Ejemplo n.º 1
0
    bool
SimpleReadWriter::writeHeader(
    const ReaderContext& context,
    bool sorted,
    int argc,
    const char **argv,
    const char *version,
    const char *rgLine,
	bool omitSQLines)
{
    char* buffer;
    size_t size;
    size_t used;

    char *localBuffer = NULL;

	writer->inHeader(true);
    if (! writer->getBuffer(&buffer, &size)) {
        return false;
    }

    char *writerBuffer = buffer;
    size_t writerBufferSize = size;

	while (!format->writeHeader(context, buffer, size, &used, sorted, argc, argv, version, rgLine, omitSQLines)) {
        delete[] localBuffer;
        size = 2 * size;
        localBuffer = new char[size];
        buffer = localBuffer;
    }

    if (NULL == localBuffer) {
        _ASSERT(writerBuffer == buffer);
        writer->advance((unsigned)used, 0);
        writer->nextBatch();
    } else {
        size_t bytesRemainingToWrite = used;
        size_t bytesWritten = 0;
        while (bytesRemainingToWrite > 0) {
            size_t bytesToWrite = __min(bytesRemainingToWrite, writerBufferSize);
            memcpy(writerBuffer, localBuffer + bytesWritten, bytesToWrite);
            writer->advance(bytesToWrite);
            writer->nextBatch();
            if (!writer->getBuffer(&writerBuffer, &writerBufferSize)) {
                return false;
            }
            bytesWritten += bytesToWrite;
            bytesRemainingToWrite -= bytesToWrite;
        }

        delete[] localBuffer;
    }

	writer->inHeader(false);
    return true;
}
Ejemplo n.º 2
0
    void
GzipWriterFilterSupplier::onClosing(
    DataWriterSupplier* supplier)
{
    if (bamFormat) {
        closing = true;
        DataWriter* writer = supplier->getWriter();
        // write empty block as BAM end of file marker
        static _uint8 eof[] = {
            0x1f, 0x8b, 0x08, 0x04, 0x00, 0x00, 0x00, 0x00, 0x00, 0xff, 0x06, 0x00, 0x42, 0x43,
            0x02, 0x00, 0x1b, 0x00, 0x03, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00
        };
        char* buffer;
        size_t bytes;
        if (! (writer->getBuffer(&buffer, &bytes) && bytes >= sizeof(eof))) {
            WriteErrorMessage("no space to write eof marker\n");
            soft_exit(1);
        }
        memcpy(buffer, eof, sizeof(eof));
        writer->advance(sizeof(eof));

        // add final translation for last empty block
        writer->nextBatch();
        char* ignore;
        pair<_uint64,_uint64> last;
        size_t used;
        writer->getBatch(-1, &ignore, NULL, &used, (size_t*) &last.second, NULL, (size_t*) &last.first);
        last.second += used;
        translation.push_back(last);

        writer->close();
        delete writer;
    }

    // sort translations
    std::sort(translation.begin(), translation.end(), translationComparator);
}
Ejemplo n.º 3
0
    bool
SimpleReadWriter::writePairs(
    const ReaderContext& context, 
    Read **reads /* array of size NUM_READS_PER_PAIR */, 
    PairedAlignmentResult *result, 
    int nResults,
    SingleAlignmentResult **singleResults /* array of size NUM_READS_PER_PAIR*/, 
    int *nSingleResults /* array of size NUM_READS_PER_PAIR*/, 
    bool firstIsPrimary)
{
    bool retVal = false;
    //
    // We need to write all alignments for the pair into the same buffer, so that a write from
    // some other thread doesn't separate them.  We make two passes, trying to write into the 
    // existing buffer, and then into a clean one.  If that doesn't work, abort the alignment
    // run and ask for a bigger write buffer.
    //
    const int staticUsedBufferSize = 2000;
    size_t staticUsedBuffer[NUM_READS_PER_PAIR][staticUsedBufferSize];
    GenomeLocation staticLocationBuffer[NUM_READS_PER_PAIR][staticUsedBufferSize];

    GenomeLocation *finalLocations[NUM_READS_PER_PAIR];
    size_t *usedBuffer[NUM_READS_PER_PAIR];
    if (nResults + nSingleResults[0] <= staticUsedBufferSize && nResults + nSingleResults[1] <= staticUsedBufferSize) {
        usedBuffer[0] = staticUsedBuffer[0];
        usedBuffer[1] = staticUsedBuffer[1];
        finalLocations[0] = staticLocationBuffer[0];
        finalLocations[1] = staticLocationBuffer[1];
    } else {
        usedBuffer[0] = new size_t[nResults * NUM_READS_PER_PAIR + nSingleResults[0] + nSingleResults[1]];
        usedBuffer[1] = usedBuffer[0] + nResults + nSingleResults[0];
        finalLocations[0] = new GenomeLocation[nResults * NUM_READS_PER_PAIR + nSingleResults[0] + nSingleResults[1]];
        finalLocations[1] = finalLocations[0] + nResults + nSingleResults[0];
    }


    //
    // For paired reads, we need to have the same QNAME for both of them, and it needs to be unique among all other
    // reads in the dataset.  For now, all we do is see if the read names end in /1 and /2, and if so truncate them.
    //
    size_t idLengths[NUM_READS_PER_PAIR];
    idLengths[0] = reads[0]->getIdLength();
    idLengths[1] = reads[1]->getIdLength();
    if (idLengths[0] == idLengths[1] && idLengths[0] > 2 && reads[0]->getId()[idLengths[0]-2] == '/' && reads[1]->getId()[idLengths[0]-2] == '/') {
        char lastChar0, lastChar1;
        lastChar0 = reads[0]->getId()[idLengths[0] - 1];
        lastChar1 = reads[1]->getId()[idLengths[1] - 1];
        if ((lastChar0 == '1' || lastChar0 == '2') && (lastChar1 == '1' || lastChar1 == '2') && 
            lastChar0 != lastChar1) {
                idLengths[0] -= 2;
                idLengths[1] -= 2;
        }
    }

    for (int pass = 0; pass < 2; pass++) {

        char* buffer;
        size_t size;
        size_t used = 0;

        bool fitInBuffer = true;

        if (!writer->getBuffer(&buffer, &size)) {
            goto done;
        }

        //
        // Write all of the pair alignments into the buffer.
        //
        for (int whichAlignmentPair = 0; whichAlignmentPair < nResults; whichAlignmentPair++) {
            reads[0]->setAdditionalFrontClipping(0);
            reads[1]->setAdditionalFrontClipping(0);

            GenomeLocation locations[2];
            locations[0] = result[whichAlignmentPair].status[0] != NotFound ? result[whichAlignmentPair].location[0] : InvalidGenomeLocation;
            locations[1] = result[whichAlignmentPair].status[1] != NotFound ? result[whichAlignmentPair].location[1] : InvalidGenomeLocation;

            int writeOrder[2];  // The order in which we write the reads, which is just numerical by genome location.  SO writeOrder[0] gets written first, and writeOrder[1] second.

            if (locations[0] <= locations[1]) {
                writeOrder[0] = 0;
                writeOrder[1] = 1;
            } else {
                writeOrder[0] = 1;
                writeOrder[1] = 0;
            }

            bool secondReadLocationChanged;
            int cumulativePositiveAddFrontClipping[NUM_READS_PER_PAIR] = { 0, 0 };

            do {
                size_t tentativeUsed = 0;
                secondReadLocationChanged = false;

                for (int firstOrSecond = 0; firstOrSecond < NUM_READS_PER_PAIR; firstOrSecond++) {  // looping over the order in which the reads are written, not the order in which they arrived
                    int whichRead = writeOrder[firstOrSecond];
                    //
                    // Loop until we get a write with no additional front clipping.
                    //
                    int addFrontClipping = 0;

                    while (!format->writeRead(context, &lvc, buffer + used + tentativeUsed, size - used - tentativeUsed, &usedBuffer[firstOrSecond][whichAlignmentPair],
                        idLengths[whichRead], reads[whichRead], result[whichAlignmentPair].status[whichRead], result[whichAlignmentPair].mapq[whichRead], locations[whichRead], result[whichAlignmentPair].direction[whichRead],
                        whichAlignmentPair != 0 || !firstIsPrimary, &addFrontClipping, true, writeOrder[firstOrSecond] == 0,
                        reads[1 - whichRead], result[whichAlignmentPair].status[1 - whichRead], locations[1 - whichRead], result[whichAlignmentPair].direction[1 - whichRead],
                        result[whichAlignmentPair].alignedAsPair)) {

                        if (0 == addFrontClipping || locations[whichRead] == InvalidGenomeLocation) {
                            //
                            // We failed because we ran out of buffer.
                            //
                            goto blownBuffer;
                        }

                        if (1 == firstOrSecond) {
                            //
                            // If the location of the second read changed, we need to redo the first one as well, because it includes an offset to the second read
                            //
                            secondReadLocationChanged = true;
                        }

                        const Genome::Contig *originalContig = genome->getContigAtLocation(locations[whichRead]);
                        const Genome::Contig *newContig = genome->getContigAtLocation(locations[whichRead] + addFrontClipping);
                        if (newContig != originalContig || NULL == newContig || locations[whichRead] + addFrontClipping > originalContig->beginningLocation + originalContig->length - genome->getChromosomePadding()) {
                            //
                            // Altering this would push us over a contig boundary.  Just give up on the read.
                            //
                            result[whichAlignmentPair].status[whichRead] = NotFound;
                            result[whichAlignmentPair].location[whichRead] = InvalidGenomeLocation;
                            locations[whichRead] = InvalidGenomeLocation;
                        } else {
                            if (addFrontClipping > 0) {
                                cumulativePositiveAddFrontClipping[firstOrSecond] += addFrontClipping;
                                reads[whichRead]->setAdditionalFrontClipping(cumulativePositiveAddFrontClipping[firstOrSecond]);
                            }
                            locations[whichRead] += addFrontClipping;
                        }
                    } // While formatting didn't work
                    tentativeUsed += usedBuffer[firstOrSecond][whichAlignmentPair];
                } // for first or second read

            } while (secondReadLocationChanged);
            used += usedBuffer[0][whichAlignmentPair] + usedBuffer[1][whichAlignmentPair];

            //
            // Both reads are written into the buffer.  Save the final locations we used for when we commit.
            //
            for (int whichRead = 0; whichRead < NUM_READS_PER_PAIR; whichRead++) {
                finalLocations[whichRead][whichAlignmentPair] = locations[whichRead];
            }
        } // for each pair.

        //
        // Now write the single alignments.
        //
        for (int whichRead = 0; whichRead < NUM_READS_PER_PAIR; whichRead++) {
            for (int whichAlignment = 0; whichAlignment < nSingleResults[whichRead]; whichAlignment++) {
                int addFrontClipping;
                reads[whichRead]->setAdditionalFrontClipping(0);
                GenomeLocation location = singleResults[whichRead][whichAlignment].status != NotFound ? singleResults[whichRead][whichAlignment].location : InvalidGenomeLocation;
                int cumulativePositiveAddFrontClipping = 0;

                while (!format->writeRead(context, &lvc, buffer + used, size - used, &usedBuffer[whichRead][nResults + whichAlignment], reads[whichRead]->getIdLength(),
                    reads[whichRead], singleResults[whichRead][whichAlignment].status, singleResults[whichRead][whichAlignment].mapq, location, singleResults[whichRead][whichAlignment].direction,
                    true, &addFrontClipping)) {

                    if (0 == addFrontClipping) {
                        goto blownBuffer;
                    }

                    const Genome::Contig *originalContig = genome->getContigAtLocation(location);
                    const Genome::Contig *newContig = genome->getContigAtLocation(location + addFrontClipping);
                    if (newContig != originalContig || NULL == newContig || location + addFrontClipping > originalContig->beginningLocation + originalContig->length - genome->getChromosomePadding()) {
                        //
                        // Altering this would push us over a contig boundary.  Just give up on the read.
                        //
                        singleResults[whichRead][whichAlignment].status = NotFound;
                        location = InvalidGenomeLocation;
                    } else {
                        if (addFrontClipping > 0) {
                            cumulativePositiveAddFrontClipping += addFrontClipping;
                            reads[whichRead]->setAdditionalFrontClipping(cumulativePositiveAddFrontClipping);
                        }
                        location += addFrontClipping;
                    }
                } 

                finalLocations[whichRead][nResults + whichAlignment] = location;
                used += usedBuffer[whichRead][nResults + whichAlignment];
            } // For each single alignment of a read
        } // For each read

        //
        // They all fit into the buffer.
        //

        //
        // Commit the updates for the pairs.
        //
        for (int whichReadPair = 0; whichReadPair < nResults; whichReadPair++) {
            for (int firstOrSecond = 0; firstOrSecond < NUM_READS_PER_PAIR; firstOrSecond++) {
                // adjust for write order
                int writeFirstOrSecond = (!!firstOrSecond) ^ (finalLocations[0][whichReadPair] > finalLocations[1][whichReadPair]); // goofy looking !! converts int to bool
                writer->advance((unsigned)usedBuffer[firstOrSecond][whichReadPair],
                    finalLocations[writeFirstOrSecond][whichReadPair] == InvalidGenomeLocation ? finalLocations[1 - writeFirstOrSecond][whichReadPair] : finalLocations[writeFirstOrSecond][whichReadPair]);
            }
        }

        //
        // Now commit the updates for the single reads.
        //
        for (int whichRead = 0; whichRead < NUM_READS_PER_PAIR; whichRead++) {
            for (int whichAlignment = 0; whichAlignment < nSingleResults[whichRead]; whichAlignment++) {
                writer->advance((unsigned)usedBuffer[whichRead][nResults + whichAlignment], finalLocations[whichRead][nResults + whichAlignment]);
            }
        }

        retVal = true;
        break;

blownBuffer:
        if (pass > 0) {
            WriteErrorMessage("Unable to fit all alignments for one read pair into a single write buffer.  Increase the size of the write buffer with -wbs, or reduce the number of alignments with -om or -omax\n");
            WriteErrorMessage("Read id: '%.*s'\n", reads[0]->getIdLength(), reads[0]->getId());
            soft_exit(1);
        }

        if (!writer->nextBatch()) {
            goto done;
        }
            
    } // For each buffer full pass



done:
    if (usedBuffer[0] != staticUsedBuffer[0]) {
        delete[] usedBuffer[0];
        usedBuffer[0] = usedBuffer[1] = NULL;

        delete[] finalLocations[0];
        finalLocations[0] = finalLocations[1] = NULL;
    }

    reads[0]->setAdditionalFrontClipping(0);
    reads[1]->setAdditionalFrontClipping(0);

    return retVal;
}
Ejemplo n.º 4
0
    bool
SimpleReadWriter::writeReads(
    const ReaderContext& context, 
    Read *read, 
    SingleAlignmentResult *results, 
    int nResults,
    bool firstIsPrimary)
{
    char* buffer;
    size_t size;
    size_t used;
    bool result = false;

    for (int i = 0; i < nResults; i++) {
        if (results[i].status == NotFound) {
            results[i].location = InvalidGenomeLocation;
        }
    }

    //
    // We need to keep track of the offsets of all of the alignments in the output buffer so we can commit them.  However,
    // we want to avoid dynamic memory allocation as much as possible.  So, we have a static buffer on the stack that's big enough
    // for the great majority of cases, and then allocate dynamically if that's too small.  Makes for annoying, but efficient
    // code.
    //

    const int staticUsedBufferSize = 2000;
    size_t staticUsedBuffer[staticUsedBufferSize];

    GenomeLocation staticFinalLocationsBuffer[staticUsedBufferSize];

    size_t *usedBuffer;
    GenomeLocation *finalLocations;
    if (nResults <= staticUsedBufferSize) {
        usedBuffer = staticUsedBuffer;
        finalLocations = staticFinalLocationsBuffer;
    } else {
        usedBuffer = new size_t[nResults];
        finalLocations = new GenomeLocation[nResults];
    }


    for (int pass = 0; pass < 2; pass++) { // Make two passes, one with whatever buffer space is left and one with a clean buffer.
        bool blewBuffer = false;

        if (!writer->getBuffer(&buffer, &size)) {
            goto done;
        }

        used = 0;

        for (int whichResult = 0; whichResult < nResults; whichResult++) {
            int addFrontClipping = 0;
            read->setAdditionalFrontClipping(0);
            int cumulativeAddFrontClipping = 0;
            finalLocations[whichResult] = results[whichResult].location;

            while (!format->writeRead(context, &lvc, buffer + used, size - used, &usedBuffer[whichResult], read->getIdLength(), read, results[whichResult].status,
                results[whichResult].mapq, finalLocations[whichResult], results[whichResult].direction, (whichResult > 0) || !firstIsPrimary, &addFrontClipping)) {

                if (0 == addFrontClipping) {
                    blewBuffer = true;
                    break;
                }

                // redo if read modified (e.g. to add soft clipping, or move alignment for a leading I.
                const Genome::Contig *originalContig = results[whichResult].status == NotFound ? NULL
                    : genome->getContigAtLocation(results[whichResult].location);
                const Genome::Contig *newContig = results[whichResult].status == NotFound ? NULL
                    : genome->getContigAtLocation(results[whichResult].location + addFrontClipping);
                if (newContig == NULL || newContig != originalContig || finalLocations[whichResult] + addFrontClipping > originalContig->beginningLocation + originalContig->length - genome->getChromosomePadding()) {
                    //
                    // Altering this would push us over a contig boundary.  Just give up on the read.
                    //
                    results[whichResult].status = NotFound;
                    results[whichResult].location = InvalidGenomeLocation;
                    finalLocations[whichResult] = InvalidGenomeLocation;
                } else {
                    cumulativeAddFrontClipping += addFrontClipping;
                    if (addFrontClipping > 0) {
                        read->setAdditionalFrontClipping(cumulativeAddFrontClipping);
                    }
                    finalLocations[whichResult] = results[whichResult].location + cumulativeAddFrontClipping;
                }
            } // while formatting doesn't work

            if (blewBuffer) {
                break;
            }

            used += usedBuffer[whichResult];
            _ASSERT(used <= size);

            if (used > 0xffffffff) {
                 WriteErrorMessage("SimpleReadWriter:writeReads: used too big\n");
                 soft_exit(1);
            }
        } // for each result.

        if (!blewBuffer) {
            //
            // Everything worked OK.
            //
            for (int whichResult = 0; whichResult < nResults; whichResult++) {
                writer->advance((unsigned)usedBuffer[whichResult], finalLocations[whichResult]);
            }
            result = true;
            goto done;
        }

        if (pass == 1) {
            WriteErrorMessage("Failed to write into fresh buffer; trying providing the -wbs switch with a larger value\n");
            soft_exit(1);
        }

        if (!writer->nextBatch()) {
            goto done;
        }
    } // for each pass (i.e., not empty, empty buffer)
    
done:
    if (usedBuffer != staticUsedBuffer) {
        delete[] usedBuffer;
        usedBuffer = NULL;

        delete[] finalLocations;
        finalLocations = NULL;
    }

    read->setAdditionalFrontClipping(0);

    return result;
}