CellPtr Cell::Divide()
{
// Check we're allowed to divide
assert(!IsDead());
assert(mCanDivide);
mCanDivide = false;
// Reset properties of parent cell
mpCellCycleModel->ResetForDivision();
mpSrnModel->ResetForDivision();
// Create copy of cell property collection to modify for daughter cell
CellPropertyCollection daughter_property_collection = mCellPropertyCollection;
// Remove the CellId from the daughter cell, as a new one will be assigned in the constructor
daughter_property_collection.RemoveProperty<CellId>();
// Copy all cell data (note we create a new object not just copying the pointer)
assert(daughter_property_collection.HasPropertyType<CellData>());
// Get the existing copy of the cell data and remove it from the daughter cell
boost::shared_ptr<CellData> p_cell_data = GetCellData();
daughter_property_collection.RemoveProperty(p_cell_data);
// Create a new cell data object using the copy constructor and add this to the daughter cell
MAKE_PTR_ARGS(CellData, p_daughter_cell_data, (*p_cell_data));
daughter_property_collection.AddProperty(p_daughter_cell_data);
// Copy all cell Vec data (note we create a new object not just copying the pointer)
if (daughter_property_collection.HasPropertyType<CellVecData>())
{
// Get the existing copy of the cell data and remove it from the daughter cell
boost::shared_ptr<CellVecData> p_cell_vec_data = GetCellVecData();
daughter_property_collection.RemoveProperty(p_cell_vec_data);
// Create a new cell data object using the copy constructor and add this to the daughter cell
MAKE_PTR_ARGS(CellVecData, p_daughter_cell_vec_data, (*p_cell_vec_data));
daughter_property_collection.AddProperty(p_daughter_cell_vec_data);
}
// Create daughter cell with modified cell property collection
CellPtr p_new_cell(new Cell(GetMutationState(), mpCellCycleModel->CreateCellCycleModel(), mpSrnModel->CreateSrnModel(), false, daughter_property_collection));
// Initialise properties of daughter cell
p_new_cell->GetCellCycleModel()->InitialiseDaughterCell();
p_new_cell->GetSrnModel()->InitialiseDaughterCell();
// Set the daughter cell to inherit the apoptosis time of the parent cell
p_new_cell->SetApoptosisTime(mApoptosisTime);
return p_new_cell;
}
void TestAddCellWithShovingBasedDivisionRuleAndShovingRequired()
{
/**
* In this test of ShovingCaBasedDivisionRule we check the case where there is
* no room to divide without the cells being shoved to the edge of the mesh.
*/
// Create a simple Potts mesh
PottsMeshGenerator<2> generator(5, 0, 0, 5, 0, 0);
PottsMesh<2>* p_mesh = generator.GetMesh();
// Create 25 cells, one for each node
std::vector<unsigned> location_indices;
for (unsigned index=0; index<25; index++)
{
location_indices.push_back(index);
}
std::vector<CellPtr> cells;
CellsGenerator<FixedG1GenerationalCellCycleModel, 1> cells_generator;
cells_generator.GenerateBasic(cells, location_indices.size());
// Create cell population
CaBasedCellPopulation<2> cell_population(*p_mesh, cells, location_indices);
// Make a new cell to add
MAKE_PTR(WildTypeCellMutationState, p_state);
MAKE_PTR(StemCellProliferativeType, p_stem_type);
FixedG1GenerationalCellCycleModel* p_model = new FixedG1GenerationalCellCycleModel();
CellPtr p_new_cell(new Cell(p_state, p_model));
p_new_cell->SetCellProliferativeType(p_stem_type);
p_new_cell->SetBirthTime(-1);
// Set the division rule for our population to be the shoving division rule
boost::shared_ptr<AbstractCaBasedDivisionRule<2> > p_division_rule_to_set(new ShovingCaBasedDivisionRule<2>());
cell_population.SetCaBasedDivisionRule(p_division_rule_to_set);
// Get the division rule back from the population and try to add new cell by dividing cell at site 0;
boost::shared_ptr<AbstractCaBasedDivisionRule<2> > p_division_rule = cell_population.GetCaBasedDivisionRule();
// Select central cell
CellPtr p_cell_12 = cell_population.GetCellUsingLocationIndex(12);
// Try to divide but cant as hit boundary
TS_ASSERT_THROWS_THIS(p_division_rule->CalculateDaughterNodeIndex(p_new_cell, p_cell_12, cell_population),
"Cells reaching the boundary of the domain. Make the Potts mesh larger.");
}
void TestAddCell() throw(Exception)
{
// Create a simple 2D PottsMesh with one cell
PottsMeshGenerator<2> generator(2, 1, 2, 2, 1, 2);
PottsMesh<2>* p_mesh = generator.GetMesh();
// Create cells
std::vector<CellPtr> cells;
CellsGenerator<FixedDurationGenerationBasedCellCycleModel, 2> cells_generator;
cells_generator.GenerateBasic(cells, p_mesh->GetNumElements());
// Create cell population
PottsBasedCellPopulation<2> cell_population(*p_mesh, cells);
// Test we have the correct number of cells and elements
TS_ASSERT_EQUALS(cell_population.GetNumElements(), 1u);
TS_ASSERT_EQUALS(cell_population.rGetCells().size(), 1u);
// Create a new cell
MAKE_PTR(WildTypeCellMutationState, p_state);
MAKE_PTR(StemCellProliferativeType, p_stem_type);
FixedDurationGenerationBasedCellCycleModel* p_model = new FixedDurationGenerationBasedCellCycleModel();
CellPtr p_new_cell(new Cell(p_state, p_model));
p_new_cell->SetCellProliferativeType(p_stem_type);
// Add new cell to the cell population by dividing the cell
AbstractCellPopulation<2>::Iterator cell_iter_1 = cell_population.Begin();
cell_population.AddCell(p_new_cell, zero_vector<double>(2), *cell_iter_1);
TS_ASSERT_EQUALS(cell_population.GetLocationIndexUsingCell(p_new_cell), 1u);
TS_ASSERT_EQUALS(cell_population.rGetCells().size(), 2u);
TS_ASSERT_EQUALS(cell_population.GetNumRealCells(), 2u);
// Check locations of parent and daughter cell
AbstractCellPopulation<2>::Iterator cell_iter_2 = cell_population.Begin();
TS_ASSERT_EQUALS(cell_population.GetLocationIndexUsingCell(*cell_iter_2), 0u);
++cell_iter_2;
TS_ASSERT_EQUALS(cell_population.GetLocationIndexUsingCell(*cell_iter_2), 1u);
// Check Elements are as expected
TS_ASSERT_EQUALS(cell_population.rGetMesh().GetElement(0)->GetNodeGlobalIndex(0), 0u);
TS_ASSERT_EQUALS(cell_population.rGetMesh().GetElement(0)->GetNodeGlobalIndex(1), 1u);
TS_ASSERT_EQUALS(cell_population.rGetMesh().GetElement(1)->GetNodeGlobalIndex(0), 2u);
TS_ASSERT_EQUALS(cell_population.rGetMesh().GetElement(1)->GetNodeGlobalIndex(1), 3u);
}
void TestAddCellWithShovingBasedDivisionRule()
{
/**
* In this test we create a new ShovingCaBasedDivisionRule, divide a cell with it
* and check that the new cells are in the correct locations. First, we test where
* there is space around the cells. This is the default setup.
*/
// Create a simple Potts mesh
PottsMeshGenerator<2> generator(5, 0, 0, 5, 0, 0);
PottsMesh<2>* p_mesh = generator.GetMesh();
// Create 9 cells in the central nodes
std::vector<unsigned> location_indices;
for (unsigned row=1; row<4; row++)
{
location_indices.push_back(1+row*5);
location_indices.push_back(2+row*5);
location_indices.push_back(3+row*5);
}
std::vector<CellPtr> cells;
CellsGenerator<FixedG1GenerationalCellCycleModel, 1> cells_generator;
cells_generator.GenerateBasic(cells, location_indices.size());
// Create cell population
CaBasedCellPopulation<2> cell_population(*p_mesh, cells, location_indices);
// Check the cell locations
unsigned cell_locations[9] = {6, 7, 8, 11, 12, 13, 16, 17, 18};
unsigned index = 0;
for (AbstractCellPopulation<2>::Iterator cell_iter = cell_population.Begin();
cell_iter != cell_population.End();
++cell_iter)
{
TS_ASSERT_EQUALS(cell_population.GetLocationIndexUsingCell(*cell_iter),cell_locations[index])
++index;
}
// Make a new cell to add
MAKE_PTR(WildTypeCellMutationState, p_state);
MAKE_PTR(StemCellProliferativeType, p_stem_type);
FixedG1GenerationalCellCycleModel* p_model = new FixedG1GenerationalCellCycleModel();
CellPtr p_new_cell(new Cell(p_state, p_model));
p_new_cell->SetCellProliferativeType(p_stem_type);
p_new_cell->SetBirthTime(-1);
// Set the division rule for our population to be the shoving division rule
boost::shared_ptr<AbstractCaBasedDivisionRule<2> > p_division_rule_to_set(new ShovingCaBasedDivisionRule<2>());
cell_population.SetCaBasedDivisionRule(p_division_rule_to_set);
// Get the division rule back from the population and try to add new cell by dividing cell at site 0
boost::shared_ptr<AbstractCaBasedDivisionRule<2> > p_division_rule = cell_population.GetCaBasedDivisionRule();
// Select central cell
CellPtr p_cell_12 = cell_population.GetCellUsingLocationIndex(12);
// The ShovingCaBasedDivisionRule method IsRoomToDivide() always returns true
TS_ASSERT_EQUALS((p_division_rule->IsRoomToDivide(p_cell_12, cell_population)), true);
/*
* Test adding the new cell to the population; this calls CalculateDaughterNodeIndex().
* The new cell moves into node 13.
*/
cell_population.AddCell(p_new_cell, p_cell_12);
// Now check the cells are in the correct place
TS_ASSERT_EQUALS(cell_population.GetNumRealCells(), 10u);
// Note the cell originally on node 13 has been shoved to node 14 and the new cell is on node 13
unsigned new_cell_locations[10] = {6, 7, 8, 11, 12, 14, 16, 17, 18, 13};
index = 0;
for (AbstractCellPopulation<2>::Iterator cell_iter = cell_population.Begin();
cell_iter != cell_population.End();
++cell_iter)
{
TS_ASSERT_EQUALS(cell_population.GetLocationIndexUsingCell(*cell_iter), new_cell_locations[index])
++index;
}
}
void MeshBasedCellPopulation<ELEMENT_DIM,SPACE_DIM>::DivideLongSprings(double springDivisionThreshold)
{
// Only implemented for 2D elements
assert(ELEMENT_DIM==2);
std::vector<c_vector<unsigned, 5> > new_nodes;
new_nodes = rGetMesh().SplitLongEdges(springDivisionThreshold);
// Add new cells onto new nodes
for (unsigned index=0; index<new_nodes.size(); index++)
{
// Copy the cell attached to one of the neighbouring nodes onto the new node
unsigned new_node_index = new_nodes[index][0];
unsigned node_a_index = new_nodes[index][1];
unsigned node_b_index = new_nodes[index][2];
CellPtr p_neighbour_cell = this->GetCellUsingLocationIndex(node_a_index);
// Create copy of cell property collection to modify for daughter cell
CellPropertyCollection daughter_property_collection = p_neighbour_cell->rGetCellPropertyCollection();
// Remove the CellId from the daughter cell a new one will be assigned in the constructor
daughter_property_collection.RemoveProperty<CellId>();
CellPtr p_new_cell(new Cell(p_neighbour_cell->GetMutationState(),
p_neighbour_cell->GetCellCycleModel()->CreateCellCycleModel(),
p_neighbour_cell->GetSrnModel()->CreateSrnModel(),
false,
daughter_property_collection));
// Add new cell to cell population
this->mCells.push_back(p_new_cell);
this->AddCellUsingLocationIndex(new_node_index,p_new_cell);
// Update rest lengths
// Remove old node pair // note node_a_index < node_b_index
std::pair<unsigned,unsigned> node_pair = this->CreateOrderedPair(node_a_index, node_b_index);
double old_rest_length = mSpringRestLengths[node_pair];
std::map<std::pair<unsigned,unsigned>, double>::iterator iter = mSpringRestLengths.find(node_pair);
mSpringRestLengths.erase(iter);
// Add new pairs
node_pair = this->CreateOrderedPair(node_a_index, new_node_index);
mSpringRestLengths[node_pair] = 0.5*old_rest_length;
node_pair = this->CreateOrderedPair(node_b_index, new_node_index);
mSpringRestLengths[node_pair] = 0.5*old_rest_length;
// If necessary add other new spring rest lengths
for (unsigned pair_index=3; pair_index<5; pair_index++)
{
unsigned other_node_index = new_nodes[index][pair_index];
if (other_node_index != UNSIGNED_UNSET)
{
node_pair = this->CreateOrderedPair(other_node_index, new_node_index);
double new_rest_length = rGetMesh().GetDistanceBetweenNodes(new_node_index, other_node_index);
mSpringRestLengths[node_pair] = new_rest_length;
}
}
}
}
void TestAddAndRemoveAndAddWithOutUpdate()
{
EXIT_IF_PARALLEL; // This test doesn't work in parallel.
SimulationTime* p_simulation_time = SimulationTime::Instance();
p_simulation_time->SetEndTimeAndNumberOfTimeSteps(10.0, 1);
// Create a simple mesh
TrianglesMeshReader<2,2> mesh_reader("mesh/test/data/square_128_elements");
TetrahedralMesh<2,2> generating_mesh;
generating_mesh.ConstructFromMeshReader(mesh_reader);
// Convert this to a NodesOnlyMesh
NodesOnlyMesh<2> mesh;
mesh.ConstructNodesWithoutMesh(generating_mesh, 1.2);
// Create vector of cell location indices
std::vector<unsigned> cell_location_indices;
for (unsigned i=10; i<mesh.GetNumNodes(); i++)
{
if (i != 80)
{
cell_location_indices.push_back(i);
}
}
// Set up cells
std::vector<CellPtr> cells;
CellsGenerator<FixedDurationGenerationBasedCellCycleModel, 2> cells_generator;
cells_generator.GenerateBasic(cells, cell_location_indices.size());
cells[27]->StartApoptosis();
// Create a cell population, with some random particles
NodeBasedCellPopulationWithParticles<2> cell_population(mesh, cells, cell_location_indices);
TS_ASSERT_EQUALS(mesh.GetNumNodes(), 81u);
TS_ASSERT_EQUALS(cell_population.rGetCells().size(), 70u);
MAKE_PTR(WildTypeCellMutationState, p_state);
MAKE_PTR(StemCellProliferativeType, p_stem_type);
FixedDurationGenerationBasedCellCycleModel* p_model = new FixedDurationGenerationBasedCellCycleModel();
CellPtr p_new_cell(new Cell(p_state, p_model));
p_new_cell->SetCellProliferativeType(p_stem_type);
p_new_cell->SetBirthTime(0);
c_vector<double,2> new_location = zero_vector<double>(2);
new_location[0] = 0.3433453454443;
new_location[0] = 0.3435346344234;
cell_population.AddCell(p_new_cell, new_location, cell_population.rGetCells().front() /*random choice of parent*/);
TS_ASSERT_EQUALS(mesh.GetNumNodes(), 82u);
TS_ASSERT_EQUALS(cell_population.GetNumRealCells(), 71u);
p_simulation_time->IncrementTimeOneStep();
unsigned num_removed = cell_population.RemoveDeadCells();
TS_ASSERT_EQUALS(num_removed, 1u);
TS_ASSERT_EQUALS(mesh.GetNumNodes(), 81u);
TS_ASSERT_EQUALS(cell_population.GetNumRealCells(), 70u);
FixedDurationGenerationBasedCellCycleModel* p_model2 = new FixedDurationGenerationBasedCellCycleModel();
CellPtr p_new_cell2(new Cell(p_state, p_model2));
p_new_cell2->SetCellProliferativeType(p_stem_type);
p_new_cell2->SetBirthTime(0);
c_vector<double,2> new_location2 = zero_vector<double>(2);
new_location2[0] = 0.6433453454443;
new_location2[0] = 0.6435346344234;
cell_population.AddCell(p_new_cell2, new_location2, cell_population.rGetCells().front() /*random choice of parent*/);
TS_ASSERT_EQUALS(mesh.GetNumNodes(), 82u);
TS_ASSERT_EQUALS(cell_population.GetNumRealCells(), 71u);
}
