struct bed *bigBedGetFilteredBedsOnRegions(struct sqlConnection *conn,
char *db, char *table, struct region *regionList, struct lm *lm,
int *retFieldCount)
/* Get list of beds from bigBed, in all regions, that pass filtering. */
{
/* Connect to big bed and get metadata and filter. */
char *fileName = bigBedFileName(table, conn);
struct bbiFile *bbi = bigBedFileOpen(fileName);
struct asObject *as = bigBedAsOrDefault(bbi);
struct asFilter *filter = asFilterFromCart(cart, db, table, as);
/* Get beds a region at a time. */
struct bed *bedList = NULL;
struct region *region;
for (region = regionList; region != NULL; region = region->next)
addFilteredBedsOnRegion(bbi, region, table, filter, lm, &bedList);
slReverse(&bedList);
/* Clean up and return. */
if (retFieldCount != NULL)
*retFieldCount = bbi->definedFieldCount;
bbiFileClose(&bbi);
freeMem(fileName);
return bedList;
}
struct bed *bamGetFilteredBedsOnRegions(struct sqlConnection *conn,
char *db, char *table, struct region *regionList, struct lm *lm,
int *retFieldCount)
/* Get list of beds from BAM, in all regions, that pass filtering. */
{
int maxOut = bigFileMaxOutput();
/* Figure out bam file name get column info and filter. */
struct asObject *as = bamAsObj();
struct asFilter *filter = asFilterFromCart(cart, db, table, as);
struct hash *idHash = identifierHash(db, table);
/* Get beds a region at a time. */
struct bed *bedList = NULL;
struct region *region;
for (region = regionList; region != NULL; region = region->next)
{
char *fileName = bamFileName(table, conn, region->chrom);
addFilteredBedsOnRegion(fileName, region, table, filter, lm, &bedList, idHash, &maxOut);
freeMem(fileName);
if (maxOut <= 0)
{
warn("Reached output limit of %d data values, please make region smaller,\n"
"\tor set a higher output line limit with the filter settings.", bigFileMaxOutput());
break;
}
}
slReverse(&bedList);
return bedList;
}
void bigBedTabOut(char *db, char *table, struct sqlConnection *conn, char *fields, FILE *f)
/* Print out selected fields from Big Bed. If fields is NULL, then print out all fields. */
{
if (f == NULL)
f = stdout;
/* Convert comma separated list of fields to array. */
int fieldCount = chopByChar(fields, ',', NULL, 0);
char **fieldArray;
AllocArray(fieldArray, fieldCount);
chopByChar(fields, ',', fieldArray, fieldCount);
/* Get list of all fields in big bed and turn it into a hash of column indexes keyed by
* column name. */
struct hash *fieldHash = hashNew(0);
struct slName *bb, *bbList = bigBedGetFields(table, conn);
int i;
for (bb = bbList, i=0; bb != NULL; bb = bb->next, ++i)
hashAddInt(fieldHash, bb->name, i);
// If bigBed has name column, look up pasted/uploaded identifiers if any:
struct hash *idHash = NULL;
if (slCount(bbList) >= 4)
idHash = identifierHash(db, table);
/* Create an array of column indexes corresponding to the selected field list. */
int *columnArray;
AllocArray(columnArray, fieldCount);
for (i=0; i<fieldCount; ++i)
{
columnArray[i] = hashIntVal(fieldHash, fieldArray[i]);
}
/* Output row of labels */
fprintf(f, "#%s", fieldArray[0]);
for (i=1; i<fieldCount; ++i)
fprintf(f, "\t%s", fieldArray[i]);
fprintf(f, "\n");
/* Open up bigBed file. */
char *fileName = bigBedFileName(table, conn);
struct bbiFile *bbi = bigBedFileOpen(fileName);
struct asObject *as = bigBedAsOrDefault(bbi);
struct asFilter *filter = NULL;
if (anyFilter())
{
filter = asFilterFromCart(cart, db, table, as);
if (filter)
{
fprintf(f, "# Filtering on %d columns\n", slCount(filter->columnList));
}
}
/* Loop through outputting each region */
struct region *region, *regionList = getRegions();
for (region = regionList; region != NULL; region = region->next)
{
struct lm *lm = lmInit(0);
struct bigBedInterval *iv, *ivList = bigBedIntervalQuery(bbi, region->chrom,
region->start, region->end, 0, lm);
char *row[bbi->fieldCount];
char startBuf[16], endBuf[16];
for (iv = ivList; iv != NULL; iv = iv->next)
{
bigBedIntervalToRow(iv, region->chrom, startBuf, endBuf, row, bbi->fieldCount);
if (asFilterOnRow(filter, row))
{
if ((idHash != NULL) && (hashLookup(idHash, row[3]) == NULL))
continue;
int i;
fprintf(f, "%s", row[columnArray[0]]);
for (i=1; i<fieldCount; ++i)
fprintf(f, "\t%s", row[columnArray[i]]);
fprintf(f, "\n");
}
}
lmCleanup(&lm);
}
/* Clean up and exit. */
bbiFileClose(&bbi);
hashFree(&fieldHash);
freeMem(fieldArray);
freeMem(columnArray);
}
void bamTabOut(char *db, char *table, struct sqlConnection *conn, char *fields, FILE *f)
/* Print out selected fields from BAM. If fields is NULL, then print out all fields. */
{
struct hTableInfo *hti = NULL;
hti = getHti(db, table, conn);
struct hash *idHash = NULL;
char *idField = getIdField(db, curTrack, table, hti);
int idFieldNum = 0;
/* if we know what field to use for the identifiers, get the hash of names */
if (idField != NULL)
idHash = identifierHash(db, table);
if (f == NULL)
f = stdout;
/* Convert comma separated list of fields to array. */
int fieldCount = chopByChar(fields, ',', NULL, 0);
char **fieldArray;
AllocArray(fieldArray, fieldCount);
chopByChar(fields, ',', fieldArray, fieldCount);
/* Get list of all fields in big bed and turn it into a hash of column indexes keyed by
* column name. */
struct hash *fieldHash = hashNew(0);
struct slName *bb, *bbList = bamGetFields();
int i;
for (bb = bbList, i=0; bb != NULL; bb = bb->next, ++i)
{
/* if we know the field for identifiers, save it away */
if ((idField != NULL) && sameString(idField, bb->name))
idFieldNum = i;
hashAddInt(fieldHash, bb->name, i);
}
/* Create an array of column indexes corresponding to the selected field list. */
int *columnArray;
AllocArray(columnArray, fieldCount);
for (i=0; i<fieldCount; ++i)
{
columnArray[i] = hashIntVal(fieldHash, fieldArray[i]);
}
/* Output row of labels */
fprintf(f, "#%s", fieldArray[0]);
for (i=1; i<fieldCount; ++i)
fprintf(f, "\t%s", fieldArray[i]);
fprintf(f, "\n");
struct asObject *as = bamAsObj();
struct asFilter *filter = NULL;
if (anyFilter())
{
filter = asFilterFromCart(cart, db, table, as);
if (filter)
{
fprintf(f, "# Filtering on %d columns\n", slCount(filter->columnList));
}
}
/* Loop through outputting each region */
struct region *region, *regionList = getRegions();
int maxOut = bigFileMaxOutput();
for (region = regionList; region != NULL && (maxOut > 0); region = region->next)
{
struct lm *lm = lmInit(0);
char *fileName = bamFileName(table, conn, region->chrom);
struct samAlignment *sam, *samList = bamFetchSamAlignment(fileName, region->chrom,
region->start, region->end, lm);
char *row[SAMALIGNMENT_NUM_COLS];
char numBuf[BAM_NUM_BUF_SIZE];
for (sam = samList; sam != NULL && (maxOut > 0); sam = sam->next)
{
samAlignmentToRow(sam, numBuf, row);
if (asFilterOnRow(filter, row))
{
/* if we're looking for identifiers, check if this matches */
if ((idHash != NULL)&&(hashLookup(idHash, row[idFieldNum]) == NULL))
continue;
int i;
fprintf(f, "%s", row[columnArray[0]]);
for (i=1; i<fieldCount; ++i)
fprintf(f, "\t%s", row[columnArray[i]]);
fprintf(f, "\n");
maxOut --;
}
}
freeMem(fileName);
lmCleanup(&lm);
}
if (maxOut == 0)
warn("Reached output limit of %d data values, please make region smaller,\n\tor set a higher output line limit with the filter settings.", bigFileMaxOutput());
/* Clean up and exit. */
hashFree(&fieldHash);
freeMem(fieldArray);
freeMem(columnArray);
}
void vcfTabOut(char *db, char *table, struct sqlConnection *conn, char *fields, FILE *f,
boolean isTabix)
/* Print out selected fields from VCF. If fields is NULL, then print out all fields. */
{
struct hTableInfo *hti = NULL;
hti = getHti(db, table, conn);
struct hash *idHash = NULL;
char *idField = getIdField(db, curTrack, table, hti);
int idFieldNum = 0;
/* if we know what field to use for the identifiers, get the hash of names */
if (idField != NULL)
idHash = identifierHash(db, table);
if (f == NULL)
f = stdout;
/* Convert comma separated list of fields to array. */
int fieldCount = chopByChar(fields, ',', NULL, 0);
char **fieldArray;
AllocArray(fieldArray, fieldCount);
chopByChar(fields, ',', fieldArray, fieldCount);
/* Get list of all fields in big bed and turn it into a hash of column indexes keyed by
* column name. */
struct hash *fieldHash = hashNew(0);
struct slName *bb, *bbList = vcfGetFields();
int i;
for (bb = bbList, i=0; bb != NULL; bb = bb->next, ++i)
{
/* if we know the field for identifiers, save it away */
if ((idField != NULL) && sameString(idField, bb->name))
idFieldNum = i;
hashAddInt(fieldHash, bb->name, i);
}
/* Create an array of column indexes corresponding to the selected field list. */
int *columnArray;
AllocArray(columnArray, fieldCount);
for (i=0; i<fieldCount; ++i)
{
columnArray[i] = hashIntVal(fieldHash, fieldArray[i]);
}
// If we are outputting a subset of fields, invalidate the VCF header.
boolean allFields = (fieldCount == VCFDATALINE_NUM_COLS);
if (!allFields)
fprintf(f, "# Only selected columns are included below; output is not valid VCF.\n");
struct asObject *as = vcfAsObj();
struct asFilter *filter = NULL;
if (anyFilter())
filter = asFilterFromCart(cart, db, table, as);
/* Loop through outputting each region */
struct region *region, *regionList = getRegions();
int maxOut = bigFileMaxOutput();
struct trackDb *tdb = hashFindVal(fullTableToTdbHash, table);
// Include the header, absolutely necessary for VCF parsing.
boolean printedHeader = FALSE;
// Temporary storage for row-ification:
struct dyString *dyAlt = newDyString(1024);
struct dyString *dyFilter = newDyString(1024);
struct dyString *dyInfo = newDyString(1024);
struct dyString *dyGt = newDyString(1024);
struct vcfRecord *rec;
for (region = regionList; region != NULL && (maxOut > 0); region = region->next)
{
char *fileName = vcfFileName(tdb, conn, table, region->chrom);
struct vcfFile *vcff;
if (isTabix)
vcff = vcfTabixFileMayOpen(fileName, region->chrom, region->start, region->end,
100, maxOut);
else
vcff = vcfFileMayOpen(fileName, region->chrom, region->start, region->end,
100, maxOut, TRUE);
if (vcff == NULL)
noWarnAbort();
// If we are outputting all fields, but this VCF has no genotype info, omit the
// genotype columns from output:
if (allFields && vcff->genotypeCount == 0)
fieldCount = VCFDATALINE_NUM_COLS - 2;
if (!printedHeader)
{
fprintf(f, "%s", vcff->headerString);
if (filter)
fprintf(f, "# Filtering on %d columns\n", slCount(filter->columnList));
if (!allFields)
{
fprintf(f, "#%s", fieldArray[0]);
for (i=1; i<fieldCount; ++i)
fprintf(f, "\t%s", fieldArray[i]);
fprintf(f, "\n");
}
printedHeader = TRUE;
}
char *row[VCFDATALINE_NUM_COLS];
char numBuf[VCF_NUM_BUF_SIZE];
for (rec = vcff->records; rec != NULL && (maxOut > 0); rec = rec->next)
{
vcfRecordToRow(rec, region->chrom, numBuf, dyAlt, dyFilter, dyInfo, dyGt, row);
if (asFilterOnRow(filter, row))
{
/* if we're looking for identifiers, check if this matches */
if ((idHash != NULL) && (hashLookup(idHash, row[idFieldNum]) == NULL))
continue;
// All fields output: after asFilter'ing, preserve original VCF chrom
if (allFields && !sameString(rec->chrom, region->chrom))
row[0] = rec->chrom;
int i;
fprintf(f, "%s", row[columnArray[0]]);
for (i=1; i<fieldCount; ++i)
{
fprintf(f, "\t%s", row[columnArray[i]]);
}
fprintf(f, "\n");
maxOut --;
}
}
vcfFileFree(&vcff);
freeMem(fileName);
}
if (maxOut == 0)
warn("Reached output limit of %d data values, please make region smaller,\n\tor set a higher output line limit with the filter settings.", bigFileMaxOutput());
/* Clean up and exit. */
dyStringFree(&dyAlt); dyStringFree(&dyFilter); dyStringFree(&dyInfo); dyStringFree(&dyGt);
hashFree(&fieldHash);
freeMem(fieldArray);
freeMem(columnArray);
}
