// Set top segments to be equal width and so that segment 1, 2, 3,
// etc. corresponds to parent segment 1, 2, 3, etc.
void setTopSegments(Genome *genome, hal_size_t width) {
TopSegmentIteratorPtr topIt = genome->getTopSegmentIterator();
hal_size_t n = genome->getNumTopSegments();
hal_index_t startPos = 0;
for (; topIt->getArrayIndex() < n; topIt->toRight(), startPos += width) {
topIt->setCoordinates(startPos, width);
topIt->tseg()->setParentIndex(topIt->getArrayIndex());
topIt->tseg()->setParentReversed(false);
topIt->tseg()->setBottomParseIndex(NULL_INDEX);
topIt->tseg()->setNextParalogyIndex(NULL_INDEX);
}
}
void Genome::copyTopSegments(Genome *dest) const
{
const Genome *inParent = getParent();
const Genome *outParent = dest->getParent();
TopSegmentIteratorConstPtr inTop = getTopSegmentIterator();
TopSegmentIteratorPtr outTop = dest->getTopSegmentIterator();
hal_size_t n = dest->getNumTopSegments();
assert(n == 0 || n == getNumTopSegments());
if (n == 0) {
// Nothing to do if there are no top segments.
return;
}
BottomSegmentIteratorConstPtr inParentBottomSegIt = inParent->getBottomSegmentIterator();
BottomSegmentIteratorConstPtr outParentBottomSegIt = outParent->getBottomSegmentIterator();
for (; (hal_size_t)inTop->getArrayIndex() < n; inTop->toRight(),
outTop->toRight())
{
hal_index_t genomePos = inTop->getStartPosition();
assert(genomePos != NULL_INDEX);
string inSeqName = getSequenceBySite(genomePos)->getName();
string outSeqName = dest->getSequenceBySite(genomePos)->getName();
// if (inSeqName != outSeqName) {
// stringstream ss;
// ss << "When copying top segments from " << getName() << " to " << dest->getName() << ": sequence " << inSeqName << " != " << outSeqName << " at site " << genomePos;
// throw hal_exception(ss.str());
// }
outTop->setCoordinates(inTop->getStartPosition(), inTop->getLength());
outTop->setParentIndex(inTop->getParentIndex());
outTop->setParentReversed(inTop->getParentReversed());
outTop->setBottomParseIndex(inTop->getBottomParseIndex());
outTop->setNextParalogyIndex(inTop->getNextParalogyIndex());
// Check that the sequences from the bottom segments we point to are the same. If not, correct the indices so that they are.
if (inTop->getParentIndex() != NULL_INDEX) {
inParentBottomSegIt->toParent(inTop);
const Sequence *inParentSequence = inParentBottomSegIt->getSequence();
const Sequence *outParentSequence = outParent->getSequence(inParentSequence->getName());
hal_index_t inParentSegmentOffset = inTop->getParentIndex() - inParentSequence->getBottomSegmentArrayIndex();
hal_index_t outParentSegmentIndex = inParentSegmentOffset + outParentSequence->getBottomSegmentArrayIndex();
outTop->setParentIndex(outParentSegmentIndex);
}
}
}
void GenomeCopyTest::createCallBack(Alignment *alignment) {
hal_size_t alignmentSize = alignment->getNumGenomes();
CuAssertTrue(_testCase, alignmentSize == 0);
// Hacky: Need a different alignment to test copying the bottom
// segments correctly. (the names of a node's children are used
// when copying bottom segments, and two genomes can't have the same
// name in the same alignment)
_path = getTempFile();
_secondAlignment =
AlignmentPtr(getTestAlignmentInstances(alignment->getStorageFormat(), _path, WRITE_ACCESS | CREATE_ACCESS));
Genome *ancGenome = alignment->addRootGenome("AncGenome", 0);
Genome *leafGenome = alignment->addLeafGenome("LeafGenome1", "AncGenome", 0);
// This genome will test copyDimensions, copyTopSegments,
// copyBottomSegments, copySequence, copyMetadata
Genome *copyRootGenome = _secondAlignment->addRootGenome("copyRootGenome", 0);
Genome *copyLeafGenome = _secondAlignment->addLeafGenome("LeafGenome1", "copyRootGenome", 0);
MetaData *ancMeta = ancGenome->getMetaData();
ancMeta->set("Young", "Jeezy");
vector<Sequence::Info> seqVec(1);
seqVec[0] = Sequence::Info("Sequence", 1000000, 0, 700000);
ancGenome->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 1000000, 5000, 0);
leafGenome->setDimensions(seqVec);
string ancSeq = "CAT";
hal_index_t n = ancGenome->getSequenceLength();
DnaIteratorPtr dnaIt = ancGenome->getDnaIterator();
for (; dnaIt->getArrayIndex() < n; dnaIt->toRight()) {
size_t i = dnaIt->getArrayIndex() % ancSeq.size();
dnaIt->setBase(ancSeq[i]);
}
dnaIt->flush();
n = leafGenome->getSequenceLength();
dnaIt = leafGenome->getDnaIterator();
for (; dnaIt->getArrayIndex() < n; dnaIt->toRight()) {
size_t i = dnaIt->getArrayIndex() % ancSeq.size();
dnaIt->setBase(ancSeq[i]);
}
dnaIt->flush();
TopSegmentIteratorPtr topIt = leafGenome->getTopSegmentIterator();
n = leafGenome->getNumTopSegments();
for (; topIt->getArrayIndex() < n; topIt->toRight()) {
topIt->setCoordinates(topIt->getArrayIndex(), 1);
topIt->tseg()->setParentIndex(3);
topIt->tseg()->setParentReversed(true);
topIt->tseg()->setBottomParseIndex(5);
if (topIt->getArrayIndex() != 6) {
topIt->tseg()->setNextParalogyIndex(6);
} else {
topIt->tseg()->setNextParalogyIndex(7);
}
}
BottomSegmentIteratorPtr botIt = ancGenome->getBottomSegmentIterator();
n = ancGenome->getNumBottomSegments();
for (; botIt->getArrayIndex() < n; botIt->toRight()) {
botIt->setCoordinates(botIt->getArrayIndex(), 1);
botIt->bseg()->setChildIndex(0, 3);
botIt->bseg()->setChildReversed(0, true);
botIt->bseg()->setTopParseIndex(5);
}
seqVec[0] = Sequence::Info("Sequence", 3300, 0, 1100);
copyRootGenome->setDimensions(seqVec);
seqVec[0] = Sequence::Info("Sequence", 3300, 2200, 0);
copyLeafGenome->setDimensions(seqVec);
string copySeq = "TAG";
dnaIt = copyRootGenome->getDnaIterator();
n = copyRootGenome->getSequenceLength();
for (; dnaIt->getArrayIndex() < n; dnaIt->toRight()) {
size_t i = dnaIt->getArrayIndex() % copySeq.size();
dnaIt->setBase(copySeq[i]);
}
dnaIt->flush();
dnaIt = copyLeafGenome->getDnaIterator();
n = copyLeafGenome->getSequenceLength();
for (; dnaIt->getArrayIndex() < n; dnaIt->toRight()) {
size_t i = dnaIt->getArrayIndex() % copySeq.size();
dnaIt->setBase(copySeq[i]);
}
dnaIt->flush();
topIt = copyLeafGenome->getTopSegmentIterator();
n = copyLeafGenome->getNumTopSegments();
for (; topIt->getArrayIndex() < n; topIt->toRight()) {
topIt->setCoordinates(7, 8);
topIt->tseg()->setParentIndex(9);
topIt->tseg()->setParentReversed(false);
topIt->tseg()->setBottomParseIndex(11);
if (topIt->getArrayIndex() != 12) {
topIt->tseg()->setNextParalogyIndex(12);
} else {
topIt->tseg()->setNextParalogyIndex(7);
}
}
botIt = copyRootGenome->getBottomSegmentIterator();
n = copyRootGenome->getNumBottomSegments();
for (; botIt->getArrayIndex() < n; botIt->toRight()) {
botIt->setCoordinates(6, 7);
botIt->bseg()->setChildIndex(0, 8);
botIt->bseg()->setChildReversed(0, false);
botIt->bseg()->setTopParseIndex(10);
}
ancGenome->copy(copyRootGenome);
leafGenome->copy(copyLeafGenome);
_secondAlignment->close();
}
