static
rc_t HelicosLoaderFmt_WriteData(HelicosLoaderFmt* self, uint32_t argc, const SRALoaderFile* const argv[], int64_t* spots_bad_count)
{
rc_t rc = 0;
uint32_t i;
HelicosFileInfo* files = NULL;
bool done;
if( (files = calloc(argc, sizeof(*files))) == NULL ) {
rc = RC(rcSRA, rcFormatter, rcReading, rcMemory, rcInsufficient);
}
for(i = 0; rc == 0 && i < argc; i++) {
HelicosFileInfo* file = &files[i];
HelicosFileInfo_init(file);
file->file = argv[i];
}
do {
done = true;
for(i = 0; rc == 0 && i < argc; i++) {
HelicosFileInfo* file = &files[i];
if( (rc = read_next_spot(self, file)) == 0 && file->ready ) {
done = false;
rc = SRAWriterHelicos_Write(self->writer, argv[0], &file->name, &file->sequence, &file->quality);
file->ready = false;
}
}
} while( rc == 0 && !done );
free(files);
return rc;
}
static
rc_t FastqLoaderFmt_WriteData(FastqLoaderFmt* self, uint32_t argc, const SRALoaderFile* const argv[], int64_t* spots_bad_count)
{
rc_t rc = 0;
uint32_t i, g = 0;
FastqFileInfo* files = NULL;
bool done;
static IlluminaSpot spot;
if( (files = calloc(argc, sizeof(*files))) == NULL ) {
rc = RC(rcSRA, rcFormatter, rcReading, rcMemory, rcInsufficient);
}
for(i = 0; rc == 0 && i < argc; i++) {
ExperimentQualityType qType;
FastqFileInfo* file = &files[i];
file->file = argv[i];
FileReadData_init(file->spot, false);
FileReadData_init(&file->spot[1], false);
if( (rc = SRALoaderFile_QualityScoringSystem(file->file, &qType)) == 0 &&
(rc = SRALoaderFile_QualityEncoding(file->file, &file->qualEnc)) == 0 &&
(rc = SRALoaderFile_AsciiOffset(file->file, &file->qualOffset)) == 0 ) {
file->qualType = ILLUMINAWRITER_COLMASK_NOTSET;
if( qType == eExperimentQualityType_Undefined ) {
qType = self->processing->quality_type;
file->qualOffset = self->processing->quality_offset;
}
switch(qType) {
case eExperimentQualityType_LogOdds:
case eExperimentQualityType_Other:
if( self->w454 != NULL || self->wIonTorrent != NULL ) {
rc = RC(rcSRA, rcFormatter, rcConstructing, rcParam, rcInvalid);
LOGERR(klogInt, rc, "quality type other than Phred is not supported for this PLATFORM");
}
file->qualMin = -40;
file->qualMax = 41;
file->qualType = ILLUMINAWRITER_COLMASK_QUALITY_LOGODDS1;
break;
default:
SRALoaderFile_LOG(file->file, klogWarn, rc,
"quality_scoring_system attribute not set for this file, using Phred as default", NULL);
case eExperimentQualityType_Phred:
file->qualType = ILLUMINAWRITER_COLMASK_QUALITY_PHRED;
file->qualMin = 0;
file->qualMax = (self->wIllumina) ? 61: 127;
break;
}
}
}
do {
done = true;
for(i = 0; rc == 0 && i < argc; i++) {
FastqFileInfo* file = &files[i];
if( (rc = read_next_spot(self, file)) != 0 || !file->spot->ready ) {
continue;
}
done = false;
#if _DEBUGGING
{{
FileReadData* ss = file->spot;
do {
DEBUG_MSG(3, ("file-%u: name:'%s', bc:%s, rd:%i, flt:%hu, seq '%.*s', qual %u bytes\n",
i + 1, ss->name.data, ss->barcode.data, ss->read.read_id, ss->read.filter,
ss->read.seq.len, ss->read.seq.data, ss->read.qual.len));
if( ss == &file->spot[1]){ break; }
ss = file->spot[1].ready ? &file->spot[1] : NULL;
} while( ss != NULL );
}}
#endif
}
if( rc != 0 || done ) {
break;
}
/* collect spot reads, matching by spot name
* spot data may be split across multiple files
*/
IlluminaSpot_Init(&spot);
for(i = 0; rc == 0 && i < argc; i++) {
FileReadData* fspot = files[i].spot[0].ready ? &files[i].spot[0] : NULL;
while(rc == 0 && fspot != NULL ) {
rc = IlluminaSpot_Add(&spot, &fspot->name, &fspot->barcode, &fspot->read);
if( rc == 0 ) {
g = i;
fspot->ready = false;
} else if( GetRCState(rc) == rcIgnored ) {
rc = 0;
} else {
SRALoaderFile_LOG(files[i].file, klogErr, rc, "$(msg)", "msg=adding data to spot");
}
if( fspot == &files[i].spot[1]) { break; }
fspot = files[i].spot[1].ready ? &files[i].spot[1] : NULL;
}
}
if( rc == 0 ) {
if( self->wIllumina != NULL ) {
if( (rc = SRAWriterIllumina_Write(self->wIllumina, argv[0], &spot)) != 0 &&
GetRCTarget(rc) == rcFormatter && GetRCContext(rc) == rcValidating ) {
SRALoaderFile_LOG(files[g].file, klogWarn, rc, "$(msg) $(spot_name)", "msg=bad spot,spot_name=%.*s",
spot.name->len, spot.name->data);
self->spots_bad_count++;
if( self->spots_bad_allowed < 0 ||
self->spots_bad_count <= self->spots_bad_allowed ) {
rc = 0;
}
}
} else if( spot.nreads != 1 ) {
rc = RC(rcSRA, rcFormatter, rcReading, rcData, rcUnsupported);
SRALoaderFile_LOG(files[g].file, klogErr, rc, "$(msg)", "msg=multiple reads for this platform");
} else if( self->wIonTorrent != NULL ) {
rc = SRAWriterIonTorrent_WriteRead(self->wIonTorrent, argv[0], spot.name,
spot.reads[0].seq, spot.reads[0].qual, NULL, NULL, 0, 0, 0, 0);
} else {
rc = SRAWriter454_WriteRead(self->w454, argv[0], spot.name,
spot.reads[0].seq, spot.reads[0].qual, NULL, NULL, 0, 0, 0, 0);
}
}
} while( rc == 0 );
free(files);
*spots_bad_count = self->spots_bad_count;
return rc;
}
static
rc_t IlluminaLoaderFmt_WriteData(IlluminaLoaderFmt* self, uint32_t argc, const SRALoaderFile* const argv[], int64_t* spots_bad_count)
{
rc_t rc = 0;
uint32_t t, i, k, ftype_q = sizeof(file_types) / sizeof(file_types[0]);
SLList files;
IlluminaFileInfo* file = NULL;
SLListInit(&files);
/* group files using spotname, for _prb. file name prefix is used,
files reviewed by type detected from name and ordered by file_type array */
for(t = 0; rc == 0 && t < ftype_q; t++) {
for(i = 0; rc == 0 && i < argc; i++) {
const char* fname, *blk_pfx;
int prefix_len = 0;
ERunFileType ftype;
EIlluminaNativeFileType type = eIlluminaNativeFileTypeNotSet;
FGroup_Find_data data;
if( (rc = SRALoaderFileName(argv[i], &fname)) != 0 ) {
SRALoaderFile_LOG(argv[i], klogErr, rc, "reading file name", NULL);
break;
}
if( (rc = SRALoaderFile_FileType(argv[i], &ftype)) != 0 ) {
SRALoaderFile_LOG(argv[i], klogErr, rc, "reading file type", NULL);
break;
}
if( (rc = SRALoaderFileBlockName(argv[i], &blk_pfx)) != 0 ) {
SRALoaderFile_LOG(argv[i], klogErr, rc, "reading DATA_BLOCK/@name", NULL);
break;
}
if( blk_pfx == NULL ) {
blk_pfx = "";
}
{{
/* skip path if present */
const char* p = strrchr(fname, '/');
fname = p ? p + 1 : fname;
p = NULL;
for(k = 0; type == eIlluminaNativeFileTypeNotSet && k < ftype_q; k++) {
const char* const* e = file_types[k].key;
while( *e != NULL ) {
p = strstr(fname, *e++);
if( p != NULL ) {
type = file_types[k].type;
break;
}
}
}
if( p != NULL ) {
prefix_len = p - fname;
}
}}
if( ftype == rft_IlluminaNativeSeq ) {
type = eIlluminaNativeFileTypeFasta;
} else if( ftype == rft_IlluminaNativePrb ) {
type = eIlluminaNativeFileTypeQuality4;
} else if( ftype == rft_IlluminaNativeInt ) {
type = eIlluminaNativeFileTypeIntensity;
} else if( ftype == rft_IlluminaNativeQseq ) {
type = eIlluminaNativeFileTypeQSeq;
}
if( type == eIlluminaNativeFileTypeNotSet ) {
rc = RC(rcSRA, rcFormatter, rcReading, rcData, rcUnrecognized);
SRALoaderFile_LOG(argv[i], klogErr, rc, "detecting file type by file name", NULL);
break;
}
if( type != file_types[t].type ) {
/* one type at a time */
continue;
}
DEBUG_MSG(3, ("file '%s' type set to %d\n", fname, type));
file = calloc(1, sizeof(*file));
if( file == NULL ) {
rc = RC(rcSRA, rcFormatter, rcReading, rcMemory, rcExhausted);
SRALoaderFile_LOG(argv[i], klogErr, rc, "allocating file object", NULL);
break;
}
IlluminaFileInfo_init(file);
file->file = argv[i];
file->type = type;
if( file->type == eIlluminaNativeFileTypeQuality4 ) {
/* in _prb there is no spotname inside so use file prefix */
rc = pstring_assign(&data.key, fname, prefix_len);
} else {
/* try to get 1st spot so group can be organized by spot name */
if( (rc = read_next_spot(blk_pfx, file)) != 0 || !file->ready ) {
rc = rc ? rc : RC(rcSRA, rcFormatter, rcReading, rcData, rcNotFound);
SRALoaderFile_LOG(argv[i], klogErr, rc, "reading 1st spot", NULL);
break;
}
rc = pstring_copy(&data.key, &file->name);
}
data.found = NULL;
if( SLListDoUntil(&files, FGroup_Find, &data) && data.found != NULL ) {
IlluminaFileInfo* ss = data.found->files;
while( rc == 0 && file != NULL ) {
if( ss->type != eIlluminaNativeFileTypeQSeq && ss->type == file->type ) {
rc = RC(rcSRA, rcFormatter, rcReading, rcFile, rcDuplicate);
SRALoaderFile_LOG(argv[i], klogErr, rc, "type of file for lane", NULL);
} else if( ss->next != NULL ) {
ss = ss->next;
} else {
ss->next = file;
file->prev = ss;
data.found->mask |= file->type;
file = NULL;
}
}
} else {
data.found = calloc(1, sizeof(*data.found));
if( data.found == NULL ) {
rc = RC(rcSRA, rcFormatter, rcReading, rcMemory, rcInsufficient);
SRALoaderFile_LOG(argv[i], klogErr, rc, "preparing file group", NULL);
break;
} else {
if( (rc = pstring_assign(&data.found->key, fname, prefix_len)) != 0 ) {
SRALoaderFile_LOG(argv[i], klogErr, rc, "setting file group key", NULL);
FGroup_Whack(&data.found->dad, NULL);
break;
} else {
FGroup* curr = (FGroup*)SLListHead(&files), *prev = NULL;
data.found->blk_pfx = blk_pfx;
data.found->files = file;
data.found->mask = file->type;
/* group inserted into list by coords in 1st spot */
while( curr != NULL ) {
if( curr->files[0].coord[0] > file->coord[0] ||
(curr->files[0].coord[0] == file->coord[0] &&
curr->files[0].coord[1] > file->coord[1]) ) {
data.found->dad.next = &curr->dad;
if( prev == NULL ) {
files.head = &data.found->dad;
} else {
prev->dad.next = &data.found->dad;
}
break;
}
prev = curr;
curr = (FGroup*)curr->dad.next;
}
if( curr == NULL ) {
SLListPushTail(&files, &data.found->dad);
}
file = NULL;
}
}
}
}
}
if( rc == 0 ) {
SLListForEach(&files, FGroup_Validate, &rc);
}
if( rc == 0 ) {
FGroup_Parse_data data;
data.self = self;
if( SLListDoUntil(&files, FGroup_Parse, &data) ) {
rc = data.rc;
}
} else {
free(file);
}
SLListWhack(&files, FGroup_Whack, NULL);
*spots_bad_count = self->spots_bad_count;
return rc;
}
bool FGroup_Parse( SLNode *n, void *d )
{
FGroup_Parse_data* data = (FGroup_Parse_data*)d;
FGroup* g = (FGroup*)n;
bool done;
const SRALoaderFile* data_block_ref = NULL;
data->rc = 0;
do {
IlluminaFileInfo* file = g->files;
done = true;
while( data->rc == 0 && file != NULL ) {
if( (data->rc = read_next_spot(g->blk_pfx, file)) == 0 && file->ready ) {
done = false;
}
file = file->next;
}
if( data->rc != 0 || done ) {
break;
}
/* collect spot reads, matching by spot name
* spot data may be split across multiple files
*/
IlluminaSpot_Init(&data->spot);
file = g->files;
while( data->rc == 0 && file != NULL ) {
if( file->ready ) {
if( (file->type == eIlluminaNativeFileTypeNoise && data->self->skip_noise) ||
(file->type == eIlluminaNativeFileTypeIntensity && data->self->skip_intensity) ||
(file->type == eIlluminaNativeFileTypeSignal && data->self->skip_signal) ) {
file->ready = false;
} else {
data_block_ref = file->file;
if( file->type == eIlluminaNativeFileTypeQSeq && (g->mask & eIlluminaNativeFileTypeQuality4) ) {
/* drop quality1 from qseq data */
pstring_clear(&file->read.qual);
} else if( file->type == eIlluminaNativeFileTypeQuality4 ) {
IlluminaFileInfo* neib = file->next ? file->next : file->prev;
/* need to fix spotname to be same cause prb do not have any name in it */
if( (data->rc = pstring_copy(&file->name, &neib->name)) != 0 ) {
SRALoaderFile_LOG(file->file, klogErr, data->rc, "$(msg) '$(n)'", "msg=syncing prb spot name,n=%s", neib->name.data);
}
}
if( data->rc == 0 ) {
data->rc = IlluminaSpot_Add(&data->spot, &file->name, &file->barcode, &file->read);
if( data->rc == 0 ) {
file->ready = false;
} else {
if( GetRCState(data->rc) == rcIgnored ) {
SRALoaderFile_LOG(file->file, klogWarn, data->rc, "$(msg) '$(s1)' <> '$(s2)'",
"msg=spot name mismatch,s1=%.*s,s2=%.*s",
data->spot.name->len, data->spot.name->data, file->name.len, file->name.data);
data->self->spots_bad_count++;
/* skip spot for all files in a group */
file = g->files;
while( file != NULL ) {
file->ready = false;
SRALoaderFile_LOG(file->file, klogWarn, data->rc,
"$(msg) '$(n)'", "msg=skipped spot,n=%s", file->name.data);
file = file->next;
}
if( data->self->spots_bad_allowed >= 0 &&
data->self->spots_bad_count > data->self->spots_bad_allowed ) {
data->rc = RC(rcSRA, rcFormatter, rcReading, rcData, rcInvalid);
}
break;
}
}
}
}
}
file = file->next;
}
if( GetRCState(data->rc) == rcIgnored ) {
data->rc = 0;
continue;
}
if( data->rc == 0 ) {
data->rc = SRAWriterIllumina_Write(data->self->writer, data_block_ref, &data->spot);
}
} while( data->rc == 0 );
return data->rc != 0;
}
