/* Function: main_loop_threaded() * Date: SRE, Wed Dec 1 12:43:09 1999 [St. Louis] * * Purpose: Given an HMM and parameters for synthesizing random * sequences; return a histogram of scores. * (Threaded version.) * * Args: hmm - an HMM to calibrate. * seed - random number seed * nsample - number of seqs to synthesize * lenmean - mean length of random sequence * lensd - std dev of random seq length * fixedlen - if nonzero, override lenmean, always this len * nthreads - number of threads to start * ret_hist - RETURN: the score histogram * ret_max - RETURN: highest score seen in simulation * twatch - RETURN: accumulation of thread times * * Returns: (void) * hist is alloc'ed here, and must be free'd by caller. */ static void main_loop_threaded(struct plan7_s *hmm, int seed, int nsample, float lenmean, float lensd, int fixedlen, int nthreads, struct histogram_s **ret_hist, float *ret_max, Stopwatch_t *twatch) { struct histogram_s *hist; float randomseq[MAXABET]; float p1; struct workpool_s *wpool; /* pool of worker threads */ /* Initialize. * We assume we've already set the alphabet (safe, because * HMM input sets the alphabet). */ sre_srandom(seed); P7Logoddsify(hmm, TRUE); P7DefaultNullModel(randomseq, &p1); hist = AllocHistogram(-200, 200, 100); wpool = workpool_start(hmm, lenmean, lensd, fixedlen, randomseq, nsample, hist, nthreads); workpool_stop(wpool); *ret_hist = hist; *ret_max = wpool->max_score; StopwatchInclude(twatch, &(wpool->watch)); workpool_free(wpool); return; }
/* Function: main_loop_serial() * Date: SRE, Tue Aug 18 16:18:28 1998 [St. Louis] * * Purpose: Given an HMM and parameters for synthesizing random * sequences; return a histogram of scores. * (Serial version) * * Args: hmm - an HMM to calibrate. * seed - random number seed * nsample - number of seqs to synthesize * lenmean - mean length of random sequence * lensd - std dev of random seq length * fixedlen - if nonzero, override lenmean, always this len * ret_hist - RETURN: the score histogram * ret_max - RETURN: highest score seen in simulation * * Returns: (void) * hist is alloc'ed here, and must be free'd by caller. */ static void main_loop_serial(struct plan7_s *hmm, int seed, int nsample, float lenmean, float lensd, int fixedlen, struct histogram_s **ret_hist, float *ret_max) { struct histogram_s *hist; float randomseq[MAXABET]; float p1; float max; char *seq; char *dsq; float score; int sqlen; int idx; /* Initialize. * We assume we've already set the alphabet (safe, because * HMM input sets the alphabet). */ sre_srandom(seed); P7Logoddsify(hmm, TRUE); P7DefaultNullModel(randomseq, &p1); hist = AllocHistogram(-200, 200, 100); max = -FLT_MAX; for (idx = 0; idx < nsample; idx++) { /* choose length of random sequence */ if (fixedlen) sqlen = fixedlen; else do sqlen = (int) Gaussrandom(lenmean, lensd); while (sqlen < 1); /* generate it */ seq = RandomSequence(Alphabet, randomseq, Alphabet_size, sqlen); dsq = DigitizeSequence(seq, sqlen); if (P7ViterbiSize(sqlen, hmm->M) <= RAMLIMIT) score = P7Viterbi(dsq, sqlen, hmm, NULL); else score = P7SmallViterbi(dsq, sqlen, hmm, NULL); AddToHistogram(hist, score); if (score > max) max = score; free(dsq); free(seq); } *ret_hist = hist; *ret_max = max; return; }
void HMMCreateWPoolTask::runUnsafe() { const UHMMCalibrateSettings& settings = pt->getSettings(); WorkPool_s* wpool = pt->getWorkPool(); SetAlphabet(wpool->hmm->atype); sre_srandom(settings.seed); wpool->fixedlen = settings.fixedlen; wpool->hist = AllocHistogram(-200, 200, 100); wpool->lenmean = settings.lenmean; wpool->lensd = settings.lensd; wpool->nsample = settings.nsample; wpool->nseq = 0; wpool->randomseq.resize(MAXABET); wpool->max_score = -FLT_MAX; float p1; P7Logoddsify(wpool->hmm, TRUE); P7DefaultNullModel(wpool->randomseq.data(), &p1); }
QList<UHMMSearchResult> UHMMSearch::search(plan7_s* _hmm, const char* seq, int seqLen, const UHMMSearchSettings& s, TaskStateInfo& si) { plan7_s * hmm = HMMIO::cloneHMM( _hmm ); //Set up optional Pfam score thresholds. threshold_s thresh; // contains all threshold (cutoff) info thresh.globE = s.globE; // use a reasonable Eval threshold thresh.globT = -FLT_MAX; // but no bit threshold thresh.domT = s.domT; // no domain bit threshold thresh.domE = s.domE; // and no domain Eval threshold thresh.autocut = CUT_NONE; // and no Pfam cutoffs used thresh.Z = s.eValueNSeqs; // Z not preset; use actual # of seqs int do_null2 = TRUE; // TRUE to adjust scores with null model #2 int do_forward = FALSE; // TRUE to use Forward() not Viterbi() int do_xnu = FALSE; // TRUE to filter sequences thru XNU QList<UHMMSearchResult> res; // the results of the method //get HMMERTaskLocalData HMMERTaskLocalData *tld = getHMMERTaskLocalData(); alphabet_s *al = &tld->al; SetAlphabet(hmm->atype); P7Logoddsify(hmm, !do_forward); //TODO: clone model to avoid changes in it or make it thread safe?? if (do_xnu && al->Alphabet_type == hmmNUCLEIC) { si.setError( "The HMM is a DNA model, and you can't use the --xnu filter on DNA data" ); return res; } /***************************************************************** * Set up optional Pfam score thresholds. * Can do this before starting any searches, since we'll only use 1 HMM. *****************************************************************/ if (!SetAutocuts(&thresh, hmm)) { si.setError( "HMM did not contain the GA, TC, or NC cutoffs you needed" ); return res; } // set up structures for storing output histogram_s *histogram = AllocHistogram(-200, 200, 100); //keeps full histogram of all scores tophit_s *ghit = AllocTophits(200); // per-seq hits: 200=lumpsize tophit_s *dhit = AllocTophits(200); // domain hits: 200=lumpsize int nseq = 0; // number of sequences searched #ifdef UGENE_CELL if( HMMSearchAlgo_CellOptimized == s.alg ) { if( hmm->M < MAX_HMM_LENGTH ) { main_loop_spe(hmm, seq, seqLen, &thresh, do_forward, do_null2, do_xnu, histogram, ghit, dhit, &nseq, si); } else { main_loop_serial(hmm, seq, seqLen, &thresh, do_forward, do_null2, do_xnu, histogram, ghit, dhit, &nseq, si); } } else #elif defined(HMMER_BUILD_WITH_SSE2) if( HMMSearchAlgo_SSEOptimized == s.alg ) { main_loop_opt(hmm, seq, seqLen, &thresh, do_forward, do_null2, do_xnu, histogram, ghit, dhit, &nseq, si, sseScoring); } else #endif if( HMMSearchAlgo_Conservative == s.alg ) { main_loop_serial(hmm, seq, seqLen, &thresh, do_forward, do_null2, do_xnu, histogram, ghit, dhit, &nseq, si); } else { assert( false && "bad hmmsearch algorithm selected" ); } // Process hit lists, produce text output // Set the theoretical EVD curve in our histogram using calibration in the HMM, if available. if (hmm->flags & PLAN7_STATS) { ExtremeValueSetHistogram(histogram, hmm->mu, hmm->lambda, histogram->lowscore, histogram->highscore, 0); } if (!thresh.Z) { thresh.Z = nseq; // set Z for good now that we're done } //report our output FullSortTophits(dhit); //int namewidth = MAX(8, TophitsMaxName(ghit)); // max width of sequence name // Report domain hits (sorted on E-value) for (int i = 0; i < dhit->num && !si.cancelFlag; i++) { float sc; // score of an HMM search double pvalue; // pvalue of an HMM score double evalue; // evalue of an HMM score char *name, *desc; // hit sequence name and description double motherp; // pvalue of a whole seq HMM score float mothersc; // score of a whole seq parent of domain int sqfrom, sqto; // coordinates in sequence int sqlen; // length of seq that was hit int hmmfrom, hmmto; // coordinate in HMM int ndom; // total # of domains in this seq int domidx; // number of this domain GetRankedHit(dhit, i, &pvalue, &sc, &motherp, &mothersc, &name, NULL, &desc, &sqfrom, &sqto, &sqlen, // seq position info &hmmfrom, &hmmto, NULL, // HMM position info &domidx, &ndom, // domain info NULL); // alignment info evalue = pvalue * (double) thresh.Z; if (motherp * (double) thresh.Z > thresh.globE || mothersc < thresh.globT) { continue; } else if (evalue <= thresh.domE && sc >= thresh.domT) { // hmm reports results in range [1...N] -> translate it to [0..N) res.append(UHMMSearchResult(U2Region(sqfrom-1, sqto-sqfrom+1), sc, evalue)); } } //Clean-up and exit. FreeHistogram(histogram); FreeTophits(ghit); FreeTophits(dhit); FreePlan7( hmm ); return res; }
static void main_loop_serial(struct plan7_s *hmm, int seed, int nsample, float lenmean, float lensd, int fixedlen, struct histogram_s **ret_hist, float *ret_max, int& cancelFlag, int& progress) { struct histogram_s *hist; struct dpmatrix_s *mx; float randomseq[MAXABET]; float p1; float max; char *seq; unsigned char *dsq; float score; int sqlen; int idx; // Initialize. // We assume we've already set the alphabet (safe, because // HMM input sets the alphabet). sre_srandom(seed); //get HMMERTaskLocalData HMMERTaskLocalData *tls = getHMMERTaskLocalData(); alphabet_s &al = tls->al; SetAlphabet(hmm->atype); P7Logoddsify(hmm, TRUE); P7DefaultNullModel(randomseq, &p1); hist = AllocHistogram(-200, 200, 100); mx = CreatePlan7Matrix(1, hmm->M, 25, 0); max = -FLT_MAX; progress = 0; int pStub; for (idx = 0; idx < nsample && !cancelFlag; idx++) { // choose length of random sequence if (fixedlen) { sqlen = fixedlen; } else { do sqlen = (int) Gaussrandom(lenmean, lensd); while (sqlen < 1); } // generate it seq = RandomSequence(al.Alphabet, randomseq, al.Alphabet_size, sqlen); dsq = DigitizeSequence(seq, sqlen); if (P7ViterbiSpaceOK(sqlen, hmm->M, mx)) { score = P7Viterbi(dsq, sqlen, hmm, mx, NULL); } else { score = P7SmallViterbi(dsq, sqlen, hmm, mx, NULL, pStub); } AddToHistogram(hist, score); max = qMax(score, max); progress = int(100*idx/float(nsample)); free(dsq); free(seq); } FreePlan7Matrix(mx); *ret_hist = hist; *ret_max = max; }
int main(void) { int master_tid; /* PVM TID of our master */ int slaveidx; /* my slave index (0..nslaves-1) */ struct plan7_s *hmm; /* HMM to calibrate, sent from master */ struct histogram_s *hist; /* score histogram */ int hmmidx; /* index of this HMM */ char *seq; /* synthetic random sequence */ char *dsq; /* digitized seq */ int len; /* length of seq */ float sc; /* score of seq aligned to HMM */ float max; /* maximum score seen in sample */ int seed; /* random number seed */ int nsample; /* number of seqs to sample */ int fixedlen; /* if nonzero, fixed length of seq */ float lenmean; /* Gaussian mean length of seq */ float lensd; /* Gaussian length std. dev. for seq */ int fitok; /* TRUE if EVD fit was OK */ float randomseq[MAXABET]; /* iid frequencies of residues */ float p1; int alphatype; /* alphabet type, hmmAMINO or hmmNUCLEIC */ int idx; int code; /* Register leave_pvm() cleanup function so any exit() call * first calls pvm_exit(). */ if (atexit(leave_pvm) != 0) { pvm_exit(); Die("slave couldn't register leave_pvm()"); } /***************************************************************** * initialization. * Master broadcasts the problem to us: parameters of the * HMM calibration. ******************************************************************/ master_tid = pvm_parent(); /* who's our master? */ pvm_recv(master_tid, HMMPVM_INIT); pvm_upkint(&nsample, 1, 1); pvm_upkint(&fixedlen, 1, 1); pvm_upkfloat(&lenmean, 1, 1); pvm_upkfloat(&lensd, 1, 1); /* tell the master we're OK and ready to go (or not) */ code = HMMPVM_OK; pvm_initsend(PvmDataDefault); pvm_pkint(&code, 1, 1); pvm_send(master_tid, HMMPVM_RESULTS); /***************************************************************** * Main loop. * Receive a random number seed, then an HMM to search against. * If we receive a -1 seed, we shut down. *****************************************************************/ slaveidx = -1; for (;;) { pvm_recv(master_tid, HMMPVM_WORK); pvm_upkint(&seed, 1, 1); if (seed == -1) break; /* shutdown signal */ pvm_upkint(&hmmidx, 1, 1); pvm_upkint(&alphatype,1, 1); SetAlphabet(alphatype); hmm = PVMUnpackHMM(); if (hmm == NULL) Die("oh no, the HMM never arrived"); if (slaveidx == -1) slaveidx = hmmidx; P7DefaultNullModel(randomseq, &p1); sre_srandom(seed); P7Logoddsify(hmm, TRUE); hist = AllocHistogram(-200, 200, 100); max = -FLT_MAX; for (idx = 0; idx < nsample; idx++) { /* choose length of random sequence */ if (fixedlen) len = fixedlen; else do len = (int) Gaussrandom(lenmean, lensd); while (len < 1); /* generate it */ seq = RandomSequence(Alphabet, randomseq, Alphabet_size, len); dsq = DigitizeSequence(seq, len); if (P7ViterbiSize(len, hmm->M) <= RAMLIMIT) sc = P7Viterbi(dsq, len, hmm, NULL); else sc = P7SmallViterbi(dsq, len, hmm, NULL); AddToHistogram(hist, sc); if (sc > max) max = sc; free(seq); free(dsq); } /* Fit an EVD to the observed histogram. * The TRUE left-censors and fits only the right slope of the histogram. * The 9999. is an arbitrary high number that means we won't trim outliers * on the right. */ fitok = ExtremeValueFitHistogram(hist, TRUE, 9999.); /* Return output to master. * Currently we don't send the histogram back, but we could. */ pvm_initsend(PvmDataDefault); pvm_pkint(&slaveidx, 1, 1); pvm_pkint(&hmmidx, 1, 1); PVMPackString(hmm->name); pvm_pkint(&fitok, 1, 1); pvm_pkfloat(&(hist->param[EVD_MU]), 1, 1); pvm_pkfloat(&(hist->param[EVD_LAMBDA]), 1, 1); pvm_pkfloat(&max, 1, 1); pvm_send(master_tid, HMMPVM_RESULTS); /* cleanup */ FreeHistogram(hist); FreePlan7(hmm); } /*********************************************** * Cleanup, return. ***********************************************/ return 0; /* pvm_exit() is called by atexit() registration. */ }
/* Function: main_loop_pvm() * Date: SRE, Wed Aug 19 13:59:54 1998 [St. Louis] * * Purpose: Given an HMM and parameters for synthesizing random * sequences; return a histogram of scores. * (PVM version) * * Args: hmm - an HMM to calibrate. * seed - random number seed * nsample - number of seqs to synthesize * lumpsize- # of seqs per slave exchange; controls granularity * lenmean - mean length of random sequence * lensd - std dev of random seq length * fixedlen- if nonzero, override lenmean, always this len * hist - RETURN: the score histogram * ret_max - RETURN: highest score seen in simulation * extrawatch - RETURN: total CPU time spend in slaves. * ret_nslaves- RETURN: number of PVM slaves run. * * Returns: (void) * hist is alloc'ed here, and must be free'd by caller. */ static void main_loop_pvm(struct plan7_s *hmm, int seed, int nsample, int lumpsize, float lenmean, float lensd, int fixedlen, struct histogram_s **ret_hist, float *ret_max, Stopwatch_t *extrawatch, int *ret_nslaves) { struct histogram_s *hist; int master_tid; int *slave_tid; int nslaves; int nsent; /* # of seqs we've asked for so far */ int ndone; /* # of seqs we've got results for so far */ int packet; /* # of seqs to have a slave do */ float max; int slaveidx; /* id of a slave */ float *sc; /* scores returned by a slave */ Stopwatch_t slavewatch; int i; StopwatchZero(extrawatch); hist = AllocHistogram(-200, 200, 100); max = -FLT_MAX; /* Initialize PVM */ if ((master_tid = pvm_mytid()) < 0) Die("pvmd not responding -- do you have PVM running?"); #if DEBUGLEVEL >= 1 pvm_catchout(stderr); /* catch output for debugging */ #endif PVMSpawnSlaves("hmmcalibrate-pvm", &slave_tid, &nslaves); /* Initialize the slaves */ pvm_initsend(PvmDataDefault); pvm_pkfloat(&lenmean, 1, 1); pvm_pkfloat(&lensd, 1, 1); pvm_pkint( &fixedlen, 1, 1); pvm_pkint( &Alphabet_type, 1, 1); pvm_pkint( &seed, 1, 1); if (! PVMPackHMM(hmm)) Die("Failed to pack the HMM"); pvm_mcast(slave_tid, nslaves, HMMPVM_INIT); SQD_DPRINTF1(("Initialized %d slaves\n", nslaves)); /* Confirm slaves' OK status. */ PVMConfirmSlaves(slave_tid, nslaves); SQD_DPRINTF1(("Slaves confirm that they're ok...\n")); /* Load the slaves */ nsent = ndone = 0; for (slaveidx = 0; slaveidx < nslaves; slaveidx++) { packet = (nsample - nsent > lumpsize ? lumpsize : nsample - nsent); pvm_initsend(PvmDataDefault); pvm_pkint(&packet, 1, 1); pvm_pkint(&slaveidx, 1, 1); pvm_send(slave_tid[slaveidx], HMMPVM_WORK); nsent += packet; } SQD_DPRINTF1(("Loaded %d slaves\n", nslaves)); /* Receive/send loop */ sc = MallocOrDie(sizeof(float) * lumpsize); while (nsent < nsample) { /* integrity check of slaves */ PVMCheckSlaves(slave_tid, nslaves); /* receive results */ SQD_DPRINTF2(("Waiting for results...\n")); pvm_recv(-1, HMMPVM_RESULTS); pvm_upkint(&slaveidx, 1, 1); pvm_upkint(&packet, 1, 1); pvm_upkfloat(sc, packet, 1); SQD_DPRINTF2(("Got results.\n")); ndone += packet; /* store results */ for (i = 0; i < packet; i++) { AddToHistogram(hist, sc[i]); if (sc[i] > max) max = sc[i]; } /* send new work */ packet = (nsample - nsent > lumpsize ? lumpsize : nsample - nsent); pvm_initsend(PvmDataDefault); pvm_pkint(&packet, 1, 1); pvm_pkint(&slaveidx, 1, 1); pvm_send(slave_tid[slaveidx], HMMPVM_WORK); SQD_DPRINTF2(("Told slave %d to do %d more seqs.\n", slaveidx, packet)); nsent += packet; } /* Wait for the last output to come in. */ while (ndone < nsample) { /* integrity check of slaves */ PVMCheckSlaves(slave_tid, nslaves); /* receive results */ SQD_DPRINTF1(("Waiting for final results...\n")); pvm_recv(-1, HMMPVM_RESULTS); pvm_upkint(&slaveidx, 1, 1); pvm_upkint(&packet, 1, 1); pvm_upkfloat(sc, packet, 1); SQD_DPRINTF2(("Got some final results.\n")); ndone += packet; /* store results */ for (i = 0; i < packet; i++) { AddToHistogram(hist, sc[i]); if (sc[i] > max) max = sc[i]; } } /* Shut down the slaves: send -1,-1,-1. */ pvm_initsend(PvmDataDefault); packet = -1; pvm_pkint(&packet, 1, 1); pvm_pkint(&packet, 1, 1); pvm_pkint(&packet, 1, 1); pvm_mcast(slave_tid, nslaves, HMMPVM_WORK); /* Collect stopwatch results; quit the VM; return. */ for (i = 0; i < nslaves; i++) { pvm_recv(-1, HMMPVM_RESULTS); pvm_upkint(&slaveidx, 1, 1); StopwatchPVMUnpack(&slavewatch); SQD_DPRINTF1(("Slave %d finished; says it used %.2f cpu, %.2f sys\n", slaveidx, slavewatch.user, slavewatch.sys)); StopwatchInclude(extrawatch, &slavewatch); } free(slave_tid); free(sc); pvm_exit(); *ret_hist = hist; *ret_max = max; *ret_nslaves = nslaves; return; }