int insert_regions(region_t **regions, int num_regions, region_table_t *table) {
int rc;
sqlite3_stmt *stmt = table->insert_region_stmt;
sqlite3* db = table->storage;
char *sql_begin = "BEGIN TRANSACTION";
rc = exec_sql(sql_begin, db);
if (rc != SQLITE_OK) {
LOG_ERROR_F("Could not insert regions: %s (%d)\n",
sqlite3_errmsg(db), sqlite3_errcode(db));
return rc;
}
for (int i = 0; i < num_regions; i++) {
region_t *region = regions[i];
sqlite3_bind_text(stmt, 1, region->chromosome, strlen(region->chromosome), SQLITE_STATIC);
sqlite3_bind_int64(stmt, 2, region->start_position);
sqlite3_bind_int64(stmt, 3, region->end_position);
if (region->strand) {
sqlite3_bind_text(stmt, 4, region->strand, strlen(region->strand), SQLITE_STATIC);
} else {
sqlite3_bind_null(stmt, 4);
}
if (region->type) {
sqlite3_bind_text(stmt, 5, region->type, strlen(region->type), SQLITE_STATIC);
} else {
sqlite3_bind_null(stmt, 5);
}
if ((rc = sqlite3_step(stmt) == SQLITE_DONE)) {
// Update value in chunks hashtable
update_chunks_hash(region->chromosome, UINT_MAX, REGIONS_CHUNKSIZE,
region->start_position, region->end_position, table->chunks);
} else {
LOG_ERROR_F("Could not insert region %s:%ld-%ld: %s (%d)\n",
region->chromosome, region->start_position, region->end_position,
sqlite3_errmsg(db), sqlite3_errcode(db));
}
sqlite3_reset(stmt);
}
char *sql_end = "END TRANSACTION";
rc = exec_sql(sql_end, db);
if (rc != SQLITE_OK) {
LOG_ERROR_F("Could not insert regions: %s (%d)\n",
sqlite3_errmsg(db), sqlite3_errcode(db));
return rc;
}
return rc;
}
int remove_exact_region(region_t *region, region_table_t *table) {
int rc;
sqlite3_stmt *stmt1 = table->remove_exact_region_stmt, *stmt2;
sqlite3* db = table->storage;
char *sql_begin = "BEGIN TRANSACTION";
rc = exec_sql(sql_begin, db);
if (rc != SQLITE_OK) {
LOG_ERROR_F("Could not remove region: %s (%d)\n",
sqlite3_errmsg(db), sqlite3_errcode(db));
return rc;
}
sqlite3_bind_text(stmt1, 1, region->chromosome, strlen(region->chromosome), SQLITE_STATIC);
sqlite3_bind_int64(stmt1, 2, region->start_position);
sqlite3_bind_int64(stmt1, 3, region->end_position);
if ((rc = sqlite3_step(stmt1)) == SQLITE_DONE) {
sqlite3_reset(stmt1);
int chunk_start = region->start_position / REGIONS_CHUNKSIZE;
int chunk_end = region->end_position / REGIONS_CHUNKSIZE;
// Decrement features_count in chunk table
char sql_chunks[] = "UPDATE chunk SET features_count = features_count - 1 WHERE chromosome = ?1 AND \
(chunk_id >= ?2 AND chunk_id <= ?3)";
rc = sqlite3_prepare_v2(db, sql_chunks, strlen(sql_chunks), &stmt2, NULL);
if (rc != SQLITE_OK) {
LOG_ERROR_F("Could not remove region %s:%ld-%ld: %s (%d)\n",
region->chromosome, region->start_position, region->end_position,
sqlite3_errmsg(db), sqlite3_errcode(db));
return rc;
}
sqlite3_bind_text(stmt2, 1, region->chromosome, strlen(region->chromosome), SQLITE_STATIC);
sqlite3_bind_int64(stmt2, 2, chunk_start);
sqlite3_bind_int64(stmt2, 3, chunk_end);
if ((rc = sqlite3_step(stmt2)) != SQLITE_DONE) {
LOG_ERROR_F("Could not remove region %s:%ld-%ld: %s (%d)\n",
region->chromosome, region->start_position, region->end_position,
sqlite3_errmsg(db), sqlite3_errcode(db));
return rc;
}
sqlite3_finalize(stmt2);
} else {
inline int exec_sql(char *sql, sqlite3* db) {
int rc;
char *error_msg;
if (rc = sqlite3_exec(db, sql, NULL, NULL, &error_msg)) {
LOG_ERROR_F("Stats database failed (%s): %s\n", sql, error_msg);
}
return rc;
}
int create_vcf_index(sqlite3 *db) {
int rc;
char sql[128];
char *error_msg;
// create chunks index
sprintf(sql, "CREATE INDEX record_query_fields_chromosome_start_end_idx ON chunk (chromosome, start, end)");
if (rc = sqlite3_exec(db, sql, NULL, NULL, &error_msg)) {
LOG_ERROR_F("Stats database failed creating VCF index: %s\n", error_msg);
}
return rc;
}
int read_stats_configuration(const char *filename, stats_options_t *options, shared_options_t *shared_options) {
if (filename == NULL || options == NULL || shared_options == NULL) {
return -1;
}
config_t *config = (config_t*) calloc (1, sizeof(config_t));
int ret_code = config_read_file(config, filename);
if (ret_code == CONFIG_FALSE) {
LOG_ERROR_F("config file error: %s\n", config_error_text(config));
return ret_code;
}
// Read number of threads that will make request to the web service
ret_code = config_lookup_int(config, "vcf-tools.stats.num-threads", shared_options->num_threads->ival);
if (ret_code == CONFIG_FALSE) {
LOG_WARN("Number of threads not found in config file, must be set via command-line\n");
} else {
LOG_DEBUG_F("num-threads = %ld\n", *(shared_options->num_threads->ival));
}
// Read maximum number of batches that can be stored at certain moment
ret_code = config_lookup_int(config, "vcf-tools.stats.max-batches", shared_options->max_batches->ival);
if (ret_code == CONFIG_FALSE) {
LOG_WARN("Maximum number of batches not found in configuration file, must be set via command-line\n");
} else {
LOG_DEBUG_F("max-batches = %ld\n", *(shared_options->max_batches->ival));
}
// Read size of every batch read
ret_code = config_lookup_int(config, "vcf-tools.stats.batch-lines", shared_options->batch_lines->ival);
ret_code |= config_lookup_int(config, "vcf-tools.stats.batch-bytes", shared_options->batch_bytes->ival);
if (ret_code == CONFIG_FALSE) {
LOG_WARN("Neither batch lines nor bytes found in configuration file, must be set via command-line\n");
}
/*else {
LOG_DEBUG_F("batch-lines = %ld\n", *(shared_options->batch_size->ival));
}*/
config_destroy(config);
free(config);
return 0;
}
int read_tdt_configuration(const char *filename, tdt_options_t *tdt_options, shared_options_t *shared_options) {
if (filename == NULL || tdt_options == NULL || shared_options == NULL) {
return -1;
}
config_t *config = (config_t*) calloc (1, sizeof(config_t));
int ret_code = config_read_file(config, filename);
if (ret_code == CONFIG_FALSE) {
LOG_ERROR_F("Configuration file error: %s\n", config_error_text(config));
return CANT_READ_CONFIG_FILE;
}
const char *tmp_string;
// Read number of threads that will make request to the web service
ret_code = config_lookup_int(config, "gwas.tdt.num-threads", shared_options->num_threads->ival);
if (ret_code == CONFIG_FALSE) {
LOG_WARN("Number of threads not found in config file, must be set via command-line");
} else {
LOG_DEBUG_F("num-threads = %ld\n", *(shared_options->num_threads->ival));
}
// Read maximum number of batches that can be stored at certain moment
ret_code = config_lookup_int(config, "gwas.tdt.max-batches", shared_options->max_batches->ival);
if (ret_code == CONFIG_FALSE) {
LOG_WARN("Maximum number of batches not found in configuration file, must be set via command-line");
} else {
LOG_DEBUG_F("max-batches = %ld\n", *(shared_options->max_batches->ival));
}
// Read size of a batch (in lines or bytes)
ret_code = config_lookup_int(config, "gwas.tdt.batch-lines", shared_options->batch_lines->ival);
ret_code |= config_lookup_int(config, "gwas.tdt.batch-bytes", shared_options->batch_bytes->ival);
if (ret_code == CONFIG_FALSE) {
LOG_WARN("Neither batch lines nor bytes found in configuration file, must be set via command-line");
}
config_destroy(config);
free(config);
return 0;
}
int find_region_by_type(region_t *region, region_table_t *table) {
assert(region);
assert(region->type);
assert(table);
if (!table->is_ready) { // Don't allow queries over an un-indexed DB
finish_region_table_loading(table);
}
int count = 0;
sqlite3* db = table->storage;
sqlite3_stmt *query_stmt = table->find_region_type_stmt;
sqlite3_bind_text(query_stmt, 1, region->chromosome, strlen(region->chromosome), SQLITE_STATIC);
sqlite3_bind_int64(query_stmt, 2, region->start_position);
sqlite3_bind_int64(query_stmt, 3, region->end_position);
sqlite3_bind_text(query_stmt, 4, region->type, strlen(region->type), SQLITE_STATIC);
if (sqlite3_step(query_stmt) == SQLITE_ROW) {
count = sqlite3_column_int(query_stmt, 0);
} else {
// Retry once
sqlite3_reset(query_stmt);
sqlite3_bind_text(query_stmt, 1, region->chromosome, strlen(region->chromosome), SQLITE_STATIC);
sqlite3_bind_int64(query_stmt, 2, region->start_position);
sqlite3_bind_int64(query_stmt, 3, region->end_position);
sqlite3_bind_text(query_stmt, 4, region->type, strlen(region->type), SQLITE_STATIC);
if (sqlite3_step(query_stmt) == SQLITE_ROW) {
count = sqlite3_column_int(query_stmt, 0);
} else {
LOG_ERROR_F("Regions table failed: %s (%d)\n", sqlite3_errmsg(db), sqlite3_errcode(db));
}
}
sqlite3_reset(query_stmt);
return count > 0;
}
int ped_ragel_read(list_t *batches_list, size_t batch_size, ped_file_t *file)
{
int cs;
char *p = file->data;
char *pe = p + file->data_len;
char *eof = pe;
char *ts;
int custom_field_count = 0;
current_batch = ped_batch_new(batch_size);
#line 41 "ped_reader.c"
{
cs = ped_start;
}
#line 46 "ped_reader.c"
{
if ( p == pe )
goto _test_eof;
switch ( cs )
{
case 21:
switch( (*p) ) {
case 10: goto st22;
case 35: goto st16;
}
if ( 33 <= (*p) && (*p) <= 126 )
goto tr36;
goto tr0;
tr0:
#line 53 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'family' field\n", lines + 1, file->filename);
}
goto st0;
tr3:
#line 65 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'individual' field\n", lines + 1, file->filename);
}
goto st0;
tr7:
#line 77 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'father' field\n", lines + 1, file->filename);
}
goto st0;
tr11:
#line 89 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'mother' field\n", lines + 1, file->filename);
}
goto st0;
tr15:
#line 109 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'sex' field\n", lines + 1, file->filename);
}
goto st0;
tr19:
#line 124 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'phenotype' field\n", lines + 1, file->filename);
}
goto st0;
tr26:
#line 141 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'header' field\n", lines + 1, file->filename);
}
goto st0;
tr44:
#line 161 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in '%s' field\n", lines + 1, file->filename, current_record->custom_field);
}
goto st0;
#line 108 "ped_reader.c"
st0:
cs = 0;
goto _out;
st22:
if ( ++p == pe )
goto _test_eof22;
case 22:
if ( (*p) == 10 )
goto st22;
goto st0;
tr36:
#line 22 "ped.ragel"
{
current_record = create_ped_record();
genotype = 0;
}
#line 45 "ped.ragel"
{
ts = p;
}
goto st1;
st1:
if ( ++p == pe )
goto _test_eof1;
case 1:
#line 134 "ped_reader.c"
if ( (*p) == 9 )
goto tr1;
if ( 33 <= (*p) && (*p) <= 126 )
goto st1;
goto tr0;
tr1:
#line 49 "ped.ragel"
{
set_ped_record_family_id(strndup(ts, p-ts), current_record);
}
goto st2;
st2:
if ( ++p == pe )
goto _test_eof2;
case 2:
#line 150 "ped_reader.c"
if ( (*p) == 95 )
goto tr4;
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto tr4;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto tr4;
} else
goto tr4;
goto tr3;
tr4:
#line 57 "ped.ragel"
{
ts = p;
}
goto st3;
st3:
if ( ++p == pe )
goto _test_eof3;
case 3:
#line 172 "ped_reader.c"
switch( (*p) ) {
case 9: goto tr5;
case 95: goto st3;
}
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto st3;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto st3;
} else
goto st3;
goto tr3;
tr5:
#line 61 "ped.ragel"
{
set_ped_record_individual_id(strndup(ts, p-ts), current_record);
}
goto st4;
st4:
if ( ++p == pe )
goto _test_eof4;
case 4:
#line 196 "ped_reader.c"
switch( (*p) ) {
case 46: goto tr8;
case 95: goto tr9;
}
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto tr9;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto tr9;
} else
goto tr9;
goto tr7;
tr8:
#line 69 "ped.ragel"
{
ts = p;
}
goto st5;
st5:
if ( ++p == pe )
goto _test_eof5;
case 5:
#line 220 "ped_reader.c"
if ( (*p) == 9 )
goto tr10;
goto tr7;
tr10:
#line 73 "ped.ragel"
{
set_ped_record_father_id(strndup(ts, p-ts), current_record);
}
goto st6;
st6:
if ( ++p == pe )
goto _test_eof6;
case 6:
#line 234 "ped_reader.c"
switch( (*p) ) {
case 46: goto tr12;
case 95: goto tr13;
}
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto tr13;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto tr13;
} else
goto tr13;
goto tr11;
tr12:
#line 81 "ped.ragel"
{
ts = p;
}
goto st7;
st7:
if ( ++p == pe )
goto _test_eof7;
case 7:
#line 258 "ped_reader.c"
if ( (*p) == 9 )
goto tr14;
goto tr11;
tr14:
#line 85 "ped.ragel"
{
set_ped_record_mother_id(strndup(ts, p-ts), current_record);
}
goto st8;
st8:
if ( ++p == pe )
goto _test_eof8;
case 8:
#line 272 "ped_reader.c"
if ( (*p) == 46 )
goto tr16;
if ( 48 <= (*p) && (*p) <= 57 )
goto tr17;
goto tr15;
tr16:
#line 93 "ped.ragel"
{
ts = p;
}
goto st9;
st9:
if ( ++p == pe )
goto _test_eof9;
case 9:
#line 288 "ped_reader.c"
if ( (*p) == 9 )
goto tr18;
goto tr15;
tr18:
#line 97 "ped.ragel"
{
char *field = strndup(ts, p-ts);
enum Sex sex = UNKNOWN_SEX;
if (atoi(field) == 1) {
sex = MALE;
} else if (atoi(field) == 2) {
sex = FEMALE;
}
set_ped_record_sex(sex, current_record);
free(field); // Not set as ped_record_t variable -> not freed later
}
goto st10;
st10:
if ( ++p == pe )
goto _test_eof10;
case 10:
#line 310 "ped_reader.c"
switch( (*p) ) {
case 32: goto tr20;
case 95: goto tr20;
}
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto tr20;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto tr20;
} else
goto tr20;
goto tr19;
tr20:
#line 113 "ped.ragel"
{
ts = p;
}
goto st23;
tr42:
#line 117 "ped.ragel"
{
if (strncmp(".", ts, 1)) {
char *field = strndup(ts, p-ts);
set_ped_record_phenotype(field, current_record, file);
}
}
#line 145 "ped.ragel"
{
custom_field_count = 6;
}
goto st23;
st23:
if ( ++p == pe )
goto _test_eof23;
case 23:
#line 347 "ped_reader.c"
switch( (*p) ) {
case 9: goto tr39;
case 10: goto tr40;
case 32: goto tr42;
case 95: goto st23;
}
if ( (*p) < 48 ) {
if ( 11 <= (*p) && (*p) <= 13 )
goto tr41;
} else if ( (*p) > 57 ) {
if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto st23;
} else if ( (*p) >= 65 )
goto st23;
} else
goto st23;
goto tr19;
tr39:
#line 117 "ped.ragel"
{
if (strncmp(".", ts, 1)) {
char *field = strndup(ts, p-ts);
set_ped_record_phenotype(field, current_record, file);
}
}
#line 145 "ped.ragel"
{
custom_field_count = 6;
}
goto st24;
tr49:
#line 153 "ped.ragel"
{
char* field_name = strndup(ts, p-ts);
custom_field_count++;
if (custom_field_count == file->num_field) {
set_ped_record_custom_field(field_name, current_record, file);
}
}
goto st24;
st24:
if ( ++p == pe )
goto _test_eof24;
case 24:
#line 393 "ped_reader.c"
switch( (*p) ) {
case 9: goto st24;
case 10: goto tr46;
}
if ( (*p) > 13 ) {
if ( 32 <= (*p) && (*p) <= 126 )
goto tr48;
} else if ( (*p) >= 11 )
goto st26;
goto tr44;
tr40:
#line 117 "ped.ragel"
{
if (strncmp(".", ts, 1)) {
char *field = strndup(ts, p-ts);
set_ped_record_phenotype(field, current_record, file);
}
}
#line 145 "ped.ragel"
{
custom_field_count = 6;
}
#line 27 "ped.ragel"
{
// If batch is full, add to the list of batches and create a new, empty one
if (ped_batch_is_full(current_batch))
{
list_item_t *item = list_item_new(num_records, 1, current_batch);
list_insert_item(item, batches_list);
LOG_DEBUG_F("Batch added - %zu records\n", current_batch->length);
current_batch = ped_batch_new(batch_size);
}
// Add current record to current batch
if (current_record) {
add_record_to_ped_batch(current_record, current_batch);
num_records++;
}
current_record = NULL;
}
#line 18 "ped.ragel"
{
lines++;
}
goto st25;
tr46:
#line 27 "ped.ragel"
{
// If batch is full, add to the list of batches and create a new, empty one
if (ped_batch_is_full(current_batch))
{
list_item_t *item = list_item_new(num_records, 1, current_batch);
list_insert_item(item, batches_list);
LOG_DEBUG_F("Batch added - %zu records\n", current_batch->length);
current_batch = ped_batch_new(batch_size);
}
// Add current record to current batch
if (current_record) {
add_record_to_ped_batch(current_record, current_batch);
num_records++;
}
current_record = NULL;
}
#line 18 "ped.ragel"
{
lines++;
}
goto st25;
tr50:
#line 153 "ped.ragel"
{
char* field_name = strndup(ts, p-ts);
custom_field_count++;
if (custom_field_count == file->num_field) {
set_ped_record_custom_field(field_name, current_record, file);
}
}
#line 27 "ped.ragel"
{
// If batch is full, add to the list of batches and create a new, empty one
if (ped_batch_is_full(current_batch))
{
list_item_t *item = list_item_new(num_records, 1, current_batch);
list_insert_item(item, batches_list);
LOG_DEBUG_F("Batch added - %zu records\n", current_batch->length);
current_batch = ped_batch_new(batch_size);
}
// Add current record to current batch
if (current_record) {
add_record_to_ped_batch(current_record, current_batch);
num_records++;
}
current_record = NULL;
}
#line 18 "ped.ragel"
{
lines++;
}
goto st25;
st25:
if ( ++p == pe )
goto _test_eof25;
case 25:
#line 499 "ped_reader.c"
switch( (*p) ) {
case 10: goto tr46;
case 32: goto st26;
}
if ( (*p) < 33 ) {
if ( 9 <= (*p) && (*p) <= 13 )
goto st26;
} else if ( (*p) > 34 ) {
if ( 36 <= (*p) && (*p) <= 126 )
goto tr36;
} else
goto tr36;
goto tr0;
tr41:
#line 117 "ped.ragel"
{
if (strncmp(".", ts, 1)) {
char *field = strndup(ts, p-ts);
set_ped_record_phenotype(field, current_record, file);
}
}
#line 145 "ped.ragel"
{
custom_field_count = 6;
}
goto st26;
tr51:
#line 153 "ped.ragel"
{
char* field_name = strndup(ts, p-ts);
custom_field_count++;
if (custom_field_count == file->num_field) {
set_ped_record_custom_field(field_name, current_record, file);
}
}
goto st26;
st26:
if ( ++p == pe )
goto _test_eof26;
case 26:
#line 540 "ped_reader.c"
switch( (*p) ) {
case 10: goto tr46;
case 32: goto st26;
}
if ( 9 <= (*p) && (*p) <= 13 )
goto st26;
goto st0;
tr48:
#line 149 "ped.ragel"
{
ts = p;
}
goto st27;
tr52:
#line 153 "ped.ragel"
{
char* field_name = strndup(ts, p-ts);
custom_field_count++;
if (custom_field_count == file->num_field) {
set_ped_record_custom_field(field_name, current_record, file);
}
}
goto st27;
st27:
if ( ++p == pe )
goto _test_eof27;
case 27:
#line 568 "ped_reader.c"
switch( (*p) ) {
case 9: goto tr49;
case 10: goto tr50;
case 32: goto tr52;
}
if ( (*p) > 13 ) {
if ( 33 <= (*p) && (*p) <= 126 )
goto st27;
} else if ( (*p) >= 11 )
goto tr51;
goto tr44;
tr17:
#line 93 "ped.ragel"
{
ts = p;
}
goto st11;
st11:
if ( ++p == pe )
goto _test_eof11;
case 11:
#line 590 "ped_reader.c"
switch( (*p) ) {
case 9: goto tr18;
case 46: goto st12;
}
if ( 48 <= (*p) && (*p) <= 57 )
goto st11;
goto tr15;
st12:
if ( ++p == pe )
goto _test_eof12;
case 12:
if ( 48 <= (*p) && (*p) <= 57 )
goto st13;
goto tr15;
st13:
if ( ++p == pe )
goto _test_eof13;
case 13:
if ( (*p) == 9 )
goto tr18;
if ( 48 <= (*p) && (*p) <= 57 )
goto st13;
goto tr15;
tr13:
#line 81 "ped.ragel"
{
ts = p;
}
goto st14;
st14:
if ( ++p == pe )
goto _test_eof14;
case 14:
#line 624 "ped_reader.c"
switch( (*p) ) {
case 9: goto tr14;
case 95: goto st14;
}
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto st14;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto st14;
} else
goto st14;
goto tr11;
tr9:
#line 69 "ped.ragel"
{
ts = p;
}
goto st15;
st15:
if ( ++p == pe )
goto _test_eof15;
case 15:
#line 648 "ped_reader.c"
switch( (*p) ) {
case 9: goto tr10;
case 95: goto st15;
}
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto st15;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto st15;
} else
goto st15;
goto tr7;
st16:
if ( ++p == pe )
goto _test_eof16;
case 16:
switch( (*p) ) {
case 9: goto st17;
case 32: goto tr28;
case 95: goto tr29;
}
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto tr29;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto tr29;
} else
goto tr29;
goto tr26;
st17:
if ( ++p == pe )
goto _test_eof17;
case 17:
switch( (*p) ) {
case 32: goto tr29;
case 95: goto tr29;
}
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto tr29;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto tr29;
} else
goto tr29;
goto tr26;
tr29:
#line 128 "ped.ragel"
{
ts = p;
}
goto st18;
st18:
if ( ++p == pe )
goto _test_eof18;
case 18:
#line 707 "ped_reader.c"
switch( (*p) ) {
case 9: goto tr30;
case 10: goto tr31;
case 32: goto st18;
case 95: goto st18;
}
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto st18;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto st18;
} else
goto st18;
goto tr26;
tr30:
#line 132 "ped.ragel"
{
char* field_name = strndup(ts, p-ts);
custom_field_count++;
if (file->variable_field && !strcmp(field_name, file->variable_field)) {
file->num_field = custom_field_count;
}
free(field_name);
}
goto st19;
st19:
if ( ++p == pe )
goto _test_eof19;
case 19:
#line 738 "ped_reader.c"
switch( (*p) ) {
case 9: goto st19;
case 10: goto st28;
case 32: goto tr29;
case 95: goto tr29;
}
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto tr29;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto tr29;
} else
goto tr29;
goto tr26;
tr31:
#line 132 "ped.ragel"
{
char* field_name = strndup(ts, p-ts);
custom_field_count++;
if (file->variable_field && !strcmp(field_name, file->variable_field)) {
file->num_field = custom_field_count;
}
free(field_name);
}
goto st28;
st28:
if ( ++p == pe )
goto _test_eof28;
case 28:
#line 769 "ped_reader.c"
if ( (*p) == 10 )
goto st22;
if ( (*p) > 34 ) {
if ( 36 <= (*p) && (*p) <= 126 )
goto tr36;
} else if ( (*p) >= 33 )
goto tr36;
goto tr0;
tr28:
#line 128 "ped.ragel"
{
ts = p;
}
goto st20;
st20:
if ( ++p == pe )
goto _test_eof20;
case 20:
#line 788 "ped_reader.c"
switch( (*p) ) {
case 9: goto tr30;
case 10: goto tr31;
case 32: goto tr29;
case 95: goto tr29;
}
if ( (*p) < 65 ) {
if ( 48 <= (*p) && (*p) <= 57 )
goto tr29;
} else if ( (*p) > 90 ) {
if ( 97 <= (*p) && (*p) <= 122 )
goto tr29;
} else
goto tr29;
goto tr26;
}
_test_eof22: cs = 22; goto _test_eof;
_test_eof1: cs = 1; goto _test_eof;
_test_eof2: cs = 2; goto _test_eof;
_test_eof3: cs = 3; goto _test_eof;
_test_eof4: cs = 4; goto _test_eof;
_test_eof5: cs = 5; goto _test_eof;
_test_eof6: cs = 6; goto _test_eof;
_test_eof7: cs = 7; goto _test_eof;
_test_eof8: cs = 8; goto _test_eof;
_test_eof9: cs = 9; goto _test_eof;
_test_eof10: cs = 10; goto _test_eof;
_test_eof23: cs = 23; goto _test_eof;
_test_eof24: cs = 24; goto _test_eof;
_test_eof25: cs = 25; goto _test_eof;
_test_eof26: cs = 26; goto _test_eof;
_test_eof27: cs = 27; goto _test_eof;
_test_eof11: cs = 11; goto _test_eof;
_test_eof12: cs = 12; goto _test_eof;
_test_eof13: cs = 13; goto _test_eof;
_test_eof14: cs = 14; goto _test_eof;
_test_eof15: cs = 15; goto _test_eof;
_test_eof16: cs = 16; goto _test_eof;
_test_eof17: cs = 17; goto _test_eof;
_test_eof18: cs = 18; goto _test_eof;
_test_eof19: cs = 19; goto _test_eof;
_test_eof28: cs = 28; goto _test_eof;
_test_eof20: cs = 20; goto _test_eof;
_test_eof: {}
if ( p == eof )
{
switch ( cs ) {
case 24:
case 25:
case 26:
#line 27 "ped.ragel"
{
// If batch is full, add to the list of batches and create a new, empty one
if (ped_batch_is_full(current_batch))
{
list_item_t *item = list_item_new(num_records, 1, current_batch);
list_insert_item(item, batches_list);
LOG_DEBUG_F("Batch added - %zu records\n", current_batch->length);
current_batch = ped_batch_new(batch_size);
}
// Add current record to current batch
if (current_record) {
add_record_to_ped_batch(current_record, current_batch);
num_records++;
}
current_record = NULL;
}
break;
case 1:
#line 53 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'family' field\n", lines + 1, file->filename);
}
break;
case 2:
case 3:
#line 65 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'individual' field\n", lines + 1, file->filename);
}
break;
case 4:
case 5:
case 15:
#line 77 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'father' field\n", lines + 1, file->filename);
}
break;
case 6:
case 7:
case 14:
#line 89 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'mother' field\n", lines + 1, file->filename);
}
break;
case 8:
case 9:
case 11:
case 12:
case 13:
#line 109 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'sex' field\n", lines + 1, file->filename);
}
break;
case 10:
#line 124 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'phenotype' field\n", lines + 1, file->filename);
}
break;
case 16:
case 17:
case 18:
case 19:
case 20:
#line 141 "ped.ragel"
{
LOG_ERROR_F("Line %zu (%s): Error in 'header' field\n", lines + 1, file->filename);
}
break;
case 27:
#line 153 "ped.ragel"
{
char* field_name = strndup(ts, p-ts);
custom_field_count++;
if (custom_field_count == file->num_field) {
set_ped_record_custom_field(field_name, current_record, file);
}
}
#line 27 "ped.ragel"
{
// If batch is full, add to the list of batches and create a new, empty one
if (ped_batch_is_full(current_batch))
{
list_item_t *item = list_item_new(num_records, 1, current_batch);
list_insert_item(item, batches_list);
LOG_DEBUG_F("Batch added - %zu records\n", current_batch->length);
current_batch = ped_batch_new(batch_size);
}
// Add current record to current batch
if (current_record) {
add_record_to_ped_batch(current_record, current_batch);
num_records++;
}
current_record = NULL;
}
break;
case 23:
#line 117 "ped.ragel"
{
if (strncmp(".", ts, 1)) {
char *field = strndup(ts, p-ts);
set_ped_record_phenotype(field, current_record, file);
}
}
#line 145 "ped.ragel"
{
custom_field_count = 6;
}
#line 27 "ped.ragel"
{
// If batch is full, add to the list of batches and create a new, empty one
if (ped_batch_is_full(current_batch))
{
list_item_t *item = list_item_new(num_records, 1, current_batch);
list_insert_item(item, batches_list);
LOG_DEBUG_F("Batch added - %zu records\n", current_batch->length);
current_batch = ped_batch_new(batch_size);
}
// Add current record to current batch
if (current_record) {
add_record_to_ped_batch(current_record, current_batch);
num_records++;
}
current_record = NULL;
}
break;
#line 973 "ped_reader.c"
}
}
_out: {}
}
#line 221 "ped.ragel"
// Insert the last batch
if (!ped_batch_is_empty(current_batch))
{
list_item_t *item = list_item_new(num_records, 1, current_batch);
list_insert_item(item, batches_list);
LOG_DEBUG_F("Batch added - %zu records (last)\n", current_batch->length);
}
if ( cs <
#line 992 "ped_reader.c"
21
#line 231 "ped.ragel"
)
{
LOG_ERROR("The file was not successfully read\n");
LOG_INFO_F("Last state is %d, but %d was expected\n",
cs,
#line 1000 "ped_reader.c"
21
#line 235 "ped.ragel"
);
}
LOG_INFO_F("PED records read = %zu\n", num_records);
return cs <
#line 1009 "ped_reader.c"
21
#line 240 "ped.ragel"
;
}
int run_effect(char **urls, shared_options_data_t *shared_options_data, effect_options_data_t *options_data) {
int ret_code = 0;
double start, stop, total;
vcf_file_t *vcf_file = vcf_open(shared_options_data->vcf_filename, shared_options_data->max_batches);
if (!vcf_file) {
LOG_FATAL("VCF file does not exist!\n");
}
ped_file_t *ped_file = NULL;
if (shared_options_data->ped_filename) {
ped_file = ped_open(shared_options_data->ped_filename);
if (!ped_file) {
LOG_FATAL("PED file does not exist!\n");
}
LOG_INFO("About to read PED file...\n");
// Read PED file before doing any processing
ret_code = ped_read(ped_file);
if (ret_code != 0) {
LOG_FATAL_F("Can't read PED file: %s\n", ped_file->filename);
}
}
char *output_directory = shared_options_data->output_directory;
size_t output_directory_len = strlen(output_directory);
ret_code = create_directory(output_directory);
if (ret_code != 0 && errno != EEXIST) {
LOG_FATAL_F("Can't create output directory: %s\n", output_directory);
}
// Remove all .txt files in folder
ret_code = delete_files_by_extension(output_directory, "txt");
if (ret_code != 0) {
return ret_code;
}
// Initialize environment for connecting to the web service
ret_code = init_http_environment(0);
if (ret_code != 0) {
return ret_code;
}
// Output file descriptors
static cp_hashtable *output_files = NULL;
// Lines of the output data in the main .txt files
static list_t *output_list = NULL;
// Consequence type counters (for summary, must be kept between web service calls)
static cp_hashtable *summary_count = NULL;
// Gene list (for genes-with-variants, must be kept between web service calls)
static cp_hashtable *gene_list = NULL;
// Initialize collections of file descriptors and summary counters
ret_code = initialize_output_files(output_directory, output_directory_len, &output_files);
if (ret_code != 0) {
return ret_code;
}
initialize_output_data_structures(shared_options_data, &output_list, &summary_count, &gene_list);
initialize_ws_buffers(shared_options_data->num_threads);
// Create job.status file
char job_status_filename[output_directory_len + 10];
sprintf(job_status_filename, "%s/job.status", output_directory);
FILE *job_status = new_job_status_file(job_status_filename);
if (!job_status) {
LOG_FATAL("Can't create job status file\n");
} else {
update_job_status_file(0, job_status);
}
#pragma omp parallel sections private(start, stop, total)
{
#pragma omp section
{
LOG_DEBUG_F("Thread %d reads the VCF file\n", omp_get_thread_num());
start = omp_get_wtime();
ret_code = vcf_read(vcf_file, 1,
(shared_options_data->batch_bytes > 0) ? shared_options_data->batch_bytes : shared_options_data->batch_lines,
shared_options_data->batch_bytes <= 0);
stop = omp_get_wtime();
total = stop - start;
if (ret_code) {
LOG_ERROR_F("Error %d while reading the file %s\n", ret_code, vcf_file->filename);
}
LOG_INFO_F("[%dR] Time elapsed = %f s\n", omp_get_thread_num(), total);
LOG_INFO_F("[%dR] Time elapsed = %e ms\n", omp_get_thread_num(), total*1000);
notify_end_parsing(vcf_file);
}
#pragma omp section
{
// Enable nested parallelism and set the number of threads the user has chosen
omp_set_nested(1);
LOG_DEBUG_F("Thread %d processes data\n", omp_get_thread_num());
// Filters and files for filtering output
filter_t **filters = NULL;
int num_filters = 0;
if (shared_options_data->chain != NULL) {
filters = sort_filter_chain(shared_options_data->chain, &num_filters);
}
FILE *passed_file = NULL, *failed_file = NULL, *non_processed_file = NULL;
get_filtering_output_files(shared_options_data, &passed_file, &failed_file);
// Pedigree information (used in some filters)
individual_t **individuals = NULL;
khash_t(ids) *sample_ids = NULL;
// Filename structure outdir/vcfname.errors
char *prefix_filename = calloc(strlen(shared_options_data->vcf_filename), sizeof(char));
get_filename_from_path(shared_options_data->vcf_filename, prefix_filename);
char *non_processed_filename = malloc((strlen(shared_options_data->output_directory) + strlen(prefix_filename) + 9) * sizeof(char));
sprintf(non_processed_filename, "%s/%s.errors", shared_options_data->output_directory, prefix_filename);
non_processed_file = fopen(non_processed_filename, "w");
free(non_processed_filename);
// Maximum size processed by each thread (never allow more than 1000 variants per query)
if (shared_options_data->batch_lines > 0) {
shared_options_data->entries_per_thread = MIN(MAX_VARIANTS_PER_QUERY,
ceil((float) shared_options_data->batch_lines / shared_options_data->num_threads));
} else {
shared_options_data->entries_per_thread = MAX_VARIANTS_PER_QUERY;
}
LOG_DEBUG_F("entries-per-thread = %d\n", shared_options_data->entries_per_thread);
int i = 0;
vcf_batch_t *batch = NULL;
int ret_ws_0 = 0, ret_ws_1 = 0, ret_ws_2 = 0;
start = omp_get_wtime();
while (batch = fetch_vcf_batch(vcf_file)) {
if (i == 0) {
// Add headers associated to the defined filters
vcf_header_entry_t **filter_headers = get_filters_as_vcf_headers(filters, num_filters);
for (int j = 0; j < num_filters; j++) {
add_vcf_header_entry(filter_headers[j], vcf_file);
}
// Write file format, header entries and delimiter
if (passed_file != NULL) { write_vcf_header(vcf_file, passed_file); }
if (failed_file != NULL) { write_vcf_header(vcf_file, failed_file); }
if (non_processed_file != NULL) { write_vcf_header(vcf_file, non_processed_file); }
LOG_DEBUG("VCF header written\n");
if (ped_file) {
// Create map to associate the position of individuals in the list of samples defined in the VCF file
sample_ids = associate_samples_and_positions(vcf_file);
// Sort individuals in PED as defined in the VCF file
individuals = sort_individuals(vcf_file, ped_file);
}
}
// printf("batch loaded = '%.*s'\n", 50, batch->text);
// printf("batch text len = %zu\n", strlen(batch->text));
// if (i % 10 == 0) {
LOG_INFO_F("Batch %d reached by thread %d - %zu/%zu records \n",
i, omp_get_thread_num(),
batch->records->size, batch->records->capacity);
// }
int reconnections = 0;
int max_reconnections = 3; // TODO allow to configure?
// Write records that passed to a separate file, and query the WS with them as args
array_list_t *failed_records = NULL;
int num_variables = ped_file? get_num_variables(ped_file): 0;
array_list_t *passed_records = filter_records(filters, num_filters, individuals, sample_ids, num_variables, batch->records, &failed_records);
if (passed_records->size > 0) {
// Divide the list of passed records in ranges of size defined in config file
int num_chunks;
int *chunk_sizes;
int *chunk_starts = create_chunks(passed_records->size, shared_options_data->entries_per_thread, &num_chunks, &chunk_sizes);
do {
// OpenMP: Launch a thread for each range
#pragma omp parallel for num_threads(shared_options_data->num_threads)
for (int j = 0; j < num_chunks; j++) {
int tid = omp_get_thread_num();
LOG_DEBUG_F("[%d] WS invocation\n", tid);
LOG_DEBUG_F("[%d] -- effect WS\n", tid);
if (!reconnections || ret_ws_0) {
ret_ws_0 = invoke_effect_ws(urls[0], (vcf_record_t**) (passed_records->items + chunk_starts[j]),
chunk_sizes[j], options_data->excludes);
parse_effect_response(tid, output_directory, output_directory_len, output_files, output_list, summary_count, gene_list);
free(effect_line[tid]);
effect_line[tid] = (char*) calloc (max_line_size[tid], sizeof(char));
}
if (!options_data->no_phenotypes) {
if (!reconnections || ret_ws_1) {
LOG_DEBUG_F("[%d] -- snp WS\n", omp_get_thread_num());
ret_ws_1 = invoke_snp_phenotype_ws(urls[1], (vcf_record_t**) (passed_records->items + chunk_starts[j]), chunk_sizes[j]);
parse_snp_phenotype_response(tid, output_list);
free(snp_line[tid]);
snp_line[tid] = (char*) calloc (snp_max_line_size[tid], sizeof(char));
}
if (!reconnections || ret_ws_2) {
LOG_DEBUG_F("[%d] -- mutation WS\n", omp_get_thread_num());
ret_ws_2 = invoke_mutation_phenotype_ws(urls[2], (vcf_record_t**) (passed_records->items + chunk_starts[j]), chunk_sizes[j]);
parse_mutation_phenotype_response(tid, output_list);
free(mutation_line[tid]);
mutation_line[tid] = (char*) calloc (mutation_max_line_size[tid], sizeof(char));
}
}
}
LOG_DEBUG_F("*** %dth web services invocation finished\n", i);
if (ret_ws_0 || ret_ws_1 || ret_ws_2) {
if (ret_ws_0) {
LOG_ERROR_F("Effect web service error: %s\n", get_last_http_error(ret_ws_0));
}
if (ret_ws_1) {
LOG_ERROR_F("SNP phenotype web service error: %s\n", get_last_http_error(ret_ws_1));
}
if (ret_ws_2) {
LOG_ERROR_F("Mutations phenotype web service error: %s\n", get_last_http_error(ret_ws_2));
}
// In presence of errors, wait 4 seconds before retrying
reconnections++;
LOG_ERROR_F("Some errors ocurred, reconnection #%d\n", reconnections);
sleep(4);
} else {
free(chunk_starts);
free(chunk_sizes);
}
} while (reconnections < max_reconnections && (ret_ws_0 || ret_ws_1 || ret_ws_2));
}
// If the maximum number of reconnections was reached still with errors,
// write the non-processed batch to the corresponding file
if (reconnections == max_reconnections && (ret_ws_0 || ret_ws_1 || ret_ws_2)) {
#pragma omp critical
{
write_vcf_batch(batch, non_processed_file);
}
}
// Write records that passed and failed filters to separate files, and free them
write_filtering_output_files(passed_records, failed_records, passed_file, failed_file);
free_filtered_records(passed_records, failed_records, batch->records);
// Free batch and its contents
vcf_batch_free(batch);
i++;
}
stop = omp_get_wtime();
total = stop - start;
LOG_INFO_F("[%d] Time elapsed = %f s\n", omp_get_thread_num(), total);
LOG_INFO_F("[%d] Time elapsed = %e ms\n", omp_get_thread_num(), total*1000);
// Free resources
if (passed_file) { fclose(passed_file); }
if (failed_file) { fclose(failed_file); }
if (non_processed_file) { fclose(non_processed_file); }
// Free filters
for (i = 0; i < num_filters; i++) {
filter_t *filter = filters[i];
filter->free_func(filter);
}
free(filters);
// Decrease list writers count
for (i = 0; i < shared_options_data->num_threads; i++) {
list_decr_writers(output_list);
}
}
#pragma omp section
{
// Thread which writes the results to all_variants, summary and one file per consequence type
int ret = 0;
char *line;
list_item_t* item = NULL;
FILE *fd = NULL;
FILE *all_variants_file = cp_hashtable_get(output_files, "all_variants");
FILE *snp_phenotype_file = cp_hashtable_get(output_files, "snp_phenotypes");
FILE *mutation_phenotype_file = cp_hashtable_get(output_files, "mutation_phenotypes");
while ((item = list_remove_item(output_list)) != NULL) {
line = item->data_p;
// Type greater than 0: consequence type identified by its SO code
// Type equals to -1: SNP phenotype
// Type equals to -2: mutation phenotype
if (item->type > 0) {
// Write entry in the consequence type file
fd = cp_hashtable_get(output_files, &(item->type));
int ret = fprintf(fd, "%s\n", line);
if (ret < 0) {
LOG_ERROR_F("Error writing to file: '%s'\n", line);
}
// Write in all_variants
ret = fprintf(all_variants_file, "%s\n", line);
if (ret < 0) {
LOG_ERROR_F("Error writing to all_variants: '%s'\n", line);
}
} else if (item->type == SNP_PHENOTYPE) {
ret = fprintf(snp_phenotype_file, "%s\n", line);
if (ret < 0) {
LOG_ERROR_F("Error writing to snp_phenotypes: '%s'\n", line);
}
} else if (item->type == MUTATION_PHENOTYPE) {
ret = fprintf(mutation_phenotype_file, "%s\n", line);
if (ret < 0) {
LOG_ERROR_F("Error writing to mutation_phenotypes: '%s'\n", line);
}
}
free(line);
list_item_free(item);
}
}
}
write_summary_file(summary_count, cp_hashtable_get(output_files, "summary"));
write_genes_with_variants_file(gene_list, output_directory);
write_result_file(shared_options_data, options_data, summary_count, output_directory);
free_output_data_structures(output_files, summary_count, gene_list);
free_ws_buffers(shared_options_data->num_threads);
free(output_list);
vcf_close(vcf_file);
update_job_status_file(100, job_status);
close_job_status_file(job_status);
return ret_code;
}
int main (int argc, char *argv[])
{
size_t max_batches = 20;
size_t batch_size = 2000;
list_t *read_list = (list_t*) malloc (sizeof(list_t));
list_init("batches", 1, max_batches, read_list);
int ret_code;
double start, stop, total;
char *filename = (char*) malloc ((strlen(argv[1])+1) * sizeof(char));
strncat(filename, argv[1], strlen(argv[1]));
gff_file_t* file;
init_log_custom(LOG_LEVEL_DEBUG, 1, NULL, "w");
#pragma omp parallel sections private(start, stop, total) lastprivate(file)
{
#pragma omp section
{
LOG_DEBUG_F("Thread %d reads the GFF file\n", omp_get_thread_num());
// Reading
start = omp_get_wtime();
file = gff_open(filename);
ret_code = gff_read_batches(read_list, batch_size, file);
stop = omp_get_wtime();
total = (stop - start);
if (ret_code) {
LOG_FATAL_F("[%dR] Error code = %d\n", omp_get_thread_num(), ret_code);
}
LOG_INFO_F("[%dR] Time elapsed = %f s\n", omp_get_thread_num(), total);
LOG_INFO_F("[%dR] Time elapsed = %e ms\n", omp_get_thread_num(), total*1000);
// Writing to a new file
if (argc == 3)
{
start = omp_get_wtime();
ret_code = gff_write(file, argv[2]);
stop = omp_get_wtime();
total = (stop - start);
if (ret_code) {
LOG_ERROR_F("[%dW] Error code = %d\n", omp_get_thread_num(), ret_code);
}
LOG_INFO_F("[%dW] Time elapsed = %f s\n", omp_get_thread_num(), total);
LOG_INFO_F("[%dW] Time elapsed = %e ms\n", omp_get_thread_num(), total*1000);
}
list_decr_writers(read_list);
gff_close(file, 0);
}
#pragma omp section
{
printf("1st log debug\n");
LOG_DEBUG_F("OMP num threads = %d\n", omp_get_num_threads());
LOG_DEBUG_F("Thread %d prints info\n", omp_get_thread_num());
printf("after 1st log debug\n");
start = omp_get_wtime();
int i = 0;
list_item_t* item = NULL;
FILE *out = fopen("result.gff", "w");
while ( (item = list_remove_item(read_list)) != NULL ) {
if (i % 200 == 0)
{
int debug = 1;
LOG_DEBUG_F("Batch %d reached by thread %d - %zu/%zu records \n", i, omp_get_thread_num(),
((gff_batch_t*) item->data_p)->length, ((gff_batch_t*) item->data_p)->max_length);
}
// gff_write_to_file(file, out);
// gff_batch_print(stdout, item->data_p);
write_gff_batch(item->data_p, out);
gff_batch_free(item->data_p);
list_item_free(item);
i++;
}
fclose(out);
stop = omp_get_wtime();
total = (stop - start);
LOG_INFO_F("[%d] Time elapsed = %f s\n", omp_get_thread_num(), total);
LOG_INFO_F("[%d] Time elapsed = %e ms\n", omp_get_thread_num(), total*1000);
}
}
free(read_list);
return 0;
}
/**
* Initialize and activate timing of a context.
*/
int
bfwork_context_init_timing(bfwork_context_t *context, const char *tag, const char *path_folder)
{
int err;
char *sched;
char filename[100];
char intaux[20];
assert(context);
//Set tag
context->tag = (char *) tag;
//Create timing
if(time_new_stats(20, &context->time_stats))
{
LOG_ERROR("Failed to initialize time stats\n");
}
//Get OMP schedule
sched = getenv("OMP_SCHEDULE");
//Target folder for stats?
if(path_folder != NULL)
{
strcpy(filename, path_folder);
//Create stats directory
err = mkdir(filename, S_IRWXU);
if(err)
{
err = errno;
LOG_WARN_F("Failed to create stats directory \"%s\", error code: %s\n", filename, strerror(err));
}
strcat(filename, "/");
if(tag)
{
strcat(filename,tag);
}
if(sched)
{
strcat(filename,"_");
strcat(filename,sched);
}
else
{
printf("ERROR: Obtaining OMP_SCHEDULE environment value\n");
}
//strcat(filename,"_");
//sprintf(intaux, "%d", MAX_BATCH_SIZE);
//strcat(filename, intaux);
strcat(filename, "_");
sprintf(intaux, "%d", omp_get_max_threads());
strcat(filename, intaux);
strcat(filename, ".stats");
//Set output file
if(time_set_output_file(filename, context->time_stats))
{
LOG_ERROR_F("Failed to set timing file output to \"%s\"\n", filename);
}
else
{
printf("STATISTICS ACTIVATED, output file: %s\n\n", filename);
}
}
return NO_ERROR;
}
/**
* PRIVATE. Wander for a region.
*/
static inline int
bfwork_obtain_region(bam_fwork_t *fwork, bam_region_t *region)
{
int err, bytes;
bam1_t *read;
double times;
//Get first read
if(last_read != NULL)
{
read = last_read;
bytes = last_read_bytes;
last_read = NULL;
}
else
{
//Get first read from file
read = bam_init1();
assert(read);
bytes = bam_read1(fwork->input_file->bam_fd, read);
}
//Iterate reads
while(bytes > 0)
{
//Wander this read
omp_set_lock(®ion->lock);
#ifdef D_TIME_DEBUG
times = omp_get_wtime();
#endif
err = fwork->context->wander_f(fwork, region, read);
#ifdef D_TIME_DEBUG
times = omp_get_wtime() - times;
if(fwork->context->time_stats)
time_add_time_slot(D_FWORK_WANDER_FUNC, fwork->context->time_stats, times);
#endif
omp_unset_lock(®ion->lock);
switch(err)
{
case WANDER_READ_FILTERED:
//This read dont pass the filters
case NO_ERROR:
//Add read to region
omp_set_lock(®ion->lock);
region->reads[region->size] = read;
region->size++;
//Region is full?
if(region->size >= region->max_size)
{
omp_unset_lock(®ion->lock);
return WANDER_REGION_CHANGED;
}
omp_unset_lock(®ion->lock);
//Get next read from file
read = bam_init1();
assert(read);
bytes = bam_read1(fwork->input_file->bam_fd, read);
break;
case WANDER_REGION_CHANGED:
//The region have changed
last_read = read;
last_read_bytes = bytes;
return err;
default:
//Unknown error
LOG_ERROR_F("Framework fails with error code: %d\n", err);
return err;
}
}
//Check read error
if(bytes <= 0)
{
//Destroy bam
bam_destroy1(read);
//End of file
if(bytes == -1)
{
return WANDER_READ_EOF;
}
else
{
return WANDER_READ_TRUNCATED;
}
}
return NO_ERROR;
}
int run_stats(shared_options_data_t *shared_options_data, stats_options_data_t *options_data) {
file_stats_t *file_stats = file_stats_new();
sample_stats_t **sample_stats;
// List that stores the batches of records filtered by each thread
list_t *output_list[shared_options_data->num_threads];
// List that stores which thread filtered the next batch to save
list_t *next_token_list = malloc(sizeof(list_t));
int ret_code;
double start, stop, total;
vcf_file_t *vcf_file = vcf_open(shared_options_data->vcf_filename, shared_options_data->max_batches);
if (!vcf_file) {
LOG_FATAL("VCF file does not exist!\n");
}
ped_file_t *ped_file = NULL;
if (shared_options_data->ped_filename) {
ped_file = ped_open(shared_options_data->ped_filename);
if (!ped_file) {
LOG_FATAL("PED file does not exist!\n");
}
if(options_data->variable) {
set_variable_field(options_data->variable, 0, ped_file);
} else {
set_variable_field("PHENO", 6, ped_file);
}
if(options_data->variable_groups) {
int n, m;
char *variable_groups = strdup(options_data->variable_groups);
char **groups;
char **phenos_in_group;
groups = split(variable_groups, ":", &n);
for(int i = 0; i < n; i++){
phenos_in_group = split(groups[i], ",", &m);
if(set_phenotype_group(phenos_in_group, m, ped_file) < 0) {
LOG_ERROR("Variable can't appear in two groups\n");
return DUPLICATED_VARIABLE;
}
free(phenos_in_group);
}
ped_file->accept_new_values = 0;
free(variable_groups);
free(groups);
} else {
ped_file->accept_new_values = 1;
}
if(options_data->phenotype) {
int n;
char* phenotypes = strdup(options_data->phenotype);
char** pheno_values = split(phenotypes, ",", &n);
if(n != 2) {
LOG_ERROR("To handle case-control test, only two phenotypes are supported\n");
return MORE_THAN_TWO_PHENOTYPES;
} else {
set_unaffected_phenotype(pheno_values[0],ped_file);
set_affected_phenotype(pheno_values[1],ped_file);
}
} else {
set_unaffected_phenotype("1", ped_file);
set_affected_phenotype("2", ped_file);
}
LOG_INFO("About to read PED file...\n");
// Read PED file before doing any processing
ret_code = ped_read(ped_file);
if (ret_code != 0) {
LOG_FATAL_F("Can't read PED file: %s\n", ped_file->filename);
}
if(!ped_file->num_field) {
LOG_ERROR_F("Can't find the specified field \"%s\" in file: %s \n", options_data->variable, ped_file->filename);
return VARIABLE_FIELD_NOT_FOUND;
}
}
ret_code = create_directory(shared_options_data->output_directory);
if (ret_code != 0 && errno != EEXIST) {
LOG_FATAL_F("Can't create output directory: %s\n", shared_options_data->output_directory);
}
// Initialize variables related to the different threads
for (int i = 0; i < shared_options_data->num_threads; i++) {
output_list[i] = (list_t*) malloc(sizeof(list_t));
list_init("input", 1, shared_options_data->num_threads * shared_options_data->batch_lines, output_list[i]);
}
list_init("next_token", shared_options_data->num_threads, INT_MAX, next_token_list);
LOG_INFO("About to retrieve statistics from VCF file...\n");
#pragma omp parallel sections private(start, stop, total)
{
#pragma omp section
{
LOG_DEBUG_F("Thread %d reads the VCF file\n", omp_get_thread_num());
// Reading
start = omp_get_wtime();
if (shared_options_data->batch_bytes > 0) {
ret_code = vcf_parse_batches_in_bytes(shared_options_data->batch_bytes, vcf_file);
} else if (shared_options_data->batch_lines > 0) {
ret_code = vcf_parse_batches(shared_options_data->batch_lines, vcf_file);
}
stop = omp_get_wtime();
total = stop - start;
if (ret_code) { LOG_FATAL_F("[%dR] Error code = %d\n", omp_get_thread_num(), ret_code); }
LOG_INFO_F("[%dR] Time elapsed = %f s\n", omp_get_thread_num(), total);
LOG_INFO_F("[%dR] Time elapsed = %e ms\n", omp_get_thread_num(), total*1000);
notify_end_parsing(vcf_file);
}
#pragma omp section
{
// Enable nested parallelism and set the number of threads the user has chosen
omp_set_nested(1);
LOG_DEBUG_F("Thread %d processes data\n", omp_get_thread_num());
individual_t **individuals = NULL;
khash_t(ids) *sample_ids = NULL;
khash_t(str) *phenotype_ids = NULL;
int num_phenotypes;
start = omp_get_wtime();
int i = 0;
vcf_batch_t *batch = NULL;
while ((batch = fetch_vcf_batch(vcf_file)) != NULL) {
if (i == 0) {
sample_stats = malloc (get_num_vcf_samples(vcf_file) * sizeof(sample_stats_t*));
for (int j = 0; j < get_num_vcf_samples(vcf_file); j++) {
sample_stats[j] = sample_stats_new(array_list_get(j, vcf_file->samples_names));
}
if (ped_file) {
// Create map to associate the position of individuals in the list of samples defined in the VCF file
sample_ids = associate_samples_and_positions(vcf_file);
// Sort individuals in PED as defined in the VCF file
individuals = sort_individuals(vcf_file, ped_file);
// Get the khash of the phenotypes in PED file
phenotype_ids = get_phenotypes(ped_file);
num_phenotypes = get_num_variables(ped_file);
}
}
if (i % 50 == 0) {
LOG_INFO_F("Batch %d reached by thread %d - %zu/%zu records \n",
i, omp_get_thread_num(),
batch->records->size, batch->records->capacity);
}
// Divide the list of passed records in ranges of size defined in config file
int num_chunks;
int *chunk_sizes = NULL;
array_list_t *input_records = batch->records;
int *chunk_starts = create_chunks(input_records->size,
ceil((float) shared_options_data->batch_lines / shared_options_data->num_threads),
&num_chunks, &chunk_sizes);
// OpenMP: Launch a thread for each range
#pragma omp parallel for num_threads(shared_options_data->num_threads)
for (int j = 0; j < num_chunks; j++) {
LOG_DEBUG_F("[%d] Stats invocation\n", omp_get_thread_num());
// Invoke variant stats and/or sample stats when applies
if (options_data->variant_stats) {
int index = omp_get_thread_num() % shared_options_data->num_threads;
ret_code = get_variants_stats((vcf_record_t**) (input_records->items + chunk_starts[j]),
chunk_sizes[j], individuals, sample_ids,num_phenotypes, output_list[index], file_stats);
}
if (options_data->sample_stats) {
ret_code |= get_sample_stats((vcf_record_t**) (input_records->items + chunk_starts[j]),
chunk_sizes[j], individuals, sample_ids, sample_stats, file_stats);
}
}
if (options_data->variant_stats) {
// Insert as many tokens as elements correspond to each thread
for (int t = 0; t < num_chunks; t++) {
for (int s = 0; s < chunk_sizes[t]; s++) {
list_item_t *token_item = list_item_new(t, 0, NULL);
list_insert_item(token_item, next_token_list);
}
}
}
free(chunk_starts);
free(chunk_sizes);
vcf_batch_free(batch);
i++;
}
stop = omp_get_wtime();
total = stop - start;
LOG_INFO_F("[%d] Time elapsed = %f s\n", omp_get_thread_num(), total);
LOG_INFO_F("[%d] Time elapsed = %e ms\n", omp_get_thread_num(), total*1000);
// Decrease list writers count
for (i = 0; i < shared_options_data->num_threads; i++) {
list_decr_writers(next_token_list);
list_decr_writers(output_list[i]);
}
if (sample_ids) { kh_destroy(ids, sample_ids); }
if (individuals) { free(individuals); }
}
#pragma omp section
{
LOG_DEBUG_F("Thread %d writes the output\n", omp_get_thread_num());
char *stats_prefix = get_vcf_stats_filename_prefix(shared_options_data->vcf_filename,
shared_options_data->output_filename,
shared_options_data->output_directory);
// File names and descriptors for output to plain text files
char *stats_filename, *summary_filename, *phenotype_filename;
FILE *stats_fd, *summary_fd, **phenotype_fd;
char *stats_db_name;
sqlite3 *db = NULL;
khash_t(stats_chunks) *hash;
khash_t(str) *phenotype_ids;
int num_phenotypes;
if(ped_file){
phenotype_ids = get_phenotypes(ped_file);
num_phenotypes = get_num_variables(ped_file);
}
if (options_data->save_db) {
delete_files_by_extension(shared_options_data->output_directory, "db");
stats_db_name = calloc(strlen(stats_prefix) + strlen(".db") + 2, sizeof(char));
sprintf(stats_db_name, "%s.db", stats_prefix);
create_stats_db(stats_db_name, VCF_CHUNKSIZE, create_vcf_query_fields, &db);
hash = kh_init(stats_chunks);
}
// Write variant (and global) statistics
if (options_data->variant_stats) {
stats_filename = get_variant_stats_output_filename(stats_prefix);
if (!(stats_fd = fopen(stats_filename, "w"))) {
LOG_FATAL_F("Can't open file for writing statistics of variants: %s\n", stats_filename);
}
//Open one file for each phenotype
if(ped_file){
phenotype_fd = malloc(sizeof(FILE*)*num_phenotypes);
if(options_data->variable_groups){
int n;
char *variable_groups = strdup(options_data->variable_groups);
char ** names = split(variable_groups, ":", &n);
for(int i = 0; i < n; i++) {
phenotype_filename = get_variant_phenotype_stats_output_filename(stats_prefix, names[i]);
if(!(phenotype_fd[i] = fopen(phenotype_filename, "w"))) {
LOG_FATAL_F("Can't open file for writing statistics of variants per phenotype: %s\n", stats_filename);
}
free(phenotype_filename);
}
free(names);
free(variable_groups);
} else {
for (khint_t i = kh_begin(phenotype_ids); i != kh_end(phenotype_ids); ++i) {
if (!kh_exist(phenotype_ids,i)) continue;
phenotype_filename = get_variant_phenotype_stats_output_filename(stats_prefix, kh_key(phenotype_ids,i));
if(!(phenotype_fd[kh_val(phenotype_ids,i)] = fopen(phenotype_filename, "w"))) {
LOG_FATAL_F("Can't open file for writing statistics of variants per phenotype: %s\n", stats_filename);
}
free(phenotype_filename);
}
}
}
// Write header
report_vcf_variant_stats_header(stats_fd);
if(ped_file){
for(int i = 0; i < num_phenotypes; i++)
report_vcf_variant_phenotype_stats_header(phenotype_fd[i]);
}
// For each variant, generate a new line
int avail_stats = 0;
variant_stats_t *var_stats_batch[VCF_CHUNKSIZE];
list_item_t *token_item = NULL, *output_item = NULL;
while ( token_item = list_remove_item(next_token_list) ) {
output_item = list_remove_item(output_list[token_item->id]);
assert(output_item);
var_stats_batch[avail_stats] = output_item->data_p;
avail_stats++;
// Run only when certain amount of stats is available
if (avail_stats >= VCF_CHUNKSIZE) {
report_vcf_variant_stats(stats_fd, db, hash, avail_stats, var_stats_batch);
if(ped_file)
for(int i = 0; i < num_phenotypes; i++)
report_vcf_variant_phenotype_stats(phenotype_fd[i], avail_stats, var_stats_batch, i);
// Free all stats from the "batch"
for (int i = 0; i < avail_stats; i++) {
variant_stats_free(var_stats_batch[i]);
}
avail_stats = 0;
}
// Free resources
list_item_free(output_item);
list_item_free(token_item);
}
if (avail_stats > 0) {
report_vcf_variant_stats(stats_fd, db, hash, avail_stats, var_stats_batch);
if(ped_file)
for(int i = 0; i < num_phenotypes; i++)
report_vcf_variant_phenotype_stats(phenotype_fd[i], avail_stats, var_stats_batch, i);
// Free all stats from the "batch"
for (int i = 0; i < avail_stats; i++) {
variant_stats_free(var_stats_batch[i]);
}
avail_stats = 0;
}
// Write whole file stats (data only got when launching variant stats)
summary_filename = get_vcf_file_stats_output_filename(stats_prefix);
if (!(summary_fd = fopen(summary_filename, "w"))) {
LOG_FATAL_F("Can't open file for writing statistics summary: %s\n", summary_filename);
}
report_vcf_summary_stats(summary_fd, db, file_stats);
free(stats_filename);
free(summary_filename);
// Close variant stats file
if (stats_fd) { fclose(stats_fd); }
if (summary_fd) { fclose(summary_fd); }
if(ped_file){
for(int i = 0; i < num_phenotypes; i++)
if(phenotype_fd[i]) fclose(phenotype_fd[i]);
free(phenotype_fd);
}
}
// Write sample statistics
if (options_data->sample_stats) {
stats_filename = get_sample_stats_output_filename(stats_prefix);
if (!(stats_fd = fopen(stats_filename, "w"))) {
LOG_FATAL_F("Can't open file for writing statistics of samples: %s\n", stats_filename);
}
report_vcf_sample_stats_header(stats_fd);
report_vcf_sample_stats(stats_fd, NULL, vcf_file->samples_names->size, sample_stats);
// Close sample stats file
free(stats_filename);
if (stats_fd) { fclose(stats_fd); }
}
free(stats_prefix);
if (db) {
insert_chunk_hash(VCF_CHUNKSIZE, hash, db);
create_stats_index(create_vcf_index, db);
close_stats_db(db, hash);
}
}
}
for (int i = 0; i < get_num_vcf_samples(vcf_file); i++) {
sample_stats_free(sample_stats[i]);
}
free(sample_stats);
free(file_stats);
free(next_token_list);
for (int i = 0; i < shared_options_data->num_threads; i++) {
free(output_list[i]);
}
vcf_close(vcf_file);
if (ped_file) { ped_close(ped_file, 1,1); }
return 0;
}
int run_merge(shared_options_data_t *shared_options_data, merge_options_data_t *options_data) {
if (options_data->num_files == 1) {
LOG_INFO("Just one VCF file specified, no need to merge");
return 0;
}
list_t *read_list[options_data->num_files];
memset(read_list, 0, options_data->num_files * sizeof(list_t*));
list_t *output_header_list = (list_t*) malloc (sizeof(list_t));
list_init("headers", shared_options_data->num_threads, INT_MAX, output_header_list);
list_t *output_list = (list_t*) malloc (sizeof(list_t));
list_init("output", shared_options_data->num_threads, shared_options_data->max_batches * shared_options_data->batch_lines, output_list);
list_t *merge_tokens = (list_t*) malloc (sizeof(list_t));
list_init("tokens", 1, INT_MAX, merge_tokens);
int ret_code = 0;
double start, stop, total;
vcf_file_t *files[options_data->num_files];
memset(files, 0, options_data->num_files * sizeof(vcf_file_t*));
// Initialize variables related to the different files
for (int i = 0; i < options_data->num_files; i++) {
files[i] = vcf_open(options_data->input_files[i], shared_options_data->max_batches);
if (!files[i]) {
LOG_FATAL_F("VCF file %s does not exist!\n", options_data->input_files[i]);
}
read_list[i] = (list_t*) malloc(sizeof(list_t));
list_init("text", 1, shared_options_data->max_batches, read_list[i]);
}
ret_code = create_directory(shared_options_data->output_directory);
if (ret_code != 0 && errno != EEXIST) {
LOG_FATAL_F("Can't create output directory: %s\n", shared_options_data->output_directory);
}
chromosome_order = get_chromosome_order(shared_options_data->host_url, shared_options_data->species,
shared_options_data->version, &num_chromosomes);
printf("Number of threads = %d\n", shared_options_data->num_threads);
#pragma omp parallel sections private(start, stop, total)
{
#pragma omp section
{
LOG_DEBUG_F("Thread %d reads the VCF file\n", omp_get_thread_num());
// Reading
start = omp_get_wtime();
ret_code = vcf_multiread_batches(read_list, shared_options_data->batch_lines, files, options_data->num_files);
stop = omp_get_wtime();
total = stop - start;
if (ret_code) {
LOG_ERROR_F("Error %d while reading VCF files\n", ret_code);
}
LOG_INFO_F("[%dR] Time elapsed = %f s\n", omp_get_thread_num(), total);
LOG_INFO_F("[%dR] Time elapsed = %e ms\n", omp_get_thread_num(), total*1000);
}
#pragma omp section
{
// Enable nested parallelism
omp_set_nested(1);
LOG_DEBUG_F("Thread %d processes data\n", omp_get_thread_num());
int num_eof_found = 0;
int eof_found[options_data->num_files];
memset(eof_found, 0, options_data->num_files * sizeof(int));
list_item_t *items[options_data->num_files];
memset(items, 0, options_data->num_files * sizeof(list_item_t*));
char *texts[options_data->num_files];
memset(texts, 0, options_data->num_files * sizeof(char*));
khash_t(pos) *positions_read = kh_init(pos);
long max_position_merged = LONG_MAX;
char *max_chromosome_merged = NULL;
int header_merged = 0;
int token = 0;
double start_parsing, start_insertion, total_parsing = 0, total_insertion = 0;
start = omp_get_wtime();
while (num_eof_found < options_data->num_files) {
/* Process:
* - N threads getting batches of VCF records and inserting them in a data structure. The common minimum
* position of each group of batches will also be stored.
* - If the data structure reaches certain size or the end of a chromosome, merge positions prior to the
* last minimum registered.
*/
// Getting text elements in a critical region guarantees that each thread gets variants in positions in the same range
for (int i = 0; i < options_data->num_files; i++) {
if (eof_found[i]) {
continue;
}
items[i] = list_remove_item(read_list[i]);
if (items[i] == NULL || !strcmp(items[i]->data_p, "")) {
LOG_INFO_F("[%d] EOF found in file %s\n", omp_get_thread_num(), options_data->input_files[i]);
eof_found[i] = 1;
num_eof_found++;
if(items[i] != NULL && !strcmp(items[i]->data_p, "")) {
free(items[i]->data_p);
list_item_free(items[i]);
LOG_DEBUG_F("[%d] Text batch freed\n", omp_get_thread_num());
} else {
LOG_DEBUG_F("[%d] No need to free text batch\n", omp_get_thread_num());
}
continue;
}
assert(items[i]->data_p != NULL);
texts[i] = items[i]->data_p;
// printf("[%d] text batch from file %d\tcontents = '%s'\n", omp_get_thread_num(), i, texts[i]);
}
for (int i = 0; i < options_data->num_files; i++) {
if (eof_found[i]) {
continue;
}
start_parsing = omp_get_wtime();
char *text_begin = texts[i];
char *text_end = text_begin + strlen(text_begin);
assert(text_end != NULL);
// printf("batch = '%.*s'\n", text_end - text_begin, text_begin);
// Get VCF batches from text batches
vcf_reader_status *status = vcf_reader_status_new(shared_options_data->batch_lines, 0);
ret_code = run_vcf_parser(text_begin, text_end, shared_options_data->batch_lines, files[i], status);
if (ret_code) {
// TODO stop?
LOG_ERROR_F("Error %d while reading the file %s\n", ret_code, files[i]->filename);
continue;
}
// printf("batches = %d\n", files[i]->record_batches->length);
vcf_batch_t *batch = fetch_vcf_batch_non_blocking(files[i]);
if (!batch) {
continue;
}
total_parsing += omp_get_wtime() - start_parsing;
start_insertion = omp_get_wtime();
// Insert records into hashtable
for (int j = 0; j < batch->records->size; j++) {
vcf_record_t *record = vcf_record_copy(array_list_get(j, batch->records));
vcf_record_file_link *link = vcf_record_file_link_new(record, files[i]);
char key[64];
compose_key_value(record->chromosome, record->position, key);
int ret = insert_position_read(key, link, positions_read);
assert(ret);
}
total_insertion += omp_get_wtime() - start_insertion;
// Update minimum position being a maximum of these batches
vcf_record_t *current_record = (vcf_record_t*) array_list_get(batch->records->size - 1, batch->records);
calculate_merge_interval(current_record, &max_chromosome_merged, &max_position_merged, chromosome_order, num_chromosomes);
// Free batch and its contents
vcf_reader_status_free(status);
vcf_batch_free(batch);
list_item_free(items[i]);
}
if (num_eof_found == options_data->num_files) {
max_chromosome_merged = chromosome_order[num_chromosomes-1];
max_position_merged = LONG_MAX;
}
// Merge headers, if not previously done
if (!header_merged) {
merge_vcf_headers(files, options_data->num_files, options_data, output_header_list);
header_merged = 1;
// Decrease list writers count
for (int i = 0; i < shared_options_data->num_threads; i++) {
list_decr_writers(output_header_list);
}
}
// If the data structure reaches certain size or the end of a chromosome,
// merge positions prior to the last minimum registered
if (num_eof_found < options_data->num_files && kh_size(positions_read) > TREE_LIMIT) {
LOG_INFO_F("Merging until position %s:%ld\n", max_chromosome_merged, max_position_merged);
token = merge_interval(positions_read, max_chromosome_merged, max_position_merged, chromosome_order, num_chromosomes,
files, shared_options_data, options_data, output_list);
}
// When reaching EOF for all files, merge the remaining entries
else if (num_eof_found == options_data->num_files && kh_size(positions_read) > 0) {
LOG_INFO_F("Merging remaining positions (last = %s:%ld)\n", chromosome_order[num_chromosomes - 1], LONG_MAX);
token = merge_remaining_interval(positions_read, files, shared_options_data, options_data, output_list);
}
if (token) {
int *token_ptr = malloc (sizeof(int)); *token_ptr = token;
list_item_t *item = list_item_new(1, 0, token_ptr);
list_insert_item(item, merge_tokens);
}
// Set variables ready for next iteration of the algorithm
if (max_chromosome_merged) {
free(max_chromosome_merged);
}
token = 0;
max_chromosome_merged = NULL;
max_position_merged = LONG_MAX;
}
kh_destroy(pos, positions_read);
stop = omp_get_wtime();
total = stop - start;
LOG_INFO_F("[%d] Time elapsed = %f s\n", omp_get_thread_num(), total);
LOG_INFO_F("[%d] Time elapsed = %e ms\n", omp_get_thread_num(), total*1000);
LOG_DEBUG_F("** Time in parsing = %f s\n", total_parsing);
LOG_DEBUG_F("** Time in insertion = %f s\n", total_insertion);
// for (int i = 0; i < shared_options_data->num_threads; i++) {
// printf("[%d] Time in searching = %f s\n", i, total_search[i]);
// printf("[%d] Time in merging = %f s\n", i, total_merge[i]);
// }
// Decrease list writers count
for (int i = 0; i < shared_options_data->num_threads; i++) {
list_decr_writers(output_list);
}
list_decr_writers(merge_tokens);
}
#pragma omp section
{
LOG_DEBUG_F("Thread %d writes the output\n", omp_get_thread_num());
start = omp_get_wtime();
// Create file streams for results
char aux_filename[32]; memset(aux_filename, 0, 32 * sizeof(char));
sprintf(aux_filename, "merge_from_%d_files.vcf", options_data->num_files);
char *merge_filename;
FILE *merge_fd = get_output_file(shared_options_data, aux_filename, &merge_filename);
LOG_INFO_F("Output filename = %s\n", merge_filename);
free(merge_filename);
list_item_t *item1 = NULL, *item2 = NULL;
vcf_header_entry_t *entry;
vcf_record_t *record;
int *num_records;
// Write headers
while ((item1 = list_remove_item(output_header_list)) != NULL) {
entry = item1->data_p;
write_vcf_header_entry(entry, merge_fd);
}
// Write delimiter
array_list_t *sample_names = merge_vcf_sample_names(files, options_data->num_files);
write_vcf_delimiter_from_samples((char**) sample_names->items, sample_names->size, merge_fd);
// Write records
// When a token is present, it means a set of batches has been merged. The token contains the number of records merged.
// In this case, the records must be sorted by chromosome and position, and written afterwards.
while ((item1 = list_remove_item(merge_tokens)) != NULL) {
num_records = item1->data_p;
vcf_record_t *records[*num_records];
for (int i = 0; i < *num_records; i++) {
item2 = list_remove_item(output_list);
if (!item2) {
break;
}
records[i] = item2->data_p;
list_item_free(item2);
}
// Sort records
qsort(records, *num_records, sizeof(vcf_record_t*), record_cmp);
// Write and free sorted records
for (int i = 0; i < *num_records; i++) {
record = records[i];
write_vcf_record(record, merge_fd);
vcf_record_free_deep(record);
}
free(num_records);
list_item_free(item1);
}
// Close file
if (merge_fd != NULL) { fclose(merge_fd); }
stop = omp_get_wtime();
total = stop - start;
LOG_INFO_F("[%dW] Time elapsed = %f s\n", omp_get_thread_num(), total);
LOG_INFO_F("[%dW] Time elapsed = %e ms\n", omp_get_thread_num(), total*1000);
}
}
// Free variables related to the different files
for (int i = 0; i < options_data->num_files; i++) {
if(files[i]) { vcf_close(files[i]); }
if(read_list[i]) { free(read_list[i]); }
}
free(output_list);
return ret_code;
}