int main(int argc,char **argv)
{
int i;
AlignGeneModelParam * agmp;
GeneStats * gs;
GeneModelParam * gmp = NULL;
CompProb * comp_prob;
DnaProbMatrix * dm;
CodonTable * ct;
RandomModel * rm;
Sequence * test;
ct = read_CodonTable_file("codon.table");
rm = default_RandomModel();
comp_prob = read_Blast_file_CompProb("wag85");
gmp = new_GeneModelParam_from_argv(&argc,argv);
dm = DnaProbMatrix_from_match(0.8,NMaskType_VARIABLE);
if((gs=GeneStats_from_GeneModelParam(gmp)) == NULL ) {
fatal("Could not build gene stats");
}
agmp = std_AlignGeneModelParam(comp_prob,dm,ct,gs);
test = read_fasta_file_Sequence(argv[1]);
assert(test);
for(i=0;i<test->len;i++) {
fprintf(stdout,"%c ss5 %.6f ss3 %.6f\n",test->seq[i],prob_SpliceSiteProb(agmp->ss5,test,i),prob_SpliceSiteProb(agmp->ss3,test,i));
}
}
int main(int argc,char ** argv)
{
int i;
char * temp;
build_defaults();
strip_out_standard_options(&argc,argv,show_help,show_version);
potential_file = strip_out_assigned_argument(&argc,argv,"pg");
pal_file = strip_out_assigned_argument(&argc,argv,"pal_file");
if( (temp = strip_out_assigned_argument(&argc,argv,"gap")) != NULL )
gap_str = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"g")) != NULL )
gap_str = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"ext")) != NULL )
ext_str = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"e")) != NULL )
ext_str = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"matrix")) != NULL )
matrix_file = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"m")) != NULL )
matrix_file = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"s")) != NULL )
qstart_str = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"t")) != NULL )
qend_str = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"u")) != NULL )
tstart_str = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"v")) != NULL )
tend_str = temp;
if( (strip_out_boolean_argument(&argc,argv,"trev")) == TRUE )
reverse = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"[no]newgene")) == TRUE )
use_new_stats = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"tfor")) == TRUE ){
if( reverse == TRUE ) {
warn("You have specified both trev and tfor. Treating as both");
do_both = TRUE;
reverse = FALSE;
} else {
reverse = FALSE;
}
}
if( (temp = strip_out_assigned_argument(&argc,argv,"insert")) != NULL ) {
if( strcmp(temp,"flat") == 0 ) {
flat_insert = TRUE;
} else {
flat_insert = FALSE;
}
}
if( (strip_out_boolean_argument(&argc,argv,"both")) == TRUE )
do_both = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"fembl")) == TRUE )
is_embl = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"tabs")) == TRUE )
target_abs = TRUE;
pseudo = strip_out_boolean_argument(&argc,argv,"pseudo");
if( (temp = strip_out_assigned_argument(&argc,argv,"codon")) != NULL )
codon_file = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"gene")) != NULL )
gene_file = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"alg")) != NULL )
alg_str = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"kbyte")) != NULL )
kbyte_str = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"subs")) != NULL )
subs_string = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"indel")) != NULL )
indel_string = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"cfreq")) != NULL )
cfreq_string = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"splice")) != NULL ) {
warn("deprecated command line option -splice. use -splice_gtag now");
splice_string = temp;
}
if( (temp = strip_out_assigned_argument(&argc,argv,"init")) != NULL )
startend_string = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"null")) != NULL )
null_string = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"intron")) != NULL )
intron_string = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"alln")) != NULL )
allN_string = temp;
if( (strip_out_boolean_argument(&argc,argv,"hmmer")) == TRUE )
use_tsm = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"intie")) == TRUE )
use_tied_model = TRUE;
if( (temp = strip_out_assigned_argument(&argc,argv,"hname")) != NULL )
hmm_name = temp;
if( (strip_out_boolean_argument(&argc,argv,"pretty")) != FALSE )
show_pretty = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"gff")) != FALSE )
show_gff = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"diana")) != FALSE )
show_diana = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"embl")) != FALSE )
show_embl = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"genes")) != FALSE )
show_pretty_gene = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"genesf")) != FALSE )
show_supp_gene = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"para")) != FALSE )
show_para = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"trans")) != FALSE )
show_trans = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"pep")) != FALSE )
show_pep = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"cdna")) != FALSE )
show_cdna = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"sum")) != FALSE )
show_match_sum = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"alb")) != FALSE )
show_AlnBlock = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"ace")) != FALSE )
show_ace = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"pal")) != FALSE )
show_PackAln = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"gener")) != FALSE )
show_gene_plain = TRUE;
if( (strip_out_boolean_argument(&argc,argv,"over")) != FALSE )
show_overlap = TRUE;
if( (temp = strip_out_assigned_argument(&argc,argv,"divide")) != NULL )
divide_str = temp;
if( (temp = strip_out_assigned_argument(&argc,argv,"block")) != NULL )
main_block_str = temp;
dpri = new_DPRunImpl_from_argv(&argc,argv);
gmp = new_GeneModelParam_from_argv(&argc,argv);
ppp = new_PhasedProteinPara_from_argv(&argc,argv);
gwrp = new_GeneWiseRunPara_from_argv(&argc,argv);
strip_out_remaining_options_with_warning(&argc,argv);
if( argc != 3 ) {
warn("Wrong number of arguments (expect 2)!\n");
if( argc > 1 ){
warn("Arg line looked like (after option processing)");
for(i=1;i<argc;i++) {
fprintf(stderr," %s\n",argv[i]);
}
}
show_short_help();
}
if( show_embl == FALSE && show_diana == FALSE && show_gff == FALSE && show_overlap == FALSE && show_pretty_gene == FALSE && show_match_sum == FALSE && show_ace == FALSE && show_gene_plain == FALSE && show_pretty == FALSE && show_AlnBlock == FALSE && show_PackAln == FALSE && show_pep == FALSE ) {
show_pretty = TRUE;
show_para = TRUE;
}
dna_seq_file = argv[2];
if( use_tsm == FALSE)
protein_file = argv[1];
else
hmm_file = argv[1];
if( build_objects() == FALSE)
fatal("Could not build objects!");
if( show_para == TRUE) {
show_parameters();
}
if( build_alignment() == FALSE)
fatal("Could not build alignment!");
if( show_output() == FALSE)
fatal("Could not show alignment. Sorry!");
if( do_both == TRUE) {
reverse_target();
if( build_alignment() == FALSE)
fatal("Could not build alignment!");
if( show_output() == FALSE)
fatal("Could not show alignment. Sorry!");
}
free_temporary_objects();
free_io_objects();
return 0;
}
int main(int argc,char ** argv)
{
int i;
DPRunImpl * dpri = NULL;
GeneModelParam * gmp = NULL;
GeneModel * gm = NULL;
Sequence * seq;
RandomCodon * rc;
RandomModelDNA * rmd;
RandomCodonScore * rcs;
ComplexSequenceEval * splice5;
ComplexSequenceEval * splice3;
ComplexSequenceEvalSet * cses;
ComplexSequence * cseq;
SyExonScore * exonscore;
PackAln * pal;
AlnBlock * alb;
Genomic * genomic;
GenomicRegion * gr;
Protein * trans;
dpri = new_DPRunImpl_from_argv(&argc,argv);
if( dpri == NULL ) {
fatal("Unable to build DPRun implementation. Bad arguments");
}
gmp = new_GeneModelParam_from_argv(&argc,argv);
ct= read_CodonTable_file("codon.table");
strip_out_standard_options(&argc,argv,show_help,show_version);
if( argc != 2 ) {
show_help(stdout);
exit(12);
}
if((gm=GeneModel_from_GeneModelParam(gmp)) == NULL ) {
fatal("Could not build gene model");
}
seq = read_fasta_file_Sequence(argv[1]);
assert(seq);
cses = new_ComplexSequenceEvalSet_from_GeneModel(gm);
cseq = new_ComplexSequence(seq,cses);
rc = flat_RandomCodon(ct);
rmd = RandomModelDNA_std();
fold_in_RandomModelDNA_into_RandomCodon(rc,rmd);
rcs = RandomCodonScore_from_RandomCodon(rc);
exonscore = SyExonScore_flat_model(200,250,0.1,0.1);
/*
for(i=0;i<cseq->length;i++) {
fprintf(stdout,"%d PairSeq is %d score %d\n",i,CSEQ_PAIR_PAIRBASE(cseq,i),nonc_score->base[CSEQ_PAIR_PAIRBASE(cseq,i)]);
}
exit(0);
*/
/*
show_RandomCodonScore(rcs,stdout);
for(i=3;i<seq->len;i++) {
fprintf(stdout,"seq %d is %c with score %d\n",i,aminoacid_from_seq(ct,seq->seq+i-2),rcs->codon[CSEQ_GENOMIC_CODON(cseq,i)]);
}
exit(0);
*/
pal = PackAln_bestmemory_StatWise10(exonscore,cseq,rcs,Probability2Score(1.0/10.0),Probability2Score(1.0/10.0),NULL,dpri);
alb = convert_PackAln_to_AlnBlock_StatWise10(pal);
mapped_ascii_AlnBlock(alb,id,1,stdout);
genomic = Genomic_from_Sequence(seq);
gr = new_GenomicRegion(genomic);
add_Genes_to_GenomicRegion_GeneWise(gr,1,seq->len,alb,"bollocks",0,NULL);
for(i=0;i<gr->len;i++) {
if( gr->gene[i]->ispseudo == TRUE ) {
fprintf(stdout,"#Gene %d is a pseudo gene - no translation possible\n",i);
} else {
trans = get_Protein_from_Translation(gr->gene[i]->transcript[0]->translation[0],ct);
write_fasta_Sequence(trans->baseseq,stdout);
}
}
return 0;
}
int main(int argc,char ** argv)
{
int i;
DPRunImpl * dpri = NULL;
GeneModelParam * gmp = NULL;
GeneModel * gm = NULL;
FILE * ifp;
SeqAlign * al;
PairBaseSeq * pbs;
ComplexSequenceEval * splice5;
ComplexSequenceEval * splice3;
ComplexSequence * cseq;
CompMat * score_mat;
CompProb * comp_prob;
RandomModel * rm;
PairBaseCodonModelScore * codon_score;
PairBaseModelScore* nonc_score;
PairBaseCodonModelScore * start;
PairBaseCodonModelScore * stop;
SyExonScore * exonscore;
PackAln * pal;
AlnBlock * alb;
Genomic * genomic;
GenomicRegion * gr;
GenomicRegion * gr2;
Protein * trans;
StandardOutputOptions * std_opt;
ShowGenomicRegionOptions * sgro;
char * dump_packaln = NULL;
char * read_packaln = NULL;
FILE * packifp = NULL;
boolean show_trans = 1;
boolean show_gene_raw = 0;
ct = read_CodonTable_file(codon_table);
/*
score_mat = read_Blast_file_CompMat("blosum62.bla");
comp_prob = CompProb_from_halfbit(score_mat);
*/
rm = default_RandomModel();
comp_prob = read_Blast_file_CompProb("wag85");
fold_column_RandomModel_CompProb(comp_prob,rm);
dpri = new_DPRunImpl_from_argv(&argc,argv);
if( dpri == NULL ) {
fatal("Unable to build DPRun implementation. Bad arguments");
}
gmp = new_GeneModelParam_from_argv(&argc,argv);
std_opt = new_StandardOutputOptions_from_argv(&argc,argv);
sgro = new_ShowGenomicRegionOptions_from_argv(&argc,argv);
dump_packaln = strip_out_assigned_argument(&argc,argv,"dump");
read_packaln = strip_out_assigned_argument(&argc,argv,"recover");
strip_out_standard_options(&argc,argv,show_help,show_version);
if( argc != 2 ) {
show_help(stdout);
exit(12);
}
if((gm=GeneModel_from_GeneModelParam(gmp)) == NULL ) {
fatal("Could not build gene model");
}
codon_score = make_PairBaseCodonModelScore(comp_prob);
nonc_score = make_PairBaseModelScore();
splice5 = ComplexSequenceEval_from_pwmDNAScore_splice(gm->splice5score);
splice3 = ComplexSequenceEval_from_pwmDNAScore_splice(gm->splice3score);
if((ifp = openfile(argv[1],"r")) == NULL ) {
fatal("Could not open file %s",argv[1]);
}
al = read_fasta_SeqAlign(ifp);
assert(al);
assert(al->len == 2);
assert(al->seq[0]->len > 0);
assert(al->seq[1]->len > 0);
/* write_fasta_SeqAlign(al,stdout);*/
pbs = new_PairBaseSeq_SeqAlign(al);
if( read_packaln == NULL ) {
cseq = ComplexSequence_from_PairBaseSeq(pbs,splice5,splice3);
}
start = make_start_PairBaseCodonModelScore(ct);
stop = make_stop_PairBaseCodonModelScore(ct);
/* show_PairBaseCodonModelScore(stop,ct,stdout); */
/*
for(i=0;i<pbs->anchor->len;i++) {
printf("%3d %c For %-6d %-6d %c Rev %-6d %-6d\n",i,pbs->anchor->seq[i],
CSEQ_PAIR_5SS(cseq,i),CSEQ_PAIR_3SS(cseq,i),
char_complement_base(pbs->anchor->seq[i]),
CSEQ_REV_PAIR_5SS(cseq,i),CSEQ_REV_PAIR_3SS(cseq,i));
}
*/
/* show_ComplexSequence(cseq,stdout);
*/
exonscore = SyExonScore_flat_model(100,150,0.1,1.0);
/*
for(i=0;i<cseq->length;i++) {
fprintf(stdout,"%d PairSeq is %d score %d\n",i,CSEQ_PAIR_PAIRBASE(cseq,i),nonc_score->base[CSEQ_PAIR_PAIRBASE(cseq,i)]);
}
exit(0);
*/
if( read_packaln != NULL ) {
packifp = openfile(read_packaln,"r");
if( packifp == NULL ) {
fatal("File %s is unopenable - ignoring dump command",dump_packaln);
} else {
pal = read_simple_PackAln(packifp);
}
} else {
pal = PackAln_bestmemory_SyWise20(exonscore,cseq,codon_score,nonc_score,start,stop,Probability2Score(1.0/100.0),Probability2Score(1.0/10000.0),Probability2Score(1.0/10.0),NULL,dpri);
}
alb = convert_PackAln_to_AlnBlock_SyWise20(pal);
if( dump_packaln != NULL ) {
packifp = openfile(dump_packaln,"w");
if( packifp == NULL ) {
warn("File %s is unopenable - ignoring dump command",dump_packaln);
} else {
show_simple_PackAln(pal,packifp);
}
}
show_score_sequence(alb,pbs,nonc_score,stdout);
/*
show_StandardOutputOptions(std_opt,alb,pal,"//",stdout);
*/
genomic = Genomic_from_Sequence(al->seq[0]);
gr = new_GenomicRegion(genomic);
gr2 = new_GenomicRegion(genomic);
add_Genes_to_GenomicRegion_new(gr,alb);
show_GenomicRegionOptions(sgro,gr,ct,"//",stdout);
return 0;
}
int main(int argc,char ** argv)
{
Sequence * cdna;
Sequence * gen;
Sequence * active_gen;
Sequence * active_cdna;
int i;
int dstart = -1;
int dend = -1;
int cstart = -1;
int cend = -1;
CodonTable * ct = NULL;
CodonMatrixScore * cm = NULL;
RandomCodon * rndcodon = NULL;
RandomCodonScore * rndcodonscore = NULL;
DnaMatrix * dm = NULL;
DPRunImpl * dpri = NULL;
GeneModel * gm;
GeneModelParam * gmp;
GeneStats * gs;
GeneParser21 * gp21;
GeneParser21Score * gp21s;
GeneParser4Score * gp;
ComplexSequenceEvalSet * cdna_cses;
ComplexSequenceEvalSet * gen_cses;
ComplexSequence * cs_cdna;
ComplexSequence * cs_gen;
Genomic * gent;
GenomicRegion * gr;
CompMat * cmat;
CompProb * cprob;
char * matfile = "blosum62.bla";
Protein * trans;
PackAln * pal;
AlnBlock * alb;
FILE * ofp = stdout;
dpri = new_DPRunImpl_from_argv(&argc,argv);
gmp = new_GeneModelParam_from_argv(&argc,argv);
strip_out_integer_argument(&argc,argv,"u",&dstart);
strip_out_integer_argument(&argc,argv,"v",&dend);
strip_out_integer_argument(&argc,argv,"s",&cstart);
strip_out_integer_argument(&argc,argv,"t",&cend);
strip_out_standard_options(&argc,argv,show_help,show_version);
ct = read_CodonTable_file(codon_file);
cmat = read_Blast_file_CompMat(matfile);
cprob = CompProb_from_halfbit(cmat);
cm = naive_CodonMatrixScore_from_prob(ct,cprob);
gm = GeneModel_from_GeneModelParam(gmp);
cdna = read_fasta_file_Sequence(argv[1]);
gen = read_fasta_file_Sequence(argv[2]);
if( dstart != -1 || dend != -1 ) {
if( dstart == -1 ) {
dstart = 1;
}
if( dend == -1 ) {
dend = gen->len;
}
active_gen = magic_trunc_Sequence(gen,dstart,dend);
} else {
active_gen = hard_link_Sequence(gen);
}
if( cstart != -1 || cend != -1 ) {
if( cstart == -1 ) {
cstart = 1;
}
if( cend == -1 ) {
cend = gen->len;
}
active_cdna = magic_trunc_Sequence(gen,cstart,cend);
} else {
active_cdna = hard_link_Sequence(gen);
}
rndcodon = RandomCodon_from_raw_CodonFrequency(gm->codon,ct);
fold_in_RandomModelDNA_into_RandomCodon(rndcodon,gm->rnd);
rndcodonscore = RandomCodonScore_from_RandomCodon(rndcodon);
assert(active_cdna);
assert(active_gen);
cdna_cses = default_cDNA_ComplexSequenceEvalSet();
gen_cses = new_ComplexSequenceEvalSet_from_GeneModel(gm);
cs_cdna = new_ComplexSequence(active_cdna,cdna_cses);
cs_gen = new_ComplexSequence(active_gen,gen_cses);
gp21 = std_GeneParser21();
GeneParser21_fold_in_RandomModelDNA(gp21,gm->rnd);
gp21s = GeneParser21Score_from_GeneParser21(gp21);
gp = GeneParser4Score_from_GeneParser21Score(gp21s);
dm = identity_DnaMatrix(Probability2Score(halfbit2Probability(1)),Probability2Score(halfbit2Probability(-1)));
assert(cs_cdna);
assert(cs_gen);
assert(gp);
assert(rndcodonscore);
assert(dm);
assert(dpri);
/* show_CodonMatrixScore(cm,ct,ofp);*/
pal = PackAln_bestmemory_CdnaWise10(cs_cdna,cs_gen,gp,cm,rndcodonscore,dm,
Probability2Score(halfbit2Probability(-12)),
Probability2Score(halfbit2Probability(-2)),
Probability2Score(halfbit2Probability(-5)),
Probability2Score(halfbit2Probability(0)),
NULL,
dpri);
alb = convert_PackAln_to_AlnBlock_CdnaWise10(pal);
gent = Genomic_from_Sequence(gen);
assert(gent);
gr = new_GenomicRegion(gent);
assert(gr);
add_Genes_to_GenomicRegion_GeneWise(gr,active_gen->offset,active_gen->end,alb,cdna->name,0,NULL);
mapped_ascii_AlnBlock(alb,Score2Bits,0,ofp);
show_pretty_GenomicRegion(gr,0,ofp);
for(i=0;i<gr->len;i++) {
if( gr->gene[i]->ispseudo == TRUE ) {
fprintf(ofp,"#Gene %d is a pseudo gene - no translation possible\n",i);
} else {
trans = get_Protein_from_Translation(gr->gene[i]->transcript[0]->translation[0],ct);
write_fasta_Sequence(trans->baseseq,ofp);
}
}
}