int gt_graphics_cairo_save_to_file(const GtGraphics *gg, const char *filename,
GtError *err)
{
const GtGraphicsCairo *g = (const GtGraphicsCairo*) gg;
cairo_surface_t *bgsurf = NULL;
cairo_t *bgc = NULL;
cairo_status_t rval;
GtFile *outfile;
gt_error_check(err);
gt_assert(g && filename);
/* do nothing if no surface was created */
if (g->from_context)
return 0;
switch (g->type)
{
case GT_GRAPHICS_PNG:
/* blend rendered image with background color */
bgsurf = cairo_image_surface_create(CAIRO_FORMAT_ARGB32, g->width,
g->height);
bgc = cairo_create(bgsurf);
cairo_set_source_rgba(bgc, g->bg_color.red, g->bg_color.green,
g->bg_color.blue, g->bg_color.alpha);
cairo_paint(bgc);
cairo_set_source_surface(bgc, g->surf, 0, 0);
cairo_paint(bgc);
rval = cairo_surface_write_to_png(bgsurf, filename);
gt_assert(rval == CAIRO_STATUS_SUCCESS ||
rval == CAIRO_STATUS_WRITE_ERROR);
if (rval == CAIRO_STATUS_WRITE_ERROR)
{
cairo_destroy(bgc);
cairo_surface_destroy(bgsurf);
gt_error_set(err, "an I/O error occurred while attempting "
"to write image file \"%s\"", filename);
return -1;
}
cairo_destroy(bgc);
cairo_surface_destroy(bgsurf);
break;
default:
cairo_show_page(g->cr);
cairo_surface_flush(g->surf);
cairo_surface_finish(g->surf);
outfile = gt_file_open(GT_FILE_MODE_UNCOMPRESSED, filename, "w+", err);
if (outfile)
{
gt_file_xwrite(outfile, gt_str_get_mem(g->outbuf),
gt_str_length(g->outbuf));
gt_file_delete(outfile);
} else return -1;
break;
}
return 0;
}
void vcfoutput_write(VcfOutput *v, ResultSet *r) {
gt_assert(v);
gt_assert(r);
GtStr *temp = gt_str_new();
unsigned long i =0;
unsigned long vcf_size = 0;
vcf_size = gt_str_array_size(resultset_get_vcf_array(r));
for(i=0;i<gt_str_array_size(resultset_get_vcf_array(r));i++) {
gt_str_set(temp, gt_str_array_get(resultset_get_vcf_array(r),i));
if(i == vcf_size-1) {
gt_str_append_cstr(temp,";");
if(resultset_get_exon(r) != 0) {
gt_str_append_cstr(temp,"EX;");
}
if(resultset_get_frms(r) != 0) {
gt_str_append_cstr(temp,"NSF;");
}
if(resultset_get_miss(r) != 0) {
gt_str_append_cstr(temp,"NSM;");
}
if(resultset_get_nons(r) != 0) {
gt_str_append_cstr(temp,"NSN;");
}
if(resultset_get_threeprime(r) != 0) {
gt_str_append_cstr(temp,"ASS;");
}
if(resultset_get_fiveprime(r) != 0) {
gt_str_append_cstr(temp,"DSS;");
}
}
gt_str_append_cstr(temp,"\t");
gt_file_xwrite(v->file,gt_str_get(temp),gt_str_length(temp));
gt_str_reset(temp);
}
gt_str_delete(temp);
}
static int write_pdom(GtLTRdigestFileOutStream *ls, GtArray *pdoms,
const char *pdomname, GT_UNUSED GtRegionMapping *rmap,
char *desc, GtError *err)
{
int had_err = 0;
GtFile *seqfile = NULL,
*alifile = NULL,
*aafile = NULL;
GtUword i = 0,
seq_length = 0;
GtStr *pdom_seq,
*pdom_aaseq;
gt_error_check(err);
pdom_seq = gt_str_new();
pdom_aaseq = gt_str_new();
/* get protein domain output file */
seqfile = (GtFile*) gt_hashmap_get(ls->pdomout_files, pdomname);
if (seqfile == NULL)
{
/* no file opened for this domain yet, do it */
char buffer[GT_MAXFILENAMELEN];
(void) snprintf(buffer, (size_t) (GT_MAXFILENAMELEN-1), "%s_pdom_%s.fas",
ls->fileprefix, pdomname);
seqfile = gt_file_xopen(buffer, "w+");
gt_hashmap_add(ls->pdomout_files, gt_cstr_dup(pdomname), seqfile);
}
/* get protein alignment output file */
if (ls->write_pdom_alignments)
{
alifile = (GtFile*) gt_hashmap_get(ls->pdomali_files, pdomname);
if (alifile == NULL)
{
/* no file opened for this domain yet, do it */
char buffer[GT_MAXFILENAMELEN];
(void) snprintf(buffer, (size_t) (GT_MAXFILENAMELEN-1), "%s_pdom_%s.ali",
ls->fileprefix, pdomname);
alifile = gt_file_xopen(buffer, "w+");
gt_hashmap_add(ls->pdomali_files, gt_cstr_dup(pdomname), alifile);
}
}
/* get amino acid sequence output file */
if (ls->write_pdom_aaseqs)
{
aafile = (GtFile*) gt_hashmap_get(ls->pdomaa_files, pdomname);
if (aafile == NULL)
{
/* no file opened for this domain yet, do it */
char buffer[GT_MAXFILENAMELEN];
(void) snprintf(buffer, (size_t) (GT_MAXFILENAMELEN-1),
"%s_pdom_%s_aa.fas",
ls->fileprefix, pdomname);
aafile = gt_file_xopen(buffer, "w+");
gt_hashmap_add(ls->pdomaa_files, gt_cstr_dup(pdomname), aafile);
}
}
if (gt_array_size(pdoms) > 1UL)
{
for (i=1UL; i<gt_array_size(pdoms); i++)
{
gt_assert(gt_genome_node_cmp(*(GtGenomeNode**)gt_array_get(pdoms, i),
*(GtGenomeNode**)gt_array_get(pdoms, i-1))
>= 0);
}
if (gt_feature_node_get_strand(*(GtFeatureNode**) gt_array_get(pdoms, 0UL))
== GT_STRAND_REVERSE)
{
gt_array_reverse(pdoms);
}
}
/* output protein domain data */
for (i=0;i<gt_array_size(pdoms);i++)
{
GtRange pdom_rng;
GtStr *ali,
*aaseq;
GtFeatureNode *fn;
int rval;
fn = *(GtFeatureNode**) gt_array_get(pdoms, i);
ali = gt_genome_node_get_user_data((GtGenomeNode*) fn, "pdom_alignment");
aaseq = gt_genome_node_get_user_data((GtGenomeNode*) fn, "pdom_aaseq");
pdom_rng = gt_genome_node_get_range((GtGenomeNode*) fn);
rval = gt_extract_feature_sequence(pdom_seq, (GtGenomeNode*) fn,
gt_symbol(gt_ft_protein_match), false,
NULL, NULL, rmap, err);
if (rval)
{
had_err = -1;
break;
}
if (ls->write_pdom_alignments && ali)
{
char buf[BUFSIZ];
/* write away alignment */
(void) snprintf(buf, BUFSIZ-1, "Protein domain alignment in translated "
"sequence for candidate\n'%s':\n\n",
desc);
gt_file_xwrite(alifile, buf, (size_t) strlen(buf) * sizeof (char));
gt_file_xwrite(alifile, gt_str_get(ali),
(size_t) gt_str_length(ali) * sizeof (char));
gt_file_xwrite(alifile, "---\n\n", 5 * sizeof (char));
}
if (ls->write_pdom_aaseqs && aaseq)
{
/* append amino acid sequence */
gt_str_append_str(pdom_aaseq, aaseq);
}
gt_genome_node_release_user_data((GtGenomeNode*) fn, "pdom_alignment");
gt_genome_node_release_user_data((GtGenomeNode*) fn, "pdom_aaseq");
seq_length += gt_range_length(&pdom_rng);
}
if (!had_err)
{
gt_fasta_show_entry(desc,
gt_str_get(pdom_seq),
seq_length,
GT_FSWIDTH,
seqfile);
if (ls->write_pdom_aaseqs)
{
gt_fasta_show_entry(desc,
gt_str_get(pdom_aaseq),
gt_str_length(pdom_aaseq),
GT_FSWIDTH,
aafile);
}
}
gt_str_delete(pdom_seq);
gt_str_delete(pdom_aaseq);
return had_err;
}
static int split_fasta_file(const char *filename, unsigned long max_filesize,
bool force, GtError *err)
{
GtFile *srcfp = NULL, *destfp = NULL;
GtStr *destfilename = NULL;
unsigned long filenum = 0, bytecount = 0, separator_pos;
int read_bytes, had_err = 0;
char buf[BUFSIZ];
gt_error_check(err);
gt_assert(filename && max_filesize);
/* open source file */
srcfp = gt_file_xopen(filename, "r");
gt_assert(srcfp);
/* read start characters */
if ((read_bytes = gt_file_xread(srcfp, buf, BUFSIZ)) == 0) {
gt_error_set(err, "file \"%s\" is empty", filename);
had_err = -1;
}
bytecount += read_bytes;
/* make sure the file is in fasta format */
if (!had_err && buf[0] != '>') {
gt_error_set(err, "file is not in FASTA format");
had_err = -1;
}
if (!had_err) {
/* open destination file */
destfilename = gt_str_new();
gt_str_append_cstr_nt(destfilename, filename,
gt_file_basename_length(filename));
gt_str_append_char(destfilename, '.');
gt_str_append_ulong(destfilename, ++filenum);
gt_str_append_cstr(destfilename,
gt_file_mode_suffix(gt_file_mode(srcfp)));
if (!(destfp = gt_outputfile_xopen_forcecheck(gt_str_get(destfilename), "w",
force, err))) {
had_err = -1;
}
if (!had_err)
gt_file_xwrite(destfp, buf, read_bytes);
while (!had_err &&
(read_bytes = gt_file_xread(srcfp, buf, BUFSIZ)) != 0) {
if (bytecount + read_bytes > max_filesize) {
int offset = bytecount < max_filesize ? max_filesize - bytecount : 0;
if ((separator_pos = buf_contains_separator(buf, offset, read_bytes))) {
separator_pos--;
gt_assert(separator_pos < read_bytes);
if (separator_pos)
gt_file_xwrite(destfp, buf, separator_pos);
/* close current file */
gt_file_delete(destfp);
/* open new file */
gt_str_reset(destfilename);
gt_str_append_cstr_nt(destfilename, filename,
gt_file_basename_length(filename));
gt_str_append_char(destfilename, '.');
gt_str_append_ulong(destfilename, ++filenum);
gt_str_append_cstr(destfilename,
gt_file_mode_suffix(gt_file_mode(srcfp)));
if (!(destfp =
gt_outputfile_xopen_forcecheck(gt_str_get(destfilename), "w",
force, err))) {
had_err = -1;
break;
}
bytecount = read_bytes - separator_pos; /* reset */
gt_assert(buf[separator_pos] == '>');
gt_file_xwrite(destfp, buf + separator_pos,
read_bytes - separator_pos);
continue;
}
}
bytecount += read_bytes;
gt_file_xwrite(destfp, buf, read_bytes);
}
}
/* free */
gt_str_delete(destfilename);
/* close current file */
gt_file_delete(destfp);
/* close source file */
gt_file_delete(srcfp);
return had_err;
}
static int gt_sketch_runner(int argc, const char **argv, int parsed_args,
void *tool_arguments, GT_UNUSED GtError *err)
{
GtSketchArguments *arguments = tool_arguments;
GtNodeStream *in_stream = NULL,
*add_introns_stream = NULL,
*gff3_out_stream = NULL,
*feature_stream = NULL,
*sort_stream = NULL,
*last_stream;
GtFeatureIndex *features = NULL;
const char *file;
char *seqid = NULL;
GtRange qry_range, sequence_region_range;
GtArray *results = NULL;
GtStyle *sty = NULL;
GtStr *prog, *defaultstylefile = NULL;
GtDiagram *d = NULL;
GtLayout *l = NULL;
GtImageInfo* ii = NULL;
GtCanvas *canvas = NULL;
GtUword height;
bool has_seqid;
int had_err = 0;
gt_error_check(err);
gt_assert(arguments);
prog = gt_str_new();
gt_str_append_cstr_nt(prog, argv[0],
gt_cstr_length_up_to_char(argv[0], ' '));
defaultstylefile = gt_get_gtdata_path(gt_str_get(prog), err);
gt_str_delete(prog);
if (!defaultstylefile)
had_err = -1;
if (!had_err) {
gt_str_append_cstr(defaultstylefile, "/sketch/default.style");
}
file = argv[parsed_args];
if (!had_err) {
/* create feature index */
features = gt_feature_index_memory_new();
parsed_args++;
/* create an input stream */
if (strcmp(gt_str_get(arguments->input), "gff") == 0)
{
in_stream = gt_gff3_in_stream_new_unsorted(argc - parsed_args,
argv + parsed_args);
if (arguments->verbose)
gt_gff3_in_stream_show_progress_bar((GtGFF3InStream*) in_stream);
} else if (strcmp(gt_str_get(arguments->input), "bed") == 0)
{
if (argc - parsed_args == 0)
in_stream = gt_bed_in_stream_new(NULL);
else
in_stream = gt_bed_in_stream_new(argv[parsed_args]);
} else if (strcmp(gt_str_get(arguments->input), "gtf") == 0)
{
if (argc - parsed_args == 0)
in_stream = gt_gtf_in_stream_new(NULL);
else
in_stream = gt_gtf_in_stream_new(argv[parsed_args]);
}
last_stream = in_stream;
/* create add introns stream if -addintrons was used */
if (arguments->addintrons) {
sort_stream = gt_sort_stream_new(last_stream);
add_introns_stream = gt_add_introns_stream_new(sort_stream);
last_stream = add_introns_stream;
}
/* create gff3 output stream if -pipe was used */
if (arguments->pipe) {
gff3_out_stream = gt_gff3_out_stream_new(last_stream, NULL);
last_stream = gff3_out_stream;
}
/* create feature stream */
feature_stream = gt_feature_stream_new(last_stream, features);
/* pull the features through the stream and free them afterwards */
had_err = gt_node_stream_pull(feature_stream, err);
gt_node_stream_delete(feature_stream);
gt_node_stream_delete(gff3_out_stream);
gt_node_stream_delete(sort_stream);
gt_node_stream_delete(add_introns_stream);
gt_node_stream_delete(in_stream);
}
if (!had_err) {
had_err = gt_feature_index_has_seqid(features,
&has_seqid,
gt_str_get(arguments->seqid),
err);
}
/* if seqid is empty, take first one added to index */
if (!had_err && strcmp(gt_str_get(arguments->seqid),"") == 0) {
seqid = gt_feature_index_get_first_seqid(features, err);
if (seqid == NULL) {
gt_error_set(err, "GFF input file must contain a sequence region!");
had_err = -1;
}
}
else if (!had_err && !has_seqid) {
gt_error_set(err, "sequence region '%s' does not exist in GFF input file",
gt_str_get(arguments->seqid));
had_err = -1;
}
else if (!had_err)
seqid = gt_str_get(arguments->seqid);
results = gt_array_new(sizeof (GtGenomeNode*));
if (!had_err) {
had_err = gt_feature_index_get_range_for_seqid(features,
&sequence_region_range,
seqid,
err);
}
if (!had_err) {
qry_range.start = (arguments->start == GT_UNDEF_UWORD ?
sequence_region_range.start :
arguments->start);
qry_range.end = (arguments->end == GT_UNDEF_UWORD ?
sequence_region_range.end :
arguments->end);
}
if (!had_err) {
if (arguments->verbose)
fprintf(stderr, "# of results: "GT_WU"\n", gt_array_size(results));
/* find and load style file */
if (!(sty = gt_style_new(err)))
had_err = -1;
if (gt_str_length(arguments->stylefile) == 0) {
gt_str_append_str(arguments->stylefile, defaultstylefile);
} else {
if (!had_err && gt_file_exists(gt_str_get(arguments->stylefile))) {
if (arguments->unsafe)
gt_style_unsafe_mode(sty);
}
else
{
had_err = -1;
gt_error_set(err, "style file '%s' does not exist!",
gt_str_get(arguments->stylefile));
}
}
if (!had_err)
had_err = gt_style_load_file(sty, gt_str_get(arguments->stylefile), err);
}
if (!had_err) {
/* create and write image file */
if (!(d = gt_diagram_new(features, seqid, &qry_range, sty, err)))
had_err = -1;
if (!had_err && arguments->flattenfiles)
gt_diagram_set_track_selector_func(d, flattened_file_track_selector,
NULL);
if (had_err || !(l = gt_layout_new(d, arguments->width, sty, err)))
had_err = -1;
if (!had_err)
had_err = gt_layout_get_height(l, &height, err);
if (!had_err) {
ii = gt_image_info_new();
if (strcmp(gt_str_get(arguments->format),"pdf")==0) {
canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_PDF,
arguments->width,
height, ii, err);
}
else if (strcmp(gt_str_get(arguments->format),"ps")==0) {
canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_PS,
arguments->width,
height, ii, err);
}
else if (strcmp(gt_str_get(arguments->format),"svg")==0) {
canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_SVG,
arguments->width,
height, ii, err);
}
else {
canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_PNG,
arguments->width,
height, ii, err);
}
if (!canvas)
had_err = -1;
if (!had_err) {
had_err = gt_layout_sketch(l, canvas, err);
}
if (!had_err) {
if (arguments->showrecmaps) {
GtUword i;
const GtRecMap *rm;
for (i = 0; i < gt_image_info_num_of_rec_maps(ii) ;i++) {
char buf[BUFSIZ];
rm = gt_image_info_get_rec_map(ii, i);
(void) gt_rec_map_format_html_imagemap_coords(rm, buf, BUFSIZ);
printf("%s, %s\n",
buf,
gt_feature_node_get_type(gt_rec_map_get_genome_feature(rm)));
}
}
if (arguments->use_streams) {
GtFile *outfile;
GtStr *str = gt_str_new();
gt_canvas_cairo_file_to_stream((GtCanvasCairoFile*) canvas, str);
outfile = gt_file_open(GT_FILE_MODE_UNCOMPRESSED, file, "w+", err);
if (outfile) {
gt_file_xwrite(outfile, gt_str_get_mem(str), gt_str_length(str));
gt_file_delete(outfile);
} else {
had_err = -1;
}
gt_str_delete(str);
} else {
had_err = gt_canvas_cairo_file_to_file((GtCanvasCairoFile*) canvas,
file,
err);
}
}
}
}
/* free */
gt_free(seqid);
gt_canvas_delete(canvas);
gt_layout_delete(l);
gt_image_info_delete(ii);
gt_style_delete(sty);
gt_diagram_delete(d);
gt_array_delete(results);
gt_str_delete(defaultstylefile);
gt_feature_index_delete(features);
return had_err;
}
