score_t Phaser::aligner(size_t i_ini,
size_t j_ini,
size_t k_ini,
size_t i_end,
size_t j_end,
size_t k_end) {
size_t i_med, j_med, k_med;
score_t ans = partial_aligner(i_ini,
j_ini,
k_ini,
i_end,
j_end,
k_end,
&i_med,
&j_med,
&k_med);
if (verbose) {
printf("%lu, %lu, %lu \n", k_ini, k_med, k_end);
}
bool p1 = false;
bool p2 = false;
score_t ans_1, ans_2;
if (k_ini <= k_med && k_med != k_end &&
k_ini < k_med+1) {
ans_1= aligner(i_ini,
j_ini,
k_ini,
i_med,
j_med,
k_med);
p1 = true;
}
if (k_med+1 <= k_end &&
k_ini < k_med+1) {
ans_2= aligner(i_med + 1,
j_med + 1,
k_med + 1,
i_end ,
j_end,
k_end);
p2 = true;
}
if (p1 && p2) {
if (ans != ans_1 + ans_2) {
fprintf(stderr, "This should never happen.\n");
fprintf(stderr, "Inconsistency in recursive call, Phaser::aligner.\n");
fprintf(stderr, "Please send us a report.\n");
exit(-1);
}
} else {
if (p1 || p2) {
printf("im curious:\n");
printf("%lu, %lu, %lu \n", k_ini, k_med, k_end);
}
}
return ans;
}
void RemapKmers(const Sequence& old_s, const Sequence& new_s) {
VERIFY(this->IsAttached());
size_t old_length = old_s.size() - k_ + 1;
size_t new_length = new_s.size() - k_ + 1;
UniformPositionAligner aligner(old_s.size() - k_ + 1,
new_s.size() - k_ + 1);
Kmer old_kmer = old_s.start<Kmer>(k_);
for (size_t i = k_ - 1; i < old_s.size(); ++i) {
// Instead of shifting right
if (i != k_ - 1) {
old_kmer <<= old_s[i];
}
size_t old_kmer_offset = i - k_ + 1;
size_t new_kmer_offest = aligner.GetPosition(old_kmer_offset);
if(old_kmer_offset * 2 + 1 == old_length && new_length % 2 == 0) {
Kmer middle(unsigned(k_ - 1), new_s, new_length / 2);
if(typename Kmer::less2()(middle, !middle)) {
new_kmer_offest = new_length - 1 - new_kmer_offest;
}
}
Kmer new_kmer(unsigned(k_), new_s, new_kmer_offest);
auto it = mapping_.find(new_kmer);
if (it != mapping_.end()) {
if(verification_on_)
VERIFY(Substitute(new_kmer) == old_kmer);
mapping_.erase(it);
}
if(old_kmer != new_kmer)
mapping_[old_kmer] = new_kmer;
}
}
bool CheckCanRemap(const Sequence& old_s, const Sequence& new_s) const {
if(!CheckAllDifferent(old_s, new_s))
return false;
size_t old_length = old_s.size() - k_ + 1;
size_t new_length = new_s.size() - k_ + 1;
UniformPositionAligner aligner(old_s.size() - k_ + 1,
new_s.size() - k_ + 1);
Kmer old_kmer = old_s.start<Kmer>(k_);
old_kmer >>= 0;
for (size_t i = k_ - 1; i < old_s.size(); ++i) {
old_kmer <<= old_s[i];
size_t old_kmer_offset = i - k_ + 1;
size_t new_kmer_offest = aligner.GetPosition(old_kmer_offset);
if(old_kmer_offset * 2 + 1 == old_length && new_length % 2 == 0) {
Kmer middle(k_ - 1, new_s, new_length / 2);
if (typename Kmer::less2()(middle, !middle)) {
new_kmer_offest = new_length - 1 - new_kmer_offest;
}
}
Kmer new_kmer(k_, new_s, new_kmer_offest);
auto it = mapping_.find(new_kmer);
if (it != mapping_.end()) {
if (Substitute(new_kmer) != old_kmer) {
return false;
}
}
}
return true;
}
void KoTextEditor::setHorizontalTextAlignment(Qt::Alignment align)
{
if (isEditProtected()) {
return;
}
class Aligner : public BlockFormatVisitor
{
public:
Aligner(Qt::Alignment align) : alignment(align) {}
void visit(QTextBlock &block) const {
QTextBlockFormat format = block.blockFormat();
format.setAlignment(alignment);
QTextCursor cursor(block);
cursor.setBlockFormat(format);
}
Qt::Alignment alignment;
};
Aligner aligner(align);
d->updateState(KoTextEditor::Private::Format, i18nc("(qtundo-format)", "Change Alignment"));
BlockFormatVisitor::visitSelection(this, aligner, i18nc("(qtundo-format)", "Change Alignment"));
d->updateState(KoTextEditor::Private::NoOp);
emit textFormatChanged();
}
int main(int argc, char *argv[]) {
ArgumentParser ap;
ap.parse_args(argc, argv);
initialize_dash_dirs();
if (ap.index) {
if (ap.genome == "" || ap.seed_len <= 0 || ap.threads <= 0) {
parse_error(ap);
} else {
indexer(ap.genome, ap.seed_len, ap.threads);
}
}
else if (ap.align) {
if (ap.out == "" || ap.in == "" || ap.threads <= 0 || ap.edit_dist < 0 \
|| ap.conf <= 0 ) {
parse_error(ap);
} else {
aligner(ap.in, ap.out, ap.threads, ap.edit_dist, ap.conf);
}
}
else {
parse_error(ap);
}
return 0;
}
IAligner * CAlignTest::CreateAligner( EAlignmentAlgo algo, CScoreTbl * scoreTbl ) const
{
auto_ptr<IAligner> aligner( NewAligner( algo, m_xDropoff ) );
ASSERT( aligner.get() );
aligner->SetScoreTbl( *scoreTbl );
return aligner.release();
}
// Serilize Constructor
MessageTaskDeliver()
: Message( PROTOCOL_VERSION , 131 , 0 )
{
task_id( "" );
uri_list( );
aligner( "" );
sorter( "" );
reference( "" );
}
static
AlignmentResult<score_t>
testAlign(
const std::string& seq,
const std::string& ref)
{
AlignmentScores<score_t> scores(2,-4,-5,-1,-4);
GlobalAligner<score_t> aligner(scores);
AlignmentResult<score_t> result;
aligner.align(seq.begin(),seq.end(),ref.begin(),ref.end(),result);
return result;
}
TEST(Alignment, OutputTest) {
//TODO why do we have 3 softclipped nucleotides on both edges?
const std::string query = "PROTEINPROTEIN";
const std::string ref = "PROTEINPROTEIN";
StripedSmithWaterman::Aligner aligner(10, -10, 11, 11); //match, mismatch, gap open, gap extend
StripedSmithWaterman::Filter filter;
StripedSmithWaterman::Alignment alignment;
aligner.SetReferenceSequence(ref.c_str(), ref.length());
aligner.Align(query.c_str(), filter, &alignment);
OutputFormatter::print_alignment(std::cout, alignment, ref, query, "Ref", "Query");
}
static
JumpAlignmentResult<score_t>
testAlignScores(
const std::string& seq,
const std::string& ref1,
const std::string& ref2,
int match, int mismatch, int open, int extend, int spliceOpen, int offEdge, int spliceOffEdge)
{
AlignmentScores<score_t> scores(match, mismatch, open, extend, offEdge);
score_t jumpScore(-3);
GlobalJumpIntronAligner<score_t> aligner(scores,jumpScore,spliceOpen,spliceOffEdge);
JumpAlignmentResult<score_t> result;
aligner.align(
seq.begin(),seq.end(),
ref1.begin(),ref1.end(),
ref2.begin(),ref2.end(),
false, false, //todo make tests for all ortientations
result);
return result;
}
static
JumpAlignmentResult<score_t>
testAlignScores(
const std::string& seq,
const std::string& ref1,
const std::string& ref2,
int match, int mismatch, int open, int extend, int spliceOpen, int offEdge, int spliceOffEdge, int jump,
bool stranded, bool bp1Fw, bool bp2Fw)
{
AlignmentScores<score_t> scores(match, mismatch, open, extend, offEdge);
score_t jumpScore(jump);
GlobalJumpIntronAligner<score_t> aligner(scores,jumpScore,spliceOpen,spliceOffEdge);
JumpAlignmentResult<score_t> result;
aligner.align(
seq.begin(),seq.end(),
ref1.begin(),ref1.end(),
ref2.begin(),ref2.end(),
bp1Fw, bp2Fw, stranded,
result);
return result;
}
score_t Phaser::similarity_and_phase() {
score_t ans = aligner(0, 0, 0, M_len-1, F_len-1, C_len-1);
PrintPhaseString();
return ans;
}
Config::Config(int argc, const char **argv)
{
Command_line_parser parser;
parser.add_command("makedb", "Build DIAMOND database from a FASTA file")
.add_command("blastp", "Align amino acid query sequences against a protein reference database")
.add_command("blastx", "Align DNA query sequences against a protein reference database")
.add_command("view", "View DIAMOND alignment archive (DAA) formatted file")
.add_command("help", "Produce help message")
.add_command("version", "Display version information")
.add_command("getseq", "")
.add_command("benchmark", "")
.add_command("random-seqs", "")
.add_command("compare", "");
Options_group general ("General options");
general.add()
("threads", 'p', "number of CPU threads", threads_)
("db", 'd', "database file", database)
("out", 'o', "output file", output_file)
("outfmt", 'f', "output format (tab/sam/xml/daa)", output_format)
("verbose", 'v', "verbose console output", verbose)
("log", 0, "enable debug log", debug_log)
("quiet", 0, "disable console output", quiet);
Options_group makedb("Makedb options");
makedb.add()
("in", 0, "input reference file in FASTA format", input_ref_file)
#ifdef EXTRA
("dbtype", po::value<string>(&program_options::db_type), "database type (nucl/prot)")
#endif
;
Options_group aligner("Aligner options");
aligner.add()
("query",'q', "input query file", query_file)
("max-target-seqs",'k', "maximum number of target sequences to report alignments for", max_alignments, uint64_t(25))
("top", 0, "report alignments within this percentage range of top alignment score (overrides --max-target-seqs)", toppercent, 100.0)
("compress", 0, "compression for output files (0=none, 1=gzip)", compression)
("evalue",'e', "maximum e-value to report alignments", max_evalue, 0.001)
("min-score", 0, "minimum bit score to report alignments (overrides e-value setting)", min_bit_score)
("id", 0, "minimum identity% to report an alignment", min_id)
("query-cover", 0, "minimum query cover% to report an alignment", query_cover)
("sensitive", 0, "enable sensitive mode (default: fast)", mode_sensitive)
("more-sensitive", 0, "enable more sensitive mode (default: fast)", mode_more_sensitive)
("block-size", 'b', "sequence block size in billions of letters (default=2.0)", chunk_size, 2.0)
("index-chunks",'c', "number of chunks for index processing", lowmem, 4u)
("tmpdir",'t', "directory for temporary files", tmpdir)
("gapopen", 0, "gap open penalty (default=11 for protein)", gap_open, -1)
("gapextend", 0, "gap extension penalty (default=1 for protein)", gap_extend, -1)
#ifdef EXTRA
("reward", po::value<int>(&program_options::reward)->default_value(2), "match reward score (blastn only)")
("penalty", po::value<int>(&program_options::penalty)->default_value(-3), "mismatch penalty score (blastn only)")
#endif
("matrix", 0, "score matrix for protein alignment", matrix, string("blosum62"))
("seg", 0, "enable SEG masking of queries (yes/no)", seg)
("salltitles", 0, "print full subject titles in output files", salltitles);
Options_group advanced("Advanced options");
advanced.add()
("run-len", 'l', "mask runs between stop codons shorter than this length", run_len)
("freq-sd", 0, "number of standard deviations for ignoring frequent seeds", freq_sd, 0.0)
("id2", 0, "minimum number of identities for stage 1 hit", min_identities)
("window", 'w', "window size for local hit search", window)
("xdrop", 'x', "xdrop for ungapped alignment", xdrop, 20)
("gapped-xdrop", 'X', "xdrop for gapped alignment in bits", gapped_xdrop, 20)
("ungapped-score", 0, "minimum raw alignment score to continue local extension", min_ungapped_raw_score)
("hit-band", 0, "band for hit verification", hit_band)
("hit-score", 0, "minimum score to keep a tentative alignment", min_hit_score)
("band", 0, "band for dynamic programming computation", padding)
("shapes", 's', "number of seed shapes (0 = all available)", shapes)
("index-mode", 0, "index mode (0=4x12, 1=16x9)", index_mode)
("fetch-size", 0, "trace point fetch size", fetch_size, 4096u)
("rank-factor", 0, "include subjects within this range of max-target-seqs", rank_factor, 2.0)
("rank-ratio", 0, "include subjects within this ratio of last hit", rank_ratio, 0.35)
("single-domain", 0, "Discard secondary domains within one target sequence", single_domain)
("dbsize", 0, "effective database size (in letters)", db_size)
("no-auto-append", 0, "disable auto appending of DAA and DMND file extensions", no_auto_append)
("target-fetch-size", 0, "", target_fetch_size, 4u);
Options_group view_options("View options");
view_options.add()
("daa", 'a', "DIAMOND alignment archive (DAA) file", daa_file)
("forwardonly", 0, "only show alignments of forward strand", forwardonly);
Options_group hidden_options("");
hidden_options.add()
("extend-all", 0, "extend all seed hits", extend_all)
("local-align", 0, "Local alignment algorithm", local_align_mode, 0u)
("slow-search", 0, "", slow_search)
("seq", 0, "", seq_no)
("ht", 0, "", ht_mode)
("old-freq", 0, "", old_freq)
("qp", 0, "", query_parallel)
("match1", 0, "", match_file1)
("match2", 0, "", match_file2)
("max-hits", 'C', "maximum number of hits to consider for one seed", hit_cap)
("seed-freq", 0, "maximum seed frequency", max_seed_freq, -15.0);
parser.add(general).add(makedb).add(aligner).add(advanced).add(view_options).add(hidden_options);
parser.store(argc, argv, command);
switch (command) {
case Config::makedb:
if (input_ref_file == "")
throw std::runtime_error("Missing parameter: input file (--in)");
if (database == "")
throw std::runtime_error("Missing parameter: database file (--db/-d)");
if (chunk_size != 2.0)
throw std::runtime_error("Invalid option: --block-size/-b. Block size is set for the alignment commands.");
break;
case Config::blastp:
case Config::blastx:
if (query_file == "")
throw std::runtime_error("Missing parameter: query file (--query/-q)");
if (database == "")
throw std::runtime_error("Missing parameter: database file (--db/-d)");
if (daa_file.length() > 0) {
if (output_file.length() > 0)
throw std::runtime_error("Options --daa and --out cannot be used together.");
if (output_format.length() > 0 && output_format != "daa")
throw std::runtime_error("Invalid parameter: --daa/-a. Output file is specified with the --out/-o parameter.");
output_file = daa_file;
}
if (daa_file.length() > 0 || output_format == "daa") {
if (compression != 0)
throw std::runtime_error("Compression is not supported for DAA format.");
if (!no_auto_append)
auto_append_extension(output_file, ".daa");
}
break;
case Config::view:
if (daa_file == "")
throw std::runtime_error("Missing parameter: DAA file (--daa/-a)");
default:
;
}
if (hit_cap != 0)
throw std::runtime_error("Deprecated parameter: --max-hits/-C.");
if (debug_log)
verbosity = 3;
else if (quiet)
verbosity = 0;
else if (verbose)
verbosity = 2;
else if (((command == Config::view || command == blastx || command == blastp) && output_file == "")
|| command == Config::version)
verbosity = 0;
else
verbosity = 1;
switch (verbosity) {
case 0:
message_stream = Message_stream(false);
break;
case 3:
log_stream = Message_stream();
case 2:
verbose_stream = Message_stream();
default:
;
}
if (!no_auto_append) {
auto_append_extension(database, ".dmnd");
if (command == Config::view)
auto_append_extension(daa_file, ".daa");
if (compression == 1)
auto_append_extension(output_file, ".gz");
}
message_stream << Const::program_name << " v" << Const::version_string << "." << (unsigned)Const::build_version << " | by Benjamin Buchfink <*****@*****.**>" << endl;
message_stream << "Check http://github.com/bbuchfink/diamond for updates." << endl << endl;
#ifndef NDEBUG
verbose_stream << "Assertions enabled." << endl;
#endif
set_option(threads_, tthread::thread::hardware_concurrency());
switch (command) {
case Config::makedb:
case Config::blastp:
case Config::blastx:
case Config::view:
message_stream << "#CPU threads: " << threads_ << endl;
default:
;
}
if (command == Config::blastp || command == Config::blastx || command == Config::benchmark) {
if (tmpdir == "")
tmpdir = extract_dir(output_file);
if (gap_open == -1)
gap_open = 11;
if (gap_extend == -1)
gap_extend = 1;
score_matrix = Score_matrix(to_upper_case(matrix), gap_open, gap_extend, reward, penalty);
message_stream << "Scoring parameters: " << score_matrix << endl;
if (seg == "" && command == blastx)
seg = "yes";
verbose_stream << "SEG masking = " << (seg == "yes") << endl;
have_ssse3 = check_SSSE3();
if (have_ssse3)
verbose_stream << "SSSE3 enabled." << endl;
verbose_stream << "Reduction: " << Reduction::reduction << endl;
if (mode_more_sensitive) {
set_option(index_mode, 1u);
set_option(freq_sd, 200.0);
}
else if (mode_sensitive) {
set_option(index_mode, 1u);
set_option(freq_sd, 10.0);
}
else {
set_option(index_mode, 0u);
set_option(freq_sd, 50.0);
}
verbose_stream << "Seed frequency SD: " << freq_sd << endl;
::shapes = shape_config(index_mode, shapes);
verbose_stream << "Shape configuration: " << ::shapes << endl;
message_stream << "#Target sequences to report alignments for: ";
if (max_alignments == 0) {
max_alignments = std::numeric_limits<uint64_t>::max();
message_stream << "unlimited" << endl;
} else
message_stream << max_alignments << endl;
}
if (command == blastx)
input_value_traits = nucleotide_traits;
if (command == help)
parser.print_help();
/*log_stream << "sizeof(hit)=" << sizeof(hit) << " sizeof(packed_uint40_t)=" << sizeof(packed_uint40_t)
<< " sizeof(sorted_list::entry)=" << sizeof(sorted_list::entry) << endl;*/
}
/*
* allocation d'une zone memoire
* appel a la fonction mem_fit_first
* prend en parametre la taille de la zone a allouer
* renvoie l'adresse de la zone allouee (ou l'utilisateur peut ecrire)
* ou NULL si allocation impossible (size = 0 ou manque de place)
*/
void* mem_alloc(size_t size)
{
prendre_verrou();
struct fb bloc_choisi;
size_t* ptr_taille;
struct fb* ptr_bloc_choisi;
size_t taille_requise = size + sizeof(size_t);
size_t taille_restante;
struct fb* ptr_new_bloc;
void* retour = NULL;
// allocation d'au moins 1 octet
if (size > 0)
{
// appel a la methode mem_fit_first afin de recuperer l'adresse du bloc choisi
ptr_bloc_choisi = f_mem_fit(size);
// allocation impossible, pas de bloc libre de taille suffisante : renvoie NULL
if(ptr_bloc_choisi == NULL)
{
relacher_verrou();
return NULL;
}
// on copie les donnees de la structure du bloc libre dans la structure bloc_choisi
// avant ecriture de la taille a la place
bloc_choisi = *ptr_bloc_choisi;
// ajout du padding si besoin
taille_requise = aligner(taille_requise);
// on stocke la taille totale du bloc au debut
ptr_taille = (size_t*) ptr_bloc_choisi;
*ptr_taille = taille_requise;
// on calcule la taille restante du bloc choisi
taille_restante = bloc_choisi.size - taille_requise;
// si on a encore de la place pour au moins la structure fb : on peut creer un nouveau bloc libre
if ( taille_restante >= sizeof(struct fb) )
{
// ecriture de la structure du nouveau bloc
ptr_new_bloc = (struct fb*)((char*)ptr_bloc_choisi + taille_requise);
(*ptr_new_bloc).size = taille_restante;
(*ptr_new_bloc).next = bloc_choisi.next;
}
else
{
// pas de nouveau bloc libre, le suivant sera le suivant de l'ancien bloc
ptr_new_bloc = bloc_choisi.next;
// ajout de la taille en plus dans le bloc alloue (padding)
ptr_bloc_choisi->size += taille_restante;
}
// appel a la fonction maj_chainage
maj_chainage_alloc(ptr_bloc_choisi, ptr_new_bloc);
// renvoie l'adresse decalee de sizeof(size_t) representant la taille de la zone allouee
retour = (void*)((char*)ptr_bloc_choisi + sizeof(size_t));
relacher_verrou();
}
else if(size == 0)
{
relacher_verrou();
// si demande d'allocation de 0 octet, on alloue 1 octet
retour = mem_alloc(1);
}
else
{
// allocation impossible : la taille doit etre > 0
retour = NULL;
relacher_verrou();
}
return retour;
}
void CAppNWA::x_RunOnPair() const
{
const CArgs& args = GetArgs();
// analyze parameters
const bool bMM = args["mm"];
const bool bMT = args["mt"];
bool output_type1 ( args["o1"] );
bool output_type2 ( args["o2"] );
bool output_asn ( args["oasn"] );
bool output_fasta ( args["ofasta"] );
int band (args["band"].AsInteger());
int shift(args["shift"].AsInteger());
if(bMT && !bMM) {
NCBI_THROW(CAppNWAException,
eInconsistentParameters,
"Mutliple thread mode supported "
"for Myers-Miller method only (invoke with -mm)");
}
if(bMM && band >= 0) {
NCBI_THROW(CAppNWAException,
eInconsistentParameters,
"-mm and -band are inconsistent with each other");
}
#ifndef NCBI_THREADS
if(bMT) {
NCBI_THROW(CAppNWAException,
eNotSupported,
"This application was built without multithreading support. "
"To run in multiple threads, please re-configure and rebuild"
" with proper options.");
}
#endif
// read input sequences
vector<char> v1, v2;
CRef<CSeq_id> seqid1 = x_ReadFastaFile(args["seq1"].AsString(), &v1);
CRef<CSeq_id> seqid2 = x_ReadFastaFile(args["seq2"].AsString(), &v2);
// determine sequence/score matrix type
const SNCBIPackedScoreMatrix* psm =
(args["matrix"].AsString() == "blosum62")? &NCBISM_Blosum62: 0;
CNWAligner* pnwaligner = 0;
if(bMM) {
pnwaligner = new CMMAligner(&v1[0], v1.size(), &v2[0], v2.size(), psm);
}
else if (band < 0) {
pnwaligner = new CNWAligner(&v1[0], v1.size(), &v2[0], v2.size(), psm);
}
else {
CBandAligner * ba = new CBandAligner(&v1[0], v1.size(), &v2[0], v2.size(),
psm, band);
Uint1 where = shift >= 0? 0: 1;
ba->SetShift(where,abs(shift));
pnwaligner = ba;
}
auto_ptr<CNWAligner> aligner (pnwaligner);
if(psm == NULL) {
aligner->SetWm (args["Wm"]. AsInteger());
aligner->SetWms (args["Wms"].AsInteger());
aligner->SetScoreMatrix(NULL);
}
aligner->SetWg (args["Wg"]. AsInteger());
aligner->SetWs (args["Ws"]. AsInteger());
aligner->SetScoreMatrix(psm); // re-set score matrix to handle
// possible ambiguity chars
if(bMT && bMM) {
CMMAligner* pmma = static_cast<CMMAligner*> (aligner.get());
pmma -> EnableMultipleThreads();
}
auto_ptr<ofstream> pofs1 (0);
auto_ptr<ofstream> pofs2 (0);
auto_ptr<ofstream> pofsAsn (0);
auto_ptr<ofstream> pofsFastA (0);
if(output_type1) {
pofs1.reset(open_ofstream (args["o1"].AsString()).release());
}
if(output_type2) {
pofs2.reset(open_ofstream (args["o2"].AsString()).release());
}
if(output_asn) {
pofsAsn.reset(open_ofstream (args["oasn"].AsString()).release());
}
if(output_fasta) {
pofsFastA.reset(open_ofstream (args["ofasta"].AsString()).release());
}
{{ // setup end penalties
string ends = args["esf"].AsString();
bool L1 = ends[0] == 'z';
bool R1 = ends[1] == 'z';
bool L2 = ends[2] == 'z';
bool R2 = ends[3] == 'z';
aligner->SetEndSpaceFree(L1, R1, L2, R2);
}}
int score = aligner->Run();
cerr << "Score = " << score << endl;
CNWFormatter formatter (*aligner);
formatter.SetSeqIds(seqid1, seqid2);
const size_t line_width = 100;
string s;
if(pofs1.get()) {
formatter.AsText(&s, CNWFormatter::eFormatType1, line_width);
*pofs1 << s;
}
if(pofs2.get()) {
formatter.AsText(&s, CNWFormatter::eFormatType2, line_width);
*pofs2 << s;
}
if(pofsAsn.get()) {
formatter.AsText(&s, CNWFormatter::eFormatAsn, line_width);
*pofsAsn << s;
}
if(pofsFastA.get()) {
formatter.AsText(&s, CNWFormatter::eFormatFastA, line_width);
*pofsFastA << s;
}
if(!output_type1 && !output_type2
&& !output_asn && !output_fasta)
{
formatter.AsText(&s, CNWFormatter::eFormatType2, line_width);
cout << s;
}
}
int CAlignTest::Execute()
{
CScoreTbl scoreTbl( m_identityScore, m_mismatchScore, m_gapOpeningScore, m_gapExtentionScore );
auto_ptr<IAligner> aligner( CreateAligner( m_algo, &scoreTbl ) );
CAlignerBase::SetPrintDebug( true );
if( m_flags & fAlign_colorspace )
THROW( logic_error, "Colorspace option is not immplemented" );
if( GetArgIndex() + 2 > GetArgCount() )
THROW( runtime_error, "Two sequences are required!" );
if( m_offsetQuery < 0 || m_offsetSubject < 0 )
THROW( runtime_error, "Offset should never be negative" );
const char * qs = GetArg( GetArgIndex() );
const char * ss = GetArg( GetArgIndex() + 1 );
int ql = strlen( qs );
int sl = strlen( ss );
cerr << DISPLAY( qs ) << DISPLAY( ql ) << endl;
cerr << DISPLAY( ss ) << DISPLAY( sl ) << endl;
if( ql == 0 || sl == 0 )
THROW( runtime_error, "Sequences should have at least one base!" );
vector<char> query;
vector<char> subject;
query.reserve( ql );
subject.reserve( sl );
while( *qs ) query.push_back( CNcbi8naBase( CIupacnaBase( *qs++ ) ) );
while( *ss ) subject.push_back( CNcbi8naBase( CIupacnaBase( *ss++ ) ) );
qs = &query[0];
ss = &subject[0];
qs += m_offsetQuery; ql -= m_offsetQuery;
ss += m_offsetSubject; sl -= m_offsetSubject;
if( ql <= 0 || sl <= 0 )
THROW( runtime_error, "Offset is too big" );
if( GetFlags( fQuery_reverse ) ) { qs = qs + ql; ql = -ql; }
if( GetFlags( fSubject_reverse ) ) { ss = ss + sl; sl = -sl; }
int aflags = CAlignerBase::fComputePicture | CAlignerBase::fComputeScore | CAlignerBase::fPictureSubjectStrand;
aligner->SetBestPossibleQueryScore( min( ql, sl ) * m_identityScore );
aligner->Align( CSeqCoding::eCoding_ncbi8na, qs, ql, CSeqCoding::eCoding_ncbi8na, ss, sl, aflags );
const CAlignerBase& abase = aligner->GetAlignerBase();
cout << ( GetFlags( fQuery_reverse ) ? 3 : 5 ) << "'=" << abase.GetQueryString() << "=" << ( GetFlags( fQuery_reverse ) ? 5 : 3 ) << "'\n";
cout << " " << abase.GetAlignmentString() << " "
<< " i=" << abase.GetIdentityCount()
<< ", m=" << abase.GetMismatchCount()
<< ", g=" << abase.GetIndelCount()
<< ", s=" << abase.GetRawScore() << "/" << abase.GetBestQueryScore() << "=" << abase.GetScore() << "%\n";
cout << "5'=" << abase.GetSubjectString() << "=3'\n";
return 0;
}
int main(int argc, char **argv)
{
return aligner(argc, argv);
}
