Beispiel #1
0
int bamfiltermc(libmaus2::util::ArgInfo const & arginfo)
{
	bool const verbose = arginfo.getValue("verbose",getDefaultVerbose());

	libmaus2::bambam::BamAlignmentDecoderWrapper::unique_ptr_type decwrapper(
		libmaus2::bambam::BamMultiAlignmentDecoderFactory::construct(arginfo));
	::libmaus2::bambam::BamAlignmentDecoder * ppdec = &(decwrapper->getDecoder());
	::libmaus2::bambam::BamAlignmentDecoder & dec = *ppdec;
	::libmaus2::bambam::BamHeader const & header = dec.getHeader();
	::libmaus2::bambam::BamAlignment & algn = dec.getAlignment();
	std::string const tmpfilenamebase = arginfo.getUnparsedValue("tmpfile",arginfo.getDefaultTmpFileName());
	uint64_t const numthreads = arginfo.getValueUnsignedNumeric<uint64_t>("numthreads",getDefaultNumThreads());

	/*
	 * start index/md5 callbacks
	 */
	std::string const tmpfileindex = tmpfilenamebase + "_index";
	::libmaus2::util::TempFileRemovalContainer::addTempFile(tmpfileindex);

	std::string md5filename;
	std::string indexfilename;

	std::vector< ::libmaus2::lz::BgzfDeflateOutputCallback * > cbs;
	::libmaus2::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Pmd5cb;
	if ( arginfo.getValue<unsigned int>("md5",getDefaultMD5()) )
	{
		if ( libmaus2::bambam::BamBlockWriterBaseFactory::getMD5FileName(arginfo) != std::string() )
			md5filename = libmaus2::bambam::BamBlockWriterBaseFactory::getMD5FileName(arginfo);
		else
			std::cerr << "[V] no filename for md5 given, not creating hash" << std::endl;

		if ( md5filename.size() )
		{
			::libmaus2::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Tmd5cb(new ::libmaus2::lz::BgzfDeflateOutputCallbackMD5);
			Pmd5cb = UNIQUE_PTR_MOVE(Tmd5cb);
			cbs.push_back(Pmd5cb.get());
		}
	}
	libmaus2::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Pindex;
	if ( arginfo.getValue<unsigned int>("index",getDefaultIndex()) )
	{
		if ( libmaus2::bambam::BamBlockWriterBaseFactory::getIndexFileName(arginfo) != std::string() )
			indexfilename = libmaus2::bambam::BamBlockWriterBaseFactory::getIndexFileName(arginfo);
		else
			std::cerr << "[V] no filename for index given, not creating index" << std::endl;

		if ( indexfilename.size() )
		{
			libmaus2::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Tindex(new libmaus2::bambam::BgzfDeflateOutputCallbackBamIndex(tmpfileindex));
			Pindex = UNIQUE_PTR_MOVE(Tindex);
			cbs.push_back(Pindex.get());
		}
	}
	std::vector< ::libmaus2::lz::BgzfDeflateOutputCallback * > * Pcbs = 0;
	if ( cbs.size() )
		Pcbs = &cbs;
	/*
	 * end md5/index callbacks
	 */

	::libmaus2::bambam::BamHeader::unique_ptr_type genuphead(
		libmaus2::bambam::BamHeaderUpdate::updateHeader(arginfo,header,"bamfiltermc",std::string(PACKAGE_VERSION))
	);
	libmaus2::bambam::BamBlockWriterBase::unique_ptr_type Pwriter(libmaus2::bambam::BamBlockWriterBaseFactory::construct(*genuphead,arginfo,Pcbs));
	libmaus2::bambam::BamBlockWriterBase & wr = *Pwriter;

	// freelist size
	uint64_t const flsize = 16*1024;
	libmaus2::util::FreeList < libmaus2::bambam::BamAlignment, BamAlignmentFreeListDefaultAllocator, BamAlignmentFreeListDefaultTypeInfo > FL(flsize);
	libmaus2::util::SimpleQueue < libmaus2::bambam::BamAlignment::shared_ptr_type > Q;

	libmaus2::bambam::BamAuxFilterVector auxvec;
	auxvec.set('M','C');

	uint64_t alcnt = 0;
	while ( dec.readAlignment() )
	{
		if ( FL.empty() )
			handleQueue(Q,FL,wr,auxvec,numthreads);
		assert ( ! FL.empty() );

		libmaus2::bambam::BamAlignment::shared_ptr_type P = FL.get();
		P->swap(algn);

		Q.push_back(P);

		if ( verbose && ((++alcnt % (1024*1024)) == 0) )
			std::cerr << "[V] " << alcnt << std::endl;
	}

	handleQueue(Q,FL,wr,auxvec,numthreads);

	// reset BAM writer
	Pwriter.reset();

	if ( Pmd5cb )
		Pmd5cb->saveDigestAsFile(md5filename);
	if ( Pindex )
		Pindex->flush(std::string(indexfilename));

	return EXIT_SUCCESS;
}
Beispiel #2
0
int bamvalidateTemplate(::libmaus2::util::ArgInfo const & arginfo)
{
	libmaus2::timing::RealTimeClock rtc; rtc.start();
	bool const verbose = arginfo.getValue("verbose",getDefaultVerbose());
	bool const basequalhist = arginfo.getValue("basequalhist",getDefaultBaseQualHist());

	libmaus2::bambam::BamAlignmentDecoderWrapper::unique_ptr_type decwrapper(
		libmaus2::bambam::BamMultiAlignmentDecoderFactory::construct(
			arginfo,false // put rank
		)
	);
	::libmaus2::bambam::BamAlignmentDecoder * ppdec = &(decwrapper->getDecoder());
	::libmaus2::bambam::BamAlignmentDecoder & dec = *ppdec;
	::libmaus2::bambam::BamHeader const & header = dec.getHeader();
	::libmaus2::bambam::BamAlignment const & algn = dec.getAlignment();

	// add PG line to header
	std::string const upheadtext = ::libmaus2::bambam::ProgramHeaderLineSet::addProgramLine(
		header.text,
		"bamvalidate", // ID
		"bamvalidate", // PN
		arginfo.commandline, // CL
		::libmaus2::bambam::ProgramHeaderLineSet(header.text).getLastIdInChain(), // PP
		std::string(PACKAGE_VERSION) // VN
	);
	// construct new header
	::libmaus2::bambam::BamHeader uphead(upheadtext);

	/*
	 * start index/md5 callbacks and alignment writer
	 */
	std::string md5filename;
	std::string indexfilename;

	std::vector< ::libmaus2::lz::BgzfDeflateOutputCallback * > cbs;
	::libmaus2::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Pmd5cb;
	libmaus2::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Pindex;
	libmaus2::bambam::BamBlockWriterBase::unique_ptr_type Pout;

	if ( passthrough )
	{
		std::string const tmpfilenamebase = arginfo.getValue<std::string>("tmpfile",arginfo.getDefaultTmpFileName());
		std::string const tmpfileindex = tmpfilenamebase + "_index";
		::libmaus2::util::TempFileRemovalContainer::addTempFile(tmpfileindex);

		if ( arginfo.getValue<unsigned int>("md5",getDefaultMD5()) )
		{
			if ( arginfo.hasArg("md5filename") &&  arginfo.getUnparsedValue("md5filename","") != "" )
				md5filename = arginfo.getUnparsedValue("md5filename","");
			else
				std::cerr << "[V] no filename for md5 given, not creating hash" << std::endl;

			if ( md5filename.size() )
			{
				::libmaus2::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Tmd5cb(new ::libmaus2::lz::BgzfDeflateOutputCallbackMD5);
				Pmd5cb = UNIQUE_PTR_MOVE(Tmd5cb);
				cbs.push_back(Pmd5cb.get());
			}
		}
		if ( arginfo.getValue<unsigned int>("index",getDefaultIndex()) )
		{
			if ( arginfo.hasArg("indexfilename") &&  arginfo.getUnparsedValue("indexfilename","") != "" )
				indexfilename = arginfo.getUnparsedValue("indexfilename","");
			else
				std::cerr << "[V] no filename for index given, not creating index" << std::endl;

			if ( indexfilename.size() )
			{
				libmaus2::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Tindex(new libmaus2::bambam::BgzfDeflateOutputCallbackBamIndex(tmpfileindex));
				Pindex = UNIQUE_PTR_MOVE(Tindex);
				cbs.push_back(Pindex.get());
			}
		}
		std::vector< ::libmaus2::lz::BgzfDeflateOutputCallback * > * Pcbs = 0;
		if ( cbs.size() )
			Pcbs = &cbs;


		libmaus2::bambam::BamBlockWriterBase::unique_ptr_type Tout (
			libmaus2::bambam::BamBlockWriterBaseFactory::construct(uphead, arginfo, Pcbs)
		);
		Pout = UNIQUE_PTR_MOVE(Tout);
	}

	libmaus2::autoarray::AutoArray<char> lastvalidname(256); // max valid read name is 255 bytes
	uint64_t alsok = 0;

	::libmaus2::autoarray::AutoArray<char> qual;
	libmaus2::autoarray::AutoArray<uint64_t> H(static_cast<uint64_t>(std::numeric_limits<uint8_t>::max())+1);
	std::fill(H.begin(),H.end(),0ull);

	try
	{
		while ( dec.readAlignment() )
		{
			if ( passthrough )
				Pout->writeAlignment(algn);

			if ( basequalhist )
			{
				uint64_t const l = algn.getLseq();
				uint8_t const * Qc = libmaus2::bambam::BamAlignmentDecoderBase::getQual(algn.D.begin());
				uint8_t const * const Qe = Qc + l;

				while ( Qc != Qe )
					H[*(Qc++)]++;
			}

			uint64_t const lname = algn.getLReadName();
			char const * name = algn.getName();
			std::copy(name,name+lname+1,lastvalidname.begin());

			alsok += 1;
		}
	}
	catch(std::exception const & ex)
	{
		std::cerr << "[E] name of last valid alignment was " << lastvalidname.begin() << std::endl;
		std::cerr << "[E] read " << alsok << " valid alignments" << std::endl;
		throw;
	}

	Pout.reset();

	if ( Pmd5cb )
	{
		Pmd5cb->saveDigestAsFile(md5filename);
	}
	if ( Pindex )
	{
		Pindex->flush(std::string(indexfilename));
	}

	if ( verbose )
		std::cerr << "[V] checked " << alsok << " alignments in " << rtc.formatTime(rtc.getElapsedSeconds())
			<< " (" << alsok / rtc.getElapsedSeconds() << " al/s)" << std::endl;

	if ( basequalhist )
	{
		uint64_t const s = std::accumulate(H.begin(),H.end(),0ull);

		uint64_t a = 0;
		uint64_t minq = std::numeric_limits<uint64_t>::max();
		uint64_t maxq = 0;

		for ( uint64_t i = 0; i < H.size(); ++i )
			if ( H[i] )
			{
				minq = std::min(minq,i);
				maxq = std::max(maxq,i);

				a += H[i];

				std::cerr
					<< "[H]\t" << i << "\t";

				if ( ( static_cast<uint64_t>(i+33) < static_cast<uint64_t>(std::numeric_limits<char>::max()) && isprint(i+33)) )
					std::cerr << static_cast<char>(i+33);

				std::cerr << "\t"
					<< H[i] << "\t"
					<< (H[i] / static_cast<double>(s)) << "\t"
					<< (a / static_cast<double>(s))
					<< std::endl;
			}

		if ( s )
		{
			std::cerr << "[H]\tmin\t" << minq << "\t";
			if ( ( static_cast<uint64_t>(minq+33) < static_cast<uint64_t>(std::numeric_limits<char>::max()) && isprint(minq+33)) )
				std::cerr << static_cast<char>(minq+33);
			std::cerr << std::endl;
			std::cerr << "[H]\tmax\t" << maxq << "\t";
			if ( ( static_cast<uint64_t>(maxq+33) < static_cast<uint64_t>(std::numeric_limits<char>::max()) && isprint(maxq+33)) )
				std::cerr << static_cast<char>(maxq+33);
			std::cerr << std::endl;
		}
	}

	return EXIT_SUCCESS;
}
Beispiel #3
0
int bamheap2(libmaus2::util::ArgInfo const & arginfo)
{
	bool const verbose = arginfo.getValue("verbose",getDefaultVerbose());
	std::string const reference = arginfo.getUnparsedValue("reference",std::string());
	std::string const outputprefix = arginfo.getUnparsedValue("outputprefix",std::string());

	libmaus2::bambam::BamAlignmentDecoderWrapper::unique_ptr_type decwrapper(
		libmaus2::bambam::BamMultiAlignmentDecoderFactory::construct(arginfo));
	::libmaus2::bambam::BamAlignmentDecoder * ppdec = &(decwrapper->getDecoder());
	::libmaus2::bambam::BamAlignmentDecoder & dec = *ppdec;
	::libmaus2::bambam::BamHeader const & header = dec.getHeader();
	::libmaus2::bambam::BamAlignment const & algn = dec.getAlignment();

	double const damult = arginfo.getValue<double>("amult",1);
	double const dcmult = arginfo.getValue<double>("cmult",1);
	double const dgmult = arginfo.getValue<double>("gmult",1);
	double const dtmult = arginfo.getValue<double>("tmult",1);
	double const dpadmult = arginfo.getValue<double>("padmult",1);

	double maxmult = 0;
	maxmult = std::max(damult,maxmult);
	maxmult = std::max(dcmult,maxmult);
	maxmult = std::max(dgmult,maxmult);
	maxmult = std::max(dtmult,maxmult);
	maxmult = std::max(dpadmult,maxmult);

	uint64_t const amult = std::floor((damult / maxmult) * (1ull<<16) + 0.5);
	uint64_t const cmult = std::floor((dcmult / maxmult) * (1ull<<16) + 0.5);
	uint64_t const gmult = std::floor((dgmult / maxmult) * (1ull<<16) + 0.5);
	uint64_t const tmult = std::floor((dtmult / maxmult) * (1ull<<16) + 0.5);
	uint64_t const padmult = std::floor((dpadmult / maxmult) * (1ull<<16) + 0.5);

	libmaus2::fastx::FastAIndex::unique_ptr_type Pindex;
	libmaus2::aio::InputStreamInstance::unique_ptr_type PCIS;
	if ( reference.size() )
	{
		libmaus2::fastx::FastAIndex::unique_ptr_type Tindex(
			libmaus2::fastx::FastAIndex::load(reference+".fai")
		);
		Pindex = UNIQUE_PTR_MOVE(Tindex);

		libmaus2::aio::InputStreamInstance::unique_ptr_type TCIS(new libmaus2::aio::InputStreamInstance(reference));
		PCIS = UNIQUE_PTR_MOVE(TCIS);
	}

	libmaus2::autoarray::AutoArray<libmaus2::bambam::cigar_operation> cigop;
	libmaus2::autoarray::AutoArray<char> bases;

	int64_t prevrefid = -1;
	std::string refidname = "*";

	std::map< uint64_t, HeapEntry > M;
	uint64_t alcnt = 0;
	std::vector< std::pair<char,uint8_t> > pendinginserts;
	int64_t loadedRefId = -1;
	int64_t streamRefId = -1;
	libmaus2::autoarray::AutoArray<char> refseqbases;
	ConsensusAccuracy * consacc = 0;
	std::map<uint64_t,ConsensusAccuracy> Mconsacc;
	typedef libmaus2::util::shared_ptr<std::ostringstream>::type stream_ptr_type;
	stream_ptr_type Pstream;
	ConsensusAux Caux;

	Caux.M['a'] = Caux.M['A'] = amult;
	Caux.M['c'] = Caux.M['C'] = cmult;
	Caux.M['g'] = Caux.M['G'] = gmult;
	Caux.M['t'] = Caux.M['T'] = tmult;
	Caux.M[padsym] = padmult;

	while ( dec.readAlignment() )
	{
		if (
			algn.isMapped() && (!algn.isQCFail()) &&
			algn.getLseq()
		)
		{
			assert ( ! pendinginserts.size() );

			uint32_t const numcigop = algn.getCigarOperations(cigop);
			uint64_t readpos = 0;
			uint64_t refpos = algn.getPos();
			uint64_t const seqlen = algn.decodeRead(bases);
			uint8_t const * qual = libmaus2::bambam::BamAlignmentDecoderBase::getQual(algn.D.begin());

			// handle finished columns
			if ( algn.getRefID() != prevrefid )
			{
				while ( M.size() )
				{
					HeapEntry & H = M.begin()->second;

					if ( outputprefix.size() && (streamRefId != prevrefid) )
					{
						if ( Pstream )
						{
							std::ostringstream fnostr;
							fnostr << outputprefix << "_" << header.getRefIDName(streamRefId);
							libmaus2::aio::OutputStreamInstance PFOS(fnostr.str());
							PFOS << ">" << header.getRefIDName(streamRefId) << '\n';
							PFOS << Pstream->str() << '\n';

							Pstream.reset();
						}

						stream_ptr_type Tstream(new std::ostringstream);
						Pstream = Tstream;
						streamRefId = prevrefid;
					}

					if ( Pindex && (loadedRefId != prevrefid) )
					{
						refseqbases = Pindex->readSequence(*PCIS, Pindex->getSequenceIdByName(refidname));
						loadedRefId = prevrefid;

						if ( Mconsacc.find(loadedRefId) == Mconsacc.end() )
							Mconsacc[loadedRefId] = ConsensusAccuracy(refseqbases.size());

						consacc = &(Mconsacc[loadedRefId]);
					}

					H.toStream(std::cout,M.begin()->first,refidname,(M.begin()->first < refseqbases.size()) ? static_cast<int>(refseqbases[M.begin()->first]) : -1,Caux,consacc,Pstream.get());

					M.erase(M.begin());
				}

				prevrefid = algn.getRefID();
				refidname = header.getRefIDName(prevrefid);
			}
			else
			{
				while ( M.size() && M.begin()->first < refpos )
				{
					HeapEntry & H = M.begin()->second;

					if ( outputprefix.size() && (streamRefId != prevrefid) )
					{
						if ( Pstream )
						{
							std::ostringstream fnostr;
							fnostr << outputprefix << "_" << header.getRefIDName(streamRefId);
							libmaus2::aio::OutputStreamInstance PFOS(fnostr.str());
							PFOS << ">" << header.getRefIDName(streamRefId) << '\n';
							PFOS << Pstream->str() << '\n';

							Pstream.reset();
						}

						stream_ptr_type Tstream(new std::ostringstream);
						Pstream = Tstream;
						streamRefId = prevrefid;
					}

					if ( Pindex && (loadedRefId != prevrefid) )
					{
						refseqbases = Pindex->readSequence(*PCIS, Pindex->getSequenceIdByName(refidname));
						loadedRefId = prevrefid;

						if ( Mconsacc.find(loadedRefId) == Mconsacc.end() )
							Mconsacc[loadedRefId] = ConsensusAccuracy(refseqbases.size());

						consacc = &(Mconsacc[loadedRefId]);
					}

					H.toStream(std::cout,M.begin()->first,refidname,(M.begin()->first < refseqbases.size()) ? static_cast<int>(refseqbases[M.begin()->first]) : -1,Caux,consacc,Pstream.get());

					M.erase(M.begin());
				}
			}

			for ( uint64_t ci = 0; ci < numcigop; ++ci )
			{
				uint64_t const ciglen = cigop[ci].second;

				switch ( cigop[ci].first )
				{
					case libmaus2::bambam::BamFlagBase::LIBMAUS2_BAMBAM_CMATCH:
					case libmaus2::bambam::BamFlagBase::LIBMAUS2_BAMBAM_CEQUAL:
					case libmaus2::bambam::BamFlagBase::LIBMAUS2_BAMBAM_CDIFF:
					{
						if ( pendinginserts.size() )
						{
							M[refpos].I.push_back(pendinginserts);
							pendinginserts.resize(0);
						}

						for ( uint64_t i = 0; i < ciglen; ++i )
						{
							M[refpos].V.push_back(std::make_pair(bases[readpos],qual[readpos]));
							readpos++;
							refpos++;
						}
						break;
					}
					case libmaus2::bambam::BamFlagBase::LIBMAUS2_BAMBAM_CINS:
					{
						for ( uint64_t i = 0; i < ciglen; ++i, ++readpos )
							pendinginserts.push_back(std::make_pair(bases[readpos],qual[readpos]));
						break;
					}
					case libmaus2::bambam::BamFlagBase::LIBMAUS2_BAMBAM_CDEL:
						// handle pending inserts
						if ( pendinginserts.size() )
						{
							M[refpos].I.push_back(pendinginserts);
							pendinginserts.resize(0);
						}

						// deleting bases from the reference
						for ( uint64_t i = 0; i < ciglen; ++i, ++refpos )
							M[refpos].V.push_back(std::make_pair(padsym,0));
						break;
					case libmaus2::bambam::BamFlagBase::LIBMAUS2_BAMBAM_CREF_SKIP:
						// handle pending inserts
						if ( pendinginserts.size() )
						{
							M[refpos].I.push_back(pendinginserts);
							pendinginserts.resize(0);
						}

						// skip bases on reference
						for ( uint64_t i = 0; i < ciglen; ++i )
						{
							refpos++;
						}
						break;
					case libmaus2::bambam::BamFlagBase::LIBMAUS2_BAMBAM_CSOFT_CLIP:
						// skip bases on read
						for ( uint64_t i = 0; i < ciglen; ++i )
						{
							readpos++;
						}
						break;
					case libmaus2::bambam::BamFlagBase::LIBMAUS2_BAMBAM_CHARD_CLIP:
						break;
					case libmaus2::bambam::BamFlagBase::LIBMAUS2_BAMBAM_CPAD:
					{
						for ( uint64_t i = 0; i < ciglen; ++i, ++readpos )
							pendinginserts.push_back(std::make_pair(padsym,0));
						break;
					}
				}
			}

			if ( pendinginserts.size() )
			{
				M[refpos].I.push_back(pendinginserts);
				M[refpos].iadd++;
				pendinginserts.resize(0);
			}

			assert ( readpos == seqlen );
		}

		if ( verbose && ((++alcnt % (1024*1024)) == 0) )
			std::cerr << "[V] " << alcnt << std::endl;
	}

	while ( M.size() )
	{
		HeapEntry & H = M.begin()->second;

		if ( outputprefix.size() && (streamRefId != prevrefid) )
		{
			if ( Pstream )
			{
				std::ostringstream fnostr;
				fnostr << outputprefix << "_" << header.getRefIDName(streamRefId);
				libmaus2::aio::OutputStreamInstance PFOS(fnostr.str());
				PFOS << ">" << header.getRefIDName(streamRefId) << '\n';
				PFOS << Pstream->str() << '\n';

				Pstream.reset();
			}

			stream_ptr_type Tstream(new std::ostringstream);
			Pstream = Tstream;
			streamRefId = prevrefid;
		}

		if ( Pindex && (loadedRefId != prevrefid) )
		{
			refseqbases = Pindex->readSequence(*PCIS, Pindex->getSequenceIdByName(refidname));
			loadedRefId = prevrefid;

			if ( Mconsacc.find(loadedRefId) == Mconsacc.end() )
				Mconsacc[loadedRefId] = ConsensusAccuracy(refseqbases.size());

			consacc = &(Mconsacc[loadedRefId]);
		}

		H.toStream(std::cout,M.begin()->first,refidname,(M.begin()->first < refseqbases.size()) ? static_cast<int>(refseqbases[M.begin()->first]) : -1,Caux,consacc,Pstream.get());

		M.erase(M.begin());
	}

	if ( Pstream )
	{
		std::ostringstream fnostr;
		fnostr << outputprefix << "_" << header.getRefIDName(streamRefId);
		libmaus2::aio::OutputStreamInstance PFOS(fnostr.str());
		PFOS << ">" << header.getRefIDName(streamRefId) << '\n';
		PFOS << Pstream->str() << '\n';

		Pstream.reset();
	}

	ConsensusAccuracy constotal;
	for ( std::map<uint64_t,ConsensusAccuracy>::const_iterator ita = Mconsacc.begin(); ita != Mconsacc.end(); ++ita )
	{
		std::cerr << header.getRefIDName(ita->first) << "\t" << ita->second << std::endl;

		std::map<uint64_t,uint64_t> const M = ita->second.depthhistogram.get();
		uint64_t total = 0;
		uint64_t preavg = 0;
		for ( std::map<uint64_t,uint64_t>::const_iterator aita = M.begin(); aita != M.end(); ++aita )
		{
			total += aita->second;
			preavg += aita->first * aita->second;
		}

		uint64_t acc = 0;
		for ( std::map<uint64_t,uint64_t>::const_iterator aita = M.begin(); aita != M.end(); ++aita )
		{
			acc += aita->second;
			std::cerr << "H[" << header.getRefIDName(ita->first) << "," << aita->first << ",+]"
				<< "\t" << aita->second << "\t" << static_cast<double>(aita->second)/total
				<< "\t" << acc << "\t" << static_cast<double>(acc)/total << std::endl;
		}
		acc = 0;
		for ( std::map<uint64_t,uint64_t>::const_reverse_iterator aita = M.rbegin(); aita != M.rend(); ++aita )
		{
			acc += aita->second;
			std::cerr << "H[" << header.getRefIDName(ita->first) << "," << aita->first << ",-]"
				<< "\t" << aita->second << "\t" << static_cast<double>(aita->second)/total
				<< "\t" << acc << "\t" << static_cast<double>(acc)/total << std::endl;
		}

		std::cerr << "H[" << header.getRefIDName(ita->first) << ",avg]\t" <<
			static_cast<double>(preavg)/total << std::endl;

		constotal += ita->second;
	}
	if ( Mconsacc.size() )
	{
		std::cerr << "all\t" << constotal << std::endl;

		std::map<uint64_t,uint64_t> const M = constotal.depthhistogram.get();
		uint64_t total = 0;
		uint64_t preavg = 0;
		for ( std::map<uint64_t,uint64_t>::const_iterator aita = M.begin(); aita != M.end(); ++aita )
		{
			total += aita->second;
			preavg += aita->first * aita->second;
		}

		uint64_t acc = 0;
		for ( std::map<uint64_t,uint64_t>::const_iterator aita = M.begin(); aita != M.end(); ++aita )
		{
			acc += aita->second;
			std::cerr << "H[" << "all" << "," << aita->first << ",+]"
				<< "\t" << aita->second << "\t" << static_cast<double>(aita->second)/total
				<< "\t" << acc << "\t" << static_cast<double>(acc)/total << std::endl;
		}
		acc = 0;
		for ( std::map<uint64_t,uint64_t>::const_reverse_iterator aita = M.rbegin(); aita != M.rend(); ++aita )
		{
			acc += aita->second;
			std::cerr << "H[" << "all" << "," << aita->first << ",-]"
				<< "\t" << aita->second << "\t" << static_cast<double>(aita->second)/total
				<< "\t" << acc << "\t" << static_cast<double>(acc)/total << std::endl;
		}

		std::cerr << "H[all,avg]\t" << static_cast<double>(preavg) / total << std::endl;

	}

	return EXIT_SUCCESS;
}