extern "C" void
invoke_copy_to_stack(uint64_t* stk, uint64_t *end,
uint32_t paramCount, nsXPTCVariant* s)
{
uint64_t *ireg_args = stk;
uint64_t *ireg_end = ireg_args + 8;
double *freg_args = (double *)ireg_end;
double *freg_end = freg_args + 8;
uint64_t *stack_args = (uint64_t *)freg_end;
// leave room for 'that' argument in x0
++ireg_args;
for (uint32_t i = 0; i < paramCount; i++, s++) {
if (s->IsPtrData()) {
alloc_word(ireg_args, stack_args, ireg_end, (uint64_t)s->ptr);
continue;
}
// According to the ABI, integral types that are smaller than 8 bytes
// are to be passed in 8-byte registers or 8-byte stack slots.
switch (s->type) {
case nsXPTType::T_FLOAT:
alloc_float(freg_args, stack_args, freg_end, s->val.f);
break;
case nsXPTType::T_DOUBLE:
alloc_double(freg_args, stack_args, freg_end, s->val.d);
break;
case nsXPTType::T_I8: alloc_word(ireg_args, stk, end, s->val.i8); break;
case nsXPTType::T_I16: alloc_word(ireg_args, stk, end, s->val.i16); break;
case nsXPTType::T_I32: alloc_word(ireg_args, stk, end, s->val.i32); break;
case nsXPTType::T_I64: alloc_word(ireg_args, stk, end, s->val.i64); break;
case nsXPTType::T_U8: alloc_word(ireg_args, stk, end, s->val.u8); break;
case nsXPTType::T_U16: alloc_word(ireg_args, stk, end, s->val.u16); break;
case nsXPTType::T_U32: alloc_word(ireg_args, stk, end, s->val.u32); break;
case nsXPTType::T_U64: alloc_word(ireg_args, stk, end, s->val.u64); break;
case nsXPTType::T_BOOL: alloc_word(ireg_args, stk, end, s->val.b); break;
case nsXPTType::T_CHAR: alloc_word(ireg_args, stk, end, s->val.c); break;
case nsXPTType::T_WCHAR: alloc_word(ireg_args, stk, end, s->val.wc); break;
default:
// all the others are plain pointer types
alloc_word(ireg_args, stack_args, ireg_end,
reinterpret_cast<uint64_t>(s->val.p));
break;
}
}
}
bool *number_to_bin(int i){ /*integer to digital convertion*/
int divisor, quocient,c,tmp;
bool converted,temporary;
bool *word=alloc_word(); /*final word*/
/*now we fill the word with zeroes*/
zero_stream(word,WORD_SIZE);
divisor=i;
converted=false; /*initially, the number is not converted*/
for(c=0;c<=WORD_SIZE;c++){ /*plus one execution (<=), just in case that number ocupe all the WORD_SIZE space*/
if(converted==false){
if((quocient!=1)&&(quocient!=0)){ /*if the process is not in the end*/
quocient=(divisor-(divisor%2))/2;
word[c]=(divisor%2==0)?false:true;
divisor=quocient;
}else{
*(word+c)=(quocient==1)?true:false;
/*this will make the for restart( but now, executing the first else )*/
converted=true;
tmp=c; /*tmp backup the value of c*/
break;
}
}else{ /*this will reverse the order(basic number to digital convertion)*/
if(c<=((tmp-(tmp%2))/2)){ /*if its less or equal the middle/last element*/
/*not exchange the value if its impar and reached the middle element,(tmp starts in 0) and then stop*/
if((tmp%2==0)&&(c==(tmp-(tmp%2))/2)){
break;
}else{
/*this exchange the position*/
temporary=word[c];
word[c]=word[tmp-c];
word[tmp-c]=temporary;
}
}else{
break;
}
}
}
if(tmp!=WORD_SIZE){
/*now we simply shift the number to the top*/
for(c=0;c<=tmp;c++){
word[WORD_SIZE-1-c]=word[tmp-c];
word[tmp-c]=false;
}
}
return word;
}
SEXP GADEM_Analysis(SEXP sequence,SEXP sizeSeq, SEXP accession, SEXP Rverbose,SEXP RnumWordGroup,SEXP RnumTop3mer,SEXP RnumTop4mer,SEXP RnumTop5mer,SEXP RnumGeneration,SEXP RpopulationSize, SEXP RpValue,SEXP ReValue,SEXP RextTrim,SEXP RminSpaceWidth,SEXP RmaxSpaceWidth,SEXP RuseChIPscore,SEXP RnumEM,SEXP RfEM, SEXP RwidthWt,SEXP RfullScan, SEXP RslideWinPWM,SEXP RstopCriterion,SEXP RnumBackgSets,SEXP RweightType,SEXP RbFileName,SEXP RListPWM,SEXP RminSites,SEXP RmaskR,SEXP Rnmotifs)
{
char *bFileName;
SEXP ResultsGadem;
SEXP RSpwm;
PROTECT(ResultsGadem=NEW_LIST(100));
int increment=0;
double testrand;
//Number of sequences
int numSeq = INTEGER_VALUE(sizeSeq);
// const
// char *Fastaheader[size];
int incr=0;
int longueur=length(sequence);
int IncrementTemp=0;
// basic settings/info
int maxSeqLen,*seqLen; // sequence info
double aveSeqLen; // sequence info
char **seq,**rseq;
int *geneID; // sequence info
char **oseq,**orseq; // copy of the original sequences
char **sseq,**rsseq; // simulated seqs.
double *bfreq1, *bfreq0=NULL; // base frequencies
double *ChIPScore; // chip score
int maskR; // mask simple repeats before running the algorithm
// pwms
double ***pwm; // initial population of PWMs from spaced dyads
int *pwmLen; // initial pwm lengths
double **opwm2; // EM-derived PWM
double ***opwm; // observed PWMs from identified sites
double ***epwm; // em-optimized PWMs
double **logepwm; // log(em-optimized PWM)
int *pwmnewLen; // final motif length after extending to both ends
// llr score distr.
Pgfs *llrDist; // llr distribution from pgf method
int llrDim; // llr distribution dimension
int **ipwm; // integer pwm for computing llr score distribution
// EM, motif, sites
double pvalueCutoff; // user input, used to determine score cutoff based on ipwm
int *scoreCutoff; // pwm score cutoff for the corresponding p-value cutoff
double logev; // log of E-value of a motif;
int useChIPscore; // indicator for using ChIP-seq score for seq. selection for EM
int numEM; // number of EM steps
double E_valueCutoff; // log E-value cutoff
//int nsitesEM; // number of binding sites in sequences subjected to EM
int minsitesEM; // minimal number of sites in a motif in EM sequences
int *nsites; // number of binding sites in full data
int minsites; // minimal number of sites in a motif in full data
Sites **site; // binding sites in all sequences
int motifCn; // number of motifs sought and found
int extTrim;
int noMotifFound; // none of the dyads in the population resulted in a motif
char **pwmConsensus; // consensus sequences of motifs
double pwmDistCutoff; // test statistic for motif pwm similarity
char *uniqMotif; // motifs in a population unique or not
int numUniq; // number of unique motifs in a population
int slideWinPWM; // sliding window for comparing pwm similarity
int widthWt; // window width in which nucleotides are given large weights for PWM optimization
int fullScan; // scan scan on the original sequences or masked sequences
// background
int numBackgSets;
// weights
double **posWeight; // spatial weights
int weightType; // four weight types 0, 1, 2, 3, or 4
// words for spaced dyad
Words *word; // top-ranked k-mers as the words for spaced dyads
int numTop3mer,numTop4mer,numTop5mer; // No. of top-ranked k-mers as words for dyads
int maxWordSize; // max of the above three
int numWordGroup; // number of non-zero k-mer groups
int minSpaceWidth,maxSpaceWidth; // min and max width of spacer of the spaced dyads
Chrs **dyad; // initial population of "chromosomes"
char **sdyad; // char of spaced dyads
// GA
int populationSize,numGeneration; // GA parameters
double maxpMutationRate;
Fitness *fitness; // "chromosome" fitness
Wheel *wheel; // roulette-wheel selection
// to speed up only select a subset of sequences for EM algorithm
double fEM; // percentage of sequences used in EM algorithm
int numSeqEM; // number of sequences subject to EM
char *Iseq; // Indicator if a sequence is used in EM or not
int *emSeqLen; // length of sequences used in EM
double *maxpFactor;
int numCycle; // number of GADEM cycles
int generationNoMotif; // maximal number of GA generations in a GADEM cycle resulted in no motifs
// mis.
//seed_t seed; // random seed
int motifCn2,id,numCycleNoMotif,verbose,minminSites,nmotifs;
int startPWMfound,stopCriterion;
char *mFileName,*oFileName,*pwmFileName,*tempRbFileName;
time_t start;
int cn[4],bcn[4],*seqCn,*bseqCn,avebnsites,avebnsiteSeq,totalSitesInput;
int i;
int ii=0;
int jjj=0;
/*************/
FILE * output = fopen("output.txt", "w");
/*************/
GetRNGstate();
mFileName=alloc_char(500); mFileName[0]='\0';
oFileName=alloc_char(500); oFileName[0]='\0';
pwmFileName=alloc_char(500); pwmFileName[0]='\0';
bFileName=alloc_char(500); bFileName[0]='\0';
//tempRbFileName=alloc_char(500); tempRbFileName[0]='\0';
seq=NULL; aveSeqLen=0; maxSeqLen=0;
//minsites=-1;
startPWMfound=0;
maxSeqLen=0;
for(incr=1;incr<longueur;incr=incr+2)
{
if (length(STRING_ELT(sequence,(incr)))>maxSeqLen) maxSeqLen=length(STRING_ELT(sequence,(incr)));
}
// fprintf(output,"maxLength=%d",maxSeqLen);
// exit(0);
seq=alloc_char_char(numSeq,maxSeqLen+1);
for(incr=1;incr<longueur;incr=incr+2)
{
for (int j=0; j<length(STRING_ELT(sequence,(incr))); j++)
{
seq[IncrementTemp][j]=CHAR(STRING_ELT(sequence,(incr)))[j];
}
IncrementTemp++;
}
verbose=LOGICAL_VALUE(Rverbose);
numWordGroup=INTEGER_VALUE(RnumWordGroup);
minsites=INTEGER_VALUE(RminSites);
numTop3mer=INTEGER_VALUE(RnumTop3mer);
numTop4mer=INTEGER_VALUE(RnumTop4mer);
numTop5mer=INTEGER_VALUE(RnumTop5mer);
numGeneration=INTEGER_VALUE(RnumGeneration);
populationSize=INTEGER_VALUE(RpopulationSize);
pvalueCutoff=NUMERIC_VALUE(RpValue);
E_valueCutoff=NUMERIC_VALUE(ReValue);
extTrim=INTEGER_VALUE(RextTrim);
minSpaceWidth=INTEGER_VALUE(RminSpaceWidth);
maxSpaceWidth=INTEGER_VALUE(RmaxSpaceWidth);
useChIPscore=NUMERIC_VALUE(RuseChIPscore);
numEM=INTEGER_VALUE(RnumEM);
fEM=NUMERIC_VALUE(RfEM);
widthWt=INTEGER_VALUE(RwidthWt);
fullScan=INTEGER_VALUE(RfullScan);
slideWinPWM=INTEGER_VALUE(RslideWinPWM);
numUniq=populationSize;
stopCriterion=INTEGER_VALUE(RstopCriterion);
numBackgSets=INTEGER_VALUE(RnumBackgSets);
weightType=NUMERIC_VALUE(RweightType);
//const char *tempRbFileName[1];
tempRbFileName = convertRString2Char(RbFileName);
//tempRbFileName[0]=CHAR(STRING_ELT(RbFileName,0));
nmotifs = INTEGER_VALUE(Rnmotifs);
maskR = INTEGER_VALUE(RmaskR);
if(numSeq>MAX_NUM_SEQ)
{
error("Error: maximal number of seqences reached!\nPlease reset MAX_NUM_SEQ in gadem.h and rebuild (see installation)\n");
}
strcpy(bFileName,tempRbFileName);
ChIPScore=alloc_double(MAX_NUM_SEQ);
seqLen=alloc_int(MAX_NUM_SEQ);
geneID=alloc_int(MAX_NUM_SEQ);
// seq=sequences;
// numSeq=size;
int len;
for (i=0; i<numSeq; i++)
{
len=strlen(seq[i]);
seqLen[i]=len;
geneID[i]=INTEGER(accession)[i];
}
aveSeqLen=0;
for (i=0; i<numSeq; i++) aveSeqLen +=seqLen[i]; aveSeqLen /=(double)numSeq;
for (i=0; i<numSeq; i++) {
if (seqLen[i]>maxSeqLen) maxSeqLen=seqLen[i];
}
rseq=alloc_char_char(numSeq,maxSeqLen+1);
oseq=alloc_char_char(numSeq,maxSeqLen+1);
orseq=alloc_char_char(numSeq,maxSeqLen+1);
for (i=0; i<numSeq; i++)
{
if(seqLen[i]>maxSeqLen) maxSeqLen=seqLen[i];
}
reverse_seq(seq,rseq,numSeq,seqLen);
// make a copy of the original sequences both strands
for (i=0; i<numSeq; i++)
{
for (int j=0; j<seqLen[i]; j++)
{
oseq[i][j]=seq[i][j];
orseq[i][j]=rseq[i][j];
}
oseq[i][seqLen[i]]='\0'; orseq[i][seqLen[i]]='\0';
}
if (strcmp(bFileName,"NULL")!= 0)
{
bfreq0=alloc_double(5);
read_background(bFileName,bfreq0);
}
if (GET_LENGTH(RListPWM)!= 0)
{
startPWMfound=1;
}
else { }
// check for input parameters
if(numGeneration<1)
{
error("number of generaton < 1.\n");
}
if(populationSize<1)
{
error("population size < 1.\n");
}
if (minSpaceWidth<0)
{
error("minimal number of unspecified bases in spaced dyads <0.\n");
}
if (maxSpaceWidth<0)
{
error("maximal number of unspecified bases in spaced dyads <0.\n");
}
if (minSpaceWidth>maxSpaceWidth)
{
error("mingap setting must <= to maxgap setting.\n\n");
}
if (maxSpaceWidth+12>MAX_PWM_LENGTH)
{
error("maxgap setting plus word lengths exceed <MAX_PWM_LENGTH>.\n");
}
if (numEM<0)
{
error("number of EM steps is zero.\n");
}
if (numEM==0)
{
error("number of EM steps = 0, no EM optimization is carried out.\n");
}
if (fullScan!=0 && fullScan!=1)
fullScan=0;
maxWordSize=0;
if (numTop3mer>maxWordSize) maxWordSize=numTop3mer;
if (numTop4mer>maxWordSize) maxWordSize=numTop4mer;
if (numTop5mer>maxWordSize) maxWordSize=numTop5mer;
// any one, two or three: tetramer, pentamer, hexamer
if (numTop3mer==0 && numTop4mer==0 && numTop5mer==0)
{
error("maxw3, maxw4, and maxw5 all zero - no words for spaced dyads.\n");
}
// if (startPWMfound && fEM!=0.5 && fEM!=1.0 & verbose)
// {
// warning("fEM argument is ignored in a seeded analysis\n");
// }
if (startPWMfound)
{
// if(verbose)
// {
// if (populationSize!=10 && populationSize!=100) warning("pop argument is ignored in a seeded analysis, -pop is set to 10.\n");
// if (numGeneration!=1 && numGeneration!=5) warning("gen argument is ignored in a seeded analysis, -gen is set to 1.\n");
// }
fEM=1.0;
populationSize=FIXED_POPULATION; numGeneration=1;
}
// number of sequences for EM
if (fEM>1.0 || fEM<=0.0)
{
error("The fraction of sequences subject to EM is %3.2f.\n",fEM);
}
numSeqEM=(int)(fEM*numSeq);
// memory callocations
Iseq =alloc_char(numSeq+1);
opwm2 =alloc_double_double(MAX_PWM_LENGTH,4);
ipwm =alloc_int_int(MAX_PWM_LENGTH,4);
logepwm=alloc_double_double(MAX_PWM_LENGTH,4);
emSeqLen=alloc_int(numSeqEM);
scoreCutoff=alloc_int(1000);
// scoreCutoff=alloc_int(populationSize);
llrDist=alloc_distr(MAX_DIMENSION);
posWeight=alloc_double_double(numSeq,maxSeqLen);
sseq=alloc_char_char(MAX_NUM_SEQ,maxSeqLen+1);
rsseq=alloc_char_char(MAX_NUM_SEQ,maxSeqLen+1);
bfreq1=base_frequency(numSeq,seq,seqLen);
if (strcmp(bFileName,"NULL") == 0)
{
bfreq0=alloc_double(5);
for (i=0; i<4; i++)
{
bfreq0[i]=bfreq1[i];
}
}
// if minN not specified, set the defaults accordingly
if (minsites==-1)
{
minsites =max(2,(int)(numSeq/20));
}
minsitesEM=(int)(fEM*minsites);
maxpMutationRate=MAXP_MUTATION_RATE;
// determine the distribution and critical cut point
pwmDistCutoff=vector_similarity();
/*---------- select a subset of sequences for EM only --------------*/
if (useChIPscore==1)
{
select_high_scoring_seq_for_EM (ChIPScore,numSeq,numSeqEM,Iseq,fEM);
}
else
{
sample_without_replacement(Iseq,numSeqEM,numSeq);
}
/*-------------------- end of selection --------------------------*/
if (maskR==1) mask_repetitive(geneID,seq,numSeq,seqLen,mFileName);
if (widthWt<20)
{
warning("The window width of sequence centered on the nucleotides having large weights in EM for PWM optimization is small\n Motif longer than %d will not be discovered\n",widthWt);
}
time(&start);
// if (weightType==1 || weightType==3)
//ffprintf(output,fp,"window width of sequence centered on the nucleotides having large weights for PWM optimization: %d\n",widthWt);
//ffprintf(output,fp,"pwm score p-value cutoff for declaring binding site:\t%e\n",pvalueCutoff);
if(verbose)
{
ffprintf(output,output,"==============================================================================================\n");
ffprintf(output,output,"input sequence file: %s\n",mFileName);
fprintf(output,"number of sequences and average length:\t\t\t\t%d %5.1f\n",numSeq,aveSeqLen);
fprintf(output,"Use pgf method to approximate llr null distribution\n");
fprintf(output,"parameters estimated from sequences in: %s\n\n",mFileName);
if (weightType!=0)
fprintf(output,"non-uniform weight applies to each sequence - type:\t\t%d\n",weightType);
fprintf(output,"number of GA generations & population size:\t\t\t%d %d\n\n",numGeneration,populationSize);
fprintf(output,"PWM score p-value cutoff for binding site declaration:\t\t%e\n",pvalueCutoff);
fprintf(output,"ln(E-value) cutoff for motif declaration:\t\t\t%f\n\n",E_valueCutoff);
// fprintf(output,"number (percentage) of sequences selected for EM:\t\t%d(%4.1f\%)\n",numSeqEM,100.0*(double)numSeqEM/(double)numSeq);
fprintf(output,"number of EM steps:\t\t\t\t\t\t%d\n",numEM);
fprintf(output,"minimal no. sites considered for a motif:\t\t\t%d\n\n",minsites);
fprintf(output,"[a,c,g,t] frequencies in input data:\t\t\t\t%f %f %f %f\n",bfreq1[0],bfreq1[1],bfreq1[2],bfreq1[3]);
fprintf(output,"==============================================================================================\n");
}
// if (pgf)
// {
// if (userMarkovOrder!=0 & verbose)
// {
// warning("The user-specified background Markov order (%d) is ignored when -pgf is set to 1\n",userMarkovOrder);
// }
// if (bFileName[0]!='\0' & verbose)
// {
// warning("The user-specified background models: %s are not used when -pgf is set to 1\n",bFileName);
// }
// }
// if (startPWMfound && fEM!=1.0 & verbose)
// {
// warning("fEM argument is ignored in a seeded analysis\n");
// }
// determine seq length by counting only [a,c,g,t], seqLen is used in E-value calculation
// determine the distribution and critical cut point
pwmDistCutoff=vector_similarity();
if (weightType==0) assign_weight_uniform(seqLen,numSeq,posWeight);
else if (weightType==1) assign_weight_triangular(seqLen,numSeq,posWeight);
else if (weightType==2) assign_weight_normal(seqLen,numSeq,posWeight);
else
{
error("Motif prior probability type not found - please choose: 0, 1, or 2\n");
// fprintf(output,"Consider: -posWt 1 for strong central enrichment as in ChIP-seq\n");
// fprintf(output," -posWt 0 for others\n\n");
// exit(0);
}
/* if (startPWMfound) minminSites=minsites;
else minminSites=(int)(0.40*minsitesEM);*/
motifCn=0; noMotifFound=0; numCycle=0; numCycleNoMotif=0;
int compt=0;
int lengthList=GET_LENGTH(RListPWM);
/****************************************/
broadcastOnce(maxSeqLen, numEM, startPWMfound, minminSites, maxpFactor, numSeq, numSeqEM, Iseq, bfreq0, posWeight, weightType, pvalueCutoff, emSeqLen, populationSize);
/****************************************/
do
{
if(!startPWMfound)
{
if(verbose)
{
fprintf(output,"*** Running an unseeded analysis ***\n");
// fprintf(output,"\n|------------------------------------------------------------------|\n");
// fprintf(output,"| |\n");
// fprintf(output,"| *** Running an unseeded analysis *** |\n");
// fprintf(output,"| |\n");
// fprintf(output,"|------------------------------------------------------------------|\n\n");
}
populationSize=INTEGER_VALUE(RpopulationSize);
numGeneration=INTEGER_VALUE(RnumGeneration);
dyad =alloc_chrs(populationSize,4);
wheel =alloc_wheel(populationSize);
fitness=alloc_fitness(populationSize);
maxpFactor=alloc_double(populationSize);
uniqMotif=alloc_char(populationSize+1);
opwm =alloc_double_double_double(populationSize,MAX_PWM_LENGTH,4);
epwm=alloc_double_double_double(populationSize,MAX_PWM_LENGTH,4);
pwmConsensus=alloc_char_char(populationSize,MAX_PWM_LENGTH+1);
pwm =alloc_double_double_double(populationSize,MAX_PWM_LENGTH,4);
pwmLen=alloc_int(populationSize);
sdyad =alloc_char_char(populationSize,MAX_PWM_LENGTH+1);
word =alloc_word(numWordGroup,maxWordSize);
minminSites=(int)(0.40*minsitesEM);
// identify top-ranked k-mers (k=3,4,5) for spaced dyads
if(verbose)
fprintf(output,"GADEM cycle %2d: enumerate and count k-mers... ",numCycle+1);
numWordGroup=word_for_dyad(word,seq,rseq,numSeq,seqLen,bfreq1,&numTop3mer,&numTop4mer,&numTop5mer);
if(verbose)
fprintf(output,"Done.\n");
// generating a "population" of spaced dyads
if(verbose)
fprintf(output,"Initializing GA... ");
initialisation(dyad,populationSize,numWordGroup,word,minSpaceWidth,maxSpaceWidth,maxpFactor);
if(verbose)
fprintf(output,"Done.\n");
}
else
{
if(verbose)
{
fprintf(output,"*** Running an seeded analysis ***\n");
// fprintf(output,"\n|------------------------------------------------------------------|\n");
// fprintf(output,"| |\n");
// fprintf(output,"| *** Running a seeded analysis *** |\n");
// fprintf(output,"| |\n");
// fprintf(output,"|------------------------------------------------------------------|\n\n");
}
populationSize=FIXED_POPULATION;
dyad =alloc_chrs(populationSize,4);
pwm=alloc_double_double_double(populationSize,MAX_PWM_LENGTH,4);
pwmLen=alloc_int(populationSize);
maxpFactor=alloc_double(populationSize);
uniqMotif=alloc_char(populationSize+1);
opwm =alloc_double_double_double(populationSize,MAX_PWM_LENGTH,4);
epwm=alloc_double_double_double(populationSize,MAX_PWM_LENGTH,4);
pwmConsensus=alloc_char_char(populationSize,MAX_PWM_LENGTH+1);
sdyad =alloc_char_char(populationSize,MAX_PWM_LENGTH+1);
word =alloc_word(numWordGroup,maxWordSize);
wheel =alloc_wheel(populationSize);
fitness=alloc_fitness(populationSize);
minminSites=minsites;
int lengthMatrix;
lengthMatrix=GET_LENGTH(VECTOR_ELT(RListPWM,compt));
RSpwm=allocMatrix(REALSXP,4,(lengthMatrix/4));
RSpwm=VECTOR_ELT(RListPWM,compt);
pwmLen[0]=read_pwm0(RSpwm,pwm[0],lengthMatrix);
for(i=1; i<populationSize; i++)
{
for (int j=0; j<pwmLen[0]; j++)
{
for (int k=0; k<4; k++)
{
pwm[i][j][k]=pwm[0][j][k];
}
}
pwmLen[i]=pwmLen[0];
}
for (i=0; i<populationSize; i++)
{
maxpFactor[i]=FIXED_MAXPF*(i+1);
standardize_pwm(pwm[i],pwmLen[i]);
consensus_pwm(pwm[i],pwmLen[i],pwmConsensus[i]);
strcpy(sdyad[i],pwmConsensus[i]);
}
}
generationNoMotif=0;
for (jjj=0; jjj<numGeneration; jjj++)
{
// convert spaced dyads to letter probability matrix
if (!startPWMfound)
{
dyad_to_pwm(word,populationSize,dyad,pwm,pwmLen);
}
/*
DO_APPLY(populationCalculation(maxSeqLen, numEM, fitness+ii,
startPWMfound, minminSites, maxpFactor[ii],
numSeq, numSeqEM, seq, rseq, seqLen, Iseq,
bfreq0, posWeight, weightType,
pvalueCutoff, emSeqLen,
pwm[ii], pwmLen[ii], epwm[ii], opwm[ii],
pwmConsensus[ii], scoreCutoff+ii, sdyad[ii], ii),
populationSize, ii);
*/
/* Create the structure to send to all the other slaves */
broadcastEveryCycle(Iseq, pwm, pwmLen, pwmConsensus, scoreCutoff, sdyad, populationSize);
populationCalculation(maxSeqLen, numEM, fitness+ii,
startPWMfound, minminSites, maxpFactor[ii],
numSeq, numSeqEM, seq, rseq, seqLen, Iseq,
bfreq0, posWeight, weightType,
pvalueCutoff, emSeqLen,
pwm[ii], pwmLen[ii], epwm[ii], opwm[ii],
pwmConsensus[ii], scoreCutoff+ii, sdyad[ii], ii);
/* Receive the analyzed data from all the other slaves and compile them */
//getPopCalcResults(...);
// for (i=0; i<5; i++)
// {
// fprintf(output,"fitness.value=%lf\n",fitness[i].value);
// fprintf(output,"fitness.index=%d\n",fitness[i].index);
// fprintf(output,"maxpfactor=%lf\n",maxpFactor[i]);
// fprintf(output,"scoreCutoff=%d\n",scoreCutoff[i]);
// fprintf(output," spacedDyad: %s\n",sdyad[i]);
//
// for (l=0; l<pwmLen[i]; l++)
// {
// for (m=0; m<4; m++)
// {
// fprintf(output,"opwm[%d][%d][%d]=%lf ",i,l,m,opwm[i][l][m]);
// fprintf(output,"epwm[%d][%d][%d]=%lf ",i,l,m,epwm[i][l][m]);
// fprintf(output,"pwm[%d][%d][%d]=%lf ",i,l,m,pwm[i][l][m]);
// }
// fprintf(output,"\n");
// }
// fprintf(output,"\n");
// }
//
// testrand=runif(0,1);
// fprintf(output,"testrand1=%lf\n",testrand);
if (populationSize>1)
{
sort_fitness(fitness,populationSize);
}
// for (i=0; i<5; i++)
// {
// fprintf(output,"fitness.value=%lf\n",fitness[i].value);
// fprintf(output,"fitness.index=%d\n",fitness[i].index);
// }
numUniq=check_pwm_uniqueness_dist(opwm, pwmLen,
populationSize, fitness,
pwmDistCutoff, E_valueCutoff,
uniqMotif, slideWinPWM);
// for (i=0; i<5; i++)
// {
// fprintf(output,"fitness.value=%lf\n",fitness[i].value);
// fprintf(output,"fitness.index=%d\n",fitness[i].index);
// fprintf(output,"maxpfactor=%lf\n",maxpFactor[i]);
// fprintf(output,"scoreCutoff=%d\n",scoreCutoff[i]);
// fprintf(output," spacedDyad: %s\n",sdyad[i]);
//
// for (l=0; l<pwmLen[i]; l++)
// {
// for (m=0; m<4; m++)
// {
// fprintf(output,"opwm[%d][%d][%d]=%lf",i,l,m,opwm[i][l][m]);
// }
// fprintf(output,"\n");
// }
// fprintf(output,"\n");
// }
if(verbose)
{
fprintf(output,"GADEM cycle[%3d] generation[%3d] number of unique motif: %d\n",numCycle+1,jjj+1,numUniq);
for (i=0; i<populationSize; i++)
{
if (uniqMotif[i]=='1')
{
fprintf(output," spacedDyad: %s ",sdyad[fitness[i].index]);
for (int j=strlen(sdyad[fitness[i].index]); j<maxSpaceWidth+10; j++) fprintf(output," ");
fprintf(output,"motifConsensus: %s ",pwmConsensus[fitness[i].index]);
for (int j=strlen(sdyad[fitness[i].index]); j<maxSpaceWidth+10; j++) fprintf(output," ");
fprintf(output," %3.2f fitness: %7.2f\n",maxpFactor[fitness[i].index],fitness[i].value);
}
}
fprintf(output,"\n");
}
if (jjj<numGeneration-1)
{
// fitness based selection with replacement
roulett_wheel_fitness(fitness,populationSize,wheel);
// mutation and crossover operations
if (populationSize>1)
{
testrand=runif(0,1);
if (testrand>=0.5)
{
mutation(dyad,numWordGroup,word,minSpaceWidth,maxSpaceWidth,wheel,populationSize,fitness,uniqMotif,
maxpFactor,maxpMutationRate);
}
else
{
crossover(dyad,numWordGroup,word,minSpaceWidth,maxSpaceWidth,wheel,populationSize,fitness,uniqMotif, maxpFactor,maxpMutationRate);
}
}
else
{
mutation(dyad,numWordGroup,word,minSpaceWidth,maxSpaceWidth,wheel,populationSize,fitness,uniqMotif, maxpFactor,maxpMutationRate);
}
}
}
if((numCycle+1)< lengthList)
{
compt++;
}
else
{
startPWMfound=0;
}
numCycle++;
site=alloc_site_site(numUniq+1,MAX_SITES);
nsites=alloc_int(numUniq+1);
pwmnewLen=alloc_int(numUniq+1); // after base extension and trimming
seqCn=alloc_int(MAX_NUM_SEQ);
bseqCn=alloc_int(MAX_NUM_SEQ);
// final step user-specified background model is used
motifCn2=0; // motifCn per GADEM cycle
for (ii=0; ii<populationSize; ii++)
{
id=fitness[ii].index;
if(uniqMotif[ii]=='0')
{
continue;
}
// approximate the exact llr distribution using Staden's method
// if(verbose)
// {
// fprintf(output,"Approximate the exact pwm llr score distribution using the pgf method.\n");
// }
log_ratio_to_int(epwm[id],ipwm,pwmLen[id],bfreq0);
// compute score distribution of the (int)PWM using Staden's method
llrDim=pwm_score_dist(ipwm,pwmLen[id],llrDist,bfreq0);
//fprintf(output,"Avant ScoreCutoff %d \n",scoreCutoff[id]);
scoreCutoff[id]=determine_cutoff(llrDist,llrDim,pvalueCutoff);
//fprintf(output,"Apres ScoreCutoff %d \n",scoreCutoff[id]);
if(fullScan)
{
nsites[motifCn2]=scan_llr_pgf(llrDist,llrDim,site[motifCn2],numSeq,oseq,orseq,seqLen,ipwm,pwmLen[id],scoreCutoff[id],bfreq0);
}
else
{
nsites[motifCn2]=scan_llr_pgf(llrDist,llrDim,site[motifCn2],numSeq,seq,rseq,seqLen,ipwm,pwmLen[id],scoreCutoff[id],bfreq0);
}
if (nsites[motifCn2]>=max(2,minsites))
{
for (int j=0; j<numSeq; j++) seqCn[j]=0;
for (int j=0; j<nsites[motifCn2]; j++) seqCn[site[motifCn2][j].seq]++;
for (int j=0; j<4; j++) cn[j]=0;
for (int j=0; j<numSeq; j++)
{
if (seqCn[j]==0) cn[0]++;
if (seqCn[j]==1) cn[1]++;
if (seqCn[j]==2) cn[2]++;
if (seqCn[j]>2) cn[3]++;
}
totalSitesInput=nsites[motifCn2];
if (extTrim)
{
if (fullScan)
{
extend_alignment(site[motifCn2],numSeq,oseq,orseq,seqLen,nsites[motifCn2],pwmLen[id],&(pwmnewLen[motifCn2]));
}
else
{
extend_alignment(site[motifCn2],numSeq,seq,rseq,seqLen,nsites[motifCn2],pwmLen[id],&(pwmnewLen[motifCn2]));
}
}
else
{
pwmnewLen[motifCn2]=pwmLen[id];
}
if (fullScan)
{
align_sites_count(site[motifCn2],oseq,orseq,nsites[motifCn2],pwmnewLen[motifCn2],opwm2);
}
else
{
align_sites_count(site[motifCn2],seq,rseq,nsites[motifCn2],pwmnewLen[motifCn2],opwm2);
}
standardize_pwm(opwm2,pwmnewLen[motifCn2]);
logev=E_value(opwm2,nsites[motifCn2],bfreq0,pwmnewLen[motifCn2],numSeq,seqLen);
if (logev<=E_valueCutoff)
{
consensus_pwm(opwm2,pwmnewLen[motifCn2],pwmConsensus[id]);
if (fullScan)
{
SET_VECTOR_ELT(ResultsGadem,increment,print_result_R(site[motifCn2],nsites[motifCn2],numSeq,oseq,orseq,seqLen,logev,opwm2,pwmnewLen[motifCn2],motifCn+1,sdyad[id],pwmConsensus[id],numCycle,pvalueCutoff,maxpFactor[id],geneID));
increment++;
print_motif(site[motifCn2],nsites[motifCn2],oseq,orseq,seqLen,pwmnewLen[motifCn2],motifCn+1,opwm2);
}
else
{
SET_VECTOR_ELT(ResultsGadem,increment,print_result_R(site[motifCn2],nsites[motifCn2],numSeq,seq,rseq,seqLen,logev,opwm2,pwmnewLen[motifCn2],
motifCn+1,sdyad[id],pwmConsensus[id],numCycle,pvalueCutoff,maxpFactor[id],geneID));
increment++;
print_motif(site[motifCn2],nsites[motifCn2],seq,rseq,seqLen,pwmnewLen[motifCn2],motifCn+1,opwm2);
}
mask_sites(nsites[motifCn2],seq,rseq,seqLen,site[motifCn2],pwmnewLen[motifCn2]);
/* ----------------------compute the average number of sites in background sequences ----------------------*/
avebnsites=0; avebnsiteSeq=0;
for (i=0; i<numBackgSets; i++)
{
simulate_background_seq(bfreq0,numSeq,seqLen,sseq);
reverse_seq(sseq,rsseq,numSeq,seqLen);
nsites[motifCn2]=scan_llr_pgf(llrDist,llrDim,site[motifCn2],numSeq,sseq,rsseq,seqLen,ipwm,pwmLen[id],scoreCutoff[id],bfreq0);
for (int j=0; j<numSeq; j++) bseqCn[j]=0;
for (int j=0; j<nsites[motifCn2]; j++) bseqCn[site[motifCn2][j].seq]++;
for (int j=0; j<4; j++) bcn[j]=0;
for (int j=0; j<numSeq; j++)
{
if (bseqCn[j]==0) bcn[0]++;
if (bseqCn[j]==1) bcn[1]++;
if (bseqCn[j]==2) bcn[2]++;
if (bseqCn[j]>2) bcn[3]++;
}
//ffprintf(output,fq,"background set[%2d] Seqs with 0,1,2,>2 sites: %d %d %d %d\n",i+1,bcn[0],bcn[1],bcn[2],bcn[3]);
avebnsites+=nsites[motifCn2]; avebnsiteSeq+=(numSeq-bcn[0]);
}
avebnsites/=numBackgSets; avebnsiteSeq/=numBackgSets;
/* -----------------end compute the average number of sites in background sequences ----------------------*/
motifCn++; motifCn2++;
//if((numCycle+1) > lengthList & fixSeeded)
// {
// numCycleNoMotif=1;
// startPWMfound=1;
// } else {
numCycleNoMotif=0;
// }
}
}
}
/* for (int i=0; i<motifCn2; i++)
{
mask_sites(nsites[i],seq,rseq,seqLen,site[i],pwmnewLen[i]);
} */
if (site[0])
{
free(site[0]);
site[0]=NULL;
}
if (site)
{
free(site);
site=NULL;
}
if (nsites)
{
free(nsites);
nsites=NULL;
}
if (pwmnewLen)
{
free(pwmnewLen);
pwmnewLen=NULL;
}
if (motifCn2==0)
numCycleNoMotif++;
if (motifCn==nmotifs)
{
fprintf(output,"Maximal number of motifs (%d) reached\n",nmotifs);
break;
}
if (numCycleNoMotif==stopCriterion)
noMotifFound=1;
}while (!noMotifFound);
// fclose(fp);
/*if (!startPWMfound) {
if (dyad[0]) { free(dyad[0]); dyad[0]=NULL; }
if (dyad) { free(dyad); dyad=NULL; }
}*/
if (seqLen)
{
free(seqLen);
seqLen=NULL;
}
if (pwm[0][0])
{
free(pwm[0][0]);
pwm[0][0]=NULL;
}
if (pwm[0])
{
free(pwm[0]);
pwm[0]=NULL;
}
if (pwm)
{
free(pwm);
pwm=NULL;
}
if (opwm2[0])
{
free(opwm2[0]);
opwm2[0]=NULL;
}
if (opwm2)
{
free(opwm2);
opwm2=NULL;
}
if (opwm[0][0])
{
free(opwm[0][0]);
opwm[0][0]=NULL;
}
if (opwm[0])
{
free(opwm[0]);
opwm[0]=NULL;
}
if (opwm)
{
free(opwm);
opwm=NULL;
}
if(ipwm[0])
{
free(ipwm[0]);
ipwm[0]=NULL;
}
if (ipwm)
{
free(ipwm);
ipwm=NULL;
}
if (pwmLen)
{
free(pwmLen);
pwmLen=NULL;
}
if (seq[0]) { free(seq[0]); seq[0]=NULL; }
if (seq) { free(seq); seq=NULL; }
// if (rseq[0]) { free(rseq[0]); rseq[0]=NULL; }
// if (rseq) { free(rseq); rseq=NULL; }
// if (oseq[0]) { free(oseq[0]); oseq[0]=NULL; }
// if (oseq) { free(oseq); oseq=NULL; }
// if (orseq[0]) { free(orseq[0]); orseq[0]=NULL; }
// if (orseq) { free(orseq); orseq=NULL; }
if (bfreq1)
{
free(bfreq1);
bfreq1=NULL;
}
if (bfreq0)
{
free(bfreq0);
bfreq0=NULL;
}
if (wheel)
{
free(wheel);
wheel=NULL;
}
if (fitness)
{
free(fitness);
fitness=NULL;
}
if (mFileName)
{
free(mFileName);
mFileName=NULL;
}
if (oFileName)
{
free(oFileName);
oFileName=NULL;
}
if (pwmFileName)
{
free(pwmFileName);
pwmFileName=NULL;
}
if (sdyad[0])
{
free(sdyad[0]);
sdyad[0]=NULL;
}
if (sdyad)
{
free(sdyad);
sdyad=NULL;
}
if (pwmConsensus[0])
{
free(pwmConsensus[0]);
pwmConsensus[0]=NULL;
}
if (pwmConsensus)
{
free(pwmConsensus);
pwmConsensus=NULL;
}
//if (!startPWMfound && word) destroy_word(word,numWordGroup);
PutRNGstate();
UNPROTECT(1);
return(ResultsGadem);
}