void initialiseFilters() {
// resamplers
transform = TransformType::New();
transform->SetIdentity();
volumeInterpolator = VolumeInterpolatorType::New();
maskVolumeInterpolator = MaskVolumeInterpolatorType::New();
resampler = ResamplerType::New();
resampler->SetInput( originalImage );
resampler->SetInterpolator( volumeInterpolator );
resampler->SetOutputSpacing( resamplerSpacing );
resampler->SetSize( resamplerSize );
resampler->SetTransform( transform );
resampler->SetDefaultPixelValue( 127 );
maskResampler = MaskResamplerType::New();
maskResampler->SetInput( originalMask );
maskResampler->SetInterpolator( maskVolumeInterpolator );
maskResampler->SetOutputSpacing( resamplerSpacing );
maskResampler->SetSize( resamplerSize );
maskResampler->SetTransform( transform );
// extract image filters
sliceExtractor = SliceExtractorType::New();
sliceExtractor->SetInput( resampler->GetOutput() );
maskSliceExtractor = MaskSliceExtractorType::New();
maskSliceExtractor->SetInput( maskResampler->GetOutput() );
// masks
for(unsigned int i=0; i<resamplerSize[2]; i++) {
masks2D.push_back( MaskType2D::New() );
}
}
void ResampleResult(const char* name) {
InterpolatorNN::Pointer resampleInterpolator = InterpolatorNN::New();
ResamplerType::Pointer resample = ResamplerType::New();
resample->SetTransform(_finalTransform);
resample->SetInput(_src);
resample->UseReferenceImageOn();
resample->SetReferenceImage(_dst);
resample->SetInterpolator(resampleInterpolator);
resample->Update();
ImageType::Pointer resampledImage = resample->GetOutput();
itkcmds::itkImageIO<ImageType> io;
io.WriteImageT(name, resampledImage);
}
// ------------------------------------------------------------------------
void ValveNormaliser::AlignValve(const ValveType::Pointer &input, ValveType::Pointer &output)
{
if(!output) output = ValveType::New();
ImageType::Pointer image = input->GetImage();
PointType p1 = input->GetP1();
PointType p2 = input->GetP2();
// tranlsation to the origin
m_Transform = TransformType::New();
m_Transform->SetCenter(p1);
TransformType::OutputVectorType axis;
for(unsigned int i = 0; i < 3; i++)
{
axis[i] = -image->GetDirection()(i,2);
}
itk::Vector<double, 3> vec1, vec2;
for(unsigned int i = 0; i < 3; i++)
{
vec1[i] = p2[i]-p1[i];
vec2[i] = image->GetDirection()(i,0);
}
vec1.Normalize();
vec2.Normalize();
double angle = acos(vec2*vec1);
itk::Vector<double,3> axis2 = itk::CrossProduct(vec1,vec2);
axis2.Normalize();
m_Transform->Rotate3D(axis, angle);
typedef itk::ResampleImageFilter<ImageType, ImageType> ResamplerType;
ResamplerType::Pointer resampler = ResamplerType::New();
resampler->SetInput(image);
resampler->SetTransform(m_Transform);
resampler->SetOutputParametersFromImage(image);
resampler->Update();
// create the output
if(!output) output = ValveLine<3>::New();
output->SetImage(resampler->GetOutput());
output->SetP1(m_Transform->TransformPoint(input->GetP1()));
output->SetP2(m_Transform->GetInverseTransform()->TransformPoint(input->GetP2()));
output->UpdateIndexs();
}
// ------------------------------------------------------------------------
void ValveNormaliser::UnNormalise()
{
ImageType::Pointer image = m_Valve->GetImage();
typedef itk::ResampleImageFilter<ImageType, ImageType> ResamplerType;
ResamplerType::Pointer resampler = ResamplerType::New();
resampler->SetInput(image);
resampler->SetTransform(m_Transform->GetInverseTransform());
resampler->SetOutputParametersFromImage(image);
resampler->Update();
m_Output = ValveType::New();
m_Output->SetImage(resampler->GetOutput());
m_Output->SetP1(m_Transform->TransformPoint(m_Valve->GetP1()));
m_Output->SetP2(m_Transform->TransformPoint(m_Valve->GetP2()));
m_Output->UpdateIndexs();
if(m_FlipPoints)
FlipPoints(m_Output, m_Output);
if(m_Flip)
FlipValve(m_Output, m_Output);
}
int main(int argc, char** argv )
{
/**
* /home/morgan/invaginatingleg/invagination/invagination_T0.ome.tif
* /home/morgan/invaginatingleg/invagination/invagination_T1.ome.tif
* /home/morgan/invaginatingleg/invagination/invagination_T2.ome.tif
* /home/morgan/invaginatingleg/invagination/invagination_T3.ome.tif
* /home/morgan/invaginationmasked/invaginated_T4.ome.tif
* /home/morgan/invaginationmasked/invaginated_T5.ome.tif
* /home/morgan/invaginationmasked/invaginated_T6.ome.tif
* /home/morgan/invaginationmasked/invaginated_T7.ome.tif
* /home/morgan/invaginationmasked/invaginated_T8.ome.tif
* /home/morgan/invaginationmasked/invaginated_T9.ome.tif
* /home/morgan/invaginationmasked/invaginated_T10.ome.tif
* /home/morgan/invaginationmasked/invaginated_T11.ome.tif
* /home/morgan/invaginationmasked/invaginated_T12.ome.tif
* /home/morgan/invaginationmasked/invaginated_T13.ome.tif
* /home/morgan/invaginationmasked/invaginated_T14.ome.tif
* /home/morgan/invaginationmasked/invaginated_T15.ome.tif
* /home/morgan/invaginationmasked/invaginated_T16.ome.tif
* /home/morgan/invaginationmasked/invaginated_T17.ome.tif
* /home/morgan/invaginationmasked/invaginated_T18.ome.tif
* /home/morgan/invaginationmasked/invaginated_T19.ome.tif
* /home/morgan/invaginationmasked/invaginated_T20.ome.tif
* /home/morgan/invaginationmasked/invaginated_T21.ome.tif
* /home/morgan/invaginationmasked/invaginated_T22.ome.tif
* output
*/
std::vector<std::string> inputFiles;
typedef float PixelType;
// Fixed Image Type
typedef itk::Image<PixelType,3> FixedImageType;
// Moving Image Type
typedef itk::Image<PixelType,3> MovingImageType;
typedef FixedImageType::SpacingType SpacingType;
SpacingType spacing;
//spacing[0]=0.3107403;
//spacing[1]=0.3107403;
//spacing[2]=0.739833;
spacing[0]=0.1569476;
spacing[1]=0.1569476;
spacing[2]=0.6209697;
// Transform Type
typedef itk::VersorRigid3DTransform< double > TransformType;
#if 0
inputFiles.push_back("/home/morgan/invaginatingleg/invagination/invagination_T0.ome.tif");
inputFiles.push_back("/home/morgan/invaginatingleg/invagination/invagination_T1.ome.tif");
typedef float VectorComponentType;
typedef itk::Vector< VectorComponentType, 3 > VectorType;
typedef itk::Image< VectorType, 3 > DeformationFieldType;
typedef itk::ImageFileReader<FixedImageType> FixedImageReaderType;
typedef itk::ImageFileReader<MovingImageType> MovingImageReaderType;
FixedImageReaderType::Pointer fixedImageReader=FixedImageReaderType::New();
MovingImageReaderType::Pointer movingImageReader=MovingImageReaderType::New();
fixedImageReader->SetFileName(inputFiles[0]);
movingImageReader->SetFileName(inputFiles[1]);
fixedImageReader->Update();
movingImageReader->Update();
FixedImageType::Pointer fixedImage = fixedImageReader->GetOutput();
MovingImageType::Pointer movingImage = movingImageReader->GetOutput();
fixedImage->SetSpacing(spacing);
movingImage->SetSpacing(spacing);
typedef itk::VersorRigid3DTransform< double > Rigid3DTransformType;
typedef itk::LandmarkBasedTransformInitializer< Rigid3DTransformType, FixedImageType, MovingImageType >
LandmarkBasedTransformInitializerType;
LandmarkBasedTransformInitializerType::Pointer landmarkBasedTransformInitializer =
LandmarkBasedTransformInitializerType::New();
// Create source and target landmarks.
typedef LandmarkBasedTransformInitializerType::LandmarkPointContainer LandmarkContainerType;
typedef LandmarkBasedTransformInitializerType::LandmarkPointType LandmarkPointType;
LandmarkContainerType fixedLandmarks;
LandmarkContainerType movingLandmarks;
LandmarkPointType fixedPoint;
LandmarkPointType movingPoint;
fixedPoint[0] = 37.510;
fixedPoint[1] = 34.685;
fixedPoint[2] = 52*spacing[2];
movingPoint[0] = 31.468;
movingPoint[1] = 27.593;
movingPoint[2] = 50*spacing[2];
fixedLandmarks.push_back( fixedPoint );
movingLandmarks.push_back( movingPoint );
fixedPoint[0] = 32.645;
fixedPoint[1] = 33.901;
fixedPoint[2] = 52*spacing[2];
movingPoint[0] = 25.888;
movingPoint[1] = 26.353;
movingPoint[2] = 50*spacing[2];
fixedLandmarks.push_back( fixedPoint );
movingLandmarks.push_back( movingPoint );
fixedPoint[0] = 35.784;
fixedPoint[1] = 37.354;
fixedPoint[2] = 52*spacing[2];
movingPoint[0] = 29.918;
movingPoint[1] = 29.918;
movingPoint[2] = 50*spacing[2];
fixedLandmarks.push_back( fixedPoint );
movingLandmarks.push_back( movingPoint );
fixedPoint[0] = 33.116;
fixedPoint[1] = 37.040;
fixedPoint[2] = 52*spacing[2];
movingPoint[0] = 26.818;
movingPoint[1] = 29.608;
movingPoint[2] = 50*spacing[2];
fixedLandmarks.push_back( fixedPoint );
movingLandmarks.push_back( movingPoint );
landmarkBasedTransformInitializer->SetFixedLandmarks( fixedLandmarks );
landmarkBasedTransformInitializer->SetMovingLandmarks( movingLandmarks );
Rigid3DTransformType::Pointer transform = Rigid3DTransformType::New();
transform->SetIdentity();
landmarkBasedTransformInitializer->SetTransform(transform);
landmarkBasedTransformInitializer->InitializeTransform();
typedef itk::ResampleImageFilter<FixedImageType, MovingImageType, double > ResampleFilterType;
ResampleFilterType::Pointer resampleFilter = ResampleFilterType::New();
resampleFilter->SetInput( movingImage );
resampleFilter->SetTransform( transform );
resampleFilter->SetSize( fixedImage->GetLargestPossibleRegion().GetSize() );
resampleFilter->SetOutputOrigin( fixedImage->GetOrigin() );
resampleFilter->SetOutputSpacing( fixedImage->GetSpacing() );
resampleFilter->SetOutputDirection( fixedImage->GetDirection() );
resampleFilter->SetDefaultPixelValue(0);
resampleFilter->GetOutput();
// Write the output
typedef itk::ImageFileWriter< MovingImageType > WriterType;
WriterType::Pointer writer = WriterType::New();
writer->SetInput ( resampleFilter->GetOutput() );
writer->SetFileName( "output.ome.tif" );
writer->Update();
#endif
for(int i=1;i<argc-1;i++){
inputFiles.push_back(argv[i]);
}
std::string outputPrefix=argv[argc-1];
int nImages = inputFiles.size();
std::vector<TransformType::Pointer >transforms(nImages);
for(int i=0;i<nImages;i++){
transforms[i]=TransformType::New();
}
transforms[0]->SetIdentity();
for(int i=1;i<nImages;i++){
/*
* Read files
*/
std::cout << "Registering " << inputFiles[i] <<" to " <<inputFiles[i-1] << std::endl;
typedef itk::ImageFileReader<FixedImageType> FixedImageReaderType;
typedef itk::ImageFileReader<MovingImageType> MovingImageReaderType;
FixedImageReaderType::Pointer fixedImageReader=FixedImageReaderType::New();
MovingImageReaderType::Pointer movingImageReader=MovingImageReaderType::New();
fixedImageReader->SetFileName(inputFiles[i-1]);
movingImageReader->SetFileName(inputFiles[i]);
fixedImageReader->Update();
movingImageReader->Update();
FixedImageType::Pointer fixedImage = fixedImageReader->GetOutput();
MovingImageType::Pointer movingImage = movingImageReader->GetOutput();
fixedImage->SetSpacing(spacing);
movingImage->SetSpacing(spacing);
FixedImageType::SizeType size= fixedImage->GetLargestPossibleRegion().GetSize();
FixedImageType::PointType origin;
origin[0]=size[0]*spacing[0]/2;
origin[1]=size[1]*spacing[1]/2;
origin[2]=size[2]*spacing[2]/2;
fixedImage->SetOrigin(origin);
movingImage->SetOrigin(origin);
RegisterPair<FixedImageType,MovingImageType,TransformType>(fixedImage,movingImage,transforms[i]);
}
typedef itk::ResampleImageFilter<MovingImageType,MovingImageType> ResamplerType;
ResamplerType::Pointer resampler = ResamplerType::New();
//transform->SetIdentity();
#if 0
TransformType::ParametersType initialParameters( transform->GetNumberOfParameters() );
initialParameters.Fill( 0 );
initialParameters[3] = 1.0;
initialParameters[4]=10;
initialParameters[5]=10;
initialParameters[6]=0;
transform->SetParameters(initialParameters);
#endif
for(int i=0;i<nImages;i++){
typedef itk::ImageFileReader<MovingImageType> MovingImageReaderType;
MovingImageReaderType::Pointer movingImageReader=MovingImageReaderType::New();
movingImageReader->SetFileName(inputFiles[i]);
movingImageReader->Update();
MovingImageType::Pointer movingImage = movingImageReader->GetOutput();
movingImage->SetSpacing(spacing);
MovingImageType::SizeType size = movingImage->GetLargestPossibleRegion().GetSize();
MovingImageType::SizeType resampledSize;
resampledSize[0]=1280;
resampledSize[1]=1280;
resampledSize[2]=150;
resampler->SetSize(resampledSize );
typedef itk::TranslationTransform<double,3> TranslationTransformType;
TranslationTransformType::Pointer translationTransform =
TranslationTransformType::New();
TranslationTransformType::OutputVectorType translation;
translation[0] = -20;//-(resampledSize[0]-size[0])/2*spacing[0];
translation[1] = -20;// -(resampledSize[1]-size[1])/2*spacing[1];
translation[2] = -20; //-(resampledSize[2]-size[2])/2*spacing[2];
translationTransform->Translate(translation);
typedef itk::CompositeTransform<double,3> CompositeType;
CompositeType::Pointer composite = CompositeType::New();
composite->AddTransform(translationTransform);
for(int k=1;k<=i;k++){
composite->AddTransform(transforms[k]);
}
std::cout << "Origin: " << movingImage->GetOrigin() << std::endl;
resampler->SetInput(movingImage);
resampler->SetTransform(composite);
resampler->SetOutputOrigin(movingImage->GetOrigin());
resampler->SetOutputSpacing( movingImage->GetSpacing() );
resampler->SetOutputDirection( movingImage->GetDirection() );
resampler->SetDefaultPixelValue( 0 );
resampler->Update();
std::stringstream ss;
ss << outputPrefix << "-T" << i << ".ome.tif";
std::string filename;
ss >> filename;
typedef itk::ImageFileWriter<MovingImageType> MovingImageWriterType;
MovingImageWriterType::Pointer movingImageWriter=MovingImageWriterType::New();
movingImageWriter->SetFileName(filename);
movingImageWriter->SetInput(resampler->GetOutput());
movingImageWriter->Update();
std::cout << transforms[i] << std::endl;
}
}