pulsesequence() {
// Define Variables and Objects and Get Parameter Values
MPSEQ dec = getblew("blewH",0,0.0,0.0,0,1);
strncpy(dec.ch,"dec",3);
putCmd("chHblew='dec'\n");
CP hx = getcp("HX",0.0,0.0,0,1);
strncpy(hx.fr,"dec",3);
strncpy(hx.to,"obs",3);
putCmd("frHX='dec'\n");
putCmd("toHX='obs'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwH90") + getval("tHX") + getval("rd") + getval("ad") + at;
d.dutyoff = d1 + 4.0e-6;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phH90,4,table1);
settable(phXhx,4,table2);
settable(phHhx,4,table3);
settable(phRec,4,table4);
setreceiver(phRec);
// Begin Sequence
txphase(phXhx); decphase(phH90);
obspwrf(getval("aXhx")); decpwrf(getval("aH90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H to X Cross Polarization
decrgpulse(getval("pwH90"),phH90,0.0,0.0);
decphase(phHhx);
_cp_(hx,phHhx,phXhx);
// Begin Acquisition
_mpseqon(dec, phHhx);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_mpseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
void pulsesequence() {
// Define Variables and Objects and Get Parameter Values
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwX90");
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phX90,4,table1);
settable(phRec,4,table2);
setreceiver(phRec);
// Begin Sequence
txphase(phX90); decphase(zero);
obspwrf(getval("aX90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// X Direct Polarization
rgpulse(getval("pwX90"),phX90,0.0,0.0);
// Begin Acquisition
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
initval(getval("periods"),v2);
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Set Phase Tables
settable(phX90,4,table1);
settable(phRec,4,table2);
setreceiver(phRec);
// Begin Sequence
txphase(phX90); decphase(zero);
obspwrf(getval("aX90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
xgate(1.0);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// Apply a Rotorsync Delay
rgpulse(getval("pwX90"),phX90,0.0,0.0);
rotorsync(v2);
rgpulse(getval("pwX90"),phX90,0.0,0.0);
xgate(getval("xperiods"));
rgpulse(getval("pwX90"),phX90,0.0,0.0);
delay(10.0e-6);
// X Direct Polarization
rgpulse(getval("pwX90"),phX90,0.0,0.0);
// Begin Acquisition
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
obsunblank(); decunblank(); _unblank34();
}
void pulsesequence(){
//Define Variables and Get Parameter Values
double pwTune = getval("pwTune");
pwTune = pwTune*6.0;
at = pwTune*2.0;
char atval[MAXSTR];
sprintf(atval,"at = %f\n", at);
putCmd(atval);
int chTune = (int) getval("chTune");
if ((chTune < 1) || (chTune > 4)) {
abort_message("chTune(%d) must be between 1 and 4\n", chTune);
}
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Set Phase Tables
settable(phTune,4,table1);
settable(phRec,4,table2);
setreceiver(t2);
//Begin Sequence
obspwrf(getval("aTune"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2e-6); sp1off(); delay(2.0e-6);
//Begin Phase Detected Pulse
set4Tune(chTune,getval("gain"));
delay(1.0e-4);
ShapedXmtNAcquire("phtran",pwTune,phTune,6.0e-6,chTune);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
double pw1Xstmas = getval("pw1Xstmas");
double pw2Xstmas = getval("pw2Xstmas");
double tXzfselinit = getval("tXzfsel");
double tXzfsel = tXzfselinit - 3.0e-6;
if (tXzfsel < 0.0) tXzfsel = 0.0;
double d2init = getval("d2");
double d2 = d2init - pw1Xstmas/2.0 - pw2Xstmas/2.0;
if (d2 < 0.0) d2 = 0.0;
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
// Set Constant-time Period for d2.
if (d2_index == 0) d2_init = getval("d2");
double d2_ = (ni - 1)/sw1 + d2_init;
putCmd("d2acqret = %f\n",roundoff(d2_,12.5e-9));
putCmd("d2dwret = %f\n",roundoff(1.0/sw1,12.5e-9));
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pw1Xstmas") + getval("pw2Xstmas") + getval("pwXzfsel");
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = d2_ + tXzfsel + getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = d2_ + tXzfsel + getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(ph1Xstmas,4,table1);
settable(ph2Xstmas,4,table2);
settable(phXzfsel,8,table3);
settable(phRec,8,table4);
if (phase1 == 2) {
tsadd(ph1Xstmas,1,4);
}
setreceiver(phRec);
// Begin Sequence
txphase(ph1Xstmas); decphase(zero);
obspower(getval("tpwr"));
obspwrf(getval("aXstmas"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H Decoupler on Before STMAS
_dseqon(dec);
// Two-Pulse STMAS
rgpulse(getval("pw1Xstmas"),ph1Xstmas,0.0,0.0);
txphase(ph2Xstmas);
delay(d2);
rgpulse(getval("pw2Xstmas"),ph2Xstmas,0.0,0.0);
// Z-filter Pulse
txphase(phXzfsel);
obsblank();
obspower(getval("dbXzfsel"));
obspwrf(getval("aXzfsel"));
delay(3.0e-6);
obsunblank();
delay(tXzfsel);
rgpulse(getval("pwXzfsel"),phXzfsel,0.0,0.0);
// Begin Acquisition
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
double aXfam2 = getval("aXfam2");
double pw1Xfam2 = getval("pw1Xfam2");
double pw2Xfam2 = getval("pw2Xfam2");
double pw3Xfam2 = getval("pw3Xfam2");
double pw4Xfam2 = getval("pw4Xfam2");
double nXfam2 = getval("nXfam2");
initval(nXfam2,v4);
putCmd("pw2Xmqmas=pwXfam1"); // Sequence uses pwXfam1 and sets pw2Xmqmas
double d2init = getval("d2"); // Define the Split d2 in the Pulse Sequence
double ival = getval("ival");
double d20 = 1.0;
double d21 = 0.0;
double d22 = 0.0;
if (ival == 1.5) {
d20 = 9.0*d2init/16.0;
d21 = 7.0*d2init/16.0;
d22 = 0.0;
}
else if (ival == 2.5) {
d20 = 12.0*d2init/31.0;
d21 = 0.0*d2init/31.0;
d22 = 19.0*d2init/31.0;
}
else {
d20 = 1.0*d2init;
d21 = 0.0*d2init;
d22 = 0.0*d2init;
}
double tXechselinit = getval("tXechsel"); // Adjust the selective echo delay for the
double tXechsel = tXechselinit - 3.0e-6; // attenuator switch time.
if (tXechsel < 0.0) tXechsel = 0.0;
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
// Set Constant-time Period for d2.
if (d2_index == 0) d2_init = getval("d2");
double d2_ = (ni - 1)/sw1 + d2_init;
putCmd("d2acqret = %f\n",roundoff(d2_,12.5e-9));
putCmd("d2dwret = %f\n",roundoff(1.0/sw1,12.5e-9));
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pw1Xmqmas") + nXfam2*(pw1Xfam2 + pw2Xfam2 + pw3Xfam2 +pw4Xfam2) +
getval("pwXechsel");
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = d2_ + tXechselinit + getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = d2_ + tXechselinit + getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
if (phase1 == 0) {
settable(phf1Xmqmas,12,table1);
settable(ph1Xfam2,6,table2);
settable(ph2Xfam2,6,table3);
settable(phfXechsel,96,table4);
settable(phRec,48,table5);
}
else {
settable(phf1Xmqmas,6,table6);
settable(ph1Xfam2,6,table7);
settable(ph2Xfam2,6,table8);
settable(phfXechsel,48,table9);
settable(phRec,24,table10);
if (phase1 == 2) {
tsadd(phf1Xmqmas,30,360);
}
}
setreceiver(phRec);
obsstepsize(1.0);
// Begin Sequence
xmtrphase(phf1Xmqmas); decphase(zero);
obspower(getval("tpwr"));
obspwrf(getval("aXmqmas"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H Decoupler on Before MQMAS
_dseqon(dec);
// Two-Pulse MQMAS with DFS Conversion
rgpulse(getval("pw1Xmqmas"),zero,0.0,0.0);
xmtrphase(zero); txphase(ph1Xfam2);
obspwrf(aXfam2);
delay(d20);
// X FAM2 Pulse
loop(v4,v5);
xmtron();
delay(pw1Xfam2);
xmtroff();
txphase(ph2Xfam2);
delay(pw2Xfam2);
xmtron();
delay(pw3Xfam2);
xmtroff();
txphase(ph2Xfam2);
delay(pw4Xfam2);
endloop(v5);
// Tau Delay and Second Selective Echo Pulse
xmtrphase(phfXechsel);
obsblank();
obspower(getval("dbXechsel"));
obspwrf(getval("aXechsel"));
delay(3.0e-6);
obsunblank();
delay(d21 + tXechsel);
rgpulse(getval("pwXechsel"),zero,0.0,0.0);
delay(d22);
// Begin Acquisition
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence()
{
/* DECLARE VARIABLES */
char fsat[MAXSTR],
fscuba[MAXSTR],
f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
C_flg[MAXSTR],
dtt_flg[MAXSTR];
int phase, phase2, ni, ni2,
t1_counter, /* used for states tppi in t1 */
t2_counter; /* used for states tppi in t2 */
double tau1, /* t1 delay */
tau2, /* t2 delay */
taua, /* ~ 1/4JHC = 1.6 ms */
taub, /* 1/6JCH = 1.1 ms */
BigTC, /* Carbon constant time period = 1/4Jcc = 7.0 ms */
BigTC1, /* Carbon constant time period2 < 1/4Jcc to account for relaxation */
pwN, /* PW90 for 15N pulse @ pwNlvl */
pwC, /* PW90 for c nucleus @ pwClvl */
pwcrb180, /* PW180 for C 180 reburp @ rfrb */
pwClvl, /* power level for 13C pulses on dec1 */
compC, compH, /* compression factors for H1 and C13 amps */
rfrb, /* power level for 13C reburp pulse */
pwNlvl, /* high dec2 pwr for 15N hard pulses */
sw1, /* sweep width in f1 */
sw2, /* sweep width in f2 */
tofps, /* tof for presat */
gt0,
gt1,
gt2,
gt3,
gt4,
gstab,
gzlvl0,
gzlvl1,
gzlvl2,
gzlvl3,
gzlvl4,
decstep1,
bw, ofs, ppm,
pwd1,
dpwr3_D,
pwd,
tpwrs,
pwHs,
dof_me,
tof_dtt,
tpwrs1,
pwHs1,
dpwrsed,
pwsed,
dressed,
rfrb_cg,
pwrb_cg;
/* LOAD VARIABLES */
getstr("fsat",fsat);
getstr("f1180",f1180);
getstr("f2180",f2180);
getstr("fscuba",fscuba);
getstr("C_flg",C_flg);
getstr("dtt_flg",dtt_flg);
tofps = getval("tofps");
taua = getval("taua");
taub = getval("taub");
BigTC = getval("BigTC");
BigTC1 = getval("BigTC1");
pwC = getval("pwC");
pwcrb180 = getval("pwcrb180");
pwN = getval("pwN");
tpwr = getval("tpwr");
pwClvl = getval("pwClvl");
compC = getval("compC");
compH = getval("compH");
dpwr = getval("dpwr");
pwNlvl = getval("pwNlvl");
phase = (int) ( getval("phase") + 0.5);
phase2 = (int) ( getval("phase2") + 0.5);
sw1 = getval("sw1");
sw2 = getval("sw2");
ni = getval("ni");
ni2 = getval("ni2");
gt0 = getval("gt0");
gt1 = getval("gt1");
gt2 = getval("gt2");
gt3 = getval("gt3");
gt4 = getval("gt4");
gstab = getval("gstab");
gzlvl0 = getval("gzlvl0");
gzlvl1 = getval("gzlvl1");
gzlvl2 = getval("gzlvl2");
gzlvl3 = getval("gzlvl3");
gzlvl4 = getval("gzlvl4");
decstep1 = getval("decstep1");
pwd1 = getval("pwd1");
dpwr3_D = getval("dpwr3_D");
pwd = getval("pwd");
pwHs = getval("pwHs");
dof_me = getval("dof_me");
pwHs1 = pwHs;
tpwrs=-16.0; tpwrs1=tpwrs;
tof_dtt = getval("tof_dtt");
dpwrsed = -16;
pwsed = 1000.0;
dressed = 90.0;
pwrb_cg = 0.0;
setautocal(); /* activate auto-calibration */
if(FIRST_FID) /* make shapes */
{
ppm = getval("dfrq");
bw = 80.0*ppm;
rb180 = pbox_make("rb180P", "reburp", bw, 0.0, compC*pwC, pwClvl);
bw = 8.125*ppm; ofs = -24.0*ppm;
rb180_cg = pbox_make("rb180_cgP", "reburp", bw, ofs, compC*pwC, pwClvl);
bw = 20.0*ppm; ofs = 136.0*ppm;
cosed = pbox("COsedP", CODEC, CODECps, dfrq, compC*pwC, pwClvl);
if(taua < (gt4+106e-6+pwHs)) printf("gt4 or pwHs may be too long! ");
if(taub < rb180_cg.pw) printf("rb180_cgP pulse may be too long! ");
}
pwcrb180 = rb180.pw; rfrb = rb180.pwrf; /* set up parameters */
pwrb_cg = rb180_cg.pw; rfrb_cg = rb180_cg.pwrf; /* set up parameters */
tpwrs = tpwr - 20.0*log10(pwHs/((compH*pw)*1.69)); /* sinc=1.69xrect */
tpwrs = (int) (tpwrs); tpwrs1=tpwrs;
dpwrsed = cosed.pwr; pwsed = 1.0/cosed.dmf; dressed = cosed.dres;
/* LOAD PHASE TABLE */
settable(t1,2,phi1);
settable(t2,4,phi2);
settable(t3,4,phi3);
settable(t4,4,phi4);
settable(t5,8,phi5);
settable(t6,8,phi6);
settable(t7,8,phi7);
settable(t8,1,phi8);
settable(t9,2,rec);
/* CHECK VALIDITY OF PARAMETER RANGES */
if( BigTC - 0.5*(ni2-1)*1/(sw2) - WFG_STOP_DELAY - POWER_DELAY
- 4.0e-6
< 0.2e-6 )
{
printf(" ni2 is too big\n");
psg_abort(1);
}
if((dm[A] == 'y' || dm[B] == 'y' || dm[C] == 'y' ))
{
printf("incorrect dec1 decoupler flags! ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[B] == 'y' || dm2[C] == 'y' || dm2[D] == 'y'))
{
printf("incorrect dec2 decoupler flags! Should be 'nnnn' ");
psg_abort(1);
}
if( satpwr > 6 )
{
printf("SATPWR too large !!! ");
psg_abort(1);
}
if( dpwr > 48 )
{
printf("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if( dpwr2 > -16 )
{
printf("don't fry the probe, DPWR2 too large! ");
psg_abort(1);
}
if( pw > 200.0e-6 )
{
printf("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwN > 200.0e-6 )
{
printf("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
if( pwC > 200.0e-6 )
{
printf("dont fry the probe, pwC too high ! ");
psg_abort(1);
}
if( pwcrb180 > 500.0e-6 )
{
printf("dont fry the probe, pwcrb180 too high ! ");
psg_abort(1);
}
if(dpwr3 > 51)
{
printf("dpwr3 is too high; < 52\n");
psg_abort(1);
}
if(dpwr3_D > 49)
{
printf("dpwr3_D is too high; < 50\n");
psg_abort(1);
}
if(d1 < 1)
{
printf("d1 must be > 1\n");
psg_abort(1);
}
if(dpwrsed > 48)
{
printf("dpwrsed must be less than 49\n");
psg_abort(1);
}
if( gt0 > 5.0e-3 || gt1 > 5.0e-3 || gt2 > 5.0e-3 ||
gt3 > 5.0e-3 || gt4 > 5.0e-3 )
{ printf(" all values of gti must be < 5.0e-3\n");
psg_abort(1);
}
if(ix==1) {
printf("make sure that BigTC1 is set properly for your application\n");
printf("7 ms, neglecting relaxation \n");
}
/* Phase incrementation for hypercomplex 2D data */
if (phase == 2) {
tsadd(t1,1,4);
tsadd(t2,1,4);
tsadd(t3,1,4);
tsadd(t4,1,4);
}
if (phase2 == 2)
tsadd(t8,1,4);
/* Set up f1180 tau1 = t1 */
tau1 = d2;
tau1 = tau1 - 2.0*pw - 4.0/PI*pwC - POWER_DELAY - 2.0e-6 - PRG_START_DELAY
- PRG_STOP_DELAY - POWER_DELAY - 2.0e-6;
if(f1180[A] == 'y') {
tau1 += ( 1.0 / (2.0*sw1) );
if(tau1 < 0.4e-6) tau1 = 4.0e-7;
}
tau1 = tau1/2.0;
/* Set up f2180 tau2 = t2 */
tau2 = d3;
if(f2180[A] == 'y') {
tau2 += ( 1.0 / (2.0*sw2) );
if(tau2 < 0.4e-6) tau2 = 4.0e-7;
}
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2 ;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2) {
tsadd(t1,2,4);
tsadd(t9,2,4);
}
if( ix == 1) d3_init = d3 ;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2) {
tsadd(t8,2,4);
tsadd(t9,2,4);
}
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obspower(satpwr); /* Set transmitter power for 1H presaturation */
decpower(pwClvl); /* Set Dec1 power for hard 13C pulses */
dec2power(pwNlvl); /* Set Dec2 to low power */
/* Presaturation Period */
status(B);
if (fsat[0] == 'y')
{
obsoffset(tofps);
delay(2.0e-5);
rgpulse(d1,zero,2.0e-6,2.0e-6); /* presat with transmitter */
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
delay(2.0e-5);
if(fscuba[0] == 'y')
{
delay(2.2e-2);
rgpulse(pw,zero,2.0e-6,0.0);
rgpulse(2.0*pw,one,2.0e-6,0.0);
rgpulse(pw,zero,2.0e-6,0.0);
delay(2.2e-2);
}
}
else
{
delay(d1);
}
obspower(tpwr); /* Set transmitter power for hard 1H pulses */
obsoffset(tof);
txphase(t1);
decphase(zero);
dec2phase(zero);
delay(1.0e-5);
/* Begin Pulses */
status(C);
decoffset(dof_me);
lk_hold();
rcvroff();
delay(20.0e-6);
/* ensure that magnetization originates on 1H and not 13C */
if(dtt_flg[A] == 'y') {
obsoffset(tof_dtt);
obspower(tpwrs1);
shaped_pulse("H2Osinc",pwHs1,zero,10.0e-6,0.0);
obspower(tpwr);
obsoffset(tof);
}
decrgpulse(pwC,zero,0.0,0.0);
zgradpulse(gzlvl0,gt0);
delay(gstab);
rgpulse(pw,zero,0.0,0.0); /* 90 deg 1H pulse */
zgradpulse(gzlvl1,gt1);
delay(gstab);
delay(taua - gt1 -gstab);
simpulse(2.0*pw,2.0*pwC,zero,zero,0.0,0.0);
txphase(one);
delay(taua - gt1 - gstab);
zgradpulse(gzlvl1,gt1);
delay(gstab);
rgpulse(pw,one,0.0,0.0);
/* shaped_pulse */
obspower(tpwrs);
shaped_pulse("H2Osinc",pwHs,zero,2.0e-6,0.0);
obspower(tpwr);
/* shaped_pulse */
decoffset(dof); /* jump 13C to 40 ppm */
zgradpulse(gzlvl2,gt2);
delay(gstab);
decrgpulse(pwC,t1,4.0e-6,0.0); decphase(zero);
initval(1.0,v3);
decstepsize(decstep1);
dcplrphase(v3);
decpwrf(rfrb);
delay(BigTC - POWER_DELAY - WFG_START_DELAY);
decshaped_pulse(rb180.name,pwcrb180,zero,0.0,0.0);
dcplrphase(zero);
decphase(t2);
decpwrf(4095.0);
delay(BigTC - WFG_STOP_DELAY - POWER_DELAY);
decrgpulse(pwC,t2,0.0,0.0);
decphase(zero);
/* turn on 2H decoupling */
dec3phase(one);
dec3power(dpwr3);
dec3rgpulse(pwd1,one,4.0e-6,0.0);
dec3phase(zero);
dec3unblank();
dec3power(dpwr3_D);
dec3prgon(dseq3,pwd,dres3);
dec3on();
/* turn on 2H decoupling */
initval(1.0,v3);
decstepsize(decstep1);
dcplrphase(v3);
decpwrf(rfrb);
delay(BigTC1 - POWER_DELAY - 4.0e-6 - pwd1
- POWER_DELAY - PRG_START_DELAY - POWER_DELAY - WFG_START_DELAY);
decshaped_pulse(rb180.name,pwcrb180,zero,0.0,0.0);
dcplrphase(zero);
decphase(t3);
decpwrf(4095.0);
delay(BigTC1 - WFG_STOP_DELAY - POWER_DELAY);
decrgpulse(pwC,t3,0.0,0.0);
decpwrf(rfrb_cg); decphase(zero);
if(taub > pwrb_cg)
delay(taub/2.0 - pwrb_cg/2.0 - POWER_DELAY - WFG_START_DELAY);
decshaped_pulse(rb180_cg.name,pwrb_cg,zero,0.0,0.0);
decpwrf(rfrb);
if(taub > pwrb_cg)
delay(taub/2.0 - pwrb_cg/2.0 - WFG_STOP_DELAY - POWER_DELAY - SAPS_DELAY
- 2.0e-6 - WFG_START_DELAY);
initval(1.0,v3);
decstepsize(decstep1);
dcplrphase(v3);
decshaped_pulse(rb180.name,pwcrb180,zero,2.0e-6,0.0);
dcplrphase(zero);
decpwrf(rfrb_cg); decphase(zero);
if(taub > pwrb_cg)
delay(taub/2.0 - pwrb_cg/2.0 - WFG_STOP_DELAY - SAPS_DELAY
- POWER_DELAY - WFG_START_DELAY);
decshaped_pulse(rb180_cg.name,pwrb_cg,zero,0.0,0.0);
decpwrf(4095.0); decphase(t4);
if(taub > pwrb_cg)
delay(taub/2.0 - pwrb_cg/2.0 - WFG_STOP_DELAY - POWER_DELAY);
decrgpulse(pwC,t4,0.0,0.0);
if(C_flg[A] == 'n') {
decpower(dpwrsed); decunblank(); decphase(zero); delay(2.0e-6);
decprgon(cosed.name,pwsed,dressed);
decon();
delay(tau1);
rgpulse(2.0*pw,zero,0.0,0.0);
delay(tau1);
decoff();
decprgoff();
decblank();
decpower(pwClvl);
}
else
simpulse(2.0*pw,2.0*pwC,zero,zero,4.0e-6,4.0e-6);
decrgpulse(pwC,t5,2.0e-6,0.0);
decpwrf(rfrb_cg); decphase(zero);
if(taub > pwrb_cg)
delay(taub/2.0 - pwrb_cg/2.0 - POWER_DELAY - WFG_START_DELAY);
decshaped_pulse(rb180_cg.name,pwrb_cg,zero,0.0,0.0);
decpwrf(rfrb);
if(taub > pwrb_cg)
delay(taub/2.0 - pwrb_cg/2.0 - WFG_STOP_DELAY - POWER_DELAY - SAPS_DELAY
- 2.0e-6 - WFG_START_DELAY);
initval(1.0,v3);
decstepsize(decstep1);
dcplrphase(v3);
decshaped_pulse(rb180.name,pwcrb180,zero,2.0e-6,0.0);
dcplrphase(zero);
decpwrf(rfrb_cg); decphase(zero);
if(taub > pwrb_cg)
delay(taub/2.0 - pwrb_cg/2.0 - WFG_STOP_DELAY - SAPS_DELAY
- POWER_DELAY - WFG_START_DELAY);
decshaped_pulse(rb180_cg.name,pwrb_cg,zero,0.0,0.0);
decpwrf(4095.0); decphase(t6);
if(taub > pwrb_cg)
delay(taub/2.0 - pwrb_cg/2.0 - WFG_STOP_DELAY - POWER_DELAY);
decrgpulse(pwC,t6,0.0,0.0);
decphase(zero);
initval(1.0,v3);
decstepsize(decstep1);
dcplrphase(v3);
decpwrf(rfrb);
delay(BigTC1 - POWER_DELAY - WFG_START_DELAY);
decshaped_pulse(rb180.name,pwcrb180,zero,0.0,0.0);
dcplrphase(zero);
decphase(t7);
decpwrf(4095.0);
delay(BigTC1 - WFG_STOP_DELAY - POWER_DELAY
- PRG_STOP_DELAY - POWER_DELAY - 4.0e-6 - pwd1);
/* 2H decoupling off */
dec3off();
dec3prgoff();
dec3blank();
dec3power(dpwr3);
dec3rgpulse(pwd1,three,4.0e-6,0.0);
/* 2H decoupling off */
decrgpulse(pwC,t7,0.0,0.0);
decphase(zero);
delay(tau2);
rgpulse(2.0*pw,zero,0.0,0.0);
initval(1.0,v3);
decstepsize(decstep1);
dcplrphase(v3);
decpwrf(rfrb);
delay(BigTC - 2.0*pw - POWER_DELAY - WFG_START_DELAY);
decshaped_pulse(rb180.name,pwcrb180,zero,0.0,0.0);
dcplrphase(zero);
decphase(t8);
decpwrf(4095.0);
delay(BigTC - tau2 - WFG_STOP_DELAY - POWER_DELAY - 4.0e-6);
decrgpulse(pwC,t8,4.0e-6,0.0);
decoffset(dof_me);
zgradpulse(gzlvl3,gt3);
delay(gstab);
lk_sample();
/* shaped_pulse */
obspower(tpwrs);
shaped_pulse("H2Osinc",pwHs,two,2.0e-6,0.0);
obspower(tpwr);
/* shaped_pulse */
rgpulse(pw,zero,4.0e-6,0.0);
zgradpulse(gzlvl4,gt4);
delay(gstab);
delay(taua - gt4 -gstab
- POWER_DELAY - 2.0e-6 - WFG_START_DELAY
- pwHs - WFG_STOP_DELAY - POWER_DELAY - 2.0e-6);
/* shaped_pulse */
obspower(tpwrs);
shaped_pulse("H2Osinc",pwHs,two,2.0e-6,0.0);
obspower(tpwr);
/* shaped_pulse */
simpulse(2.0*pw,2.0*pwC,zero,zero,2.0e-6,0.0);
/* shaped_pulse */
obspower(tpwrs);
shaped_pulse("H2Osinc",pwHs,two,2.0e-6,0.0);
obspower(tpwr);
/* shaped_pulse */
zgradpulse(gzlvl4,gt4);
delay(gstab);
delay(taua - POWER_DELAY - WFG_START_DELAY
- pwHs - WFG_STOP_DELAY - POWER_DELAY
- gt4 - gstab - 2.0*POWER_DELAY);
decpower(dpwr); /* Set power for decoupling */
dec2power(dpwr2);
/* rcvron(); */ /* Turn on receiver to warm up before acq */
/* BEGIN ACQUISITION */
status(D);
setreceiver(t9);
}
void pulsesequence() {
//======================================================
// Define Variables and Objects and Get Parameter Values
//======================================================
// --------------------------------
// Acquisition Decoupling
// -------------------------------
char Xseq[MAXSTR];
getstr("Xseq",Xseq);
DSEQ dec = getdseq("X");
strncpy(dec.t.ch,"dec",3);
putCmd("chXtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chXspinal='dec'\n");
//-------------------------------------
// Homonuclear Decoupling During Echo
//-------------------------------------
MPDEC homo1 = getmpdec("hdec1H",0,0.0,0.0,0,1);
strncpy(homo1.mps.ch,"obs",3);
putCmd("chHhdec1='obs'\n");
// --------------------
// H echo calculation
// --------------------
double t1Hecho = getval("t1Hecho") - getval("pwHecho")/2.0 -
((!strcmp(homo1.dm,"y"))?getval("pwHshort1")*2.:0.0);
if (t1Hecho < 0.0) t1Hecho = 0.0;
double t2Hecho = getval("t2Hecho") - getval("pwHecho")/2.0 -
((!strcmp(homo1.dm,"y"))?getval("pwHshort1")*2.:0.0) -
getval("rd")- getval("ad");
if (t2Hecho < 0.0) t2Hecho = 0.0;
double t1H_echo = 0.0;
double t2H_echo = 0.0;
double t1H_left = 0.0;
double t2H_left = 0.0;
if (!strcmp(homo1.dm,"y")) {
t2H_echo = homo1.mps.t*((int)(t2Hecho/homo1.mps.t));
t2H_left = t2Hecho - t2H_echo;
t1H_echo = t2H_echo;
t1H_left = t1Hecho - t1H_echo;
}
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
//----------------------
// Dutycycle Protection
//----------------------
DUTY d = init_dutycycle();
d.dutyon = getval("pwH90");
d.dutyoff = d1 + 4.0e-6;
if (!strcmp(homo1.dm,"y"))
d.dutyon += t1H_echo + t2H_echo;
else
d.dutyoff += t1H_echo + t2H_echo;
d.c1 = d.c1 + (!strcmp(Xseq,"tppm"));
d.c1 = d.c1 + ((!strcmp(Xseq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(Xseq,"spinal"));
d.c2 = d.c2 + ((!strcmp(Xseq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
//------------------------
// Set Phase Tables
//-----------------------
settable(phH90,4,table1);
settable(phHecho,8,table2);
settable(phRec,4,table3);
setreceiver(phRec);
//=======================
// Begin Sequence
//=======================
txphase(phH90); decphase(zero);
obspwrf(getval("aH90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
//------------------------
// H Direct Polarization
//------------------------
rgpulse(getval("pwH90"),phH90,0.0,0.0);
obsunblank(); decunblank(); _unblank34();
// -----------------------------
// H Hahn Echo
// -----------------------------
if (!strcmp(homo1.dm,"y")) {
delay (t1H_left);
if (getval("pwHshort1") > 0.0 ) {
obspwrf(getval("aHhdec1"));
rgpulse(getval("pwHshort1"),three,0.0,0.0);
obsunblank();
}
if (!strcmp(homo1.dm,"y")) _mpseqon(homo1.mps,zero);
delay(t1H_echo);
if (!strcmp(homo1.dm,"y")) _mpseqoff(homo1.mps);
if (getval("pwHshort1") > 0.0 ) {
obspwrf(getval("aHhdec1")); txphase(one);
rgpulse(getval("pwHshort1"),one,0.0,0.0);
obsunblank();
}
}
else delay(t1Hecho);
txphase(phHecho);
obspwrf(getval("aHecho"));
rgpulse(getval("pwHecho"),phHecho,0.0,0.0);
obsunblank();
if (!strcmp(homo1.dm,"y")) {
if (getval("pwHshort1") > 0.0 ) {
obspwrf(getval("aHhdec1"));
rgpulse(getval("pwHshort1"),three,0.0,0.0);
obsunblank();
}
if (!strcmp(homo1.dm,"y")) _mpseqon(homo1.mps,zero);
delay(t2H_echo);
if (!strcmp(homo1.dm,"y")) _mpseqoff(homo1.mps);
if(getval("pwHshort1")>0 ) {
obspwrf(getval("aHhdec1"));
rgpulse(getval("pwHshort1"),one,0.0,0.0);
obsunblank();
}
delay(t2H_left);
}
else delay(t2Hecho);
//====================
// Begin Acquisition
//====================
_dseqon(dec);
obsblank(); decblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
CP hx = getcp("HX",0.0,0.0,0,1);
strncpy(hx.fr,"dec",3);
strncpy(hx.to,"obs",3);
putCmd("frHX='dec'\n");
putCmd("toHX='obs'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pw1Hhytrap") + getval("pw2Hhytrap") + getval("tHX");
d.dutyoff = d1 + 4.0e-6 + getval("t1HYtrap") + getval("t2HYtrap");
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(ph1Hhytrap,4,table1);
settable(phYhytrap,4,table2);
settable(ph2Hhytrap,4,table3);
settable(phXhx,4,table4);
settable(phHhx,4,table5);
settable(phRec,4,table6);
setreceiver(phRec);
// Begin Sequence
txphase(phXhx); decphase(ph1Hhytrap); dec2phase(phYhytrap);
obspwrf(getval("aXhx")); decpwrf(getval("aHhytrap")); dec2pwrf(getval("aYhytrap"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// TRAPDOR on H with Y Modulation
decrgpulse(getval("pw1Hhytrap"),ph1Hhytrap,0.0,0.0);
decphase(ph2Hhytrap);
decunblank();
dec2on();
delay(getval("t1HYtrap"));
dec2off();
decrgpulse(getval("pw2Hhytrap"),ph2Hhytrap,0.0,0.0);
decphase(phHhx);
decunblank();
decphase(phHhx);
decpwrf(getval("aHhx"));
delay(getval("t2HYtrap"));
// H to X Cross Polarization
_cp_(hx,phHhx,phXhx);
// Begin Acquisition
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
void pulsesequence() {
// Set the Maximum Dynamic Table Number
settablenumber(10);
setvvarnumber(30);
// Define Variables and Objects and Get Parameter Values
CP hx = getcp("HX",0.0,0.0,0,1);
strncpy(hx.fr,"dec",3);
strncpy(hx.to,"obs",3);
putCmd("frHX='dec'\n");
putCmd("toHX='obs'\n");
WMPA toss = gettoss5("tossX");
strncpy(toss.ch,"obs",3);
putCmd("chXtoss='obs'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwH90") + getval("tHX") + 5.0*toss.pw;
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = toss.rtau - 5.0*toss.pw + getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = toss.rtau - 5.0*toss.pw + getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phH90,22,table1);
settable(phfXhx,11,table2);
settable(phXhx,44,table3);
settable(phHhx,4,table4);
settable(phHdec,4,table5);
settable(phXtoss,44,table6);
settable(phRec,44,table7);
setreceiver(phRec);
obsstepsize(360.0/(PSD*8192));
// Begin Sequence
xmtrphase(phfXhx); txphase(phXhx); decphase(phH90);
obspwrf(getval("aXhx")); decpwrf(getval("aH90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H to X Cross Polarization - Shifted by -6COG11
decrgpulse(getval("pwH90"),phH90,0.0,0.0);
decphase(phHhx);
_cp_(hx,phHhx,phXhx);
decphase(phHdec);
// TOSS5 Sideband Suppression with included
// (-6,-5,-6,-5,-6)COG11 Cycle
_dseqon(dec);
_toss5(toss, phXtoss);
// Begin Acquisition with Quadrature Phase
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence()
{
/* DECLARE AND LOAD VARIABLES */
char f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
RELAY[MAXSTR], /* Insert HCCH-relay delay */
ribose[MAXSTR], /* ribose CHn groups only */
aromatic[MAXSTR], /* aromatic CHn groups only */
rna_stCshape[MAXSTR], /* calls sech/tanh pulses from shapelib */
rna_stCdec[MAXSTR], /* calls STUD+ waveforms from shapelib */
mag_flg[MAXSTR], /* Flag to use magic-angle gradients */
H2O_flg[MAXSTR],
sspul[MAXSTR],
SHAPE[MAXSTR],
STUD[MAXSTR]; /* apply automatically calculated STUD decoupling */
int t1_counter, /* used for states tppi in t1 */
t2_counter, /* used for states tppi in t2 */
ni2 = getval("ni2");
double tau1, /* t1 delay */
tau2, /* t2 delay */
delta1,delta2,
ni = getval("ni"),
lambda = 0.94/(4*getval("JCH")), /* 1/4J H1 evolution delay */
tCH = 1/(6.0*getval("JCH")), /* 1/4J C13 evolution delay */
tCC = 1/(8*getval("JCC")),
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rfC, /* maximum fine power when using pwC pulses */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
/* temporary Pbox parameters */
bw, pws, ofs, ppm, nst, /* bandwidth, pulsewidth, offset, ppm, # steps */
/* Sech/tanh inversion pulses automatically calculated by macro "rna_cal" */
/* and string parameter rna_stCshape calls them from your shapelib. */
rfst = 0.0, /* fine power for the rna_stCshape pulse, initialised */
dofa, /* dof shifted to 80 or 120ppm for ribose or aromatic spectra */
/* string parameter stCdec calls stud decoupling waveform from your shapelib.*/
studlvl, /* coarse power for STUD+ decoupling */
stdmf = getval("dmf80"), /* dmf for 80 ppm of STUD decoupling */
rf80 = getval("rf80"), /* rf in Hz for 80ppm STUD+ */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
sw1 = getval("sw1"),
sw2 = getval("sw2"),
gt3 = getval("gt3"),
gt4 = getval("gt4"),
gt5 = getval("gt5"),
gt7 = getval("gt7"),
gt8 = getval("gt8"),
gt9 = getval("gt9"),
gzcal = getval("gzcal"),
grecov = getval("grecov"),
gzlvl0 = getval("gzlvl0"),
gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5"),
gzlvl7 = getval("gzlvl7"), /* triax option */
gzlvl8 = getval("gzlvl8"),
gzlvl9 = getval("gzlvl9");
getstr("f1180",f1180);
getstr("mag_flg",mag_flg);
getstr("f2180",f2180);
getstr("RELAY",RELAY);
getstr("ribose",ribose);
getstr("aromatic",aromatic);
getstr("H2O_flg",H2O_flg);
getstr("sspul",sspul);
getstr("SHAPE",SHAPE);
getstr("STUD",STUD);
/* INITIALIZE VARIABLES */
/* maximum fine power for pwC pulses */
rfC = 4095.0;
setautocal(); /* activate auto-calibration flags */
if (autocal[0] == 'n')
{
/* 50ppm sech/tanh inversion */
rfst = (compC*4095.0*pwC*4000.0*sqrt((7.5*sfrq/600+3.85)/0.41));
rfst = (int) (rfst + 0.5);
}
else /* if autocal = 'y'(yes), 'q'(quiet), r(read), or 's'(semi) */
{
if(FIRST_FID) /* call Pbox */
{
ppm = getval("dfrq");
bw = 50.0*ppm; pws = 0.001; ofs = 0.0; nst = 500.0;
stC50 = pbox_makeA("rna_stC50", "sech", bw, pws, ofs, compC*pwC, pwClvl, nst);
if (dm3[B] == 'y') H2ofs = 3.2;
ofs_check(H1ofs, C13ofs, N15ofs, H2ofs);
}
rfst = stC50.pwrf;
}
strcpy(rna_stCshape, "rna_stC50");
strcpy(rna_stCdec, "wurst80");
studlvl = pwClvl + 20.0*log10(compC*pwC*4.0*rf80);
studlvl = (int) (studlvl + 0.5);
/* RIBOSE spectrum only, centered on 80ppm. */
if (ribose[A]=='y')
dofa = dof - 30.0*dfrq;
/* AROMATIC spectrum only, centered on 120ppm */
else
dofa = dof + 10*dfrq;
/* CHECK VALIDITY OF PARAMETER RANGES */
if( dpwrf < 4095 )
{ printf("reset dpwrf=4095 and recalibrate C13 90 degree pulse");
psg_abort(1); }
if( pwC > (24.0e-6*600.0/sfrq) )
{ printf("Increase pwClvl so that pwC < 24*600/sfrq");
psg_abort(1); }
if((dm[A] == 'y' || dm[B] == 'y' ))
{ printf("incorrect Dec1 decoupler flags! "); psg_abort(1);}
if((dm2[A] == 'y' || dm2[B] == 'y'))
{ printf("incorrect Dec2 decoupler flags! "); psg_abort(1);}
if((dm3[A] == 'y' || dm3[C] == 'y' ))
{printf("incorrect dec1 decoupler flags! Should be 'nyn' or 'nnn' ");
psg_abort(1); }
if ((dm3[B] == 'y' && dpwr3 > 44 ))
{ printf("Deuterium decoupling power too high ! "); psg_abort(1);}
if( dpwr > 50 )
{ printf("don't fry the probe, dpwr too large! "); psg_abort(1);}
if( dpwr2 > 50 )
{ printf("don't fry the probe, dpwr2 too large! "); psg_abort(1); }
/* CHOICE OF PULSE SEQUENCE */
if ( ((ribose[A]=='y') && (aromatic[A]=='y')) )
{ text_error("Choose ONE of ribose='y' OR aromatic='y' ! ");
psg_abort(1); }
if ( ((aromatic[A]=='y') && (RELAY[A]=='y')) )
{ text_error("No RELAY with aromatic='y' ! ");
psg_abort(1); }
/* LOAD VARIABLES */
settable(t1, 2, phi1);
settable(t2, 4, phi2);
settable(t3, 16, phi3);
settable(t4, 2, phi4);
settable(t11,8, rec);
/* INITIALIZE VARIABLES */
/* Phase incrementation for hypercomplex data */
if ( phase1 == 2 ) /* Hypercomplex in t1 */
tsadd(t1,1,4);
if ( phase2 == 2 )
tsadd(t2,1,4);
/* calculate modification to phases based on current t1 values
to achieve States-TPPI acquisition */
if(ix == 1) d2_init = d2;
t1_counter = (int)((d2-d2_init)*sw1 + 0.5);
if(t1_counter % 2)
{
tsadd(t1,2,4);
tsadd(t11,2,4);
}
/* calculate modification to phases based on current t2 values
to achieve States-TPPI acquisition */
if(ix == 1) d3_init = d3;
t2_counter = (int)((d3-d3_init)*sw2 + 0.5);
if(t2_counter % 2)
{
tsadd(t2,2,4);
tsadd(t11,2,4);
}
/* set up so that get (90, -180) phase corrects in F1 if f1180 flag is y */
tau1 = d2;
if(f1180[A] == 'y')
{
tau1 += (1.0/(2.0*sw1));
}
if (tau1 < 1.0e-6) tau1 = 0.0;
tau1 = tau1/2.0;
/* set up so that get (90, -180) phase corrects in F2 if f2180 flag is y */
tau2 = d3;
if(f2180[A] == 'y')
{
tau2 += (1.0/(2.0*sw2));
}
if (tau2 < 1.0e-6) tau2 = 0.0;
tau2 = tau2/2.0;
if (ni > 1)
delta1 = (double)(t1_counter*(lambda - gt5 - 0.2e-3))/((double)(ni-1));
else delta1 = 0.0;
if (ni2 > 1)
delta2 = (double)(t2_counter*(tCC - 0.6e-3))/((double)(ni2-1));
else delta2 = 0.0;
initval(7.0, v1);
obsstepsize (45.0);
/* BEGIN ACTUAL PULSE SEQUENCE */
status(A);
obsoffset(tof);
decoffset(dofa);
dec2offset(dof2);
obspower(tpwr-12);
decpower(pwClvl);
decpwrf(rfC);
dec2power(pwNlvl);
decphase(zero);
dec2phase(zero);
if (sspul[0] == 'y')
{
rgpulse(200*pw, one, 10.0e-6, 0.0e-6);
rgpulse(200*pw, zero, 0.0e-6, 1.0e-6);
}
obspower(tpwr);
xmtrphase(v1);
txphase(t1);
if (dm3[B] == 'y') lk_sample();
delay(d1);
if (dm3[B] == 'y') lk_hold();
rcvroff();
decrgpulse(pwC, zero, rof1, rof1);
delay(rof1);
zgradpulse(gzlvl0,0.5e-3);
delay(grecov);
if(dm3[B] == 'y') /*optional 2H decoupling on */
{
dec3unblank();
dec3rgpulse(1/dmf3, one, 0.0, 0.0);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
}
status(B);
rgpulse(pw, t1, 1.0e-4, rof1);
xmtrphase(zero);
txphase(zero);
zgradpulse(gzlvl5,gt5);
/*
decpwrf(rfst);
delay(lambda - gt5 - rof1 - SAPS_DELAY - GRADIENT_DELAY - POWER_DELAY -
WFG2_START_DELAY - 0.5e-3 + 70.0e-6 + tau1);
decshaped_pulse(rna_stCshape, 1.0e-3, zero, 0.0, 0.0);
delay(tau1 - delta1);
rgpulse(2.0*pw, zero, 0.0, rof1);
txphase(one);
decpwrf(rfC);
zgradpulse(gzlvl5,gt5);
delay(lambda - delta1 - gt5 - rof1 - GRADIENT_DELAY - POWER_DELAY - 0.5e-3 + 70.0e-6);
*/
delay(lambda - gt5 - rof1 - SAPS_DELAY - GRADIENT_DELAY + tau1);
decrgpulse(2*pwC, zero, 0.0, 0.0);
delay(tau1 - delta1);
rgpulse(2.0*pw, zero, 0.0, rof1);
txphase(one);
zgradpulse(gzlvl5,gt5);
delay(lambda - delta1 - gt5 - rof1 - GRADIENT_DELAY);
rgpulse(pw, one, 0.0, rof1);
decphase(t2);
txphase(zero);
if(mag_flg[A] == 'y')
magradpulse(gzcal*gzlvl3,gt3);
else
zgradpulse(gzlvl3,gt3);
delay(grecov);
decrgpulse(pwC, t2, rof1, 0.0);
decphase(zero);
delay(tau2);
dec2rgpulse(2.0*pwN,zero,0.0,0.0);
delay(tCH - 2*pwN);
rgpulse(2.0*pw, zero, 0.0, 0.0);
decphase(t3);
delay(tCC - tCH + tau2 - delta2 - 2.0*pw);
decrgpulse(2.0*pwC, t3, 0.0, 0.0);
decphase(t4);
delay(tCC - delta2);
decrgpulse(pwC, t4, 0.0, rof1);
txphase(zero);
decphase(zero);
if(RELAY[A] == 'y')
{
zgradpulse(gzlvl4, gt4);
delay(tCC - gt4 - GRADIENT_DELAY - pwC);
decrgpulse(2.0*pwC, zero, 0.0, 0.0);
zgradpulse(gzlvl4, gt4);
delay(tCC - gt4 - GRADIENT_DELAY - pwC);
decrgpulse(pwC, zero, 0.0, 0.0);
}
zgradpulse(gzlvl4,gt4);
delay(tCC - gt4);
decrgpulse(2.0*pwC, zero, 0.0, rof1);
if (H2O_flg[A] == 'y')
{
delay(tCC - gt4 - grecov - POWER_DELAY);
zgradpulse(gzlvl4,gt4);
txphase(one);
decphase(one);
delay(grecov);
decrgpulse(pwC, one, 0.0, rof1);
rgpulse(900*pw, one, 0.0, rof1);
txphase(zero);
rgpulse(500*pw, zero, rof1, rof1);
decphase(one);
if(mag_flg[A] == 'y')
magradpulse(gzcal*gzlvl7,gt7);
else
zgradpulse(gzlvl7,gt7);
delay(200.0e-6);
simpulse(pw, pwC, zero, one, 0.0, rof1);
decphase(zero);
zgradpulse(gzlvl4,gt4);
delay(tCH - gt4);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, rof1);
zgradpulse(gzlvl4,gt4);
delay(tCH - gt4);
}
else
{
delay(tCC - tCH - 2.0*pw - POWER_DELAY);
rgpulse(2.0*pw, zero, 0.0, rof1);
zgradpulse(gzlvl4,gt4);
delay(tCH - gt4 - rof1);
}
decrgpulse(pwC, zero, 0.0, rof1);
txphase(zero);
if(mag_flg[A] == 'y')
magradpulse(gzcal*gzlvl8,gt8);
else
zgradpulse(gzlvl8,gt8);
delay(grecov);
if(dm3[B] == 'y') /*optional 2H decoupling off */
{
dec3rgpulse(1/dmf3, three, 0.0, 0.0);
dec3blank();
setstatus(DEC3ch, FALSE, 'w', FALSE, dmf3);
dec3blank();
}
rgpulse(pw, zero, 0.0, rof1);
if (SHAPE[A] =='y')
{
decpwrf(rfst);
if(mag_flg[A] == 'y')
magradpulse(gzcal*gzlvl9,gt9);
else
zgradpulse(gzlvl9,gt9);
delay(lambda - gt9 - GRADIENT_DELAY - POWER_DELAY - WFG2_START_DELAY - 0.5e-3 + 70.0e-6);
simshaped_pulse("",rna_stCshape,2*pw, 1.0e-3, zero, zero, 0.0, rof1);
decphase(zero);
if(mag_flg[A] == 'y')
magradpulse(gzcal*gzlvl9,gt9);
else
zgradpulse(gzlvl9,gt9);
decpwrf(rfC);
if (STUD[A]=='y') decpower(studlvl); else decpower(dpwr);
delay(lambda - gt9 -rof1 -0.5*pw - 2*POWER_DELAY - GRADIENT_DELAY - 0.5e-3 + 70.0e-6);
}
else
{
if(mag_flg[A] == 'y')
magradpulse(gzcal*gzlvl9,gt9);
else
zgradpulse(gzlvl9,gt9);
delay(lambda - gt9 - GRADIENT_DELAY);
simpulse(2*pw, 2*pwC, zero, zero, 0.0, rof1);
if(mag_flg[A] == 'y')
magradpulse(gzcal*gzlvl9,gt9);
else
zgradpulse(gzlvl9,gt9);
if (STUD[A]=='y') decpower(studlvl); else decpower(dpwr);
delay(lambda - gt9 -rof1 -0.5*pw - POWER_DELAY - GRADIENT_DELAY);
}
rgpulse(pw, zero, rof1, rof2);
rcvron();
if (dm3[B] == 'y') lk_sample();
setreceiver(t11);
if ((STUD[A]=='y') && (dm[C] == 'y'))
{
decunblank();
decon();
decprgon(rna_stCdec,1/stdmf, 1.0);
startacq(alfa);
acquire(np, 1.0/sw);
decprgoff();
decoff();
decblank();
}
else
status(C);
setreceiver(t11);
}
void pulsesequence() {
// Define Variables and Objects and Get Parameter Values
MPSEQ fh = getfslg("fslgH",0,0.0,0.0,0,1);
strncpy(fh.ch,"dec",3);
putCmd("chHfslg='dec'\n");
double tHXhmqc = getval("tHXhmqc"); //parameters for hmqcHX implemented
double pwHhxhmqc = getval("pwHhxhmqc"); //directly in the pulse sequence
double pmHhxhmqc = getval("pmHhxhmqc");
double pwXhxhmqc = getval("pwXhxhmqc");
double aXhxhmqc = getval("aXhxhmqc");
double aHhxhmqc = getval("aHhxhmqc");
double d2init = getval("d2");
d2init = d2init - pwXhxhmqc;
if (d2init < 0.0) d2init = 0.0;
double d22 = d2init/2.0;
CP hx = getcp("HX",0.0,0.0,0,1);
strncpy(hx.fr,"dec",3);
strncpy(hx.to,"obs",3);
putCmd("frHX='dec'\n");
putCmd("toHX='obs'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
// Set Constant-time Period for d2.
if (d2_index == 0) d2_init = getval("d2");
double d2_ = (ni - 1)/sw1 + d2_init;
putCmd("d2acqret = %f\n",roundoff(d2_,12.5e-9));
putCmd("d2dwret = %f\n",roundoff(1.0/sw1,12.5e-9));
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwH90") + getval("tHX")+ 2.0*tHXhmqc + d2_ +
4.0*pmHhxhmqc + 2.0*pwHhxhmqc;
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phH90,4,table1);
settable(phXhx,4,table2);
settable(phHhx,4,table3);
settable(ph1Hhxhmqc,4,table4);
settable(ph2Hhxhmqc,4,table5);
settable(ph3Hhxhmqc,4,table6);
settable(ph4Hhxhmqc,4,table7);
settable(ph5Hhxhmqc,4,table8);
settable(phXhxhmqc,32,table9);
settable(ph6Hhxhmqc,4,table10);
settable(ph7Hhxhmqc,8,table11);
settable(ph8Hhxhmqc,4,table12);
settable(ph9Hhxhmqc,4,table13);
settable(phRec,16,table14);
// Add STATES TPPI (States with FAD)
tsadd(ph3Hhxhmqc,2*d2_index,4);
tsadd(ph4Hhxhmqc,2*d2_index,4);
tsadd(ph5Hhxhmqc,2*d2_index,4);
tsadd(ph6Hhxhmqc,2*d2_index,4);
tsadd(phRec,2*d2_index,4);
if (phase1 == 2) {
tsadd(ph3Hhxhmqc,1,4);
tsadd(ph4Hhxhmqc,1,4);
tsadd(ph5Hhxhmqc,1,4);
tsadd(ph6Hhxhmqc,1,4);
}
setreceiver(phRec);
// Begin Sequence
txphase(phXhx); decphase(phH90);
obspwrf(getval("aXhx")); decpwrf(getval("aH90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H to X Cross Polarization
decrgpulse(getval("pwH90"),phH90,0.0,0.0);
decphase(phHhx);
_cp_(hx,phHhx,phXhx);
// Begin hmqcHX with fh (FSLG) Between Pulses
obspwrf(aXhxhmqc);
_mpseqon(fh,ph1Hhxhmqc); // First "tau" period for J evolution
delay(tHXhmqc);
_mpseqoff(fh);
decpwrf(aHhxhmqc);
decrgpulse(pmHhxhmqc, ph2Hhxhmqc, 0.0, 0.0);// Create HX double-quantum coherence
decrgpulse(pwHhxhmqc, ph3Hhxhmqc, 0.0, 0.0);
decrgpulse(pmHhxhmqc, ph4Hhxhmqc, 0.0, 0.0);
_mpseqon(fh,ph5Hhxhmqc); // Begin F1 evolution with FSLG
delay(d22);
rgpulse(pwXhxhmqc, phXhxhmqc, 0.0,0.0);
delay(d22);
_mpseqoff(fh); // End F1 evolution with FSLG
decpwrf(aHhxhmqc);
decrgpulse(pmHhxhmqc, ph6Hhxhmqc, 0.0, 0.0);// Refocus HX double quantum coherence
decrgpulse(pwHhxhmqc, ph7Hhxhmqc, 0.0, 0.0);
decrgpulse(pmHhxhmqc, ph8Hhxhmqc, 0.0, 0.0);
_mpseqon(fh,ph9Hhxhmqc); // Second "tau" period for J evolution
delay(tHXhmqc);
_mpseqoff(fh);
// Begin Acquisition
decphase(phHhx);
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence() {
// Set the Maximum Dynamic Table and v-var Numbers
settablenumber(10);
setvvarnumber(30);
// Define Variables and Objects and Get Parameter Values
double aXprep = getval("aXprep");
double pwXprep = getval("pwXprep");
double phvXprep = getval("phXprep");
WMPA wsam = getwsamn("wsamX");
strncpy(wsam.ch,"obs",3);
putCmd("chXwsam='obs'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwXprep") + wsam.cycles1*wsam.cycles*wsam.pw;
d.dutyoff = d1 + 4.0e-6 + 5.0e-6 + wsam.r1 + wsam.r2 +
at - wsam.cycles1*wsam.cycles*wsam.pw;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phXprep,4,table1);
settable(phXwsam,4,table2);
settable(phRec,4,table3);
setreceiver(phRec);
// Set the Small-Angle Step
double obsstep = 360.0/(PSD*8192);
obsstepsize(obsstep);
int phfXprep = initphase(phvXprep,obsstep);
int phXzero = initphase(0.0,obsstep);
// Begin Sequence
xmtrphase(phfXprep); txphase(phXprep);
obspwrf(aXprep);
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// Standard 90-degree prepX pulse for SAM
startacq(5.0e-6);
rcvroff();
delay(wsam.r1);
rgpulse(pwXprep, phXprep, 0.0, 0.0);
xmtrphase(phXzero);
delay(wsam.r2);
// Apply WSAM Cycles
decblank(); _blank34();
_wsamn(wsam, phXwsam);
endacq();
obsunblank(); decunblank(); _unblank34();
}
void pulsesequence() {
// Define Variables and Objects and Get Parameter Values
CP hy = getcp("HY",0.0,0.0,0,1);
strncpy(hy.fr,"dec",3);
strncpy(hy.to,"dec2",4);
putCmd("frHY='dec'\n");
putCmd("toHY='dec2'\n");
CP yx = getcp("YX",0.0,0.0,0,1);
strncpy(yx.fr,"dec2",4);
strncpy(yx.to,"obs",3);
putCmd("frYX='dec2'\n");
putCmd("toYX='obs'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwY90") + getval("pwH90") + getval("tHY") + getval("tYX")
+ 2.0*getval("pwX90");
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Create Phasetables
settable(phH90,4,table1);
settable(phHhy,4,table2);
settable(phY90,4,table3);
settable(phYhy,4,table4);
settable(phHyx,4,table5);
settable(phYyx,4,table6);
settable(phXyx,8,table7);
settable(phRec,8,table8);
// Begin Sequence
setreceiver(phRec);
txphase(phXyx); decphase(phH90); dec2phase(phY90);
obspwrf(getval("aXyx")); decpwrf(getval("aH90")); dec2pwrf(getval("aY90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H to Y Cross Polarization with a Y Prepulse
dec2rgpulse(getval("pwY90"),phY90,0.0,0.0);
dec2phase(phYhy);
dec2pwrf(getval("aYyx"));
decrgpulse(getval("pwH90"),phH90,0.0,0.0);
decphase(phHhy);
_cp_(hy,phHhy,phYhy);
// Y to X Cross Polarization
decphase(phHyx); dec2phase(phYyx);
decpwrf(getval("aHyx"));
decunblank(); decon();
_cp_(yx,phYyx,phXyx);
decphase(phHhy);
dec2blank();
decoff();
// Begin Acquisition
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
CP hy = getcp("HY",0.0,0.0,0,1);
strncpy(hy.fr,"dec",3);
strncpy(hy.to,"dec2",4);
putCmd("frHY='dec'\n");
putCmd("toHY='dec2'\n");
CP yx = getcp("YX",0.0,0.0,0,1);
strncpy(yx.fr,"dec2",4);
strncpy(yx.to,"obs",3);
putCmd("frYX='dec2'\n");
putCmd("toYX='obs'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
// Set Constant-time Period for d2.
if (d2_index == 0) d2_init = getval("d2");
double d2_ = (ni - 1)/sw1 + d2_init;
putCmd("d2acqret = %f\n",roundoff(d2_,12.5e-9));
putCmd("d2dwret = %f\n",roundoff(1.0/sw1,12.5e-9));
// Set Constant-time Period for d3.
if (d3_index == 0) d3_init = getval("d3");
double d3_ = (ni - 1)/sw1 + d3_init;
putCmd("d3acqret = %f\n",roundoff(d3_,12.5e-9));
putCmd("d3dwret = %f\n",roundoff(1.0/sw2,12.5e-9));
// Set Mixing Period to N Rotor Cycles
double taur,mix,srate;
mix = getval("tXmix");
srate = getval("srate");
taur = 0.0;
if (srate >= 500.0)
taur = roundoff((1.0/srate), 0.125e-6);
else {
printf("ABORT: Spin Rate (srate) must be greater than 500\n");
psg_abort(1);
}
mix = roundoff(mix,taur);
mix = mix - getval("pwX90");
if (mix < 0.0) mix = 0.0;
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwY90") + getval("pwH90") + getval("tHY") + getval("tYX")
+ 2.0*getval("pwX90") + mix;
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = d2 + d3 + getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = d2 + d3 + getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Create Phasetables
settable(phH90,4,table1);
settable(phHhy,4,table2);
settable(phY90,4,table3);
settable(phYhy,4,table4);
settable(phHyx,4,table5);
settable(phYyx,4,table6);
settable(phXyx,8,table7);
settable(phXmix1,8,table8);
settable(phXmix2,8,table9);
settable(phRec,8,table10);
if (phase2 == 2)
tsadd(phXyx,1,4);
if (phase1 == 2)
tsadd(phYhy,1,4);
// Begin Sequence
setreceiver(phRec);
txphase(phXyx); decphase(phH90); dec2phase(phY90);
obspwrf(getval("aXyx")); decpwrf(getval("aH90")); dec2pwrf(getval("aY90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H to Y Cross Polarization with a Y Prepulse
dec2rgpulse(getval("pwY90"),phY90,0.0,0.0);
dec2phase(phYhy);
dec2pwrf(getval("aYyx"));
decrgpulse(getval("pwH90"),phH90,0.0,0.0);
decphase(phHhy);
_cp_(hy,phHhy,phYhy);
// F1 Indirect Period For Y
_dseqon(dec);
delay(d2);
_dseqoff(dec);
// Y to X Cross Polarization
decphase(phHyx); dec2phase(phYyx);
decpwrf(getval("aHyx"));
decunblank(); decon();
_cp_(yx,phYyx,phXyx);
decphase(phHhy);
decoff();
// F2 Indirect Period for X
txphase(phXmix1);
obspwrf(getval("aX90"));
_dseqon(dec);
delay(d3);
_dseqoff(dec);
// RAD(DARR) Mixing For X
decpwrf(getval("aHmix"));
decunblank(); decon();
rgpulse(getval("pwX90"),phXmix1,0.0,0.0);
txphase(phXmix2);
obsunblank();
delay(mix);
rgpulse(getval("pwX90"),phXmix2,0.0,0.0);
decoff();
// Begin Acquisition
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
void pulsesequence() {
// Set the Maximum Dynamic Table and v-var Numbers
settablenumber(20);
setvvarnumber(30);
// Define Variables and Objects and Get Parameter Values
MPSEQ dumbo = getdumbogen("dumboX","dcf1X",0,0.0,0.0,0,1);
strncpy(dumbo.ch,"obs",3);
putCmd("chXdumbo='obs'\n");
MPSEQ c7 = getpostc7("c7X",0,0.0,0.0,0,1);
MPSEQ c7ref = getpostc7("c7X",c7.iSuper,c7.phAccum,c7.phInt,1,1);
strncpy(c7.ch,"obs",3);
putCmd("chXc7='obs'\n");
WMPA wdumbo = getwdumbogen("wdumboX","dcfX");
strncpy(wdumbo.ch,"obs",3);
putCmd("chXwdumbo='obs'\n");
double tXzfinit = getval("tXzf"); //Define the Z-filter delay in the sequence
double tXzf = tXzfinit - 5.0e-6 - wdumbo.r1;
// Set Constant-time Period for d2.
if (d2_index == 0) d2_init = getval("d2");
double d2_ = (ni - 1)/sw1 + d2_init;
putCmd("d2acqret = %f\n",roundoff(d2_,12.5e-9));
putCmd("d2dwret = %f\n",roundoff(1.0/sw1,12.5e-9));
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = c7.t + getval("pwXtilt") + d2_ + getval("pwXtilt") + c7ref.t + getval("pwX90") +
+ wdumbo.q*wdumbo.cycles*wdumbo.pw;
d.dutyoff = 4.0e-6 + d1 + tXzfinit + wdumbo.r2 + at - wdumbo.q*wdumbo.cycles*wdumbo.pw;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(ph1Xc7,4,table1);
settable(phXdumbo,4,table2);
settable(ph2Xc7,4,table3);
settable(phX90,16,table4);
settable(phXwdumbo,4,table5);
settable(phRec,16,table6);
settable(ph1Xtilt,4,table7);
settable(ph2Xtilt,4,table8);
// Set the Small-Angle Prep Phase
double obsstep = 360.0/(PSD*8192);
obsstepsize(obsstep);
int phfX90 = initphase(0.0, obsstep);
//Add STATES Quadrature Phase
if (phase1 == 2)
initval((45.0/obsstep),v1);
else
initval(0.0,v1);
initval((d2*c7.of[0]*360.0/obsstep),v2);
initval(0.0,v3);
obsstepsize(obsstep);
setreceiver(phRec);
// Begin Sequence
xmtrphase(v1); txphase(ph1Xc7);
obspwrf(getval("aXc7"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// C7 Recoupling of 2Q coherence
_mpseq(c7, ph1Xc7);
// F1 Evolution With DUMBO
xmtrphase(v3);
if (!getval("scXdcf1")){
obspwrf(getval("aX90"));
rgpulse(getval("pwXtilt"),ph1Xtilt,0.0,0.0);
}
obspwrf(getval("aXdumbo"));
obsunblank();
_mpseqon(dumbo,phXdumbo);
delay(d2);
_mpseqoff(dumbo);
if (!getval("scXdcf1")){
obspwrf(getval("aX90"));
rgpulse(getval("pwXtilt"),ph2Xtilt,0.0,0.0);
}
obspwrf(getval("aX90"));
obsunblank();
// C7 Transfer to 1Q Coherence
xmtrphase(v2);
_mpseq(c7ref, ph2Xc7);
// Z-filter Delay
delay(tXzf);
// Detection Pulse
txphase(phX90);
obspwrf(getval("aX90"));
startacq(5.0e-6);
rcvroff();
delay(wdumbo.r1);
rgpulse(getval("pwX90"), phX90, 0.0, 0.0);
obsunblank();
xmtrphase(v3);
delay(wdumbo.r2);
// Apply WPMLG Cycles During Acqusition
decblank(); _blank34();
_wdumbo(wdumbo,phXwdumbo);
endacq();
obsunblank(); decunblank(); _unblank34();
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
double aYxy8 = getval("aYxy8");
double pwYxy8 = getval("pwYxy8");
double nYxy8 = getval("nYxy8");
int cycles = (int) nYxy8/2.0;
nYxy8 = 2.0*cycles;
int counter = (int) (nYxy8 - 1.0);
initval((nYxy8 - 1.0),v8);
double onYxy8 = getval("onYxy8");
double srate = getval("srate");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
DSEQ mix = getdseq("Hmix");
strncpy(mix.t.ch,"dec",3);
putCmd("chHmixtppm='mix'\n");
strncpy(mix.s.ch,"dec",3);
putCmd("chHmixspinal='mix'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwX90") + 4.0*nYxy8*pwYxy8 + getval("pwX180");
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d.c3 = d.c3 + (!strcmp(mix.seq,"tppm"));
d.c3 = d.c3 + ((!strcmp(mix.seq,"tppm")) && (mix.t.a > 0.0));
d.t3 = 2.0*nYxy8*(1.0/srate - 2.0*pwYxy8) + 1.0/srate - getval("pwX180");
d.c4 = d.c4 + (!strcmp(mix.seq,"spinal"));
d.c4 = d.c4 + ((!strcmp(mix.seq,"spinal")) && (mix.s.a > 0.0));
d.t4 = 2.0*nYxy8*(1.0/srate - 2.0*pwYxy8) + 1.0/srate - getval("pwX180");
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phX90,4,table1);
settable(ph1Yxy8,8,table2);
settable(ph2Yxy8,4,table3);
settable(phX180,4,table4);
settable(phRec,4,table5);
if (counter < 0) tsadd(phRec,2,4);
setreceiver(phRec);
// Begin Sequence
txphase(phX90); decphase(zero);
obspwrf(getval("aX90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// X Single Pulse
rgpulse(getval("pwX90"),phX90,0.0,0.0);
// xy8Y Period One
obspwrf(getval("aX180"));
txphase(phX180);
if (counter >= 0) {
_dseqon(mix);
delay(pwYxy8/2.0);
dec2pwrf(aYxy8);
sub(v1,v1,v1);
if (counter >= 1) {
if (counter > 1) loop(v8,v9);
getelem(ph1Yxy8,v1,v4);
incr(v1);
getelem(ph2Yxy8,ct,v2);
add(v4,v2,v2);
dec2phase(v2);
delay(0.5/srate - pwYxy8);
if (onYxy8 == 2)
dec2rgpulse(pwYxy8,v2,0.0,0.0);
else
delay(pwYxy8);
if (counter > 1) endloop(v9);
}
// X Refocussing Pulse
delay(0.5/srate - pwYxy8/2.0 - getval("pwX180")/2.0);
rgpulse(getval("pwX180"),phX180,0.0,0.0);
dec2pwrf(aYxy8);
delay(0.5/srate - pwYxy8/2.0 - getval("pwX180")/2.0);
// xy8Y Period Two
if (counter >= 1) {
if (counter > 1) loop(v8,v9);
if (onYxy8 == 2)
dec2rgpulse(pwYxy8,v2,0.0,0.0);
else
delay(pwYxy8);
getelem(ph1Yxy8,v1,v4);
incr(v1);
getelem(ph2Yxy8,ct,v2);
add(v4,v2,v2);
dec2phase(v2);
delay(0.5/srate - pwYxy8);
if (counter > 1) endloop(v9);
}
delay(pwYxy8/2.0);
_dseqoff(mix);
}
// Begin Acquisition
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
PBOXPULSE shp1 = getpboxpulse("sft1A",0,1);
PBOXPULSE shp2 = getpboxpulse("sft2A",0,1);
PBOXPULSE shp3 = getpboxpulse("sft3A",0,1);
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwX90") + shp1.pw + shp2.pw + shp3.pw;
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phX90,4,table1);
settable(phAsft1,4,table2);
settable(phAsft2,4,table3);
settable(phAsft3,4,table4);
settable(phRec,4,table5);
setreceiver(phRec);
// Begin Sequence
txphase(phX90); decphase(zero);
obspwrf(getval("aX90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// X Direct Polarization
rgpulse(getval("pwX90"),phX90,0.0,0.0);
delay(20.0e-6);
// X Shaped Pulse
_pboxpulse(shp1, phAsft1);
delay(20.0e-6);
// X Simultaneous Shaped Pulse
_pboxsimpulse(shp1,shp2,phAsft1,phAsft2);
delay(20.0e-6);
// X 3-channel Simultaneous Shaped Pulse
delay(20.0e-6);
_pboxsim3pulse(shp1,shp2,shp3,phAsft1,phAsft2,phAsft3);
// Begin Acquisition
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
void pulsesequence() {
//
// Set the Maximum Dynamic Table Number
//
settablenumber(10);
setvvarnumber(30);
//Define Variables and Objects and Get Parameter Values
CP hx = getcp("HX",0.0,0.0,0,1);
strncpy(hx.fr,"dec",3);
strncpy(hx.to,"obs",3);
putCmd("frHX='dec'\n");
putCmd("toHX='obs'\n");
WMPA cpmg = getcpmg("cpmgX");
strncpy(cpmg.ch,"obs",3);
putCmd("chXcpmg='obs'\n");
double aXecho = getval("aXecho"); // define the echoX group in the sequence
double t1Xechoinit = getval("t1Xecho");
double pwXecho = getval("pwXecho");
double t2Xechoinit = getval("t2Xecho");
double t1Xecho = t1Xechoinit - pwXecho/2.0 - getval("pwX90")/2.0;
if (t1Xecho < 0.0) t1Xecho = 0.0;
double t2Xecho = t2Xechoinit - pwXecho/2.0 - cpmg.r1 - cpmg.t2 - getval("ad");
if (t2Xecho < 0.0) t2Xecho = 0.0;
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwH90") + getval("tHX") + pwXecho + (cpmg.cycles - 1)*cpmg.pw;
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = t1Xecho + t2Xecho + getval("rd") + getval("ad") +
at - (cpmg.cycles - 1)*cpmg.pw;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = t1Xecho + t2Xecho + getval("rd") + getval("ad") +
at - (cpmg.cycles - 1)*cpmg.pw;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phH90,64,table1);
settable(phXhx,64,table2);
settable(phHhx,64,table3);
settable(phXecho,64,table4);
settable(phXcpmg,64,table5);
settable(phRec,64,table6);
setreceiver(phRec);
// Begin Sequence
txphase(phXhx); decphase(phH90);
obspwrf(getval("aXhx")); decpwrf(getval("aH90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H to X Cross Polarization
decrgpulse(getval("pwH90"),phH90,0.0,0.0);
decphase(phHhx);
_cp_(hx,phHhx,phXhx);
// H Decoupling On
decphase(zero);
_dseqon(dec);
// X Hahn Echo
txphase(phXecho);
obspwrf(aXecho);
delay(t1Xecho);
rgpulse(pwXecho,phXecho,0.0,0.0);
delay(t2Xecho);
// Apply CPMG Cycles
obsblank(); _blank34();
delay(cpmg.r1);
startacq(getval("ad"));
_cpmg(cpmg,phXcpmg);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
void pulsesequence() {
// Define Variables and Objects and Get Parameter Values
double aXhxpto2 = getval("aXhxpto2");
double pw1Xhxpto2 = getval("pw1Xhxpto2");
double pw2Xhxpto2 = getval("pw2Xhxpto2");
double t1HXpto2init = getval("t1HXpto2");
double tau1 = t1HXpto2init - pw1Xhxpto2/2.0;
double t2HXpto2init = getval("t2HXpto2");
double tau2 = t2HXpto2init - pw1Xhxpto2/2.0 - pw2Xhxpto2/2.0;
double t3HXpto2init = getval("t3HXpto2");
double tau3 = t3HXpto2init - pw2Xhxpto2/2.0;
MPSEQ r18 = getr1825("r18H",0,0.0,0.0,0,1);
MPSEQ r18ref = getr1825("r18H",r18.iSuper,r18.phAccum,r18.phInt,1,1);
strncpy(r18.ch,"dec",3);
strncpy(r18ref.ch,"dec",3);
putCmd("chHr18='dec'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = tau1 + tau2 + pw1Xhxpto2 + tau3 + pw2Xhxpto2;
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(ph1Hhxpto2,4,table1);
settable(ph2Hhxpto2,4,table2);
settable(ph1Xhxpto2,4,table3);
settable(ph2Xhxpto2,4,table4);
settable(phHdec,4,table5);
settable(phRec,4,table6);
setreceiver(phRec);
// Begin Sequence
txphase(ph1Xhxpto2); decphase(ph1Hhxpto2);
obspwrf(aXhxpto2); decpwrf(r18.a);
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H to X Cross Polarization with PRESTO1
_mpseqon(r18,ph1Hhxpto2);
delay(tau1);
rgpulse(pw1Xhxpto2/2.0,ph1Xhxpto2,0.0,0.0);
_mpseqoff(r18);
_mpseqon(r18ref,ph2Hhxpto2);
rgpulse(pw1Xhxpto2/2.0,ph1Xhxpto2,0.0,0.0);
delay(tau2);
_mpseqoff(r18ref);
decphase(zero);
_dseqon(dec);
rgpulse(pw2Xhxpto2,ph2Xhxpto2,0.0,0.0);
delay(tau3);
decphase(zero);
// Begin Acquisition
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
void pulsesequence()
{
char c1d[MAXSTR]; /* option to record only 1D C13 spectrum */
int ncyc;
double tau1 = 0.002, /* t1 delay */
post_del = 0.0,
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
compC = getval("compC"),
compH = getval("compH"),
mixpwr = getval("mixpwr"),
jCH = getval("jCH"),
gt0 = getval("gt0"),
gt1 = getval("gt1"),
gt2 = getval("gt2"),
gzlvl0 = getval("gzlvl0"),
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
grec = getval("grec"),
phase = getval("phase");
getstr("c1d",c1d);
ncyc=1;
if(jCH > 0.0)
tau1 = 0.25/jCH;
dbl(ct, v1); /* v1 = 0 */
mod4(v1,oph);
hlv(ct,v2);
add(v2,v1,v2);
if (phase > 1.5)
incr(v1); /* hypercomplex phase increment */
initval(2.0*(double)((int)(d2*getval("sw1")+0.5)%2),v10);
add(v1,v10,v1);
add(oph,v10,oph);
mod4(v1,v1); mod4(v2,v2); mod4(oph,oph);
assign(zero,v3);
if(FIRST_FID)
{
HHmix = pbox_mix("HHmix", "DIPSI2", mixpwr, pw*compH, tpwr);
if(c1d[A] == 'n')
{
opx("CHdec"); setwave("WURST2 30k/1.2m"); pbox_par("steps","600"); cpx(pwC*compC, pwClvl);
CHdec = getDsh("CHdec");
}
}
ncyc = (int) (at/HHmix.pw) + 1;
post_del = ncyc*HHmix.pw - at;
/* BEGIN PULSE SEQUENCE */
status(A);
zgradpulse(gzlvl0, gt0);
rgpulse(pw, zero, 0.0, 0.0); /* destroy H-1 magnetization*/
zgradpulse(gzlvl0, gt0);
delay(1.0e-4);
obspower(tpwr);
txphase(v1);
decphase(zero);
dec2phase(zero);
presat();
obspower(tpwr);
delay(1.0e-5);
status(B);
if(c1d[A] == 'y')
{
rgpulse(pw,v1,0.0,0.0); /* 1H pulse excitation */
delay(d2);
rgpulse(pw,two,0.0,0.0); /* 1H pulse excitation */
assign(oph,v3);
}
else
{
decunblank(); pbox_decon(&CHdec);
rgpulse(pw,v1,0.0,0.0); /* 1H pulse excitation */
txphase(zero);
delay(d2);
pbox_decoff(); decblank();
decpower(pwClvl); decpwrf(4095.0);
delay(tau1 - POWER_DELAY);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
txphase(one); decphase(one); dec2phase(one);
delay(tau1);
simpulse(pw, pwC, one, one, 0.0, 0.0);
txphase(zero); decphase(zero); dec2phase(zero);
delay(tau1);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
delay(tau1);
simpulse(0.0, pwC, zero, zero, 0.0, 0.0);
}
zgradpulse(gzlvl1, gt1);
delay(grec);
simpulse(0.0, pwC, zero, v3, 0.0, rof2);
txphase(v2);
obsunblank(); pbox_xmtron(&HHmix);
status(C);
setactivercvrs("ny");
startacq(alfa);
acquire(np,1.0/sw);
endacq();
delay(post_del);
pbox_xmtroff(); obsblank();
zgradpulse(gzlvl2, gt2);
obspower(tpwr);
delay(grec);
rgpulse(pw,zero,0.0,rof2); /* 1H pulse excitation */
status(D);
setactivercvrs("yn");
startacq(alfa);
acquire(np,1.0/sw);
endacq();
}
void pulsesequence() {
// Set the Maximum Dynamic Table Number
settablenumber(10);
setvvarnumber(30);
// Define Variables and Objects and Get Parameter Values
WMPA xx = getxx("xxX");
strncpy(xx.ch,"obs",3);
putCmd("chXxx='obs'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwXprep") + 2.0*xx.cycles*xx.pw;
d.dutyoff = d1 + 4.0e-6 + 5.0e-6 + xx.r1 + xx.r2 +
at - 2.0*xx.cycles*xx.pw;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phXprep,4,table1);
settable(phXxx,4,table2);
settable(phRec,4,table3);
setreceiver(phRec);
// Set the Small-Angle Prep Phase
double obsstep = 360.0/(PSD*8192);
obsstepsize(obsstep);
int phfXprep = initphase(getval("phXprep"), obsstep);
int phXzero = initphase(0.0, obsstep);
// Begin Sequence
xmtrphase(phfXprep); txphase(phXprep);
obspwrf(getval("aXprep"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// Preparation Pulse with Initial Point
startacq(5.0e-6);
rcvroff();
delay(xx.r1);
rgpulse(getval("pwXprep"), phXprep, 0.0, 0.0);
xmtrphase(phXzero);
// Apply Semi-windowless WHH4 Cycles
decblank(); _blank34();
_xx(xx, phXxx);
endacq();
obsunblank(); decunblank(); _unblank34();
}
pulsesequence()
{
/* DECLARE AND LOAD VARIABLES */
char f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
rna_stCdec[MAXSTR], /* calls STUD+ waveforms from shapelib */
STUD[MAXSTR]; /* apply automatically calculated STUD decoupling */
int icosel1, /* used to get n and p type */
icosel2,
t1_counter, /* used for states tppi in t1 */
t2_counter, /* used for states tppi in t2 */
ni2 = getval("ni2");
double tau1, /* t1 delay */
tau2, /* t2 delay */
del = getval("del"), /* time delays for CH coupling evolution */
del1 = getval("del1"),
del2 = getval("del2"),
/* STUD+ waveforms automatically calculated by macro "rnacal" */
/* and string parameter rna_stCdec calls them from your shapelib.*/
stdmf, /* dmf for STUD decoupling */
studlvl, /* coarse power for STUD+ decoupling */
rf80 = getval("rf80"), /* rf in Hz for 80ppm STUD+ */
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rfC, /* maximum fine power when using pwC pulses */
dofa, /* dof shifted to 80 ppm for ribose */
/* p_d is used to calculate the isotropic mixing on the Cab region */
p_d, /* 50 degree pulse for DIPSI-3 at rfd */
rfd, /* fine power for 35 ppm */
ncyc = getval("ncyc"), /* no. of cycles of DIPSI-3 */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
sw1 = getval("sw1"),
sw2 = getval("sw2"),
grecov = getval("grecov"), /* Gradient recovery delay, typically 150-200us */
gt1 = getval("gt1"), /* coherence pathway gradients */
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gt3 = getval("gt3"), /* other gradients */
gt5 = getval("gt5"),
gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5"),
gzlvl6 = getval("gzlvl6");
getstr("STUD",STUD);
getstr("f1180",f1180);
getstr("f2180",f2180);
/* LOAD PHASE TABLE */
settable(t3,2,phi3);
settable(t6,1,phi6);
settable(t5,4,phi5);
settable(t10,1,phi10);
settable(t11,4,rec);
/* INITIALIZE VARIABLES */
if( dpwrf < 4095 )
{ text_error("reset dpwrf=4095 and recalibrate C13 90 degree pulse");
psg_abort(1); }
/* maximum fine power for pwC pulses */
rfC = 4095.0;
/* Center dof in RIBOSE region on 80 ppm. */
dofa = dof - 30.0*dfrq;
/* dipsi-3 decoupling C-ribose */
p_d = (5.0)/(9.0*4.0*7000.0*(sfrq/800.0)); /* DIPSI-3 covers 35 ppm */
rfd = (compC*4095.0*pwC*5.0)/(p_d*9.0);
rfd = (int) (rfd + 0.5);
ncyc = (int) (ncyc + 0.5);
/* 80 ppm STUD+ decoupling */
strcpy(rna_stCdec, "wurst80");
stdmf = getval("dmf80");
studlvl = pwClvl + 20.0*log10(compC*pwC*4.0*rf80);
studlvl = (int) (studlvl + 0.5);
/* CHECK VALIDITY OF PARAMETER RANGES */
if( gt1 > 0.5*del - 0.5*grecov )
{ text_error(" gt1 is too big. Make gt1 less than %f.\n", (0.5*del - 0.5*grecov)); psg_abort(1);}
if((dm3[A] == 'y' || dm3[C] == 'y' ))
{ text_error("incorrect dec1 decoupler flags! Should be 'nyn' or 'nnn' "); psg_abort(1); }
if((dm2[A] == 'y' || dm2[B] == 'y'))
{ text_error("incorrect dec2 decoupler flags! Should be 'nnn' or 'nny' "); psg_abort(1); }
if((dm[A] == 'y' || dm[B] == 'y'))
{ text_error("incorrect dec1 decoupler flags! Should be 'nny' "); psg_abort(1); }
if( (((dm[C] == 'y') && (dm2[C] == 'y')) && (STUD[A] == 'y')) )
{ text_error("incorrect dec2 decoupler flags! Should be 'nnn' if STUD='y'"); psg_abort(1); }
if( dpwr > 50 )
{ text_error("don't fry the probe, DPWR too large! "); psg_abort(1); }
if( dpwr2 > 50 )
{ text_error("don't fry the probe, DPWR2 too large! "); psg_abort(1); }
if( (pw > 20.0e-6) && (tpwr > 56) )
{ text_error("don't fry the probe, pw too high ! "); psg_abort(1); }
if( (pwC > 40.0e-6) && (pwClvl > 56) )
{ text_error("don't fry the probe, pwN too high ! "); psg_abort(1); }
if( (pwN > 100.0e-6) && (pwNlvl > 56) )
{ text_error("don't fry the probe, pwN too high ! "); psg_abort(1); }
if ((dm3[B] == 'y' && dpwr3 > 44 ))
{ text_error ("Deuterium decoupling power too high ! "); psg_abort(1); }
if ((ncyc > 1 ) && (ix == 1))
{ text_error("mixing time is %f ms.\n",(ncyc*97.8*4*p_d)); }
/* PHASES AND INCREMENTED TIMES */
/* Phase incrementation for hypercomplex 2D data, States-Haberkorn element */
icosel1 = -1; icosel2 = -1;
if (phase1 == 2)
{ tsadd(t6,2,4); icosel1 = -1*icosel1; }
if (phase2 == 2)
{ tsadd(t10,2,4); icosel2 = -1*icosel2; tsadd(t6,2,4); }
/* Set up f1180 */
tau1 = d2;
if((f1180[A] == 'y') && (ni > 1.0))
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
tau1 = tau1/2.0;
/* Set up f2180 */
tau2 = d3;
if((f2180[A] == 'y') && (ni2 > 1.0))
{ tau2 += ( 1.0 / (2.0*sw2) ); if(tau2 < 0.2e-6) tau2 = 0.0; }
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{ tsadd(t3,2,4); tsadd(t11,2,4); }
if( ix == 1) d3_init = d3;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2)
{ tsadd(t5,2,4); tsadd(t11,2,4); }
/* BEGIN PULSE SEQUENCE */
status(A);
if (dm3[B]=='y') lk_sample();
delay(d1);
if (dm3[B]=='y') lk_hold();
rcvroff();
obspower(tpwr);
decpower(pwClvl);
dec2power(pwNlvl);
decpwrf(rfC);
obsoffset(tof);
decoffset(dofa);
dec2offset(dof2);
txphase(t3);
delay(1.0e-5);
decrgpulse(pwC, zero, 0.0, 0.0); /*destroy C13 magnetization*/
zgradpulse(gzlvl1, 0.5e-3);
delay(grecov/2);
decrgpulse(pwC, one, 0.0, 0.0);
zgradpulse(0.7*gzlvl1, 0.5e-3);
delay(5.0e-4);
if(dm3[B] == 'y') /*optional 2H decoupling on */
{
dec3unblank();
dec3rgpulse(1/dmf3, one, 0.0, 0.0);
dec3unblank();
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
}
rgpulse(pw, t3, 0.0, 0.0); /* 1H pulse excitation */
decphase(zero);
delay(0.5*del + tau1 - 2.0*pwC);
decrgpulse(2.0*pwC, zero, 0.0, 0.0);
txphase(zero);
delay(tau1);
rgpulse(2.0*pw, zero, 0.0, 0.0);
zgradpulse(icosel1*gzlvl1, 0.1*gt1);
decphase(t5);
delay(0.5*del - 0.1*gt1);
simpulse(pw, pwC, zero, t5, 0.0, 0.0);
zgradpulse(gzlvl3, gt3);
decphase(zero);
delay(0.5*del2 - gt3);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl3, gt3);
txphase(t6);
decphase(one);
delay(0.5*del2 - gt3);
simpulse(pw, pwC, t6, one, 0.0, 0.0);
zgradpulse(gzlvl4, gt3);
txphase(zero);
decphase(zero);
delay(0.5*del1 - gt3);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl4, gt3);
delay(0.5*del1 - gt3);
decrgpulse(pwC, zero, 0.0, 0.0);
decpwrf(rfd);
delay(2.0e-6);
initval(ncyc, v2);
starthardloop(v2);
decrgpulse(4.9*p_d,zero,0.0,0.0);
decrgpulse(7.9*p_d,two,0.0,0.0);
decrgpulse(5.0*p_d,zero,0.0,0.0);
decrgpulse(5.5*p_d,two,0.0,0.0);
decrgpulse(0.6*p_d,zero,0.0,0.0);
decrgpulse(4.6*p_d,two,0.0,0.0);
decrgpulse(7.2*p_d,zero,0.0,0.0);
decrgpulse(4.9*p_d,two,0.0,0.0);
decrgpulse(7.4*p_d,zero,0.0,0.0);
decrgpulse(6.8*p_d,two,0.0,0.0);
decrgpulse(7.0*p_d,zero,0.0,0.0);
decrgpulse(5.2*p_d,two,0.0,0.0);
decrgpulse(5.4*p_d,zero,0.0,0.0);
decrgpulse(0.6*p_d,two,0.0,0.0);
decrgpulse(4.5*p_d,zero,0.0,0.0);
decrgpulse(7.3*p_d,two,0.0,0.0);
decrgpulse(5.1*p_d,zero,0.0,0.0);
decrgpulse(7.9*p_d,two,0.0,0.0);
decrgpulse(4.9*p_d,two,0.0,0.0);
decrgpulse(7.9*p_d,zero,0.0,0.0);
decrgpulse(5.0*p_d,two,0.0,0.0);
decrgpulse(5.5*p_d,zero,0.0,0.0);
decrgpulse(0.6*p_d,two,0.0,0.0);
decrgpulse(4.6*p_d,zero,0.0,0.0);
decrgpulse(7.2*p_d,two,0.0,0.0);
decrgpulse(4.9*p_d,zero,0.0,0.0);
decrgpulse(7.4*p_d,two,0.0,0.0);
decrgpulse(6.8*p_d,zero,0.0,0.0);
decrgpulse(7.0*p_d,two,0.0,0.0);
decrgpulse(5.2*p_d,zero,0.0,0.0);
decrgpulse(5.4*p_d,two,0.0,0.0);
decrgpulse(0.6*p_d,zero,0.0,0.0);
decrgpulse(4.5*p_d,two,0.0,0.0);
decrgpulse(7.3*p_d,zero,0.0,0.0);
decrgpulse(5.1*p_d,two,0.0,0.0);
decrgpulse(7.9*p_d,zero,0.0,0.0);
decrgpulse(4.9*p_d,two,0.0,0.0);
decrgpulse(7.9*p_d,zero,0.0,0.0);
decrgpulse(5.0*p_d,two,0.0,0.0);
decrgpulse(5.5*p_d,zero,0.0,0.0);
decrgpulse(0.6*p_d,two,0.0,0.0);
decrgpulse(4.6*p_d,zero,0.0,0.0);
decrgpulse(7.2*p_d,two,0.0,0.0);
decrgpulse(4.9*p_d,zero,0.0,0.0);
decrgpulse(7.4*p_d,two,0.0,0.0);
decrgpulse(6.8*p_d,zero,0.0,0.0);
decrgpulse(7.0*p_d,two,0.0,0.0);
decrgpulse(5.2*p_d,zero,0.0,0.0);
decrgpulse(5.4*p_d,two,0.0,0.0);
decrgpulse(0.6*p_d,zero,0.0,0.0);
decrgpulse(4.5*p_d,two,0.0,0.0);
decrgpulse(7.3*p_d,zero,0.0,0.0);
decrgpulse(5.1*p_d,two,0.0,0.0);
decrgpulse(7.9*p_d,zero,0.0,0.0);
decrgpulse(4.9*p_d,zero,0.0,0.0);
decrgpulse(7.9*p_d,two,0.0,0.0);
decrgpulse(5.0*p_d,zero,0.0,0.0);
decrgpulse(5.5*p_d,two,0.0,0.0);
decrgpulse(0.6*p_d,zero,0.0,0.0);
decrgpulse(4.6*p_d,two,0.0,0.0);
decrgpulse(7.2*p_d,zero,0.0,0.0);
decrgpulse(4.9*p_d,two,0.0,0.0);
decrgpulse(7.4*p_d,zero,0.0,0.0);
decrgpulse(6.8*p_d,two,0.0,0.0);
decrgpulse(7.0*p_d,zero,0.0,0.0);
decrgpulse(5.2*p_d,two,0.0,0.0);
decrgpulse(5.4*p_d,zero,0.0,0.0);
decrgpulse(0.6*p_d,two,0.0,0.0);
decrgpulse(4.5*p_d,zero,0.0,0.0);
decrgpulse(7.3*p_d,two,0.0,0.0);
decrgpulse(5.1*p_d,zero,0.0,0.0);
decrgpulse(7.9*p_d,two,0.0,0.0);
endhardloop();
dec2phase(zero);
decphase(zero);
txphase(zero);
decpwrf(rfC);
delay(tau2);
sim3pulse(2.0*pw, 0.0, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
delay(tau2);
decpwrf(rfC);
zgradpulse(-icosel2*gzlvl2, 1.8*gt1);
delay(grecov+2.0e-6);
decrgpulse(2.0*pwC, zero, 0.0, 0.0);
decpwrf(rfC);
zgradpulse(icosel2*gzlvl2, 1.8*gt1);
delay(grecov + pwN);
decrgpulse(pwC, zero, 0.0, 0.0);
decpwrf(rfC);
decrgpulse(pwC, zero, 2.0e-6, 0.0);
zgradpulse(gzlvl5, gt5);
delay(0.5*del1 - gt5);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
txphase(one);
decphase(t10);
delay(0.5*del1 - gt5);
simpulse(pw, pwC, one, t10, 0.0, 0.0);
zgradpulse(gzlvl6, gt5);
txphase(zero);
decphase(zero);
delay(0.5*del2 - gt5);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl6, gt5);
delay(0.5*del2 - gt5);
simpulse(pw, pwC, zero, zero, 0.0, 0.0);
delay(0.5*del - 0.5*pwC);
simpulse(2.0*pw,2.0*pwC, zero, zero, 0.0, 0.0);
if (STUD[A]=='y') decpower(studlvl);
else
{
decpower(dpwr);
dec2power(dpwr2);
}
zgradpulse(gzlvl1, gt1); /* 2.0*GRADIENT_DELAY */
if(dm3[B] == 'y')
delay(0.5*del - gt1 -1/dmf3 - 2.0*GRADIENT_DELAY - POWER_DELAY);
else
delay(0.5*del - gt1 - 2.0*GRADIENT_DELAY - POWER_DELAY);
if(dm3[B] == 'y') /*optional 2H decoupling off */
{
dec3rgpulse(1/dmf3, three, 0.0, 0.0); dec3blank();
setstatus(DEC3ch, FALSE, 'w', FALSE, dmf3);
dec3blank();
}
decpower(dpwr); /* POWER_DELAY */
if (dm3[B]=='y') lk_sample();
if ((STUD[A]=='y') && (dm[C] == 'y'))
{decpower(studlvl);
decunblank();
decon();
decprgon(rna_stCdec,1/stdmf, 1.0);
startacq(alfa);
acquire(np, 1.0/sw);
decprgoff();
decoff();
decblank();
}
else
status(C);
setreceiver(t11);
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
CP hx = getcp("HX",0.0,0.0,0,1);
strncpy(hx.fr,"dec",3);
strncpy(hx.to,"obs",3);
putCmd("frHX='dec'\n");
putCmd("toHX='obs'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
DSEQ xdec = getdseq("X");
strncpy(xdec.t.ch,"obs",3); // These four statements assure
strncpy(xdec.s.ch,"obs",3); // that the X decoupling will
putCmd("chXtppm='obs'\n"); // be on X for either TPPM or
putCmd("chXspinal='obs'\n"); // SPINAL.
// Set Constant-time Period for d2.
if (d2_index == 0) d2_init = getval("d2");
double d2_ = (ni - 1)/sw1 + d2_init;
putCmd("d2acqret = %f\n",roundoff(d2_,12.5e-9));
putCmd("d2dwret = %f\n",roundoff(1.0/sw1,12.5e-9));
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = 3.0*getval("pwH90") + getval("pwHtilt") + getval("tHX");
d.dutyoff = d1 + 4.0e-6 + getval("tHmix");
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d.c3 = d.c3 + (!strcmp(xdec.seq,"tppm"));
d.c3 = d.c3 + ((!strcmp(xdec.seq,"tppm")) && (xdec.t.a > 0.0));
d.t3 = d2_;
d.c4 = d.c4 + (!strcmp(xdec.seq,"spinal"));
d.c4 = d.c4 + ((!strcmp(xdec.seq,"spinal")) && (xdec.s.a > 0.0));
d.t4 = d2_;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phH90,16,table1);
settable(phHmix1,16,table2);
settable(phHmix2,4,table3);
settable(phHtilt,4,table4);
settable(phXhx,4,table5);
settable(phHhx,4,table6);
settable(phRec,4,table7);
if (phase1 == 2) tsadd(phH90,1,4);
setreceiver(phRec);
// Begin Sequence
txphase(phXhx); decphase(phH90);
obspwrf(getval("aXhx")); decpwrf(getval("aH90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H Preparation and tilt
decrgpulse(getval("pwH90"),phH90,0.0,0.0);
decunblank();
// Delay for 1H Wideline T2
_dseqon(xdec);
delay(d2);
_dseqoff(xdec);
// Mix period for Spin Diffison
decrgpulse(getval("pwH90"),phHmix1,0.0,0.0);
delay(getval("tHmix"));
decrgpulse(getval("pwH90"),phHmix2,0.0,0.0);
// Tilt Pulse and Ramped H to X Cross Polarization with LG Offset
decrgpulse(getval("pwHtilt"),phHtilt,0.0,0.0);
decphase(phHhx);
_cp_(hx,phHhx,phXhx);
// Begin Acquisition
obsblank(); _blank34();
_dseqon(dec);
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence() {
// Set the Maximum Dynamic Table and v-var Numbers
settablenumber(10);
setvvarnumber(30);
// Define Variables and Objects and Get Parameter Values
double aXprep1 = getval("aXprep1"); // Define Tilted Pulses using "prep1X".
double pw1Xprep1 = getval("pw1Xprep1");
double pw2Xprep1 = getval("pw2Xprep1");
double phXprep1 = getval("phXprep1");
WMPA wpmlg = getwpmlg("wpmlgX");
strncpy(wpmlg.ch,"obs",3);
putCmd("chXwpmlg='obs'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pw1Xprep1") + getval("pw2Xprep1") + 2.0*wpmlg.q*wpmlg.cycles*wpmlg.pw;
d.dutyoff = d1 + 4.0e-6 + 5.0e-6 + wpmlg.r1 + wpmlg.r2 +
at - 2.0*wpmlg.q*wpmlg.cycles*wpmlg.pw;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(ph1Xprep1,4,table1);
settable(ph2Xprep1,4,table2);
settable(phXwpmlg,4,table3);
settable(phRec,4,table4);
setreceiver(phRec);
// Set the Small-Angle Step
double obsstep = 360.0/(PSD*8192);
obsstepsize(obsstep);
int phfXprep1 = initphase(phXprep1, obsstep);
int phXzero = initphase(0.0, obsstep);
// Begin Sequence
xmtrphase(phfXprep1); txphase(ph1Xprep1);
obspwrf(aXprep1);
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// Tilted Preparation Pulse for FSLG or PMLG "prep1X"
startacq(5.0e-6);
rcvroff();
delay(wpmlg.r1);
rgpulse(pw1Xprep1, ph1Xprep1, 0.0, 0.0);
rgpulse(pw2Xprep1, ph2Xprep1, 0.0, 0.0);
xmtrphase(phXzero);
delay(wpmlg.r2);
// Apply WPMLG Cycles
decblank(); _blank34();
_wpmlg(wpmlg, phXwpmlg);
endacq();
obsunblank(); decunblank(); _unblank34();
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
double aXecho = getval("aXecho");
double t1Xechoinit = getval("t1Xecho");
double pwXecho = getval("pwXecho");
double t2Xechoinit = getval("t2Xecho");
double t1Xecho = t1Xechoinit - pwXecho/2.0;
if (t1Xecho < 0.0) t1Xecho = 0.0;
double t2Xecho = t2Xechoinit - pwXecho/2.0 - getval("rd");
if (t2Xecho < 0.0) t2Xecho = 0.0;
CP hx = getcp("HX",0.0,0.0,0,1);
strncpy(hx.fr,"dec",3);
strncpy(hx.to,"obs",3);
putCmd("frHX='dec'\n");
putCmd("toHX='obs'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwH90") + getval("tHX") + pwXecho;
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phH90,4,table1);
settable(phXhx,4,table2);
settable(phHhx,4,table3);
settable(phXecho,16,table4);
settable(phRec,8,table5);
setreceiver(phRec);
// Begin Sequence
txphase(phXhx); decphase(phH90);
obspwrf(getval("aXhx")); decpwrf(getval("aH90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
// sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H to X Cross Polarization
decrgpulse(getval("pwH90"),phH90,0.0,0.0);
decphase(phHhx);
sp1on();
_cp_(hx,phHhx,phXhx);
sp1off();
// Begin Decoupling
_dseqon(dec);
// X Hahn Echo
txphase(phXecho);
obspwrf(aXecho);
delay(t1Xecho);
rgpulse(pwXecho,phXecho,0.0,0.0);
delay(t2Xecho);
// Begin Acquisition
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
void pulsesequence()
{
/* DECLARE AND LOAD VARIABLES */
char f1180[MAXSTR], /* Flag to start t1 @ halfdwell */
mag_flg[MAXSTR], /*magic angle gradient*/
f2180[MAXSTR], /* Flag to start t2 @ halfdwell */
stCdec[MAXSTR], /* calls STUD+ waveforms from shapelib */
STUD[MAXSTR]; /* apply automatically calculated STUD decoupling */
int icosel1, /* used to get n and p type */
icosel2,
t1_counter, /* used for states tppi in t1 */
t2_counter, /* used for states tppi in t2 */
ni2 = getval("ni2");
double tau1, /* t1 delay */
tau2, /* t2 delay */
del = getval("del"), /* time delays for CH coupling evolution */
BPdpwrspinlock, /* user-defined upper limit for spinlock(Hz) */
BPpwrlimits, /* =0 for no limit, =1 for limit */
del1 = getval("del1"),
del2 = getval("del2"),
/* STUD+ waveforms automatically calculated by macro "biocal" */
/* and string parameter stCdec calls them from your shapelib. */
stdmf, /* dmf for STUD decoupling */
studlvl, /* coarse power for STUD+ decoupling */
rf80 = getval("rf80"), /* rf in Hz for 80ppm STUD+ */
bw, ofs, ppm, /* temporary Pbox parameters */
pwClvl = getval("pwClvl"), /* coarse power for C13 pulse */
pwC = getval("pwC"), /* C13 90 degree pulse length at pwClvl */
rf0, /* maximum fine power when using pwC pulses */
/* p_d is used to calculate the isotropic mixing on the Cab region */
spinlock = getval("spinlock"), /* DIPSI-3 spinlock field strength in Hz */
p_d, /* 50 degree pulse for DIPSI-2 at rfd */
rfd, /* fine power for 7 kHz rf for 500MHz magnet */
ncyc = getval("ncyc"), /* no. of cycles of DIPSI-3 */
/* the following pulse lengths for SLP pulses are automatically calculated */
/* by the macro "ghcch_tocsy" . SLP pulse shapes, "offC10" etc are called */
/* directly from your shapelib. */
pwC10, /* 180 degree selective sinc pulse on CO(174ppm) */
pwZ, /* the largest of pwC10 and 2.0*pwN */
rf10, /* fine power for the pwC10 ("offC10") pulse */
compC = getval("compC"), /* adjustment for C13 amplifier compression */
pwNlvl = getval("pwNlvl"), /* power for N15 pulses */
pwN = getval("pwN"), /* N15 90 degree pulse length at pwNlvl */
sw1 = getval("sw1"),
sw2 = getval("sw2"),
gt1 = getval("gt1"), /* coherence pathway gradients */
gzcal = getval("gzcal"), /* G/cm to DAC coversion factor*/
gzlvl1 = getval("gzlvl1"),
gzlvl2 = getval("gzlvl2"),
gt3 = getval("gt3"), /* other gradients */
gt5 = getval("gt5"),
gzlvl3 = getval("gzlvl3"),
gzlvl4 = getval("gzlvl4"),
gzlvl5 = getval("gzlvl5"),
gzlvl6 = getval("gzlvl6");
getstr("STUD",STUD);
getstr("mag_flg",mag_flg);
getstr("f1180",f1180);
getstr("f2180",f2180);
strcpy(stCdec, "stCdec80");
stdmf = getval("dmf80");
studlvl = pwClvl + 20.0*log10(compC*pwC*4.0*rf80);
studlvl = (int) (studlvl + 0.5);
P_getreal(GLOBAL,"BPpwrlimits",&BPpwrlimits,1);
P_getreal(GLOBAL,"BPdpwrspinlock",&BPdpwrspinlock,1);
/* LOAD PHASE TABLE */
settable(t3,2,phi3);
settable(t6,1,phi6);
settable(t5,4,phi5);
settable(t10,1,phi10);
settable(t11,4,rec);
/* INITIALIZE VARIABLES */
if (BPpwrlimits > 0.5)
{
if (spinlock > BPdpwrspinlock)
{
spinlock = BPdpwrspinlock;
printf("spinlock too large, reset to user-defined limit (BPdpwrspinlock)");
psg_abort(1);
}
}
if( dpwrf < 4095 )
{ printf("reset dpwrf=4095 and recalibrate C13 90 degree pulse");
psg_abort(1); }
/* maximum fine power for pwC pulses */
rf0 = 4095.0;
setautocal(); /* activate auto-calibration flags */
if (autocal[0] == 'n')
{
/* "offC10": 180 degree one-lobe sinc pulse on CO, null at Ca 139ppm away */
pwC10 = getval("pwC10");
rf10 = (compC*4095.0*pwC*2.0*1.65)/pwC10; /* needs 1.65 times more */
rf10 = (int) (rf10 + 0.5); /* power than a square pulse */
if( pwC > (24.0e-6*600.0/sfrq) )
{ printf("Increase pwClvl so that pwC < 24*600/sfrq");
psg_abort(1);
}
}
else /* if autocal = 'y'(yes), 'q'(quiet), r(read), or 's'(semi) */
{
if(FIRST_FID) /* call Pbox */
{
ppm = getval("dfrq");
bw = 118.0*ppm; ofs = 139.0*ppm;
offC10 = pbox_make("offC10", "sinc180n", bw, ofs, compC*pwC, pwClvl);
if(dm3[B] == 'y') H2ofs = 3.2;
ofs_check(H1ofs, C13ofs, N15ofs, H2ofs);
}
rf10 = offC10.pwrf; pwC10 = offC10.pw;
}
/* dipsi-3 decoupling on CbCa */
p_d = (5.0)/(9.0*4.0*spinlock); /* DIPSI-3 Field Strength */
rfd = (compC*4095.0*pwC*5.0)/(p_d*9.0);
rfd = (int) (rfd + 0.5);
ncyc = (int) (ncyc + 0.5);
/* CHECK VALIDITY OF PARAMETER RANGES */
if( gt1 > 0.5*del - 1.0e-4)
{
printf(" gt1 is too big. Make gt1 less than %f.\n", (0.5*del - 1.0e-4));
psg_abort(1);
}
if( dm[A] == 'y' )
{
printf("incorrect dec1 decoupler flag! Should be 'nny' or 'nnn' ");
psg_abort(1);
}
if((dm2[A] == 'y' || dm2[C] == 'y'))
{
printf("incorrect dec2 decoupler flags! Should be 'nnn' ");
psg_abort(1);
}
if((dm3[A] == 'y' || dm3[C] == 'y'))
{
printf("incorrect dec3 decoupler flags! Should be 'nnn' or 'nyn' ");
psg_abort(1);
}
if( dpwr > 52 )
{
printf("don't fry the probe, DPWR too large! ");
psg_abort(1);
}
if( pw > 50.0e-6 )
{
printf("dont fry the probe, pw too high ! ");
psg_abort(1);
}
if( pwN > 100.0e-6 )
{
printf("dont fry the probe, pwN too high ! ");
psg_abort(1);
}
/* PHASES AND INCREMENTED TIMES */
/* Phase incrementation for hypercomplex 2D data, States-Haberkorn element */
icosel1 = -1; icosel2 = -1;
if (phase1 == 2)
{ tsadd(t6,2,4); icosel1 = -1*icosel1; }
if (phase2 == 2)
{ tsadd(t10,2,4); icosel2 = -1*icosel2; tsadd(t6,2,4); }
/* Set up f1180 */
tau1 = d2;
if((f1180[A] == 'y') && (ni > 1.0))
{ tau1 += ( 1.0 / (2.0*sw1) ); if(tau1 < 0.2e-6) tau1 = 0.0; }
tau1 = tau1/2.0;
/* Set up f2180 */
tau2 = d3;
if((f2180[A] == 'y') && (ni2 > 1.0))
{ tau2 += ( 1.0 / (2.0*sw2) ); if(tau2 < 0.2e-6) tau2 = 0.0; }
tau2 = tau2/2.0;
/* Calculate modifications to phases for States-TPPI acquisition */
if( ix == 1) d2_init = d2;
t1_counter = (int) ( (d2-d2_init)*sw1 + 0.5 );
if(t1_counter % 2)
{ tsadd(t3,2,4); tsadd(t11,2,4); }
if( ix == 1) d3_init = d3;
t2_counter = (int) ( (d3-d3_init)*sw2 + 0.5 );
if(t2_counter % 2)
{ tsadd(t5,2,4); tsadd(t11,2,4); }
/* BEGIN PULSE SEQUENCE */
status(A);
if ( dm3[B] == 'y' )
lk_sample();
if ((ni/sw1-d2)>0)
delay(ni/sw1-d2); /*decreases as t1 increases for const.heating*/
if ((ni2/sw2-d3)>0)
delay(ni2/sw2-d3); /*decreases as t2 increases for const.heating*/
delay(d1);
if ( dm3[B] == 'y' )
{ lk_hold(); lk_sampling_off();} /*freezes z0 correction, stops lock pulsing*/
rcvroff();
obspower(tpwr);
decpower(pwClvl);
dec2power(pwNlvl);
decpwrf(rf0);
obsoffset(tof);
txphase(t3);
delay(1.0e-5);
decrgpulse(pwC, zero, 0.0, 0.0); /*destroy C13 magnetization*/
zgradpulse(gzlvl1, 0.5e-3);
delay(1.0e-4);
decrgpulse(pwC, one, 0.0, 0.0);
zgradpulse(0.7*gzlvl1, 0.5e-3);
delay(5.0e-4);
if ( dm3[B] == 'y' ) /* begins optional 2H decoupling */
{
dec3rgpulse(1/dmf3,one,10.0e-6,2.0e-6);
dec3unblank();
dec3phase(zero);
delay(2.0e-6);
setstatus(DEC3ch, TRUE, 'w', FALSE, dmf3);
}
rgpulse(pw, t3, 0.0, 0.0); /* 1H pulse excitation */
decphase(zero);
delay(0.5*del + tau1 - 2.0*pwC);
decrgpulse(2.0*pwC, zero, 0.0, 0.0);
txphase(zero);
delay(tau1);
rgpulse(2.0*pw, zero, 0.0, 0.0);
if (mag_flg[A] == 'y')
{
magradpulse(icosel1*gzcal*gzlvl1,0.1*gt1);
}
else
{
zgradpulse(icosel1*gzlvl1, 0.1*gt1);
}
decphase(t5);
delay(0.5*del - 0.1*gt1);
simpulse(pw, pwC, zero, t5, 0.0, 0.0);
zgradpulse(gzlvl3, gt3);
decphase(zero);
delay(0.5*del2 - gt3);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl3, gt3);
txphase(t6);
decphase(one);
delay(0.5*del2 - gt3);
simpulse(pw, pwC, t6, one, 0.0, 0.0);
zgradpulse(gzlvl4, gt3);
txphase(zero);
decphase(zero);
delay(0.5*del1 - gt3);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl4, gt3);
delay(0.5*del1 - gt3);
decrgpulse(pwC, zero, 0.0, 0.0);
decpwrf(rfd);
delay(2.0e-6);
initval(ncyc, v2);
starthardloop(v2);
decrgpulse(4.9*p_d,zero,0.0,0.0);
decrgpulse(7.9*p_d,two,0.0,0.0);
decrgpulse(5.0*p_d,zero,0.0,0.0);
decrgpulse(5.5*p_d,two,0.0,0.0);
decrgpulse(0.6*p_d,zero,0.0,0.0);
decrgpulse(4.6*p_d,two,0.0,0.0);
decrgpulse(7.2*p_d,zero,0.0,0.0);
decrgpulse(4.9*p_d,two,0.0,0.0);
decrgpulse(7.4*p_d,zero,0.0,0.0);
decrgpulse(6.8*p_d,two,0.0,0.0);
decrgpulse(7.0*p_d,zero,0.0,0.0);
decrgpulse(5.2*p_d,two,0.0,0.0);
decrgpulse(5.4*p_d,zero,0.0,0.0);
decrgpulse(0.6*p_d,two,0.0,0.0);
decrgpulse(4.5*p_d,zero,0.0,0.0);
decrgpulse(7.3*p_d,two,0.0,0.0);
decrgpulse(5.1*p_d,zero,0.0,0.0);
decrgpulse(7.9*p_d,two,0.0,0.0);
decrgpulse(4.9*p_d,two,0.0,0.0);
decrgpulse(7.9*p_d,zero,0.0,0.0);
decrgpulse(5.0*p_d,two,0.0,0.0);
decrgpulse(5.5*p_d,zero,0.0,0.0);
decrgpulse(0.6*p_d,two,0.0,0.0);
decrgpulse(4.6*p_d,zero,0.0,0.0);
decrgpulse(7.2*p_d,two,0.0,0.0);
decrgpulse(4.9*p_d,zero,0.0,0.0);
decrgpulse(7.4*p_d,two,0.0,0.0);
decrgpulse(6.8*p_d,zero,0.0,0.0);
decrgpulse(7.0*p_d,two,0.0,0.0);
decrgpulse(5.2*p_d,zero,0.0,0.0);
decrgpulse(5.4*p_d,two,0.0,0.0);
decrgpulse(0.6*p_d,zero,0.0,0.0);
decrgpulse(4.5*p_d,two,0.0,0.0);
decrgpulse(7.3*p_d,zero,0.0,0.0);
decrgpulse(5.1*p_d,two,0.0,0.0);
decrgpulse(7.9*p_d,zero,0.0,0.0);
decrgpulse(4.9*p_d,two,0.0,0.0);
decrgpulse(7.9*p_d,zero,0.0,0.0);
decrgpulse(5.0*p_d,two,0.0,0.0);
decrgpulse(5.5*p_d,zero,0.0,0.0);
decrgpulse(0.6*p_d,two,0.0,0.0);
decrgpulse(4.6*p_d,zero,0.0,0.0);
decrgpulse(7.2*p_d,two,0.0,0.0);
decrgpulse(4.9*p_d,zero,0.0,0.0);
decrgpulse(7.4*p_d,two,0.0,0.0);
decrgpulse(6.8*p_d,zero,0.0,0.0);
decrgpulse(7.0*p_d,two,0.0,0.0);
decrgpulse(5.2*p_d,zero,0.0,0.0);
decrgpulse(5.4*p_d,two,0.0,0.0);
decrgpulse(0.6*p_d,zero,0.0,0.0);
decrgpulse(4.5*p_d,two,0.0,0.0);
decrgpulse(7.3*p_d,zero,0.0,0.0);
decrgpulse(5.1*p_d,two,0.0,0.0);
decrgpulse(7.9*p_d,zero,0.0,0.0);
decrgpulse(4.9*p_d,zero,0.0,0.0);
decrgpulse(7.9*p_d,two,0.0,0.0);
decrgpulse(5.0*p_d,zero,0.0,0.0);
decrgpulse(5.5*p_d,two,0.0,0.0);
decrgpulse(0.6*p_d,zero,0.0,0.0);
decrgpulse(4.6*p_d,two,0.0,0.0);
decrgpulse(7.2*p_d,zero,0.0,0.0);
decrgpulse(4.9*p_d,two,0.0,0.0);
decrgpulse(7.4*p_d,zero,0.0,0.0);
decrgpulse(6.8*p_d,two,0.0,0.0);
decrgpulse(7.0*p_d,zero,0.0,0.0);
decrgpulse(5.2*p_d,two,0.0,0.0);
decrgpulse(5.4*p_d,zero,0.0,0.0);
decrgpulse(0.6*p_d,two,0.0,0.0);
decrgpulse(4.5*p_d,zero,0.0,0.0);
decrgpulse(7.3*p_d,two,0.0,0.0);
decrgpulse(5.1*p_d,zero,0.0,0.0);
decrgpulse(7.9*p_d,two,0.0,0.0);
endhardloop();
dec2phase(zero);
decphase(zero);
txphase(zero);
decpwrf(rf10);
delay(tau2);
/* WFG3_START_DELAY */
sim3shaped_pulse("", "offC10", "", 2.0*pw, pwC10, 2.0*pwN, zero, zero, zero, 0.0, 0.0);
if(pwC10>2.0*pwN) pwZ=0.0; else pwZ=2.0*pwN - pwC10;
delay(tau2);
decpwrf(rf0);
if (mag_flg[A] == 'y')
{
magradpulse(-icosel2*gzcal*gzlvl2, 1.8*gt1);
}
else
{
zgradpulse(-icosel2*gzlvl2, 1.8*gt1);
}
delay(2.02e-4);
decrgpulse(2.0*pwC, zero, 0.0, 0.0);
decpwrf(rf10);
if (mag_flg[A] == 'y')
{
magradpulse(icosel2*gzcal*gzlvl2, 1.8*gt1);
}
else
{
zgradpulse(icosel2*gzlvl2, 1.8*gt1);
}
delay(2.0e-4 + WFG3_START_DELAY + pwZ);
decshaped_pulse("offC10", pwC10, zero, 0.0, 0.0);
decpwrf(rf0);
decrgpulse(pwC, zero, 2.0e-6, 0.0);
zgradpulse(gzlvl5, gt5);
delay(0.5*del1 - gt5);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl5, gt5);
txphase(one);
decphase(t10);
delay(0.5*del1 - gt5);
simpulse(pw, pwC, one, t10, 0.0, 0.0);
zgradpulse(gzlvl6, gt5);
txphase(zero);
decphase(zero);
delay(0.5*del2 - gt5);
simpulse(2.0*pw, 2.0*pwC, zero, zero, 0.0, 0.0);
zgradpulse(gzlvl6, gt5);
delay(0.5*del2 - gt5);
simpulse(pw, pwC, zero, zero, 0.0, 0.0);
delay(0.5*del - 0.5*pwC);
simpulse(2.0*pw,2.0*pwC, zero, zero, 0.0, 0.0);
if (mag_flg[A] == 'y')
magradpulse(gzcal*gzlvl1, gt1);
else
zgradpulse(gzlvl1, gt1);
rcvron();
if ((STUD[A]=='n') && (dm[C] == 'y'))
decpower(dpwr);
if ( dm3[B] == 'y' ) /* turns off 2H decoupling */
{
delay(0.5*del-40.0e-6 -gt1 -1/dmf3);
setstatus(DEC3ch, FALSE, 'c', FALSE, dmf3);
dec3rgpulse(1/dmf3,three,2.0e-6,2.0e-6);
dec3blank();
lk_autotrig(); /* resumes lock pulsing */
lk_sample();
if (mag_flg[A] == 'y')
statusdelay(C,40.0e-6 - 2.0*VAGRADIENT_DELAY - POWER_DELAY);
else
statusdelay(C,40.0e-6 - 2.0*GRADIENT_DELAY - POWER_DELAY);
}
else
{
delay(0.5*del-40.0e-6 -gt1);
if (mag_flg[A] == 'y')
statusdelay(C,40.0e-6 - 2.0*VAGRADIENT_DELAY - POWER_DELAY);
else
statusdelay(C,40.0e-6 - 2.0*GRADIENT_DELAY - POWER_DELAY);
}
if ((STUD[A]=='y') && (dm[C] == 'y'))
{decpower(studlvl);
decunblank();
decon();
decprgon(stCdec,1/stdmf, 1.0);
startacq(alfa);
acquire(np, 1.0/sw);
decprgoff();
decoff();
decblank();
}
setreceiver(t11);
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
SHAPE p1 = getpulse("90H",0.0,0.0,1,0);
strncpy(p1.pars.ch,"dec",3);
putCmd("chH90='dec'\n");
p1.pars.array = disarry("xx", p1.pars.array);
p1 = update_shape(p1,0.0,0.0,1);
MPSEQ ph = getpmlgxmx("pmlgH",0,0.0,0.0,1,0);
strncpy(ph.ch,"dec",3);
putCmd("chHpmlg='dec'\n");
double pwHpmlg = getval("pwHpmlg");
ph.nelem = (int) (d2/(2.0*pwHpmlg) + 0.1);
ph.array = disarry("xx", ph.array);
ph = update_mpseq(ph,0,p1.pars.phAccum,p1.pars.phInt,1);
SHAPE p2 = getpulse("90H",0.0,0.0,2,0);
strncpy(p2.pars.ch,"dec",3);
putCmd("chH90='dec'\n");
p2.pars.array = disarry("xx", p2.pars.array);
p2 = update_shape(p2,ph.phAccum,ph.phInt,2);
double pwX180 = getval("pwX180");
double d22 = ph.t/2.0 - pwX180/2.0;
if (d22 < 0.0) d22 = 0.0;
// CP hx and DSEQ dec Return to the Reference Phase
CP hx = getcp("HX",0.0,0.0,0,1);
strncpy(hx.fr,"dec",3);
strncpy(hx.to,"obs",3);
putCmd("frHX='dec'\n");
putCmd("toHX='obs'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
// Set Constant-time Period for d2.
if (d2_index == 0) d2_init = getval("d2");
double d2_ = (ni - 1)/sw1 + d2_init;
putCmd("d2acqret = %f\n",roundoff(d2_,12.5e-9));
putCmd("d2dwret = %f\n",roundoff(1.0/sw1,12.5e-9));
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = p1.pars.t + d2_ + p2.pars.t + getval("pwH90") + getval("pwHtilt") +
getval("tHX");
d.dutyoff = d1 + 4.0e-6 + getval("tHmix");
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(ph1H90,4,table1);
settable(phHpmlg,4,table2);
settable(ph2H90,4,table3);
settable(ph3H90,4,table4);
settable(phHtilt,4,table5);
settable(phXhx,4,table6);
settable(phHhx,4,table7);
settable(phRec,4,table8);
//Add STATES TPPI ("States with "FAD")
tsadd(phRec,2*d2_index,4);
if (phase1 == 2) {
tsadd(ph2H90,2*d2_index+3,4);
}
else {
tsadd(ph2H90,2*d2_index,4);
}
setreceiver(phRec);
// Begin Sequence
txphase(phXhx); decphase(ph1H90);
obspwrf(getval("aX180")); decpwrf(getval("aH90"));
obsunblank();decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// Offset H Preparation with a Tilt Pulse
_shape(p1,ph1H90);
// Offset SAMn Spinlock on H During F1 with Optional pwX180
_mpseqon(ph,phHpmlg);
delay(d22);
rgpulse(pwX180,zero,0.0,0.0);
obspwrf(getval("aX90"));
txphase(phXhx);
delay(d22);
_mpseqoff(ph);
// Offset 90-degree Pulse to Zed and Spin-Diffusion Mix
_shape(p2,ph2H90);
decpwrf(getval("aH90"));
delay(getval("tHmix"));
// H90, 35-degree Tilt and H-to-X Cross Polarization with LG Offset
decrgpulse(getval("pwH90"),ph3H90,0.0,0.0);
decunblank();
decrgpulse(getval("pwHtilt"),phHtilt,0.0,0.0);
decphase(phHhx);
_cp_(hx,phHhx,phXhx);
// Begin Acquisition
obsblank(); _blank34();
_dseqon(dec);
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
pulsesequence() {
// Define Variables and Objects and Get Parameter Values
CP hy = getcp("HY",0.0,0.0,0,1);
strncpy(hy.fr,"dec",3);
strncpy(hy.to,"dec2",4);
putCmd("frHY='dec'\n");
putCmd("toHY='dec2'\n");
GP inept = getinept("ineptYX");
strncpy(inept.ch1,"dec2",4);
strncpy(inept.ch2,"obs",3);
putCmd("ch1YXinept='dec2'\n");
putCmd("ch2YXinept='obs'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
DSEQ mix = getdseq("Hmix");
strncpy(mix.t.ch,"dec",3);
putCmd("chHmixtppm='dec'\n");
strncpy(mix.s.ch,"dec",3);
putCmd("chHmixspinal='dec'\n");
// Dutycycle Protection
double simpw1 = inept.pw1;
if (inept.pw2 > inept.pw1) simpw1 = inept.pw2;
double simpw2 = inept.pw3;
if (inept.pw4 > inept.pw3) simpw2 = inept.pw4;
DUTY d = init_dutycycle();
d.dutyon = getval("pwH90") + getval("tHY") + 2.0*simpw1 + 2.0*simpw2;
d.dutyoff = d1 + 4.0e-6;
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = inept.t1 + inept.t2 + inept.t3 + inept.t4 +
getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = inept.t1 + inept.t2 + inept.t3 + inept.t4 +
getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Set Phase Tables
settable(phH90,16,table1);
settable(phHhy,4,table2);
settable(phYhy,4,table3);
settable(ph1Yyxinept,4,table4);
settable(ph1Xyxinept,4,table5);
settable(ph2Yyxinept,4,table6);
settable(ph2Xyxinept,16,table7);
settable(ph3Yyxinept,8,table8);
settable(ph3Xyxinept,4,table9);
settable(phRec,8,table10);
setreceiver(phRec);
// Begin Sequence
txphase(ph1Xyxinept); decphase(phH90); dec2phase(phYhy);
obspwrf(getval("aXyxinept")); decpwrf(getval("aH90")); dec2pwrf(getval("aYhy"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H to Y Cross Polarization
decrgpulse(getval("pwH90"),phH90,0.0,0.0);
decphase(phHhy);
_cp_(hy,phHhy,phYhy);
decphase(zero);
// INEPT Transfer from Y to X
_dseqon(mix);
_ineptref(inept,ph1Yyxinept,ph1Xyxinept,ph2Yyxinept,ph2Xyxinept,ph3Yyxinept,ph3Xyxinept);
_dseqoff(mix);
// Begin Acquisition
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
void pulsesequence() {
// Define Variables and Objects and Get Parameter Values
CP hx = getcp("HX",0.0,0.0,0,1);
strncpy(hx.fr,"dec",3);
strncpy(hx.to,"obs",3);
putCmd("frHX='dec'\n");
putCmd("toHX='obs'\n");
MPSEQ spc5 = getspc5("spc5X",0,0.0,0.0,0,1);
MPSEQ spc5ref = getspc5("spc5X",spc5.iSuper,spc5.phAccum,spc5.phInt,1,1);
strncpy(spc5.ch,"obs",3);
putCmd("chXspc5='obs'\n");
DSEQ dec = getdseq("H");
strncpy(dec.t.ch,"dec",3);
putCmd("chHtppm='dec'\n");
strncpy(dec.s.ch,"dec",3);
putCmd("chHspinal='dec'\n");
// Set Constant-time Period for d2.
if (d2_index == 0) d2_init = getval("d2");
double d2_ = (ni - 1)/sw1 + d2_init;
putCmd("d2acqret = %f\n",roundoff(d2_,12.5e-9));
putCmd("d2dwret = %f\n",roundoff(1.0/sw1,12.5e-9));
//--------------------------------------
// Copy Current Parameters to Processed
//-------------------------------------
putCmd("groupcopy('current','processed','acquisition')");
// Dutycycle Protection
DUTY d = init_dutycycle();
d.dutyon = getval("pwH90") + getval("tHX") + getval("pwX90") +
spc5.t + spc5ref.t;
d.dutyoff = d1 + 4.0e-6 + 2.0*getval("tZF");
d.c1 = d.c1 + (!strcmp(dec.seq,"tppm"));
d.c1 = d.c1 + ((!strcmp(dec.seq,"tppm")) && (dec.t.a > 0.0));
d.t1 = d2_ + getval("rd") + getval("ad") + at;
d.c2 = d.c2 + (!strcmp(dec.seq,"spinal"));
d.c2 = d.c2 + ((!strcmp(dec.seq,"spinal")) && (dec.s.a > 0.0));
d.t2 = d2_ + getval("rd") + getval("ad") + at;
d = update_dutycycle(d);
abort_dutycycle(d,10.0);
// Create Phasetables
settable(phH90,4,table1);
settable(phHhx,4,table2);
settable(phXhx,4,table3);
settable(phXmix1,4,table4);
settable(phXmix2,4,table5);
settable(phRec,4,table6);
setreceiver(phRec);
if (phase1 == 2)
tsadd(phXhx,1,4);
// Begin Sequence
txphase(phXhx); decphase(phH90);
obspwrf(getval("aXhx")); decpwrf(getval("aH90"));
obsunblank(); decunblank(); _unblank34();
delay(d1);
sp1on(); delay(2.0e-6); sp1off(); delay(2.0e-6);
// H to X Cross Polarization
decrgpulse(getval("pwH90"),phH90,0.0,0.0);
decphase(phHhx);
_cp_(hx,phHhx,phXhx);
// F2 Indirect Period for X
obspwrf(getval("aX90"));
_dseqon(dec);
delay(d2);
_dseqoff(dec);
// Mixing with SPC5 Recoupling
rgpulse(getval("pwX90"),phXmix1,0.0,0.0);
obspwrf(getval("aXspc5"));
xmtrphase(v1); txphase(phXmix1);
delay(getval("tZF"));
decpwrf(getval("aHmix"));
decon();
_mpseq(spc5, phXmix1);
xmtrphase(v2); txphase(phXmix2);
_mpseq(spc5ref, phXmix2);
decoff();
obspwrf(getval("aX90"));
xmtrphase(zero); txphase(phXmix2);
delay(getval("tZF"));
rgpulse(getval("pwX90"),phXmix2,0.0,0.0);
// Begin Acquisition
_dseqon(dec);
obsblank(); _blank34();
delay(getval("rd"));
startacq(getval("ad"));
acquire(np, 1/sw);
endacq();
_dseqoff(dec);
obsunblank(); decunblank(); _unblank34();
}
