static AjPTable btwisted_getdinucdata(AjPFile inf)
{
AjPStr valstr = NULL;
AjPStr key = NULL;
AjPStr line = NULL;
AjPStrTok token = NULL;
AjPTable table = NULL;
valstr = ajStrNew();
line = ajStrNew();
table = ajTablestrNewCase(20);
while(ajReadlineTrim(inf,&line))
{
if(*ajStrGetPtr(line)=='#')
continue;
token = ajStrTokenNewC(line," \n\t\r");
key = ajStrNew();
ajStrTokenNextParseC(&token," \n\t\r",&key);
valstr = ajStrNew();
ajStrTokenNextParseC(&token," \n\t\r",&valstr);
ajTablePut(table,(void *)key,(void *) valstr);
ajStrTokenDel(&token);
}
ajStrDel(&line);
return table;
}
static ajint cutgextract_readcodons(AjPFile inf, AjBool allrecords,
ajint *count)
{
static int cutidx[] =
{
42,43,46,41,45,44,26,30,31,29,27,28,48,51,47,50,
52,49,55,56,53,54,36,38,35,37, 4, 6, 3, 5,17,18,
16,15,57,59,60,58,24,25,34,33,39,40,20,19,11,12,
10, 9,63,62, 8, 7,14,13,21,23,22,32,61, 1, 0, 2
};
AjPStr line = NULL;
AjPStr value = NULL;
ajint thiscount[64];
AjPStrTok token = NULL;
ajint i;
ajint n = 0;
ajint nstops = 0;
if(!line)
{
line = ajStrNew();
value = ajStrNew();
}
if(!ajReadlineTrim(inf,&line))
ajFatal("Premature end of file");
token = ajStrTokenNewC(line," \n\t\r");
for(i=0;i<CODONS;++i)
{
ajStrTokenNextParseC(&token," \n\t\r",&value);
ajStrToInt(value,&n);
thiscount[cutidx[i]] = n;
if(i>60)
nstops += n;
}
ajStrDel(&line);
ajStrDel(&value);
ajStrTokenDel(&token);
if(!allrecords)
if(nstops > 1)
return -1;
for(i=0;i<CODONS;++i)
{
count[i] += thiscount[i];
}
return nstops;
}
static void remap_NoCutList(AjPFile outfile, const AjPTable hittable,
AjBool html, const AjPStr enzymes, AjBool blunt,
AjBool sticky, ajuint sitelen, AjBool commercial,
AjBool ambiguity, AjBool limit,
const AjPTable retable)
{
/* for iterating over hittable */
PValue value;
void **keyarray = NULL; /* array for table */
void **valarray = NULL; /* array for table */
ajint i;
/* list of enzymes that cut */
AjPList cutlist;
AjIList citer; /* iterator for cutlist */
AjPStr cutname = NULL;
AjBool found;
/* for parsing value->iso string */
AjPStrTok tok;
char tokens[] = " ,";
AjPStr code = NULL;
const char *p;
/* for reading in enzymes names */
AjPFile enzfile = NULL;
AjPStr *ea;
ajint ne; /* number of enzymes */
AjBool isall = ajTrue;
/* list of enzymes that don't cut */
AjPList nocutlist;
AjIList niter; /* iterator for nocutlist */
AjPStr nocutname = NULL;
/* count of rejected enzymes not matching criteria */
ajint rejected_count = 0;
EmbPPatRestrict enz;
/* for renaming preferred isoschizomers */
AjPList newlist;
/*
**
** Make a list of enzymes('cutlist') that hit
** including the isoschizomer names
**
*/
ajDebug("Make a list of all enzymes that cut\n");
cutlist = ajListstrNew();
nocutlist = ajListstrNew();
ajTableToarrayKeysValues(hittable, &keyarray, &valarray);
for(i = 0; keyarray[i]; i++)
{
value = (PValue) valarray[i];
cutname = ajStrNew();
ajStrAssignRef(&cutname, keyarray[i]);
ajListstrPushAppend(cutlist, cutname);
/* Add to cutlist all isoschizomers of enzymes that cut */
ajDebug("Add to cutlist all isoschizomers of enzymes that cut\n");
/* start token to parse isoschizomers names */
tok = ajStrTokenNewC(value->iso, tokens);
while(ajStrTokenNextParseC(&tok, tokens, &code))
{
cutname = ajStrNew();
ajStrAssignS(&cutname, code);
ajListstrPushAppend(cutlist, cutname);
}
ajStrTokenDel(&tok);
}
ajStrDel(&code);
AJFREE(keyarray);
AJFREE(valarray);
/*
** Read in list of enzymes ('nocutlist') - either all or
** the input enzyme list.
** Exclude those that don't match the selection criteria - count these.
*/
ajDebug("Read in a list of all input enzyme names\n");
ne = 0;
if(!enzymes)
isall = ajTrue;
else
{
/* get input list of enzymes into ea[] */
ne = ajArrCommaList(enzymes, &ea);
if(ajStrMatchCaseC(ea[0], "all"))
isall = ajTrue;
else
{
isall = ajFalse;
for(i=0; i<ne; ++i)
ajStrRemoveWhite(&ea[i]);
}
}
enzfile = ajDatafileNewInNameC(ENZDATA);
/* push all enzyme names without the required criteria onto nocutlist */
enz = embPatRestrictNew();
while(!ajFileIsEof(enzfile))
{
if(!embPatRestrictReadEntry(enz, enzfile))
continue;
/*
** If user entered explicit enzyme list, then check to see if
** this is one of that explicit list
*/
if(!isall)
{
found = AJFALSE;
for(i=0; i<ne; ++i)
if(ajStrMatchCaseS(ea[i], enz->cod))
{
found = AJTRUE;
break;
}
if(!found) /* not in the explicit list */
continue;
ajDebug("RE %S is in the input explicit list of REs\n", enz->cod);
}
/* ignore ncuts==0 as they are unknown */
if(!enz->ncuts)
{
/* number of cut positions */
ajDebug("RE %S has an unknown number of cut positions\n",
enz->cod);
continue;
}
ajDebug("RE %S has a known number of cut sites\n", enz->cod);
if(enz->len < sitelen)
{
/* recognition site length */
ajDebug("RE %S does not have a long enough recognition site\n",
enz->cod);
rejected_count++;
continue;
}
if(!blunt && enz->blunt)
{
/* blunt/sticky */
ajDebug("RE %S is blunt\n", enz->cod);
rejected_count++;
continue;
}
if(!sticky && !enz->blunt)
{
/* blunt/sticky */
ajDebug("RE %S is sticky\n", enz->cod);
rejected_count++;
continue;
}
/* commercially available enzymes have uppercase patterns */
p = ajStrGetPtr(enz->pat);
/*
** The -commercial qualifier is only used if we are searching
** through 'all' of the REBASE database - if we have specified an
** explicit list of enzymes then they are searched for whether or
** not they are commercially available
*/
if((*p >= 'a' && *p <= 'z') && commercial && isall)
{
ajDebug("RE %S is not commercial\n", enz->cod);
rejected_count++;
continue;
}
if(!ambiguity && remap_Ambiguous(enz->pat)) {
ajDebug("RE %S is ambiguous\n", enz->cod);
rejected_count++;
continue;
}
ajDebug("RE %S matches all required criteria\n", enz->cod);
code = ajStrNew();
ajStrAssignS(&code, enz->cod);
ajListstrPushAppend(nocutlist, code);
}
embPatRestrictDel(&enz);
ajFileClose(&enzfile);
for(i=0; i<ne; ++i)
if(ea[i])
ajStrDel(&ea[i]);
if(ne)
AJFREE(ea);
/*
** Change names of enzymes in the non-cutter list
** to that of preferred (prototype)
** enzyme name so that the isoschizomers of cutters
** will be removed from the
** non-cutter list in the next bit.
** Remove duplicate prototype names.
*/
if(limit)
{
newlist = ajListstrNew();
remap_RenamePreferred(nocutlist, retable, newlist);
ajListstrFreeData(&nocutlist);
nocutlist = newlist;
ajListSortUnique(nocutlist, remap_cmpcase, remap_strdel);
}
/*
** Iterate through the list of input enzymes removing those that are in
** the cutlist.
*/
ajDebug("Remove from the nocutlist all enzymes and isoschizomers "
"that cut\n");
/*
** This steps down both lists at the same time, comparing names and
** iterating to the next name in whichever list whose name compares
** alphabetically before the other. Where a match is found, the
** nocutlist item is deleted.
*/
ajListSort(nocutlist, remap_cmpcase);
ajListSort(cutlist, remap_cmpcase);
citer = ajListIterNewread(cutlist);
niter = ajListIterNew(nocutlist);
/*
while((cutname = (AjPStr)ajListIterGet(citer)) != NULL)
ajDebug("dbg cutname = %S\n", cutname);
*/
nocutname = (AjPStr)ajListIterGet(niter);
cutname = (AjPStr)ajListIterGet(citer);
ajDebug("initial cutname, nocutname: '%S' '%S'\n", cutname, nocutname);
while(nocutname != NULL && cutname != NULL)
{
i = ajStrCmpCaseS(cutname, nocutname);
ajDebug("compare cutname, nocutname: %S %S ", cutname, nocutname);
ajDebug("ajStrCmpCase=%d\n", i);
if(i == 0)
{
/* match - so remove from nocutlist */
ajDebug("ajListstrRemove %S\n", nocutname);
ajListstrIterRemove(niter);
nocutname = (AjPStr)ajListIterGet(niter);
/*
** Don't increment the cutname list pointer here
** - there may be more than one entry in the nocutname
** list with the same name because we have converted
** isoschizomers to their preferred name
*/
/* cutname = (AjPStr)ajListIterGet(citer); */
}
else if(i == -1)
/* cutlist name sorts before nocutlist name */
cutname = (AjPStr)ajListIterGet(citer);
else if(i == 1)
/* nocutlist name sorts before cutlist name */
nocutname = (AjPStr)ajListIterGet(niter);
}
ajListIterDel(&citer);
ajListIterDel(&niter);
ajListstrFreeData(&cutlist);
/* Print the resulting list of those that do not cut*/
ajDebug("Print out the list\n");
/* print the title */
if(html)
ajFmtPrintF(outfile, "<H2>");
ajFmtPrintF(outfile, "\n\n# Enzymes that do not cut\n\n");
if(html)
ajFmtPrintF(outfile, "</H2>\n");
if(html)
ajFmtPrintF(outfile, "<PRE>");
/* ajListSort(nocutlist, ajStrVcmp);*/
niter = ajListIterNewread(nocutlist);
i = 0;
while((nocutname = (AjPStr)ajListIterGet(niter)) != NULL)
{
ajFmtPrintF(outfile, "%-10S", nocutname);
/* new line after every 7 names printed */
if(i++ == 7)
{
ajFmtPrintF(outfile, "\n");
i = 0;
}
}
ajListIterDel(&niter);
/* end the output */
ajFmtPrintF(outfile, "\n");
if(html) {ajFmtPrintF(outfile, "</PRE>\n");}
/*
** Print the count of rejected enzymes
** N.B. This is the count of ALL rejected enzymes including all
** isoschizomers
*/
if(html)
ajFmtPrintF(outfile, "<H2>");
ajFmtPrintF(outfile,
"\n\n# No. of cutting enzymes which do not match the\n"
"# SITELEN, BLUNT, STICKY, COMMERCIAL, AMBIGUOUS criteria\n\n");
if(html)
ajFmtPrintF(outfile, "</H2>\n");
ajFmtPrintF(outfile, "%d\n", rejected_count);
ajDebug("Tidy up\n");
ajListstrFreeData(&nocutlist);
ajListstrFreeData(&cutlist);
return;
}
static void remap_RestrictPreferred(const AjPList l, const AjPTable t)
{
AjIList iter = NULL;
EmbPMatMatch m = NULL;
const AjPStr value = NULL;
AjPStr newiso = NULL;
AjBool found; /* name found in isoschizomer list */
/* for parsing value->iso string */
AjPStrTok tok = NULL;
char tokens[] = " ,";
AjPStr code = NULL;
iter = ajListIterNewread(l);
while((m = (EmbPMatMatch)ajListIterGet(iter)))
{
found = ajFalse;
/* get prototype name */
value = ajTableFetchS(t, m->cod);
if(value)
{
ajStrAssignC(&newiso, "");
/* parse isoschizomer names from m->iso */
ajStrTokenDel(&tok);
tok = ajStrTokenNewC(m->iso, tokens);
while(ajStrTokenNextParseC(&tok, tokens, &code))
{
if(ajStrGetLen(newiso) > 0)
ajStrAppendC(&newiso, ",");
/* found the prototype name? */
if(!ajStrCmpCaseS(code, value))
{
ajStrAppendS(&newiso, m->cod);
found = ajTrue;
}
else
ajStrAppendS(&newiso, code);
}
ajStrTokenDel(&tok);
/* if the name was not replaced, then add it in now */
if(!found)
{
if(ajStrGetLen(newiso) > 0)
ajStrAppendC(&newiso, ",");
ajStrAppendS(&newiso, m->cod);
}
ajDebug("RE: %S -> %S iso=%S newiso=%S\n", m->cod, value,
m->iso, newiso);
/* replace the old iso string with the new one */
ajStrAssignS(&m->iso, newiso);
/* rename the enzyme to the prototype name */
ajStrAssignS(&m->cod, value);
}
}
ajListIterDel(&iter);
ajStrDel(&newiso);
ajStrDel(&code);
ajStrTokenDel(&tok);
return;
}
int main(int argc, char *argv[])
{
embInitPV("gseqinfo", argc, argv, "GEMBASSY", "1.0.1");
struct soap soap;
AjPSeqall seqall;
AjPSeq seq;
AjPStr inseq = NULL;
AjPStr seqid = NULL;
AjPStr tmp = NULL;
AjPStr parse = NULL;
AjPStr numA = NULL;
AjPStr numT = NULL;
AjPStr numG = NULL;
AjPStr numC = NULL;
AjPStrTok handle = NULL;
ajint n;
char *in0;
char *result;
AjBool show = 0;
AjPFile outf = NULL;
seqall = ajAcdGetSeqall("sequence");
outf = ajAcdGetOutfile("outfile");
while(ajSeqallNext(seqall, &seq))
{
soap_init(&soap);
inseq = NULL;
ajStrAppendC(&inseq, ">");
ajStrAppendS(&inseq, ajSeqGetNameS(seq));
ajStrAppendC(&inseq, "\n");
ajStrAppendS(&inseq, ajSeqGetSeqS(seq));
ajStrAssignS(&seqid, ajSeqGetAccS(seq));
in0 = ajCharNewS(inseq);
if(soap_call_ns1__seqinfo(
&soap,
NULL,
NULL,
in0,
&result
) == SOAP_OK)
{
tmp = ajStrNewC(result);
ajStrExchangeCC(&tmp, "<", "\n");
ajStrExchangeCC(&tmp, ">", "\n");
handle = ajStrTokenNewC(tmp, "\n");
while(ajStrTokenNextParse(handle, &parse))
{
if(ajStrIsInt(parse))
if(!numA)
numA = ajStrNewS(parse);
else if(!numT)
numT = ajStrNewS(parse);
else if(!numG)
numG = ajStrNewS(parse);
else if(!numC)
numC = ajStrNewS(parse);
}
if(show)
ajFmtPrint("Sequence: %S A: %S T: %S G: %S C: %S\n",
seqid, numA, numT, numG, numC);
else
ajFmtPrintF(outf, "Sequence: %S A: %S T: %S G: %S C: %S\n",
seqid, numA, numT, numG, numC);
}
else
{
soap_print_fault(&soap, stderr);
}
soap_destroy(&soap);
soap_end(&soap);
soap_done(&soap);
AJFREE(in0);
ajStrDel(&inseq);
}
ajSeqallDel(&seqall);
ajSeqDel(&seq);
ajStrDel(&seqid);
embExit();
return 0;
}
static AjBool extractfeat_MatchFeature(const AjPFeature gf,
const AjPStr source,
const AjPStr type, ajint sense,
AjBool testscore, float minscore,
float maxscore, const AjPStr tag,
const AjPStr value, AjBool *tagsmatch)
{
AjPStrTok tokens = NULL;
AjPStr key = NULL;
AjBool val = ajFalse;
/*
** is this a child of a join() ?
** if it is a child, then we use the previous result of MatchPatternTags
*/
if(!ajFeatIsMultiple(gf) || !ajFeatIsChild(gf))
*tagsmatch = extractfeat_MatchPatternTags(gf, tag, value);
/* ignore remote IDs */
/*
** No - don't reject remote IDs here. We want to include all features
** in this routine so that we can reject a whole join later on if any one
** member of the join is remote.
** if(!ajFeatIsLocal(gf))
** return ajFalse;
*/
/* check source, type, sense, score, tags, values */
/* Special values indication that we match anything:
** for strings, '*'
** for sense, 0
** for score, maxscore <= minscore
*/
ajDebug("extractfeat_MatchFeature\n");
ajDebug("embMiscMatchPatternDelim(ajFeatGetSource(gf), source) %B\n",
embMiscMatchPatternDelimC(ajFeatGetSource(gf), source, ",;|"));
ajDebug("ajFeatTypeMatchS(gf, type) %B\n",
ajFeatTypeMatchWildS(gf, type));
ajDebug("ajFeatGetStrand(gf) '%x' sense %d\n", ajFeatGetStrand(gf), sense);
ajDebug("testscore: %B ajFeatGetScore(gf): %f minscore:%f maxscore:%f\n",
testscore, ajFeatGetScore(gf), minscore, maxscore);
if(!embMiscMatchPatternDelimC(ajFeatGetSource(gf), source, ",;|"))
return ajFalse;
if(ajStrGetLen(type))
{
val = ajFalse;
tokens = ajStrTokenNewC(type, " \t\n\r,;|");
while (ajStrTokenNextParse( &tokens, &key))
{
if (ajFeatTypeMatchWildS(gf, key))
{
val = ajTrue;
break;
}
}
ajStrTokenDel( &tokens);
ajStrDel(&key);
if(!val)
return ajFalse;
}
if(ajFeatGetStrand(gf) == '+' && sense == -1)
return ajFalse;
if(ajFeatGetStrand(gf) == '-' && sense == +1)
return ajFalse;
if(testscore && ajFeatGetScore(gf) < minscore)
return ajFalse;
if(testscore && ajFeatGetScore(gf) > maxscore)
return ajFalse;
if(!*tagsmatch)
return ajFalse;
ajDebug("All tests passed, return ajTrue\n");
/*ajUser("All tests passed, return ajTrue");*/
return ajTrue;
}
int main(int argc, char *argv[])
{
embInitPV("ggcsi", argc, argv, "GEMBASSY", "1.0.1");
struct soap soap;
struct ns1__gcsiInputParams params;
AjPSeqall seqall;
AjPSeq seq;
AjPStr inseq = NULL;
AjPStr seqid = NULL;
ajint window = 0;
AjBool at = 0;
AjBool purine = 0;
AjBool keto = 0;
AjBool pval = 0;
AjPStr version = NULL;
AjBool accid = ajFalse;
AjPStr tmp = NULL;
AjPStr parse = NULL;
AjPStr gcsi = NULL;
AjPStr sa = NULL;
AjPStr dist = NULL;
AjPStr z = NULL;
AjPStr p = NULL;
AjPStrTok handle = NULL;
char *in0;
char *result;
AjPFile outf = NULL;
seqall = ajAcdGetSeqall("sequence");
window = ajAcdGetInt("window");
at = ajAcdGetBoolean("at");
purine = ajAcdGetBoolean("purine");
keto = ajAcdGetBoolean("keto");
pval = ajAcdGetBoolean("pval");
version = ajAcdGetSelectSingle("gcsi");
accid = ajAcdGetBoolean("accid");
outf = ajAcdGetOutfile("outfile");
params.window = window;
params.at = 0;
params.purine = 0;
params.keto = 0;
params.p = 0;
ajStrToInt(version, &(params.version));
if(at)
params.at = 1;
if(purine)
params.purine = 1;
if(keto)
params.keto = 1;
if(pval)
params.p = 1;
while(ajSeqallNext(seqall, &seq))
{
soap_init(&soap);
inseq = NULL;
ajStrAppendC(&inseq, ">");
ajStrAppendS(&inseq, ajSeqGetNameS(seq));
ajStrAppendC(&inseq, "\n");
ajStrAppendS(&inseq, ajSeqGetSeqS(seq));
ajStrAssignS(&seqid, ajSeqGetAccS(seq));
in0 = ajCharNewS(inseq);
if (soap_call_ns1__gcsi(
&soap,
NULL,
NULL,
in0,
¶ms,
&result
) == SOAP_OK)
{
tmp = ajStrNew();
parse = ajStrNew();
gcsi = ajStrNew();
sa = ajStrNew();
dist = ajStrNew();
z = ajStrNew();
p = ajStrNew();
ajStrAssignC(&tmp, result);
ajStrExchangeCC(&tmp, "<", "\n");
ajStrExchangeCC(&tmp, ">", "\n");
handle = ajStrTokenNewC(tmp, "\n");
while (ajStrTokenNextParse(&handle, &parse))
{
if (ajStrIsFloat(parse))
{
if(!ajStrGetLen(gcsi))
ajStrAssignS(&gcsi, parse);
else if(!ajStrGetLen(sa))
ajStrAssignS(&sa, parse);
else if(!ajStrGetLen(dist))
ajStrAssignS(&dist, parse);
else if(!ajStrGetLen(z))
ajStrAssignS(&z, parse);
else if(!ajStrGetLen(p))
ajStrAssignS(&p, parse);
}
}
tmp = ajFmtStr("Sequence: %S GCSI: %S SA: %S DIST: %S",
seqid, gcsi, sa, dist);
if(pval)
tmp = ajFmtStr("%S Z: %S P: %S", tmp, z, p);
ajFmtPrintF(outf, "%S\n", tmp);
ajStrDel(&tmp);
ajStrDel(&parse);
ajStrDel(&gcsi);
ajStrDel(&sa);
ajStrDel(&dist);
ajStrDel(&z);
ajStrDel(&p);
}
else
{
soap_print_fault(&soap, stderr);
}
soap_destroy(&soap);
soap_end(&soap);
soap_done(&soap);
AJFREE(in0);
ajStrDel(&inseq);
}
ajFileClose(&outf);
ajSeqallDel(&seqall);
ajSeqDel(&seq);
ajStrDel(&seqid);
embExit();
return 0;
}
static void primersearch_read_primers(AjPList *primerList, AjPFile primerFile,
ajint mmp)
{
AjPStr rdline = NULL;
AjPStrTok handle = NULL;
ajint nprimers = 0;
Primer primdata = NULL;
while (ajReadlineTrim(primerFile, &rdline))
{
primdata = NULL;
if (ajStrGetCharFirst(rdline) == '#')
continue;
if (ajStrSuffixC(rdline, ".."))
continue;
AJNEW(primdata);
primdata->Name = NULL;
primersearch_initialise_pguts(&primdata->forward);
primersearch_initialise_pguts(&primdata->reverse);
primdata->hitlist = ajListNew();
handle = ajStrTokenNewC(rdline, " \t");
ajStrTokenNextParse(&handle, &primdata->Name);
ajStrTokenNextParse(&handle, &primdata->forward->patstr);
ajStrFmtUpper(&primdata->forward->patstr);
ajStrTokenNextParse(&handle, &primdata->reverse->patstr);
ajStrFmtUpper(&primdata->reverse->patstr);
ajStrTokenDel(&handle);
/* copy patterns for Henry Spencer code */
ajStrAssignC(&primdata->forward->origpat,
ajStrGetPtr(primdata->forward->patstr));
ajStrAssignC(&primdata->reverse->origpat,
ajStrGetPtr(primdata->reverse->patstr));
/* set the mismatch level */
primdata->forward->mm = (ajint)
(ajStrGetLen(primdata->forward->patstr)*mmp)/100;
primdata->reverse->mm = (ajint)
(ajStrGetLen(primdata->reverse->patstr)*mmp)/100;
if(primersearch_classify_and_compile(&primdata))
{
ajListPushAppend(*primerList, primdata);
nprimers++;
}
else /* there was something funny about the primer sequences */
{
ajUser("Cannot use %s\n", ajStrGetPtr(primdata->Name));
primersearch_free_pguts(&primdata->forward);
primersearch_free_pguts(&primdata->reverse);
ajStrDel(&primdata->Name);
ajListFree(&primdata->hitlist);
ajListFree(&primdata->hitlist);
AJFREE(primdata);
}
}
ajStrDel(&rdline);
return;
}
int main(int argc, char *argv[])
{
embInitPV("genret", argc, argv, "GEMBASSY", "1.0.3");
AjPSeqall seqall;
AjPSeq seq = NULL;
AjPStr inseq = NULL;
AjPStr gene = NULL;
AjPStr access = NULL;
AjBool accid = ajTrue;
AjPStr argument = NULL;
AjPFile outfile = NULL;
AjPStr seqid = NULL;
AjPStr restid = NULL;
AjBool valid = ajFalse;
AjBool isseq = ajFalse;
AjBool isgbk = ajFalse;
AjPFilebuff buff = NULL;
AjPFile tmpfile = NULL;
AjPStr tmpname = NULL;
AjPStr regexstr = NULL;
AjPStrTok token = NULL;
AjPRegexp regex = NULL;
AjPStr url = NULL;
AjPStr base = NULL;
AjPStr head = NULL;
AjPStr line = NULL;
seqall = ajAcdGetSeqall("sequence");
access = ajAcdGetString("access");
gene = ajAcdGetString("gene");
argument = ajAcdGetString("argument");
accid = ajAcdGetBoolean("accid");
outfile = ajAcdGetOutfile("outfile");
if(
ajStrMatchC(access, "translation") ||
ajStrMatchC(access, "get_exon") ||
ajStrMatchC(access, "get_exons") ||
ajStrMatchC(access, "get_cdsseq") ||
ajStrMatchC(access, "get_gbkseq") ||
ajStrMatchC(access, "get_geneseq") ||
ajStrMatchC(access, "get_intron") ||
ajStrMatchC(access, "getseq") ||
ajStrMatchC(access, "seq") ||
ajStrMatchC(access, "around_startcodon") ||
ajStrMatchC(access, "around_stopcodon") ||
ajStrMatchC(access, "before_startcodon") ||
ajStrMatchC(access, "before_stopcodon") ||
ajStrMatchC(access, "after_startcodon") ||
ajStrMatchC(access, "after_stopcodon")
)
{
isseq = ajTrue;
}
else if(ajStrMatchC(access, "annotate") ||
ajStrMatchC(access, "output"))
{
isgbk = ajTrue;
}
else
{
ajFmtPrintF(outfile, "gene,%S\n", access);
}
base = ajStrNewC("rest.g-language.org");
ajStrExchangeCC(&argument, " ", "/");
ajStrExchangeCC(&argument, ",", "/");
ajStrExchangeCC(&argument, "\t", "/");
ajStrExchangeCC(&argument, "\r", "/");
ajStrExchangeCC(&argument, "\n", "/");
if(ajStrMatchC(gene, "*"))
{
ajStrInsertK(&gene, 0, '.');
}
if(ajStrPrefixC(gene, "@") || ajStrPrefixC(gene, "list::"))
{
ajStrExchangeCC(&gene, "@", "");
ajStrExchangeCC(&gene, "list::", "");
ajStrAssignS(&tmpname, gene);
tmpfile = ajFileNewInNameS(tmpname);
if(!tmpfile)
{
ajDie("List file (%S) open error\n", tmpname);
}
gene = ajStrNew();
while(ajReadline(tmpfile, &line))
{
ajStrAppendS(&gene, line);
}
ajFileClose(&tmpfile);
ajStrDel(&tmpname);
ajStrDel(&line);
}
tmpname = ajStrNew();
gAssignUniqueName(&tmpname);
while(ajSeqallNext(seqall, &seq))
{
inseq = ajStrNew();
if(!accid)
{
if(gFormatGenbank(seq, &inseq))
{
tmpfile = ajFileNewOutNameS(tmpname);
if(!tmpfile)
{
ajDie("Output file (%S) open error\n", tmpname);
}
ajFmtPrintF(tmpfile, "%S", inseq);
ajFileClose(&tmpfile);
ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
gFilePostSS(url, tmpname, &restid);
ajStrDel(&url);
ajSysFileUnlinkS(tmpname);
}
else
{
ajWarn("Sequence does not have features\n"
"Proceeding with sequence accession ID\n");
accid = ajTrue;
}
}
ajStrAssignS(&seqid, ajSeqGetAccS(seq));
if(ajStrGetLen(seqid) == 0)
{
ajStrAssignS(&seqid, ajSeqGetNameS(seq));
}
if(ajStrGetLen(seqid) == 0)
{
ajWarn("No valid header information\n");
}
if(accid)
{
ajStrAssignS(&restid, seqid);
if(ajStrGetLen(seqid) == 0)
{
ajDie("Cannot proceed without header with -accid\n");
}
if(!gValID(seqid))
{
ajDie("Invalid accession ID:%S, exiting\n", seqid);
}
}
url = ajStrNew();
if(isgbk)
{
ajFmtPrintS(&url, "http://%S/%S/%S", base, restid, access);
}
else
{
ajFmtPrintS(&url, "http://%S/%S/*/%S/%S", base, restid, access, argument);
}
if(!gFilebuffURLS(url, &buff))
{
ajDie("GET error from %S\n", url);
}
while(ajBuffreadLine(buff, &line))
{
if(isgbk){
ajFmtPrintF(outfile, "%S", line);
continue;
}
ajStrRemoveLastNewline(&line);
regex = ajRegCompC("^>");
if(ajRegExec(regex, line))
{
head = ajStrNew();
ajStrAssignS(&head, line);
ajStrTrimStartC(&head, ">");
valid = ajFalse;
token = ajStrTokenNewC(ajStrNewS(gene), " ,\t\r\n");
while(ajStrTokenNextParse(token, ®exstr))
{
if(ajStrGetLen(regexstr))
{
regex = ajRegComp(regexstr);
if(ajRegExec(regex, line))
{
valid = ajTrue;
if(ajStrIsAlnum(regexstr))
{
ajStrExchangeSC(&gene, regexstr, "");
}
}
ajRegFree(®ex);
}
}
}
else
{
if(valid)
{
if(isseq)
{
ajStrFmtWrap(&line, 60);
ajFmtPrintF(outfile, ">%S\n%S\n", head, line);
}
else
{
ajFmtPrintF(outfile, "%S,%S\n", head, line);
}
valid = ajFalse;
}
}
}
ajFileClose(&outfile);
ajStrDel(&restid);
ajStrDel(&seqid);
ajStrDel(&inseq);
}
ajSeqallDel(&seqall);
ajSeqDel(&seq);
ajStrDel(&access);
ajStrDel(&gene);
embExit();
}
static void eprimer3_report(AjPFile outfile, const AjPStr output,
ajint numreturn, ajint begin)
{
AjPStr line = NULL;
AjPStrTok linetokenhandle;
char eol[] = "\n\r";
AjPStrTok keytokenhandle;
char equals[] = "=";
AjPStr key = NULL;
AjPStr value = NULL;
AjBool gotsequenceid = AJFALSE;
AjPTable table;
linetokenhandle = ajStrTokenNewC(output, eol);
/* get next line of relevant results */
while(ajStrTokenNextParseC(&linetokenhandle, eol, &line))
{
if(!gotsequenceid)
{
/*
** Att the start of another sequence's results?
** Start storing the results in the table.
*/
if(ajStrCmpLenC(line, "PRIMER_SEQUENCE_ID=", 19) == 0)
{
gotsequenceid = AJTRUE;
table = ajTablestrNew(TABLEGUESS);
}
else
continue;
}
else
{
/*
** At the end of this sequence? - marked by a '=' in the primer3
** output - then output the results.
*/
if(ajStrCmpC(line, "=") == 0)
{
gotsequenceid = AJFALSE;
eprimer3_output_report(outfile, table, numreturn, begin);
ajTablestrFree(&table);
continue;
}
}
/*
** store key and value in table and parse values
** when have all of the sequence
** results in the table because the LEFT, RIGHT
** and INTERNAL results for each
** resulting primer are interleaved
*/
keytokenhandle = ajStrTokenNewC(line, equals);
key = ajStrNew();
ajStrTokenNextParse(&keytokenhandle, &key);
value = ajStrNew();
ajStrTokenNextParse(&keytokenhandle, &value);
ajDebug("key=%S\tvalue=%S\n", key, value);
ajTablePut(table,(void *)key, (void *)value);
ajStrTokenDel(&keytokenhandle);
}
ajStrDel(&line);
ajStrTokenDel(&linetokenhandle);
ajTablestrFree(&table);
return;
}
int main(int argc, char **argv)
{
AjPSeqall seqall;
AjPFile primfile;
AjPStr rdline = NULL;
Primer primdata;
AjPStrTok handle = NULL;
AjPList primList = NULL;
embInit("stssearch", argc, argv);
primfile = ajAcdGetInfile("infile");
out = ajAcdGetOutfile("outfile");
seqall = ajAcdGetSeqall("seqall");
while(ajReadlineTrim(primfile, &rdline))
{
if(ajStrGetCharFirst(rdline) == '#')
continue;
if(ajStrSuffixC(rdline, ".."))
continue;
AJNEW(primdata);
primdata->Name = NULL;
primdata->Oligoa = NULL;
primdata->Oligob = NULL;
handle = ajStrTokenNewC(rdline, " \t");
ajStrTokenNextParse(&handle, &primdata->Name);
if(!(nprimers % 1000))
ajDebug("Name [%d]: '%S'\n", nprimers, primdata->Name);
ajStrTokenNextParse(&handle, &primdata->Oligoa);
ajStrFmtUpper(&primdata->Oligoa);
primdata->Prima = ajRegComp(primdata->Oligoa);
ajStrTokenNextParse(&handle, &primdata->Oligob);
ajStrFmtUpper(&primdata->Oligob);
primdata->Primb = ajRegComp(primdata->Oligob);
ajStrTokenDel(&handle);
if(!nprimers)
primList = ajListNew();
ajListPushAppend(primList, primdata);
nprimers++;
}
if(!nprimers)
ajFatal("No primers read\n");
ajDebug("%d primers read\n", nprimers);
while(ajSeqallNext(seqall, &seq))
{
ajSeqFmtUpper(seq);
ajStrAssignS(&seqstr, ajSeqGetSeqS(seq));
ajStrAssignS(&revstr, ajSeqGetSeqS(seq));
ajSeqstrReverse(&revstr);
ajDebug("Testing: %s\n", ajSeqGetNameC(seq));
ntests = 0;
ajListMap(primList, stssearch_primTest, NULL);
}
ajFileClose(&out);
ajSeqallDel(&seqall);
ajSeqDel(&seq);
ajFileClose(&out);
ajStrDel(&revstr);
ajStrDel(&seqstr);
ajFileClose(&primfile);
ajListMap(primList, stssearch_primDel, NULL);
ajListFree(&primList);
ajStrDel(&rdline);
embExit();
return 0;
}
static char* cutgextract_next(AjPFile inf, const AjPStr wildspecies,
AjPStr* pspecies, AjPStr* pdoc)
{
AjPStrTok handle = NULL;
AjPStr token = NULL;
ajint i;
ajint len;
char *p = NULL;
char c;
AjBool done = ajFalse;
if(!cutgextractLine)
cutgextractLine = ajStrNew();
if(!cutgextractOrg)
cutgextractOrg = ajStrNew();
ajStrAssignC(&cutgextractLine,"");
ajStrAssignC(pdoc,"");
while (!done)
{
while(ajStrGetCharFirst(cutgextractLine) != '>')
if(!ajReadlineTrim(inf,&cutgextractLine))
return NULL;
handle = ajStrTokenNewC(cutgextractLine,"\\\n\t\r");
for(i=0;i<7;++i) {
ajStrTokenNextParseC(&handle,"\\\n\t\r",&token);
if(i==5)
{
ajStrAssignC(&cutgextractOrg,"E");
ajStrAppendS(&cutgextractOrg, token);
ajStrAssignS(pspecies, token);
if(ajStrMatchWildS(token,wildspecies))
{
done = ajTrue;
}
}
switch(i)
{
case 0:
ajStrAppendC(pdoc, "#ID ");
ajStrAppendS(pdoc, token);
ajStrAppendC(pdoc, "\n");
break;
case 1:
ajStrAppendC(pdoc, "#AC ");
ajStrAppendS(pdoc, token);
ajStrAppendC(pdoc, "\n");
break;
case 2:
ajStrAppendC(pdoc, "#FT ");
ajStrAppendS(pdoc, token);
ajStrAppendC(pdoc, "\n");
break;
case 3:
ajStrAppendC(pdoc, "#FL ");
ajStrAppendS(pdoc, token);
ajStrAppendC(pdoc, "\n");
break;
case 4:
ajStrAppendC(pdoc, "#PI ");
ajStrAppendS(pdoc, token);
ajStrAppendC(pdoc, "\n");
ajStrAssignS(&cutgextractSavepid, token);
break;
case 5:
ajStrAppendC(pdoc, "#OS ");
ajStrAppendS(pdoc, token);
ajStrAppendC(pdoc, "\n");
break;
case 6:
ajStrAppendC(pdoc, "#DE ");
ajStrAppendS(pdoc, token);
ajStrAppendC(pdoc, "\n");
break;
default:
break;
}
}
ajStrTokenDel(&handle);
ajStrDel(&token);
if(!done)
if(!ajReadlineTrim(inf,&cutgextractLine))
return NULL;
}
len = ajStrGetLen(cutgextractOrg);
p = ajStrGetuniquePtr(&cutgextractOrg);
for(i=0;i<len;++i)
{
c = p[i];
if(c=='/' || c==' ' || c=='.' || c=='\'')
p[i]='_';
}
if(p[strlen(p)-1]=='_')
p[strlen(p)-1]='\0';
ajStrDel(&token);
ajStrTokenDel(&handle);
return p;
}
int main(int argc, char **argv)
{
/* Variable declarations */
AjPStr query;
AjPOutfile outfile = NULL;
AjPResource resource = NULL;
AjPResourcein resourcein = NULL;
AjPOboin oboin = NULL;
AjPObo obo = NULL;
AjPStr oboqry = NULL;
AjPStr resourceqry = NULL;
AjPStr qrystr = NULL;
AjPTable obotable = NULL;
AjPTable foundtable = NULL;
AjBool subclasses = ajFalse;
AjPStrTok handle = NULL;
AjPList obolist = NULL;
AjPObo obotest = NULL;
ajuint i;
ajuint imax = 3;
const char* fields[] = {"id", "acc", "nam", "des"};
/* ACD processing */
embInit("drfindid", argc, argv);
query = ajAcdGetString("query");
outfile = ajAcdGetOutresource("outfile");
/* sensitive = ajAcdGetBoolean("sensitive"); */
subclasses = ajAcdGetBoolean("subclasses");
resourcein = ajResourceinNew();
resource = ajResourceNew();
oboin = ajOboinNew();
obo = ajOboNew();
obolist = ajListNew();
obotable = ajTablestrNew(600);
foundtable = ajTablestrNew(600);
handle = ajStrTokenNewC(query, ",");
while(ajStrTokenNextParse(&handle, &qrystr))
{
for(i=0; i<imax; i++)
{
ajFmtPrintS(&oboqry, "edam-%s:%S", fields[i], qrystr);
ajOboinQryS(oboin, oboqry);
while(ajOboinRead(oboin, obo))
{
ajListPushAppend(obolist, ajOboNewObo(obo));
if(subclasses)
ajOboGetTree(obo, obolist);
ajDebug("%S '%S' %u\n",
qrystr, obo->Id, ajListGetLength(obolist));
while(ajListGetLength(obolist))
{
ajListPop(obolist, (void**) &obotest);
if(!ajTableMatchS(obotable, obotest->Id))
{
ajDebug("edam %s '%S' namespace '%S' name '%S'\n",
fields[i], obotest->Id, obotest->Namespace,
obotest->Name);
ajTablePut(obotable, ajStrNewS(obotest->Id),
(void *) 1);
ajFmtPrintS(&resourceqry, "drcat-eid:%S",
ajOboGetId(obotest));
ajResourceinQryS(resourcein, resourceqry);
while(ajResourceinRead(resourcein, resource))
{
if(!ajTableMatchS(foundtable, resource->Id))
{
ajDebug("drcat id '%S' category '%S'\n",
resource->Id, resource->Cat);
ajResourceoutWrite(outfile, resource);
ajTablePut(foundtable, ajStrNewS(resource->Id),
(void *) 1);
}
}
}
ajOboDel(&obotest);
}
}
}
}
/* Memory clean-up and exit */
ajOboDel(&obo);
ajOboinDel(&oboin);
ajResourceDel(&resource);
ajResourceinDel(&resourcein);
ajListFree(&obolist);
ajStrTokenDel(&handle);
ajStrDel(&qrystr);
ajStrDel(&query);
ajStrDel(&oboqry);
ajStrDel(&resourceqry);
ajTablestrFreeKey(&obotable);
ajTablestrFreeKey(&foundtable);
ajOutfileClose(&outfile);
embExit();
return 0;
}
