void MappedSegmentMapDupeTest::createCallBack(AlignmentPtr alignment)
{
vector<Sequence::Info> seqVec(1);
BottomSegmentIteratorPtr bi;
BottomSegmentStruct bs;
TopSegmentIteratorPtr ti;
TopSegmentStruct ts;
// setup simple case were there is an edge from a parent to
// child and it is reversed
Genome* parent = alignment->addRootGenome("parent");
Genome* child1 = alignment->addLeafGenome("child1", "parent", 1);
Genome* child2 = alignment->addLeafGenome("child2", "parent", 1);
seqVec[0] = Sequence::Info("Sequence", 3, 0, 1);
parent->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 9, 3, 0);
child1->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 9, 3, 0);
child2->setDimensions(seqVec);
parent->setString("CCC");
child1->setString("CCCTACGTG");
child2->setString("CCCTACGTG");
bi = parent->getBottomSegmentIterator();
bs.set(0, 3);
bs._children.push_back(pair<hal_size_t, bool>(0, true));
bs._children.push_back(pair<hal_size_t, bool>(0, false));
bs.applyTo(bi);
ti = child1->getTopSegmentIterator();
ts.set(0, 3, 0, true, NULL_INDEX, 1);
ts.applyTo(ti);
ti->toRight();
ts.set(3, 3, 0, true, NULL_INDEX, 2);
ts.applyTo(ti);
ti->toRight();
ts.set(6, 3, 0, true, NULL_INDEX, 0);
ts.applyTo(ti);
ti = child2->getTopSegmentIterator();
ts.set(0, 3, 0, false);
ts.applyTo(ti);
ti->toRight();
ts.set(3, 3, NULL_INDEX, true);
ts.applyTo(ti);
ti->toRight();
ts.set(6, 3, NULL_INDEX, false);
ts.applyTo(ti);
}
void GenomeCreateTest::createCallBack(AlignmentPtr alignment)
{
hal_size_t alignmentSize = alignment->getNumGenomes();
CuAssertTrue(_testCase, alignmentSize == 0);
Genome* ancGenome = alignment->addRootGenome("AncGenome", 0);
Genome* leaf1Genome = alignment->addLeafGenome("Leaf1", "AncGenome", 0.1);
Genome* leaf2Genome = alignment->addLeafGenome("Leaf2", "AncGenome", 0.2);
Genome* leaf3Genome = alignment->addLeafGenome("Leaf3", "AncGenome", 0.3);
MetaData* ancMeta = ancGenome->getMetaData();
ancMeta->set("Young", "Jeezy");
vector<Sequence::Info> seqVec(1);
seqVec[0] =Sequence::Info("Sequence", 1000000, 5000, 700000);
ancGenome->setDimensions(seqVec);
seqVec[0] =Sequence::Info("Sequence", 1000000, 700000, 0);
leaf1Genome->setDimensions(seqVec);
seqVec[0] =Sequence::Info("Sequence", 2000000, 700000, 0);
leaf2Genome->setDimensions(seqVec);
seqVec[0] =Sequence::Info("Sequence", 3000000, 700000, 0);
leaf3Genome->setDimensions(seqVec);
}
void TopSegmentIteratorReverseTest::createCallBack(AlignmentPtr alignment)
{
vector<Sequence::Info> seqVec(1);
BottomSegmentIteratorPtr bi;
BottomSegmentStruct bs;
TopSegmentIteratorPtr ti;
TopSegmentStruct ts;
// setup simple case were there is an edge from a parent to
// child and it is reversed
Genome* parent1 = alignment->addRootGenome("parent1");
Genome* child1 = alignment->addLeafGenome("child1", "parent1", 1);
seqVec[0] = Sequence::Info("Sequence", 10, 2, 2);
parent1->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 10, 2, 2);
child1->setDimensions(seqVec);
parent1->setString("CCCTACGTGC");
child1->setString("CCCTACGTGC");
bi = parent1->getBottomSegmentIterator();
bs.set(0, 10, 0);
bs._children.push_back(pair<hal_size_t, bool>(0, true));
bs.applyTo(bi);
ti = child1->getTopSegmentIterator();
ts.set(0, 10, 0, true, 0);
ts.applyTo(ti);
bi = child1->getBottomSegmentIterator();
bs.set(0, 5, 0);
bs._children.clear();
bs.applyTo(bi);
bi->toRight();
bs.set(5, 5, 0);
bs.applyTo(bi);
}
void TopSegmentSimpleIteratorTest::createCallBack(AlignmentPtr alignment)
{
Genome* ancGenome = alignment->addRootGenome("Anc0", 0);
size_t numChildren = 9;
for (size_t i = 0; i < numChildren; ++i)
{
std::stringstream ss;
ss << i;
alignment->addLeafGenome(string("Leaf") + ss.str(), "Anc0", 0.1);
}
vector<Sequence::Info> seqVec(1);
seqVec[0] = Sequence::Info("Sequence", 1000000, 5000, 10000);
ancGenome->setDimensions(seqVec);
CuAssertTrue(_testCase, ancGenome->getNumChildren() == numChildren);
_topSegments.clear();
for (size_t i = 0; i < ancGenome->getNumTopSegments(); ++i)
{
TopSegmentStruct topSeg;
topSeg.setRandom();
topSeg._length =
ancGenome->getSequenceLength() / ancGenome->getNumTopSegments();
topSeg._startPosition = i * topSeg._length;
_topSegments.push_back(topSeg);
}
TopSegmentIteratorPtr tsIt = ancGenome->getTopSegmentIterator(0);
TopSegmentIteratorConstPtr tsEnd =
ancGenome->getTopSegmentEndIterator();
for (size_t i = 0; tsIt != tsEnd; tsIt->toRight(), ++i)
{
CuAssertTrue(_testCase,
(size_t)tsIt->getTopSegment()->getArrayIndex() == i);
_topSegments[i].applyTo(tsIt);
}
}
int main(int argc, char *argv[])
{
CLParserPtr optParser = initParser();
string inPath, botAlignmentPath, topAlignmentPath, parentName, insertName,
childName, leafName;
double upperBranchLength, leafBranchLength;
bool noMarkAncestors;
try {
optParser->parseOptions(argc, argv);
inPath = optParser->getArgument<string>("inFile");
botAlignmentPath = optParser->getArgument<string>("botAlignmentFile");
topAlignmentPath = optParser->getArgument<string>("topAlignmentFile");
parentName = optParser->getArgument<string>("parentName");
insertName = optParser->getArgument<string>("insertName");
childName = optParser->getArgument<string>("childName");
leafName = optParser->getArgument<string>("leafName");
upperBranchLength = optParser->getArgument<double>("upperBranchLength");
leafBranchLength = optParser->getArgument<double>("leafBranchLength");
noMarkAncestors = optParser->getFlag("noMarkAncestors");
} catch (exception &e) {
optParser->printUsage(cerr);
return 1;
}
AlignmentPtr mainAlignment = openHalAlignment(inPath, optParser);
AlignmentConstPtr botAlignment = openHalAlignment(botAlignmentPath,
optParser);
AlignmentConstPtr topAlignment = openHalAlignment(topAlignmentPath,
optParser);
mainAlignment->insertGenome(insertName, parentName, childName,
upperBranchLength);
mainAlignment->addLeafGenome(leafName, insertName, leafBranchLength);
// Insert the new intermediate node.
Genome *insertGenome = mainAlignment->openGenome(insertName);
const Genome *topInsertGenome = topAlignment->openGenome(insertName);
const Genome *botInsertGenome = botAlignment->openGenome(insertName);
topInsertGenome->copyDimensions(insertGenome);
topInsertGenome->copyTopDimensions(insertGenome);
botInsertGenome->copyBottomDimensions(insertGenome);
topInsertGenome->copySequence(insertGenome);
topInsertGenome->copyTopSegments(insertGenome);
topInsertGenome->copyMetadata(insertGenome);
botInsertGenome->copyBottomSegments(insertGenome);
insertGenome->fixParseInfo();
// Copy the bottom segments for the parent genome from the top alignment.
Genome *parentGenome = mainAlignment->openGenome(parentName);
const Genome *botParentGenome = topAlignment->openGenome(parentName);
botParentGenome->copyBottomDimensions(parentGenome);
botParentGenome->copyBottomSegments(parentGenome);
parentGenome->fixParseInfo();
// Fix the parent's other children as well.
vector<string> allChildren = mainAlignment->getChildNames(parentName);
for (size_t i = 0; i < allChildren.size(); i++)
{
if (allChildren[i] != insertName)
{
Genome *outGenome = mainAlignment->openGenome(allChildren[i]);
const Genome *topSegmentsGenome = topAlignment->openGenome(allChildren[i]);
topSegmentsGenome->copyTopDimensions(outGenome);
topSegmentsGenome->copyTopSegments(outGenome);
outGenome->fixParseInfo();
}
}
// Copy the top segments for the child genome from the bottom alignment.
Genome *childGenome = mainAlignment->openGenome(childName);
const Genome *topChildGenome = botAlignment->openGenome(childName);
topChildGenome->copyTopDimensions(childGenome);
topChildGenome->copyTopSegments(childGenome);
childGenome->fixParseInfo();
// Copy the entire genome for the leaf from the bottom alignment.
Genome *outLeafGenome = mainAlignment->openGenome(leafName);
const Genome *inLeafGenome = botAlignment->openGenome(leafName);
inLeafGenome->copy(outLeafGenome);
if (!noMarkAncestors) {
markAncestorsForUpdate(mainAlignment, insertName);
}
mainAlignment->close();
botAlignment->close();
topAlignment->close();
}
void TopSegmentIsGapTest::createCallBack(AlignmentPtr alignment)
{
size_t numSequences = 3;
vector<Sequence::Info> seqVec(numSequences);
BottomSegmentIteratorPtr bi;
BottomSegmentStruct bs;
TopSegmentIteratorPtr ti;
TopSegmentStruct ts;
Genome* parent1 = alignment->addRootGenome("parent1");
Genome* child1 = alignment->addLeafGenome("child1", "parent1", 1);
// set up two genomes. each with three sequences. each sequence
// with 5 segments of length two. start with segment i in parent
// aligned with segment i in child.
for (size_t i = 0; i < numSequences; ++i)
{
stringstream ss;
ss << "Sequence" << i;
string name = ss.str();
seqVec[i] = Sequence::Info(name, 10, 5, 5);
}
parent1->setDimensions(seqVec);
child1->setDimensions(seqVec);
bi = parent1->getBottomSegmentIterator();
for (; bi != parent1->getBottomSegmentEndIterator(); bi->toRight())
{
bs.set(bi->getBottomSegment()->getArrayIndex() * 2, 2);
bs._children.clear();
bs._children.push_back(pair<hal_size_t, bool>(
bi->getBottomSegment()->getArrayIndex(),
false));
bs.applyTo(bi);
}
ti = child1->getTopSegmentIterator();
for (; ti != child1->getTopSegmentEndIterator(); ti->toRight())
{
ts.set(ti->getTopSegment()->getArrayIndex() * 2, 2,
ti->getTopSegment()->getArrayIndex());
ts.applyTo(ti);
}
// insertion in middle (8th top segment)
bi = parent1->getBottomSegmentIterator(8);
ti = child1->getTopSegmentIterator(8);
assert(bi->getBottomSegment()->getChildIndex(0) == 8 &&
ti->getTopSegment()->getParentIndex() == 8);
bi->getBottomSegment()->setChildIndex(0, 9);
ti->getTopSegment()->setParentIndex(NULL_INDEX);
ti->toRight();
ti->getTopSegment()->setParentIndex(8);
// insertion at begining (10th top segment)
bi = parent1->getBottomSegmentIterator(10);
ti = child1->getTopSegmentIterator(10);
assert(bi->getBottomSegment()->getChildIndex(0) == 10 &&
ti->getTopSegment()->getParentIndex() == 10);
bi->getBottomSegment()->setChildIndex(0, 11);
ti->getTopSegment()->setParentIndex(NULL_INDEX);
ti->toRight();
ti->getTopSegment()->setParentIndex(10);
// just having a null parent is not enough for an insertion
bi = parent1->getBottomSegmentIterator(2);
ti = child1->getTopSegmentIterator(2);
assert(bi->getBottomSegment()->getChildIndex(0) == 2 &&
ti->getTopSegment()->getParentIndex() == 2);
ti->getTopSegment()->setParentIndex(NULL_INDEX);
}
void MappedSegmentMapExtraParalogsTest::createCallBack(AlignmentPtr alignment)
{
vector<Sequence::Info> seqVec(1);
BottomSegmentIteratorPtr bi;
BottomSegmentStruct bs;
TopSegmentIteratorPtr ti;
TopSegmentStruct ts;
// Set up a case where all the segments of grandChild1 coalesce with
// the first segment of grandChild2, but only if using the root as
// the coalescence limit. Otherwise only the first segments map to
// each other.
Genome* root = alignment->addRootGenome("root");
Genome* parent = alignment->addLeafGenome("parent", "root", 1);
Genome* grandChild1 = alignment->addLeafGenome("grandChild1", "parent", 1);
Genome* grandChild2 = alignment->addLeafGenome("grandChild2", "parent", 1);
seqVec[0] = Sequence::Info("Sequence", 3, 0, 1);
root->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 9, 3, 3);
parent->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 9, 3, 0);
grandChild1->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 9, 3, 0);
grandChild2->setDimensions(seqVec);
root->setString("CCC");
parent->setString("CCCTACGTG");
grandChild1->setString("CCCTACGTG");
grandChild2->setString("CCCTACGTG");
bi = root->getBottomSegmentIterator();
bs.set(0, 3);
bs._children.push_back(pair<hal_size_t, bool>(0, false));
bs.applyTo(bi);
ti = parent->getTopSegmentIterator();
ts.set(0, 3, 0, false, NULL_INDEX, 1);
ts.applyTo(ti);
ti->toRight();
ts.set(3, 3, 0, false, NULL_INDEX, 2);
ts.applyTo(ti);
ti->toRight();
ts.set(6, 3, 0, false, NULL_INDEX, 0);
ts.applyTo(ti);
bi = parent->getBottomSegmentIterator();
bs.set(0, 3);
bs._children.clear();
bs._children.push_back(pair<hal_size_t, bool>(0, true));
bs._children.push_back(pair<hal_size_t, bool>(0, false));
bs.applyTo(bi);
bi->toRight();
bs.set(3, 3);
bs._children.clear();
bs._children.push_back(pair<hal_size_t, bool>(1, true));
bs._children.push_back(pair<hal_size_t, bool>(NULL_INDEX, true));
bs.applyTo(bi);
bi->toRight();
bs.set(6, 3);
bs._children.clear();
bs._children.push_back(pair<hal_size_t, bool>(2, true));
bs._children.push_back(pair<hal_size_t, bool>(NULL_INDEX, false));
bs.applyTo(bi);
ti = grandChild1->getTopSegmentIterator();
ts.set(0, 3, 0, true);
ts.applyTo(ti);
ti->toRight();
ts.set(3, 3, 1, true);
ts.applyTo(ti);
ti->toRight();
ts.set(6, 3, 2, true);
ts.applyTo(ti);
ti = grandChild2->getTopSegmentIterator();
ts.set(0, 3, 0, false);
ts.applyTo(ti);
ti->toRight();
ts.set(3, 3, NULL_INDEX, true);
ts.applyTo(ti);
ti->toRight();
ts.set(6, 3, NULL_INDEX, false);
ts.applyTo(ti);
parent->fixParseInfo();
}
void MappedSegmentMapUpTest::createCallBack(AlignmentPtr alignment)
{
vector<Sequence::Info> seqVec(1);
BottomSegmentIteratorPtr bi;
BottomSegmentStruct bs;
TopSegmentIteratorPtr ti;
TopSegmentStruct ts;
// setup simple case were there is an edge from a parent to
// child1 and it is reversed and nonreversed to child2
Genome* parent = alignment->addRootGenome("parent");
Genome* child1 = alignment->addLeafGenome("child1", "parent", 1);
Genome* child2 = alignment->addLeafGenome("child2", "parent", 1);
// add a bunch of grandchildren with no rearrangemnts to test
// simple parsing
Genome* g1 = alignment->addLeafGenome("g1", "child2", 1);
Genome* g2 = alignment->addLeafGenome("g2", "g1", 1);
Genome* g3 = alignment->addLeafGenome("g3", "g2", 1);
Genome* g4 = alignment->addLeafGenome("g4", "g3", 1);
Genome* g5 = alignment->addLeafGenome("g5", "g4", 1);
// add some with random inversions
Genome* gi1 = alignment->addLeafGenome("gi1", "child1", 1);
Genome* gi2 = alignment->addLeafGenome("gi2", "gi1", 1);
Genome* gi3 = alignment->addLeafGenome("gi3", "gi2", 1);
Genome* gi4 = alignment->addLeafGenome("gi4", "gi3", 1);
Genome* gi5 = alignment->addLeafGenome("gi5", "gi4", 1);
Genome* gs[] = {g1, g2, g3, g4, g5};
Genome* gis[] = {gi1, gi2, gi3, gi4, gi5};
seqVec[0] = Sequence::Info("Sequence", 12, 0, 1);
parent->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 1, 6);
child1->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 1, 6);
child2->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 6, 4);
g1->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 4, 3);
g2->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 3, 2);
g3->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 2, 12);
g4->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 12, 0);
g5->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 6, 4);
gi1->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 4, 3);
gi2->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 3, 2);
gi3->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 2, 12);
gi4->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 12, 12, 0);
gi5->setDimensions(seqVec);
parent->setString("CCCTACTTGTGC");
child1->setString("CCCTACTTGTGC");
child2->setString("CCCTACTTGTGC");
for (size_t i = 0; i < 5; ++i)
{
gs[i]->setString("TCCTACTTGTGC");
gis[i]->setString("TCCTACTTGTGC");
}
bi = parent->getBottomSegmentIterator();
bs.set(0, 12);
bs._children.push_back(pair<hal_size_t, bool>(0, true));
bs._children.push_back(pair<hal_size_t, bool>(0, false));
bs.applyTo(bi);
ti = child1->getTopSegmentIterator();
ts.set(0, 12, 0, true, 0);
ts.applyTo(ti);
ti = child2->getTopSegmentIterator();
ts.set(0, 12, 0, false, 0);
ts.applyTo(ti);
for (size_t i = 0; i < 6; ++i)
{
bi = child2->getBottomSegmentIterator(i);
bs.set(i * 2, 2, 0);
bs._children.clear();
bs._children.push_back(pair<hal_size_t, bool>(i, false));
bs.applyTo(bi);
ti = g1->getTopSegmentIterator(i);
ts.set(i * 2, 2, i, false);
ts.applyTo(ti);
}
for (size_t i = 0; i < 6; ++i)
{
bi = child1->getBottomSegmentIterator(i);
bs.set(i * 2, 2, 0);
bs._children.clear();
bs._children.push_back(pair<hal_size_t, bool>(i, false));
bs.applyTo(bi);
ti = gi1->getTopSegmentIterator(i);
ts.set(i * 2, 2, i, false);
ts.applyTo(ti);
}
for (size_t i = 0; i < 5; ++i)
{
const Genome* g = gs[i];
const Genome* parent = g->getParent();
const Genome* child = i == 4 ? NULL : g->getChild(0);
hal_size_t segLen = g->getSequenceLength() / g->getNumTopSegments();
hal_size_t psegLen = parent->getSequenceLength() /
parent->getNumTopSegments();
hal_size_t csegLen = 0;
if (child)
{
csegLen = child->getSequenceLength() / child->getNumTopSegments();
}
for (size_t j = 0; j < g->getNumTopSegments(); ++j)
{
bool inv = false;
bi = parent->getBottomSegmentIterator(j);
bs.set(j * segLen, segLen, (j * segLen) / psegLen);
bs._children.clear();
bs._children.push_back(pair<hal_size_t, bool>(j, inv));
bs.applyTo(bi);
hal_index_t bparse = NULL_INDEX;
if (child != NULL)
{
bparse = (j * segLen) / csegLen;
}
ti = g->getTopSegmentIterator(j);
ts.set(j * segLen, segLen, j, inv, bparse);
ts.applyTo(ti);
}
}
for (size_t i = 0; i < 5; ++i)
{
const Genome* g = gis[i];
const Genome* parent = g->getParent();
const Genome* child = i == 4 ? NULL : g->getChild(0);
hal_size_t segLen = g->getSequenceLength() / g->getNumTopSegments();
hal_size_t psegLen = parent->getSequenceLength() /
parent->getNumTopSegments();
hal_size_t csegLen = 0;
if (child)
{
csegLen = child->getSequenceLength() / child->getNumTopSegments();
}
for (size_t j = 0; j < g->getNumTopSegments(); ++j)
{
bool inv = rand() % 4 == 0;
bi = parent->getBottomSegmentIterator(j);
bs.set(j * segLen, segLen, (j * segLen) / psegLen);
bs._children.clear();
bs._children.push_back(pair<hal_size_t, bool>(j, inv));
bs.applyTo(bi);
hal_index_t bparse = NULL_INDEX;
if (child != NULL)
{
bparse = (j * segLen) / csegLen;
}
ti = g->getTopSegmentIterator(j);
ts.set(j * segLen, segLen, j, inv, bparse);
ts.applyTo(ti);
}
}
}