),
shpw1 = getval("shpw1"),
pwClvl = getval("pwClvl"),
pwNlvl = getval("pwNlvl"),
pwN = getval("pwN"),
d2 = getval("d2"),
timeTN = getval("timeTN"),
Delta,
tauNCO = getval("tauNCO"),
tauC = getval("tauC"),
tau1 = getval("tau1"),
tau2 = getval("tau2"),
taunh = getval("taunh");
getstr("shname1", shname1);
getstr("SE_flg",SE_flg);
getstr("f1180",f1180);
getstr("f2180",f2180);
phase = (int) (getval("phase") + 0.5);
settable(t1,2,phi1);
settable(t2,1,phi2);
settable(t3,4,phi3);
settable(t4,8,phi4);
settable(t10,1,phi10);
settable(t12,8,phi12);
settable(t13,8,phi13);
/* INITIALIZE VARIABLES */
Delta = timeTN-tauNCO;
pwS1 = c13pulsepw("ca", "co", "square", 90.0);
pwS2 = c13pulsepw("ca", "co", "square", 180.0);
pwS3 = c13pulsepw("co", "ca", "sinc", 180.0);
pwS7 = c13pulsepw("co", "ca", "sinc", 90.0);
pwS8 = c_shapedpw("reburp",60.0 ,-135.0,zero, 0.0, 0.0);
pwS4 = h_shapedpw("eburp2",4.0,3.5,zero, 0.0, 0.0);
pwS6 = h_shapedpw("reburp",4.0,3.5,zero, 0.0, 0.0);
pwS5 = h_shapedpw("pc9f",4.0,3.5,zero, 2.0e-6, 0.0);
if (SE_flg[0] == 'y')
{
if ( ni2*1/(sw2)/2.0 > (timeTN-Delta-pwS3-pwS4))
{ printf(" ni2 is too big. Make ni2 equal to %d or less.\n",
((int)((timeTN-Delta-pwS3-pwS4)*2.0*sw2))); psg_abort(1);}
}
else
{
if ( ni2*1/(sw2)/2.0 > (timeTN-Delta-pwS3))
{ printf(" ni2 is too big. Make ni2 equal to %d or less.\n",
((int)((timeTN-Delta-pwS3)*2.0*sw2))); psg_abort(1);}
}
if (phase == 1) ;
if (phase == 2) {tsadd(t1,1,4);}
if (SE_flg[0] =='y')
{
if (phase2 == 2) {tsadd(t10,2,4); icosel = +1;}
else icosel = -1;
}
else
{
if (phase2 == 2) {tsadd(t3,1,4); icosel = 1;}
}
tau1 = d2;
if((f1180[A] == 'y') )
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
tau1 = tau1;
tau2 = d3;
if((f2180[A] == 'y') )
{ tau2 += ( 1.0 / (2.0*sw2) ); if(tau2 < 0.2e-6) tau2 = 0.0; }
tau2 = tau2;
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{ tsadd(t1,2,4); tsadd(t12,2,4); tsadd(t13,2,4); }
if( ix == 1) d3_init = d3;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2)
{ tsadd(t3,2,4); tsadd(t12,2,4); tsadd(t13,2,4); }
status(A);
decpower(pwClvl);
dec2power(pwNlvl);
set_c13offset("co");
zgradpulse(gzlvl6, gt6);
delay(1.0e-4);
delay(d1-gt6);
lk_hold();
rcvroff();
h_shapedpulse("pc9f",4.0,3.5,zero, 2.0e-6, 0.0);
delay(lambda-pwS5*0.5-pwS6*0.4);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, one, zero, zero, 0.0, 0.0);
delay(lambda-pwS5*0.5-pwS6*0.4);
h_shapedpulse("pc9f_",4.0,3.5,one, 2.0e-6, 0.0);
obspower(shlvl1);
/**************************************************************************/
/* xxxxxxxxxxxxxxxxxxxxxx N-> CA transfer xxxxxxxxxxxxxxxxxx */
/**************************************************************************/
set_c13offset("ca");
dec2rgpulse(pwN,zero,0.0,0.0);
delay(timeTN);
sim3_c13pulse("", "ca", "co", "square", "", 0.0, 180.0, 2.0*pwN,
zero, zero, zero, 2.0e-6, 2.0e-6);
delay(Delta);
c13pulse("co", "ca", "sinc", 180.0, zero, 0.0, 0.0);
delay(timeTN-Delta-pwS3-taunh*0.5-shpw1);
shaped_pulse(shname1,shpw1,two,0.0,0.0);
delay(taunh*0.5);
dec2rgpulse(pwN,zero,0.0,0.0);
shaped_pulse(shname1,shpw1,zero,0.0,0.0);
/**************************************************************************/
/* xxxxxxxxxxxxxxxxxxxxxx CA->CO TRANSFER xxxxxxxxxxxxxxxxxx */
/**************************************************************************/
set_c13offset("ca");
c13pulse("ca", "co", "square", 90.0, t2, 2.0e-6, 0.0);
/*
initval(0.0, v2);
decstepsize(1.0);
dcplrphase(v2);
*/
zgradpulse(gzlvl4, gt4);
delay(tauC*0.5-gt4);
c_shapedpulse2("isnob5",20.0,0.0,"isnob5",20.0,119.0,zero,0.0,0.0);
zgradpulse(gzlvl4, gt4);
delay(tauC-gt4);
c_shapedpulse2("isnob5",20.0,0.0,"isnob5",20.0,119.0,two,0.0,0.0);
delay(tauC*0.5);
c13pulse("ca", "co", "square", 90.0, one, 0.0, 0.0);
zgradpulse(gzlvl3, gt3*3.5);
delay(1.0e-4);
/**************************************************************************/
/* xxxxxxxxxxxxxxxxxxxxxx 13CO EVOLUTION xxxxxxxxxxxxxxxxxx */
/**************************************************************************/
set_c13offset("co");
c13pulse("co", "ca", "sinc", 90.0, t1, 2.0e-6, 0.0);
delay(tau1*0.5);
sim3_c13pulse(shname1, "ca", "co", "square", "",shpw1, 180.0, 2.0*pwN,
zero, zero, zero, 0.0, 0.0);
delay(tau1*0.5);
c13pulse("co", "ca", "sinc", 180.0, zero, 0.0, 0.0);
sim3_c13pulse(shname1, "ca", "co", "square", "",shpw1, 180.0, 0.0,
two, zero, zero, 0.0, 0.0);
if (pwN*2.0 > pwS2) delay(pwN*2.0-pwS2);
c13pulse("co", "ca", "sinc", 90.0, t4, 0.0, 0.0);
/***************************************************************************/
/* CA->CO transfer */
/***************************************************************************/
set_c13offset("ca");
c13pulse("ca", "co", "square", 90.0, zero, 2.0e-6, 0.0);
initval(0.0, v2);
decstepsize(1.0);
dcplrphase(v2);
zgradpulse(gzlvl3, gt3*2.0);
delay(tauC*0.5-gt3*2.0);
c_shapedpulse2("isnob5",20.0,0.0,"isnob5",20.0,119.0,two,0.0,0.0);
zgradpulse(gzlvl3, gt3*2.0);
delay(tauC-gt3*2.0);
c_shapedpulse2("isnob5",20.0,0.0,"isnob5",20.0,119.0,zero,0.0,0.0);
delay(tauC*0.5);
c13pulse("ca", "co", "square", 90.0, one, 0.0, 0.0);
/* dcplrphase(v2); */
/**************************************************************************/
obspower(shlvl1);
shaped_pulse(shname1,shpw1,zero,0.0,0.0);
dec2rgpulse(pwN,t3,0.0,0.0);
delay(tau2*0.5+taunh*0.5);
shaped_pulse(shname1,shpw1,two,0.0,0.0);
delay(timeTN-shpw1-taunh*0.5-gt1-1.0e-4);
zgradpulse(-gzlvl1, gt1);
delay(1.0e-4);
sim3_c13pulse("", "ca", "co", "square", "", 0.0, 180.0, 2.0*pwN,
zero, zero, zero, 2.0e-6, 2.0e-6);
if (SE_flg[0] == 'y')
{
delay(Delta);
c13pulse("co", "ca", "sinc", 180.0, zero, 0.0, 0.0);
delay(timeTN-tau2*0.5-pwS4-Delta-pwS3);
h_shapedpulse("eburp2",4.0,3.5,zero, 2.0e-6, 0.0);
dec2rgpulse(pwN, t10, 0.0, 0.0);
}
else
{
delay(Delta);
c13pulse("co", "ca", "sinc", 180.0, zero, 0.0, 0.0);
delay(timeTN-tau2*0.5-Delta-pwS3);
dec2rgpulse(pwN, zero, 0.0, 0.0);
}
/**************************************************************************/
if (SE_flg[0] == 'y')
{
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5);
dec2rgpulse(pwN, one, 0.0, 0.0);
h_shapedpulse("eburp2_",4.0,3.5,one, 0.0, 0.0);
txphase(zero);
dec2phase(zero);
delay(lambda-pwS4*0.5-pwS6*0.4);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
dec2phase(t10);
delay(lambda-pwS4*0.5-pwS6*0.4);
h_shapedpulse("eburp2",4.0,3.5,zero, 0.0, 0.0);
delay((gt1/10.0) + 1.0e-4 +gstab + 2.0*GRADIENT_DELAY + POWER_DELAY);
h_shapedpulse("reburp",4.0,3.5,zero, 0.0, 0.0);
zgradpulse(icosel*gzlvl2, gt1/10.0); /* 2.0*GRADIENT_DELAY */
delay(gstab);
}
else
{
h_shapedpulse("eburp2",4.0,3.5,zero, 2.0e-6, 0.0);
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5-POWER_DELAY-1.0e-4);
}
dec2power(dpwr2); /* POWER_DELAY */
lk_sample();
if (SE_flg[0] == 'y')
setreceiver(t13);
else
setreceiver(t12);
rcvron();
statusdelay(C,1.0e-4 );
}
pulsesequence()
{
char
shname1[MAXSTR],
f1180[MAXSTR],
f2180[MAXSTR],
SE_flg[MAXSTR],
href_flg[MAXSTR];
int icosel = 0,
t1_counter,
t2_counter,
ni2 = getval("ni2"),
phase;
double d2_init=0.0,
d3_init=0.0,
pwS1,pwS2,pwS3,pwS4,pwS5,pwS6,
lambda = getval("lambda"),
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gzlvl3 = getval("gzlvl3"),
gzlvl5 = getval("gzlvl5"),
gzlvl6 = getval("gzlvl6"),
gt1 = getval("gt1"),
/* gt2 = getval("gt2"), */
gt3 = getval("gt3"),
gt5 = getval("gt5"),
gt6 = getval("gt6"),
gstab = getval("gstab"),
shlvl1 = getval("shlvl1"),
shpw1 = getval("shpw1"),
pwClvl = getval("pwClvl"),
pwNlvl = getval("pwNlvl"),
pwN = getval("pwN"),
dpwr2 = getval("dpwr2"),
d2 = getval("d2"),
timeTN = getval("timeTN"),
tau1 = getval("tau1"),
tau2 = getval("tau2"),
taunh = getval("taunh");
getstr("shname1", shname1);
getstr("SE_flg",SE_flg);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("href_flg",href_flg);
phase = (int) (getval("phase") + 0.5);
settable(t1,4,phi1);
settable(t3,4,phi3);
settable(t5,4,phi5);
settable(t10,1,phi10);
settable(t12,4,phi12);
settable(t13,4,phi13);
/* INITIALIZE VARIABLES */
pwS1 = c13pulsepw("co", "ca", "sinc", 90.0);
pwS2 = c13pulsepw("co", "ca", "sinc", 180.0);
pwS3 = c13pulsepw("ca", "co", "square", 180.0);
pwS4 = h_shapedpw("eburp2",4.0,3.5,zero, 0.0, 0.0);
pwS6 = h_shapedpw("reburp",4.0,3.5,zero, 0.0, 0.0);
pwS5 = h_shapedpw("pc9f",4.0,3.5,zero, 2.0e-6, 0.0);
if (SE_flg[0] == 'y')
{
if ( ni2*1/(sw2)/2.0 > (timeTN-pwS2*0.5-pwS4))
{ printf(" ni2 is too big. Make ni2 equal to %d or less.\n",
((int)((timeTN-pwS2*0.5-pwS4)*2.0*sw2))); psg_abort(1);}
}
else
{
if ( ni2*1/(sw2)/2.0 > (timeTN-pwS2*0.5))
{ printf(" ni2 is too big. Make ni2 equal to %d or less.\n",
((int)((timeTN-pwS2*0.5)*2.0*sw2))); psg_abort(1);}
}
if (phase == 1) ;
if (phase == 2) tsadd(t1,1,4);
if (SE_flg[0] =='y')
{
if (phase2 == 2) {tsadd(t10,2,4); icosel = +1;}
else icosel = -1;
}
else
{
if (phase2 == 2) {tsadd(t3,1,4);tsadd(t5,1,4); icosel=1;}
}
tau1 = d2;
if((f1180[A] == 'y') )
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
tau1 = tau1;
tau2 = d3;
if((f2180[A] == 'y') )
{ tau2 += ( 1.0 / (2.0*sw2) ); if(tau2 < 0.2e-6) tau2 = 0.0; }
tau2 = tau2;
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{ tsadd(t1,2,4); tsadd(t12,2,4); tsadd(t13,2,4); }
if( ix == 1) d3_init = d3;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2)
{ tsadd(t3,2,4); tsadd(t5,2,4);tsadd(t12,2,4); tsadd(t13,2,4); }
status(A);
rcvroff();
decpower(pwClvl);
decoffset(dof);
dec2power(pwNlvl);
dec2offset(dof2);
decpwrf(4095.0);
obsoffset(tof);
set_c13offset("co");
zgradpulse(gzlvl6, gt6);
delay(1.0e-4);
delay(d1-gt6);
lk_hold();
h_shapedpulse("pc9f",4.0,3.5,zero, 2.0e-6, 0.0);
delay(lambda-pwS5*0.5-pwS6*0.4);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, one, zero, zero, 0.0, 0.0);
delay(lambda-pwS5*0.5-pwS6*0.4);
h_shapedpulse("pc9f_",4.0,3.5,one, 0.0, 0.0);
obspower(shlvl1);
/**************************************************************************/
dec2rgpulse(pwN,zero,0.0,0.0);
zgradpulse(gzlvl3, gt3);
delay(timeTN-pwS2*0.5-gt3);
sim3_c13pulse("", "co", "ca", "sinc", "", 0.0, 180.0, 2.0*pwN,
zero, zero, zero, 2.0e-6, 2.0e-6);
if (href_flg[0] == 'y')
{
delay(timeTN-pwS2*0.5-taunh*0.5-shpw1);
shaped_pulse(shname1,shpw1,two,0.0,0.0);
zgradpulse(gzlvl3, gt3);
delay(taunh*0.5-gt3);
dec2rgpulse(pwN,zero,0.0,0.0);
}
else
{
delay(timeTN-pwS2*0.5-taunh*0.5);
zgradpulse(gzlvl3, gt3);
delay(taunh*0.5-gt3);
dec2rgpulse(pwN,one,0.0,0.0);
}
/**************************************************************************/
/* xxxxxxxxxxxxxxxxxxxxxx 13CO EVOLUTION xxxxxxxxxxxxxxxxxx */
/**************************************************************************/
c13pulse("co", "ca", "sinc", 90.0, t1, 2.0e-6, 0.0);
delay(tau1*0.5);
sim3_c13pulse(shname1, "ca", "co", "square", "", shpw1, 180.0, 2.0*pwN,
zero, zero, zero, 2.0e-6, 0.0);
delay(tau1*0.5);
c13pulse("co", "ca", "sinc", 180.0, zero, 0.0, 0.0);
sim3_c13pulse("", "ca", "co", "square", "", 0.0, 180.0, 0.0,
one, zero, zero, 2.0e-6, 0.0);
if (pwN*2.0 > pwS3) delay(pwN*2.0-pwS3);
c13pulse("co", "ca", "sinc", 90.0, zero, 0.0, 0.0);
/**************************************************************************/
obspower(tpwr);
if (href_flg[0] == 'y')
{
shaped_pulse(shname1,shpw1,two,0.0,0.0);
dec2rgpulse(pwN,t3,0.0,0.0);
}
else
dec2rgpulse(pwN,t5,0.0,0.0);
if (SE_flg[0] == 'y')
{
if (href_flg[0] == 'y')
{
delay(tau2*0.5);
c13pulse("ca", "co", "square", 180.0, zero, 0.0, 0.0);
delay(taunh*0.5-pwS3);
shaped_pulse(shname1,shpw1,two,0.0,0.0);
delay(timeTN-pwS2*0.5-shpw1-taunh*0.5-gt1-1.0e-4);
}
else
{
delay(tau2*0.5);
sim_c13pulse(shname1,"ca", "co", "square",shpw1, 180.0, two,zero, 0.0, 0.0);
if (shpw1 >= pwS3) delay(taunh*0.5-shpw1);
else delay(taunh*0.5-pwS3);
delay(timeTN-pwS2*0.5-taunh*0.5-gt1-1.0e-4);
}
zgradpulse(-gzlvl1, gt1);
delay(1.0e-4);
sim3_c13pulse("", "co", "ca", "sinc", "", 0.0, 180.0, 2.0*pwN,
zero, zero, zero, 2.0e-6, 2.0e-6);
delay(timeTN-pwS2*0.5-tau2*0.5-pwS4);
h_shapedpulse("eburp2",4.0,3.5,zero, 2.0e-6, 0.0);
dec2rgpulse(pwN, t10, 0.0, 0.0);
}
else
{
if (href_flg[0] == 'y')
{
delay(tau2*0.5);
c13pulse("ca", "co", "square", 180.0, zero, 0.0, 0.0);
delay(taunh*0.5-pwS3);
shaped_pulse(shname1,shpw1,two,0.0,0.0);
delay(timeTN-pwS2*0.5-shpw1-taunh*0.5);
}
else
{
delay(tau2*0.5);
sim_c13pulse(shname1,"ca", "co", "square",shpw1, 180.0, two,zero, 0.0, 0.0);
if (shpw1 >= pwS3) delay(taunh*0.5-shpw1);
else delay(taunh*0.5-pwS3);
delay(timeTN-pwS2*0.5-taunh*0.5);
}
sim3_c13pulse("", "co", "ca", "sinc", "", 0.0, 180.0, 2.0*pwN,
zero, zero, zero, 2.0e-6, 2.0e-6);
delay(timeTN-pwS2*0.5-tau2*0.5);
dec2rgpulse(pwN, zero, 0.0, 0.0);
}
/**************************************************************************/
if (SE_flg[0] == 'y')
{
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5);
dec2rgpulse(pwN, one, 0.0, 0.0);
h_shapedpulse("eburp2_",4.0,3.5,one, 0.0, 0.0);
txphase(zero);
dec2phase(zero);
delay(lambda-pwS4*0.5-pwS6*0.4);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
dec2phase(t10);
delay(lambda-pwS4*0.5-pwS6*0.4);
h_shapedpulse("eburp2",4.0,3.5,zero, 0.0, 0.0);
delay((gt1/10.0) + 1.0e-4 +gstab + 2.0*GRADIENT_DELAY + POWER_DELAY);
h_shapedpulse("reburp",4.0,3.5,zero, 0.0, 0.0);
zgradpulse(icosel*gzlvl2, gt1/10.0); /* 2.0*GRADIENT_DELAY */
delay(gstab);
}
else
{
h_shapedpulse("eburp2",4.0,3.5,zero, 2.0e-6, 0.0);
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5);
h_sim3shapedpulse("reburp",4.0,3.5,0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.4 - gt5-POWER_DELAY-1.0e-4);
}
dec2power(dpwr2); /* POWER_DELAY */
lk_sample();
if (SE_flg[0] == 'y')
setreceiver(t13);
else
setreceiver(t12);
rcvron();
statusdelay(C,1.0e-4 );
}
pulsesequence()
{
char
shname1[MAXSTR],
f1180[MAXSTR],
f2180[MAXSTR],
n15_flg[MAXSTR],
CT_flg[MAXSTR];
int icosel,
t1_counter,
t2_counter,
ni2 = getval("ni2"),
phase;
double d2_init=0.0,
d3_init=0.0,
pwS1,pwS2,pwS3,pwS4,pwS5,pwS6,
kappa,
lambda = getval("lambda"),
CTdelay = getval("CTdelay"),
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5"),
gzlvl6 = getval("gzlvl6"),
gt1 = getval("gt1"),
gt4 = getval("gt4"),
gt5 = getval("gt5"),
gt6 = getval("gt6"),
gstab = getval("gstab"),
scale = getval("scale"),
sw1 = getval("sw1"),
tpwrsf = getval("tpwrsf"),
shlvl1,
shpw1 = getval("shpw1"),
pwC = getval("pwC"),
pwClvl = getval("pwClvl"),
pwNlvl = getval("pwNlvl"),
pwN = getval("pwN"),
dpwr2 = getval("dpwr2"),
d2 = getval("d2"),
t2a,t2b,halfT2,
t1a,t1b,halfT1,
shbw = getval("shbw"),
shofs = getval("shofs")-4.77,
timeTN = getval("timeTN"),
timeTN1 = getval("timeTN1"),
timeCN = getval("timeCN"),
tauC = getval("tauC"),
tauCC = getval("tauCC"),
tau1 = getval("tau1"),
tau2 = getval("tau2"),
taunh = getval("taunh");
getstr("shname1", shname1);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("n15_flg",n15_flg);
getstr("CT_flg",CT_flg);
phase = (int) (getval("phase") + 0.5);
settable(t1,2,phi1);
settable(t2,4,phi2);
settable(t3,1,phi3);
settable(t4,8,phi4);
settable(t5,1,phi5);
settable(t14,8,phi14);
settable(t24,8,phi24);
/* INITIALIZE VARIABLES */
kappa = 5.4e-3;
//shpw1 = pw*8.0;
shlvl1 = tpwr;
f1180[0] ='n';
f2180[0] ='n';
pwS1 = c13pulsepw("co", "ca", "sinc", 90.0);
pwS2 = c13pulsepw("co", "ca", "sinc", 180.0);
pwS3 = c13pulsepw("ca", "co", "square", 180.0);
pwS4 = h_shapedpw("eburp2",shbw,shofs,zero, 0.0, 0.0);
pwS6 = h_shapedpw("reburp",shbw,shofs,zero, 0.0, 0.0);
pwS5 = h_shapedpw("pc9f",shbw,shofs,zero, 2.0e-6, 0.0);
if (phase == 1) ;
if (phase == 2) {tsadd(t1,1,4);}
if ( phase2 == 2 )
{
tsadd ( t3,2,4 );
tsadd ( t5,2,4 );
icosel = +1;
}
else icosel = -1;
/* Set up f1180 */
tau1 = d2;
if((f1180[A] == 'y') && (ni > 1.0))
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
tau1 = tau1;
/* Set up f2180 */
tau2 = d3;
if((f2180[A] == 'y') && (ni2 > 1.0))
{ tau2 += ( 1.0 / (2.0*sw2) ); if(tau2 < 0.2e-6) tau2 = 0.0; }
tau2 = tau2;
/************************************************************/
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{ tsadd(t1,2,4);tsadd(t14,2,4); tsadd(t24,2,4); }
if( ix == 1) d3_init = d3;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2)
{ tsadd(t4,2,4); tsadd(t14,2,4); tsadd(t24,2,4); }
/************************************************************/
/* Set up CONSTANT/SEMI-CONSTANT time evolution in N15 */
/************************************************************/
if (ni2 > 1)
{
halfT2 = 0.5*(ni2-1)/sw2;
t2b = (double) t2_counter*((halfT2 - timeTN)/((double)(ni2-1)));
if( ix==1 && halfT2 - timeTN > 0 ) printf("SCT mode on, max ni2=%g\n",timeTN*sw2*2+1);
if(t2b < 0.0) t2b = 0.0;
t2a = timeTN - tau2*0.5 + t2b;
if(t2a < 0.2e-6) t2a = 0.0;
}
else
{
t2b = 0.0;
t2a = timeTN - tau2*0.5;
}
/************************************************************/
if (ni > 1)
{
halfT1 = 0.5*(ni-1)/sw1;
t1b = (double) t1_counter*((halfT1 - timeTN1)/((double)(ni-1)));
if(t1b < 0.0) t1b = 0.0;
t1a = timeTN1 - tau1*0.5 + t1b;
if(t1a < 0.2e-6) t1a = 0.0;
}
else
{
t1b = 0.0;
t1a = timeTN1 - tau1*0.5;
}
/************************************************************/
status(A);
rcvroff();
decpower(pwClvl);
decoffset(dof);
dec2power(pwNlvl);
dec2offset(dof2);
obspwrf(tpwrsf);
decpwrf(4095.0);
obsoffset(tof);
set_c13offset("co");
dec2rgpulse(pwN*2.0,zero,0.0,0.0);
zgradpulse(1.7*gzlvl4, gt4);
delay(1.0e-4);
lk_sample();
delay(d1-gt4);
lk_hold();
h_shapedpulse("pc9f",shbw,shofs,zero, 2.0e-6, 0.0);
delay(lambda-pwS5*0.5-pwS6*0.5);
h_sim3shapedpulse("reburp",shbw,shofs,0.0,2.0*pwN, one, zero, zero, 0.0, 0.0);
delay(lambda-pwS5*0.5-pwS6*0.5);
if(n15_flg[0]=='y') h_shapedpulse("pc9f_",shbw,shofs,three, 0.0, 0.0);
else h_shapedpulse("pc9f_",shbw,shofs,one, 0.0, 0.0);
shaped_pulse(shname1,shpw1,zero,0.0,0.0);
zgradpulse(2.3*gzlvl4, gt4);
delay(1.0e-4);
/**************************************************************************/
/*** N -> CO transfer *********************************/
/**************************************************************************/
dec2rgpulse(pwN,t1,0.0,0.0);
delay(tau1*0.5);
c13pulse("ca", "co", "square", 180.0, zero, 0.0, 0.0);
delay(taunh-pwS3);
shaped_pulse(shname1,shpw1,two,0.0,0.0);
delay(timeTN1-pwS2-taunh-shpw1);
c13pulse("co", "ca", "sinc", 180.0, zero, 0.0, 0.0);
delay (t1b);
dec2rgpulse (2.0*pwN, zero, 0.0, 0.0);
delay (t1a);
dec2rgpulse(pwN,zero,0.0,0.0);
/**************************************************************************/
/*** CO -> CA transfer *********************************/
/**************************************************************************/
c13pulse("co", "ca", "sinc", 90.0, t2, 2.0e-6, 0.0);
zgradpulse(-gzlvl4, gt4);
decphase(zero);
delay(tauC - gt4 - 0.5*10.933*pwC);
decrgpulse(pwC*158.0/90.0, zero, 0.0, 0.0);
decrgpulse(pwC*171.2/90.0, two, 0.0, 0.0);
decrgpulse(pwC*342.8/90.0, zero, 0.0, 0.0); /* Shaka 6 composite */
decrgpulse(pwC*145.5/90.0, two, 0.0, 0.0);
decrgpulse(pwC*81.2/90.0, zero, 0.0, 0.0);
decrgpulse(pwC*85.3/90.0, two, 0.0, 0.0);
zgradpulse(-gzlvl4, gt4);
decphase(one);
delay(tauC - gt4 - 0.5*10.933*pwC - WFG_START_DELAY - 2.0e-6);
c13pulse("co", "ca", "sinc", 90.0, one, 0.0, 0.0);
/**************************************************************************/
/*** CA -> N transfer *********************************/
/**************************************************************************/
set_c13offset("ca");
c13pulse("ca", "co", "square", 90.0, zero, 2.0e-6, 0.0);
delay(tauC);
c13pulse("co", "ca", "sinc", 180.0, zero, 0.0, 0.0);
delay(timeCN-tauC);
sim3_c13pulse("", "cab", "co", "square", "", 0.0, 180.0, 2.0*pwN, zero, zero, zero, 2.0e-6, 2.0e-6);
delay(timeCN);
c13pulse("co", "ca", "sinc", 180.0, zero, 0.0, 0.0);
c13pulse("ca", "co", "square", 90.0, one, 2.0e-6, 0.0);
/**************************************************************************/
/*** N -> CA back transfer *********************************/
/**************************************************************************/
dec2rgpulse(pwN,t4,0.0,0.0);
delay(tau2*0.5);
c13pulse("co", "ca", "sinc", 180.0, zero, 0.0, 0.0);
//delay(timeTN-pwS3-pwS2-gt1-1.0e-4);
delay(timeTN-pwS3-pwS2-gt1-1.0e-4-2.0*GRADIENT_DELAY-4*POWER_DELAY-4*PWRF_DELAY-(4/PI)*pwN);
zgradpulse(-gzlvl1, gt1);
delay(1.0e-4);
c13pulse("ca", "co", "square", 180.0, zero, 0.0, 0.0);
delay (t2b);
dec2rgpulse (2.0*pwN, zero, 0.0, 0.0);
delay (t2a);
/**************************************************************************/
delay(gt1/10.0+1.0e-4);
h_shapedpulse("eburp2_",shbw,shofs,t3, 2.0e-6, 0.0);
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.5-pwS4*scale- gt5);
h_sim3shapedpulse("reburp",shbw,shofs,0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
delay(lambda-pwS6*0.5-pwS4*scale- gt5);
h_shapedpulse("eburp2",shbw,shofs,zero, 0.0, 0.0);
delay(gt1/10.0+1.0e-4);
dec2rgpulse(pwN,one,0.0,0.0);
zgradpulse(gzlvl6, gt6);
txphase(zero);
delay(lambda-pwS6*0.5-gt6);
h_sim3shapedpulse("reburp",shbw,shofs,0.0,2.0*pwN, zero, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl6, gt6);
delay(lambda-pwS6*0.5-gt6);
dec2rgpulse(pwN,t5,0.0,0.0);
/**************************************************************************/
zgradpulse(-icosel*gzlvl2, gt1/10.0);
dec2power(dpwr2); /* POWER_DELAY */
lk_sample();
if (n15_flg[0] =='y')
{
setreceiver(t14);
}
else
{
setreceiver(t24);
}
rcvron();
statusdelay(C,1.0e-4 );
}
pulsesequence()
{
double gtE = getval("gtE"),
gzlvlE=getval("gzlvlE"),
selpwrPS = getval("selpwrPS"),
selpwPS = getval("selpwPS"),
gzlvlPS = getval("gzlvlPS"),
droppts=getval("droppts"),
gstab = getval("gstab");
char selshapePS[MAXSTR];
//synchronize gradients to srate for probetype='nano'
// Preserve gradient "area"
gtE = syncGradTime("gtE","gzlvlE",1.0);
gzlvlE = syncGradLvl("gtE","gzlvlE",1.0);
getstr("selshapePS",selshapePS);
settable(t1,4,ph1);
settable(t2,32,ph2);
settable(t3,8,ph3);
settable(t4,16,ph4);
getelem(t1,ct,v1);
getelem(t2,ct,v2);
getelem(t3,ct,v3);
getelem(t4,ct,v4);
assign(v4,oph);
assign(v1,v6);
/* BEGIN THE ACTUAL PULSE SEQUENCE */
status(A);
obspower(tpwr);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
if (satmode[0] == 'y')
{
if ((d1-satdly) > 0.02)
delay(d1-satdly);
else
delay(0.02);
satpulse(satdly,v6,rof1,rof1);
}
else
delay(d1);
if (getflag("wet"))
wet4(zero,one);
status(B);
obspower(tpwr);
rgpulse(pw, v1, rof1, rof2);
delay(d2/2.0);
delay((0.25/sw1) - gtE - gstab - 2*pw/PI - rof2 - 2*GRADIENT_DELAY);
zgradpulse(gzlvlE,gtE);
delay(gstab);
rgpulse(2*pw,v2,rof1,rof1);
delay(0.25/sw1);
delay(gstab);
zgradpulse(-1.0*gzlvlE,gtE);
delay(gstab);
obspower(selpwrPS);
rgradient('z',gzlvlPS);
shaped_pulse(selshapePS,selpwPS,v3,rof1,rof1);
rgradient('z',0.0);
obspower(tpwr);
delay(gstab);
zgradpulse(-2.0*gzlvlE,gtE);
delay(gstab - droppts/sw);
delay(d2/2.0);
status(C);
}
pulsesequence()
{
/* DECLARE VARIABLES */
char autocal[MAXSTR], /* auto-calibration flag */
fsat[MAXSTR],
fscuba[MAXSTR],
f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
ddseq[MAXSTR], /* deuterium decoupling sequence */
shp_sl[MAXSTR],
shcreb[MAXSTR], /* reburp shape for center of t1 period */
shcgcob[MAXSTR], /* g3 inversion at 154 ppm (350 us) */
shcgcoib[MAXSTR], /* g3 time inversion at 154 ppm (350 us) */
shca180[MAXSTR], /* Ca 180 [D/sq(3)] during 15N CT */
shco180[MAXSTR], /* Co 180 [D/sq(15)] during 15N CT */
sel_flg[MAXSTR], /* active/passive purging of undesired
component */
fCT[MAXSTR], /* Flag for constant time C13 evolution */
fc180[MAXSTR],
cal_sphase[MAXSTR],
shared_CT[MAXSTR],
nietl_flg[MAXSTR];
int phase, phase2, ni2, icosel,
t1_counter, /* used for states tppi in t1 */
t2_counter; /* used for states tppi in t2 */
double tau1, /* t1 delay */
tau2, /* t2 delay */
taua, /* ~ 1/4JNH = 2.25 ms */
del1, /* time for C'-N to refocus set to 0.5*24.0 ms */
bigTN, /* nitrogen T period */
bigTC, /* carbon T period */
zeta, /* delay for transfer from ca to cb = 3.5 ms */
tsatpwr, /* low level 1H trans.power for presat */
sw1, /* sweep width in f1 */
sw2, /* sweep width in f2 */
tauf, /* 1/2J NH value */
pw_sl, /* selective pulse on water */
phase_sl, /* phase on water */
tpwrsl, /* power for pw_sl */
at,
d_cgcob, /* power level for g3 pulses at 154 ppm */
d_creb, /* power level for reburp 180 at center of t1 */
pwcgcob, /* g3 ~ 35o us 180 pulse */
pwcreb, /* reburp ~ 400us 180 pulse */
pwD, /* 2H 90 pulse, about 125 us */
pwDlvl, /* 2H 90 pulse, about 125 us */
pwca180, /* Ca 180 during N CT at d_ca180 */
pwco180, /* Co 180 during N CT at d_co180 */
d_ca180,
d_co180,
compC = getval("compC"), /* C-13 RF calibration parameters */
pwC = getval("pwC"),
pwClvl = getval("pwClvl"),
pwN,
pwNlvl,
sphase,
pw_sl1,
tpwrsl1,
gstab,
gt1,
gt2,
gt3,
gt4,
gt5,
gt6,
gt7,
gt8,
gt9,
gt11,
gt13,
gt14,
gzlvl1,
gzlvl2,
gzlvl3,
gzlvl4,
gzlvl5,
gzlvl6,
gzlvl7,
gzlvl8,
gzlvl9,
gzlvl11,
gzlvl13,
gzlvl14;
/* variables commented out are already defined by the system */
/* LOAD VARIABLES */
getstr("autocal",autocal);
getstr("fsat",fsat);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("fscuba",fscuba);
getstr("ddseq",ddseq);
getstr("shp_sl",shp_sl);
getstr("sel_flg",sel_flg);
getstr("fCT",fCT);
getstr("fc180",fc180);
getstr("cal_sphase",cal_sphase);
getstr("shared_CT",shared_CT);
getstr("nietl_flg",nietl_flg);
taua = getval("taua");
del1 = getval("del1");
bigTN = getval("bigTN");
bigTC = getval("bigTC");
zeta = getval("zeta");
pwN = getval("pwN");
tpwr = getval("tpwr");
tsatpwr = getval("tsatpwr");
dpwr = getval("dpwr");
pwNlvl = getval("pwNlvl");
pwD = getval("pwD");
pwDlvl = getval("pwDlvl");
phase = (int) ( getval("phase") + 0.5);
phase2 = (int) ( getval("phase2") + 0.5);
sw1 = getval("sw1");
sw2 = getval("sw2");
ni2 = getval("ni2");
tauf = getval("tauf");
pw_sl = getval("pw_sl");
phase_sl = getval("phase_sl");
tpwrsl = getval("tpwrsl");
at = getval("at");
sphase = getval("sphase");
pw_sl1 = getval("pw_sl1");
tpwrsl1 = getval("tpwrsl1");
gstab = getval("gstab");
gt1 = getval("gt1");
if (getval("gt2") > 0) gt2=getval("gt2");
else gt2=gt1*0.1;
gt3 = getval("gt3");
gt4 = getval("gt4");
gt5 = getval("gt5");
gt6 = getval("gt6");
gt7 = getval("gt7");
gt8 = getval("gt8");
gt9 = getval("gt9");
gt11 = getval("gt11");
gt13 = getval("gt13");
gt14 = getval("gt14");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl3 = getval("gzlvl3");
gzlvl4 = getval("gzlvl4");
gzlvl5 = getval("gzlvl5");
gzlvl6 = getval("gzlvl6");
gzlvl7 = getval("gzlvl7");
gzlvl8 = getval("gzlvl8");
gzlvl9 = getval("gzlvl9");
gzlvl11 = getval("gzlvl11");
gzlvl13 = getval("gzlvl13");
gzlvl14 = getval("gzlvl14");
if(autocal[0]=='n')
{
getstr("shcgcob",shcgcob);
getstr("shcgcoib",shcgcoib);
getstr("shcreb",shcreb);
getstr("shca180",shca180);
getstr("shco180",shco180);
d_ca180 = getval("d_ca180");
d_co180 = getval("d_co180");
d_cgcob = getval("d_cgcob");
d_creb = getval("d_creb");
pwca180 = getval("pwca180");
pwco180 = getval("pwco180");
pwcgcob = getval("pwcgcob");
pwcreb = getval("pwcreb");
}
else
{
strcpy(shcgcob,"Pg3_107p");
strcpy(shcgcoib,"Pg3i_107p");
strcpy(shcreb,"Preb_on");
strcpy(shca180,"Phard_15p");
strcpy(shco180,"Phard_133p");
if (FIRST_FID)
{
cgcob = pbox(shcgcob, G3CGCOB, CAB180ps, dfrq, compC*pwC, pwClvl);
cgcoib = pbox(shcgcoib, G3CGCOBi, CAB180ps, dfrq, compC*pwC, pwClvl);
creb = pbox(shcreb, CREB180, CAB180ps, dfrq, compC*pwC, pwClvl);
ca180 = pbox(shca180, CA180, CA180ps, dfrq, compC*pwC, pwClvl);
co180 = pbox(shco180, CO180, CA180ps, dfrq, compC*pwC, pwClvl);
}
d_ca180 = ca180.pwr;
d_co180 = co180.pwr;
d_cgcob = cgcob.pwr;
d_creb = creb.pwr;
pwca180 = ca180.pw;
pwco180 = co180.pw;
pwcgcob = cgcob.pw;
pwcreb = creb.pw;
}
/* LOAD PHASE TABLE */
settable(t1,2,phi1);
settable(t2,4,phi2);
settable(t3,8,phi3);
settable(t4,1,phi4);
settable(t5,16,phi5);
settable(t6,8,phi6);
settable(t7,1,phi7);
settable(t8,16,rec);
/* CHECK VALIDITY OF PARAMETER RANGES */
if(shared_CT[A] == 'n')
if(bigTN - 0.5*(ni2 -1)/sw2 - POWER_DELAY < 0.2e-6)
{
text_error(" ni2 is too big\n");
text_error(" please set ni2 smaller or equal to %d\n",
(int) ((bigTN -POWER_DELAY)*sw2*2.0) +1 );
psg_abort(1);
}
if(fCT[A] == 'y')
if(bigTC - 0.5*(ni-1)/sw1 - WFG_STOP_DELAY - gt14 - 102.0e-6
- POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY - PRG_START_DELAY
- POWER_DELAY - WFG_START_DELAY - 4.0e-6 - pwcgcob - WFG_STOP_DELAY
- POWER_DELAY - 4.0e-6 < 0.2e-6) {
text_error("ni is too big\n");
text_error(" please set ni smaller or equal to %d\n",
(int) ((bigTC - WFG_STOP_DELAY - gt14 - 102.0e-6
- POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY - PRG_START_DELAY
- POWER_DELAY - WFG_START_DELAY - 4.0e-6 - pwcgcob - WFG_STOP_DELAY
- POWER_DELAY - 4.0e-6)*sw1*2.0) +1 );
psg_abort(1);
}
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y'))
{
text_error("incorrect dec1 decoupler flags! ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y'))
{
text_error("incorrect dec2 decoupler flags! Should be 'nnn' ");
psg_abort(1);
}
if( tsatpwr > 6 )
{
text_error("TSATPWR too large !!! ");
psg_abort(1);
}
if( dpwr > 46 )
{
text_error("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if( dpwr2 > 47 )
{
text_error("don't fry the probe, DPWR2 too large! ");
psg_abort(1);
}
if( pwClvl > 63 )
{
text_error("don't fry the probe, pwClvl too large! ");
psg_abort(1);
}
if( pwNlvl > 63 )
{
text_error("don't fry the probe, pwNlvl too large! ");
psg_abort(1);
}
if( pw > 200.0e-6 )
{
text_error("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwN > 200.0e-6 )
{
text_error("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
if( pwC > 200.0e-6 )
{
text_error("dont fry the probe, pwC too high ! ");
psg_abort(1);
}
if( f1180[A] != 'n' && f2180[A] != 'n' ) {
text_error("flags may be set wrong: set f1180=n and f2180=n for 3d\n");
psg_abort(1);
}
if(d_ca180 > 58)
{
text_error("dont fry the probe, d_ca180 too high ! ");
psg_abort(1);
}
if(d_co180 > 58)
{
text_error("dont fry the probe, d_ca180 too high ! ");
psg_abort(1);
}
if( gt1 > 15e-3 || gt2 > 15e-3 || gt3 > 15e-3
|| gt4 > 15e-3 || gt5 > 15e-3 || gt6 > 15e-3
|| gt7 > 15e-3 || gt8 > 15e-3 || gt9 > 15e-3
|| gt11 > 15e-3 || gt13 > 15e-3
|| gt14 > 15e-3)
{
text_error("gti values must be < 15e-3\n");
psg_abort(1);
}
if(tpwrsl > 25) {
text_error("tpwrsl must be less than 25\n");
psg_abort(1);
}
if(tpwrsl1 > 25) {
text_error("tpwrsl1 must be less than 25\n");
psg_abort(1);
}
if( dpwr3 > 50) {
text_error("dpwr3 too high\n");
psg_abort(1);
}
if( del1 > 0.1 ) {
text_error("too long del1\n");
psg_abort(1);
}
if( zeta > 0.1 ) {
text_error("too long zeta\n");
psg_abort(1);
}
if( bigTN > 0.1) {
text_error("too long bigTN\n");
psg_abort(1);
}
if( bigTC > 0.1) {
text_error("too long bigTC\n");
psg_abort(1);
}
if( pw_sl > 10e-3) {
text_error("too long pw_sl\n");
psg_abort(1);
}
if( pw_sl1 > 10e-3) {
text_error("too long pw_sl1\n");
psg_abort(1);
}
if( at > 0.1 && dm2[D] == 'y') {
text_error("too long at with dec2\n");
psg_abort(1);
}
if(pwDlvl > 59) {
text_error("pwDlvl is too high; <= 59\n");
psg_abort(1);
}
if(d_creb > 62) {
text_error("d_creb is too high; <= 62\n");
psg_abort(1);
}
if(d_cgcob > 60) {
text_error("d_cgcob is too high; <=60\n");
psg_abort(1);
}
if(cal_sphase[A] == 'y') {
text_error("Use only to calibrate sphase\n");
text_error("Set zeta to 600 us, gt11=gt13=0, fCT=y, fc180=n\n");
}
if(nietl_flg[A] == 'y' && sel_flg[A] == 'y') {
text_error("Both nietl_flg and sel_flg cannot by y\n");
psg_abort(1);
}
if (fCT[A] == 'n' && fc180[A] =='y' && ni > 1.0) {
text_error("must set fc180='n' to allow Calfa/Cbeta evolution (ni>1)\n");
psg_abort(1);
}
/* Phase incrementation for hypercomplex 2D data */
/* changed from 1 to 3; spect. rev. not needed */
if (phase == 2) { tsadd(t2,3,4); tsadd(t3,3,4); }
if (shared_CT[A] == 'n')
{
if (phase2 == 2) { tsadd(t7,2,4); icosel = 1; }
else icosel = -1;
}
else
{
if (phase2 == 2) { tsadd(t7,2,4); icosel = -1; }
else icosel = 1;
}
if (nietl_flg[A] == 'y') icosel = -1*icosel;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2 ;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2) {
tsadd(t2,2,4);
tsadd(t8,2,4);
}
if( ix == 1) d3_init = d3 ;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2) {
tsadd(t5,2,4);
tsadd(t8,2,4);
}
/* Set up f1180 tau1 = t1 */
tau1 = d2;
if(f1180[A] == 'y' && fCT[A] == 'y')
tau1 += ( 1.0 / (2.0*sw1) );
if(f1180[A] == 'y' && fCT[A] == 'n')
tau1 += (1.0 / (2.0*sw1) - 4.0/PI*pwC - POWER_DELAY
- 4.0e-6);
if(f1180[A] == 'n' && fCT[A] == 'n')
tau1 = (tau1 - 4.0/PI*pwC - POWER_DELAY
- 4.0e-6);
if(tau1 < 0.2e-6) tau1 = 4.0e-7;
tau1 = tau1/2.0;
/* Set up f2180 tau2 = t2 */
tau2 = d3;
if(f2180[A] == 'y') {
tau2 += ( 1.0 / (2.0*sw2) );
if(tau2 < 0.2e-6) tau2 = 0.2e-6;
}
tau2 = tau2/2.0;
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(tsatpwr); /* Set transmitter power for 1H presaturation */
decpower(pwClvl); /* Set Dec1 power to high power */
dec2power(pwNlvl); /* Set Dec2 power for 15N hard pulses */
dec3power(pwDlvl); /* Set Dec3 for 2H hard pulses */
/* Presaturation Period */
if (fsat[0] == 'y')
{
delay(2.0e-5);
rgpulse(d1,zero,0.0,2.0e-6);
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
delay(2.0e-5);
if (fscuba[0] == 'y')
{
delay(2.2e-2);
rgpulse(pw,zero,2.0e-6,0.0);
rgpulse(2*pw,one,2.0e-6,0.0);
rgpulse(pw,zero,2.0e-6,0.0);
delay(2.2e-2);
}
}
else
{
delay(d1);
}
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
txphase(zero);
dec2phase(zero);
delay(1.0e-5);
/* Begin Pulses */
status(B);
rcvroff();
lk_hold();
delay(20.0e-6);
rgpulse(pw,zero,0.0,0.0); /* 90 deg 1H pulse */
delay(0.2e-6);
zgradpulse(gzlvl5,gt5);
delay(2.0e-6);
delay(taua - gt5 - 2.2e-6); /* taua <= 1/4JNH */
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
dec2phase(t1); decphase(zero);
delay(taua - gt5 - 200.2e-6);
delay(0.2e-6);
zgradpulse(gzlvl5,gt5);
delay(200.0e-6);
if (sel_flg[A] == 'y')
{
rgpulse(pw,one,4.0e-6,0.0);
initval(1.0,v2);
obsstepsize(phase_sl);
xmtrphase(v2);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shp_sl,pw_sl,two,2.0e-6,0.0);
xmtrphase(zero);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
initval(1.0,v6);
dec2stepsize(45.0);
dcplr2phase(v6);
delay(0.2e-6);
zgradpulse(gzlvl3,gt3);
delay(200.0e-6);
dec2rgpulse(pwN,t1,0.0,0.0);
dcplr2phase(zero);
delay(1.34e-3 - SAPS_DELAY);
rgpulse(pw,zero,0.0,0.0);
rgpulse(2.0*pw,one,2.0e-6,0.0);
rgpulse(pw,zero,2.0e-6,0.0);
decpower(d_ca180);
dec2phase(zero);
delay(del1 - 1.34e-3 - 4.0*pw - 4.0e-6
- POWER_DELAY + WFG_START_DELAY + pwca180 + WFG_STOP_DELAY);
}
else
{
rgpulse(pw,three,4.0e-6,0.0);
initval(1.0,v2);
obsstepsize(phase_sl);
xmtrphase(v2);
/* shaped pulse */
obspower(tpwrsl);
shaped_pulse(shp_sl,pw_sl,zero,2.0e-6,0.0);
xmtrphase(zero);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
delay(0.2e-6);
zgradpulse(gzlvl3,gt3);
delay(200.0e-6);
dec2rgpulse(pwN,t1,0.0,0.0);
dec2phase(zero);
decpower(d_ca180);
delay(del1 - POWER_DELAY + WFG_START_DELAY
+ pwca180 + WFG_STOP_DELAY);
}
decphase(zero);
dec2rgpulse(2*pwN,zero,0.0,0.0);
decshaped_pulse(shca180,pwca180,zero,0.0,0.0);
dec2phase(one);
delay(del1);
dec2rgpulse(pwN,one,0.0,0.0);
decpower(pwClvl);
decphase(t2);
delay(0.2e-6);
zgradpulse(gzlvl4,gt4);
delay(200.0e-6);
dec2phase(t5);
/* Turn on D decoupling using the third decoupler */
dec3phase(one);
dec3power(pwDlvl);
dec3rgpulse(pwD,one,4.0e-6,0.0);
dec3phase(zero);
dec3power(dpwr3);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
/* Turn on D decoupling */
decrgpulse(pwC,t2,0.0,0.0);
delay(zeta
- PRG_STOP_DELAY - DELAY_BLANK - POWER_DELAY - 4.0e-6
- pwD
- gt11 - 102.0e-6 - POWER_DELAY - WFG_START_DELAY);
/* Turn off D decoupling */
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3blank();
dec3phase(three);
dec3power(pwDlvl);
dec3rgpulse(pwD,three,4.0e-6,0.0);
/* Turn off D decoupling */
decphase(zero);
delay(2.0e-6);
zgradpulse(gzlvl11,gt11);
delay(100.0e-6);
if (cal_sphase[A] == 'y')
{
decpower(pwClvl);
decshaped_pulse("hard",2.0*pwC,zero,4.0e-6,4.0e-6);
}
else
{
initval(1.0,v3);
decstepsize(sphase);
dcplrphase(v3);
decpower(d_creb);
decshaped_pulse(shcreb,pwcreb,zero,4.0e-6,4.0e-6);
dcplrphase(zero);
}
delay(2.0e-6);
zgradpulse(gzlvl11,gt11);
delay(100.0e-6);
/* Turn on D decoupling using the third decoupler */
dec3phase(one);
dec3power(pwDlvl);
dec3rgpulse(pwD,one,4.0e-6,0.0);
dec3phase(zero);
dec3power(dpwr3);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
/* Turn on D decoupling */
delay(zeta - WFG_STOP_DELAY - gt11 - 102.0e-6
- POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY - PRG_START_DELAY
- DELAY_BLANK - POWER_DELAY - 4.0e-6);
decpower(pwClvl);
decrgpulse(pwC,t3,4.0e-6,0.0);
if (fCT[A] == 'y')
{
delay(tau1);
decpower(d_cgcob);
decshaped_pulse(shcgcob,pwcgcob,zero,4.0e-6,0.0);
delay(bigTC - POWER_DELAY - WFG_START_DELAY - 4.0e-6
- pwcgcob - WFG_STOP_DELAY
- 102.0e-6 - gt14
- PRG_STOP_DELAY - DELAY_BLANK
- POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY - WFG_START_DELAY);
/* Turn off D decoupling */
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3blank();
dec3phase(three);
dec3power(pwDlvl);
dec3rgpulse(pwD,three,4.0e-6,0.0);
/* Turn off D decoupling */
delay(2.0e-6);
zgradpulse(gzlvl14,gt14);
delay(100.0e-6);
initval(1.0,v4);
decstepsize(sphase);
dcplrphase(v4);
decpower(d_creb);
decshaped_pulse(shcreb,pwcreb,zero,4.0e-6,4.0e-6);
dcplrphase(zero);
delay(2.0e-6);
zgradpulse(gzlvl14,gt14);
delay(100.0e-6);
/* Turn on D decoupling using the third decoupler */
dec3phase(one);
dec3power(pwDlvl);
dec3rgpulse(pwD,one,4.0e-6,0.0);
dec3phase(zero);
dec3power(dpwr3);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
/* Turn on D decoupling */
delay(bigTC - tau1 - WFG_STOP_DELAY - gt14
- 102.0e-6 - POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY
- PRG_START_DELAY - POWER_DELAY - WFG_START_DELAY
- 4.0e-6 - pwcgcob - WFG_STOP_DELAY - POWER_DELAY
- 4.0e-6);
decpower(d_cgcob);
decshaped_pulse(shcgcoib,pwcgcob,zero,4.0e-6,0.0);
decphase(t4);
}
else if(fCT[A] == 'n' && fc180[A] == 'n')
{
delay(tau1);
delay(tau1);
}
else if(fCT[A] == 'n' && fc180[A] == 'y')
{
initval(1.0,v4);
decstepsize(sphase);
dcplrphase(v4);
decpower(d_creb);
decshaped_pulse(shcreb,pwcreb,zero,4.0e-6,0.0);
dcplrphase(zero);
}
decpower(pwClvl);
decrgpulse(pwC,t4,4.0e-6,0.0);
delay(zeta - POWER_DELAY - 4.0e-6
- pwD - PRG_STOP_DELAY - DELAY_BLANK
- gt13
- 102.0e-6 - POWER_DELAY - WFG_START_DELAY);
/* Turn off D decoupling */
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3blank();
dec3power(pwDlvl);
dec3rgpulse(pwD,three,4.0e-6,0.0);
/* Turn off D decoupling */
delay(2.0e-6);
zgradpulse(gzlvl13,gt13);
delay(100.0e-6);
if (cal_sphase[A] == 'y')
{
decpower(pwClvl);
decshaped_pulse("hard",2.0*pwC,zero,4.0e-6,4.0e-6);
}
else
{
initval(1.0,v5);
decstepsize(sphase);
dcplrphase(v5);
decpower(d_creb);
decshaped_pulse(shcreb,pwcreb,zero,4.0e-6,4.0e-6);
dcplrphase(zero);
}
delay(2.0e-6);
zgradpulse(gzlvl13,gt13);
delay(100.0e-6);
/* Turn on D decoupling using the third decoupler */
dec3phase(one);
dec3power(pwDlvl);
dec3rgpulse(pwD,one,4.0e-6,0.0);
dec3phase(zero);
dec3power(dpwr3);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
/* Turn on D decoupling */
delay(zeta - WFG_STOP_DELAY - gt13 - 102.0e-6
- POWER_DELAY - 4.0e-6 - pwD - POWER_DELAY
- PRG_START_DELAY - DELAY_BLANK
- POWER_DELAY - 4.0e-6);
decpower(pwClvl);
decrgpulse(pwC,zero,4.0e-6,0.0);
/* Turn off D decoupling */
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3blank();
dec3phase(three);
dec3power(pwDlvl);
dec3rgpulse(pwD,three,4.0e-6,0.0);
/* Turn off D decoupling */
delay(0.2e-6);
zgradpulse(gzlvl9,gt9);
delay(200.0e-6);
if (shared_CT[A] == 'n')
{
dec2rgpulse(pwN,t5,2.0e-6,0.0);
decpower(d_ca180);
dec2phase(t6);
delay(bigTN - tau2 - POWER_DELAY);
dec2rgpulse(2*pwN,t6,0.0,0.0);
decshaped_pulse(shca180,pwca180,zero,0.0,0.0);
dec2phase(t7);
delay(bigTN - WFG_START_DELAY - pwca180 - WFG_STOP_DELAY
- gt1 - 2.0*GRADIENT_DELAY - 500.2e-6
- POWER_DELAY - 4.0e-6 - WFG_START_DELAY
- pwco180 - WFG_STOP_DELAY);
delay(0.2e-6);
zgradpulse(gzlvl1,gt1);
delay(500.0e-6);
decpower(d_co180);
decshaped_pulse(shco180,pwco180,zero,4.0e-6,0.0);
delay(tau2);
sim3pulse(pw,0.0,pwN,zero,zero,t7,0.0,0.0);
}
else if (shared_CT[A] == 'y')
{
dec2rgpulse(pwN,t5,2.0e-6,0.0);
decpower(d_co180);
dec2phase(t6);
if (bigTN - tau2 >= 0.2e-6)
{
delay(tau2);
decshaped_pulse(shco180,pwco180,zero,4.0e-6,0.0);
decpower(d_ca180);
delay(0.2e-6);
zgradpulse(gzlvl1,gt1);
delay(500.0e-6);
delay(bigTN - 4.0e-6 - WFG_START_DELAY - pwco180 - WFG_STOP_DELAY
- POWER_DELAY - gt1 - 500.2e-6 - 2.0*GRADIENT_DELAY
- WFG_START_DELAY - pwca180 - WFG_STOP_DELAY);
decshaped_pulse(shca180,pwca180,zero,0.0,0.0);
dec2rgpulse(2*pwN,t6,0.0,0.0);
delay(bigTN - tau2);
}
else
{
delay(tau2);
decshaped_pulse(shco180,pwco180,zero,4.0e-6,0.0);
delay(0.2e-6);
zgradpulse(gzlvl1,gt1);
delay(500.0e-6);
decpower(d_ca180);
delay(bigTN - 4.0e-6 - WFG_START_DELAY - pwco180
- WFG_STOP_DELAY - gt1 - 500.2e-6 - 2.0*GRADIENT_DELAY
- POWER_DELAY - WFG_START_DELAY - pwca180 - WFG_STOP_DELAY);
decshaped_pulse(shca180,pwca180,zero,0.0,0.0);
delay(tau2 - bigTN);
dec2rgpulse(2.0*pwN,t6,0.0,0.0);
}
sim3pulse(pw,0.0,pwN,zero,zero,t7,0.0,0.0);
}
/* end of shared_CT */
if (nietl_flg[A] == 'n')
{
decpower(pwClvl);
decrgpulse(pwC,zero,4.0e-6,0.0);
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(2.0e-6);
dec2phase(zero);
delay(tauf - POWER_DELAY - 4.0e-6
- pwC - gt6 - 2.2e-6);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
txphase(one);
dec2phase(one);
delay(tauf - gt6 - 200.2e-6);
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(200.0e-6);
sim3pulse(pw,0.0,pwN,one,zero,one,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
txphase(zero);
dec2phase(zero);
delay(tauf - gt7 - 2.2e-6);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
delay(tauf - gt7 - 200.2e-6);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(200.0e-6);
sim3pulse(pw,0.0e-6,pwN,zero,zero,zero,0.0,0.0);
}
else
{ /* nietl_flg == y */
/* shaped pulse */
obspower(tpwrsl1);
shaped_pulse(shp_sl,pw_sl1,zero,2.0e-6,0.0);
delay(2.0e-6);
obspower(tpwr);
/* shaped pulse */
decpower(pwClvl);
decrgpulse(pwC,zero,4.0e-6,0.0);
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(2.0e-6);
dec2phase(zero);
delay(tauf
- POWER_DELAY - 2.0e-6 - WFG_START_DELAY
- pw_sl1 - WFG_STOP_DELAY - 2.0e-6 - POWER_DELAY
- POWER_DELAY - 4.0e-6
- pwC - gt6 - 2.2e-6);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
txphase(one);
dec2phase(zero);
delay(tauf - gt6 - 200.2e-6);
delay(0.2e-6);
zgradpulse(gzlvl6,gt6);
delay(200.0e-6);
sim3pulse(pw,0.0,pwN,one,zero,zero,0.0,0.0);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(2.0e-6);
txphase(zero);
dec2phase(zero);
delay(tauf - gt7 - 2.2e-6);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
txphase(one);
dec2phase(one);
delay(tauf - gt7 - 200.2e-6);
delay(0.2e-6);
zgradpulse(gzlvl7,gt7);
delay(200.0e-6);
sim3pulse(pw,0.0e-6,pwN,one,zero,one,0.0,0.0);
txphase(zero);
} /* end of nietl_flg == y */
delay(gt2 +gstab -0.5*(pwN-pw) -2.0*pw/PI);
rgpulse(2*pw,zero,0.0,0.0);
delay(2.0e-6);
zgradpulse(icosel*gzlvl2,gt2);
decpower(dpwr); /* NO 13C decoupling */
dec2power(dpwr2); /* NO 15N decoupling */
delay(gstab -2.0e-6 -2.0*GRADIENT_DELAY -2.0*POWER_DELAY);
lk_sample();
/* BEGIN ACQUISITION */
status(C);
setreceiver(t8);
}
pulsesequence ()
{
double gstab = getval("gstab"),
gt1 = getval("gt1"),
gzlvl1 = getval("gzlvl1"),
gt2 = getval("gt2"),
gzlvl2 = getval("gzlvl2"),
mix = getval("mix"),
wrefpw = getval("wrefpw"),
wrefpwr = getval("wrefpwr"),
wrefpwrf = getval("wrefpwrf"),
phincr1 = getval("phincr1"),
flippwr = getval("flippwr"),
flippwrf = getval("flippwrf"),
flippw = getval("flippw"),
trimpwr = getval("trimpwr"),
gt0 = getval("gt0"),
gzlvl0 = getval("gzlvl0"),
trim = getval("trim"),
h1freq_local = getval("h1freq_local"),
gcal_local = getval("gcal_local"),
coil_size = getval("coil_size"),
zqpw=getval("zqpw"),
zqpwr=getval("zqpwr"),
swfactor = 9.0, /* do the adiabatic sweep over 9.0*sw */
gzlvlzq,invsw;
int iphase = (int) (getval("phase") + 0.5);
char sspul[MAXSTR], trim_flg[MAXSTR], wrefshape[MAXSTR],flipback[MAXSTR],
zqshape[MAXSTR],
zqflg[MAXSTR], alt_grd[MAXSTR],flipshape[MAXSTR];
getstr("sspul", sspul);
getstr("trim_flg", trim_flg);
getstr("wrefshape", wrefshape);
getstr("flipshape", flipshape);
getstr("flipback", flipback);
getstr("zqshape", zqshape);
getstr("zqflg", zqflg);
getstr("alt_grd",alt_grd);
rof1 = getval("rof1"); if(rof1 > 2.0e-6) rof1=2.0e-6;
if (phincr1 < 0.0) phincr1=360+phincr1;
initval(phincr1,v13);
if (coil_size == 0) coil_size=16;
invsw = sw*swfactor;
if (invsw > 60000.0) invsw = 60000.0; /* do not exceed 60 kHz */
invsw = invsw/0.97; /* correct for end effects of the cawurst-20 shape */
if ((zqflg[0] == 'y') && (mix < 0.051))
{
printf("Mixing time should be more than 51 ms for zero quantum suppression\n");
psg_abort(1);
}
gzlvlzq=(invsw*h1freq_local*2349)/(gcal_local*coil_size*sfrq*1e+6);
settable(t1,16,phi1);
settable(t2,16,phi2);
settable(t3,16,phi3);
settable(t4,16,phi4);
settable(t5,16,phi5);
settable(t7,16,phi7);
settable(t8,16,phi8);
settable(t6,16,rec);
settable(t9,16,phi9);
sub(ct,ssctr,v12);
getelem(t1,v12,v1);
getelem(t2,v12,v2);
getelem(t3,v12,v3);
getelem(t4,v12,v4);
getelem(t5,v12,v5);
getelem(t6,v12,oph);
getelem(t7,v12,v7);
getelem(t8,v12,v8);
getelem(t9,v12,v6);
if (zqflg[0] == 'y') add(oph,two,oph);
mod2(ct,v10); /*changing gradient sign on even transitents */
if (iphase == 2)
{ incr(v1); incr(v6); }
/* HYPERCOMPLEX MODE USES REDFIELD TRICK TO MOVE AXIAL PEAKS TO EDGE */
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v9);
if ((iphase == 1)||(iphase == 2)) {add(v1,v9,v1); add(oph,v9,oph), add(v6,v9,v6);}
status(A);
obspower(tpwr); obspwrf(4095.0); decpower(dpwr);
if (sspul[A] == 'y')
{
zgradpulse(gzlvl0,gt0);
rgpulse(pw,zero,rof1,rof1);
zgradpulse(gzlvl0,gt0);
}
delay(d1);
status(B);
obsstepsize(45.0);
initval(7.0,v11);
xmtrphase(v11);
rgpulse(pw,v1,rof1,rof1);
if (trim_flg[0] == 'y')
{ obspower(trimpwr);
rgpulse(trim,v6,rof1,rof1);
obspower(tpwr);
}
xmtrphase(zero);
if (trim_flg[0] == 'y')
{
if (d2-2.0*pw/3.14 - 2.0*rof1 - SAPS_DELAY - POWER_DELAY> 0)
delay(d2-2.0*pw/3.14-2.0*rof1-SAPS_DELAY - POWER_DELAY);
else
delay(0.0);
}
else
{
if (d2-4.0*pw/3.14 - 2.0*rof1 - SAPS_DELAY> 0)
delay(d2-4.0*pw/3.14-2.0*rof1-SAPS_DELAY);
else
delay(0.0);
}
rgpulse(pw,v7,rof1,rof1);
if (zqflg[0] == 'y')
{
ifzero(v10); rgradient('z',gzlvlzq);
elsenz(v10); rgradient('z',-1.0*gzlvlzq); endif(v10);
obspower(zqpwr);
shaped_pulse(zqshape,zqpw,zero,rof1,rof1);
obspower(tpwr);
rgradient('z',0.0);
delay((mix-0.050-gt1)*0.7);
if (alt_grd[0] == 'y')
{
ifzero(v10); zgradpulse(gzlvl1,gt1);
elsenz(v10); zgradpulse(-1.0*gzlvl1,gt1); endif(v10);
}
else zgradpulse(gzlvl1,gt1);
if (flipback[0] == 'n')
delay((mix-0.05-gt1)*0.3);
else
{ delay((mix-0.05-gt1)*0.3 - flippw - rof1);
obsstepsize(1.0);
xmtrphase(v13);
add(v8,two,v8);
obspower(flippwr+6); obspwrf(flippwrf);
shaped_pulse(flipshape,flippw,v8,rof1,rof1);
xmtrphase(zero);
add(v8,two,v8);
obspower(tpwr); obspwrf(4095.0);
}
}
else
{
delay(mix*0.7);
if (alt_grd[0] == 'y')
{
ifzero(v10); zgradpulse(gzlvl1,gt1);
elsenz(v10); zgradpulse(-1.0*gzlvl1,gt1); endif(v10);
}
else zgradpulse(gzlvl1,gt1);
if (flipback[0] == 'n')
delay(mix*0.3-gt2);
else
{ delay(mix*0.3 - flippw - rof1);
obsstepsize(1.0);
xmtrphase(v13);
add(v8,two,v8);
obspower(flippwr+6); obspwrf(flippwrf);
shaped_pulse(flipshape,flippw,v8,rof1,rof1);
xmtrphase(zero);
add(v8,two,v8);
obspower(tpwr); obspwrf(4095.0);
}
}
obspower(tpwr);
rgpulse(pw,v8,rof1,rof1);
if (alt_grd[0] == 'y')
{
ifzero(v10); zgradpulse(gzlvl2,gt2);
elsenz(v10); zgradpulse(-1.0*gzlvl2,gt2); endif(v10);
}
else zgradpulse(gzlvl2,gt2);
delay(gstab);
obspower(wrefpwr+6); obspwrf(wrefpwrf);
shaped_pulse(wrefshape,wrefpw,v5,rof1,rof1);
obspower(tpwr); obspwrf(4095.0);
rgpulse(2.0*pw,v4,rof1,rof1);
if (alt_grd[0] == 'y')
{
ifzero(v10); zgradpulse(gzlvl2,gt2);
elsenz(v10); zgradpulse(-1.0*gzlvl2,gt2); endif(v10);
}
else zgradpulse(gzlvl2,gt2);
delay(gstab);
if (alt_grd[0] == 'y')
{
ifzero(v10); zgradpulse(1.2*gzlvl2,gt2);
elsenz(v10); zgradpulse(-1.2*gzlvl2,gt2); endif(v10);
}
else zgradpulse(1.2*gzlvl2,gt2);
delay(gstab);
obspower(wrefpwr+6); obspwrf(wrefpwrf);
shaped_pulse(wrefshape,wrefpw,v3,rof1,rof1);
obspower(tpwr); obspwrf(4095.0);
rgpulse(2.0*pw,v2,rof1,rof2);
if (alt_grd[0] == 'y')
{
ifzero(v10); zgradpulse(1.2*gzlvl2,gt2);
elsenz(v10); zgradpulse(-1.2*gzlvl2,gt2); endif(v10);
}
else zgradpulse(1.2*gzlvl2,gt2);
delay(gstab);
status(C);
}
pulsesequence()
{
/* DECLARE AND LOAD VARIABLES */
char f1180[MAXSTR],
bottom[MAXSTR],
right[MAXSTR],
C13refoc[MAXSTR];
int t1_counter;
double tau1, /* t1 delay */
taua=getval("taua"), /* < 1 / 4J(NH) 2.25 ms */
taub=getval("taub"), /* 1 / 4J(NH) 2.75 ms */
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
compH = getval("compH"), /* adjustment for H1 amplifier compression */
compN = getval("compN"), /* adjustment for N15 amplifier compression */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
pwHs = getval("pwHs"), /* H1 90 degree pulse length at tpwrs */
tpwrs, /* power for the pwHs ("H2Osinc") pulse */
finepwrf = getval("finepwrf"), /* fine power adjustment */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
sw1 = getval("sw1"),
gt3 = getval("gt3"),
gt4 = getval("gt4"),
gt5 = getval("gt5"),
gzlvlr = getval("gzlvlr"),
gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5");
getstr("f1180",f1180);
getstr("bottom",bottom);
getstr("right",right);
getstr("C13refoc",C13refoc);
/* INITIALIZE VARIABLES */
compN=compN;
compC=compC;
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /*needs 1.69 times more*/
tpwrs = (int) (tpwrs); /*power than a square pulse */
tpwrs = tpwrs+6; /* increase attenuator setting so that
fine power control can be varied about the nominal 2048 value */
/* check validity of parameter range */
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y'))
{
printf("incorrect Dec1 decoupler flags! ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y'))
{
printf("incorrect Dec2 decoupler flags! ");
psg_abort(1);
}
if( dpwr > 50 )
{
printf("don't fry the probe, dpwr too large! ");
psg_abort(1);
}
if( dpwr2 > 50 )
{
printf("don't fry the probe, dpwr2 too large! ");
psg_abort(1);
}
if( pwNlvl > 63 )
{
printf("don't fry the probe, pwNlvl too large !");
psg_abort(1);
}
if( pwClvl > 63 )
{
printf("don't fry the probe, pwClvl too large !");
psg_abort(1);
}
if( gzlvlr > 500 )
{
printf("don't fry the probe, gzlvl0 too large !");
psg_abort(1);
}
/* LOAD VARIABLES */
settable(t1, 8, phi1);
if (bottom[A]=='y')
{
settable(t2, 1, ph_y);
settable(t5, 2, phi5);
settable(t7, 2, phi6);
}
else
{
settable(t2, 1, phy);
settable(t5, 2, phi6);
settable(t7, 2, phi5);
}
if (right[A]=='y')
settable(t3, 1, ph_y);
else
settable(t3, 1, phy);
settable(t4, 8, phi4);
settable(t6, 8, rec);
/* Set up f1180 */
tau1 = d2;
if(f1180[A] == 'y' && C13refoc[A] == 'n')
tau1 += ( 1.0 / (2.0*sw1) - pw);
if(f1180[A] == 'y' && C13refoc[A] == 'y')
tau1 += ( 1.0 / (2.0*sw1) - 2.0*pwC - 4.0e-6 - pw);
if(f1180[A] == 'n' && C13refoc[A] == 'n')
tau1 = tau1 - pw;
if(f1180[A] == 'n' && C13refoc[A] == 'y')
tau1 = ( tau1 - 2.0*pwC - 4.0e-6 - pw);
if(tau1 < 0.2e-6) tau1 = 0.2e-6;
/* set up for complex FID */
if (phase1 == 2)
{
tsadd(t2,2,4);
tsadd(t3,2,4);
tsadd(t6,2,4);
}
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1 ) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if( t1_counter %2 ) {
tsadd(t1,2,4);
tsadd(t4,2,4);
tsadd(t6,2,4);
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obsoffset(tof);
decoffset(dof+(50*dfrq)); /* 160 ppm for DEC */
dec2offset(dof2-(45*dfrq2)); /* Middle of imino-N15 = 155 ppm */
obspower(tpwr);
obspwrf(4095.0);
decpower(pwClvl);
dec2power(pwNlvl);
txphase(two);
decphase(zero);
dec2phase(zero);
delay(d1);
rcvroff();
delay(20.0e-6);
rgpulse(pw,two,0.0,0.0);
txphase(zero);
zgradpulse(gzlvl5, gt5);
delay(taua - gt5 - 2.0*GRADIENT_DELAY); /* delay < 1/4J(XH) */
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
txphase(one);
zgradpulse(gzlvl5, gt5);
delay(taua - gt5 - 2.0*GRADIENT_DELAY);
if (phase1 == 1)
sim3pulse(pw,0.0,pwN,one,zero,t1,0.0,0.0);
if (phase1 == 2)
sim3pulse(pw,0.0,pwN,one,zero,t4,0.0,0.0);
txphase(zero);
decphase(zero);
if (C13refoc[A] == 'y')
{
if (tau1>0.001)
{
zgradpulse(gzlvlr,(0.8*tau1/2.0 - 2.0*GRADIENT_DELAY ));
delay(0.2*tau1/2.0 );
}
else
delay(tau1/2.0);
decrgpulse(pwC,zero,0.0,0.0);
decrgpulse(2.0*pwC,one,2.0e-6,0.0);
decrgpulse(pwC,zero,2.0e-6,0.0);
if (tau1>0.001)
{
zgradpulse(-gzlvlr,(0.8*tau1/2.0 - 2.0*GRADIENT_DELAY ));
delay(0.2*tau1/2.0 );
}
else
if ((tau1/2.0)>0.0) delay(tau1/2.0);
}
else
{
if (tau1>0.001)
{
zgradpulse(gzlvlr,(0.8*tau1 - 2.0*GRADIENT_DELAY));
delay(0.2*tau1);
zgradpulse(-gzlvlr,(0.8*tau1 - 2.0*GRADIENT_DELAY ));
delay(0.2*tau1);
}
else
delay(tau1);
}
rgpulse(pw,t2,0.0,0.0);
txphase(zero);
dec2phase(zero);
zgradpulse(gzlvl3,gt3);
delay(taub - gt3 - 2.0*GRADIENT_DELAY);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,0.0,0.0);
dec2phase(t3);
zgradpulse(gzlvl3,gt3);
delay(taub - gt3 - 2.0*GRADIENT_DELAY);
sim3pulse(pw,0.0,pwN,zero,zero,t3,0.0,0.0);
txphase(t5);
dec2phase(zero);
obspower(tpwrs);
obspwrf(finepwrf);
zgradpulse(gzlvl4,gt4);
delay(taub - gt4 - 4.0*POWER_DELAY - rof1 - 2.0*GRADIENT_DELAY - pwHs);
shaped_pulse("H2Osinc",pwHs,t5,0.0,0.0);
txphase(t7);
obspower(tpwr);
obspwrf(4095.0);
sim3pulse(2.0*pw,0.0e-6,2.0*pwN,t7,zero,zero,rof1,rof1);
txphase(t5);
obspwrf(finepwrf);
obspower(tpwrs);
shaped_pulse("H2Osinc",pwHs,t5,0.0,0.0);
txphase(zero);
obspower(tpwr);
obspwrf(4095.0);
zgradpulse(gzlvl4,gt4);
delay(taub - gt4 - 6.0*POWER_DELAY - rof1 - 2.0*GRADIENT_DELAY
- 0.5*(pwN-pw) - pwHs);
dec2rgpulse(pwN,zero,0.0,0.0);
decpower(dpwr);
dec2power(dpwr2);
/* acquire data */
status(C);
setreceiver(t6);
}
pulsesequence()
{
/* DECLARE VARIABLES */
char
satmode[MAXSTR],
f1180[MAXSTR],
abfilter[MAXSTR]; /* set 'a' for inphase and 'b' for antiphase */
int t1_counter,icosel,first_FID;
double /* DELAYS */
tau1, /* t1/2 */
tauhn,taunco,taucoca,taunca,tauhaca,taucaha,taucacb,
/* COUPLINGS */
jhn = getval("jhn"),
jnco = getval("jnco"),
jcoca = getval("jcoca"),
jnca = getval("jnca"),
jhaca = getval("jhaca"),
jcaha = getval("jcaha"),
jcacb = getval("jcacb"),
/* PULSES */
pwN = getval("pwN"), /* PW90 for N-nuc */
pwC = getval("pwC"), /* PW90 for C-nuc */
pwHs = getval("pwHs"),
/* POWER LEVELS */
satpwr = getval("satpwr"), /* low power level for presat */
tpwrsf_d = getval("tpwrsf_d"), /* fine power level for first pwHs */
tpwrsf_u = getval("tpwrsf_u"), /* fine power level for second pwHs */
tpwrs,
pwClvl = getval("pwClvl"), /* power level for C hard pulses */
compC = getval("compC"), /* amplifier compression factor */
compH = getval("compH"), /* amplifier compression factor */
pwNlvl = getval("pwNlvl"), /* power level for N hard pulses */
rf90onco,pw90onco, /* power level/pw for CO 90 pulses */
rf180onco,pw180onco, /* power level/pw for CO 180 pulses */
rf180offca,pw180offca, /* power level/pw for CA 180 pulses */
/* CONSTANTS */
kappa,
lambda = getval("lambda"), /* scaling factor for JNCa */
/* GRADIENT DELAYS AND LEVELS */
gt0 = getval("gt0"), /* gradient time */
gt1 = getval("gt1"), /* gradient time */
gt3 = getval("gt3"), /* gradient time */
gt5 = getval("gt5"),
gstab = getval("gstab"),
gzlvl0 = getval("gzlvl0"), /* level of gradient */
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gzlvl3 = getval("gzlvl3"),
gzlvl5 = getval("gzlvl5");
/* LOAD VARIABLES */
getstr("satmode",satmode);
getstr("f1180",f1180);
getstr("abfilter",abfilter);
/* check validity of parameter range */
if ((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y' ))
{
printf("incorrect Dec1 decoupler flags! ");
psg_abort(1);
}
if ((dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y' ))
{
printf("incorrect Dec2 decoupler flags! ");
psg_abort(1);
}
if ( satpwr > 8 )
{
printf("satpwr too large !!! ");
psg_abort(1);
}
if ( dpwr > 50 )
{
printf("don't fry the probe, dpwr too large! ");
psg_abort(1);
}
if ( dpwr2 > 50 )
{
printf("don't fry the probe, dpwr2 too large! ");
psg_abort(1);
}
/* LOAD VARIABLES */
settable(t1, 1, phi1);
settable(t2, 1, phi2);
settable(t3, 1, phi3);
settable(t4, 2, phi4);
settable(t6, 4, phi6);
settable(t7, 2, phi7);
/* INITIALIZE VARIABLES AND POWER LEVELS FOR PULSES */
tauhn = ((jhn != 0.0) ? 1/(4*(jhn)) : 2.25e-3);
taunco = ((jnco !=0.0) ? 1/(4*(jnco)) : 16.6e-3);
taucoca = ((jcoca !=0.0) ? 1/(4*(jcoca)) : 4.5e-3);
taunca = ((jnca !=0.0) ? 1/(4*(jnca)) : 12e-3);
tauhaca = ((jhaca !=0.0) ? 1/(4*(jhaca)) : 12e-3);
taucaha = ((jcaha !=0.0) ? 1/(4*(jcaha)) : 12e-3);
taucacb = ((jcacb !=0.0) ? 1/(4*(jcacb)) : 12e-3);
if((getval("arraydim") < 1.5) || (ix==1))
first_FID = 1;
else
first_FID = 0;
/* 90 degree pulse on CO, null at Ca 118ppm away */
pw90onco = sqrt(15.0)/(4.0*118.0*dfrq);
rf90onco = (4095.0*pwC*compC)/pw90onco;
rf90onco = (int) (rf90onco + 0.5);
if(rf90onco > 4095.0)
{
if(first_FID)
printf("insufficient power for pw90onco -> rf90onco (%.0f)\n", rf90onco);
rf90onco = 4095.0;
pw90onco = pwC;
}
/* 180 degree pulse on CO, null at Ca 118ppm away */
pw180onco = sqrt(3.0)/(2.0*118.0*dfrq);
rf180onco = (4095.0*pwC*compC*2.0)/pw180onco;
rf180onco = (int) (rf180onco + 0.5);
if(rf180onco > 4095.0)
{
if(first_FID)
printf("insufficient power for pw180onco -> rf180onco (%.0f)\n", rf180onco);
rf180onco = 4095.0;
pw180onco = pwC*2.0;
}
pw180offca = pw180onco; rf180offca = rf180onco;
/* Phase incrementation for hypercomplex data */
kappa=(taunco - tauhn - gt1 - gstab)/(0.5*ni/sw1)-0.001;
if (kappa > 1.0)
{
kappa=1.0-0.01;
}
if ( phase1 == 1) /* Hypercomplex in t2 */
{
icosel = -1;
tsadd(t2, 2, 4);
tsadd(t3, 2, 4);
}
else icosel = 1;
if (ix == 1)
printf("semi constant-time factor %4.6f\n",kappa);
/* calculate modification to phases based on current t1 values
to achieve States-TPPI acquisition */
if (ix == 1)
d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5);
if (t1_counter %2) /* STATES-TPPI */
{
tsadd(t1,2,4);
tsadd(t7,2,4);
}
/* set up so that get (-90,180) phase corrects in F1 if f1180 flag is y */
tau1 = d2;
if (f1180[A] == 'y') tau1 += ( 1.0/(2.0*sw1));
tau1 = tau1/2.0;
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69)); /*needs 1.69 times more*/
tpwrs = (int) (tpwrs); /*power than a square pulse */
if (tpwrsf_d<4095.0) tpwrs=tpwrs+6.0; /* allow for fine power control via tpwrsf_d */
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(satpwr); /* Set power for presaturation */
decpower(pwClvl); /* Set decoupler1 power to pwClvl */
decpwrf(rf90onco);
dec2power(pwNlvl); /* Set decoupler2 power to pwNlvl */
/* Presaturation Period */
if (satmode[0] == 'y')
{
rgpulse(d1,zero,rof1,rof1);
obspower(tpwr); /* Set power for hard pulses */
}
else
{
obspower(tpwr); /* Set power for hard pulses */
delay(d1);
}
status(B);
rcvroff();
/* eliminate all magnetization originating on 13C */
decpwrf(rf90onco);
decrgpulse(pw90onco,zero,0.0,0.0);
zgradpulse(gzlvl0,gt0);
delay(gstab);
/* shaped pulse for water flip-back */
obspower(tpwrs); obspwrf(tpwrsf_d);
shaped_pulse("H2Osinc_d",pwHs,one,rof1,0.0);
delay(2.0e-6);
obspower(tpwr); obspwrf(4095.0);
/* shaped pulse */
/* transfer from HN to N by INEPT */
rgpulse(pw,zero,rof1,0.0); /* 90 for 1H */
zgradpulse(gzlvl0*1.3,gt0);
delay(gstab);
delay(tauhn - gt0 - gstab); /* 1/4JHN */
sim3pulse(2.0*pw,0.0,2.0*pwN,zero,zero,zero,0.0,0.0); /* 180 for 1H and 15N */
delay(tauhn - gt0 - gstab); /* 1/4JHN */
zgradpulse(gzlvl0*1.3,gt0);
delay(gstab);
rgpulse(pw,three,rof1,0.0);
zgradpulse(gzlvl3,gt3);
delay(gstab);
/* start transfer from N to CO */
dec2rgpulse(pwN,zero,0.0,0.0);
delay(taunco - gt0 - gstab - POWER_DELAY - 0.5*pw180onco); /* 1/4J(NCO) */
decpwrf(rf180onco); /* Set decoupler1 power to rf180onco */
zgradpulse(gzlvl0,gt0);
delay(gstab);
sim3pulse(0.0,pw180onco,2.0*pwN,zero,zero,zero,rof1,rof1); /* 180 for 13C' and 15N */
zgradpulse(gzlvl0,gt0);
delay(gstab);
decpwrf(rf90onco); /* Set decoupler1 power to rf90onco */
delay(taunco - gt0 - gstab - POWER_DELAY - 0.5*pw180onco); /* 1/4J(NCO) */
dec2rgpulse(pwN,one,0.0,0.0); /* 90 for 15N */
zgradpulse(gzlvl3*1.1,gt3);
delay(gstab);
decphase(t4);
decrgpulse(pw90onco,t4,0.0,0.0); /* 90 for 13C' */
if (abfilter[0] == 'b')
{
decpwrf(rf180offca); /* Set decoupler1 power to rf180offca */
decshaped_pulse("offC3",pw180offca,zero,0.0,0.0);
decpwrf(rf180onco); /* Set decoupler1 power to rf180onco */
delay(taucoca - gt0 - gstab - 0.5*pw180onco - pw180offca - 2.0*POWER_DELAY); /* 1/4J(COCA) */
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
decrgpulse(pw180onco,zero,0.0,0.0); /* 180 13C' */
decpwrf(rf180offca); /* Set decoupler1 power to rf180offca */
decshaped_pulse("offC3",pw180offca,zero,0.0,0.0);
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
delay(taucoca - gt0 - gstab - 0.5*pw180onco - pw180offca - 2.0*POWER_DELAY); /* 1/4J(NCO) */
decpwrf(rf90onco); /* Set decoupler1 power to rf90onco */
decrgpulse(pw90onco,one,0.0,0.0); /* 90 for 13C' do not touch alpha's */
/* Field crusher gradient */
zgradpulse(gzlvl3*0.9,gt3);
delay(gstab);
/* Field crusher gradient */
}
if (abfilter[0] == 'a')
{
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
decpwrf(rf180offca); /* Set decoupler1 power to rf180offca */
delay(0.5*taucoca - gt0 - gstab - POWER_DELAY - 0.5*pw180offca); /* 1/8J(COCA) */
decshaped_pulse("offC3",pw180offca,zero,0.0,0.0);
delay(0.5*taucoca - gt0 - gstab - POWER_DELAY - 0.5*pw180offca - 0.5*pw180onco); /* 1/8J(COCA) */
decpwrf(rf180onco); /* Set decoupler1 power to rf180onco */
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
decrgpulse(pw180onco,zero,0.0,0.0); /* 180 13C' */
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
decpwrf(rf180offca);
delay(0.5*taucoca - gt0 - gstab - POWER_DELAY - 0.5*pw180onco - 0.5*pw180offca); /* 1/8J(COCA) */
decshaped_pulse("offC3",pw180offca,zero,0.0,0.0);
delay(0.5*taucoca - gt0 - gstab - POWER_DELAY - 0.5*pw180offca); /* 1/8J(COCA) */
decpwrf(rf90onco); /* Set decoupler1 power to rf90onco */
zgradpulse(gzlvl0*0.9,gt0);
delay(gstab);
decrgpulse(pw90onco,zero,0.0,0.0); /* 90 for 13C' */
/* Field crusher gradient */
zgradpulse(gzlvl3*0.9,gt3);
delay(gstab);
/* Field crusher gradient */
}
dec2rgpulse(pwN,t1,0.0,0.0); /* 90 15N */
if (lambda>0.0)
{
delay(lambda*tau1); /* (lambda)t1/2 */
decpwrf(rf180offca);
sim3shaped_pulse("","offC3","",0.0,pw180offca,2.0*pwN,zero,zero,zero,rof1,rof1);
delay(lambda*tau1); /* (lambda)t1/2 */
}
delay(tau1); /* t1/2 */
delay(taunco - tauhn - pw180onco);
decpwrf(rf180onco);
decrgpulse(pw180onco,zero,0.0,0.0); /* 180 for 13C' */
delay((1-kappa)*tau1); /* (1-k)t1/2 */
dec2rgpulse(2.0*pwN,zero,0.0,0.0); /* 180 for 15N */
delay((taunco - tauhn - gt1 - gstab) - kappa*tau1); /* 1/4J(NCO) - 1/4J(NH) - kt1/2 */
zgradpulse(gzlvl1,gt1);
delay(gstab);
/* start TROSY transfer from N to HN */
sim3pulse(pw,0.0,0.0,t2,zero,zero,rof1,rof1);
zgradpulse(gzlvl5,gt5);
delay(gstab);
delay(tauhn - gt5 - gstab - POWER_DELAY);
decpwrf(rf180onco);
sim3pulse(2.0*pw,pw180onco,2*pwN,zero,zero,zero,rof1,rof1);
delay(tauhn - gt5 - gstab - POWER_DELAY);
zgradpulse(gzlvl5,gt5);
delay(gstab);
decpwrf(rf90onco);
sim3pulse(pw,pw90onco,pwN,one,t6,zero,rof1,rof1);
/* shaped pulse WATERGATE */
obspower(tpwrs); obspwrf(tpwrsf_u);
shaped_pulse("H2Osinc_u",pwHs,t2,rof1,0.0);
obspower(tpwr); obspwrf(4095.0);
/* shaped pulse */
zgradpulse(gzlvl5*0.8,gt5);
delay(gstab);
delay(tauhn - gt5 - gstab - 2.0*POWER_DELAY - pwHs);
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,rof1,rof1);
delay(tauhn - gt5 - gstab - 2.0*POWER_DELAY);
decpower(dpwr);
zgradpulse(gzlvl5*0.8,gt5);
delay(gstab);
dec2rgpulse(pwN,t3,0.0,0.0); /* 90 for 15N */
dec2power(dpwr2);
delay((gt1/10.0) -pwN + gstab - POWER_DELAY);
rgpulse(2.0*pw, zero, rof1, rof1);
zgradpulse(gzlvl2*icosel,gt1/10.0);
delay(gstab);
status(C);
setreceiver(t7);
}
pulsesequence()
{
char codec[MAXSTR], codecseq[MAXSTR];
int icosel, t1_counter;
double d2_init = 0.0,
rf0 = 4095, rf200, pw200,
copwr, codmf, cores, copwrf,
tpwrs,
pwHs = getval("pwHs"),
compH = getval("compH"),
pwClvl = getval("pwClvl"),
pwC = getval("pwC"),
compC = getval("compC"),
pwNlvl = getval("pwNlvl"),
pwN = getval("pwN"),
sw1 = getval("sw1"),
swH = getval("swH"),
swC = getval("swC"),
swN = getval("swN"),
swTilt,
angle_H = getval("angle_H"),
angle_C = getval("angle_C"),
angle_N,
cos_H,
cos_C,
cos_N,
mix = getval("mix"),
tauCH = getval("tauCH"), /* 1/4JCH */
tauNH = getval("tauNH"), /* 1/4JNH */
tau1, tau2, tau3,
tofali =getval("tofali"), /* aliphatic protons offset */
dofcaco =getval("dofcaco"), /* offset for caco decoupling, ~120 ppm */
dof = getval("dof"),
gstab = getval("gstab"),
gt0 = getval("gt0"), gzlvl0 = getval("gzlvl0"),
gt1 = getval("gt1"), gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gt3 = getval("gt3"), gzlvl3 = getval("gzlvl3"),
gt4 = getval("gt4"), gzlvl4 = getval("gzlvl4"),
gt5 = getval("gt5"), gzlvl5 = getval("gzlvl5"),
gt6 = getval("gt6"), gzlvl6 = getval("gzlvl6"),
gt7 = getval("gt7"), gzlvl7 = getval("gzlvl7"),
gt8 = getval("gt8"), gzlvl8 = getval("gzlvl8"),
gt9 = getval("gt9"), gzlvl9 = getval("gzlvl9");
/* LOAD VARIABLES */
copwr = getval("copwr"); copwrf = getval("copwrf");
codmf = getval("codmf"); cores = getval("cores");
getstr("codecseq", codecseq); getstr("codec", codec);
/* Load phase cycling variables */
settable(t1, 4, phi1); settable(t2, 2, phi2); settable(t3, 1, phi3);
settable(t4, 8, phi4); settable(t5, 1, phi5); settable(t14, 2, phi14);
settable(t11, 8, rec);
angle_N=0.0; cos_N=0.0;
/* activate auto-calibration flags */
setautocal();
if (autocal[0] == 'n')
{
/* 180 degree adiabatic C13 pulse from 0 to 200 ppm */
pw200 = getval("pw200");
rf200 = (compC*4095.0*pwC*4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35));
rf200 = (int) (rf200 + 0.5);
if (1.0/(4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35)) < pwC )
{ text_error( " Not enough C13 RF. pwC must be %f usec or less.\n",
(1.0e6/(4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35))) );
psg_abort(1); }
}
else /* if autocal = 'y'(yes), 'q'(quiet), r(read), or 's'(semi) */
{
strcpy(codecseq,"Pdec_154p");
if(FIRST_FID) /* call Pbox */
{
ppm = getval("dfrq");
bw = 200.0*ppm; pws = 0.001; ofs = 0.0; nst = 1000;
/* 1 ms long pulse, nst: number of steps */
stC200 = pbox_makeA("stC200", "sech", bw, pws, ofs, compC*pwC, pwClvl, nst);
bw=20.0*ppm; ofs=154*ppm;
Pdec_154p = pbox_Dsh("Pdec_154p", "WURST2", bw, ofs, compC*pwC, pwClvl);
}
rf200 = stC200.pwrf; pw200 = stC200.pw;
}
/* selective H20 one-lobe sinc pulse */
tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69));
tpwrs = (int)(tpwrs+0.5);
/* check validity of parameter range */
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y'))
{ printf("incorrect Dec1 decoupler flags! "); psg_abort(1); }
if((dm2[A] == 'y' || dm2[B] == 'y'))
{ printf("incorrect Dec2 decoupler flags! "); psg_abort(1); }
if ((dpwr > 48) || (dpwr2 > 48))
{ printf("don't fry the probe, dpwr too high! "); psg_abort(1); }
/* set up angles for PR42 experiments */
/* sw1 is used as symbolic index */
if ( sw1 < 1000 ) { printf ("Please set sw1 to some value larger than 1000.\n"); psg_abort(1); }
if (angle_H < 0 || angle_C < 0 || angle_H > 90 || angle_C > 90 )
{ printf("angles must be set between 0 and 90 degree.\n"); psg_abort(1); }
cos_H = cos (PI*angle_H/180); cos_C = cos (PI*angle_C/180);
if ( (cos_H*cos_H + cos_C*cos_C) > 1.0) { printf ("Impossible angle combinations.\n"); psg_abort(1); }
else { cos_N = sqrt(1 - (cos_H*cos_H + cos_C*cos_C) ); angle_N = acos(cos_N)*180/PI; }
if (ix == 1) d2_init = d2;
t1_counter = (int)((d2-d2_init)*sw1 + 0.5);
if(t1_counter % 2) { tsadd(t3,2,4); tsadd(t11,2,4); }
swTilt = swH*cos_H + swC*cos_C + swN*cos_N;
if (phase1 == 1) {;} /* CC */
else if (phase1 == 2) { tsadd(t1, 1, 4); } /* SC */
else if (phase1 == 3) { tsadd(t2, 1, 4); tsadd(t14,1,4); } /* CS */
else if (phase1 == 4) { tsadd(t1, 1, 4); tsadd(t2,1,4); tsadd(t14,1,4); } /* SS */
if ( phase2 == 1 ) { tsadd(t5,2,4); icosel = 1; }
else icosel = -1;
tau1 = 1.0 * t1_counter * cos_H / swTilt;
tau2 = 1.0 * t1_counter * cos_C / swTilt;
tau3 = 1.0 * t1_counter * cos_N / swTilt;
tau1 = tau1/2.0; tau2 = tau2/2.0; tau3 =tau3/2.0;
if (ix ==1 )
{
printf ("Current Spectral Width:\t\t%5.2f\n", swTilt);
printf ("Angle_H: %5.2f \n", angle_H);
printf ("Angle_C: %5.2f \n", angle_C);
printf ("Angle_N: %5.2f \n", angle_N);
printf ("\n\n\n\n\n");
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
delay(d1);
rcvroff();
obsoffset(tofali); obspower(tpwr); obspwrf(4095.0);
decoffset(dof); decpower(pwClvl); decpwrf(rf0);
dec2offset(dof2); dec2power(pwNlvl); dec2pwrf(4095.0);
if (gt0 > 0.2e-6)
{
decrgpulse(pwC,zero,10.0e-6,0.0);
dec2rgpulse(pwN,zero,2.0e-6,2.0e-6);
zgradpulse(gzlvl0,gt0);
delay(gstab);
}
txphase(t1); decphase(t2); dec2phase(zero);
status(B);
rgpulse(pw,t1,4.0e-6,2.0e-6);
zgradpulse(gzlvl3,gt3);
delay(2.0*tauCH - gt3 - 2.0*GRADIENT_DELAY -4.0e-6);
decrgpulse(pwC,t2,2.0e-6,2.0e-6);
decphase(zero);
/*======= Start of c13 evolution ==========*/
if ( ((tau2 -PRG_START_DELAY - POWER_DELAY -pwN - 2.0*pwC/PI -2.0e-6)> 0)
&& ((tau2 -PRG_STOP_DELAY - POWER_DELAY - pwN - 2.0*pwC/PI -2.0e-6)>0)
&& (codec[A] == 'y') )
{
decpower(copwr); decpwrf(copwrf);
decprgon(codecseq,1/codmf,cores);
decon();
delay(tau2 -PRG_START_DELAY - POWER_DELAY -pwN - 2.0*pwC/PI -2.0e-6);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, t1, zero, zero, 0.0, 0.0);
delay(tau2 -PRG_STOP_DELAY - POWER_DELAY - pwN - 2.0*pwC/PI -2.0e-6);
decoff();
decprgoff();
}
else if ( (tau2 -pwN - 2.0*pwC/PI -2.0e-6) > 0)
{
delay(tau2 -pwN - 2.0*pwC/PI -2.0e-6);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, t1, zero, zero, 0.0, 0.0);
delay(tau2 -pwN - 2.0*pwC/PI -2.0e-6);
}
else
{
delay(2.0*tau2);
decphase(t14);
delay(4.0e-6);
sim3pulse(2.0*pw, 2.0*pwC, 2.0*pwN, t1, t14, zero, 0.0, 0.0);
delay(4.0e-6);
}
decpower(pwClvl);
decpwrf(rf0);
decphase(zero);
/*======= End of c13 evolution ==========*/
decrgpulse(pwC,zero, 2.0e-6,2.0e-6);
txphase(zero);
delay(2.0*tauCH + tau1 - gt3 - 4.0*pwC
- gstab -4.0e-6 - 2.0*GRADIENT_DELAY);
zgradpulse(gzlvl3,gt3);
delay(gstab);
decrgpulse(pwC,zero,0.0, 0.0);
decphase(one);
decrgpulse(2.0*pwC, one, 0.2e-6, 0.0);
decphase(zero);
decrgpulse(pwC, zero, 0.2e-6, 0.0);
delay(tau1);
rgpulse(pw,zero,2.0e-6,0.0);
/* ===========Beginning of NOE transfer ======= */
delay(mix - gt4 - gt5 - pwN -pwC - 2.0*gstab);
obsoffset(tof);
zgradpulse(gzlvl4,gt4);
delay(gstab);
decrgpulse(pwC, zero, 2.0e-6, 2.0e-6);
dec2rgpulse(pwN, zero, 2.0e-6, 2.0e-6);
zgradpulse(gzlvl5,gt5);
delay(gstab);
/* H2O relaxes back to +z */
/* =========== End of NOE transfer ======= */
rgpulse(pw, zero, 2.0e-6, 2.0e-6);
zgradpulse(gzlvl6,gt6);
delay(tauNH - gt6 - 4.0e-6 - 2.0*GRADIENT_DELAY);
sim3pulse(2.0*pw, 0.0, 2*pwN, zero, zero, zero, 2.0e-6, 2.0e-6);
delay(tauNH - gt6 - gstab -4.0e-6 - 2.0*GRADIENT_DELAY);
zgradpulse(gzlvl6,gt6);
txphase(one);
delay(gstab);
rgpulse(pw,one,2.0e-6,2.0e-6);
txphase(two);
obspower(tpwrs);
shaped_pulse("H2Osinc", pwHs, two, 2.0e-6, 2.0e-6);
obspower(tpwr);
zgradpulse(gzlvl7,gt7);
dec2phase(t3);
decoffset(dofcaco); /* offset on 120ppm for CaCO decoupling */
decpwrf(rf200); /* fine power for stC200 */
delay(gstab);
dec2rgpulse(pwN,t3,2.0e-6,2.0e-6);
dec2phase(t4);
delay(tau3);
rgpulse(2.0*pw, zero, 2.0e-6, 2.0e-6);
decshaped_pulse("stC200", 1.0e-3, zero, 2.0e-6, 2.0e-6);
delay(tau3);
delay(gt1 +gstab +8.0e-6 - 1.0e-3 - 2.0*pw);
dec2rgpulse(2.0*pwN, t4, 2.0e-6, 2.0e-6);
dec2phase(t5);
zgradpulse(gzlvl1, gt1);
delay(gstab + WFG_START_DELAY + WFG_STOP_DELAY - 2.0*GRADIENT_DELAY);
sim3pulse(pw, 0.0, pwN, zero, zero, t5, 2.0e-6, 2.0e-6);
dec2phase(zero);
zgradpulse(gzlvl8, gt8);
delay(tauNH - gt8 - 2.0*GRADIENT_DELAY -4.0e-6);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 2.0e-6, 2.0e-6);
delay(tauNH - gt8 - gstab -4.0e-6 - 2.0*GRADIENT_DELAY);
zgradpulse(gzlvl8, gt8);
txphase(one); dec2phase(one);
delay(gstab);
sim3pulse(pw, 0.0, pwN, one, zero, one, 2.0e-6, 2.0e-6);
txphase(zero); dec2phase(zero);
zgradpulse(gzlvl9, gt9);
delay(tauNH - gt9 - 2.0*GRADIENT_DELAY -4.0e-6);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 2.0e-6, 2.0e-6);
delay(tauNH - gt9 - 2.0*GRADIENT_DELAY -gstab -4.0e-6);
zgradpulse(gzlvl9, gt9);
delay(gstab);
rgpulse(pw, zero, 2.0e-6, 2.0e-6);
delay((gt1/10.0) +gstab -2.0e-6 - 0.5*pw + 2.0*GRADIENT_DELAY + POWER_DELAY);
rgpulse(2.0*pw, zero, 2.0e-6, 2.0e-6);
zgradpulse(icosel*gzlvl2, gt1/10.0);
dec2power(dpwr2);
delay(gstab);
status(C);
setreceiver(t11);
}
pulsesequence()
{
double gzlvl1 = getval("gzlvl1"),
gt1 = getval("gt1"),
gstab = getval("gstab"),
del = getval("del"),
tweek = getval("tweek"),
prgtime = getval("prgtime"),
prgpwr = getval("prgpwr"),
satpwr = getval("satpwr"),
satdly = getval("satdly"),
gzlvl2 = getval("gzlvl2"),
gt2 = getval("gt2"),
gzlvlhs = getval("gzlvlhs"),
hsgt = getval("hsgt"),
wrefpwr = getval("wrefpwr"),
wrefpw = getval("wrefpw"),
wrefpwrf = getval("wrefpwrf"),
dosytimecubed,dosyfrq,Ddelta;
char delflag[MAXSTR],lkgate_flg[MAXSTR],alt_grd[MAXSTR],prg_flg[MAXSTR];
char satmode[MAXSTR],sspul[MAXSTR],wrefshape[MAXSTR];
getstr("delflag",delflag);
getstr("lkgate_flg",lkgate_flg);
getstr("alt_grd",alt_grd);
getstr("prg_flg",prg_flg);
getstr("satmode",satmode);
getstr("sspul",sspul);
getstr("wrefshape", wrefshape);
//synchronize gradients to srate for probetype='nano'
// Preserve gradient "area"
gt1 = syncGradTime("gt1","gzlvl1",1.0);
gzlvl1 = syncGradLvl("gt1","gzlvl1",1.0);
gt2 = syncGradTime("gt2","gzlvl2",1.0);
gzlvl2 = syncGradLvl("gt2","gzlvl2",1.0);
if (del<(3*gt1+3.0*gstab+2.0*rof1+pw*2.0))
{ abort_message("DgcsteSL error: del is less than %f, too short!",
(3*gt1+3.0*gstab+2.0*rof1+pw*2.0));
}
/* Safety check for the duration of the purge pulse */
if (prgtime > 4.0e-2)
{ text_error("prgtime has been reset to a maximum of 2 ms");
prgtime = 2.0e-3;
}
dosyfrq=sfrq;
Ddelta=gt1; /* the diffusion-encoding pulse width is gt1 */
dosytimecubed=Ddelta*Ddelta*(del-(Ddelta/3.0));
putCmd("makedosyparams(%e,%e)\n",dosytimecubed,dosyfrq);
/* phase cycling calculation */
if (delflag[0]=='y')
{ hlv(ct,v4);
mod2(v4,v2);
mod2(ct,v1);
dbl(v1,v1);
add(v2,v1,v1);
mod4(v1,v1); /* 0 2 1 3 , 1st 90 */
hlv(v4,v4);
mod2(v4,v3);
dbl(v3,v3); /* (0)4 (2)4 */
hlv(v4,v4);
mod2(v4,v2);
dbl(v2,v2); /* (0)8 (2)8 */
hlv(v4,v4);
mod2(v4,v5);
add(v5,v3,v3);
mod4(v3,v3); /* {(0)4 (2)4}2 {(1)4 (3)4}2, 3rd 90 */
hlv(v4,v4);
assign(v4,v6);
mod2(v4,v4);
add(v4,v2,v2);
mod4(v2,v2); /* {(0)8 (2)8}2 {(1)8 (3)8}2, 2nd 90 */
mod2(ct,v10); /* gradients change sign every increment */
assign(v3,oph);
add(oph,v2,oph);
sub(oph,v1,oph);
mod4(oph,oph);
assign(oph,v5);
add(one,v5,v5);
mod2(ct,v7);
dbl(v7,v7);
hlv(v6,v6);
mod2(v6,v6);
dbl(v6,v6);
add(v7,v5,v5); /* the purge pulse is 90 deg. off the receiver phase */
add(v6,v5,v5); /* it is phase alternated every increment */
mod4(v5,v5); /* and also every 64 increments */
}
else
{
assign(ct,v4);
assign(oph,v4); /*v4 used only for normal s2pul- type sequence */
}
add(v3,two,v9);
if (ni > 1.0)
{ abort_message("DgcsteSL is a 2D, not a 3D dosy sequence: please set ni to 0 or 1");
}
/* equilibrium period */
status(A);
obspower(tpwr);
if (sspul[A] == 'y')
{
zgradpulse(gzlvlhs,hsgt);
rgpulse(pw,zero,rof1,rof1);
zgradpulse(gzlvlhs,hsgt);
}
if (satmode[0] == 'y')
{
if (d1 - satdly > 0)
delay(d1 - satdly);
else delay(0.02);
obspower(satpwr);
txphase(v1);
rgpulse(satdly,zero,rof1,rof1);
obspower(tpwr);
delay(1.0e-5);
}
else
{ delay(d1); }
obspower(tpwr);
status(B);
/* first part of bppdel sequence */
if(delflag[0] == 'y')
{ rgpulse(pw, v1, rof1, 0.0); /* first 90, v1 */
if (lkgate_flg[0] == 'y') lk_hold(); /* turn lock sampling off */
if (alt_grd[0] == 'y')
{ ifzero(v10);
zgradpulse(gzlvl1,gt1);
elsenz(v10);
zgradpulse(-1.0*gzlvl1,gt1);
endif(v10);
}
else
zgradpulse(gzlvl1,gt1);
delay(gstab);
rgpulse(pw, v2, rof1, 0.0); /* second 90, v2 */
if (alt_grd[0] == 'y')
{ ifzero(v10); /* compensating AND CPS gradient */
zgradpulse(-1.0*gzlvl1*(1.0-tweek),gt1);
elsenz(v10);
zgradpulse(gzlvl1*(1.0-tweek),gt1);
endif(v10);
}
else /* compensating AND CPS gradient */
zgradpulse(-1.0*gzlvl1*(1.0-tweek),gt1);
delay(gstab);
if (satmode[B] == 'y')
{ obspower(satpwr);
rgpulse(del-3.0*(gt1+gstab)-2.0*rof1-pw*2.0,zero,rof1,rof1);
obspower(tpwr);
}
else
delay(del-3.0*(gt1+gstab)-2.0*rof1-pw*2.0); /* diffusion delay */
if (alt_grd[0] == 'y')
{
ifzero(v10);
zgradpulse(-1.0*gzlvl1*(1.0+tweek),gt1);
elsenz(v10);
zgradpulse(gzlvl1*(1.0+tweek),gt1);
endif(v10);
}
else
zgradpulse(-1.0*gzlvl1*(1.0+tweek),gt1);
delay(gstab);
rgpulse(pw, v3, rof1, 0.0); /* third 90, v3 */
if (alt_grd[0] == 'y')
{
ifzero(v10);
zgradpulse(gzlvl1,gt1);
elsenz(v10);
zgradpulse(-1.0*gzlvl1,gt1);
endif(v10);
}
else
zgradpulse(gzlvl1,gt1);
delay(gstab);
if (alt_grd[0] == 'y')
{ ifzero(v10); zgradpulse(gzlvl2,gt2);
elsenz(v10); zgradpulse(-gzlvl2,gt2); endif(v10);}
else zgradpulse(gzlvl2,gt2);
obspower(wrefpwr+6); obspwrf(wrefpwrf);
delay(gstab);
shaped_pulse(wrefshape,wrefpw,v9,rof1,0.0);
obspower(tpwr); obspwrf(4095.0);
rgpulse(2.0*pw,v3,rof1,0.0);
if (alt_grd[0] == 'y')
{ ifzero(v10); zgradpulse(gzlvl2,gt2);
elsenz(v10); zgradpulse(-gzlvl2,gt2); endif(v10);}
else zgradpulse(gzlvl2,gt2);
obspower(wrefpwr+6); obspwrf(wrefpwrf);
delay(gstab);
if (alt_grd[0] == 'y')
{ ifzero(v10); zgradpulse(1.2*gzlvl2,gt2);
elsenz(v10); zgradpulse(-1.2*gzlvl2,gt2); endif(v10); }
else zgradpulse(1.2*gzlvl2,gt2);
delay(gstab);
shaped_pulse(wrefshape,wrefpw,v9,rof1,0.0);
obspower(tpwr); obspwrf(4095.0);
rgpulse(2.0*pw,v3,rof1,rof1);
if (alt_grd[0] == 'y')
{ ifzero(v10); zgradpulse(1.2*gzlvl2,gt2);
elsenz(v10); zgradpulse(-1.2*gzlvl2,gt2); endif(v10); }
else zgradpulse(1.2*gzlvl2,gt2);
delay(gstab+2.0*pw/3.14);
if (lkgate_flg[0] == 'y') lk_sample(); /* turn lock sampling on */
/* purge pulse to scramble any magnetisation that
is not in phase with the receiver */
if (prg_flg[0] == 'y')
{ obspower(prgpwr);
rgpulse(prgtime, v5, rof1, 0.0);
}
}
else
rgpulse(pw, v4, rof1, rof2); /* first 90, v1 */
status(C);
}
void pulsesequence()
{
double gzlvlE = getval("gzlvlE"),
gtE = getval("gtE"),
EDratio = getval("EDratio"),
gstab = getval("gstab"),
mult = getval("mult"),
pwx180 = getval("pwx180"),
pwxlvl180 = getval("pwxlvl180"),
pwx180r = getval("pwx180r"),
pwxlvl180r = getval("pwxlvl180r"),
tpwr180 = getval("tpwr180"),
pw180 = getval("pw180"),
hsglvl = getval("hsglvl"),
hsgt = getval("hsgt"),
gzlvl5 = getval("gzlvl5"),
tau,
taug;
int icosel,
phase1 = (int)(getval("phase")+0.5),
prgcycle = (int)(getval("prgcycle")+0.5),
ZZgsign;
char pwx180ad[MAXSTR],
pwx180ref[MAXSTR],
bipflg[MAXSTR],
pw180ad[MAXSTR];
//synchronize gradients to srate for probetype='nano'
// Preserve gradient "area"
gtE = syncGradTime("gtE","gzlvlE",0.5);
gzlvlE = syncGradLvl("gtE","gzlvlE",0.5);
getstr("pwx180ad", pwx180ad);
getstr("pwx180ref", pwx180ref);
getstr("pw180ad", pw180ad);
getstr("bipflg",bipflg);
if (bipflg[0] == 'y')
{
tpwr180=getval("tnbippwr");
pw180=getval("tnbippw");
getstr("tnbipshp",pw180ad);
}
if (bipflg[1] == 'y')
{
pwxlvl180=getval("dnbippwr");
pwx180=getval("dnbippw");
getstr("dnbipshp",pwx180ad);
}
tau = 1 / (4*(getval("j1xh")));
if (mult > 0.5)
taug = 2*tau + getval("tauC");
else
taug = gtE + gstab + 2 * GRADIENT_DELAY;
ZZgsign=-1;
if (mult == 2) ZZgsign=1;
icosel = 1;
assign(ct,v17);
assign(zero,v18);
assign(zero,v19);
if (getflag("prgflg") && (satmode[0] == 'y') && (prgcycle > 1.5))
{
hlv(ct,v17);
mod2(ct,v18); dbl(v18,v18);
if (prgcycle > 2.5)
{
hlv(v17,v17);
hlv(ct,v19); mod2(v19,v19); dbl(v19,v19);
}
}
settable(t1, 4, ph1);
settable(t2, 2, ph2);
settable(t3, 8, ph3);
settable(t4, 16, ph4);
settable(t5, 16, ph5);
getelem(t1, v17, v1);
getelem(t3, v17, v3);
getelem(t4, v17, v4);
getelem(t2, v17, v2);
getelem(t5, v17, oph);
assign(zero,v6);
add(oph,v18,oph);
add(oph,v19,oph);
if ((phase1 == 2) || (phase1 == 5))
icosel = -1;
initval(2.0*(double)(((int)(d2*getval("sw1")+0.5)%2)),v14);
/*
mod2(id2, v14);
dbl(v14,v14);
*/
add(v2, v14, v2);
add(oph, v14, oph);
status(A);
obspower(tpwr);
delay(5.0e-5);
if (getflag("sspul"))
steadystate();
if (satmode[0] == 'y')
{
if ((d1-satdly) > 0.02)
delay(d1-satdly);
else
delay(0.02);
if (getflag("slpsat"))
{
shaped_satpulse("relaxD",satdly,zero);
if (getflag("prgflg"))
shaped_purge(v6,zero,v18,v19);
}
else
{
satpulse(satdly,zero,rof1,rof1);
if (getflag("prgflg"))
purge(v6,zero,v18,v19);
}
}
else
delay(d1);
if (getflag("wet"))
wet4(zero,one);
obspower(tpwr);
decpower(pwxlvl180);
status(B);
if (getflag("nullflg"))
{
rgpulse(0.5 * pw, zero, rof1, rof1);
obspower(tpwr180);
delay(tau);
simshaped_pulse(pw180ad,pwx180ad,pw180,pwx180,zero,zero,rof1,rof1);
delay(tau+POWER_DELAY+rof1);
delay(tau+POWER_DELAY+rof1);
simshaped_pulse(pw180ad,pwx180ad,pw180,pwx180,zero,zero,rof1,rof1);
obspower(tpwr);
delay(tau);
rgpulse(0.5 * pw, zero, rof1, rof1);
zgradpulse(hsglvl, hsgt);
delay(1e-3);
}
if (getflag("cpmgflg"))
{
rgpulse(pw, v6, rof1, 0.0);
cpmg(v6, v15);
}
else
rgpulse(pw, v6, rof1, rof1);
obspower(tpwr180);
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
delay(tau+2.0*POWER_DELAY+2.0*rof1);
simshaped_pulse(pw180ad,pwx180ad,pw180,pwx180,zero,zero,rof1,rof1);
delay(tau);
obspower(tpwr);
rgpulse(pw, v1, rof1, rof1);
zgradpulse(hsglvl, 2 * hsgt);
decpower(pwxlvl);
obspower(tpwr180);
delay(1e-3);
decrgpulse(pwx, v2, rof1, 2.0e-6);
delay(d2 / 2);
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
delay(d2 / 2);
delay(taug - POWER_DELAY);
decpower(pwxlvl180r);
decshaped_pulse(pwx180ref, pwx180r, zero, rof1, rof1);
if(mult < 1.5)
{
obspower(tpwr);
if (mult <0.5)
delay(2*rof1);
else
rgpulse(mult*pw,zero,rof1,rof1);
}
else
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
if(mult < 1.5)
delay(4*pwx/PI + 4.0e-6 + taug - gtE - gstab - 2 * GRADIENT_DELAY + WFG_START_DELAY + pw180 + WFG_STOP_DELAY - mult*pw);
else
delay(4*pwx/PI + 4.0e-6 + POWER_DELAY + taug - gtE - gstab - 2 * GRADIENT_DELAY);
zgradpulse(gzlvlE, gtE);
delay(gstab);
decshaped_pulse(pwx180ref, pwx180r, zero, rof1, rof1);
decpower(pwxlvl);
decrgpulse(pwx, v4, 2.0e-6, rof1);
obspower(tpwr);
decpower(pwxlvl180);
zgradpulse(ZZgsign*0.6 * hsglvl, 1.2 * hsgt);
delay(1e-3);
rgpulse(pw, v3, rof1, rof1);
obspower(tpwr180);
shaped_pulse(pw180ad,pw180,zero,rof1,rof1);
zgradpulse(gzlvl5,gtE/2.0);
delay(tau-gtE/2.0+2.0*POWER_DELAY);
simshaped_pulse(pw180ad,pwx180ad,pw180,pwx180,zero,zero,rof1,rof2);
zgradpulse(gzlvl5+icosel * 2.0*gzlvlE/EDratio, gtE/2.0);
delay(tau-gtE/2.0);
decpower(dpwr);
status(C);
}
pulsesequence()
{
int phase, t1_counter;
char C13refoc[MAXSTR], /* C13 sech/tanh pulse in middle of t1 */
TROSY[MAXSTR],
wtg3919[MAXSTR];
double tauxh, tau1, gt2, gt1,
gztm, mix, pw180, pw135, pw120, pw110, p1lvl,
gzlvl1, cycles,
pwNt = 0.0, /* pulse only active in the TROSY option */
gsign = 1.0,
gzlvl3=getval("gzlvl3"),
gt3=getval("gt3"),
JNH = getval("JNH"),
pwN = getval("pwN"),
pwNlvl = getval("pwNlvl"),
pwHs, tpwrs=0.0, /* H1 90 degree pulse length at tpwrs */
compH = getval("compH"),
sw1 = getval("sw1"),
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rfst = 4095.0, /* fine power for the stCall pulse */
/* temporary Pbox parameters */
bw, pws, ofs, ppm, nst, /* bandwidth, pulsewidth, offset, ppm, # steps */
compC = getval("compC"); /* adjustment for C13 amplifier compr-n */
gztm=getval("gztm");
gt2=getval("gt2");
gt1= getval("gt1");
mix=getval("mix");
phase = (int) (getval("phase") + 0.5);
sw1 = getval("sw1");
pw180 = getval("pw180");
gzlvl1 = getval("gzlvl1");
/* INITIALIZE VARIABLES */
pw135 = pw180 / 180.0 * 135.0 ;
pw120 = pw180 / 180.0 * 120.0 ;
pw110 = pw180 / 180.0 * 110.0 ;
p1lvl = tpwr -20*log10(pw180/(compH*2.0*pw));
p1lvl = (int)(p1lvl + 0.5);
cycles = mix / (730.0/180.0 * pw180) - 8.0;
initval(cycles, v10); /* mixing time cycles */
getstr("C13refoc",C13refoc);
getstr("TROSY",TROSY);
getstr("wtg3919",wtg3919);
tauxh = ((JNH != 0.0) ? 1/(4*(JNH)) : 2.25e-3);
setautocal(); /* activate auto-calibration flags */
if (autocal[0] == 'n')
{
if (C13refoc[A]=='y')
{
/* 180 degree adiabatic C13 pulse from 0 to 200 ppm */
rfst = (compC*4095.0*pwC*4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35));
rfst = (int) (rfst + 0.5);
if ( 1.0/(4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35)) < pwC )
{ text_error( " Not enough C13 RF. pwC must be %f usec or less.\n",
(1.0e6/(4000.0*sqrt((30.0*sfrq/600.0+7.0)/0.35))) ); psg_abort(1); }
}
}
else /* if autocal = 'y'(yes), 'q'(quiet), r(read), or 's'(semi) */
{
if(FIRST_FID) /* call Pbox */
{
if (C13refoc[A]=='y')
{
ppm = getval("dfrq"); ofs = 0.0; pws = 0.001; /* 1 ms long pulse */
bw = 200.0*ppm; nst = 1000; /* nst - number of steps */
stC200 = pbox_makeA("stC200A", "sech", bw, pws, ofs, compC*pwC, pwClvl, nst);
C13ofs = 100.0;
}
ofs_check(H1ofs, C13ofs, N15ofs, H2ofs);
}
rfst = stC200.pwrf;
}
/* selective H20 one-lobe sinc pulse needs 1.69 */
pwHs = getval("pwHs"); /* times more power than a square pulse */
if (pwHs > 1e-6) tpwrs = tpwr - 20.0*log10(pwHs/(compH*pw*1.69));
else tpwrs = 0.0;
tpwrs = (int) (tpwrs);
/* check validity of parameter range */
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y' ))
{ text_error("incorrect Dec1 decoupler flags! "); psg_abort(1); }
if((dm2[A] == 'y' || dm2[B] == 'y') )
{ text_error("incorrect Dec2 decoupler flags! "); psg_abort(1); }
if( dpwr > 0 )
{ text_error("don't fry the probe, dpwr too large! "); psg_abort(1); }
if( dpwr2 > 50 )
{ text_error("don't fry the probe, dpwr2 too large! "); psg_abort(1); }
if ((TROSY[A]=='y') && (dm2[C] == 'y'))
{ text_error("Choose either TROSY='n' or dm2='n' ! "); psg_abort(1); }
/* LOAD VARIABLES */
if(ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5);
tau1 = d2/2.0;
/* LOAD PHASE TABLES */
settable(t6, 4, recT);
if (TROSY[A] == 'y')
{ gsign = -1.0;
pwNt = pwN;
assign(zero,v7);
assign(two,v8);
settable(t1, 1, phT1);
settable(t2, 4, phT2);
settable(t3, 1, phT4);
settable(t4, 1, phT4);
settable(t5, 4, recT); }
else
{ assign(one,v7);
assign(three,v8);
settable(t1, 4, phi1);
settable(t2, 2, phi2);
settable(t3, 8, phi3);
settable(t4, 16, phi4);
settable(t5, 8, rec); }
if ( phase1 == 2 ) /* Hypercomplex in t1 */
{ if (TROSY[A] == 'y')
{ tsadd(t3, 2, 4); tsadd(t5, 2, 4); }
else tsadd(t2, 1, 4); }
if(t1_counter %2) /* calculate modification to phases based on */
{ tsadd(t2,2,4); tsadd(t5,2,4); tsadd(t6,2,4); } /* current t1 values */
if(wtg3919[0] != 'y')
{ add(one,v7,v7); add(one,v8,v8); }
/* sequence starts!! */
status(A);
obspower(tpwr);
dec2power(pwNlvl);
decpower(pwClvl);
decpwrf(rfst);
delay(d1);
status(B);
/* slective excitation of water */
rgpulse(pw, zero, rof1, rof1);
zgradpulse(gzlvl1,gt1);
obspower(tpwrs+6); /* make it a 180 inversion pulse */
shaped_pulse("H2Osinc", pwHs, zero, rof1, 0.0);
obspower(tpwr);
zgradpulse(gzlvl1,gt1);
/* CLEANEX-PM spin-lock */
if (cycles > 1.5000)
{
obspower(p1lvl);
txphase(zero);
rgpulse(pw135, zero, 0.0, 0.0);
rgpulse(pw120, two, 0.0, 0.0);
rgpulse(pw110, zero, 0.0, 0.0);
rgpulse(pw110, two, 0.0, 0.0);
rgpulse(pw120, zero, 0.0, 0.0);
rgpulse(pw135, two, 0.0, 0.0);
rgradient('z',gztm/4.0);
rgpulse(pw135, zero, 0.0, 0.0);
rgpulse(pw120, two, 0.0, 0.0);
rgpulse(pw110, zero, 0.0, 0.0);
rgpulse(pw110, two, 0.0, 0.0);
rgpulse(pw120, zero, 0.0, 0.0);
rgpulse(pw135, two, 0.0, 0.0);
rgradient('z',gztm/2.0);
rgpulse(pw135, zero, 0.0, 0.0);
rgpulse(pw120, two, 0.0, 0.0);
rgpulse(pw110, zero, 0.0, 0.0);
rgpulse(pw110, two, 0.0, 0.0);
rgpulse(pw120, zero, 0.0, 0.0);
rgpulse(pw135, two, 0.0, 0.0);
rgpulse(pw135, zero, 0.0, 0.0);
rgpulse(pw120, two, 0.0, 0.0);
rgpulse(pw110, zero, 0.0, 0.0);
rgpulse(pw110, two, 0.0, 0.0);
rgpulse(pw120, zero, 0.0, 0.0);
rgpulse(pw135, two, 0.0, 0.0);
rgradient('z',gztm/4.0*3.0);
rgpulse(pw135, zero, 0.0, 0.0);
rgpulse(pw120, two, 0.0, 0.0);
rgpulse(pw110, zero, 0.0, 0.0);
rgpulse(pw110, two, 0.0, 0.0);
rgpulse(pw120, zero, 0.0, 0.0);
rgpulse(pw135, two, 0.0, 0.0);
rgradient('z',gztm);
starthardloop(v10);
rgpulse(pw135, zero, 0.0, 0.0);
rgpulse(pw120, two, 0.0, 0.0);
rgpulse(pw110, zero, 0.0, 0.0);
rgpulse(pw110, two, 0.0, 0.0);
rgpulse(pw120, zero, 0.0, 0.0);
rgpulse(pw135, two, 0.0, 0.0);
endhardloop();
rgradient('z',gztm/4.0*3.0);
rgpulse(pw135, zero, 0.0, 0.0);
rgpulse(pw120, two, 0.0, 0.0);
rgpulse(pw110, zero, 0.0, 0.0);
rgpulse(pw110, two, 0.0, 0.0);
rgpulse(pw120, zero, 0.0, 0.0);
rgpulse(pw135, two, 0.0, 0.0);
rgradient('z',gztm/2.0);
rgpulse(pw135, zero, 0.0, 0.0);
rgpulse(pw120, two, 0.0, 0.0);
rgpulse(pw110, zero, 0.0, 0.0);
rgpulse(pw110, two, 0.0, 0.0);
rgpulse(pw120, zero, 0.0, 0.0);
rgpulse(pw135, two, 0.0, 0.0);
rgradient('z',gztm/4.0);
rgpulse(pw135, zero, 0.0, 0.0);
rgpulse(pw120, two, 0.0, 0.0);
rgpulse(pw110, zero, 0.0, 0.0);
rgpulse(pw110, two, 0.0, 0.0);
rgpulse(pw120, zero, 0.0, 0.0);
rgpulse(pw135, two, 0.0, 0.0);
rgradient('z', 0.0);
rgpulse(pw135, zero, 0.0, 0.0);
rgpulse(pw120, two, 0.0, 0.0);
rgpulse(pw110, zero, 0.0, 0.0);
rgpulse(pw110, two, 0.0, 0.0);
rgpulse(pw120, zero, 0.0, 0.0);
rgpulse(pw135, two, 0.0, 0.0);
obspower(tpwr);
}
/* ....................................... */
zgradpulse(0.3*gzlvl3,gt3);
txphase(zero);
dec2phase(zero);
delay(tauxh-gt3); /* delay=1/4J(XH) */
sim3pulse(2*pw,0.0,2*pwN,t4,zero,zero,rof1,rof1);
zgradpulse(0.3*gzlvl3,gt3);
dec2phase(t2);
delay(tauxh-gt3 ); /* delay=1/4J(XH) */
rgpulse(pw, t1, rof1, rof1);
zgradpulse(0.5*gsign*gzlvl3,gt3);
delay(200.0e-6);
decphase(zero);
if (TROSY[A] == 'y')
{
txphase(t3);
if ( phase1 == 2 )
dec2rgpulse(pwN, t6, rof1, 0.0);
else
dec2rgpulse(pwN, t2, rof1, 0.0);
if ( (C13refoc[A]=='y') && (d2 > 1.0e-3 + 2.0*WFG2_START_DELAY) )
{
delay(d2/2.0 - 0.5e-3 - WFG2_START_DELAY);
decshaped_pulse("stC200A", 1.0e-3, zero, 0.0, 0.0);
delay(d2/2.0 - 0.5e-3 - WFG2_STOP_DELAY);
}
else
delay(d2);
rgpulse(pw, t3, 0.0, rof1);
zgradpulse(0.3*gzlvl3,gt3);
delay(tauxh-gt3 );
sim3pulse(2*pw,0.0,2*pwN,zero,zero,zero,rof1,rof1);
zgradpulse(0.3*gzlvl3,gt3);
delay(tauxh-gt3 );
sim3pulse(pw,0.0,pwN,zero,zero,t3,rof1,rof1);
}
else
{
txphase(t4);
dec2rgpulse(pwN, t2, rof1, 0.0);
if ( (C13refoc[A]=='y') && (tau1 > 0.5e-3 + WFG2_START_DELAY) )
{
delay(tau1 - 0.5e-3 - WFG2_START_DELAY);
simshaped_pulse("", "stC200A", 2.0*pw, 1.0e-3, t4, zero, 0.0, 0.0);
dec2phase(t3);
delay(tau1 - 0.5e-3 - WFG2_STOP_DELAY);
}
else
{
tau1 -= pw;
if (tau1 < 0.0) tau1 = 0.0;
delay(tau1);
rgpulse(2.0*pw, t4, 0.0, 0.0);
dec2phase(t3);
delay(tau1);
}
dec2rgpulse(pwN, t3, 0.0, 0.0);
zgradpulse(0.5*gzlvl3,gt3);
delay(200.0e-6);
rgpulse(pw, two, rof1, rof1);
}
zgradpulse(gzlvl3,gt3);
txphase(v7); dec2phase(zero);
delay(tauxh-gt3-pwHs-rof1);
if(wtg3919[0] == 'y')
{
rgpulse(pw*0.231,v7,rof1,rof1);
delay(d3);
rgpulse(pw*0.692,v7,rof1,rof1);
delay(d3);
rgpulse(pw*1.462,v7,rof1,rof1);
delay(d3/2-pwN);
dec2rgpulse(2*pwN, zero, rof1, rof1);
txphase(v8);
delay(d3/2-pwN);
rgpulse(pw*1.462,v8,rof1,rof1);
delay(d3);
rgpulse(pw*0.692,v8,rof1,rof1);
delay(d3);
rgpulse(pw*0.231,v8,rof1,rof1);
}
else
{
obspower(tpwrs);
shaped_pulse("H2Osinc", pwHs, v7, rof1, 0.0);
obspower(tpwr);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, v8, zero, zero, 0.0, 0.0);
obspower(tpwrs);
shaped_pulse("H2Osinc", pwHs, v7, rof1, 0.0);
obspower(tpwr);
}
zgradpulse(gzlvl3,gt3);
delay(tauxh-gt3-pwHs-rof1-pwNt-POWER_DELAY);
dec2rgpulse(pwNt, zero, rof1, rof1);
dec2power(dpwr2);
status(C);
setreceiver(t5);
}
