static int gt_encseq_info_runner(GT_UNUSED int argc, const char **argv,
int parsed_args, void *tool_arguments,
GtError *err)
{
GtEncseqInfoArguments *arguments = tool_arguments;
int had_err = 0;
GtAlphabet *alpha;
const GtUchar *chars;
gt_error_check(err);
gt_assert(arguments);
if (arguments->nomap) {
GtEncseqMetadata *emd = gt_encseq_metadata_new(argv[parsed_args], err);
if (!emd)
had_err = -1;
if (!had_err) {
if (!arguments->noindexname) {
gt_file_xprintf(arguments->outfp, "index name: ");
gt_file_xprintf(arguments->outfp, "%s\n", argv[parsed_args]);
}
gt_file_xprintf(arguments->outfp, "file format version: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"\n",
gt_encseq_metadata_version(emd));
gt_file_xprintf(arguments->outfp, "64-bit file: ");
gt_file_xprintf(arguments->outfp, "%s\n", gt_encseq_metadata_is64bit(emd)
? "yes"
: "no");
gt_file_xprintf(arguments->outfp, "total length: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"\n",
gt_encseq_metadata_total_length(emd));
gt_file_xprintf(arguments->outfp, "number of sequences: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"\n",
gt_encseq_metadata_num_of_sequences(emd));
gt_file_xprintf(arguments->outfp, "number of files: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"\n",
gt_encseq_metadata_num_of_files(emd));
gt_file_xprintf(arguments->outfp, "length of shortest/longest "
"sequence: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"/"GT_WU"\n",
gt_encseq_metadata_min_seq_length(emd),
gt_encseq_metadata_max_seq_length(emd));
gt_file_xprintf(arguments->outfp, "accesstype: ");
gt_file_xprintf(arguments->outfp, "%s\n",
gt_encseq_access_type_str(gt_encseq_metadata_accesstype(emd)));
alpha = gt_encseq_metadata_alphabet(emd);
chars = gt_alphabet_characters(alpha);
gt_file_xprintf(arguments->outfp, "alphabet size: ");
gt_file_xprintf(arguments->outfp, "%u\n",
gt_alphabet_num_of_chars(alpha));
gt_file_xprintf(arguments->outfp, "alphabet characters: ");
gt_file_xprintf(arguments->outfp, "%.*s", gt_alphabet_num_of_chars(alpha),
(char*) chars);
if (gt_alphabet_is_dna(alpha))
gt_file_xprintf(arguments->outfp, " (DNA)");
if (gt_alphabet_is_protein(alpha))
gt_file_xprintf(arguments->outfp, " (Protein)");
gt_file_xprintf(arguments->outfp, "\n");
if (arguments->show_alphabet) {
GtStr *out = gt_str_new();
gt_alphabet_to_str(alpha, out);
gt_file_xprintf(arguments->outfp, "alphabet definition:\n");
gt_file_xprintf(arguments->outfp, "%s\n", gt_str_get(out));
gt_str_delete(out);
}
}
gt_encseq_metadata_delete(emd);
} else {
GtEncseqLoader *encseq_loader;
GtEncseq *encseq;
encseq_loader = gt_encseq_loader_new();
if (arguments->mirror)
gt_encseq_loader_mirror(encseq_loader);
if (!(encseq = gt_encseq_loader_load(encseq_loader,
argv[parsed_args], err)))
had_err = -1;
if (!had_err) {
const GtStrArray *filenames;
GtUword i;
if (!arguments->noindexname) {
gt_file_xprintf(arguments->outfp, "index name: ");
gt_file_xprintf(arguments->outfp, "%s\n", argv[parsed_args]);
}
gt_file_xprintf(arguments->outfp, "file format version: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"\n", gt_encseq_version(encseq));
gt_file_xprintf(arguments->outfp, "64-bit file: ");
gt_file_xprintf(arguments->outfp, "%s\n", gt_encseq_is_64_bit(encseq)
? "yes"
: "no");
gt_file_xprintf(arguments->outfp, "total length: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"\n",
gt_encseq_total_length(encseq));
gt_file_xprintf(arguments->outfp, "compressed size: ");
gt_file_xprintf(arguments->outfp, ""GT_WU" bytes\n",
gt_encseq_sizeofrep(encseq));
gt_file_xprintf(arguments->outfp, "number of sequences: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"\n",
gt_encseq_num_of_sequences(encseq));
gt_file_xprintf(arguments->outfp, "number of files: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"\n",
gt_encseq_num_of_files(encseq));
gt_file_xprintf(arguments->outfp, "length of shortest/longest "
"sequence: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"/"GT_WU"\n",
gt_encseq_min_seq_length(encseq),
gt_encseq_max_seq_length(encseq));
filenames = gt_encseq_filenames(encseq);
gt_file_xprintf(arguments->outfp, "original filenames:\n");
for (i = 0; i < gt_str_array_size(filenames); i++) {
gt_file_xprintf(arguments->outfp, "\t%s ("GT_WU" characters)\n",
gt_str_array_get(filenames, i),
(GtUword)
gt_encseq_effective_filelength(encseq, i));
}
alpha = gt_encseq_alphabet(encseq);
chars = gt_alphabet_characters(alpha);
gt_file_xprintf(arguments->outfp, "alphabet size: ");
gt_file_xprintf(arguments->outfp, "%u\n",
gt_alphabet_num_of_chars(alpha));
gt_file_xprintf(arguments->outfp, "alphabet characters: ");
gt_file_xprintf(arguments->outfp, "%.*s", gt_alphabet_num_of_chars(alpha),
(char*) chars);
if (gt_alphabet_is_dna(alpha))
gt_file_xprintf(arguments->outfp, " (DNA)");
if (gt_alphabet_is_protein(alpha))
gt_file_xprintf(arguments->outfp, " (Protein)");
gt_file_xprintf(arguments->outfp, "\n");
if (arguments->show_alphabet) {
GtStr *out = gt_str_new();
gt_alphabet_to_str(alpha, out);
gt_file_xprintf(arguments->outfp, "alphabet definition:\n");
gt_file_xprintf(arguments->outfp, "%s\n", gt_str_get(out));
gt_str_delete(out);
}
gt_file_xprintf(arguments->outfp, "character distribution:\n");
for (i = 0; i < gt_alphabet_num_of_chars(alpha); i++) {
GtUword cc;
cc = gt_encseq_charcount(encseq, gt_alphabet_encode(alpha, chars[i]));
gt_file_xprintf(arguments->outfp, "\t%c: "GT_WU" (%.2f%%)\n",
(char) chars[i],
cc,
(cc /(double) (gt_encseq_total_length(encseq)
- gt_encseq_num_of_sequences(encseq)+1))*100);
}
gt_file_xprintf(arguments->outfp, "number of wildcards: ");
gt_file_xprintf(arguments->outfp, ""GT_WU" ("GT_WU" range(s))\n",
gt_encseq_wildcards(encseq),
gt_encseq_realwildcardranges(encseq));
gt_file_xprintf(arguments->outfp, "number of special characters: ");
gt_file_xprintf(arguments->outfp, ""GT_WU" ("GT_WU" range(s))\n",
gt_encseq_specialcharacters(encseq),
gt_encseq_realspecialranges(encseq));
gt_file_xprintf(arguments->outfp, "length of longest non-special "
"character stretch: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"\n",
gt_encseq_lengthoflongestnonspecial(encseq));
gt_file_xprintf(arguments->outfp, "accesstype: ");
gt_file_xprintf(arguments->outfp, "%s\n",
gt_encseq_access_type_str(gt_encseq_accesstype_get(encseq)));
gt_file_xprintf(arguments->outfp, "bits used per character: ");
gt_file_xprintf(arguments->outfp, "%f\n",
(double) ((uint64_t) CHAR_BIT *
(uint64_t) gt_encseq_sizeofrep(encseq)) /
(double) gt_encseq_total_length(encseq));
gt_file_xprintf(arguments->outfp, "has special ranges: ");
gt_file_xprintf(arguments->outfp, "%s\n",
gt_encseq_has_specialranges(encseq)
? "yes"
: "no");
gt_file_xprintf(arguments->outfp, "has description support: ");
gt_file_xprintf(arguments->outfp, "%s\n",
gt_encseq_has_description_support(encseq)
? "yes"
: "no");
if (gt_encseq_has_description_support(encseq)) {
gt_file_xprintf(arguments->outfp, "length of longest description: ");
gt_file_xprintf(arguments->outfp, ""GT_WU"\n",
gt_encseq_max_desc_length(encseq));
}
gt_file_xprintf(arguments->outfp, "has multiple sequence support: ");
gt_file_xprintf(arguments->outfp, "%s\n",
gt_encseq_has_multiseq_support(encseq)
? "yes"
: "no");
}
gt_encseq_delete(encseq);
gt_encseq_loader_delete(encseq_loader);
}
return had_err;
}
static int gt_readjoiner_assembly_runner(GT_UNUSED int argc,
GT_UNUSED const char **argv, GT_UNUSED int parsed_args,
void *tool_arguments, GtError *err)
{
GtReadjoinerAssemblyArguments *arguments = tool_arguments;
GtLogger *verbose_logger, *default_logger;
GtEncseqLoader *el;
GtEncseq *reads;
GtTimer *timer = NULL;
GtStrgraph *strgraph = NULL;
GtBitsequence *contained = NULL;
const char *readset = gt_str_get(arguments->readset);
bool eqlen = true;
GtUword nreads, tlen, rlen;
int had_err = 0;
gt_assert(arguments);
gt_error_check(err);
default_logger = gt_logger_new(!arguments->quiet, GT_LOGGER_DEFLT_PREFIX,
stdout);
gt_logger_log(default_logger,
"gt readjoiner assembly (version "GT_READJOINER_VERSION")");
verbose_logger = gt_logger_new(arguments->verbose, GT_LOGGER_DEFLT_PREFIX,
stdout);
gt_logger_log(verbose_logger, "verbose output activated");
gt_logger_log(verbose_logger, "readset name = %s", readset);
if (gt_showtime_enabled())
{
timer = gt_timer_new_with_progress_description(
GT_READJOINER_ASSEMBLY_MSG_COUNTSPM);
gt_timer_start(timer);
gt_timer_show_cpu_time_by_progress(timer);
}
if (!arguments->paths2seq)
{
el = gt_encseq_loader_new();
gt_encseq_loader_drop_description_support(el);
gt_encseq_loader_disable_autosupport(el);
reads = gt_encseq_loader_load(el, readset, err);
if (reads == NULL)
{
had_err = -1;
}
if (had_err == 0)
{
eqlen = gt_encseq_accesstype_get(reads) == GT_ACCESS_TYPE_EQUALLENGTH;
nreads = gt_encseq_num_of_sequences(reads);
gt_logger_log(default_logger, "number of reads in filtered readset = "
GT_WU, nreads);
tlen = gt_encseq_total_length(reads) - nreads + 1;
gt_logger_log(verbose_logger, "total length of filtered readset = " GT_WU,
tlen);
if (eqlen)
{
rlen = gt_encseq_seqlength(reads, 0);
gt_logger_log(verbose_logger, "read length = " GT_WU, rlen);
gt_encseq_delete(reads);
reads = NULL;
}
else
{
had_err = gt_readjoiner_assembly_build_contained_reads_list(
arguments, &contained, err);
rlen = 0;
gt_logger_log(verbose_logger, "read length = variable");
gt_assert(reads != NULL);
}
}
if (had_err == 0)
{
if (!arguments->load)
{
had_err = gt_readjoiner_assembly_build_graph(arguments, &strgraph,
reads, readset, eqlen, rlen, nreads, contained, default_logger,
verbose_logger, timer, err);
}
else
{
gt_readjoiner_assembly_load_graph(&strgraph, reads, readset, rlen,
default_logger, timer);
}
}
if (!eqlen && reads != NULL && !arguments->errors)
{
gt_encseq_delete(reads);
reads = NULL;
if (had_err == 0)
gt_strgraph_set_encseq(strgraph, NULL);
}
if (had_err == 0 && arguments->redtrans)
{
if (gt_showtime_enabled())
gt_timer_show_progress(timer, GT_READJOINER_ASSEMBLY_MSG_REDTRANS,
stdout);
gt_strgraph_sort_edges_by_len(strgraph, false);
(void)gt_strgraph_redtrans(strgraph, false);
(void)gt_strgraph_redself(strgraph, false);
(void)gt_strgraph_redwithrc(strgraph, false);
gt_strgraph_log_stats(strgraph, verbose_logger);
}
if (had_err == 0 && arguments->errors)
{
if (gt_showtime_enabled())
gt_timer_show_progress(timer, GT_READJOINER_ASSEMBLY_MSG_CLEANSG,
stdout);
gt_logger_log(default_logger, GT_READJOINER_ASSEMBLY_MSG_CLEANSG);
had_err = gt_readjoiner_assembly_error_correction(strgraph,
arguments->bubble, arguments->deadend, arguments->deadend_depth,
verbose_logger);
}
if (had_err == 0 && arguments->save)
{
if (gt_showtime_enabled())
gt_timer_show_progress(timer, GT_READJOINER_ASSEMBLY_MSG_SAVESG,
stdout);
gt_logger_log(default_logger, GT_READJOINER_ASSEMBLY_MSG_SAVESG);
gt_strgraph_show(strgraph, GT_STRGRAPH_BIN,
gt_str_get(arguments->readset), GT_READJOINER_SUFFIX_SG, false);
}
if (!eqlen && reads != NULL)
{
gt_encseq_delete(reads);
reads = NULL;
if (had_err == 0)
gt_strgraph_set_encseq(strgraph, NULL);
}
if (had_err == 0)
{
if (gt_showtime_enabled())
gt_timer_show_progress(timer, GT_READJOINER_ASSEMBLY_MSG_TRAVERSESG,
stdout);
gt_logger_log(default_logger, GT_READJOINER_ASSEMBLY_MSG_TRAVERSESG);
gt_readjoiner_assembly_show_current_space("(before traversal)");
gt_strgraph_spell(strgraph, (GtUword)arguments->depthcutoff,
(GtUword)arguments->lengthcutoff, arguments->vd, readset,
GT_READJOINER_SUFFIX_CONTIG_PATHS, NULL, true,
arguments->show_contigs_info, false, verbose_logger);
}
if (contained != NULL)
gt_free(contained);
gt_strgraph_delete(strgraph);
strgraph = NULL;
gt_assert(reads == NULL);
gt_encseq_loader_delete(el);
}
if (had_err == 0)
{
gt_readjoiner_assembly_show_current_space("(before paths2seq)");
had_err = gt_readjoiner_assembly_paths2seq(readset,
(GtUword)arguments->lengthcutoff, arguments->vd,
arguments->astat, arguments->coverage, arguments->copynum,
arguments->buffersize, default_logger, &timer, err);
}
if (gt_showtime_enabled())
{
gt_timer_show_progress_final(timer, stdout);
gt_timer_delete(timer);
}
gt_logger_delete(default_logger);
gt_logger_delete(verbose_logger);
return had_err;
}
static int gt_readjoiner_cnttest_runner(GT_UNUSED int argc,
GT_UNUSED const char **argv, GT_UNUSED int parsed_args,
void *tool_arguments, GT_UNUSED GtError *err)
{
GtReadjoinerCnttestArguments *arguments = tool_arguments;
GtEncseqLoader *el = NULL;
GtEncseq *reads = NULL;
GtBitsequence *bits = NULL;
GtUword nofreads;
int had_err = 0;
gt_error_check(err);
gt_assert(arguments);
if (arguments->test == GT_READJOINER_CNTTEST_SHOWLIST)
{
GtStr *fn = NULL;
fn = gt_str_clone(arguments->readset);
gt_str_append_cstr(fn, GT_READJOINER_SUFFIX_CNTLIST);
had_err = gt_cntlist_parse(gt_str_get(fn), true, &bits, &nofreads, err);
gt_str_delete(fn);
}
else if (arguments->test == GT_READJOINER_CNTTEST_BRUTEFORCE ||
arguments->test == GT_READJOINER_CNTTEST_KMP)
{
el = gt_encseq_loader_new();
gt_encseq_loader_drop_description_support(el);
gt_encseq_loader_disable_autosupport(el);
if (!arguments->singlestrand)
gt_encseq_loader_mirror(el);
reads = gt_encseq_loader_load(el, gt_str_get(arguments->readset), err);
if (reads == NULL)
had_err = -1;
else
{
gt_rdj_pairwise_exact(GT_OVLFIND_CNT, reads, !arguments->singlestrand,
false, arguments->test == GT_READJOINER_CNTTEST_KMP, 1UL, true,
NULL, NULL, false, NULL, &bits, &nofreads);
}
gt_encseq_delete(reads);
gt_encseq_loader_delete(el);
}
else if (arguments->test == GT_READJOINER_CNTTEST_ESA)
{
Sequentialsuffixarrayreader *ssar = NULL;
GtUword readlength = 0, firstrevcompl = 0;
GtLogger *verbose_logger = gt_logger_new(arguments->verbose,
GT_LOGGER_DEFLT_PREFIX, stderr);
ssar = gt_newSequentialsuffixarrayreaderfromfile(gt_str_get(
arguments->readset), SARR_LCPTAB | SARR_SUFTAB | SARR_SSPTAB,
true, verbose_logger, err);
if (gt_error_is_set(err))
had_err = -1;
else
{
nofreads = gt_encseq_num_of_sequences(ssar->encseq);
if (!arguments->singlestrand)
{
nofreads = GT_DIV2(nofreads);
firstrevcompl = nofreads;
}
GT_INITBITTAB(bits, nofreads);
if (!arguments->singlestrand)
if (gt_encseq_accesstype_get(ssar->encseq) == GT_ACCESS_TYPE_EQUALLENGTH)
readlength = gt_encseq_seqlength(ssar->encseq, 0);
(void)gt_contfind_bottomup(ssar, false, bits, arguments->singlestrand ? 0
: firstrevcompl, readlength);
}
if (ssar != NULL)
gt_freeSequentialsuffixarrayreader(&ssar);
gt_logger_delete(verbose_logger);
}
else
{
gt_assert(false);
}
if (!had_err)
had_err = gt_cntlist_show(bits, nofreads, NULL, false, err);
gt_free(bits);
return had_err;
}
