static Int2 Main_old (void)
{
AsnIoPtr aip, xml_aip = NULL;
BioseqPtr query_bsp, PNTR query_bsp_array;
BioSourcePtr source;
BLAST_MatrixPtr matrix;
BLAST_OptionsBlkPtr options;
BLAST_KarlinBlkPtr ka_params=NULL, ka_params_gap=NULL;
BlastPruneSapStructPtr prune;
Boolean db_is_na, query_is_na, show_gi, believe_query=FALSE;
Boolean html=FALSE;
CharPtr params_buffer=NULL;
Int4 number_of_descriptions, number_of_alignments;
SeqAlignPtr seqalign, PNTR seqalign_array;
SeqAnnotPtr seqannot;
SeqEntryPtr PNTR sepp;
TxDfDbInfoPtr dbinfo=NULL, dbinfo_head;
Uint1 align_type, align_view, out_type;
Uint4 align_options, print_options;
ValNodePtr mask_loc, mask_loc_start, next_mask_loc;
ValNodePtr vnp, other_returns, error_returns;
CharPtr blast_program, blast_database, blast_inputfile, blast_outputfile;
FILE *infp, *outfp, *mqfp=NULL;
Int4 index, num_bsps, total_length, total_processed = 0;
Int2 ctr = 1;
Char prefix[2];
SeqLocPtr last_mask, mask_slp;
Boolean done, hits_found;
Boolean lcase_masking;
MBXmlPtr mbxp = NULL;
Boolean traditional_formatting;
blast_program = "blastn";
blast_database = myargs [ARG_DB].strvalue;
blast_inputfile = myargs [ARG_QUERY].strvalue;
blast_outputfile = myargs [ARG_OUT].strvalue;
if (myargs[ARG_HTML].intvalue)
html = TRUE;
if ((infp = FileOpen(blast_inputfile, "r")) == NULL) {
ErrPostEx(SEV_FATAL, 1, 0, "mgblast: Unable to open input file %s\n", blast_inputfile);
return (1);
}
align_view = (Int1) myargs[ARG_FORMAT].intvalue;
/* Geo mod:
-- replaced myargs[ARG_OUTTYPE].intvalue with out_type from now on
*/
out_type=(Int1) myargs[ARG_OUTTYPE].intvalue;
if (out_type==MGBLAST_FLTHITS || out_type==MGBLAST_HITGAPS) {
align_view = 12 + (out_type-MGBLAST_FLTHITS );
out_type=MBLAST_ALIGNMENTS;
//Attention: 12 MUST be the -m mgblast tab option for MGBLAST_FLTHITS format
// and MGBLAST_HITGAPS = MGBLAST_FLTHITS+1
if (align_view>12) { // this is MGBLAST_HITGAPS output
gap_Info=TRUE;
if (dbgaps_buf==NULL)
dbgaps_buf=(CharPtr) Malloc(dbgaps_bufsize + 1);
if (qgaps_buf==NULL)
qgaps_buf=(CharPtr) Malloc(qgaps_bufsize + 1);
}
}
outfp = NULL;
traditional_formatting =
(out_type == MBLAST_ALIGNMENTS ||
out_type == MBLAST_DELAYED_TRACEBACK);
if ((!traditional_formatting ||
(align_view != 7 && align_view != 10 && align_view != 11)) &&
blast_outputfile != NULL) {
if ((outfp = FileOpen(blast_outputfile, "w")) == NULL) {
ErrPostEx(SEV_FATAL, 1, 0, "blast: Unable to open output file %s\n", blast_outputfile);
return (1);
}
}
//align_type = BlastGetTypes(blast_program, &query_is_na, &db_is_na);
align_type = BlastGetTypes(blast_program, &query_is_na, &db_is_na);
/*
if (!traditional_formatting)
believe_query = TRUE;
else
believe_query = (Boolean) myargs[ARG_BELIEVEQUERY].intvalue;
*/
//Geo mod:
believe_query=FALSE;
//If ASN.1 output is requested and believe_query is not set to TRUE,
// exit with an error.
if (!believe_query && (myargs[ARG_ASNOUT].strvalue ||
align_view == 10 || align_view == 11)) {
ErrPostEx(SEV_FATAL, 1, 0,
"-J option must be TRUE to produce ASN.1 output; before "
"changing -J to TRUE please also ensure that all query "
"sequence identifiers are unique");
return -1;
}
options = BLASTOptionNewEx(blast_program, TRUE, TRUE);
if (options == NULL)
return 3;
options->do_sum_stats = FALSE;
options->is_neighboring = FALSE;
options->expect_value = (Nlm_FloatHi) myargs [ARG_EVALUE].floatvalue;
number_of_descriptions = myargs[ARG_DESCRIPTIONS].intvalue;
number_of_alignments = myargs[ARG_ALIGNMENTS].intvalue;
options->hitlist_size = MAX(number_of_descriptions, number_of_alignments);
if (myargs[ARG_XDROP].intvalue != 0)
options->gap_x_dropoff = myargs[ARG_XDROP].intvalue;
if (myargs[ARG_XDROP_UNGAPPED].intvalue != 0)
options->dropoff_2nd_pass = myargs[ARG_XDROP_UNGAPPED].intvalue;
if (myargs[ARG_XDROP_FINAL].intvalue != 0)
options->gap_x_dropoff_final = myargs[ARG_XDROP_FINAL].intvalue;
if (StringICmp(myargs[ARG_FILTER].strvalue, "T") == 0)
options->filter_string = StringSave("D");
else
options->filter_string = StringSave(myargs[ARG_FILTER].strvalue);
show_gi = (Boolean) myargs[ARG_SHOWGIS].intvalue;
options->penalty = myargs[ARG_MISMATCH].intvalue;
options->reward = myargs[ARG_MATCH].intvalue;
if (myargs[ARG_GAPOPEN].intvalue >= 0)
options->gap_open = myargs[ARG_GAPOPEN].intvalue;
if (myargs[ARG_GAPEXT].intvalue >= 0)
options->gap_extend = myargs[ARG_GAPEXT].intvalue;
if (options->gap_open == 0 && options->reward % 2 == 0 &&
options->gap_extend == options->reward / 2 - options->penalty)
/* This is the default value */
options->gap_extend = 0;
options->genetic_code = 1;
options->db_genetic_code = 1; /* Default; it's not needed here anyway */
options->number_of_cpus = myargs[ARG_THREADS].intvalue;
if (myargs[ARG_WORDSIZE].intvalue != 0)
options->wordsize = myargs[ARG_WORDSIZE].intvalue;
if (myargs[ARG_MINSCORE].intvalue == 0)
options->cutoff_s2 = options->wordsize*options->reward;
else
options->cutoff_s2 = myargs[ARG_MINSCORE].intvalue;
options->db_length = (Int8) myargs[ARG_DBSIZE].floatvalue;
options->searchsp_eff = (Nlm_FloatHi) myargs[ARG_SEARCHSP].floatvalue;
options->perform_culling = FALSE;
/* Kludge */
options->block_width = myargs[ARG_MAXPOS].intvalue;
options->strand_option = myargs[ARG_STRAND].intvalue;
options->window_size = myargs[ARG_WINDOW].intvalue;
#ifdef DO_NOT_SUPPRESS_BLAST_OP
options->mb_template_length = myargs[ARG_TEMPL_LEN].intvalue;
if (myargs[ARG_TEMPL_LEN].intvalue != 0)
options->mb_one_base_step = (Boolean) myargs[ARG_EVERYBASE].intvalue;
options->mb_disc_type = myargs[ARG_TEMPL_TYPE].intvalue;
#endif
lcase_masking = (Boolean) myargs[ARG_LCASE].intvalue;
/* Allow dynamic programming gapped extension only with affine
gap scores */
if (options->gap_open != 0 || options->gap_extend != 0)
options->mb_use_dyn_prog = (Boolean) myargs[ARG_DYNAMIC].intvalue;
print_options = 0;
align_options = 0;
align_options += TXALIGN_COMPRESS;
align_options += TXALIGN_END_NUM;
if (show_gi) {
align_options += TXALIGN_SHOW_GI;
print_options += TXALIGN_SHOW_GI;
}
if (align_view) {
align_options += TXALIGN_MASTER;
if (align_view == 1 || align_view == 3)
align_options += TXALIGN_MISMATCH;
if (align_view == 3 || align_view == 4 || align_view == 6)
align_options += TXALIGN_FLAT_INS;
if (align_view == 5 || align_view == 6)
align_options += TXALIGN_BLUNT_END;
} else {
align_options += TXALIGN_MATRIX_VAL;
align_options += TXALIGN_SHOW_QS;
}
if (html) {
align_options += TXALIGN_HTML;
print_options += TXALIGN_HTML;
}
if (myargs[ARG_GILIST].strvalue)
options->gifile = StringSave(myargs[ARG_GILIST].strvalue);
if (out_type == MBLAST_ENDPOINTS)
options->no_traceback = 1;
else if (out_type == MBLAST_DELAYED_TRACEBACK)
options->no_traceback = 2;
else
options->no_traceback = 0;
options->megablast_full_deflines = (Boolean) myargs[ARG_FULLID].intvalue;
options->perc_identity = (FloatLo) myargs[ARG_PERC_IDENT].floatvalue;
options->hsp_num_max = myargs[ARG_MAXHSP].intvalue;
if (!believe_query)
options->megablast_full_deflines = TRUE;
/*if (options->megablast_full_deflines)
believe_query = FALSE;*/
query_bsp_array = (BioseqPtr PNTR) MemNew((MAX_NUM_QUERIES+1)*sizeof(BioseqPtr));
sepp = (SeqEntryPtr PNTR) MemNew(MAX_NUM_QUERIES*sizeof(SeqEntryPtr));
StrCpy(prefix, "");
global_fp = outfp;
options->output = outfp;
if (traditional_formatting) {
if (align_view < 7) {
if (html) {
fprintf(outfp, "<HTML>\n<TITLE>MEGABLAST Search Results</TITLE>\n");
fprintf(outfp, "<BODY BGCOLOR=\"#FFFFFF\" LINK=\"#0000FF\" "
"VLINK=\"#660099\" ALINK=\"#660099\">\n");
fprintf(outfp, "<PRE>\n");
}
init_buff_ex(90);
BlastPrintVersionInfo("mgblast", html, outfp);
fprintf(outfp, "\n");
MegaBlastPrintReference(html, 90, outfp);
fprintf(outfp, "\n");
if(!PrintDbInformation(blast_database, !db_is_na, 70, outfp, html))
return 1;
free_buff();
#ifdef OS_UNIX
fprintf(global_fp, "%s", "Searching");
#endif
}
}
aip = NULL;
if (myargs[ARG_ASNOUT].strvalue != NULL) {
if ((aip = AsnIoOpen (myargs[ARG_ASNOUT].strvalue,"w")) == NULL) {
ErrPostEx(SEV_FATAL, 1, 0, "blast: Unable to open output file %s\n", myargs[ARG_ASNOUT].strvalue);
return 1;
}
}
else if (align_view == 10 || align_view == 11)
{
const char* mode = (align_view == 10) ? "w" : "wb";
if ((aip = AsnIoOpen (blast_outputfile, (char*) mode)) == NULL) {
ErrPostEx(SEV_FATAL, 1, 0, "blast: Unable to open output file %s\n", blast_outputfile);
return 1;
}
}
if (align_view == 7) {
xml_aip = AsnIoOpen(blast_outputfile, "wx");
}
if (myargs[ARG_QUERYLOC].strvalue) {
Int4 start, end;
Megablast_GetLoc(myargs[ARG_QUERYLOC].strvalue, &start, &end);
options->required_start = start - 1;
options->required_end = end -1;
}
done = FALSE;
while (!done) {
num_bsps = 0;
total_length = 0;
done = TRUE;
SeqMgrHoldIndexing(TRUE);
mask_slp = last_mask = NULL;
while ((sepp[num_bsps]=FastaToSeqEntryForDb(infp, query_is_na, NULL,
believe_query, prefix, &ctr,
&mask_slp)) != NULL) {
if (!lcase_masking) /* Lower case ignored */
mask_slp = SeqLocFree(mask_slp);
if (mask_slp) {
if (!last_mask)
options->query_lcase_mask = last_mask = mask_slp;
else {
last_mask->next = mask_slp;
last_mask = last_mask->next;
}
mask_slp = NULL;
}
query_bsp = NULL;
SeqEntryExplore(sepp[num_bsps], &query_bsp, FindNuc);
//debug:
/*
char query_buffer[255];
SeqIdWrite(query_bsp->id, query_buffer, PRINTID_FASTA_LONG, BUFFER_LENGTH);
fprintf(stderr, "===> query_buf=%s\n", query_buffer);
*/
if (query_bsp == NULL) {
ErrPostEx(SEV_FATAL, 1, 0, "Unable to obtain bioseq\n");
return 2;
}
source = BioSourceNew();
source->org = OrgRefNew();
source->org->orgname = OrgNameNew();
source->org->orgname->gcode = options->genetic_code;
ValNodeAddPointer(&(query_bsp->descr), Seq_descr_source, source);
query_bsp_array[num_bsps++] = query_bsp;
total_length += query_bsp->length;
if (total_length > myargs[ARG_MAXQUERY].intvalue ||
num_bsps >= MAX_NUM_QUERIES) {
done = FALSE;
break;
}
}
if (num_bsps == 0)
break;
SeqMgrHoldIndexing(FALSE);
other_returns = NULL;
error_returns = NULL;
if (out_type==MBLAST_ENDPOINTS)
seqalign_array = BioseqMegaBlastEngine(query_bsp_array, blast_program,
blast_database, options,
&other_returns, &error_returns,
dummy_callback, NULL, NULL, 0,
MegaBlastPrintEndpoints);
else if (out_type==MBLAST_SEGMENTS)
seqalign_array = BioseqMegaBlastEngine(query_bsp_array, blast_program,
blast_database, options,
&other_returns, &error_returns,
dummy_callback, NULL, NULL, 0,
MegaBlastPrintSegments);
else if (out_type==MBLAST_ALIGN_INFO) {
/* -- Geo mod: do not print header
PrintTabularOutputHeader(blast_database,
(num_bsps==1) ? query_bsp_array[0] : NULL,
NULL, "megablast", 0, believe_query,
global_fp);*/
seqalign_array = BioseqMegaBlastEngine(query_bsp_array, blast_program,
blast_database, options,
&other_returns, &error_returns,
dummy_callback, NULL, NULL, 0,
MegaBlastPrintAlignInfo);
} else if (out_type==MBLAST_ALIGNMENTS) {
seqalign_array = BioseqMegaBlastEngine(query_bsp_array, blast_program,
blast_database, options, &other_returns,
&error_returns, align_view < 7 ? tick_callback : NULL,
NULL, NULL, 0, NULL);
}
#ifdef OS_UNIX
fflush(global_fp);
#endif
if (error_returns) {
BlastErrorPrint(error_returns);
for (vnp = error_returns; vnp; vnp = vnp->next) {
BlastDestroyErrorMessage((BlastErrorMsgPtr)vnp->data.ptrvalue);
}
ValNodeFree(error_returns);
}
if (traditional_formatting) {
dbinfo = NULL;
ka_params = NULL;
ka_params_gap = NULL;
params_buffer = NULL;
mask_loc = NULL;
matrix = NULL;
for (vnp=other_returns; vnp; vnp = vnp->next) {
switch (vnp->choice) {
case TXDBINFO:
dbinfo = vnp->data.ptrvalue;
break;
case TXKABLK_NOGAP:
ka_params = vnp->data.ptrvalue;
break;
case TXKABLK_GAP:
ka_params_gap = vnp->data.ptrvalue;
break;
case TXPARAMETERS:
params_buffer = vnp->data.ptrvalue;
break;
case TXMATRIX:
matrix = vnp->data.ptrvalue;
break;
case SEQLOC_MASKING_NOTSET:
case SEQLOC_MASKING_PLUS1:
case SEQLOC_MASKING_PLUS2:
case SEQLOC_MASKING_PLUS3:
case SEQLOC_MASKING_MINUS1:
case SEQLOC_MASKING_MINUS2:
case SEQLOC_MASKING_MINUS3:
ValNodeAddPointer(&mask_loc, vnp->choice, vnp->data.ptrvalue);
break;
default:
break;
}
}
#ifdef OS_UNIX
if(align_view < 7) {
fprintf(global_fp, "%s\n", " done");
}
#endif
if (myargs[ARG_MASKEDQUERY].strvalue) {
if ((mqfp = FileOpen(myargs[ARG_MASKEDQUERY].strvalue, "w")) == NULL)
ErrPostEx(SEV_WARNING, 1, 0, "Unable to open file %s for masked query\n",
myargs[ARG_MASKEDQUERY].strvalue);
}
hits_found = FALSE;
mask_loc_start = next_mask_loc = mask_loc;
mask_loc = NULL;
if (align_view == 7) {
mbxp = PSIXmlInit(xml_aip, "megablast", blast_database,
options, query_bsp_array[0], 0);
}
if (seqalign_array) { //results returned back for processing
ReadDBBioseqFetchEnable ("megablast", blast_database, db_is_na, TRUE);
for (index=0; index<num_bsps; index++) {
seqalign = seqalign_array[index];
if (next_mask_loc &&
SeqIdComp(SeqLocId((SeqLocPtr)next_mask_loc->data.ptrvalue),
query_bsp_array[index]->id) == SIC_YES) {
mask_loc = (SeqLocPtr)
MemDup(next_mask_loc, sizeof(SeqLoc));
next_mask_loc = next_mask_loc->next;
mask_loc->next = NULL;
}
if (mqfp) {
/* convert mask locations from all sources into
a single seqloc */
mask_slp = NULL;
if (mask_loc)
mask_slp = blastMergeFilterLocs(mask_slp,
(SeqLocPtr)mask_loc->data.ptrvalue,
FALSE, 0, 0);
PrintMaskedSequence(query_bsp_array[index], mask_slp,
mqfp, 50, lcase_masking);
SeqLocSetFree(mask_slp);
}
if (seqalign==NULL) {
mask_loc = MemFree(mask_loc);
continue;
}
hits_found = TRUE;
if (align_view < 7) {
init_buff_ex(70);
AcknowledgeBlastQuery(query_bsp_array[index], 70, outfp,
believe_query, html);
free_buff();
}
if (align_view == 8 || align_view == 9) {
if (align_view == 9)
PrintTabularOutputHeader(blast_database,
query_bsp_array[index], NULL, blast_program, 0,
believe_query, global_fp);
/* debug:
char qbuf[512];
strcpy(qbuf, BioseqGetTitle(query_bsp_array[index]));
fprintf(stderr, "---> Here: query title=%s\n", qbuf);
*/
BlastPrintTabulatedResults(seqalign,
query_bsp_array[index], NULL, number_of_alignments,
blast_program, !options->gapped_calculation,
believe_query, 0, 0,
global_fp, (align_view == 9));
ObjMgrFreeCache(0);
SeqAlignSetFree(seqalign);
mask_loc = MemFree(mask_loc);
continue;
}
//Geo mod:
else if (align_view>=12) {
MGBlastPrintTab(seqalign,
query_bsp_array[index], number_of_alignments,
!options->gapped_calculation,
global_fp);
ObjMgrFreeCache(0);
SeqAlignSetFree(seqalign);
mask_loc = MemFree(mask_loc);
continue;
}
else if(align_view == 7) {
IterationPtr iterp;
iterp = BXMLBuildOneQueryIteration(seqalign,
NULL, FALSE,
!options->gapped_calculation, index,
NULL, query_bsp_array[index], mask_loc);
IterationAsnWrite(iterp, mbxp->aip, mbxp->atp);
AsnIoFlush(mbxp->aip);
IterationFree(iterp);
SeqAlignSetFree(seqalign);
mask_loc = MemFree(mask_loc);
continue;
}
seqannot = SeqAnnotNew();
seqannot->type = 2;
AddAlignInfoToSeqAnnot(seqannot, align_type);
seqannot->data = seqalign;
if (aip) {
SeqAnnotAsnWrite((SeqAnnotPtr) seqannot, aip, NULL);
AsnIoReset(aip);
}
if (outfp) { /* Uncacheing causes problems with ordinal nos. vs. gi's. */
prune = BlastPruneHitsFromSeqAlign(seqalign, number_of_descriptions, NULL);
ObjMgrSetHold();
init_buff_ex(85);
PrintDefLinesFromSeqAlign(prune->sap, 80,
outfp, print_options, FIRST_PASS, NULL);
free_buff();
prune = BlastPruneHitsFromSeqAlign(seqalign, number_of_alignments, prune);
seqannot->data = prune->sap;
if (align_view != 0)
ShowTextAlignFromAnnot(seqannot, 60, outfp, NULL,
NULL, align_options, NULL,
mask_loc, NULL);
else
ShowTextAlignFromAnnot(seqannot, 60, outfp, NULL, NULL, align_options, NULL, mask_loc, FormatScoreFunc);
seqannot->data = seqalign;
prune = BlastPruneSapStructDestruct(prune);
ObjMgrClearHold();
ObjMgrFreeCache(0);
}
seqannot = SeqAnnotFree(seqannot);
mask_loc = MemFree(mask_loc);
} /* End loop on seqaligns for different queries */
ReadDBBioseqFetchDisable();
}
if (mbxp != NULL) {
MBXmlClose(mbxp, other_returns, !options->gapped_calculation);
}
if (mqfp)
FileClose(mqfp);
if (!hits_found && align_view < 7)
fprintf(outfp, "\n\n ***** No hits found ******\n\n");
matrix = BLAST_MatrixDestruct(matrix);
if(html)
fprintf(outfp, "<PRE>\n");
init_buff_ex(85);
dbinfo_head = dbinfo;
if(align_view < 7) {
while (dbinfo) {
PrintDbReport(dbinfo, 70, outfp);
dbinfo = dbinfo->next;
}
}
dbinfo_head = TxDfDbInfoDestruct(dbinfo_head);
if (ka_params) {
if(align_view < 7)
PrintKAParameters(ka_params->Lambda, ka_params->K, ka_params->H, 70, outfp, FALSE);
MemFree(ka_params);
}
if (ka_params_gap) {
if(align_view < 7)
PrintKAParameters(ka_params_gap->Lambda, ka_params_gap->K, ka_params_gap->H, 70, outfp, TRUE);
MemFree(ka_params_gap);
}
if(align_view < 7)
PrintTildeSepLines(params_buffer, 70, outfp);
MemFree(params_buffer);
free_buff();
mask_loc = mask_loc_start;
while (mask_loc) {
SeqLocSetFree(mask_loc->data.ptrvalue);
mask_loc = mask_loc->next;
}
ValNodeFree(mask_loc_start);
} else { //not traditional formatting
/* Just destruct all other_returns parts */
for (vnp=other_returns; vnp; vnp = vnp->next) {
switch (vnp->choice) {
case TXDBINFO:
TxDfDbInfoDestruct(vnp->data.ptrvalue);
break;
case TXKABLK_NOGAP:
case TXKABLK_GAP:
case TXPARAMETERS:
MemFree(vnp->data.ptrvalue);
break;
case TXMATRIX:
BLAST_MatrixDestruct(vnp->data.ptrvalue);
break;
case SEQLOC_MASKING_NOTSET:
case SEQLOC_MASKING_PLUS1:
case SEQLOC_MASKING_PLUS2:
case SEQLOC_MASKING_PLUS3:
case SEQLOC_MASKING_MINUS1:
case SEQLOC_MASKING_MINUS2:
case SEQLOC_MASKING_MINUS3:
mask_loc = vnp->data.ptrvalue;
SeqLocSetFree(mask_loc);
default:
break;
}
}
}
other_returns = ValNodeFree(other_returns);
MemFree(seqalign_array);
options->query_lcase_mask =
SeqLocSetFree(options->query_lcase_mask);
/* Freeing SeqEntries can be very expensive, do this only if
this is not the last iteration of search */
if (!done) {
for (index=0; index<num_bsps; index++) {
sepp[index] = SeqEntryFree(sepp[index]);
query_bsp_array[index] = NULL;
}
}
total_processed += num_bsps;
} /* End of loop on complete searches */
aip = AsnIoClose(aip);
/*if (align_view == 7)
xml_aip = AsnIoClose(xml_aip);*/
if (align_view < 7 && html)
fprintf(outfp, "</PRE>\n</BODY>\n</HTML>\n");
if (align_view < 7 && myargs[ARG_LOGINFO].intvalue)
fprintf(outfp, "Mega BLAST run finished, processed %d queries\n",
total_processed);
MemFree(query_bsp_array);
MemFree(sepp);
MemFree(qgaps_buf);
MemFree(dbgaps_buf);
options = BLASTOptionDelete(options);
FileClose(infp);
FileClose(outfp);
return 0;
}
Int2 Main_old (void)
{
AsnIoPtr aip;
BioseqPtr fake_bsp = NULL, fake_subject_bsp = NULL, query_bsp = NULL,
subject_bsp = NULL;
BioseqPtr bsp1, bsp2;
BLAST_KarlinBlkPtr ka_params=NULL, ka_params_gap=NULL;
BLAST_OptionsBlkPtr options=NULL;
Boolean seq1_is_na, seq2_is_na;
CharPtr params_buffer=NULL;
DbtagPtr dbtagptr;
Uint1 align_type;
Uint4 align_options;
SeqAlignPtr seqalign;
SeqAnnotPtr seqannot;
SeqEntryPtr sep = NULL, sep1 = NULL;
CharPtr program_name, blast_outputfile;
FILE *outfp;
ValNodePtr mask_loc, mask_loc_start, vnp, other_returns=NULL, error_returns=NULL;
BLAST_MatrixPtr matrix;
Int4Ptr PNTR txmatrix;
int (LIBCALLBACK *handle_results)PROTO((VoidPtr search)) = NULL;
Boolean entrez_lookup = FALSE;
Boolean html, seqannot_output, believe_query;
Uint1 tabular_output;
Boolean gapped_calculation;
entrez_lookup = (Boolean) myargs[ARG_ACCN].intvalue;
html = (Boolean) myargs[ARG_HTML].intvalue;
seqannot_output = (myargs[ARG_ASNOUT].strvalue != NULL);
blast_outputfile = myargs [ARG_OUT].strvalue;
program_name = StringSave(myargs[ARG_PROGRAM].strvalue);
if (StringCmp(program_name, "blastn") &&
StringCmp(program_name, "blastp") &&
StringCmp(program_name, "blastx") &&
StringCmp(program_name, "tblastn") &&
StringCmp(program_name, "tblastx")) {
ErrPostEx(SEV_FATAL, 1, 0, "Program name must be blastn, blastp, blastx, tblastn or tblastx\n");
return (1);
}
align_type = BlastGetTypes(program_name, &seq1_is_na, &seq2_is_na);
if ((outfp = FileOpen(blast_outputfile, "w")) == NULL)
{
ErrPostEx(SEV_FATAL, 1, 0, "blast: Unable to open output file %s\n", blast_outputfile);
return (1);
}
gapped_calculation = (Boolean) myargs[ARG_GAPPED].intvalue;
believe_query = (seqannot_output || entrez_lookup);
options = BLASTOptionNewEx(program_name, gapped_calculation,
(Boolean) myargs[ARG_USEMEGABLAST].intvalue);
if (BL2SEQ_GetSequences(seq1_is_na, seq2_is_na, &query_bsp, &subject_bsp,
&sep, &sep1, &(options->query_lcase_mask),
believe_query) == FALSE)
{
ErrPostEx(SEV_FATAL, 1, 0, "blast: Unable to get sequences");
return (1);
}
if (!entrez_lookup) {
if (!believe_query)
fake_bsp = BlastMakeFakeBioseq(query_bsp, NULL);
fake_subject_bsp = BioseqNew();
fake_subject_bsp->descr = subject_bsp->descr;
fake_subject_bsp->repr = subject_bsp->repr;
fake_subject_bsp->mol = subject_bsp->mol;
fake_subject_bsp->length = subject_bsp->length;
fake_subject_bsp->seq_data = subject_bsp->seq_data;
fake_subject_bsp->seq_data_type = subject_bsp->seq_data_type;
dbtagptr = DbtagNew();
dbtagptr->db = StringSave("BL_ORD_ID");
dbtagptr->tag = ObjectIdNew();
if (BioseqGetTitle(subject_bsp) != NULL)
dbtagptr->tag->str = StringSave(BioseqGetTitle(subject_bsp));
else
dbtagptr->tag->str = StringSave("No definition line found");
ValNodeAddPointer(&fake_subject_bsp->id, SEQID_GENERAL, dbtagptr);
bsp1 = (believe_query ? query_bsp : fake_bsp);
bsp2 = fake_subject_bsp;
} else {
bsp1 = query_bsp;
bsp2 = subject_bsp;
}
tabular_output = (Uint1) myargs[ARG_FORMAT].intvalue;
if (myargs[ARG_SEARCHSP].floatvalue)
options->searchsp_eff = (Nlm_FloatHi) myargs[ARG_SEARCHSP].floatvalue;
options->filter_string = StringSave(myargs[ARG_FILTER].strvalue);
options->expect_value = (Nlm_FloatHi) myargs [ARG_EVALUE].floatvalue;
if (StringICmp("blastn", program_name) == 0)
{
options->penalty = myargs[ARG_MISMATCH].intvalue;
options->reward = myargs[ARG_MATCH].intvalue;
}
options->db_length = (Int8) myargs[ARG_DBSIZE].floatvalue;
options->discontinuous = FALSE;
if (myargs[ARG_XDROP].intvalue != 0)
{
options->gap_x_dropoff = myargs[ARG_XDROP].intvalue;
}
if (myargs[ARG_WORDSIZE].intvalue != 0)
options->wordsize = (Int2) myargs[ARG_WORDSIZE].intvalue;
if (options->is_megablast_search) {
options->cutoff_s2 = options->wordsize*options->reward;
}
options->matrix = MemFree(options->matrix);
BLASTOptionSetGapParams(options, myargs[ARG_MATRIX].strvalue, 0, 0);
if (myargs[ARG_GAPOPEN].intvalue != -1)
options->gap_open = myargs[ARG_GAPOPEN].intvalue;
if (myargs[ARG_GAPEXT].intvalue != -1)
options->gap_extend = myargs[ARG_GAPEXT].intvalue;
options->strand_option = myargs[ARG_STRAND].intvalue;
/* Input longest intron length is in nucleotide scale; in the lower
level code it will be used in protein scale */
if (myargs[ARG_INTRON].intvalue > 0)
options->longest_intron = myargs[ARG_INTRON].intvalue;
if (!myargs[ARG_LOC1].strvalue && !myargs[ARG_LOC2].strvalue) {
seqalign = BlastTwoSequencesWithCallback(bsp1, bsp2, program_name,
options, &other_returns, &error_returns, handle_results);
} else {
SeqLocPtr slp1=NULL, slp2=NULL;
if (BL2SEQ_MakeSeqLoc(bsp1, bsp2, &slp1, &slp2, options->strand_option) == FALSE)
return 1;
seqalign = BlastTwoSequencesByLocWithCallback(slp1, slp2, program_name, options, &other_returns, &error_returns, handle_results, NULL);
SeqLocFree(slp1);
SeqLocFree(slp2);
}
if (error_returns) {
BlastErrorPrint(error_returns);
for (vnp = error_returns; vnp; vnp = vnp->next) {
BlastDestroyErrorMessage((BlastErrorMsgPtr)vnp->data.ptrvalue);
}
ValNodeFree(error_returns);
}
ka_params = NULL;
ka_params_gap = NULL;
params_buffer = NULL;
mask_loc = NULL;
matrix = NULL;
txmatrix = NULL;
for (vnp=other_returns; vnp; vnp = vnp->next) {
switch (vnp->choice) {
case TXKABLK_NOGAP:
ka_params = vnp->data.ptrvalue;
break;
case TXKABLK_GAP:
ka_params_gap = vnp->data.ptrvalue;
break;
case TXPARAMETERS:
params_buffer = vnp->data.ptrvalue;
break;
case TXMATRIX:
matrix = vnp->data.ptrvalue;
if (matrix && !tabular_output)
txmatrix = BlastMatrixToTxMatrix(matrix);
break;
case SEQLOC_MASKING_NOTSET:
case SEQLOC_MASKING_PLUS1:
case SEQLOC_MASKING_PLUS2:
case SEQLOC_MASKING_PLUS3:
case SEQLOC_MASKING_MINUS1:
case SEQLOC_MASKING_MINUS2:
case SEQLOC_MASKING_MINUS3:
ValNodeAddPointer(&mask_loc, vnp->choice, vnp->data.ptrvalue);
break;
default:
break;
}
}
if (!tabular_output || seqannot_output) {
align_options = 0;
align_options += TXALIGN_MATRIX_VAL;
align_options += TXALIGN_SHOW_QS;
align_options += TXALIGN_COMPRESS;
align_options += TXALIGN_END_NUM;
if (StringICmp("blastx", program_name) == 0) {
align_options += TXALIGN_BLASTX_SPECIAL;
}
if (html)
align_options += TXALIGN_HTML;
seqannot = SeqAnnotNew();
seqannot->type = 2;
AddAlignInfoToSeqAnnot(seqannot, align_type);
seqannot->data = seqalign;
aip = NULL;
if (seqannot_output)
aip = AsnIoOpen (myargs[ARG_ASNOUT].strvalue,"w");
if (aip && seqannot) {
SeqAnnotAsnWrite((SeqAnnotPtr) seqannot, aip, NULL);
AsnIoReset(aip);
aip = AsnIoClose(aip);
}
}
if (!tabular_output) {
AcknowledgeBlastQuery(query_bsp, 70, outfp, believe_query, html);
ShowTextAlignFromAnnot(seqannot, 60, outfp, NULL, NULL, align_options, txmatrix, mask_loc, FormatScoreFunc);
seqannot = SeqAnnotFree(seqannot);
if (txmatrix)
txmatrix = TxMatrixDestruct(txmatrix);
init_buff_ex(85);
if (ka_params) {
PrintKAParameters(ka_params->Lambda, ka_params->K, ka_params->H, 70, outfp, FALSE);
}
if (ka_params_gap) {
PrintKAParameters(ka_params_gap->Lambda, ka_params_gap->K, ka_params_gap->H, 70, outfp, TRUE);
}
PrintTildeSepLines(params_buffer, 70, outfp);
free_buff();
} else {
PrintTabularOutputHeader(NULL, query_bsp, NULL,
program_name, 0, believe_query, outfp);
BlastPrintTabulatedResults(seqalign, query_bsp, NULL,
1, program_name, !gapped_calculation,
believe_query, 0, 0, outfp, FALSE);
SeqAlignSetFree(seqalign);
}
matrix = BLAST_MatrixDestruct(matrix);
MemFree(ka_params);
MemFree(ka_params_gap);
MemFree(params_buffer);
mask_loc_start = mask_loc;
while (mask_loc) {
SeqLocSetFree(mask_loc->data.ptrvalue);
mask_loc = mask_loc->next;
}
ValNodeFree(mask_loc_start);
fake_bsp = BlastDeleteFakeBioseq(fake_bsp);
other_returns = ValNodeFree(other_returns);
options->query_lcase_mask = SeqLocSetFree(options->query_lcase_mask);
options = BLASTOptionDelete(options);
MemFree(program_name);
FileClose(outfp);
if (entrez_lookup) {
BioseqFree(query_bsp);
BioseqFree(subject_bsp);
} else {
SeqEntryFree(sep);
SeqEntryFree(sep1);
}
return 0;
}
Int2 BLAST_FormatResults(SBlastSeqalignArray* seqalign_arr, Int4 num_queries,
SeqLoc* query_slp, SeqLoc* mask_loc_head,
BlastFormattingInfo* format_info,
Blast_SummaryReturn* sum_returns)
{
SeqLoc* mask_loc;
SeqLoc* next_mask_loc = NULL;
SeqLoc* tmp_loc = NULL;
Uint1 align_type;
Boolean db_is_na;
Int4 query_index;
SeqLoc* slp;
SeqLoc* mask_slp;
AsnIo* aip = NULL;
MBXml* xmlp = NULL;
FILE *outfp = NULL;
BlastFormattingOptions* format_options;
EAlignView align_view;
Boolean ungapped;
ASSERT(format_info && format_info->format_options &&
format_info->search_options && query_slp);
format_options = format_info->format_options;
align_view = format_options->align_view;
ungapped =
!format_info->search_options->score_options->gapped_calculation;
if (align_view == eAlignViewXml) {
const Int4 kXmlFlag = 0; /* Change to BXML_INCLUDE_QUERY if inclusion
of query sequence is desired in the XML
output header. */
xmlp = format_info->xmlp;
if (!xmlp) {
xmlp = format_info->xmlp =
s_MBXmlInit(format_info->aip, format_info->program_name,
format_info->db_name, query_slp, kXmlFlag,
sum_returns->search_params);
}
} else if (align_view == eAlignViewAsnText ||
align_view == eAlignViewAsnBinary)
aip = format_info->aip;
else
outfp = format_info->outfp;
align_type =
GetOldAlignType(format_info->search_options->program, &db_is_na);
if (format_info->db_name) {
/* Enable fetching from the BLAST database. */
ReadDBBioseqFetchEnable ("blast", format_info->db_name, db_is_na, TRUE);
/* If database is translated, set the genetic code for tranlation. */
if (Blast_SubjectIsTranslated(format_info->search_options->program)) {
ReadDBBioseqSetDbGeneticCode(format_info->search_options->
db_options->genetic_code);
}
}
if(format_info->search_options->score_options->is_ooframe) {
ErrPostEx(SEV_WARNING, 0, 0,
"Out-of-frame option selected, Expect values are only approximate and calculated not assuming out-of-frame alignments");
}
slp = query_slp;
mask_loc = mask_loc_head;
for (query_index=0; query_index<seqalign_arr->num_queries && slp; query_index++, slp=slp->next)
{
Bioseq* bsp = NULL;
SeqAlignPtr seqalign = seqalign_arr->array[query_index];
/* Find which query the current SeqAlign is for */
SeqId* query_id = TxGetQueryIdFromSeqAlign(seqalign);
if (seqalign == NULL)
{
if (align_view < eAlignViewXml)
s_AcknowledgeEmptyResults(slp, format_options, format_info, outfp); /* this query has no results. */
else if (align_view == eAlignViewXml)
{
/* Retrieve this query's Bioseq */
Iteration* iterp;
/* Call to TxGetQueryIdFromSeqAlign returned NULL. */
query_id = SeqLocId(slp);
bsp = BioseqLockById(query_id);
iterp = s_XMLBuildOneQueryIteration(NULL, sum_returns, FALSE, ungapped,
query_index+1+format_info->num_formatted,
"No hits found", bsp, NULL);
IterationAsnWrite(iterp, xmlp->aip, xmlp->atp);
AsnIoFlush(xmlp->aip);
IterationFree(iterp);
BioseqUnlock(bsp);
}
else if (align_view == eAlignViewTabularWithComments)
{
query_id = SeqLocId(slp);
bsp = BioseqLockById(query_id);
PrintTabularOutputHeader(format_info->db_name, bsp, NULL,
format_info->program_name,
0, format_options->believe_query, outfp);
BioseqUnlock(bsp);
}
continue;
}
format_info->is_seqalign_null = FALSE; /* reset flag, at least one query has seqalign */
/* Find the masking location for this query. Initialize next_mask_loc
to the current start of the chain, in case nothing for this query
will be found. */
next_mask_loc = mask_loc;
for ( ; mask_loc; mask_loc = mask_loc->next) {
mask_slp = (SeqLoc*) mask_loc->data.ptrvalue;
if (SeqIdComp(query_id, SeqLocId(mask_slp)) == SIC_YES) {
break;
}
}
/* Unlink the masking location for this query and save the next one */
if (mask_loc) {
for (next_mask_loc = mask_loc; next_mask_loc->next;
next_mask_loc = next_mask_loc->next) {
mask_slp = (SeqLoc*) next_mask_loc->next->data.ptrvalue;
if (SeqIdComp(query_id, SeqLocId(mask_slp))
!= SIC_YES) {
break;
}
}
tmp_loc = next_mask_loc;
next_mask_loc = next_mask_loc->next;
tmp_loc->next = NULL;
}
/* On the next iteration we can start from the next query */
/* Retrieve this query's Bioseq */
bsp = BioseqLockById(query_id);
if (align_view < eAlignViewXml) {
if (format_info->head_on_every_query == TRUE)
BLAST_PrintOutputHeader(format_info);
init_buff_ex(70);
AcknowledgeBlastQuery(bsp, 70, outfp,
format_options->believe_query, format_options->html);
free_buff();
if (format_info->head_on_every_query == TRUE)
{
s_BLAST_PrintDatabaseInfo(format_info);
fprintf(format_info->outfp, "%s", "Searching..................................................done\n\n");
}
}
if (align_view == eAlignViewTabular ||
align_view == eAlignViewTabularWithComments) {
if (align_view == eAlignViewTabularWithComments)
PrintTabularOutputHeader(format_info->db_name, bsp, NULL,
format_info->program_name,
0, format_options->believe_query, outfp);
BlastPrintTabulatedResults(seqalign, bsp, NULL,
format_options->number_of_alignments, format_info->program_name,
ungapped, format_options->believe_query, 0, 0,
outfp, (Boolean)(align_view == eAlignViewTabularWithComments));
} else if(align_view == eAlignViewXml) {
Iteration* iterp;
ASSERT(xmlp && xmlp->aip);
/* The index of this "query iteration" is the query_index in the
current formatting round, plus the number of previously formatted
queries. */
iterp =
s_XMLBuildOneQueryIteration(seqalign, sum_returns, FALSE,
ungapped,
query_index+1+format_info->num_formatted,
NULL, bsp, mask_loc);
IterationAsnWrite(iterp, xmlp->aip, xmlp->atp);
AsnIoFlush(xmlp->aip);
IterationFree(iterp);
} else {
SeqAnnot* seqannot = SeqAnnotNew();
seqannot->type = 2;
AddAlignInfoToSeqAnnot(seqannot, align_type);
seqannot->data = seqalign;
if (aip) {
SeqAnnotAsnWrite((SeqAnnot*) seqannot, aip, NULL);
AsnIoReset(aip);
}
if (outfp) {
BlastPruneSapStruct* prune;
Int4** matrix = s_LoadMatrix(sum_returns->search_params->matrix);
ObjMgrSetHold();
init_buff_ex(85);
PrintDefLinesFromSeqAlignEx2(seqalign, 80, outfp,
format_options->print_options, FIRST_PASS, NULL,
format_options->number_of_descriptions, NULL, NULL);
free_buff();
/** @todo FIXME: note that by calling BlastPruneHitsFromSeqAlign
* we're making a COPY of the seqalign to print it out! Clearly
* this could use a better design */
prune = BlastPruneHitsFromSeqAlign(seqalign,
format_options->number_of_alignments, NULL);
seqannot->data = prune->sap;
if(format_info->search_options->score_options->is_ooframe) {
OOFShowBlastAlignment(prune->sap, mask_loc, outfp,
format_options->align_options, NULL);
} else if (align_view != eAlignViewPairwise) {
ShowTextAlignFromAnnot(seqannot, 60, outfp, NULL, NULL,
format_options->align_options, matrix, mask_loc, NULL);
} else {
ShowTextAlignFromAnnot(seqannot, 60, outfp, NULL, NULL,
format_options->align_options, matrix, mask_loc,
FormatScoreFunc);
}
s_DeleteMatrix(matrix);
seqannot->data = seqalign;
prune = BlastPruneSapStructDestruct(prune);
ObjMgrClearHold();
}
/* Set data to NULL, because we do not free Seq-align here. */
seqannot->data = NULL;
seqannot = SeqAnnotFree(seqannot);
}
BioseqUnlock(bsp);
/* Relink the mask locations so chain can be freed in the end.
The 'tmp_loc' variable points to the location that was unlinked. */
if (tmp_loc)
tmp_loc->next = next_mask_loc;
mask_loc = next_mask_loc;
ObjMgrFreeCache(0);
} /* End loop on seqaligns for different queries */
/* close BlastOutput_iterations openned in s_MBXmlInit; Rt ticket # 15135151 */
if((format_info->is_seqalign_null==TRUE) && (align_view == eAlignViewXml)) {
/* extra output only if no hits at all, otherwise "for loop" logic should take care*/
Iteration* iterp;
iterp = IterationNew();
iterp->iter_num = 1;
iterp->stat = s_XMLBuildStatistics(sum_returns, ungapped);
ASSERT(xmlp && xmlp->aip);
IterationAsnWrite(iterp, xmlp->aip, xmlp->atp);
AsnIoFlush(xmlp->aip);
IterationFree(iterp);
}
if (format_info->db_name) {
/* Free the database translation tables, if applicable. */
TransTableFreeAll();
ReadDBBioseqFetchDisable();
}
/* Update the count of the formatted queries. */
format_info->num_formatted += num_queries;
return 0;
}