int main(int argc, char *argv[])
{
char *line;
char *sequence;
char *structure = NULL;
char fname[21];
char *ParamFile = NULL;
char *ns_bases = NULL, *c;
int i, length, l, sym, r;
int istty;
double deltaf, deltap=0;
int delta=100;
int n_back = 0;
int noconv = 0;
int circ=0;
int dos=0;
int zuker=0;
do_backtrack = 1;
dangles = 2;
for (i=1; i<argc; i++) {
if (argv[i][0]=='-')
switch ( argv[i][1] )
{
case 'T': if (argv[i][2]!='\0') usage();
if(i==argc-1) usage();
r=sscanf(argv[++i], "%lf", &temperature);
if (r!=1) usage();
break;
case 'p':
if (argv[i][2]!='\0') usage();
if(i==argc-1) usage();
(void) sscanf(argv[++i], "%d", &n_back);
init_rand();
break;
case 'n':
if ( strcmp(argv[i], "-noGU" )==0) noGU=1;
if ( strcmp(argv[i], "-noCloseGU" ) ==0) no_closingGU=1;
if ( strcmp(argv[i], "-noLP")==0) noLonelyPairs=1;
if ( strcmp(argv[i], "-nsp") ==0) {
if (i==argc-1) usage();
ns_bases = argv[++i];
}
if ( strcmp(argv[i], "-noconv")==0) noconv=1;
break;
case '4':
tetra_loop=0;
break;
case 'C':
fold_constrained=1;
break;
case 'D':
dos=1;
print_energy = -999999;
break;
case 'd': dangles=0;
if (argv[i][2]!='\0') {
r=sscanf(argv[i]+2, "%d", &dangles);
if (r!=1) usage();
}
break;
case 'P':
if (i==argc-1) usage();
ParamFile = argv[++i];
break;
case 's':
subopt_sorted=1;
break;
case 'l':
if (strcmp(argv[i],"-logML")==0) {
logML=1;
break;
}
else usage();
break;
case 'e':
if (i>=argc-1) usage();
if (strcmp(argv[i],"-ep")==0)
r=sscanf(argv[++i], "%lf", &deltap);
else {
r=sscanf(argv[++i], "%lf", &deltaf);
delta = (int) (0.1+deltaf*100);
}
if (r!=1) usage();
break;
case 'c':
if ( strcmp(argv[i], "-circ")==0) circ=1;
break;
case 'z':
zuker=1;
break;
default: usage();
}
}
if ((zuker)&&(circ)) {
printf("Sorry, zuker subopts not yet implemented for circfold\n");
usage();
}
if ((zuker)&&(n_back>0)) {
printf("Cna't do zuker subopts and stochastic subopts at the same time\n");
usage();
}
if (ParamFile != NULL)
read_parameter_file(ParamFile);
if (ns_bases != NULL) {
nonstandards = space(33);
c=ns_bases;
i=sym=0;
if (*c=='-') {
sym=1; c++;
}
while (*c) {
if (*c!=',') {
nonstandards[i++]=*c++;
nonstandards[i++]=*c;
if ((sym)&&(*c!=*(c-1))) {
nonstandards[i++]=*c;
nonstandards[i++]=*(c-1);
}
}
c++;
}
}
istty = isatty(fileno(stdout))&&isatty(fileno(stdin));
if ((fold_constrained)&&(istty)) {
printf("Input constraints using the following notation:\n");
/* printf("| : paired with another base\n"); */
printf(". : no constraint at all\n");
printf("x : base must not pair\n");
}
do { /* main loop: continue until end of file */
cut_point = -1;
if (istty) {
printf("\nInput string (upper or lower case); @ to quit\n");
if (!zuker)printf("Use '&' to connect 2 sequences that shall form a complex.\n");
printf("%s\n", scale);
}
fname[0]='\0';
if ((line = get_line(stdin))==NULL) break;
/* skip comment lines and get filenames */
while ((*line=='*')||(*line=='\0')||(*line=='>')) {
if (*line=='>')
(void) sscanf(line, ">%20s", fname);
free(line);
if ((line = get_line(stdin))==NULL) break;;
}
if ((line==NULL)||strcmp(line,"@")==0) break;
sequence = tokenize(line); /* frees line */
length = (int) strlen(sequence);
structure = (char *) space((unsigned) length+1);
if (fold_constrained) {
char *cstruc;
cstruc = tokenize(get_line(stdin));
if (cstruc!=NULL) {
strncpy(structure, cstruc, length);
for (i=0; i<length; i++)
if (structure[i]=='|')
nrerror("constraints of type '|' not allowed");
free(cstruc);
}
}
for (l = 0; l < length; l++) {
sequence[l] = toupper(sequence[l]);
if (!noconv && sequence[l] == 'T') sequence[l] = 'U';
}
if (istty) {
if (cut_point == -1)
printf("length = %d\n", length);
else
printf("length1 = %d\nlength2 = %d\n",
cut_point-1, length-cut_point+1);
}
if ((logML!=0 || dangles==1 || dangles==3) && dos==0)
if (deltap<=0) deltap=delta/100. +0.001;
if (deltap>0)
print_energy = deltap;
/* first lines of output (suitable for sort +1n) */
if (fname[0] != '\0')
printf("> %s [%d]\n", fname, delta);
if (n_back>0) { /* stochastic backtrack */
double mfe, kT;
char *ss;
st_back=1;
ss = (char *) space(strlen(sequence)+1);
strncpy(ss, structure, length);
mfe = fold(sequence, ss);
kT = (temperature+273.15)*1.98717/1000.; /* in Kcal */
pf_scale = exp(-(1.03*mfe)/kT/length);
strncpy(ss, structure, length);
/* ignore return value, we are not interested in the free energy */
(circ) ? (void) pf_circ_fold(sequence, ss) : (void) pf_fold(sequence, ss);
free(ss);
for (i=0; i<n_back; i++) {
char *s;
s =(circ) ? pbacktrack_circ(sequence) : pbacktrack(sequence);
printf("%s\n", s);
free(s);
}
free_pf_arrays();
} else if (!zuker) { /* normal subopt */
(circ) ? subopt_circ(sequence, structure, delta, stdout) : subopt(sequence, structure, delta, stdout);
if (dos) {
int i;
for (i=0; i<= MAXDOS && i<=delta/10; i++) {
printf("%4d %6d\n", i, density_of_states[i]);
}
}
} else { /* Zuker suboptimals */
SOLUTION *zr;
int i;
if (cut_point!=-1) {
printf("Sorry, zuker subopts not yet implemented for cofold\n");
usage();
}
zr = zukersubopt(sequence);
putoutzuker(zr);
(void)fflush(stdout);
for (i=0; zr[i].structure; i++) {
free(zr[i].structure);
}
free(zr);
}
(void)fflush(stdout);
free(sequence);
free(structure);
} while (1);
return 0;
}
int main(int argc, char *argv[]){
struct RNAsubopt_args_info args_info;
unsigned int input_type;
char fname[80], *cstruc, *sequence, *c, *input_string;
char *structure = NULL, *ParamFile = NULL, *ns_bases = NULL;
int i, length, l, sym, istty;
double deltaf, deltap;
int delta, n_back, noconv, circular, dos, zuker;
do_backtrack = 1;
dangles = 2;
delta = 100;
deltap = n_back = noconv = circular = dos = zuker = 0;
/*
#############################################
# check the command line parameters
#############################################
*/
if(RNAsubopt_cmdline_parser (argc, argv, &args_info) != 0) exit(1);
/* temperature */
if(args_info.temp_given) temperature = args_info.temp_arg;
/* structure constraint */
if(args_info.constraint_given) fold_constrained=1;
/* do not take special tetra loop energies into account */
if(args_info.noTetra_given) tetra_loop=0;
/* set dangle model */
if(args_info.dangles_given) dangles = args_info.dangles_arg;
/* do not allow weak pairs */
if(args_info.noLP_given) noLonelyPairs = 1;
/* do not allow wobble pairs (GU) */
if(args_info.noGU_given) noGU = 1;
/* do not allow weak closing pairs (AU,GU) */
if(args_info.noClosingGU_given) no_closingGU = 1;
/* do not convert DNA nucleotide "T" to appropriate RNA "U" */
if(args_info.noconv_given) noconv = 1;
/* take another energy parameter set */
if(args_info.paramFile_given) ParamFile = strdup(args_info.paramFile_arg);
/* Allow other pairs in addition to the usual AU,GC,and GU pairs */
if(args_info.nsp_given) ns_bases = strdup(args_info.nsp_arg);
/* energy range */
if(args_info.deltaEnergy_given) delta = (int) (0.1+args_info.deltaEnergy_arg*100);
/* energy range after post evaluation */
if(args_info.deltaEnergyPost_given) deltap = args_info.deltaEnergyPost_arg;
/* sorted output */
if(args_info.sorted_given) subopt_sorted = 1;
/* assume RNA sequence to be circular */
if(args_info.circ_given) circular=1;
/* stochastic backtracking */
if(args_info.stochBT_given){
n_back = args_info.stochBT_arg;
init_rand();
}
/* density of states */
if(args_info.dos_given){
dos = 1;
print_energy = -999999;
}
/* logarithmic multiloop energies */
if(args_info.logML_given) logML = 1;
/* zuker subopts */
if(args_info.zuker_given) zuker = 1;
if(zuker){
if(circular){
warn_user("Sorry, zuker subopts not yet implemented for circfold");
RNAsubopt_cmdline_parser_print_help();
exit(1);
}
else if(n_back>0){
warn_user("Can't do zuker subopts and stochastic subopts at the same time");
RNAsubopt_cmdline_parser_print_help();
exit(1);
}
}
/* free allocated memory of command line data structure */
RNAsubopt_cmdline_parser_free(&args_info);
/*
#############################################
# begin initializing
#############################################
*/
if (ParamFile != NULL) read_parameter_file(ParamFile);
if (ns_bases != NULL) {
nonstandards = space(33);
c=ns_bases;
i=sym=0;
if (*c=='-') {
sym=1; c++;
}
while (*c!='\0') {
if (*c!=',') {
nonstandards[i++]=*c++;
nonstandards[i++]=*c;
if ((sym)&&(*c!=*(c-1))) {
nonstandards[i++]=*c;
nonstandards[i++]=*(c-1);
}
}
c++;
}
}
istty = isatty(fileno(stdout))&&isatty(fileno(stdin));
if(fold_constrained && istty) print_tty_constraint(VRNA_CONSTRAINT_DOT | VRNA_CONSTRAINT_X);
/*
#############################################
# main loop: continue until end of file
#############################################
*/
do {
cut_point = -1;
/*
########################################################
# handle user input from 'stdin'
########################################################
*/
if(istty){
if (!zuker)
printf("Use '&' to connect 2 sequences that shall form a complex.\n");
print_tty_input_seq();
}
/* extract filename from fasta header if available */
fname[0] = '\0';
while((input_type = get_input_line(&input_string, 0)) == VRNA_INPUT_FASTA_HEADER){
printf(">%s\n", input_string);
(void) sscanf(input_string, "%42s", fname);
free(input_string);
}
/* break on any error, EOF or quit request */
if(input_type & (VRNA_INPUT_QUIT | VRNA_INPUT_ERROR)){ break;}
/* else assume a proper sequence of letters of a certain alphabet (RNA, DNA, etc.) */
else{
sequence = tokenize(input_string); /* frees input_string */
length = (int) strlen(sequence);
}
structure = (char *) space((unsigned) length+1);
if(noconv) str_RNA2RNA(sequence);
else str_DNA2RNA(sequence);
if(istty){
if (cut_point == -1)
printf("length = %d\n", length);
else
printf("length1 = %d\nlength2 = %d\n", cut_point-1, length-cut_point+1);
}
/* get structure constraint or break if necessary, entering an empty line results in a warning */
if (fold_constrained) {
input_type = get_input_line(&input_string, VRNA_INPUT_NOSKIP_COMMENTS);
if(input_type & VRNA_INPUT_QUIT){ break;}
else if((input_type & VRNA_INPUT_MISC) && (strlen(input_string) > 0)){
cstruc = tokenize(input_string);
strncpy(structure, cstruc, length);
for (i=0; i<length; i++)
if (structure[i]=='|')
nrerror("constraints of type '|' not allowed");
free(cstruc);
}
else warn_user("constraints missing");
}
/*
########################################################
# done with 'stdin' handling, now init everything properly
########################################################
*/
if((logML != 0 || dangles==1 || dangles==3) && dos == 0)
if(deltap<=0) deltap = delta/100. + 0.001;
if (deltap>0)
print_energy = deltap;
/* first lines of output (suitable for sort +1n) */
if (fname[0] != '\0')
printf("> %s [%d]\n", fname, delta);
/* stochastic backtracking */
if(n_back>0){
double mfe, kT;
char *ss;
st_back=1;
ss = (char *) space(strlen(sequence)+1);
strncpy(ss, structure, length);
mfe = fold(sequence, ss);
kT = (temperature+273.15)*1.98717/1000.; /* in Kcal */
pf_scale = exp(-(1.03*mfe)/kT/length);
strncpy(ss, structure, length);
/* ignore return value, we are not interested in the free energy */
(circular) ? (void) pf_circ_fold(sequence, ss) : (void) pf_fold(sequence, ss);
free(ss);
for (i=0; i<n_back; i++) {
char *s;
s =(circular) ? pbacktrack_circ(sequence) : pbacktrack(sequence);
printf("%s\n", s);
free(s);
}
free_pf_arrays();
}
/* normal subopt */
else if(!zuker){
(circular) ? subopt_circ(sequence, structure, delta, stdout) : subopt(sequence, structure, delta, stdout);
if (dos) {
int i;
for (i=0; i<= MAXDOS && i<=delta/10; i++) {
printf("%4d %6d\n", i, density_of_states[i]);
}
}
}
/* Zuker suboptimals */
else{
SOLUTION *zr;
int i;
if (cut_point!=-1) {
nrerror("Sorry, zuker subopts not yet implemented for cofold\n");
}
zr = zukersubopt(sequence);
putoutzuker(zr);
(void)fflush(stdout);
for (i=0; zr[i].structure; i++) {
free(zr[i].structure);
}
free(zr);
}
(void)fflush(stdout);
free(sequence);
free(structure);
} while (1);
return 0;
}
