int main(int argc, char **argv)
{
AjPSeqall seqall;
AjPSeqout seqout;
AjPSeq seq = NULL;
AjBool firstonly;
embInit("seqret", argc, argv);
seqout = ajAcdGetSeqoutall("outseq");
seqall = ajAcdGetSeqall("sequence");
firstonly = ajAcdGetBoolean("firstonly");
while(ajSeqallNext(seqall, &seq))
{
ajSeqoutWriteSeq(seqout, seq);
if(firstonly)
break;
}
ajSeqoutClose(seqout);
ajSeqallDel(&seqall);
ajSeqDel(&seq);
ajSeqoutDel(&seqout);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqout seqout;
AjPSeqall seqall;
AjPSeq seq = NULL;
embInit("seqretallfeat", argc, argv);
seqout = ajAcdGetSeqoutall("outseq");
seqall = ajAcdGetSeqall("sequence");
while (ajSeqallNext(seqall, &seq))
{
ajSeqoutWriteSeq(seqout, seq);
ajSeqTrace(seq);
}
ajSeqoutClose(seqout);
ajFeatTest();
ajSeqallDel(&seqall);
ajSeqDel(&seq);
ajSeqoutDel(&seqout);
embExit();
return 0;
}
int main(int argc, char **argv)
{
/* Variable declarations */
AjPFile outf = NULL;
AjPXmlall xmlall = NULL;
AjPXml xml = NULL;
/* ACD processing */
embInit("xmltext", argc, argv);
xmlall = ajAcdGetXmlall("xml");
outf = ajAcdGetOutfile("outfile");
while(ajXmlallNext(xmlall, &xml))
{
ajFmtPrintF(outf,"%S",ajXmlGetEntry(xml));
}
/* Memory clean-up and exit */
ajXmlallDel(&xmlall);
ajXmlDel(&xml);
ajFileClose(&outf);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPFeattaball featin;
AjPFeattable feat = NULL;
embInit("benchmark", argc, argv);
featin = ajAcdGetFeaturesall("features");
long counter = 0;
while(ajFeattaballNext(featin, &feat))
{
counter++;
}
ajFeattableDel(&feat);
ajFeattaballDel(&featin);
printf("Read %li records\n", counter);
embExit();
return 0;
}
int main(int argc, char **argv)
{
/* Variable declarations */
AjPOutfile outfile = NULL;
AjPVarload varload = NULL;
AjPVar variation = NULL;
/* ACD processing */
embInit("variationget", argc, argv);
varload = ajAcdGetVariation("variation");
outfile = ajAcdGetOutvariation("outfile");
while(ajVarloadNext(varload, &variation))
{
while(ajVarloadMore(varload, &variation))
{
ajVaroutWriteNext(outfile, variation);
}
ajOutfileReset(outfile);
}
/* Memory clean-up and exit */
ajVarloadDel(&varload);
ajVarDel(&variation);
ajOutfileClose(&outfile);
embExit();
return 0;
}
int main(int argc, char **argv)
{
/* Variable declarations */
AjPFile outfile = NULL;
AjPResourceall resourceall = NULL;
AjPResource resource = NULL;
/* ACD processing */
embInit("drtext", argc, argv);
resourceall = ajAcdGetResourceall("resources");
outfile = ajAcdGetOutfile("outfile");
while(ajResourceallNext(resourceall, &resource))
{
ajFmtPrintF(outfile,"%S",ajResourceGetEntry(resource));
}
ajResourceallDel(&resourceall);
ajResourceDel(&resource);
ajFileClose(&outfile);
embExit();
return 0;
}
int main(int argc, char **argv)
{
/* Variable declarations */
AjPOutfile outfile = NULL;
AjPResourceall resourceall = NULL;
AjPResource resource = NULL;
/* ACD processing */
embInit("drget", argc, argv);
resourceall = ajAcdGetResourceall("resources");
outfile = ajAcdGetOutresource("outfile");
while(ajResourceallNext(resourceall, &resource))
{
ajResourceoutWrite(outfile, resource);
}
ajResourceallDel(&resourceall);
ajResourceDel(&resource);
ajOutfileClose(&outfile);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqall seqall;
AjPSeq seq = NULL;
AjPFile primerFile; /* read the primer pairs from a file */
AjPFile outf;
AjPList primerList;
ajint mmp = 0;
embInit("primersearch", argc, argv);
seqall = ajAcdGetSeqall("seqall");
outf = ajAcdGetOutfile("outfile");
primerFile = ajAcdGetInfile("infile");
mmp = ajAcdGetInt("mismatchpercent");
/* build list of forward/reverse primer pairs as read from primerfile */
primerList = ajListNew();
/* read in primers from primerfile, classify and compile them */
primersearch_read_primers(&primerList,primerFile, mmp);
/* check there are primers to be searched */
if(!ajListGetLength(primerList))
{
ajErr("No suitable primers found - exiting");
embExitBad();
return 0;
}
/* query sequences one by one */
while(ajSeqallNext(seqall,&seq))
primersearch_primer_search(primerList, seq);
/* output the results */
primersearch_print_hits(primerList, outf);
/* delete all nodes of list, then the list itself */
ajListMap(primerList, primersearch_free_primer, NULL);
ajListFree(&primerList);
ajListFree(&primerList);
ajFileClose(&outf);
ajSeqallDel(&seqall);
ajSeqDel(&seq);
ajFileClose(&primerFile);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqall seqall;
AjPSeqout seqout;
AjPSeqout junkout;
AjPSeq seq = NULL;
AjPStr exclude = NULL;
AjPStr pattern = NULL;
AjPStr name = NULL;
AjPStr acc = NULL;
embInit("notseq", argc, argv);
seqout = ajAcdGetSeqoutall("outseq");
junkout = ajAcdGetSeqoutall("junkoutseq");
seqall = ajAcdGetSeqall("sequence");
exclude = ajAcdGetString("exclude");
notseq_readfile(exclude, &pattern);
while(ajSeqallNext(seqall, &seq))
{
ajStrAssignS(&name, ajSeqGetNameS(seq));
ajStrAssignS(&acc, ajSeqGetAccS(seq));
if(embMiscMatchPatternDelimC(name, pattern, ",;") ||
embMiscMatchPatternDelimC(acc, pattern, ",;"))
ajSeqoutWriteSeq(junkout, seq);
else
/* no match, so not excluded */
ajSeqoutWriteSeq(seqout, seq);
ajStrSetClear(&name);
ajStrSetClear(&acc);
}
ajSeqoutClose(seqout);
ajSeqoutClose(junkout);
ajSeqallDel(&seqall);
ajSeqDel(&seq);
ajSeqoutDel(&seqout);
ajSeqoutDel(&junkout);
ajStrDel(&exclude);
ajStrDel(&pattern);
ajStrDel(&name);
ajStrDel(&acc);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqall seqall;
AjPSeq a;
AjPSeqout outf;
AjPStr substr;
AjPStr back;
AjPStr gctable;
AjPCod codon = NULL;
ajint gctablenum;
ajint beg;
ajint end;
embInit("backtranambig", argc, argv);
seqall = ajAcdGetSeqall("sequence");
outf = ajAcdGetSeqoutall("outfile");
gctable = ajAcdGetListSingle("table");
ajStrToInt(gctable, &gctablenum);
codon = ajCodNewCodenum(gctablenum);
while(ajSeqallNext(seqall, &a))
{
substr = ajStrNew();
beg = ajSeqGetBegin(a);
end = ajSeqGetEnd(a);
ajStrAssignSubC(&substr,ajSeqGetSeqC(a),beg-1,end-1);
back = ajStrNew();
ajCodBacktranslateAmbig(&back,substr,codon);
ajSeqAssignSeqS (a, back);
ajSeqSetNuc(a);
ajSeqoutWriteSeq(outf,a);
}
ajSeqoutClose(outf);
ajStrDel(&back);
ajStrDel(&substr);
ajSeqoutDel(&outf);
ajCodDel(&codon);
ajStrDel(&gctable);
ajSeqallDel(&seqall);
ajSeqDel(&a);
embExit();
return 0;
}
int main(int argc, char **argv)
{
/* Variable Declarations */
AjPFile outf;
AjPStr code = NULL;
char code1;
ajuint i;
ajuint iend;
/* ACD File Processing */
embInit("infobase", argc, argv);
code = ajAcdGetString("code");
outf = ajAcdGetOutfile("outfile");
/* Application logic */
ajStrFmtUpper(&code);
iend = ajStrGetLen(code);
ajFmtPrintF(outf, "%4s %-10s %-10s %s\n",
"Code", "Ambiguity", "Complement", "Mnemonic");
for(i=0;i<iend;i++)
{
code1=ajStrGetCharPos(code,i);
if(ajBaseExistsChar(code1))
{
ajFmtPrintF(outf, "%-4c %-10S %-10c %S\n",
code1, ajBaseGetCodes(code1),
ajBaseAlphacharComp(code1),
ajBaseGetMnemonic(code1));
}
else
{
ajFmtPrintF(outf, "%-4c %-10s %-10c %s\n",
code1, ".",
'.',
"invalid");
}
}
/* Memory management and exit */
ajStrDel(&code);
ajFileClose(&outf);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqset seqset;
AjPSeqall seqall;
AjPSeq seq;
ajint i = 0;
AjPStr kimout = NULL;
AjPStr dir = NULL;
AjPFile obofile = NULL;
AjPFile resfile = NULL;
AjPDir taxdir = NULL;
embInit("ajtest", argc, argv);
seqall = ajAcdGetSeqall ("sequence");
seqset = ajAcdGetSeqset ("bsequence");
dir = ajAcdGetOutdirName("outdir");
obofile = ajAcdGetInfile ("obofile");
taxdir = ajAcdGetDirectory ("taxdir");
resfile = ajAcdGetInfile ("dbxreffile");
ajUser("Directory '%S'", dir);
ajUser("Set of %d", ajSeqsetGetSize(seqset));
while(ajSeqallNext (seqall, &seq))
{
ajUser ("%3d <%S>", i++, ajSeqGetUsaS(seq));
ajFmtPrintS(&kimout, "kim%d.out", i);
ajtest_kim (kimout, seq);
}
ajSeqDel(&seq);
ajSeqallDel(&seqall);
ajSeqsetDel(&seqset);
ajStrDel(&kimout);
ajStrDel(&dir);
if(taxdir)
ajTaxLoad(taxdir);
ajDirDel(&taxdir);
if(obofile)
ajOboParseObofile(obofile, "");
ajFileClose(&obofile);
if(resfile)
ajResourceParse(resfile, "");
ajFileClose(&resfile);
embExit();
return 0;
}
int main(int argc, char **argv)
{
/* Variable Declarations */
AjPFile inf = NULL;
AjPFile outf = NULL;
AjPStr line = NULL; /* Line from inf */
AjPStr option = NULL;
AjBool doall = AJFALSE;
AjBool doend = AJFALSE;
AjBool doexcess = AJFALSE;
/* ACD File Processing */
embInit("nospace", argc, argv);
inf = ajAcdGetInfile("infile");
outf = ajAcdGetOutfile("outfile");
option = ajAcdGetListSingle("menu");
if(ajStrMatchC(option, "all"))
doall = ajTrue;
else if(ajStrMatchC(option, "end"))
doend = ajTrue;
else if(ajStrMatchC(option, "excess"))
doexcess = ajTrue;
/* Application logic */
line = ajStrNew();
while(ajReadline(inf,&line))
{
if(doall)
ajStrRemoveWhite(&line);
else if(doend)
ajStrTrimWhiteEnd(&line);
else if(doexcess)
ajStrRemoveWhiteExcess(&line);
ajFmtPrintF(outf, "%S\n", line);
}
/* Memory management and exit */
ajFileClose(&inf);
ajFileClose(&outf);
ajStrDel(&line);
ajStrDel(&option);
embExit();
return 0;
}
int main(int argc, char **argv)
{
if(argc < 2)
ajFatal("Error - must specify an application to compile\n");
ajAcdSetControl("acdtrace");
ajAcdSetControl("acdnocommandline");
embInit(argv[1], argc-1, &argv[1]);
ajAcdExit(ajTrue); /* turn off the 'never used' ACD warnings */
embExit();
return 0;
}
int main(int argc, char **argv)
{
/* Variable Declarations */
AjPSeqset seqset = NULL;
AjPAlign align = NULL;
AjPStr name = NULL;
AjPStr comment = NULL;
AjBool append = ajFalse;
AjPStr header = NULL;
/* ACD File Processing */
embInit("aligncopy", argc, argv);
seqset = ajAcdGetSeqset("sequences");
align = ajAcdGetAlign("outfile");
name = ajAcdGetString("name");
comment = ajAcdGetString("comment");
append = ajAcdGetBoolean("append");
/* Application logic */
ajAlignDefine(align, seqset);
if(ajStrGetLen(name))
ajFmtPrintS(&header, "Alignment: %S\n\n", name);
ajStrAppendS(&header, comment);
if(append)
ajAlignSetHeaderApp(align, header);
else
ajAlignSetHeader(align, header);
ajAlignWrite(align);
ajAlignClose(align);
/* Memory management and exit */
ajSeqsetDel(&seqset);
ajAlignDel(&align);
ajStrDel(&name);
ajStrDel(&comment);
ajStrDel(&header);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqall seqall;
AjPSeq seq;
AjPFile outf;
AjPCod codon;
AjPStr substr;
ajint beg;
ajint end;
ajint ccnt;
embInit("cusp", argc, argv);
seqall = ajAcdGetSeqall("sequence");
outf = ajAcdGetOutfile("outfile");
ccnt = 0;
substr = ajStrNew();
codon = ajCodNewCodenum(0);
ajCodSetNameS(codon, ajFileGetPrintnameS(outf));
while(ajSeqallNext(seqall, &seq))
{
beg = ajSeqallGetseqBegin(seqall);
end = ajSeqallGetseqEnd(seqall);
ajStrAssignSubS(&substr,ajSeqGetSeqS(seq),beg-1,end-1);
ajCodSetTripletsS(codon,substr,&ccnt);
}
ajCodCalcUsage(codon,ccnt);
ajCodSetDescC(codon, "CUSP codon usage file");
ajCodWrite(codon, outf);
ajFileClose(&outf);
ajStrDel(&substr);
ajCodDel(&codon);
ajSeqallDel(&seqall);
ajSeqDel(&seq);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqall seqall;
AjPSeq seq;
AjPTable table = 0;
AjPFile outf;
ajint wordsize;
ajint mincount;
embInit("wordcount", argc, argv);
seqall = ajAcdGetSeqall("sequence1");
wordsize = ajAcdGetInt("wordsize");
outf = ajAcdGetOutfile("outfile");
mincount = ajAcdGetInt("mincount");
embWordLength(wordsize);
while (ajSeqallNext(seqall, &seq))
{
embWordGetTable(&table, seq); /* get table of words */
}
embWordPrintTableFI(table, mincount, outf); /* print table of words */
/*
** test if table can be added to
** if(getWordTable(&table, seq, wordcount)) ?? get table of words ??
** {
** printWordTable(table); ?? print table of words ??
** }
*/
embWordFreeTable(&table); /* free table of words */
ajSeqallDel(&seqall);
ajSeqDel(&seq);
ajFileClose(&outf);
embExit();
return 0;
}
int main(int argc, char **argv)
{
/* Variable Declarations */
AjPSeqset seqset = NULL;
AjPSeqout seqout = NULL;
AjBool bigfirst;
ajuint nseqs;
ajuint i;
/* ACD File Processing */
embInit("sizeseq", argc, argv);
seqset = ajAcdGetSeqset("sequences");
bigfirst = ajAcdGetBoolean("descending");
seqout = ajAcdGetSeqoutall("outseq");
/* Application logic */
ajSeqsetSortLen(seqset);
nseqs = ajSeqsetGetSize(seqset);
if(bigfirst)
{
for(i=nseqs; i>0; i--)
ajSeqoutWriteSeq(seqout, ajSeqsetGetseqSeq(seqset,i-1));
}
else
{
for(i=0; i<nseqs; i++)
ajSeqoutWriteSeq(seqout, ajSeqsetGetseqSeq(seqset,i));
}
/* Memory management and exit */
ajSeqsetDel(&seqset);
ajSeqoutClose(seqout);
ajSeqoutDel(&seqout);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqall seqall;
AjPFile outf;
AjPSeq seq = NULL;
AjPList xrefs = NULL;
ajuint nrefs;
AjPSeqXref xref = NULL;
embInit("seqxref", argc, argv);
outf = ajAcdGetOutfile("outfile");
seqall = ajAcdGetSeqall("sequence");
xrefs = ajListNew();
while(ajSeqallNext(seqall, &seq))
{
nrefs = ajSeqGetXrefs(seq, xrefs);
ajSeqxreflistSort(xrefs);
ajFmtPrintF(outf, "#%S: %u\n", ajSeqGetUsaS(seq), nrefs);
while(ajListPop(xrefs, (void**)&xref))
{
ajFmtPrintF(outf, "%S:%S\n", xref->Db, xref->Id);
ajSeqxrefDel(&xref);
}
}
ajListFree(&xrefs);
ajSeqDel(&seq);
ajFileClose(&outf);
ajSeqallDel(&seqall);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPStr directory = NULL;
AjPTable mtable = NULL;
AjPList jdirlist = NULL;
AjPStr jdirloc = NULL;
embInit("jaspextract",argc,argv);
directory = ajAcdGetDirectoryName("directory");
mtable = ajTablestrNewLen(PFMNUMGUESS);
jdirlist = ajListNew();
jaspextract_openoutdirs();
jaspextract_copyfiles(directory);
jaspextract_readmatrixlist(mtable, directory);
jaspextract_getjaspdirs(jdirlist);
while(ajListPop(jdirlist,(void **)&jdirloc))
{
jaspextract_writematrixfile(mtable, jdirloc);
ajStrDel(&jdirloc);
}
ajTablestrFree(&mtable);
ajStrDel(&directory);
ajListFree(&jdirlist);
embExit();
return 0;
}
int getorf_acd(int argc, char **argv) {
AjPSeqout seqout;
AjPSeqall seqall;
AjPStr tablestr;
ajuint minsize;
ajuint maxsize;
AjPStr findstr;
AjBool methionine;
AjBool circular;
AjBool reverse;
ajint around;
embInit("getorf", argc, argv);
seqout = ajAcdGetSeqoutall("outseq");
seqall = ajAcdGetSeqall("sequence");
tablestr = ajAcdGetListSingle("table");
minsize = ajAcdGetInt("minsize");
maxsize = ajAcdGetInt("maxsize");
findstr = ajAcdGetListSingle("find");
methionine = ajAcdGetBoolean("methionine");
circular = ajAcdGetBoolean("circular");
reverse = ajAcdGetBoolean("reverse");
around = ajAcdGetInt("flanking");
getorf(seqout, seqall, tablestr, minsize, maxsize, findstr, methionine, circular, reverse, around);
ajSeqoutClose(seqout);
ajSeqallDel(&seqall);
ajSeqoutDel(&seqout);
ajStrDel(&tablestr);
ajStrDel(&findstr);
embExit();
return 0;
}
int main(int argc, char **argv)
{
/* Variable declarations */
AjPOutfile outfile = NULL;
AjPRefseq refseq = NULL;
/* ACD processing */
embInit("refseqget", argc, argv);
refseq = ajAcdGetRefseq("refsequence");
outfile = ajAcdGetOutrefseq("outfile");
ajRefseqoutWrite(outfile, refseq);
/* Memory clean-up and exit */
ajRefseqDel(&refseq);
ajOutfileClose(&outfile);
embExit();
return 0;
}
int main(int argc, char **argv)
{
/* Variable Declarations */
AjPSeqset seqset = NULL;
AjPMatrixf fmat = NULL;
float thresh;
float threshlow;
float threshup;
float gapopen;
float gapextend;
AjPSeqout seqout = NULL;
AjPSeqout seqoutred = NULL;
AjPStr mode = NULL;
ajint moden;
ajuint i;
/* toggle "feature" from ACD not retrieved ... no need */
const AjPSeq seq = NULL;
AjPList list = NULL; /* List for redundancy removal. */
AjPUint keep = NULL; /* 1: Sequence in list was non-redundant,
0: redundant. */
ajuint nseq = 0; /* No. seqs. in list. */
ajint nseqnr = 0; /* No. non-redundant seqs. in list. */
/* ACD File Processing */
embInit("skipredundant", argc, argv);
seqset = ajAcdGetSeqset("sequences");
mode = ajAcdGetListSingle("mode");
fmat = ajAcdGetMatrixf("datafile");
thresh = ajAcdGetFloat("threshold");
threshlow = ajAcdGetFloat("minthreshold");
threshup = ajAcdGetFloat("maxthreshold");
gapopen = ajAcdGetFloat("gapopen");
gapextend = ajAcdGetFloat("gapextend");
seqout = ajAcdGetSeqoutall("outseq");
seqoutred = ajAcdGetSeqoutall("redundantoutseq");
/* Application logic */
list = ajListNew();
skipredundant_SeqsetToList(list, seqset);
keep = ajUintNew();
ajStrToInt(mode, &moden);
if(moden == 1)
/* Remove redundancy at a single threshold % sequence similarity */
{
if((!embDmxSeqNR(list, &keep, &nseqnr, fmat, gapopen,
gapextend, thresh, ajFalse)))
ajFatal("embDmxSeqNR unexpected failure!");
}
else if (moden == 2)
/* 2: Remove redundancy outside a range of acceptable threshold % similarity */
{
if((!embDmxSeqNRRange(list, &keep, &nseqnr, fmat, gapopen,
gapextend, threshlow, threshup, ajFalse)))
ajFatal("embDmxSeqNRRange unexpected failure!");
}
else
ajFatal("Invalid mode (not 1 or 2) which should never occur (check ACD file!)");
nseq = ajSeqsetGetSize(seqset);
for(i=0; i<nseq; i++)
{
seq = ajSeqsetGetseqSeq(seqset, i);
if(ajUintGet(keep, i))
ajSeqoutWriteSeq(seqout, seq);
else if(seqoutred)
ajSeqoutWriteSeq(seqoutred, seq);
}
/* Memory management and exit */
ajSeqsetDel(&seqset);
ajMatrixfDel(&fmat);
ajStrDel(&mode);
ajSeqoutClose(seqout);
ajSeqoutDel(&seqout);
if(seqoutred)
{
ajSeqoutClose(seqoutred);
ajSeqoutDel(&seqoutred);
}
skipredundant_ClearList(list);
ajListFree(&list);
ajUintDel(&keep);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqall seqall;
AjPSeq seq = NULL;
AjPReport report = NULL;
AjPStr jaspdir = NULL;
AjPStr menu = NULL;
AjPStr substr = NULL;
AjPStr mats = NULL;
AjPStr excl = NULL;
float thresh = 0.;
ajuint recurs = 0;
AjPStr dir = NULL;
AjPStr mfname = NULL;
AjPList flist = NULL;
AjPList hits = NULL;
AjPStr head = NULL;
ajint begin;
ajint end;
ajuint mno;
char cp;
ajuint i;
AjPTable mattab = NULL;
AjPFeattable TabRpt = NULL;
AjBool both = ajFalse;
embInit("jaspscan", argc, argv);
seqall = ajAcdGetSeqall("sequence");
menu = ajAcdGetListSingle("menu");
mats = ajAcdGetString("matrices");
excl = ajAcdGetString("exclude");
thresh = ajAcdGetFloat("threshold");
report = ajAcdGetReport("outfile");
both = ajAcdGetBoolean("both");
jaspdir = ajStrNew();
substr = ajStrNew();
flist = ajListNew();
hits = ajListNew();
dir = ajStrNew();
head = ajStrNew();
cp = ajStrGetCharFirst(menu);
if(cp=='C')
ajStrAssignC(&jaspdir,J_COR);
else if(cp=='F')
ajStrAssignC(&jaspdir,J_FAM);
else if(cp=='P')
ajStrAssignC(&jaspdir,J_PHY);
else if(cp=='N')
ajStrAssignC(&jaspdir,J_CNE);
else if(cp=='O')
ajStrAssignC(&jaspdir,J_POL);
else if(cp=='S')
ajStrAssignC(&jaspdir,J_SPL);
else
ajFatal("Invalid JASPAR database selection");
ajStrAssignS(&dir, ajDatafileValuePath());
if(!ajStrGetLen(dir))
ajFatal("EMBOSS DATA directory couldn't be determined");
jaspscan_ParseInput(dir, jaspdir, mats, excl, &recurs, flist);
mno = ajListGetLength(flist);
if(cp == 'C')
mattab = jaspscan_ReadCoreList(jaspdir);
if(cp == 'F')
mattab = jaspscan_ReadFamList(jaspdir);
if(cp == 'P')
mattab = jaspscan_ReadCoreList(jaspdir);
if(cp == 'N')
mattab = jaspscan_ReadCoreList(jaspdir);
if(cp == 'O')
mattab = jaspscan_ReadCoreList(jaspdir);
if(cp == 'S')
mattab = jaspscan_ReadCoreList(jaspdir);
ajFmtPrintS(&head,"Database scanned: %S Threshold: %.3f",jaspdir,thresh);
ajReportSetHeaderS(report,head);
while(ajSeqallNext(seqall, &seq))
{
begin = ajSeqallGetseqBegin(seqall);
end = ajSeqallGetseqEnd(seqall);
ajStrAssignSubC(&substr,ajSeqGetSeqC(seq),begin-1,end-1);
ajStrFmtUpper(&substr);
TabRpt = ajFeattableNewSeq(seq);
for(i=0; i < mno; ++i)
{
ajListPop(flist,(void **)&mfname);
jaspscan_scan(substr,begin,mfname, cp, thresh, both, hits);
ajListPushAppend(flist, (void **)mfname);
}
jaspscan_ReportHits(TabRpt,mattab,hits);
ajReportWrite(report, TabRpt, seq);
ajFeattableDel(&TabRpt);
}
while(ajListPop(flist,(void **)&mfname))
ajStrDel(&mfname);
ajStrDel(&dir);
ajStrDel(&menu);
ajStrDel(&excl);
ajStrDel(&substr);
ajStrDel(&mats);
ajStrDel(&head);
ajStrDel(&jaspdir);
ajSeqDel(&seq);
ajTableMapDel(mattab,jaspscan_ClearTable,NULL);
ajTableFree(&mattab);
ajListFree(&flist);
ajListFree(&hits);
ajSeqallDel(&seqall);
ajReportDel(&report);
embExit();
return 0;
}
int main(int argc, char **argv)
{
ajint begin, end;
AjPSeqall seqall;
AjPSeq seq;
EmbPShow ss;
AjPFile outfile;
AjPStr tablename;
ajint table;
AjPRange uppercase;
AjPRange highlight;
AjBool threeletter;
AjBool numberseq;
AjBool nameseq;
ajint width;
ajint length;
ajint margin;
AjBool description;
ajint offset;
AjBool html;
AjPStr descriptionline;
ajint orfminsize;
AjPTrn trnTable;
AjBool translation;
AjBool reverse;
AjBool cutlist;
AjBool flat;
EmbPMatMatch mm = NULL;
AjPStr *framelist;
AjBool frames[6]; /* frames to be translated 1 to 3, -1 to -3 */
/* stuff for tables and lists of enzymes and hits */
ajint default_mincuts = 1;
ajint default_maxcuts = 2000000000;
AjPTable hittable; /* enzyme hits */
/* stuff lifted from Alan's 'restrict.c' */
AjPStr enzymes = NULL;
ajint mincuts;
ajint maxcuts;
ajint sitelen;
AjBool single;
AjBool blunt;
AjBool sticky;
AjBool ambiguity;
AjBool plasmid;
AjBool commercial;
AjBool limit;
AjBool methyl;
AjPFile enzfile = NULL;
AjPFile equfile = NULL;
AjPFile methfile = NULL;
AjPTable retable = NULL;
ajint hits;
AjPList restrictlist = NULL;
embInit("remap", argc, argv);
seqall = ajAcdGetSeqall("sequence");
outfile = ajAcdGetOutfile("outfile");
tablename = ajAcdGetListSingle("table");
uppercase = ajAcdGetRange("uppercase");
highlight = ajAcdGetRange("highlight");
threeletter = ajAcdGetBoolean("threeletter");
numberseq = ajAcdGetBoolean("number");
width = ajAcdGetInt("width");
length = ajAcdGetInt("length");
margin = ajAcdGetInt("margin");
nameseq = ajAcdGetBoolean("name");
description = ajAcdGetBoolean("description");
offset = ajAcdGetInt("offset");
html = ajAcdGetBoolean("html");
orfminsize = ajAcdGetInt("orfminsize");
translation = ajAcdGetBoolean("translation");
reverse = ajAcdGetBoolean("reverse");
cutlist = ajAcdGetBoolean("cutlist");
flat = ajAcdGetBoolean("flatreformat");
framelist = ajAcdGetList("frame");
/* restriction enzyme stuff */
mincuts = ajAcdGetInt("mincuts");
maxcuts = ajAcdGetInt("maxcuts");
sitelen = ajAcdGetInt("sitelen");
single = ajAcdGetBoolean("single");
blunt = ajAcdGetBoolean("blunt");
sticky = ajAcdGetBoolean("sticky");
ambiguity = ajAcdGetBoolean("ambiguity");
plasmid = ajAcdGetBoolean("plasmid");
commercial = ajAcdGetBoolean("commercial");
limit = ajAcdGetBoolean("limit");
enzymes = ajAcdGetString("enzymes");
methfile = ajAcdGetDatafile("mfile");
methyl = ajAcdGetBoolean("methylation");
if(!blunt && !sticky)
ajFatal("Blunt/Sticky end cutters shouldn't both be disabled.");
/* get the number of the genetic code used */
ajStrToInt(tablename, &table);
trnTable = ajTrnNewI(table);
/* read the local file of enzymes names */
remap_read_file_of_enzyme_names(&enzymes);
/* get the frames to be translated */
remap_GetFrames(framelist, frames);
while(ajSeqallNext(seqall, &seq))
{
/* get begin and end positions */
begin = ajSeqGetBegin(seq)-1;
end = ajSeqGetEnd(seq)-1;
/* do the name and description */
if(nameseq)
{
if(html)
ajFmtPrintF(outfile, "<H2>%S</H2>\n",
ajSeqGetNameS(seq));
else
ajFmtPrintF(outfile, "%S\n", ajSeqGetNameS(seq));
}
if(description)
{
/*
** wrap the description line at the width of the sequence
** plus margin
*/
if(html)
ajFmtPrintF(outfile, "<H3>%S</H3>\n",
ajSeqGetDescS(seq));
else
{
descriptionline = ajStrNew();
ajStrAssignS(&descriptionline, ajSeqGetDescS(seq));
ajStrFmtWrap(&descriptionline, width+margin);
ajFmtPrintF(outfile, "%S\n", descriptionline);
ajStrDel(&descriptionline);
}
}
/* get the restriction cut sites */
/*
** most of this is lifted from the program 'restrict.c' by Alan
** Bleasby
*/
if(single)
maxcuts=mincuts=1;
retable = ajTablestrNew(EQUGUESS);
enzfile = ajDatafileNewInNameC(ENZDATA);
if(!enzfile)
ajFatal("Cannot locate enzyme file. Run REBASEEXTRACT");
if(limit)
{
equfile = ajDatafileNewInNameC(EQUDATA);
if(!equfile)
limit = ajFalse;
else
remap_read_equiv(&equfile, &retable, commercial);
}
ajFileSeek(enzfile, 0L, 0);
restrictlist = ajListNew();
/* search for hits, but don't use mincuts and maxcuts criteria yet */
hits = embPatRestrictMatch(seq, begin+1, end+1, enzfile, methfile,
enzymes, sitelen,plasmid, ambiguity,
default_mincuts, default_maxcuts, blunt,
sticky, commercial, methyl,
restrictlist);
ajDebug("Remap found %d hits\n", hits);
if(hits)
{
/* this bit is lifted from printHits */
embPatRestrictRestrict(restrictlist, hits, !limit,
ajFalse);
if(limit)
remap_RestrictPreferred(restrictlist,retable);
}
ajFileClose(&enzfile);
ajFileClose(&methfile);
/*
** Remove those violating the mincuts and maxcuts
** criteria, but save them in hittable for printing out later.
** Keep a count of how many hits each enzyme gets in hittable.
*/
hittable = ajTablestrNewCase(TABLEGUESS);
remap_RemoveMinMax(restrictlist, hittable, mincuts, maxcuts);
/* make the Show Object */
ss = embShowNew(seq, begin, end, width, length, margin, html, offset);
if(html)
ajFmtPrintF(outfile, "<PRE>");
/* create the format to display */
embShowAddBlank(ss);
embShowAddRE(ss, 1, restrictlist, plasmid, flat);
embShowAddSeq(ss, numberseq, threeletter, uppercase, highlight);
if(!numberseq)
embShowAddTicknum(ss);
embShowAddTicks(ss);
if(reverse)
{
embShowAddComp(ss, numberseq);
embShowAddRE(ss, -1, restrictlist, plasmid, flat);
}
if(translation)
{
if(reverse)
embShowAddBlank(ss);
if(frames[0])
embShowAddTran(ss, trnTable, 1, threeletter,
numberseq, NULL, orfminsize,
AJFALSE, AJFALSE, AJFALSE, AJFALSE);
if(frames[1])
embShowAddTran(ss, trnTable, 2, threeletter,
numberseq, NULL, orfminsize,
AJFALSE, AJFALSE, AJFALSE, AJFALSE);
if(frames[2])
embShowAddTran(ss, trnTable, 3, threeletter,
numberseq, NULL, orfminsize,
AJFALSE, AJFALSE, AJFALSE, AJFALSE);
if(reverse)
{
embShowAddTicks(ss);
if(frames[5])
embShowAddTran(ss, trnTable, -3, threeletter,
numberseq, NULL, orfminsize,
AJFALSE, AJFALSE, AJFALSE, AJFALSE);
if(frames[4])
embShowAddTran(ss, trnTable, -2, threeletter,
numberseq, NULL, orfminsize,
AJFALSE, AJFALSE, AJFALSE, AJFALSE);
if(frames[3])
embShowAddTran(ss, trnTable, -1, threeletter,
numberseq, NULL, orfminsize,
AJFALSE, AJFALSE, AJFALSE, AJFALSE);
}
}
embShowPrint(outfile, ss);
/* display a list of the Enzymes that cut and don't cut */
if(cutlist)
{
remap_CutList(outfile, hittable,
limit, html, mincuts, maxcuts);
remap_NoCutList(outfile, hittable, html, enzymes, blunt,
sticky, sitelen, commercial, ambiguity,
limit, retable);
}
/* add a gratuitous newline at the end of the sequence */
ajFmtPrintF(outfile, "\n");
/* tidy up */
embShowDel(&ss);
while(ajListPop(restrictlist,(void **)&mm))
embMatMatchDel(&mm);
ajListFree(&restrictlist);
remap_DelTable(&hittable);
ajTablestrFree(&retable);
}
ajTrnDel(&trnTable);
ajSeqallDel(&seqall);
ajSeqDel(&seq);
ajFileClose(&outfile);
ajStrDel(&tablename);
ajStrDel(&enzymes);
ajStrDelarray(&framelist);
ajRangeDel(&uppercase);
ajRangeDel(&highlight);
embExit();
return 0;
}
int main(int argc, char **argv)
{
/* Variable Declarations */
AjPStr code = NULL;
AjPFile mfptr = NULL;
AjPFile wfptr = NULL;
AjPFile outf = NULL;
EmbPPropMolwt *mwdata;
EmbPPropAmino *aadata;
float *dhstat = NULL;
char code1;
AjPStr code3 = NULL;
ajint idx = 0;
ajuint i;
ajuint iend;
AjPStr propstr = NULL;
float charge;
char csign;
/* ACD File Processing */
embInit("inforesidue", argc, argv);
code = ajAcdGetString("code");
mfptr = ajAcdGetDatafile("aadata");
wfptr = ajAcdGetDatafile("mwdata");
outf = ajAcdGetOutfile("outfile");
aadata = embPropEaminoRead(mfptr);
mwdata = embPropEmolwtRead(wfptr);
if(!embReadAminoDataFloatC(DAYHOFF_FILE,&dhstat,(float)0.001))
ajFatal("Set the EMBOSS_DATA environment variable");
/* Application logic */
ajStrFmtUpper(&code);
iend = ajStrGetLen(code);
ajFmtPrintF(outf, "%-4s %-5s %-20s %6s %9s %-30s %s\n",
"Code", "Short", "Mnemonic",
"Charge", "MolWt",
"Properties", "Ambiguity");
for(i=0; i<iend; i++)
{
code1=ajStrGetCharPos(code,i);
if(ajResidueExistsChar(code1))
{
idx = ajBasecodeToInt(code1);
ajResidueToTriplet(code1, &code3);
ajStrFmtTitle(&code3);
if(!embPropGetProperties(aadata[idx], &propstr))
ajStrAssignC(&propstr, "(none)");
charge = embPropGetCharge(aadata[idx]);
if(charge > 0.0)
csign = '+';
else if(charge < 0.0)
csign = '-';
else
csign = ' ';
ajFmtPrintF(outf, "%-4c %-5S %-20S %3c%3.1f %9.4f %-30S %S\n",
code1, code3, ajResidueGetMnemonic(code1),
csign, fabs(charge),
embPropMolwtGetMolwt(mwdata[idx]),
propstr, ajResidueGetCodes(code1));
}
else
{
ajFmtPrintF(outf, "%-4c %-5s %-20s %6s %9s %-30s %s\n",
code1, ".", "invalid", ".", ".", ".", ".");
}
}
/* Memory management and exit */
ajStrDel(&code);
ajStrDel(&code3);
ajStrDel(&propstr);
ajFileClose(&outf);
ajFileClose(&mfptr);
ajFileClose(&wfptr);
ajFileClose(&outf);
embPropAminoDel(&aadata);
embPropMolwtDel(&mwdata);
AJFREE(dhstat);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqall seqall;
AjPSeqout seqout;
AjPSeq seq;
ajint size;
ajint overlap;
ajint len;
ajint pos;
AjBool addover;
AjBool feature;
AjPStr outseq_name = ajStrNew();
ajint start;
ajint end;
embInit("splitter", argc, argv);
seqout = ajAcdGetSeqoutall("outseq");
seqall = ajAcdGetSeqall("sequence");
size = ajAcdGetInt("size");
overlap = ajAcdGetInt("overlap");
addover = ajAcdGetBoolean("addoverlap");
feature = ajAcdGetBoolean("feature");
while(ajSeqallNext(seqall, &seq))
{
ajSeqTrim(seq);
len = ajSeqGetLen(seq);
pos = 0;
ajStrAssignC(&outseq_name, "");
if (!addover)
{
while(pos+size <= len-1)
{
start = pos;
end = pos+size-1;
splitter_MakeSubSeqName (&outseq_name, seq, start, end);
splitter_ProcessChunk (seqout, seq, start, end,
outseq_name, feature);
pos += size-overlap;
}
}
else
{
while(pos+size+overlap < len-1)
{
start = pos;
end = pos+size+overlap-1;
splitter_MakeSubSeqName (&outseq_name, seq, start, end);
splitter_ProcessChunk (seqout, seq, start, end,
outseq_name, feature);
pos += size;
}
}
splitter_MakeSubSeqName(&outseq_name, seq, pos, len-1);
splitter_ProcessChunk (seqout, seq, pos, len-1,
outseq_name, feature);
}
ajSeqoutClose(seqout);
ajSeqallDel(&seqall);
ajSeqoutDel(&seqout);
ajSeqDel(&seq);
ajStrDel(&outseq_name);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPFile infile = NULL;
AjPFile outfile = NULL;
AjPStr line;
AjPGraph graphLB = NULL;
AjPGraphdata xygraph = NULL;
AjPGraphdata xygraph2 = NULL;
AjBool doplot;
ajint N=0;
float *xdata = NULL;
float *ydata = NULL;
float *V = NULL;
float *S = NULL;
float a;
float b;
float upperXlimit;
float upperYlimit;
float A;
float B;
float C;
float D;
float xmin;
float xmax;
float ymin;
float ymax;
float xmin2;
float xmax2;
float ymin2;
float ymax2;
float Vmax;
float Km;
float cutx;
float cuty;
float amin = 0.;
float amax = 0.;
float bmin = 0.;
float bmax = 0.;
embInit("findkm", argc, argv);
infile = ajAcdGetInfile("infile");
outfile = ajAcdGetOutfile ("outfile");
doplot = ajAcdGetBoolean("plot");
graphLB = ajAcdGetGraphxy("graphLB");
line = ajStrNew();
/* Determine N by reading infile */
while(ajReadlineTrim(infile, &line))
if(ajStrGetLen(line) >0)
N++;
/* only allocate memory to the arrays */
AJCNEW(xdata, N);
AJCNEW(ydata, N);
AJCNEW(S, N);
AJCNEW(V, N);
ajFileSeek(infile, 0L, 0);
N=0;
while(ajReadlineTrim(infile, &line))
{
if(ajStrGetLen(line) > 0)
{
sscanf(ajStrGetPtr(line),"%f %f",&S[N],&V[N]);
if(S[N] > 0.0 && V[N] > 0.0)
{
xdata[N] = S[N];
ydata[N] = S[N]/V[N];
N++;
}
}
}
/* find the max and min values for the graph parameters*/
xmin = (float)0.5*findkm_findmin(xdata, N);
xmax = (float)1.5*findkm_findmax(xdata, N);
ymin = (float)0.5*findkm_findmin(ydata, N);
ymax = (float)1.5*findkm_findmax(ydata, N);
xmin2 = (float)0.5*findkm_findmin(S, N);
xmax2 = (float)1.5*findkm_findmax(S, N);
ymin2 = (float)0.5*findkm_findmin(V, N);
ymax2 = (float)1.5*findkm_findmax(V, N);
/*
** In case the casted ints turn out to be same number on the axis,
** make the max number larger than the min so graph can be seen.
*/
if((ajint)xmax == (ajint)xmin)
++xmax;
if((ajint)ymax == (ajint)ymin)
++ymax;
if((ajint)xmax2 == (ajint)xmin2)
++xmax2;
if((ajint)ymax2 == (ajint)ymin2)
++ymax2;
/*
** Gaussian Elimination for Best-fit curve plotting and
** calculating Km and Vmax
*/
A = findkm_summation(xdata, N);
B = findkm_summation(ydata, N);
C = findkm_multisum(xdata, ydata, N);
D = findkm_multisum(xdata, xdata, N);
/*
** To find the best fit line, Least Squares Fit: y =ax +b;
** Two Simultaneous equations, REARRANGE FOR b
**
** findkm_summation(ydata, N) - findkm_summation(xdata,N)*a - N*b =0;
** b = (findkm_summation(ydata,N) - findkm_summation(xdata,N)*a) / N;
** b = (B - A*a)/ N;
**
** C - D*a - A*((B - A*a)/ N) =0;
** C - D*a - A*B/N + A*A*a/N =0;
** C - A*B/N = D*a - A*A*a/N;
*/
/* REARRANGE FOR a */
a = (N*C - A*B)/ (N*D - A*A);
b = (B - A*a)/ N;
/*
** Equation of Line - Lineweaver burk eqn
** 1/V = (Km/Vmax)*(1/S) + 1/Vmax;
*/
Vmax = 1/a;
Km = b/a;
cutx = -1/Km;
cuty = Km/Vmax;
/* set limits for last point on graph */
upperXlimit = findkm_findmax(xdata,N)+3;
upperYlimit = (upperXlimit)*a + b;
ajFmtPrintF(outfile, "---Hanes Woolf Plot Calculations---\n");
ajFmtPrintF(outfile, "Slope of best fit line is a = %.2f\n", a);
ajFmtPrintF(outfile,"Coefficient in Eqn of line y = ma +b is b "
"= %.2f\n", b);
ajFmtPrintF(outfile, "Where line cuts x axis = (%.2f, 0)\n", cutx);
ajFmtPrintF(outfile, "Where line cuts y axis = (0, %.2f)\n", cuty);
ajFmtPrintF(outfile, "Limit-point of graph for plot = (%.2f, %.2f)\n\n",
upperXlimit, upperYlimit);
ajFmtPrintF(outfile, "Vmax = %.2f, Km = %f\n",Vmax, Km);
/* draw graphs */
if(doplot)
{
xygraph = ajGraphdataNewI(N);
ajGraphdataAddXY(xygraph, S, V);
ajGraphDataAdd(graphLB, xygraph);
ajGraphdataSetTitleC(xygraph, "Michaelis Menten Plot");
ajGraphdataSetXlabelC(xygraph, "[S]");
ajGraphdataSetYlabelC(xygraph, "V");
ajGraphxySetXstartF(graphLB, 0.0);
ajGraphxySetXendF(graphLB, xmax2);
ajGraphxySetYstartF(graphLB, 0.0);
ajGraphxySetYendF(graphLB, ymax2);
ajGraphxySetXrangeII(graphLB, (ajint)0.0, (ajint)xmax2);
ajGraphxySetYrangeII(graphLB, (ajint)0.0, (ajint)ymax2);
ajGraphdataAddposLine(xygraph, 0.0, 0.0, S[0], V[0], (ajint)BLACK);
ajGraphxyShowPointsCircle(graphLB, ajTrue);
ajGraphdataSetMinmax(xygraph,0.0,xmax2,0.0,ymax2);
ajGraphicsCalcRange(S,N,&amin,&amax);
ajGraphicsCalcRange(V,N,&bmin,&bmax);
ajGraphdataSetTruescale(xygraph,amin,amax,bmin,bmax);
ajGraphdataSetTypeC(xygraph,"2D Plot Float");
xygraph2 = ajGraphdataNewI(N);
ajGraphdataAddXY(xygraph2, xdata, ydata);
ajGraphDataAdd(graphLB, xygraph2);
ajGraphdataSetTitleC(xygraph2, "Hanes Woolf Plot");
ajGraphdataSetXlabelC(xygraph2, "[S]");
ajGraphdataSetYlabelC(xygraph2, "[S]/V");
ajGraphxySetXstartF(graphLB, cutx);
ajGraphxySetXendF(graphLB, upperXlimit);
ajGraphxySetYstartF(graphLB, 0.0);
ajGraphxySetYendF(graphLB, upperYlimit);
ajGraphxySetXrangeII(graphLB, (ajint)cutx, (ajint)upperXlimit);
ajGraphxySetYrangeII(graphLB, (ajint)0.0, (ajint)upperYlimit);
ajGraphxyShowPointsCircle(graphLB, ajTrue);
ajGraphdataSetMinmax(xygraph2, cutx,upperXlimit,0.0,upperYlimit);
ajGraphicsCalcRange(xdata,N,&amin,&amax);
ajGraphicsCalcRange(ydata,N,&bmin,&bmax);
ajGraphdataSetTruescale(xygraph2,amin,amax,bmin,bmax);
ajGraphdataSetTypeC(xygraph2,"2D Plot");
ajGraphSetTitleC(graphLB,"FindKm");
ajGraphxySetflagOverlay(graphLB,ajFalse);
ajGraphxyDisplay(graphLB, ajTrue);
}
AJFREE(xdata);
AJFREE(ydata);
AJFREE(S);
AJFREE(V);
ajFileClose(&infile);
ajFileClose(&outfile);
ajGraphxyDel(&graphLB);
ajStrDel(&line);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqset seq1;
AjPSeqset seq2;
AjPFile list;
ajint n1;
ajint n2;
ajint *lengths1;
ajint *lengths2;
ajuint *order1;
ajuint *order2;
ajint *hits1;
ajint *hits2;
ajint curr1;
ajint curr2;
ajint tmp1;
ajint tmp2 = 0;
ajint i;
AjPStr operator;
ajint OperatorCode=0;
embInit("listor", argc, argv);
seq1 = ajAcdGetSeqset("firstsequences");
seq2 = ajAcdGetSeqset("secondsequences");
list = ajAcdGetOutfile("outfile");
operator = ajAcdGetListSingle("operator");
/* get the operator value */
switch(ajStrGetCharFirst(operator))
{
case 'O':
OperatorCode = L_OR;
break;
case 'A':
OperatorCode = L_AND;
break;
case 'X':
OperatorCode = L_XOR;
break;
case 'N':
OperatorCode = L_NOT;
break;
default:
ajFatal("Invalid operator type: %S", operator);
embExitBad();
}
/* get the order of seqset 1 by length */
n1 = ajSeqsetGetSize(seq1);
/* lengths of seq1 entries */
lengths1 = AJCALLOC0(n1, sizeof(ajint));
/* seq1 entries which match seq2 */
hits1 = AJCALLOC0(n1, sizeof(ajint));
/* seq1 entries in length order */
order1 = AJCALLOC0(n1, sizeof(ajint));
for(i=0; i<n1; i++)
{
lengths1[i] = ajSeqGetLen(ajSeqsetGetseqSeq(seq1, i));
order1[i] = i;
hits1[i] = -1;
}
ajSortIntIncI(lengths1, order1, n1);
/* get the order of seqset 2 by length */
n2 = ajSeqsetGetSize(seq2);
lengths2 = AJCALLOC0(n2, sizeof(ajint));
hits2 = AJCALLOC0(n2, sizeof(ajint));
order2 = AJCALLOC0(n2, sizeof(ajint));
for(i=0; i<n2; i++)
{
lengths2[i] = ajSeqGetLen(ajSeqsetGetseqSeq(seq2, i));
order2[i] = i;
hits2[i] = -1;
}
ajSortIntIncI(lengths2, order2, n2);
/*
** go down the two sequence sets, by size order, looking for identical
**lengths
*/
curr1 = 0;
curr2 = 0;
while(curr1 < n1 && curr2 < n2)
{
if(lengths1[order1[curr1]] < lengths2[order2[curr2]])
/* seq1 is shorter - increment curr1 index */
curr1++;
else if(lengths1[order1[curr1]] > lengths2[order2[curr2]])
/* seq2 is shorter - increment curr2 index */
curr2++;
else
{
/* identical lengths - check all seq1/seq2 entries of this len */
for(tmp1=curr1; tmp1<n1
&& lengths1[order1[tmp1]] == lengths2[order2[curr2]]; tmp1++)
for(tmp2=curr2; tmp2<n2 && lengths2[order2[tmp2]] ==
lengths2[order2[curr2]]; tmp2++)
/* check to see if the sequences are identical */
if(!ajStrCmpCaseS(ajSeqGetSeqS(ajSeqsetGetseqSeq(seq1,
order1[tmp1])),
ajSeqGetSeqS(ajSeqsetGetseqSeq(seq2,
order2[tmp2]))))
{
hits1[order1[tmp1]] = order2[tmp2];
hits2[order2[tmp2]] = order1[tmp1];
}
curr1 = tmp1;
curr2 = tmp2;
}
}
/* output the required entries to the list file */
listor_Output(list, OperatorCode, seq1, seq2, hits1, hits2, n1, n2);
AJFREE(lengths1);
AJFREE(lengths2);
AJFREE(order1);
AJFREE(order2);
AJFREE(hits1);
AJFREE(hits2);
ajFileClose(&list);
ajStrDel(&operator);
ajSeqsetDel(&seq1);
ajSeqsetDel(&seq2);
embExit();
return 0;
}
int main(int argc, char **argv)
{
AjPSeqall seqall;
AjPSeq seq = NULL;
AjPFile outf = NULL;
AjPStr strand = NULL;
AjPStr substr = NULL;
AjPStr bases = NULL;
ajint begin;
ajint end;
ajint len;
ajint minlen;
float minobsexp;
float minpc;
ajint window;
ajint shift;
ajint plotstart;
ajint plotend;
float *xypc = NULL;
float *obsexp = NULL;
AjBool *thresh = NULL;
float obsexpmax;
ajint i;
ajint maxarr;
embInit("newcpgreport",argc,argv);
seqall = ajAcdGetSeqall("sequence");
window = ajAcdGetInt("window");
shift = ajAcdGetInt("shift");
outf = ajAcdGetOutfile("outfile");
minobsexp = ajAcdGetFloat("minoe");
minlen = ajAcdGetInt("minlen");
minpc = ajAcdGetFloat("minpc");
substr = ajStrNew();
bases = ajStrNewC("CG");
maxarr = 0;
while(ajSeqallNext(seqall, &seq))
{
begin = ajSeqallGetseqBegin(seqall);
end = ajSeqallGetseqEnd(seqall);
strand = ajSeqGetSeqCopyS(seq);
ajStrFmtUpper(&strand);
ajStrAssignSubC(&substr,ajStrGetPtr(strand),--begin,--end);
len=ajStrGetLen(substr);
if(len > maxarr)
{
AJCRESIZE(obsexp, len);
AJCRESIZE(thresh, len);
AJCRESIZE(xypc, len);
maxarr = len;
}
for(i=0;i<len;++i)
obsexp[i]=xypc[i]=0.0;
newcpgreport_findbases(substr, len, window, shift, obsexp,
xypc, bases, &obsexpmax, &plotstart, &plotend);
newcpgreport_identify(outf, obsexp, xypc, thresh, 0, len, shift,
ajStrGetPtr(bases), ajSeqGetNameC(seq), minlen,
minobsexp, minpc, ajStrGetPtr(strand));
ajStrDel(&strand);
}
ajStrDel(&bases);
ajSeqDel(&seq);
ajStrDel(&substr);
ajFileClose(&outf);
AJFREE(obsexp);
AJFREE(thresh);
AJFREE(xypc);
ajSeqallDel(&seqall);
embExit();
return 0;
}