//**********************************************************************************************************************
int GetSeqsCommand::readCount(){
try {
string thisOutputDir = outputDir;
if (outputDir == "") { thisOutputDir += m->hasPath(countfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(countfile));
variables["[extension]"] = m->getExtension(countfile);
string outputFileName = getOutputFileName("count", variables);
ofstream out;
m->openOutputFile(outputFileName, out);
ifstream in;
m->openInputFile(countfile, in);
bool wroteSomething = false;
int selectedCount = 0;
string headers = m->getline(in); m->gobble(in);
out << headers << endl;
string test = headers; vector<string> pieces = m->splitWhiteSpace(test);
string name, rest; int thisTotal; rest = "";
while (!in.eof()) {
if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; }
in >> name; m->gobble(in);
in >> thisTotal; m->gobble(in);
if (pieces.size() > 2) { rest = m->getline(in); m->gobble(in); }
if (m->debug) { m->mothurOut("[DEBUG]: " + name + '\t' + rest + "\n"); }
if (names.count(name) != 0) {
out << name << '\t' << thisTotal << '\t' << rest << endl;
wroteSomething = true;
selectedCount+= thisTotal;
}
}
in.close();
out.close();
//check for groups that have been eliminated
CountTable ct;
if (ct.testGroups(outputFileName)) {
ct.readTable(outputFileName, true, false);
ct.printTable(outputFileName);
}
if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); }
outputTypes["count"].push_back(outputFileName); outputNames.push_back(outputFileName);
m->mothurOut("Selected " + toString(selectedCount) + " sequences from your count file."); m->mothurOutEndLine();
return 0;
}
catch(exception& e) {
m->errorOut(e, "GetSeqsCommand", "readCount");
exit(1);
}
}
int MergeGroupsCommand::processCountFile(DesignMap*& designMap){
try {
CountTable countTable;
if (!countTable.testGroups(countfile)) { m->mothurOut("[ERROR]: your countfile contains no group information, please correct.\n"); m->setControl_pressed(true); return 0; }
//read countTable
countTable.readTable(countfile, true, false);
//fill Groups - checks for "all" and for any typo groups
vector<string> nameGroups = countTable.getNamesOfGroups();
if (Groups.size() == 0) { Groups = nameGroups; }
vector<string> dnamesGroups = designMap->getNamesGroups();
//sanity check
bool error = false;
if (nameGroups.size() == dnamesGroups.size()) { //at least there are the same number
//is every group in counttable also in designmap
for (int i = 0; i < nameGroups.size(); i++) {
if (m->getControl_pressed()) { break; }
if (!util.inUsersGroups(nameGroups[i], dnamesGroups)) { error = true; break; }
}
}
if (error) { m->mothurOut("[ERROR]: Your countfile does not contain the same groups as your design file, please correct\n"); m->setControl_pressed(true); return 0; }
//user selected groups - remove some groups from table
if (Groups.size() != nameGroups.size()) {
for (int i = 0; i < nameGroups.size(); i++) {
if (!util.inUsersGroups(nameGroups[i], Groups)) { countTable.removeGroup(nameGroups[i]); }
}
}
//ask again in case order changed
nameGroups = countTable.getNamesOfGroups();
int numGroups = nameGroups.size();
//create new table
CountTable newTable;
vector<string> treatments = designMap->getCategory();
map<string, vector<int> > clearedMap;
for (int i = 0; i < treatments.size(); i++) {
newTable.addGroup(treatments[i]);
vector<int> temp;
clearedMap[treatments[i]] = temp;
}
treatments = newTable.getNamesOfGroups();
set<string> namesToRemove;
vector<string> namesOfSeqs = countTable.getNamesOfSeqs();
for (int i = 0; i < namesOfSeqs.size(); i++) {
if (m->getControl_pressed()) { break; }
vector<int> thisSeqsCounts = countTable.getGroupCounts(namesOfSeqs[i]);
map<string, vector<int> > thisSeqsMap = clearedMap;
for (int j = 0; j < numGroups; j++) {
thisSeqsMap[designMap->get(nameGroups[j])].push_back(thisSeqsCounts[j]);
}
//create new counts for seq for new table
vector<int> newCounts; int totalAbund = 0;
for (int j = 0; j < treatments.size(); j++){
int abund = mergeAbund(thisSeqsMap[treatments[j]]);
newCounts.push_back(abund); //order matters, add in count for each treatment in new table.
totalAbund += abund;
}
//add seq to new table
if(totalAbund == 0) {
namesToRemove.insert(namesOfSeqs[i]);
}else { newTable.push_back(namesOfSeqs[i], newCounts); }
}
if (error) { m->setControl_pressed(true); return 0; }
//remove sequences zeroed out by median method
if (namesToRemove.size() != 0) {
//print names
ofstream out;
string accnosFile = "accnosFile.temp";
util.openOutputFile(accnosFile, out);
//output to .accnos file
for (set<string>::iterator it = namesToRemove.begin(); it != namesToRemove.end(); it++) {
if (m->getControl_pressed()) { out.close(); util.mothurRemove(accnosFile); return 0; }
out << *it << endl;
}
out.close();
//run remove.seqs
string inputString = "accnos=" + accnosFile + ", fasta=" + fastafile;
m->mothurOut("/******************************************/"); m->mothurOutEndLine();
m->mothurOut("Running command: remove.seqs(" + inputString + ")"); m->mothurOutEndLine();
current->setMothurCalling(true);
Command* removeCommand = new RemoveSeqsCommand(inputString);
removeCommand->execute();
map<string, vector<string> > filenames = removeCommand->getOutputFiles();
delete removeCommand;
current->setMothurCalling(false);
m->mothurOut("/******************************************/"); m->mothurOutEndLine();
util.mothurRemove(accnosFile);
}
string thisOutputDir = outputDir;
if (outputDir == "") { thisOutputDir += util.hasPath(countfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(countfile));
variables["[extension]"] = util.getExtension(countfile);
string outputFileName = getOutputFileName("count", variables);
outputTypes["count"].push_back(outputFileName); outputNames.push_back(outputFileName);
newTable.printTable(outputFileName);
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeGroupsCommand", "processCountFile");
exit(1);
}
}
//**********************************************************************************************************************
SharedCommand::SharedCommand(string option) {
try {
abort = false; calledHelp = false; pickedGroups=false;
allLines = 1;
//allow user to run help
if(option == "help") { help(); abort = true; calledHelp = true; }
else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else {
vector<string> myArray = setParameters();
OptionParser parser(option);
map<string, string> parameters = parser.getParameters();
ValidParameters validParameter;
map<string, string>::iterator it;
//check to make sure all parameters are valid for command
for (it = parameters.begin(); it != parameters.end(); it++) {
if (!validParameter.isValidParameter(it->first, myArray, it->second)) { abort = true; }
}
//if the user changes the input directory command factory will send this info to us in the output parameter
string inputDir = validParameter.valid(parameters, "inputdir");
if (inputDir == "not found"){ inputDir = ""; }
else {
string path;
it = parameters.find("list");
//user has given a template file
if(it != parameters.end()){
path = util.hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["list"] = inputDir + it->second; }
}
it = parameters.find("group");
//user has given a template file
if(it != parameters.end()){
path = util.hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["group"] = inputDir + it->second; }
}
it = parameters.find("count");
//user has given a template file
if(it != parameters.end()){
path = util.hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["count"] = inputDir + it->second; }
}
it = parameters.find("biom");
//user has given a template file
if(it != parameters.end()){
path = util.hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["biom"] = inputDir + it->second; }
}
}
vector<string> tempOutNames;
outputTypes["shared"] = tempOutNames;
outputTypes["group"] = tempOutNames;
outputTypes["map"] = tempOutNames;
//if the user changes the output directory command factory will send this info to us in the output parameter
outputDir = validParameter.valid(parameters, "outputdir"); if (outputDir == "not found"){ outputDir = ""; }
//check for required parameters
listfile = validParameter.validFile(parameters, "list");
if (listfile == "not open") { listfile = ""; abort = true; }
else if (listfile == "not found") { listfile = ""; }
else { current->setListFile(listfile); }
biomfile = validParameter.validFile(parameters, "biom");
if (biomfile == "not open") { biomfile = ""; abort = true; }
else if (biomfile == "not found") { biomfile = ""; }
else { current->setBiomFile(biomfile); }
ordergroupfile = validParameter.validFile(parameters, "ordergroup");
if (ordergroupfile == "not open") { abort = true; }
else if (ordergroupfile == "not found") { ordergroupfile = ""; }
groupfile = validParameter.validFile(parameters, "group");
if (groupfile == "not open") { groupfile = ""; abort = true; }
else if (groupfile == "not found") { groupfile = ""; }
else { current->setGroupFile(groupfile); }
countfile = validParameter.validFile(parameters, "count");
if (countfile == "not open") { countfile = ""; abort = true; }
else if (countfile == "not found") { countfile = ""; }
else {
current->setCountFile(countfile);
CountTable temp;
if (!temp.testGroups(countfile)) {
m->mothurOut("\n[WARNING]: Your count file does not have group info, all reads will be assigned to mothurGroup.\n");
temp.readTable(countfile, false, false); //dont read groups
map<string, int> seqs = temp.getNameMap();
CountTable newCountTable;
newCountTable.addGroup("mothurGroup");
for (map<string, int>::iterator it = seqs.begin(); it != seqs.end(); it++) {
vector<int> counts; counts.push_back(it->second);
newCountTable.push_back(it->first, counts);
}
string newCountfileName = util.getRootName(countfile) + "mothurGroup" + util.getExtension(countfile);
newCountTable.printTable(newCountfileName);
current->setCountFile(newCountfileName);
countfile = newCountfileName;
outputNames.push_back(newCountfileName);
}
}
if ((biomfile == "") && (listfile == "") && (countfile == "")) { //you must provide at least one of the following
//is there are current file available for either of these?
//give priority to list, then biom, then count
listfile = current->getListFile();
if (listfile != "") { m->mothurOut("Using " + listfile + " as input file for the list parameter.\n"); }
else {
biomfile = current->getBiomFile();
if (biomfile != "") { m->mothurOut("Using " + biomfile + " as input file for the biom parameter.\n"); }
else {
countfile = current->getCountFile();
if (countfile != "") { m->mothurOut("Using " + countfile + " as input file for the count parameter.\n"); }
else {
m->mothurOut("[ERROR]: No valid current files. You must provide a list or biom or count file before you can use the make.shared command.\n"); abort = true;
}
}
}
}
else if ((biomfile != "") && (listfile != "")) { m->mothurOut("When executing a make.shared command you must enter ONLY ONE of the following: list or biom.\n"); abort = true; }
if (listfile != "") {
if ((groupfile == "") && (countfile == "")) {
groupfile = current->getGroupFile();
if (groupfile != "") { m->mothurOut("Using " + groupfile + " as input file for the group parameter.\n"); }
else {
countfile = current->getCountFile();
if (countfile != "") { m->mothurOut("Using " + countfile + " as input file for the count parameter.\n"); }
else { m->mothurOut("[ERROR]: You need to provide a groupfile or countfile if you are going to use the list format.\n"); abort = true; }
}
}
}
string groups = validParameter.valid(parameters, "groups");
if (groups == "not found") { groups = ""; }
else {
pickedGroups=true;
util.splitAtDash(groups, Groups);
if (Groups.size() != 0) { if (Groups[0]== "all") { Groups.clear(); } }
}
//check for optional parameter and set defaults
// ...at some point should added some additional type checking...
string label = validParameter.valid(parameters, "label");
if (label == "not found") { label = ""; }
else {
if(label != "all") { util.splitAtDash(label, labels); allLines = 0; }
else { allLines = 1; }
}
if ((listfile == "") && (biomfile == "") && (countfile != "")) { //building a shared file from a count file, require label
if (labels.size() == 0) {
m->mothurOut("[ERROR]: You must provide a label when converting a count file to a shared file, please correct.\n"); abort = true;
}
}
}
}
catch(exception& e) {
m->errorOut(e, "SharedCommand", "SharedCommand");
exit(1);
}
}
//**********************************************************************************************************************
int RemoveRareCommand::processList(){
try {
//you must provide a label because the names in the listfile need to be consistent
string thisLabel = "";
if (allLines) { m->mothurOut("For the listfile you must select one label, using first label in your listfile."); m->mothurOutEndLine(); }
else if (labels.size() > 1) { m->mothurOut("For the listfile you must select one label, using " + (*labels.begin()) + "."); m->mothurOutEndLine(); thisLabel = *labels.begin(); }
else { thisLabel = *labels.begin(); }
InputData input(listfile, "list");
ListVector* list = input.getListVector();
//get first one or the one we want
if (thisLabel != "") {
//use smart distancing
set<string> userLabels; userLabels.insert(thisLabel);
set<string> processedLabels;
string lastLabel = list->getLabel();
while((list != NULL) && (userLabels.size() != 0)) {
if(userLabels.count(list->getLabel()) == 1){
processedLabels.insert(list->getLabel());
userLabels.erase(list->getLabel());
break;
}
if ((m->anyLabelsToProcess(list->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
processedLabels.insert(list->getLabel());
userLabels.erase(list->getLabel());
delete list;
list = input.getListVector(lastLabel);
break;
}
lastLabel = list->getLabel();
delete list;
list = input.getListVector();
}
if (userLabels.size() != 0) {
m->mothurOut("Your file does not include the label " + thisLabel + ". I will use " + lastLabel + "."); m->mothurOutEndLine();
list = input.getListVector(lastLabel);
}
}
string thisOutputDir = outputDir;
if (outputDir == "") { thisOutputDir += m->hasPath(listfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(listfile));
variables["[extension]"] = m->getExtension(listfile);
variables["[tag]"] = list->getLabel();
string outputFileName = getOutputFileName("list", variables);
variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(groupfile));
variables["[extension]"] = m->getExtension(groupfile);
string outputGroupFileName = getOutputFileName("group", variables);
variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(countfile));
variables["[extension]"] = m->getExtension(countfile);
string outputCountFileName = getOutputFileName("count", variables);
ofstream out, outGroup;
m->openOutputFile(outputFileName, out);
bool wroteSomething = false;
//if groupfile is given then use it
GroupMap* groupMap;
CountTable ct;
if (groupfile != "") {
groupMap = new GroupMap(groupfile); groupMap->readMap();
SharedUtil util;
vector<string> namesGroups = groupMap->getNamesOfGroups();
util.setGroups(Groups, namesGroups);
m->openOutputFile(outputGroupFileName, outGroup);
}else if (countfile != "") {
ct.readTable(countfile, true, false);
if (ct.hasGroupInfo()) {
vector<string> namesGroups = ct.getNamesOfGroups();
SharedUtil util;
util.setGroups(Groups, namesGroups);
}
}
if (list != NULL) {
vector<string> binLabels = list->getLabels();
vector<string> newLabels;
//make a new list vector
ListVector newList;
newList.setLabel(list->getLabel());
//for each bin
for (int i = 0; i < list->getNumBins(); i++) {
if (m->control_pressed) { if (groupfile != "") { delete groupMap; outGroup.close(); m->mothurRemove(outputGroupFileName); } out.close(); m->mothurRemove(outputFileName); return 0; }
//parse out names that are in accnos file
string binnames = list->get(i);
vector<string> names;
string saveBinNames = binnames;
m->splitAtComma(binnames, names);
int binsize = names.size();
vector<string> newGroupFile;
if (groupfile != "") {
vector<string> newNames;
saveBinNames = "";
for(int k = 0; k < names.size(); k++) {
string group = groupMap->getGroup(names[k]);
if (m->inUsersGroups(group, Groups)) {
newGroupFile.push_back(names[k] + "\t" + group);
newNames.push_back(names[k]);
saveBinNames += names[k] + ",";
}
}
names = newNames; binsize = names.size();
saveBinNames = saveBinNames.substr(0, saveBinNames.length()-1);
}else if (countfile != "") {
saveBinNames = "";
binsize = 0;
for(int k = 0; k < names.size(); k++) {
if (ct.hasGroupInfo()) {
vector<string> thisSeqsGroups = ct.getGroups(names[k]);
int thisSeqsCount = 0;
for (int n = 0; n < thisSeqsGroups.size(); n++) {
if (m->inUsersGroups(thisSeqsGroups[n], Groups)) {
thisSeqsCount += ct.getGroupCount(names[k], thisSeqsGroups[n]);
}
}
binsize += thisSeqsCount;
//if you don't have any seqs from the groups the user wants, then remove you.
if (thisSeqsCount == 0) { newGroupFile.push_back(names[k]); }
else { saveBinNames += names[k] + ","; }
}else {
binsize += ct.getNumSeqs(names[k]);
saveBinNames += names[k] + ",";
}
}
saveBinNames = saveBinNames.substr(0, saveBinNames.length()-1);
}
if (binsize > nseqs) { //keep bin
newList.push_back(saveBinNames);
newLabels.push_back(binLabels[i]);
if (groupfile != "") { for(int k = 0; k < newGroupFile.size(); k++) { outGroup << newGroupFile[k] << endl; } }
else if (countfile != "") { for(int k = 0; k < newGroupFile.size(); k++) { ct.remove(newGroupFile[k]); } }
}else { if (countfile != "") { for(int k = 0; k < names.size(); k++) { ct.remove(names[k]); } } }
}
//print new listvector
if (newList.getNumBins() != 0) {
wroteSomething = true;
newList.setLabels(newLabels);
newList.printHeaders(out);
newList.print(out);
}
}
out.close();
if (groupfile != "") { outGroup.close(); outputTypes["group"].push_back(outputGroupFileName); outputNames.push_back(outputGroupFileName); }
if (countfile != "") {
if (ct.hasGroupInfo()) {
vector<string> allGroups = ct.getNamesOfGroups();
for (int i = 0; i < allGroups.size(); i++) {
if (!m->inUsersGroups(allGroups[i], Groups)) { ct.removeGroup(allGroups[i]); }
}
}
ct.printTable(outputCountFileName);
outputTypes["count"].push_back(outputCountFileName); outputNames.push_back(outputCountFileName);
}
if (wroteSomething == false) { m->mothurOut("Your file contains only rare sequences."); m->mothurOutEndLine(); }
outputTypes["list"].push_back(outputFileName); outputNames.push_back(outputFileName);
return 0;
}
catch(exception& e) {
m->errorOut(e, "RemoveRareCommand", "processList");
exit(1);
}
}