Ejemplo n.º 1
0
int main(int argc, char *argv[])
{
  embInitPV("ggeneskew", argc, argv, "GEMBASSY", "1.0.3");

  AjPSeqall seqall;
  AjPSeq    seq;
  AjPStr    inseq = NULL;

  ajint	 window     = 0;
  ajint	 slide      = 0;
  AjBool cumulative = ajFalse;
  AjBool gc3        = ajFalse;
  AjPStr basetype   = NULL;

  AjBool accid  = ajFalse;
  AjPStr restid = NULL;
  AjPStr seqid  = NULL;

  AjPStr base = NULL;
  AjPStr url  = NULL;

  AjPFile tmpfile = NULL;
  AjPStr  tmpname = NULL;

  AjBool      plot = 0;
  AjPFile     outf = NULL;
  AjPFilebuff buff = NULL;
  AjPGraph    mult = NULL;

  gPlotParams gpp;
  AjPStr      title = NULL;

  seqall     = ajAcdGetSeqall("sequence");
  window     = ajAcdGetInt("window");
  slide      = ajAcdGetInt("slide");
  cumulative = ajAcdGetBoolean("cumulative");
  gc3        = ajAcdGetBoolean("gctri");
  basetype   = ajAcdGetSelectSingle("base");
  accid      = ajAcdGetBoolean("accid");

  plot = ajAcdGetToggle("plot");
  outf = ajAcdGetOutfile("outfile");
  mult = ajAcdGetGraphxy("graph");

  if(ajStrMatchC(base, "none"))
    basetype = ajStrNewC("");

  base = ajStrNewC("rest.g-language.org");

  gAssignUniqueName(&tmpname);

  while(ajSeqallNext(seqall, &seq))
    {
      inseq = NULL;

      if(!accid)
        {
          if(gFormatGenbank(seq, &inseq))
            {
              gAssignUniqueName(&tmpname);

              tmpfile = ajFileNewOutNameS(tmpname);

              if(!tmpfile)
                {
                  ajFmtError("Output file (%S) open error\n", tmpname);
                  embExitBad();
                }

              ajFmtPrintF(tmpfile, "%S", inseq);
              ajFileClose(&tmpfile);
              ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
              gFilePostSS(url, tmpname, &restid);
              ajStrDel(&url);
              ajSysFileUnlinkS(tmpname);
            }
          else
            {
              ajFmtError("Sequence does not have features\n"
                         "Proceeding with sequence accession ID\n");
              accid = ajTrue;
            }
        }

      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      if(ajStrGetLen(seqid) == 0)
        {
          ajStrAssignS(&seqid, ajSeqGetNameS(seq));
        }

      if(ajStrGetLen(seqid) == 0)
        {
          ajWarn("No valid header information\n");
        }

      if(accid)
        {
          ajStrAssignS(&restid, seqid);
          if(ajStrGetLen(seqid) == 0)
            {
              ajDie("Cannot proceed without header with -accid\n");
            }

          if(!gValID(seqid))
            {
              ajDie("Invalid accession ID:%S, exiting\n", seqid);
            }
        }

      url = ajStrNew();

      ajFmtPrintS(&url, "http://%S/%S/geneskew/window=%d/slide=%d/"
                  "cumulative=%d/gc3=%d/base=%S/output=f/tag=gene",
                  base, restid, window, slide, cumulative, gc3, basetype);

      if(plot)
        {
          title = ajStrNew();

          ajStrAppendC(&title, argv[0]);
          ajStrAppendC(&title, " of ");
          ajStrAppendS(&title, seqid);

          gpp.title = ajStrNewS(title);
          gpp.xlab = ajStrNewC("gene skew");
          gpp.ylab = ajStrNewC("bp");

          if(!gFilebuffURLS(url, &buff))
            {
              ajDie("File downloading error from:\n%S\n", url);
            }

          if(!gPlotFilebuff(buff, mult, &gpp))
            {
              ajDie("Error in plotting\n");
            }

          AJFREE(gpp.title);
          AJFREE(gpp.xlab);
          AJFREE(gpp.ylab);
          ajStrDel(&title);
          ajFilebuffDel(&buff);
        }
      else
        {
          ajFmtPrintF(outf, "Sequence: %S\n", seqid);
          if(!gFileOutURLS(url, &outf))
            {
              ajDie("File downloading error from:\n%S\n", url);
            }
        }

      ajStrDel(&url);
      ajStrDel(&restid);
      ajStrDel(&seqid);
      ajStrDel(&inseq);
    }

  ajFileClose(&outf);

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&base);

  embExit();

  return 0;
}
Ejemplo n.º 2
0
int main(int argc, char *argv[])
{
  embInitPV("gdeltagcskew", argc, argv, "GEMBASSY", "1.0.3");

  AjPSeqall seqall;
  AjPSeq    seq;
  AjPStr    inseq = NULL;

  AjBool at     = 0;
  AjBool purine = 0;
  AjBool keto   = 0;
  AjPStr method = 0;

  AjBool accid  = ajFalse;
  AjPStr restid = NULL;
  AjPStr seqid  = NULL;

  AjPStr base = NULL;
  AjPStr url  = NULL;

  AjPFile tmpfile = NULL;
  AjPStr  tmpname = NULL;
  AjPFilebuff tmp = NULL;

  AjPStr line = NULL;

  AjPFile outf = NULL;

  seqall = ajAcdGetSeqall("sequence");
  at     = ajAcdGetBoolean("at");
  purine = ajAcdGetBoolean("purine");
  keto   = ajAcdGetBoolean("keto");
  method = ajAcdGetSelectSingle("method");
  accid  = ajAcdGetBoolean("accid");
  outf   = ajAcdGetOutfile("outfile");

  base = ajStrNewC("rest.g-language.org");

  gAssignUniqueName(&tmpname);

  while(ajSeqallNext(seqall, &seq))
    {
      inseq = NULL;

      if(!accid)
        {
          if(gFormatGenbank(seq, &inseq))
            {
              tmpfile = ajFileNewOutNameS(tmpname);
              if(!tmpfile)
                {
                  ajDie("Output file (%S) open error\n", tmpname);
                }
              ajFmtPrintF(tmpfile, "%S", inseq);
              ajFileClose(&tmpfile);
              ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
              gFilePostSS(url, tmpname, &restid);
              ajStrDel(&url);
              ajSysFileUnlinkS(tmpname);
            }
          else
            {
              ajWarn("Sequence does not have features\n"
                     "Proceeding with sequence accession ID\n");
              accid = ajTrue;
            }
        }

      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      if(ajStrGetLen(seqid) == 0)
        {
          ajStrAssignS(&seqid, ajSeqGetNameS(seq));
        }

      if(ajStrGetLen(seqid) == 0)
        {
          ajWarn("No valid header information\n");
        }

      if(accid)
        {
          ajStrAssignS(&restid, seqid);
          if(ajStrGetLen(seqid) == 0)
            {
              ajDie("Cannot proceed without header with -accid\n");
            }

          if(!gValID(seqid))
            {
              ajDie("Invalid accession ID:%S, exiting\n", seqid);
            }
        }

      url = ajStrNew();

      ajFmtPrintS(&url, "http://%S/%S/delta_gcskew/", base, restid);

      if(!gFilebuffURLS(url, &tmp))
        {
          ajDie("Failed to download result from:\n%S\n", url);
        }

      ajBuffreadLine(tmp, &line);

      ajStrRemoveSetC(&line, "\n");

      ajFmtPrintF(outf, "Sequence: %S DELTA-GCskew %S\n", seqid, line);

      ajStrDel(&url);
      ajStrDel(&restid);
      ajStrDel(&seqid);
      ajStrDel(&inseq);
    }

  ajFileClose(&outf);

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&base);

  embExit();

  return 0;
}
Ejemplo n.º 3
0
int main(int argc, char *argv[])
{
  embInitPV("gcircularmap", argc, argv, "GEMBASSY", "1.0.1");

  struct soap soap;
  struct ns1__circular_USCOREmapInputParams params;

  AjPSeqall seqall;
  AjPSeq    seq;
  AjPStr    inseq    = NULL;
  AjPStr    seqid    = NULL;
  AjBool    accid    = ajFalse;
  AjPFile   outf     = NULL;
  AjPStr    filename = NULL;
  AjPStr    outfname = NULL;
  AjPStr    format   = NULL;

  ajint i;

  char *in0;
  char *result;

  seqall   = ajAcdGetSeqall("sequence");
  filename = ajAcdGetString("goutfile");
  accid    = ajAcdGetBoolean("accid");
  format   = ajAcdGetString("format");

  params.gmap = 0;

  i = 0;

  while(ajSeqallNext(seqall, &seq))
    {
      soap_init(&soap);

      soap.send_timeout = 0;
      soap.recv_timeout = 0;

      inseq = NULL;

      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      if(!ajStrGetLen(seqid))
        ajStrAssignS(&seqid, ajSeqGetNameS(seq));

      if(!ajStrGetLen(seqid))
        {
          ajWarn("No valid header information\n");
        }

      if(accid || !gFormatGenbank(seq, &inseq))
        {
          if(!accid)
            ajWarn("Sequence does not have features\n"
                   "Proceeding with sequence accession ID:%S\n", seqid);

          if(!gValID(seqid))
            {
              ajDie("Invalid accession ID:%S, exiting\n", seqid);
            }

          ajStrAssignS(&inseq, seqid);
        }

      in0 = ajCharNewS(inseq);

      if(soap_call_ns1__circular_USCOREmap(
	                                  &soap,
					   NULL,
					   NULL,
					   in0,
					  &params,
					  &result
                                          ) == SOAP_OK)
	{
          ++i;

          outfname = ajStrNewS(ajFmtStr("%S.%d.%S",
                                        filename,
                                        i,
                                        format));

          outf = ajFileNewOutNameS(outfname);

          if(!outf)
            {
              ajDie("File open error\n");
            }

          if(!ajStrMatchC(format, "svg"))
            {
              if(!gHttpConvertC(result, &outf, ajStrNewC("svg"), format))
                {
                  ajDie("File downloading error from:\n%s\n", result);
                }
              else
                {
                  ajFmtPrint("Created %S\n", outfname);
                }
            }
          else
            {
              if(!gHttpGetBinC(result, &outf))
                {
                  ajDie("File downloading error from:\n%s\n", result);
                }
              else
                {
                  ajFmtPrint("Created %S\n", outfname);
                }
            }

          ajStrDel(&outfname);
	}
      else
	{
	  soap_print_fault(&soap, stderr);
	}

      soap_destroy(&soap);
      soap_end(&soap);
      soap_done(&soap);

      AJFREE(in0);

      ajStrDel(&inseq);
    }

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&seqid);

  ajStrDel(&filename);

  embExit();

  return 0;
}
Ejemplo n.º 4
0
int main(int argc, char *argv[])
{
  embInitPV("gbasezvalue", argc, argv, "GEMBASSY", "1.0.3");

  AjPSeqall seqall;
  AjPSeq    seq;
  AjPStr    inseq = NULL;

  AjPStr position   = NULL;
  ajint	 limit      = 0;
  ajint  PatLen     = 0;
  ajint  upstream   = 0;
  ajint  downstream = 0;

  AjBool accid  = ajFalse;
  AjPStr restid = NULL;
  AjPStr seqid  = NULL;

  AjPStr base = NULL;
  AjPStr url  = NULL;

  AjPFile tmpfile = NULL;
  AjPStr  tmpname = NULL;

  AjPFile outf = NULL;

  seqall     = ajAcdGetSeqall("sequence");
  position   = ajAcdGetSelectSingle("position");
  limit      = ajAcdGetInt("limit");
  PatLen     = ajAcdGetInt("patlen");
  upstream   = ajAcdGetInt("upstream");
  downstream = ajAcdGetInt("downstream");
  accid      = ajAcdGetBoolean("accid");
  outf       = ajAcdGetOutfile("outfile");

  base = ajStrNewC("rest.g-language.org");

  gAssignUniqueName(&tmpname);

  while(ajSeqallNext(seqall, &seq))
    {
      inseq = NULL;

      if(!accid)
        {
          if(gFormatGenbank(seq, &inseq))
            {
              tmpfile = ajFileNewOutNameS(tmpname);
              if(!tmpfile)
                {
                  ajDie("Output file (%S) open error\n", tmpname);
                }
              ajFmtPrintF(tmpfile, "%S", inseq);
              ajFileClose(&tmpfile);
              ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
              gFilePostSS(url, tmpname, &restid);
              ajStrDel(&url);
              ajSysFileUnlinkS(tmpname);
            }
          else
            {
              ajWarn("Sequence does not have features\n"
                     "Proceeding with sequence accession ID\n");
              accid = ajTrue;
            }
        }

      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      if(ajStrGetLen(seqid) == 0)
        {
          ajStrAssignS(&seqid, ajSeqGetNameS(seq));
        }

      if(ajStrGetLen(seqid) == 0)
        {
          ajWarn("No valid header information\n");
        }

      if(accid)
        {
          ajStrAssignS(&restid, seqid);
          if(ajStrGetLen(seqid) == 0)
            {
              ajDie("Cannot proceed without header with -accid\n");
            }

          if(!gValID(seqid))
            {
              ajDie("Invalid accession ID:%S, exiting\n", seqid);
            }
        }

      url = ajStrNew();

      ajFmtPrintS(&url, "http://%S/%S/base_z_value/position=%S/PatLen=%d"
                  "upstream=%d/downstream=%d/limit=%d/output=f/tag=gene",
                  base, restid, position, PatLen, upstream, downstream, limit);

      ajFmtPrintF(outf, "Sequence: %S\n", seqid);
      if(!gFileOutURLS(url, &outf))
        {
          ajDie("Failed to download result from:\n%S\n", url);
        }

      ajStrDel(&url);
      ajStrDel(&restid);
      ajStrDel(&seqid);
      ajStrDel(&inseq);
    }

  ajFileClose(&outf);

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&base);

  ajStrDel(&position);

  embExit();

  return 0;
}
Ejemplo n.º 5
0
Archivo: gew.c Proyecto: ktnyt/GEMBASSY
int main(int argc, char *argv[])
{
  embInitPV("gew", argc, argv, "GEMBASSY", "1.0.3");

  AjPSeqall seqall;
  AjPSeq    seq;
  AjPStr    inseq = NULL;

  AjBool translate = ajFalse;
  AjPStr delkey    = NULL;

  AjBool accid  = ajFalse;
  AjPStr restid = NULL;
  AjPStr seqid  = NULL;

  AjPStr base = NULL;
  AjPStr url  = NULL;

  AjPFile tmpfile = NULL;
  AjPStr  tmpname = NULL;

  AjPFile outf = NULL;

  seqall    = ajAcdGetSeqall("sequence");
  translate = ajAcdGetBoolean("translate");
  delkey    = ajAcdGetString("delkey");
  accid     = ajAcdGetBoolean("accid");
  outf      = ajAcdGetOutfile("outfile");

  base = ajStrNewC("rest.g-language.org");

  gAssignUniqueName(&tmpname);

  while(ajSeqallNext(seqall, &seq))
    {
      inseq = NULL;

      if(!accid)
        {
          if(gFormatGenbank(seq, &inseq))
            {
              tmpfile = ajFileNewOutNameS(tmpname);
              if(!tmpfile)
                {
                  ajDie("Output file (%S) open error\n", tmpname);
                }
              ajFmtPrintF(tmpfile, "%S", inseq);
              ajFileClose(&tmpfile);
              ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
              gFilePostSS(url, tmpname, &restid);
              ajStrDel(&url);
              ajSysFileUnlinkS(tmpname);
            }
          else
            {
              ajWarn("Sequence does not have features\n"
                     "Proceeding with sequence accession ID\n");
              accid = ajTrue;
            }
        }

      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      if(ajStrGetLen(seqid) == 0)
        {
          ajStrAssignS(&seqid, ajSeqGetNameS(seq));
        }

      if(ajStrGetLen(seqid) == 0)
        {
          ajWarn("No valid header information\n");
        }

      if(accid)
        {
          ajStrAssignS(&restid, seqid);
          if(ajStrGetLen(seqid) == 0)
            {
              ajDie("Cannot proceed without header with -accid\n");
            }

          if(!gValID(seqid))
            {
              ajDie("Invalid accession ID:%S, exiting\n", seqid);
            }
        }

      url = ajStrNew();

      ajFmtPrintS(&url, "http://%S/%S/Ew/translate=%d/delkey=%S/"
                  "output=f/tag=gene", base, restid, translate, delkey);

      ajFmtPrintF(outf, "Sequence: %S\n", seqid);
      if(!gFileOutURLS(url, &outf))
        {
          ajDie("Failed to download result from:\n%S\n", url);
        }

      ajStrDel(&url);
      ajStrDel(&restid);
      ajStrDel(&seqid);
      ajStrDel(&inseq);
    }

  ajFileClose(&outf);

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&base);

  ajStrDel(&delkey);

  embExit();

  return 0;
}
Ejemplo n.º 6
0
int main(int argc, char *argv[])
{
  embInitPV("genret", argc, argv, "GEMBASSY", "1.0.3");

  AjPSeqall seqall;
  AjPSeq seq      = NULL;
  AjPStr inseq    = NULL;
  AjPStr gene     = NULL;
  AjPStr access   = NULL;
  AjBool accid    = ajTrue;
  AjPStr argument = NULL;
  AjPFile outfile = NULL;

  AjPStr seqid  = NULL;
  AjPStr restid = NULL;

  AjBool valid = ajFalse;
  AjBool isseq = ajFalse;
  AjBool isgbk = ajFalse;

  AjPFilebuff buff = NULL;
  AjPFile  tmpfile = NULL;
  AjPStr   tmpname = NULL;

  AjPStr regexstr = NULL;
  AjPStrTok token = NULL;
  AjPRegexp regex = NULL;

  AjPStr url  = NULL;
  AjPStr base = NULL;
  AjPStr head = NULL;
  AjPStr line = NULL;

  seqall   = ajAcdGetSeqall("sequence");
  access   = ajAcdGetString("access");
  gene     = ajAcdGetString("gene");
  argument = ajAcdGetString("argument");
  accid    = ajAcdGetBoolean("accid");
  outfile  = ajAcdGetOutfile("outfile");

  if(
     ajStrMatchC(access, "translation") ||
     ajStrMatchC(access, "get_exon") ||
     ajStrMatchC(access, "get_exons") ||
     ajStrMatchC(access, "get_cdsseq") ||
     ajStrMatchC(access, "get_gbkseq") ||
     ajStrMatchC(access, "get_geneseq") ||
     ajStrMatchC(access, "get_intron") ||
     ajStrMatchC(access, "getseq") ||
     ajStrMatchC(access, "seq") ||
     ajStrMatchC(access, "around_startcodon") ||
     ajStrMatchC(access, "around_stopcodon") ||
     ajStrMatchC(access, "before_startcodon") ||
     ajStrMatchC(access, "before_stopcodon") ||
     ajStrMatchC(access, "after_startcodon") ||
     ajStrMatchC(access, "after_stopcodon")
     )
    {
      isseq = ajTrue;
    }
  else if(ajStrMatchC(access, "annotate") ||
          ajStrMatchC(access, "output"))
    {
      isgbk = ajTrue;
    }
  else
    {
      ajFmtPrintF(outfile, "gene,%S\n", access);
    }

  base = ajStrNewC("rest.g-language.org");

  ajStrExchangeCC(&argument, " ", "/");
  ajStrExchangeCC(&argument, ",", "/");
  ajStrExchangeCC(&argument, "\t", "/");
  ajStrExchangeCC(&argument, "\r", "/");
  ajStrExchangeCC(&argument, "\n", "/");

  if(ajStrMatchC(gene, "*"))
    {
      ajStrInsertK(&gene, 0, '.');
    }

  if(ajStrPrefixC(gene, "@") || ajStrPrefixC(gene, "list::"))
    {
      ajStrExchangeCC(&gene, "@", "");
      ajStrExchangeCC(&gene, "list::", "");
      ajStrAssignS(&tmpname, gene);

      tmpfile = ajFileNewInNameS(tmpname);

      if(!tmpfile)
        {
          ajDie("List file (%S) open error\n", tmpname);
        }

      gene = ajStrNew();

      while(ajReadline(tmpfile, &line))
        {
          ajStrAppendS(&gene, line);
        }

      ajFileClose(&tmpfile);
      ajStrDel(&tmpname);
      ajStrDel(&line);
    }

  tmpname = ajStrNew();
  gAssignUniqueName(&tmpname);

  while(ajSeqallNext(seqall, &seq))
    {
      inseq = ajStrNew();

      if(!accid)
        {
          if(gFormatGenbank(seq, &inseq))
            {
              tmpfile = ajFileNewOutNameS(tmpname);

              if(!tmpfile)
                {
                  ajDie("Output file (%S) open error\n", tmpname);
                }

              ajFmtPrintF(tmpfile, "%S", inseq);

              ajFileClose(&tmpfile);

              ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);

              gFilePostSS(url, tmpname, &restid);

              ajStrDel(&url);

              ajSysFileUnlinkS(tmpname);
            }
          else
            {
              ajWarn("Sequence does not have features\n"
                     "Proceeding with sequence accession ID\n");
              accid = ajTrue;
            }
        }


      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      if(ajStrGetLen(seqid) == 0)
        {
          ajStrAssignS(&seqid, ajSeqGetNameS(seq));
        }

      if(ajStrGetLen(seqid) == 0)
        {
          ajWarn("No valid header information\n");
        }

      if(accid)
        {
          ajStrAssignS(&restid, seqid);
          if(ajStrGetLen(seqid) == 0)
            {
              ajDie("Cannot proceed without header with -accid\n");
            }

          if(!gValID(seqid))
            {
              ajDie("Invalid accession ID:%S, exiting\n", seqid);
            }
        }

      url = ajStrNew();

      if(isgbk)
        {
          ajFmtPrintS(&url, "http://%S/%S/%S", base, restid, access);
        }
      else
        {
          ajFmtPrintS(&url, "http://%S/%S/*/%S/%S", base, restid, access, argument);
        }

      if(!gFilebuffURLS(url, &buff))
        {
          ajDie("GET error from %S\n", url);
        }

      while(ajBuffreadLine(buff, &line))
        {
          if(isgbk){
            ajFmtPrintF(outfile, "%S", line);
            continue;
          }

          ajStrRemoveLastNewline(&line);

          regex = ajRegCompC("^>");

          if(ajRegExec(regex, line))
            {
              head = ajStrNew();

              ajStrAssignS(&head, line);
              ajStrTrimStartC(&head, ">");

              valid = ajFalse;

              token = ajStrTokenNewC(ajStrNewS(gene), " ,\t\r\n");

              while(ajStrTokenNextParse(token, &regexstr))
                {
                  if(ajStrGetLen(regexstr))
                    {
                      regex = ajRegComp(regexstr);

                      if(ajRegExec(regex, line))
                        {
                          valid = ajTrue;
                          if(ajStrIsAlnum(regexstr))
                            {
                              ajStrExchangeSC(&gene, regexstr, "");
                            }
                        }

                      ajRegFree(&regex);
                    }
                }
            }
          else
            {
              if(valid)
                {
                  if(isseq)
                    {
                      ajStrFmtWrap(&line, 60);
                      ajFmtPrintF(outfile, ">%S\n%S\n", head, line);
                    }
                  else
                    {
                      ajFmtPrintF(outfile, "%S,%S\n", head, line);
                    }

                  valid = ajFalse;
                }
            }
        }

      ajFileClose(&outfile);

      ajStrDel(&restid);
      ajStrDel(&seqid);
      ajStrDel(&inseq);
    }

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&access);
  ajStrDel(&gene);

  embExit();
}
Ejemplo n.º 7
0
int main(int argc, char *argv[])
{
  embInitPV("greporiter", argc, argv, "GEMBASSY", "1.0.3");

  AjPSeqall seqall;
  AjPSeq    seq;
  AjPStr    inseq  = NULL;

  AjBool accid  = ajFalse;
  AjPStr restid = NULL;
  AjPStr seqid  = NULL;

  AjPStr base = NULL;
  AjPStr url  = NULL;

  AjBool oriloc = 0;
  AjBool gcskew = 0;
  AjBool dbonly = 0;
  ajint	 difthreshold = 0;

  AjPFile outf = NULL;

  AjPFile     tmpfile = NULL;
  AjPStr      tmpname = NULL;
  AjPStr      fstname = NULL;
  AjPFilebuff tmp     = NULL;
  AjPStr      line    = NULL;
  AjPSeqout   tmpout  = NULL;

  AjPRegexp regex;

  AjPStr    ori    = NULL;
  AjPStr    ter    = NULL;

  seqall = ajAcdGetSeqall("sequence");
  difthreshold = ajAcdGetInt("difthreshold");
  oriloc = ajAcdGetBoolean("oriloc");
  gcskew = ajAcdGetBoolean("gcskew");
  dbonly = ajAcdGetBoolean("dbonly");
  accid  = ajAcdGetBoolean("accid");
  outf   = ajAcdGetOutfile("outfile");

  base = ajStrNewC("rest.g-language.org");

  gAssignUniqueName(&tmpname);
  gAssignUniqueName(&fstname);
  ajStrAppendC(&fstname, ".fasta");

  while(ajSeqallNext(seqall, &seq))
    {
      inseq = ajStrNew();

      tmpout = ajSeqoutNew();

      if(!accid)
        {
          if(gFormatGenbank(seq, &inseq))
            {
              tmpfile = ajFileNewOutNameS(tmpname);
              if(!tmpfile)
                {
                  ajDie("Output file (%S) open error\n", tmpname);
                }
              ajFmtPrintF(tmpfile, "%S", inseq);
              ajFileClose(&tmpfile);
              ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
              gFilePostSS(url, tmpname, &restid);
              ajStrDel(&url);
              ajSysFileUnlinkS(tmpname);
            }
          else
            {
              if(!ajSeqoutOpenFilename(tmpout, fstname))
                {
                  embExitBad();
                }

              ajSeqoutSetFormatS(tmpout,ajStrNewC("fasta"));
              ajSeqoutWriteSeq(tmpout, seq);
              ajSeqoutClose(tmpout);
              ajSeqoutDel(&tmpout);
              ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
              gFilePostSS(url, fstname, &restid);
              ajStrDel(&url);
              ajSysFileUnlinkS(fstname);
            }
        }

      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      if(ajStrGetLen(seqid) == 0)
        {
          ajStrAssignS(&seqid, ajSeqGetNameS(seq));
        }

      if(ajStrGetLen(seqid) == 0)
        {
          ajWarn("No valid header information\n");
        }

      if(accid)
        {
          ajStrAssignS(&restid, seqid);
          if(ajStrGetLen(seqid) == 0)
            {
              ajDie("Cannot proceed without header with -accid\n");
            }

          if(!gValID(seqid))
            {
              ajDie("Invalid accession ID:%S, exiting\n", seqid);
            }
        }

      url = ajStrNew();

      ajFmtPrintS(&url, "http://%S/%S/rep_ori_ter/oriloc=%d/gcskew=%d/"
                  "difthreshold=%d/dbonly=%d/",  base, restid, oriloc, gcskew,
                  difthreshold, dbonly);

      if(!gFilebuffURLS(url, &tmp))
        {
          ajDie("Failed to download result from:\n%S\n", url);
        }

      ajBuffreadLine(tmp, &line);

      regex = ajRegCompC("([0-9]+),([0-9]+)");

      if(ajRegExec(regex, line)) {
        if(ajRegSubI(regex, 1, &ori), ajRegSubI(regex, 2, &ter)) {
          ajFmtPrint("%S Origin: %S Terminus %S\n", seqid, ori, ter);
        }
      }

      ajStrDel(&url);
      ajStrDel(&restid);
      ajStrDel(&seqid);
      ajStrDel(&inseq);
    }

  ajFileClose(&outf);

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&base);

  embExit();

  return 0;
}