Exemple #1
0
PhyloSummary::PhyloSummary(string refTfile, GroupMap* g, bool r, int p){
	try {
		m = MothurOut::getInstance();
		maxLevel = 0;
		ignore = false;
        numSeqs = 0;
        relabund = r;
        printlevel = p;
		
		groupmap = g;
        ct = NULL;
				
		//check for necessary files
		string taxFileNameTest = m->getFullPathName((refTfile.substr(0,refTfile.find_last_of(".")+1) + "tree.sum"));
		ifstream FileTest(taxFileNameTest.c_str());
		
		if (!FileTest) { 
			m->mothurOut("Error: can't find " + taxFileNameTest + "."); m->mothurOutEndLine(); exit(1);
		}else{
			readTreeStruct(FileTest);
		}
		
		tree[0].rank = "0";
		assignRank(0);

	}
	catch(exception& e) {
		m->errorOut(e, "PhyloSummary", "PhyloSummary");
		exit(1);
	}
}
Exemple #2
0
PhyloSummary::PhyloSummary(string refTfile, CountTable* c){
	try {
		m = MothurOut::getInstance();
		maxLevel = 0;
		ignore = false;
        numSeqs = 0;
		
		ct = c;
        groupmap = NULL;
        
		//check for necessary files
        if (refTfile == "saved") { ReferenceDB* rdb = ReferenceDB::getInstance(); refTfile = rdb->getSavedTaxonomy(); }
		string taxFileNameTest = m->getFullPathName((refTfile.substr(0,refTfile.find_last_of(".")+1) + "tree.sum"));
		ifstream FileTest(taxFileNameTest.c_str());
		
		if (!FileTest) { 
			m->mothurOut("Error: can't find " + taxFileNameTest + "."); m->mothurOutEndLine(); exit(1);
		}else{
			readTreeStruct(FileTest);
		}
		
		tree[0].rank = "0";
		assignRank(0);
        
	}
	catch(exception& e) {
		m->errorOut(e, "PhyloSummary", "PhyloSummary");
		exit(1);
	}
}
/** Diese Funktion prueft ob die Datei mit gewuenschten Namen bereits existiert. 
  * Wenn der Name bereits vergeben wurde, so hat der Nutzer die Moeglichkeit einen neuen Namen zu vergeben 
  * oder die bereits bestehende Datei zu ueberschreiben.
  * @param name Der Dateiname.
  */
string Magische_Quadrate::ueberpruefe_ob_file_existiert(string name)
{
  string file_name = name;
  bool ok = false;
  do
  {
    ifstream FileTest(file_name.c_str());
    if (FileTest && ok == false)
    {
      int eingabe = 0;
      cout << "\nDie Datei " + file_name + " existiert bereits!\n";
      cout << "Soll die Datei ueberschrieben werden [0] oder moechten Sie eine neue angeben[1]?\n";
      cout << "Ihre Wahl: ";
      eingabe = erfasse_int(0,1);
      if (eingabe == 1)
      {
        cout << "\nBitte geben Sie den Namen Ihrer neuen Datei an: ";
        erfasse_zeichenkette(file_name);
      }
      else
        ok = true;
    }
    else
    {
      ok = true;
      FileTest.close();
    }
  } while (!ok);
  return file_name;
}
bool pruefe_ob_datei_vorhanden(char *dateiname) {
	bool datei_vorhanden = true;
	std::ifstream FileTest(dateiname);
	if(!FileTest) {
		std::cout << "FEHLER: Datei existiert nicht." << std::endl;
		datei_vorhanden = false;
	}
	return datei_vorhanden;
}
Exemple #5
0
static void Handle_FileTest
(
    le_msg_MessageRef_t _msgRef
)
{
    // Get the message buffer pointer
    __attribute__((unused)) uint8_t* _msgBufPtr = ((_Message_t*)le_msg_GetPayloadPtr(_msgRef))->buffer;

    // Unpack the input parameters from the message
    int dataFile;
    dataFile = le_msg_GetFd(_msgRef);

    // Call the function
    FileTest ( (ServerCmdRef_t)_msgRef, dataFile );
}
Exemple #6
0
//*****************************************************************************
//                            MAIN FUNCTION
//*****************************************************************************
void main() {
	//
	// Board Initialization
	//
	BoardInit();

	//
	// configure the GPIO pins for LEDs,UART
	//
	PinMuxConfig();

	//
	// Configure the UART
	//
#ifndef NOTERM
	InitTerm();
#endif  //NOTERM
	//
	// Display Application Banner
	//
	DisplayBanner(APPLICATION_NAME);

	//
	// Configure all 3 LEDs
	//
	GPIO_IF_LedConfigure(LED1 | LED2 | LED3);

	// switch off all LEDs
	GPIO_IF_LedOff(MCU_ALL_LED_IND);

	FileTest();

	/*//
	 // Start the SimpleLink Host
	 //
	 VStartSimpleLinkSpawnTask(SPAWN_TASK_PRIORITY);

	 //
	 // Start the WlanStationMode task
	 //
	 osi_TaskCreate( WlanStationMode, (const signed char*)"Wlan Station Task",
	 OSI_STACK_SIZE, NULL, 1, NULL );
	 //
	 // Start the task scheduler
	 //
	 osi_start();*/
}
Exemple #7
0
int main()
{

    // for timing how long tests take
    {
        boost::progress_timer timer;

        CoreFSTest();
        MakeKnoxCryptTest();
        FileDeviceTest();
        FileBlockTest();
        FileBlockIteratorTest();
        FileTest();
        ContentFolderTest();
    }

    simpletest::showResults();
}
Exemple #8
0
static void Handle_FileTest
(
    le_msg_MessageRef_t _msgRef

)
{
    // Get the message buffer pointer
    uint8_t* _msgBufPtr = ((_Message_t*)le_msg_GetPayloadPtr(_msgRef))->buffer;

    // Needed if we are returning a result or output values
    uint8_t* _msgBufStartPtr = _msgBufPtr;

    // Unpack the input parameters from the message
    int dataFile;
    dataFile = le_msg_GetFd(_msgRef);


    // Define storage for output parameters
    int dataOut;

    // Call the function
    FileTest ( dataFile, &dataOut );


    // Re-use the message buffer for the response
    _msgBufPtr = _msgBufStartPtr;


    // Pack any "out" parameters
    le_msg_SetFd(_msgRef, dataOut);

    // Return the response
    LE_DEBUG("Sending response to client session %p : %ti bytes sent",
             le_msg_GetSession(_msgRef),
             _msgBufPtr-_msgBufStartPtr);
    le_msg_Respond(_msgRef);
}
Exemple #9
0
void test1(void)
{
    uint32_t value=10;
    uint32_t data[] = {1, 2, 3, 4};
    size_t length=10;
    uint32_t output[length];
    char response[21];
    char more[21];

    allParameters(COMMON_TWO,
                  &value,
                  data,
                  4,
                  output,
                  &length,
                  "input string",
                  response,
                  sizeof(response),
                  more,
                  sizeof(more));

    LE_PRINT_VALUE("%i", value);
    LE_PRINT_ARRAY("%i", length, output);
    LE_PRINT_VALUE("%s", response);
    LE_PRINT_VALUE("%s", more);

    // Call again with a special value, so that nothing is returned for the 'output', 'response'
    // and 'more' output parameters. This could happen in a typical function, if an error is 
    // detected.
    
    // Make 'length' larger than actually defined for the 'output' parameter to verify that
    // only the maximum defined value is used on the server.
    length = 20;
    allParameters(COMMON_ZERO,
                  &value,
                  data,
                  4,
                  output,
                  &length,
                  "new string",
                  response,
                  sizeof(response),
                  more,
                  sizeof(more));

    LE_PRINT_VALUE("%i", value);
    LE_PRINT_ARRAY("%i", length, output);
    LE_PRINT_VALUE("%s", response);
    LE_PRINT_VALUE("%s", more);

    // Test file descriptors
    int fdToServer;
    int fdFromServer;

    // Open a file known to exist
    fdToServer = open("/usr/include/stdio.h", O_RDONLY);

    LE_PRINT_VALUE("%i", fdToServer);
    FileTest(fdToServer, &fdFromServer);
    LE_PRINT_VALUE("%i", fdFromServer);

    // Read and print out whatever is read from the server fd
    writeFdToLog(fdFromServer);
}
int ChimeraPintailCommand::execute(){
	try{
		
		if (abort == true) { if (calledHelp) { return 0; }  return 2;	}
		
		for (int s = 0; s < fastaFileNames.size(); s++) {
				
			m->mothurOut("Checking sequences from " + fastaFileNames[s] + " ..." ); m->mothurOutEndLine();

			int start = time(NULL);	
			
			//set user options
			if (maskfile == "default") { m->mothurOut("I am using the default 236627 EU009184.1 Shigella dysenteriae str. FBD013."); m->mothurOutEndLine();  }
			
			//check for quantile to save the time
			string baseName = templatefile;
			if (templatefile == "saved") { baseName = rdb->getSavedReference(); }
			
			string tempQuan = "";
			if ((!filter) && (maskfile == "")) {
				tempQuan = inputDir + m->getRootName(m->getSimpleName(baseName)) + "pintail.quan";
			}else if ((!filter) && (maskfile != "")) { 
				tempQuan = inputDir + m->getRootName(m->getSimpleName(baseName)) + "pintail.masked.quan";
			}else if ((filter) && (maskfile != "")) { 
				tempQuan = inputDir + m->getRootName(m->getSimpleName(baseName)) + "pintail.filtered." + m->getSimpleName(m->getRootName(fastaFileNames[s])) + "masked.quan";
			}else if ((filter) && (maskfile == "")) { 
				tempQuan = inputDir + m->getRootName(m->getSimpleName(baseName)) + "pintail.filtered." + m->getSimpleName(m->getRootName(fastaFileNames[s])) + "quan";
			}
			
			ifstream FileTest(tempQuan.c_str());
			if(FileTest){	
				bool GoodFile = m->checkReleaseVersion(FileTest, m->getVersion());
				if (GoodFile) {  
					m->mothurOut("I found " + tempQuan + " in your input file directory. I will use it to save time."); m->mothurOutEndLine();  quanfile = tempQuan;  FileTest.close();	
				}
			}else {
				string tryPath = m->getDefaultPath();
				string tempQuan = "";
				if ((!filter) && (maskfile == "")) {
					tempQuan = tryPath + m->getRootName(m->getSimpleName(baseName)) + "pintail.quan";
				}else if ((!filter) && (maskfile != "")) { 
					tempQuan = tryPath + m->getRootName(m->getSimpleName(baseName)) + "pintail.masked.quan";
				}else if ((filter) && (maskfile != "")) { 
					tempQuan = tryPath + m->getRootName(m->getSimpleName(baseName)) + "pintail.filtered." + m->getSimpleName(m->getRootName(fastaFileNames[s])) + "masked.quan";
				}else if ((filter) && (maskfile == "")) { 
					tempQuan = tryPath + m->getRootName(m->getSimpleName(baseName)) + "pintail.filtered." + m->getSimpleName(m->getRootName(fastaFileNames[s])) + "quan";
				}
				
				ifstream FileTest2(tempQuan.c_str());
				if(FileTest2){	
					bool GoodFile = m->checkReleaseVersion(FileTest2, m->getVersion());
					if (GoodFile) {  
						m->mothurOut("I found " + tempQuan + " in your input file directory. I will use it to save time."); m->mothurOutEndLine();  quanfile = tempQuan;  FileTest2.close();	
					}
				}
			}
			chimera = new Pintail(fastaFileNames[s], templatefile, filter, processors, maskfile, consfile, quanfile, window, increment, outputDir);
			
			if (outputDir == "") { outputDir = m->hasPath(fastaFileNames[s]);  }//if user entered a file with a path then preserve it
			string outputFileName, accnosFileName;
            map<string, string> variables;
            variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s]));
			if (maskfile != "") { variables["[tag]"] = m->getSimpleName(m->getRootName(maskfile)); }
            outputFileName = getOutputFileName("chimera", variables);
            accnosFileName = getOutputFileName("accnos", variables);
			
			
			if (m->control_pressed) { delete chimera; for (int j = 0; j < outputNames.size(); j++) {	m->mothurRemove(outputNames[j]);	}  return 0;	}
			
			if (chimera->getUnaligned()) { 
				m->mothurOut("Your template sequences are different lengths, please correct."); m->mothurOutEndLine(); 
				delete chimera;
				return 0; 
			}
			templateSeqsLength = chimera->getLength();
		
		#ifdef USE_MPI
			int pid, numSeqsPerProcessor; 
				int tag = 2001;
				vector<unsigned long long> MPIPos;
				
				MPI_Status status; 
				MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
				MPI_Comm_size(MPI_COMM_WORLD, &processors); 

				MPI_File inMPI;
				MPI_File outMPI;
				MPI_File outMPIAccnos;
				
				int outMode=MPI_MODE_CREATE|MPI_MODE_WRONLY; 
				int inMode=MPI_MODE_RDONLY; 
				
				char outFilename[1024];
				strcpy(outFilename, outputFileName.c_str());
				
				char outAccnosFilename[1024];
				strcpy(outAccnosFilename, accnosFileName.c_str());
				
				char inFileName[1024];
				strcpy(inFileName, fastaFileNames[s].c_str());

				MPI_File_open(MPI_COMM_WORLD, inFileName, inMode, MPI_INFO_NULL, &inMPI);  //comm, filename, mode, info, filepointer
				MPI_File_open(MPI_COMM_WORLD, outFilename, outMode, MPI_INFO_NULL, &outMPI);
				MPI_File_open(MPI_COMM_WORLD, outAccnosFilename, outMode, MPI_INFO_NULL, &outMPIAccnos);
				
				if (m->control_pressed) { outputTypes.clear();  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos);  for (int j = 0; j < outputNames.size(); j++) {	m->mothurRemove(outputNames[j]);	}  delete chimera; return 0;  }

				if (pid == 0) { //you are the root process 
								
					MPIPos = m->setFilePosFasta(fastaFileNames[s], numSeqs); //fills MPIPos, returns numSeqs
					
					//send file positions to all processes
					for(int i = 1; i < processors; i++) { 
						MPI_Send(&numSeqs, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
						MPI_Send(&MPIPos[0], (numSeqs+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
					}
					
					//figure out how many sequences you have to align
					numSeqsPerProcessor = numSeqs / processors;
					int startIndex =  pid * numSeqsPerProcessor;
					if(pid == (processors - 1)){	numSeqsPerProcessor = numSeqs - pid * numSeqsPerProcessor; 	}
				
					//do your part
					driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPI, outMPIAccnos, MPIPos);
					
					if (m->control_pressed) { outputTypes.clear();  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos);  m->mothurRemove(outputFileName);  m->mothurRemove(accnosFileName);  for (int j = 0; j < outputNames.size(); j++) {	m->mothurRemove(outputNames[j]);	}  delete chimera; return 0;  }
					
				}else{ //you are a child process
					MPI_Recv(&numSeqs, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
					MPIPos.resize(numSeqs+1);
					MPI_Recv(&MPIPos[0], (numSeqs+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
					
					//figure out how many sequences you have to align
					numSeqsPerProcessor = numSeqs / processors;
					int startIndex =  pid * numSeqsPerProcessor;
					if(pid == (processors - 1)){	numSeqsPerProcessor = numSeqs - pid * numSeqsPerProcessor; 	}
					
					//do your part
					driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPI, outMPIAccnos, MPIPos);
					
					if (m->control_pressed) { outputTypes.clear();  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos);  for (int j = 0; j < outputNames.size(); j++) {	m->mothurRemove(outputNames[j]);	}  delete chimera; return 0;  }
				}
				
				//close files 
				MPI_File_close(&inMPI);
				MPI_File_close(&outMPI);
				MPI_File_close(&outMPIAccnos);
				MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
		#else
						
			//break up file
			#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
				vector<unsigned long long> positions = m->divideFile(fastaFileNames[s], processors);
			
				for (int i = 0; i < (positions.size()-1); i++) {
					lines.push_back(new linePair(positions[i], positions[(i+1)]));
				}	
			
				if(processors == 1){
		
					numSeqs = driver(lines[0], outputFileName, fastaFileNames[s], accnosFileName);
					
					if (m->control_pressed) { outputTypes.clear(); m->mothurRemove(outputFileName); m->mothurRemove(accnosFileName); for (int j = 0; j < outputNames.size(); j++) {	m->mothurRemove(outputNames[j]);	} for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear(); delete chimera; return 0; }
					
				}else{
					processIDS.resize(0);
					
					numSeqs = createProcesses(outputFileName, fastaFileNames[s], accnosFileName); 
				
					rename((outputFileName + toString(processIDS[0]) + ".temp").c_str(), outputFileName.c_str());
					rename((accnosFileName + toString(processIDS[0]) + ".temp").c_str(), accnosFileName.c_str());
						
					//append output files
					for(int i=1;i<processors;i++){
						m->appendFiles((outputFileName + toString(processIDS[i]) + ".temp"), outputFileName);
						m->mothurRemove((outputFileName + toString(processIDS[i]) + ".temp"));
					}
					
					//append output files
					for(int i=1;i<processors;i++){
						m->appendFiles((accnosFileName + toString(processIDS[i]) + ".temp"), accnosFileName);
						m->mothurRemove((accnosFileName + toString(processIDS[i]) + ".temp"));
					}
										
					if (m->control_pressed) { 
						m->mothurRemove(outputFileName); 
						m->mothurRemove(accnosFileName);
						for (int j = 0; j < outputNames.size(); j++) {	m->mothurRemove(outputNames[j]);	} outputTypes.clear();
						for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear();
						delete chimera;
						return 0;
					}
				}

			#else
				lines.push_back(new linePair(0, 1000));
				numSeqs = driver(lines[0], outputFileName, fastaFileNames[s], accnosFileName);
				
				if (m->control_pressed) { outputTypes.clear(); m->mothurRemove(outputFileName); m->mothurRemove(accnosFileName); for (int j = 0; j < outputNames.size(); j++) {	m->mothurRemove(outputNames[j]);	} for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear(); delete chimera; return 0; }
			#endif
			
		#endif	
		
			delete chimera;
			for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear();
			
			outputNames.push_back(outputFileName); outputTypes["chimera"].push_back(outputFileName);
			outputNames.push_back(accnosFileName); outputTypes["accnos"].push_back(accnosFileName);
			
			m->mothurOutEndLine();
			m->mothurOutEndLine(); m->mothurOut("It took " + toString(time(NULL) - start) + " secs to check " + toString(numSeqs) + " sequences.");	m->mothurOutEndLine();
		}
		
		//set accnos file as new current accnosfile
		string current = "";
		itTypes = outputTypes.find("accnos");
		if (itTypes != outputTypes.end()) {
			if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setAccnosFile(current); }
		}
		
		m->mothurOutEndLine();
		m->mothurOut("Output File Names: "); m->mothurOutEndLine();
		for (int i = 0; i < outputNames.size(); i++) {	m->mothurOut(outputNames[i]); m->mothurOutEndLine();	}	
		m->mothurOutEndLine();
			
		return 0;
		
	}
	catch(exception& e) {
		m->errorOut(e, "ChimeraPintailCommand", "execute");
		exit(1);
	}
}
//***************************************************************************************************************
ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
	try {
		abort = false; calledHelp = false;   
		rdb = ReferenceDB::getInstance();
		
		//allow user to run help
		if(option == "help") { help(); abort = true; calledHelp = true; }
		else if(option == "citation") { citation(); abort = true; calledHelp = true;}
		
		else {
			vector<string> myArray = setParameters();
			
			OptionParser parser(option);
			map<string,string> parameters = parser.getParameters();
			
			ValidParameters validParameter("chimera.pintail");
			map<string,string>::iterator it;
			
			//check to make sure all parameters are valid for command
			for (it = parameters.begin(); it != parameters.end(); it++) { 
				if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
			}
			
			vector<string> tempOutNames;
			outputTypes["chimera"] = tempOutNames;
			outputTypes["accnos"] = tempOutNames;
        
		
			//if the user changes the input directory command factory will send this info to us in the output parameter 
			inputDir = validParameter.validFile(parameters, "inputdir", false);		
			if (inputDir == "not found"){	inputDir = "";		}
			else {
				string path;
				it = parameters.find("reference");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["reference"] = inputDir + it->second;		}
				}
				
				it = parameters.find("conservation");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["conservation"] = inputDir + it->second;		}
				}
				
				it = parameters.find("quantile");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["quantile"] = inputDir + it->second;		}
				}
			}

			
			//check for required parameters
			fastafile = validParameter.validFile(parameters, "fasta", false);
			if (fastafile == "not found") { 				
				//if there is a current fasta file, use it
				string filename = m->getFastaFile(); 
				if (filename != "") { fastaFileNames.push_back(filename); m->mothurOut("Using " + filename + " as input file for the fasta parameter."); m->mothurOutEndLine(); }
				else { 	m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; }
			}else { 
				m->splitAtDash(fastafile, fastaFileNames);
				
				//go through files and make sure they are good, if not, then disregard them
				for (int i = 0; i < fastaFileNames.size(); i++) {
					
					bool ignore = false;
					if (fastaFileNames[i] == "current") { 
						fastaFileNames[i] = m->getFastaFile(); 
						if (fastaFileNames[i] != "") {  m->mothurOut("Using " + fastaFileNames[i] + " as input file for the fasta parameter where you had given current."); m->mothurOutEndLine(); }
						else { 	
							m->mothurOut("You have no current fastafile, ignoring current."); m->mothurOutEndLine(); ignore=true; 
							//erase from file list
							fastaFileNames.erase(fastaFileNames.begin()+i);
							i--;
						}
					}
					
					if (!ignore) {
					
						if (inputDir != "") {
							string path = m->hasPath(fastaFileNames[i]);
							//if the user has not given a path then, add inputdir. else leave path alone.
							if (path == "") {	fastaFileNames[i] = inputDir + fastaFileNames[i];		}
						}
		
						int ableToOpen;
						ifstream in;
						
						ableToOpen = m->openInputFile(fastaFileNames[i], in, "noerror");
					
						//if you can't open it, try default location
						if (ableToOpen == 1) {
							if (m->getDefaultPath() != "") { //default path is set
								string tryPath = m->getDefaultPath() + m->getSimpleName(fastaFileNames[i]);
								m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
								ifstream in2;
								ableToOpen = m->openInputFile(tryPath, in2, "noerror");
								in2.close();
								fastaFileNames[i] = tryPath;
							}
						}
						
						if (ableToOpen == 1) {
							if (m->getOutputDir() != "") { //default path is set
								string tryPath = m->getOutputDir() + m->getSimpleName(fastaFileNames[i]);
								m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
								ifstream in2;
								ableToOpen = m->openInputFile(tryPath, in2, "noerror");
								in2.close();
								fastaFileNames[i] = tryPath;
							}
						}

						in.close();
						
						if (ableToOpen == 1) { 
							m->mothurOut("Unable to open " + fastaFileNames[i] + ". It will be disregarded."); m->mothurOutEndLine(); 
							//erase from file list
							fastaFileNames.erase(fastaFileNames.begin()+i);
							i--;
						}else {
							m->setFastaFile(fastaFileNames[i]);
						}
					}
				}
				
				//make sure there is at least one valid file left
				if (fastaFileNames.size() == 0) { m->mothurOut("no valid files."); m->mothurOutEndLine(); abort = true; }
			}
			
			string temp;
			temp = validParameter.validFile(parameters, "filter", false);			if (temp == "not found") { temp = "F"; }
			filter = m->isTrue(temp);
			
			temp = validParameter.validFile(parameters, "processors", false);	if (temp == "not found"){	temp = m->getProcessors();	}
			m->setProcessors(temp);
			m->mothurConvert(temp, processors);
			
			temp = validParameter.validFile(parameters, "window", false);			if (temp == "not found") { temp = "0"; }
			m->mothurConvert(temp, window);
			
			temp = validParameter.validFile(parameters, "increment", false);		if (temp == "not found") { temp = "25"; }
			m->mothurConvert(temp, increment);
			
			temp = validParameter.validFile(parameters, "save", false);			if (temp == "not found"){	temp = "f";				}
			save = m->isTrue(temp); 
			rdb->save = save; 
			if (save) { //clear out old references
				rdb->clearMemory();	
			}
			
			//this has to go after save so that if the user sets save=t and provides no reference we abort
			templatefile = validParameter.validFile(parameters, "reference", true);
			if (templatefile == "not found") { 
				//check for saved reference sequences
				if (rdb->referenceSeqs.size() != 0) {
					templatefile = "saved";
				}else {
					m->mothurOut("[ERROR]: You don't have any saved reference sequences and the reference parameter is a required."); 
					m->mothurOutEndLine();
					abort = true; 
				}
			}else if (templatefile == "not open") { abort = true; }	
			else {	if (save) {	rdb->setSavedReference(templatefile);	}	}
			
			
			maskfile = validParameter.validFile(parameters, "mask", false);
			if (maskfile == "not found") { maskfile = "";  }	
			else if (maskfile != "default")  { 
				if (inputDir != "") {
					string path = m->hasPath(maskfile);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	maskfile = inputDir + maskfile;		}
				}

				ifstream in;
				int	ableToOpen = m->openInputFile(maskfile, in, "no error");
				if (ableToOpen == 1) { 
					if (m->getDefaultPath() != "") { //default path is set
							string tryPath = m->getDefaultPath() + m->getSimpleName(maskfile);
							m->mothurOut("Unable to open " + maskfile + ". Trying default " + tryPath); m->mothurOutEndLine();
							ifstream in2;
							ableToOpen = m->openInputFile(tryPath, in2, "noerror");
							in2.close();
							maskfile = tryPath;
					}
				}
				
				if (ableToOpen == 1) {
						if (m->getOutputDir() != "") { //default path is set
							string tryPath = m->getOutputDir() + m->getSimpleName(maskfile);
							m->mothurOut("Unable to open " + maskfile + ". Trying output directory " + tryPath); m->mothurOutEndLine();
							ifstream in2;
							ableToOpen = m->openInputFile(tryPath, in2, "noerror");
							in2.close();
							maskfile = tryPath;
						}
				}
				
				in.close();
					
				if (ableToOpen == 1) { 
						m->mothurOut("Unable to open " + maskfile + "."); m->mothurOutEndLine(); 
						abort = true;
				}
			}

			
			//if the user changes the output directory command factory will send this info to us in the output parameter 
			outputDir = validParameter.validFile(parameters, "outputdir", false);		if (outputDir == "not found"){	outputDir = "";	}
		
			consfile = validParameter.validFile(parameters, "conservation", true);
			if (consfile == "not open") { abort = true; }
			else if (consfile == "not found") { 
				consfile = "";  
				//check for consfile
				string tempConsFile = m->getRootName(inputDir + m->getSimpleName(templatefile)) + "freq";
				ifstream FileTest(tempConsFile.c_str());
				if(FileTest){	
					bool GoodFile = m->checkReleaseVersion(FileTest, m->getVersion());
					if (GoodFile) {  
						m->mothurOut("I found " + tempConsFile + " in your input file directory. I will use it to save time."); m->mothurOutEndLine();  consfile = tempConsFile;  FileTest.close();	
					}
				}else {
					string tempConsFile = m->getDefaultPath() + m->getRootName(m->getSimpleName(templatefile)) + "freq";
					ifstream FileTest2(tempConsFile.c_str());
					if(FileTest2){	
						bool GoodFile = m->checkReleaseVersion(FileTest2, m->getVersion());
						if (GoodFile) {  
							m->mothurOut("I found " + tempConsFile + " in your input file directory. I will use it to save time."); m->mothurOutEndLine();  consfile = tempConsFile;  FileTest2.close();	
						}
					}
				}
			}	
			
			quanfile = validParameter.validFile(parameters, "quantile", true);
			if (quanfile == "not open") { abort = true; }
			else if (quanfile == "not found") { quanfile = ""; }
		}
	}
	catch(exception& e) {
		m->errorOut(e, "ChimeraPintailCommand", "ChimeraPintailCommand");
		exit(1);
	}
}