static bool read_touches_graph(const read_t *r, const dBGraph *db_graph,
LoadingStats *stats)
{
bool found = false;
BinaryKmer bkmer; Nucleotide nuc; dBNode node;
const size_t kmer_size = db_graph->kmer_size;
size_t i, num_contigs = 0, num_kmers_loaded = 0;
size_t search_pos = 0, start, end = 0, contig_len;
if(r->seq.end >= kmer_size)
{
while((start = seq_contig_start(r, search_pos, kmer_size, 0,0)) < r->seq.end &&
!found)
{
end = seq_contig_end(r, start, kmer_size, 0, 0, &search_pos);
contig_len = end - start;
__sync_fetch_and_add((volatile size_t*)&stats->total_bases_loaded, contig_len);
num_contigs++;
bkmer = binary_kmer_from_str(r->seq.b + start, kmer_size);
num_kmers_loaded++;
node = db_graph_find(db_graph, bkmer);
if(node.key != HASH_NOT_FOUND) { found = true; break; }
for(i = start+kmer_size; i < end; i++)
{
nuc = dna_char_to_nuc(r->seq.b[i]);
bkmer = binary_kmer_left_shift_add(bkmer, kmer_size, nuc);
num_kmers_loaded++;
node = db_graph_find(db_graph, bkmer);
if(node.key != HASH_NOT_FOUND) { found = true; break; }
}
}
}
// Update stats
__sync_fetch_and_add((volatile size_t*)&stats->total_bases_read, r->seq.end);
__sync_fetch_and_add((volatile size_t*)&stats->num_kmers_loaded, num_kmers_loaded);
__sync_fetch_and_add((volatile size_t*)&stats->num_kmers_novel, num_kmers_loaded - found);
__sync_fetch_and_add((volatile size_t*)&stats->num_good_reads, num_contigs > 0);
__sync_fetch_and_add((volatile size_t*)&stats->num_bad_reads, num_contigs == 0);
return found;
}
//
// Integrity checks
//
// Check an array of nodes denote a contigous path
bool db_node_check_nodes(const dBNode *nodes, size_t num,
const dBGraph *db_graph)
{
if(num == 0) return true;
const size_t kmer_size = db_graph->kmer_size;
BinaryKmer bkmer0, bkmer1, tmp;
Nucleotide nuc;
size_t i;
bkmer0 = db_node_oriented_bkmer(db_graph, nodes[0]);
for(i = 0; i+1 < num; i++)
{
bkmer1 = db_node_oriented_bkmer(db_graph, nodes[i+1]);
nuc = binary_kmer_last_nuc(bkmer1);
tmp = binary_kmer_left_shift_add(bkmer0, kmer_size, nuc);
ctx_assert_ret(binary_kmers_are_equal(tmp, bkmer1));
bkmer0 = bkmer1;
}
return true;
}
// Extend a supernode, nlist[offset] must already be set
// Walk along nodes starting from node/or, storing the supernode in nlist
// Returns the number of nodes added, adds no more than `limit`
// return false if out of space and limit > 0
bool supernode_extend(dBNodeBuffer *nbuf, size_t limit,
const dBGraph *db_graph)
{
ctx_assert(nbuf->len > 0);
const size_t kmer_size = db_graph->kmer_size;
dBNode node0 = nbuf->data[0], node1 = nbuf->data[nbuf->len-1], node = node1;
BinaryKmer bkmer = db_node_oriented_bkmer(db_graph, node);
Edges edges = db_node_get_edges_union(db_graph, node.key);
Nucleotide nuc;
while(edges_has_precisely_one_edge(edges, node.orient, &nuc))
{
bkmer = binary_kmer_left_shift_add(bkmer, kmer_size, nuc);
node = db_graph_find(db_graph, bkmer);
edges = db_node_get_edges_union(db_graph, node.key);
ctx_assert(node.key != HASH_NOT_FOUND);
if(edges_has_precisely_one_edge(edges, rev_orient(node.orient), &nuc))
{
if(node.key == node0.key || node.key == nbuf->data[nbuf->len-1].key) {
// don't create a loop A->B->A or a->b->B->A
break;
}
if(limit && nbuf->len >= limit) return false;
db_node_buf_add(nbuf, node);
}
else break;
}
return true;
}
// if colour is -1 aligns to all colours, otherwise aligns to given colour only
// Returns number of kmers lost from the end
static size_t db_alignment_from_read(dBAlignment *aln, const read_t *r,
uint8_t qcutoff, uint8_t hp_cutoff,
const dBGraph *db_graph, int colour)
{
size_t contig_start, contig_end = 0, search_start = 0;
const size_t kmer_size = db_graph->kmer_size;
BinaryKmer bkmer, tmp_key;
Nucleotide nuc;
hkey_t node;
size_t i, offset, nxtbse;
dBNodeBuffer *nodes = &aln->nodes;
Int32Buffer *rpos = &aln->rpos;
ctx_assert(nodes->len == rpos->len);
size_t n = nodes->len, init_len = n;
db_node_buf_capacity(nodes, n + r->seq.end);
int32_buf_capacity(rpos, n + r->seq.end);
while((contig_start = seq_contig_start(r, search_start, kmer_size,
qcutoff, hp_cutoff)) < r->seq.end)
{
contig_end = seq_contig_end(r, contig_start, kmer_size,
qcutoff, hp_cutoff, &search_start);
const char *contig = r->seq.b + contig_start;
size_t contig_len = contig_end - contig_start;
bkmer = binary_kmer_from_str(contig, kmer_size);
bkmer = binary_kmer_right_shift_one_base(bkmer);
for(offset=contig_start, nxtbse=kmer_size-1; nxtbse < contig_len; nxtbse++,offset++)
{
nuc = dna_char_to_nuc(contig[nxtbse]);
bkmer = binary_kmer_left_shift_add(bkmer, kmer_size, nuc);
tmp_key = binary_kmer_get_key(bkmer, kmer_size);
node = hash_table_find(&db_graph->ht, tmp_key);
if(node != HASH_NOT_FOUND &&
(colour == -1 || db_node_has_col(db_graph, node, colour)))
{
nodes->b[n].key = node;
nodes->b[n].orient = bkmer_get_orientation(bkmer, tmp_key);
rpos->b[n] = offset;
n++;
}
}
}
// Return number of bases from the last kmer found until read end
size_t ret = (n == init_len ? r->seq.end /* No kmers found */
: r->seq.end - (rpos->b[n-1] + kmer_size));
nodes->len = rpos->len = n;
// Check for sequence gaps
for(i = init_len; i+1 < nodes->len; i++) {
if(rpos->b[i]+1 < rpos->b[i+1]) {
aln->seq_gaps = true;
break;
}
}
return ret;
}