void copyFromTopAlignment(AlignmentConstPtr topAlignment,
AlignmentPtr mainAlignment, const string &genomeName)
{
Genome *mainReplacedGenome = mainAlignment->openGenome(genomeName);
const Genome *topReplacedGenome = topAlignment->openGenome(genomeName);
topReplacedGenome->copyTopDimensions(mainReplacedGenome);
topReplacedGenome->copyTopSegments(mainReplacedGenome);
mainReplacedGenome->fixParseInfo();
// Copy bot segments for the parent and top segments for the
// siblings of the genome that's being replaced
Genome *mainParent = mainReplacedGenome->getParent();
const Genome *topParent = topReplacedGenome->getParent();
topParent->copyBottomDimensions(mainParent);
topParent->copyBottomSegments(mainParent);
mainParent->fixParseInfo();
vector<string> siblings = mainAlignment->getChildNames(mainParent->getName());
for (size_t i = 0; i < siblings.size(); i++)
{
if (siblings[i] != genomeName)
{
Genome *mainChild = mainAlignment->openGenome(siblings[i]);
const Genome *topChild = topAlignment->openGenome(siblings[i]);
topChild->copyTopDimensions(mainChild);
topChild->copyTopSegments(mainChild);
mainChild->fixParseInfo();
}
}
}
void GappedSegmentIteratorIndelTest::createCallBack(AlignmentPtr alignment)
{
addIdenticalParentChild(alignment, 1, 20, 5);
Genome* parent = alignment->openGenome(alignment->getRootName());
Genome* child = parent->getChild(0);
TopSegmentIteratorPtr ti = child->getTopSegmentIterator();
BottomSegmentIteratorPtr bi = parent->getBottomSegmentIterator();
// int i = 0;
// bool reversed = true;
bi = parent->getBottomSegmentIterator(0);
makeDelGap(bi);
bi = parent->getBottomSegmentIterator(3);
makeDelGap(bi);
/*
ti = child->getTopSegmentIterator(1);
makeInsGap(ti);
ti = child->getTopSegmentIterator(21);
makeInsGap(ti);
ti = child->getTopSegmentIterator(28);
makeInsGap(ti);
*/
/* for (size_t i = 0; i < 20; ++i)
{
cout << i << ": ";
bi = parent->getBottomSegmentIterator(i);
ti = child->getTopSegmentIterator(i);
cout << "ci=" << bi->getBottomSegment()->getChildIndex(0)
<< " pi=" << ti->getTopSegment()->getParentIndex() << endl;
}*/
}
void GappedSegmentSimpleIteratorTest2::createCallBack(AlignmentPtr alignment)
{
addIdenticalParentChild(alignment, 2, 100, 5);
Genome* parent = alignment->openGenome(alignment->getRootName());
Genome* child = parent->getChild(0);
TopSegmentIteratorPtr ti = child->getTopSegmentIterator();
BottomSegmentIteratorPtr bi = parent->getBottomSegmentIterator();
hal_index_t i = 0;
bool reversed = true;
while (ti != child->getTopSegmentEndIterator())
{
if (i % 5 == 0)
{
reversed = !reversed;
if (reversed && i < (hal_index_t)(parent->getNumBottomSegments() - 1))
{
makeInversion(ti, 5);
}
}
ti->toRight();
bi->toRight();
++i;
}
}
void copyFromBottomAlignment(AlignmentConstPtr bottomAlignment,
AlignmentPtr mainAlignment,
const string &genomeName)
{
// Copy genome & bottom segments for the genome that's being replaced
Genome *mainReplacedGenome = mainAlignment->openGenome(genomeName);
const Genome *botReplacedGenome = bottomAlignment->openGenome(genomeName);
botReplacedGenome->copyDimensions(mainReplacedGenome);
botReplacedGenome->copySequence(mainReplacedGenome);
botReplacedGenome->copyBottomDimensions(mainReplacedGenome);
botReplacedGenome->copyBottomSegments(mainReplacedGenome);
mainReplacedGenome->fixParseInfo();
// Copy top segments for the children
vector<string> children = mainAlignment->getChildNames(genomeName);
for (size_t i = 0; i < children.size(); i++)
{
Genome *mainChild = mainAlignment->openGenome(children[i]);
const Genome *botChild = bottomAlignment->openGenome(children[i]);
botChild->copyTopDimensions(mainChild);
botChild->copyTopSegments(mainChild);
mainChild->fixParseInfo();
}
}
void GenomeUpdateTest::createCallBack(AlignmentPtr alignment)
{
hal_size_t alignmentSize = alignment->getNumGenomes();
CuAssertTrue(_testCase, alignmentSize == 0);
Genome* ancGenome = alignment->addRootGenome("AncGenome", 0);
vector<Sequence::Info> seqVec(1);
seqVec[0] = Sequence::Info("Sequence", 1000000, 5000, 700000);
ancGenome->setDimensions(seqVec);
alignment->close();
alignment->open(_createPath, false);
ancGenome = alignment->openGenome("AncGenome");
seqVec[0] = Sequence::Info("Sequence", 10000005, 14000, 2000001);
ancGenome->setDimensions(seqVec);
}
void GappedSegmentSimpleIteratorTest::createCallBack(AlignmentPtr alignment)
{
addIdenticalParentChild(alignment, 2, 100, 5);
Genome* parent = alignment->openGenome(alignment->getRootName());
Genome* child = parent->getChild(0);
TopSegmentIteratorPtr ti = child->getTopSegmentIterator();
BottomSegmentIteratorPtr bi = parent->getBottomSegmentIterator();
int i = 0;
while (ti != child->getTopSegmentEndIterator())
{
if (i++ % 2)
{
ti->getTopSegment()->setParentReversed(true);
bi->getBottomSegment()->setChildReversed(0, true);
}
ti->toRight();
bi->toRight();
}
}
int main(int argc, char *argv[])
{
CLParserPtr optParser = initParser();
string inPath, botAlignmentPath, topAlignmentPath, parentName, insertName,
childName, leafName;
double upperBranchLength, leafBranchLength;
bool noMarkAncestors;
try {
optParser->parseOptions(argc, argv);
inPath = optParser->getArgument<string>("inFile");
botAlignmentPath = optParser->getArgument<string>("botAlignmentFile");
topAlignmentPath = optParser->getArgument<string>("topAlignmentFile");
parentName = optParser->getArgument<string>("parentName");
insertName = optParser->getArgument<string>("insertName");
childName = optParser->getArgument<string>("childName");
leafName = optParser->getArgument<string>("leafName");
upperBranchLength = optParser->getArgument<double>("upperBranchLength");
leafBranchLength = optParser->getArgument<double>("leafBranchLength");
noMarkAncestors = optParser->getFlag("noMarkAncestors");
} catch (exception &e) {
optParser->printUsage(cerr);
return 1;
}
AlignmentPtr mainAlignment = openHalAlignment(inPath, optParser);
AlignmentConstPtr botAlignment = openHalAlignment(botAlignmentPath,
optParser);
AlignmentConstPtr topAlignment = openHalAlignment(topAlignmentPath,
optParser);
mainAlignment->insertGenome(insertName, parentName, childName,
upperBranchLength);
mainAlignment->addLeafGenome(leafName, insertName, leafBranchLength);
// Insert the new intermediate node.
Genome *insertGenome = mainAlignment->openGenome(insertName);
const Genome *topInsertGenome = topAlignment->openGenome(insertName);
const Genome *botInsertGenome = botAlignment->openGenome(insertName);
topInsertGenome->copyDimensions(insertGenome);
topInsertGenome->copyTopDimensions(insertGenome);
botInsertGenome->copyBottomDimensions(insertGenome);
topInsertGenome->copySequence(insertGenome);
topInsertGenome->copyTopSegments(insertGenome);
topInsertGenome->copyMetadata(insertGenome);
botInsertGenome->copyBottomSegments(insertGenome);
insertGenome->fixParseInfo();
// Copy the bottom segments for the parent genome from the top alignment.
Genome *parentGenome = mainAlignment->openGenome(parentName);
const Genome *botParentGenome = topAlignment->openGenome(parentName);
botParentGenome->copyBottomDimensions(parentGenome);
botParentGenome->copyBottomSegments(parentGenome);
parentGenome->fixParseInfo();
// Fix the parent's other children as well.
vector<string> allChildren = mainAlignment->getChildNames(parentName);
for (size_t i = 0; i < allChildren.size(); i++)
{
if (allChildren[i] != insertName)
{
Genome *outGenome = mainAlignment->openGenome(allChildren[i]);
const Genome *topSegmentsGenome = topAlignment->openGenome(allChildren[i]);
topSegmentsGenome->copyTopDimensions(outGenome);
topSegmentsGenome->copyTopSegments(outGenome);
outGenome->fixParseInfo();
}
}
// Copy the top segments for the child genome from the bottom alignment.
Genome *childGenome = mainAlignment->openGenome(childName);
const Genome *topChildGenome = botAlignment->openGenome(childName);
topChildGenome->copyTopDimensions(childGenome);
topChildGenome->copyTopSegments(childGenome);
childGenome->fixParseInfo();
// Copy the entire genome for the leaf from the bottom alignment.
Genome *outLeafGenome = mainAlignment->openGenome(leafName);
const Genome *inLeafGenome = botAlignment->openGenome(leafName);
inLeafGenome->copy(outLeafGenome);
if (!noMarkAncestors) {
markAncestorsForUpdate(mainAlignment, insertName);
}
mainAlignment->close();
botAlignment->close();
topAlignment->close();
}
int main(int argc, char *argv[])
{
CLParserPtr optParser = initParser();
string inPath, bottomAlignmentFile, topAlignmentFile, genomeName;
bool noMarkAncestors;
try {
optParser->parseOptions(argc, argv);
inPath = optParser->getArgument<string>("inFile");
bottomAlignmentFile = optParser->getOption<string>("bottomAlignmentFile");
topAlignmentFile = optParser->getOption<string>("topAlignmentFile");
genomeName = optParser->getArgument<string>("genomeName");
noMarkAncestors = optParser->getFlag("noMarkAncestors");
} catch (exception &e) {
optParser->printUsage(cerr);
return 1;
}
AlignmentPtr mainAlignment = openHalAlignment(inPath, optParser);
AlignmentConstPtr bottomAlignment;
AlignmentConstPtr topAlignment;
bool useTopAlignment = mainAlignment->getRootName() != genomeName;
bool useBottomAlignment = mainAlignment->getChildNames(genomeName).size() != 0;
Genome *mainReplacedGenome = mainAlignment->openGenome(genomeName);
if (useTopAlignment) {
// Not a root genome. Can update using a top alignment.
if (topAlignmentFile == "\"\"") {
throw hal_exception("Cannot replace non-root genome without a top "
"alignment file.");
}
topAlignment = openHalAlignment(topAlignmentFile,
optParser);
const Genome *topReplacedGenome = topAlignment->openGenome(genomeName);
topReplacedGenome->copyDimensions(mainReplacedGenome);
topReplacedGenome->copySequence(mainReplacedGenome);
}
if (useBottomAlignment) {
// Not a leaf genome. Can update using a bottom alignment.
if (bottomAlignmentFile == "\"\"") {
throw hal_exception("Cannot replace non-leaf genome without a bottom "
"alignment file.");
}
bottomAlignment = openHalAlignment(bottomAlignmentFile, optParser);
const Genome *botReplacedGenome = bottomAlignment->openGenome(genomeName);
botReplacedGenome->copyDimensions(mainReplacedGenome);
botReplacedGenome->copySequence(mainReplacedGenome);
}
if (!useTopAlignment && !useBottomAlignment) {
throw hal_exception("Root genome is also a leaf genome.");
}
if (useBottomAlignment) {
copyFromBottomAlignment(bottomAlignment, mainAlignment, genomeName);
}
if (useTopAlignment) {
copyFromTopAlignment(topAlignment, mainAlignment, genomeName);
}
// Clear update flag if present, since the genome has just been updated.
MetaData *metaData = mainReplacedGenome->getMetaData();
if (metaData->has("needsUpdate")) {
metaData->set("needsUpdate", "false");
}
if (!noMarkAncestors) {
markAncestorsForUpdate(mainAlignment, genomeName);
}
if (useTopAlignment) {
topAlignment->close();
}
if (useBottomAlignment) {
bottomAlignment->close();
}
mainAlignment->close();
}
