static void connectPreNodeToTheNext(IDnum * currentPreNodeID,
IDnum nextPreNodeID,
Coordinate * currentPosition,
IDnum sequenceIndex,
boolean isReference,
PreGraph * preGraph)
{
if (nextPreNodeID == 0)
return;
#ifdef _OPENMP
lockTwoNodes(*currentPreNodeID, nextPreNodeID);
#endif
if (isReference)
incrementNodeReferenceMarkerCount_pg(preGraph, nextPreNodeID);
if (!isReference && *currentPreNodeID != 0)
createPreArc_pg(*currentPreNodeID, nextPreNodeID,
preGraph);
#ifdef _OPENMP
unLockTwoNodes(*currentPreNodeID, nextPreNodeID);
#endif
*currentPreNodeID = nextPreNodeID;
*currentPosition +=
getPreNodeLength_pg(*currentPreNodeID, preGraph);
}
static void threadSequenceThroughGraph(TightString * tString,
KmerOccurenceTable * kmerTable,
Graph * graph,
IDnum seqID, Category category,
boolean readTracking,
boolean double_strand,
ReferenceMapping * referenceMappings,
Coordinate referenceMappingCount,
IDnum refCount,
Annotation * annotations,
IDnum annotationCount,
boolean second_in_pair)
{
Kmer word;
Kmer antiWord;
Coordinate readNucleotideIndex;
Coordinate kmerIndex;
KmerOccurence *kmerOccurence;
int wordLength = getWordLength(graph);
PassageMarkerI marker = NULL_IDX;
PassageMarkerI previousMarker = NULL_IDX;
Node *node = NULL;
Node *previousNode = NULL;
Coordinate coord = 0;
Coordinate previousCoord = 0;
Nucleotide nucleotide;
boolean reversed;
IDnum refID;
Coordinate refCoord = 0;
ReferenceMapping * refMap;
Annotation * annotation = annotations;
Coordinate index = 0;
Coordinate uniqueIndex = 0;
Coordinate annotIndex = 0;
IDnum annotCount = 0;
SmallNodeList * nodePile = NULL;
// Neglect any string shorter than WORDLENGTH :
if (getLength(tString) < wordLength)
return;
clearKmer(&word);
clearKmer(&antiWord);
// Fill in the initial word :
for (readNucleotideIndex = 0;
readNucleotideIndex < wordLength - 1; readNucleotideIndex++) {
nucleotide = getNucleotide(readNucleotideIndex, tString);
pushNucleotide(&word, nucleotide);
if (double_strand || second_in_pair) {
#ifdef COLOR
reversePushNucleotide(&antiWord, nucleotide);
#else
reversePushNucleotide(&antiWord, 3 - nucleotide);
#endif
}
}
// Go through sequence
while (readNucleotideIndex < getLength(tString)) {
nucleotide = getNucleotide(readNucleotideIndex++, tString);
pushNucleotide(&word, nucleotide);
if (double_strand || second_in_pair) {
#ifdef COLOR
reversePushNucleotide(&antiWord, nucleotide);
#else
reversePushNucleotide(&antiWord, 3 - nucleotide);
#endif
}
// Update annotation if necessary
if (annotCount < annotationCount && annotIndex == getAnnotationLength(annotation)) {
annotation = getNextAnnotation(annotation);
annotCount++;
annotIndex = 0;
}
// Search for reference mapping
if (category == REFERENCE) {
if (referenceMappings)
refMap = findReferenceMapping(seqID, index, referenceMappings, referenceMappingCount);
else
refMap = NULL;
if (refMap) {
node = getNodeInGraph(graph, refMap->nodeID);
if (refMap->nodeID > 0) {
coord = refMap->nodeStart + (index - refMap->referenceStart);
} else {
coord = getNodeLength(node) - refMap->nodeStart - refMap->length + (index - refMap->referenceStart);
}
} else {
node = NULL;
if (previousNode)
break;
}
}
// Search for reference-based mapping
else if (annotCount < annotationCount && uniqueIndex >= getPosition(annotation) && getAnnotSequenceID(annotation) <= refCount && getAnnotSequenceID(annotation) >= -refCount) {
refID = getAnnotSequenceID(annotation);
if (refID > 0)
refCoord = getStart(annotation) + annotIndex;
else
refCoord = getStart(annotation) - annotIndex;
refMap = findReferenceMapping(refID, refCoord, referenceMappings, referenceMappingCount);
// If success
if (refMap) {
if (refID > 0) {
node = getNodeInGraph(graph, refMap->nodeID);
if (refMap->nodeID > 0) {
coord = refMap->nodeStart + (refCoord - refMap->referenceStart);
} else {
coord = getNodeLength(node) - refMap->nodeStart - refMap->length + (refCoord - refMap->referenceStart);
}
} else {
node = getNodeInGraph(graph, -refMap->nodeID);
if (refMap->nodeID > 0) {
coord = getNodeLength(node) - refMap->nodeStart - (refCoord - refMap->referenceStart) - 1;
} else {
coord = refMap->nodeStart + refMap->length - (refCoord - refMap->referenceStart) - 1;
}
}
} else {
node = NULL;
if (previousNode)
break;
}
}
// Search in table
else {
reversed = false;
if (double_strand) {
if (compareKmers(&word, &antiWord) <= 0) {
kmerOccurence =
findKmerInKmerOccurenceTable(&word,
kmerTable);
} else {
kmerOccurence =
findKmerInKmerOccurenceTable(&antiWord,
kmerTable);
reversed = true;
}
} else {
if (!second_in_pair) {
kmerOccurence =
findKmerInKmerOccurenceTable(&word,
kmerTable);
} else {
kmerOccurence =
findKmerInKmerOccurenceTable(&antiWord,
kmerTable);
reversed = true;
}
}
if (kmerOccurence) {
if (!reversed) {
node = getNodeInGraph(graph, getKmerOccurenceNodeID(kmerOccurence));
coord = getKmerOccurencePosition(kmerOccurence);
} else {
node = getNodeInGraph(graph, -getKmerOccurenceNodeID(kmerOccurence));
coord = getNodeLength(node) - getKmerOccurencePosition(kmerOccurence) - 1;
}
} else {
node = NULL;
if (previousNode)
break;
}
}
// Increment positions
if (annotCount < annotationCount && uniqueIndex >= getPosition(annotation))
annotIndex++;
else
uniqueIndex++;
// Fill in graph
if (node)
{
#ifdef OPENMP
lockNode(node);
#endif
kmerIndex = readNucleotideIndex - wordLength;
if (previousNode == node
&& previousCoord == coord - 1) {
if (category / 2 >= CATEGORIES) {
setPassageMarkerFinish(marker,
kmerIndex +
1);
setFinishOffset(marker,
getNodeLength(node)
- coord - 1);
} else {
#ifndef SINGLE_COV_CAT
incrementVirtualCoverage(node, category / 2, 1);
incrementOriginalVirtualCoverage(node, category / 2, 1);
#else
incrementVirtualCoverage(node, 1);
#endif
}
#ifdef OPENMP
unLockNode(node);
#endif
} else {
if (category / 2 >= CATEGORIES) {
marker =
newPassageMarker(seqID,
kmerIndex,
kmerIndex + 1,
coord,
getNodeLength
(node) -
coord - 1);
transposePassageMarker(marker,
node);
connectPassageMarkers
(previousMarker, marker,
graph);
previousMarker = marker;
} else {
if (readTracking) {
if (!isNodeMemorized(node, nodePile)) {
addReadStart(node,
seqID,
coord,
graph,
kmerIndex);
memorizeNode(node, &nodePile);
} else {
blurLastShortReadMarker
(node, graph);
}
}
#ifndef SINGLE_COV_CAT
incrementVirtualCoverage(node, category / 2, 1);
incrementOriginalVirtualCoverage(node, category / 2, 1);
#else
incrementVirtualCoverage(node, 1);
#endif
}
#ifdef OPENMP
lockTwoNodes(node, previousNode);
#endif
createArc(previousNode, node, graph);
#ifdef OPENMP
unLockTwoNodes(node, previousNode);
#endif
}
previousNode = node;
previousCoord = coord;
}
index++;
}
if (readTracking && category / 2 < CATEGORIES)
unMemorizeNodes(&nodePile);
}