void FreeCharCub( char ***cub )
{
int i;
for( i=0; cub[i]; i++ )
{
FreeCharMtx( cub[i] );
}
free( cub );
}
void foldrna( int nseq1, int nseq2, char **seq1, char **seq2, double *eff1, double *eff2, RNApair ***grouprna1, RNApair ***grouprna2, float **impmtx, int *gapmap1, int *gapmap2, RNApair *additionalpair )
{
int i, j;
// int ui, uj;
// int uiup, ujup;
int uido, ujdo;
static TLS char **useq1, **useq2;
static TLS char **oseq1, **oseq2, **oseq1r, **oseq2r, *odir1, *odir2;
static TLS RNApair **pairprob1, **pairprob2;
static TLS RNApair *pairpt1, *pairpt2;
int lgth1 = strlen( seq1[0] );
int lgth2 = strlen( seq2[0] );
static TLS float **impmtx2;
static TLS float **map;
// double lenfac;
float prob;
int **sgapmap1, **sgapmap2;
char *nogapdum;
float **tbppmtx;
// fprintf( stderr, "nseq1=%d, lgth1=%d\n", nseq1, lgth1 );
useq1 = AllocateCharMtx( nseq1, lgth1+10 );
useq2 = AllocateCharMtx( nseq2, lgth2+10 );
oseq1 = AllocateCharMtx( nseq1, lgth1+10 );
oseq2 = AllocateCharMtx( nseq2, lgth2+10 );
oseq1r = AllocateCharMtx( nseq1, lgth1+10 );
oseq2r = AllocateCharMtx( nseq2, lgth2+10 );
odir1 = AllocateCharVec( lgth1+10 );
odir2 = AllocateCharVec( lgth2+10 );
sgapmap1 = AllocateIntMtx( nseq1, lgth1+1 );
sgapmap2 = AllocateIntMtx( nseq2, lgth2+1 );
nogapdum = AllocateCharVec( MAX( lgth1, lgth2 ) );
pairprob1 = (RNApair **)calloc( lgth1, sizeof( RNApair *) );
pairprob2 = (RNApair **)calloc( lgth2, sizeof( RNApair *) );
map = AllocateFloatMtx( lgth1, lgth2 );
impmtx2 = AllocateFloatMtx( lgth1, lgth2 );
tbppmtx = AllocateFloatMtx( lgth1, lgth2 );
for( i=0; i<nseq1; i++ ) strcpy( useq1[i], seq1[i] );
for( i=0; i<nseq2; i++ ) strcpy( useq2[i], seq2[i] );
for( i=0; i<nseq1; i++ ) strcpy( oseq1[i], seq1[i] );
for( i=0; i<nseq2; i++ ) strcpy( oseq2[i], seq2[i] );
for( i=0; i<nseq1; i++ ) commongappick_record( 1, useq1+i, sgapmap1[i] );
for( i=0; i<nseq2; i++ ) commongappick_record( 1, useq2+i, sgapmap2[i] );
for( i=0; i<lgth1; i++ )
{
pairprob1[i] = (RNApair *)calloc( 1, sizeof( RNApair ) );
pairprob1[i][0].bestpos = -1;
pairprob1[i][0].bestscore = -1;
}
for( i=0; i<lgth2; i++ )
{
pairprob2[i] = (RNApair *)calloc( 1, sizeof( RNApair ) );
pairprob2[i][0].bestpos = -1;
pairprob2[i][0].bestscore = -1;
}
utot( nseq1, lgth1, oseq1 );
utot( nseq2, lgth2, oseq2 );
// fprintf( stderr, "folding group1\n" );
// rnalocal( oseq1, useq1, eff1, eff1, nseq1, nseq1, lgth1+10, pair1 );
/* base-pairing probability of group 1 */
if( rnaprediction == 'r' )
rnaalifoldcall( oseq1, nseq1, pairprob1 );
else
mccaskillextract( oseq1, useq1, nseq1, pairprob1, grouprna1, sgapmap1, eff1 );
// fprintf( stderr, "folding group2\n" );
// rnalocal( oseq2, useq2, eff2, eff2, nseq2, nseq2, lgth2+10, pair2 );
/* base-pairing probability of group 2 */
if( rnaprediction == 'r' )
rnaalifoldcall( oseq2, nseq2, pairprob2 );
else
mccaskillextract( oseq2, useq2, nseq2, pairprob2, grouprna2, sgapmap2, eff2 );
#if 0
makerseq( oseq1, oseq1r, odir1, pairprob1, nseq1, lgth1 );
makerseq( oseq2, oseq2r, odir2, pairprob2, nseq2, lgth2 );
fprintf( stderr, "%s\n", odir2 );
for( i=0; i<nseq1; i++ )
{
fprintf( stdout, ">ori\n%s\n", oseq1[0] );
fprintf( stdout, ">rev\n%s\n", oseq1r[0] );
}
#endif
/* similarity score */
Lalignmm_hmout( oseq1, oseq2, eff1, eff2, nseq1, nseq2, 10000, NULL, NULL, NULL, NULL, map );
if( 1 )
{
if( RNAscoremtx == 'n' )
{
for( i=0; i<lgth1; i++ ) for( j=0; j<lgth2; j++ )
{
// impmtx2[i][j] = osoiaveragescore( nseq1, nseq2, oseq1, oseq2, eff1, eff2, i, j ) * consweight_multi;
impmtx2[i][j] = 0.0;
}
}
else if( RNAscoremtx == 'r' )
{
for( i=0; i<lgth1; i++ ) for( j=0; j<lgth2; j++ )
{
tbppmtx[i][j] = 1.0;
impmtx2[i][j] = 0.0;
}
for( i=0; i<lgth1; i++ ) for( pairpt1=pairprob1[i]; pairpt1->bestpos!=-1; pairpt1++ )
{
for( j=0; j<lgth2; j++ ) for( pairpt2=pairprob2[j]; pairpt2->bestpos!=-1; pairpt2++ )
{
uido = pairpt1->bestpos;
ujdo = pairpt2->bestpos;
prob = pairpt1->bestscore * pairpt2->bestscore;
if( uido > -1 && ujdo > -1 )
{
if( uido > i && j > ujdo )
{
impmtx2[i][j] += prob * pairedribosumscore53( nseq1, nseq2, oseq1, oseq2, eff1, eff2, i, j, uido, ujdo ) * consweight_multi;
tbppmtx[i][j] -= prob;
}
else if( i < uido && j < ujdo )
{
impmtx2[i][j] += prob * pairedribosumscore35( nseq1, nseq2, oseq1, oseq2, eff1, eff2, i, j, uido, ujdo ) * consweight_multi;
tbppmtx[i][j] -= prob;
}
}
}
}
for( i=0; i<lgth1; i++ )
{
for( j=0; j<lgth2; j++ )
{
impmtx2[i][j] += tbppmtx[i][j] * singleribosumscore( nseq1, nseq2, oseq1, oseq2, eff1, eff2, i, j ) * consweight_multi;
}
}
}
/* four-way consistency */
for( i=0; i<lgth1; i++ ) for( pairpt1=pairprob1[i]; pairpt1->bestpos!=-1; pairpt1++ )
{
// if( pairprob1[i] == NULL ) continue;
for( j=0; j<lgth2; j++ ) for( pairpt2=pairprob2[j]; pairpt2->bestpos!=-1; pairpt2++ )
{
// fprintf( stderr, "i=%d, j=%d, pn1=%d, pn2=%d\n", i, j, pairpt1-pairprob1[i], pairpt2-pairprob2[j] );
// if( pairprob2[j] == NULL ) continue;
uido = pairpt1->bestpos;
ujdo = pairpt2->bestpos;
prob = pairpt1->bestscore * pairpt2->bestscore;
// prob = 1.0;
// fprintf( stderr, "i=%d->uido=%d, j=%d->ujdo=%d\n", i, uido, j, ujdo );
// fprintf( stderr, "impmtx2[%d][%d] = %f\n", i, j, impmtx2[i][j] );
// if( i < uido && j > ujdo ) continue;
// if( i > uido && j < ujdo ) continue;
// posdistj = abs( ujdo-j );
// if( uido > -1 && ujdo > -1 )
if( uido > -1 && ujdo > -1 && ( ( i > uido && j > ujdo ) || ( i < uido && j < ujdo ) ) )
{
{
impmtx2[i][j] += MAX( 0, map[uido][ujdo] ) * consweight_rna * 600 * prob; // osoi
}
}
}
}
for( i=0; i<lgth1; i++ ) for( j=0; j<lgth2; j++ )
{
impmtx[i][j] += impmtx2[i][j];
// fprintf( stderr, "fastathreshold=%f, consweight_multi=%f, consweight_rna=%f\n", fastathreshold, consweight_multi, consweight_rna );
// impmtx[i][j] *= 0.5;
}
// impmtx[0][0] += 10000.0;
// impmtx[lgth1-1][lgth2-1] += 10000.0;
#if 0
fprintf( stdout, "#impmtx2 = \n" );
for( i=0; i<lgth1; i++ )
{
for( j=0; j<lgth2; j++ )
{
fprintf( stdout, "%d %d %f\n", i, j, impmtx2[i][j] );
}
fprintf( stdout, "\n" );
}
exit( 1 );
#endif
}
FreeCharMtx( useq1 );
FreeCharMtx( useq2 );
FreeCharMtx( oseq1 );
FreeCharMtx( oseq2 );
FreeCharMtx( oseq1r );
FreeCharMtx( oseq2r );
free( odir1 );
free( odir2 );
FreeFloatMtx( impmtx2 );
FreeFloatMtx( map );
FreeIntMtx( sgapmap1 );
FreeIntMtx( sgapmap2 );
FreeFloatMtx( tbppmtx );
for( i=0; i<lgth1; i++ ) free( pairprob1[i] );
for( i=0; i<lgth2; i++ ) free( pairprob2[i] );
free( pairprob1 );
free( pairprob2 );
}
int main( int argc, char *argv[] )
{
FILE *infp;
FILE *difp;
int nlenmin;
char **name;
char **seq;
char *tmpseq;
char line[100];
int *nlen;
int i;
arguments( argc, argv );
if( inputfile )
{
infp = fopen( inputfile, "r" );
if( !infp )
{
fprintf( stderr, "Cannot open %s\n", inputfile );
exit( 1 );
}
}
else
infp = stdin;
if( directionfile )
{
difp = fopen( directionfile, "r" );
if( !difp )
{
fprintf( stderr, "Cannot open %s\n", directionfile );
exit( 1 );
}
}
else
{
fprintf( stderr, "Give directionfile!\n" );
}
dorp = NOTSPECIFIED;
getnumlen_casepreserve( infp, &nlenmin );
fprintf( stderr, "%d x %d - %d %c\n", njob, nlenmax, nlenmin, dorp );
seq = AllocateCharMtx( njob, nlenmax+1 );
tmpseq = AllocateCharVec( MAX( B, nlenmax )+1 );
name = AllocateCharMtx( njob, B+1 );
nlen = AllocateIntVec( njob );
readData_pointer_casepreserve( infp, name, nlen, seq );
for( i=0; i<njob; i++ )
{
fgets( line, 99, difp );
if( line[0] != '_' )
{
fprintf( stderr, "Format error!\n" );
exit( 1 );
}
if( line[1] == 'R' )
{
sreverse( tmpseq, seq[i] );
strcpy( seq[i], tmpseq );
strncpy( tmpseq, name[i]+1, B-3 );
tmpseq[B-3] = 0;
strcpy( name[i]+1, "_R_" );
strcpy( name[i]+4, tmpseq );
}
else if( line[1] == 'F' )
{
;
}
else
{
fprintf( stderr, "Format error!\n" );
exit( 1 );
}
}
for( i=0; i<njob; i++ )
{
fprintf( stdout, ">%s\n", name[i]+1 );
fprintf( stdout, "%s\n", seq[i] );
}
free( nlen );
FreeCharMtx( seq );
FreeCharMtx( name );
free( tmpseq );
return( 0 );
}
float genL__align11( char **seq1, char **seq2, int alloclen, int *off1pt, int *off2pt )
/* score no keisan no sai motokaraaru gap no atukai ni mondai ga aru */
{
// int k;
register int i, j;
int lasti, lastj;
int lgth1, lgth2;
int resultlen;
float wm = 0.0; /* int ?????? */
float g;
float *currentw, *previousw;
#if 1
float *wtmp;
int *ijpipt;
int *ijpjpt;
float *mjpt, *Mjpt, *prept, *curpt;
int *mpjpt, *Mpjpt;
#endif
static TLS float mi, *m;
static TLS float Mi, *largeM;
static TLS int **ijpi;
static TLS int **ijpj;
static TLS int mpi, *mp;
static TLS int Mpi, *Mp;
static TLS float *w1, *w2;
static TLS float *match;
static TLS float *initverticalw; /* kufuu sureba iranai */
static TLS float *lastverticalw; /* kufuu sureba iranai */
static TLS char **mseq1;
static TLS char **mseq2;
static TLS char **mseq;
static TLS float **cpmx1;
static TLS float **cpmx2;
static TLS int **intwork;
static TLS float **floatwork;
static TLS int orlgth1 = 0, orlgth2 = 0;
float maxwm;
float tbk;
int tbki, tbkj;
int endali, endalj;
// float localthr = 0.0;
// float localthr2 = 0.0;
float fpenalty = (float)penalty;
float fpenalty_OP = (float)penalty_OP;
float fpenalty_ex = (float)penalty_ex;
// float fpenalty_EX = (float)penalty_EX;
float foffset = (float)offset;
float localthr = -foffset;
float localthr2 = -foffset;
if( seq1 == NULL )
{
if( orlgth1 > 0 && orlgth2 > 0 )
{
orlgth1 = 0;
orlgth2 = 0;
free( mseq1 );
free( mseq2 );
FreeFloatVec( w1 );
FreeFloatVec( w2 );
FreeFloatVec( match );
FreeFloatVec( initverticalw );
FreeFloatVec( lastverticalw );
FreeFloatVec( m );
FreeIntVec( mp );
free( largeM );
free( Mp );
FreeCharMtx( mseq );
FreeFloatMtx( cpmx1 );
FreeFloatMtx( cpmx2 );
FreeFloatMtx( floatwork );
FreeIntMtx( intwork );
}
return( 0.0 );
}
// fprintf( stderr, "@@@@@@@@@@@@@ penalty_OP = %f, penalty_EX = %f, pelanty = %f\n", fpenalty_OP, fpenalty_EX, fpenalty );
if( orlgth1 == 0 )
{
mseq1 = AllocateCharMtx( njob, 0 );
mseq2 = AllocateCharMtx( njob, 0 );
}
lgth1 = strlen( seq1[0] );
lgth2 = strlen( seq2[0] );
if( lgth1 > orlgth1 || lgth2 > orlgth2 )
{
int ll1, ll2;
if( orlgth1 > 0 && orlgth2 > 0 )
{
FreeFloatVec( w1 );
FreeFloatVec( w2 );
FreeFloatVec( match );
FreeFloatVec( initverticalw );
FreeFloatVec( lastverticalw );
FreeFloatVec( m );
FreeIntVec( mp );
FreeFloatVec( largeM );
FreeIntVec( Mp );
FreeCharMtx( mseq );
FreeFloatMtx( cpmx1 );
FreeFloatMtx( cpmx2 );
FreeFloatMtx( floatwork );
FreeIntMtx( intwork );
}
ll1 = MAX( (int)(1.3*lgth1), orlgth1 ) + 100;
ll2 = MAX( (int)(1.3*lgth2), orlgth2 ) + 100;
#if DEBUG
fprintf( stderr, "\ntrying to allocate (%d+%d)xn matrices ... ", ll1, ll2 );
#endif
w1 = AllocateFloatVec( ll2+2 );
w2 = AllocateFloatVec( ll2+2 );
match = AllocateFloatVec( ll2+2 );
initverticalw = AllocateFloatVec( ll1+2 );
lastverticalw = AllocateFloatVec( ll1+2 );
m = AllocateFloatVec( ll2+2 );
mp = AllocateIntVec( ll2+2 );
largeM = AllocateFloatVec( ll2+2 );
Mp = AllocateIntVec( ll2+2 );
mseq = AllocateCharMtx( njob, ll1+ll2 );
cpmx1 = AllocateFloatMtx( nalphabets, ll1+2 );
cpmx2 = AllocateFloatMtx( nalphabets, ll2+2 );
floatwork = AllocateFloatMtx( nalphabets, MAX( ll1, ll2 )+2 );
intwork = AllocateIntMtx( nalphabets, MAX( ll1, ll2 )+2 );
#if DEBUG
fprintf( stderr, "succeeded\n" );
#endif
orlgth1 = ll1 - 100;
orlgth2 = ll2 - 100;
}
mseq1[0] = mseq[0];
mseq2[0] = mseq[1];
if( orlgth1 > commonAlloc1 || orlgth2 > commonAlloc2 )
{
int ll1, ll2;
if( commonAlloc1 && commonAlloc2 )
{
FreeIntMtx( commonIP );
FreeIntMtx( commonJP );
}
ll1 = MAX( orlgth1, commonAlloc1 );
ll2 = MAX( orlgth2, commonAlloc2 );
#if DEBUG
fprintf( stderr, "\n\ntrying to allocate %dx%d matrices ... ", ll1+1, ll2+1 );
#endif
commonIP = AllocateIntMtx( ll1+10, ll2+10 );
commonJP = AllocateIntMtx( ll1+10, ll2+10 );
#if DEBUG
fprintf( stderr, "succeeded\n\n" );
#endif
commonAlloc1 = ll1;
commonAlloc2 = ll2;
}
ijpi = commonIP;
ijpj = commonJP;
#if 0
for( i=0; i<lgth1; i++ )
fprintf( stderr, "ogcp1[%d]=%f\n", i, ogcp1[i] );
#endif
currentw = w1;
previousw = w2;
match_calc( initverticalw, seq2, seq1, 0, lgth1 );
match_calc( currentw, seq1, seq2, 0, lgth2 );
lasti = lgth2+1;
for( j=1; j<lasti; ++j )
{
m[j] = currentw[j-1]; mp[j] = 0;
largeM[j] = currentw[j-1]; Mp[j] = 0;
}
lastverticalw[0] = currentw[lgth2-1];
#if 0
fprintf( stderr, "currentw = \n" );
for( i=0; i<lgth1+1; i++ )
{
fprintf( stderr, "%5.2f ", currentw[i] );
}
fprintf( stderr, "\n" );
fprintf( stderr, "initverticalw = \n" );
for( i=0; i<lgth2+1; i++ )
{
fprintf( stderr, "%5.2f ", initverticalw[i] );
}
fprintf( stderr, "\n" );
#endif
#if DEBUG2
fprintf( stderr, "\n" );
fprintf( stderr, " " );
for( j=0; j<lgth2+1; j++ )
fprintf( stderr, "%c ", seq2[0][j] );
fprintf( stderr, "\n" );
#endif
localstop = lgth1+lgth2+1;
maxwm = -999999999.9;
endali = endalj = 0;
#if DEBUG2
fprintf( stderr, "\n" );
fprintf( stderr, "%c ", seq1[0][0] );
for( j=0; j<lgth2+1; j++ )
fprintf( stderr, "%5.0f ", currentw[j] );
fprintf( stderr, "\n" );
#endif
lasti = lgth1+1;
for( i=1; i<lasti; i++ )
{
wtmp = previousw;
previousw = currentw;
currentw = wtmp;
previousw[0] = initverticalw[i-1];
match_calc( currentw, seq1, seq2, i, lgth2 );
#if DEBUG2
fprintf( stderr, "%c ", seq1[0][i] );
fprintf( stderr, "%5.0f ", currentw[0] );
#endif
#if XXXXXXX
fprintf( stderr, "\n" );
fprintf( stderr, "i=%d\n", i );
fprintf( stderr, "currentw = \n" );
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "%5.2f ", currentw[j] );
}
fprintf( stderr, "\n" );
#endif
#if XXXXXXX
fprintf( stderr, "\n" );
fprintf( stderr, "i=%d\n", i );
fprintf( stderr, "currentw = \n" );
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "%5.2f ", currentw[j] );
}
fprintf( stderr, "\n" );
#endif
currentw[0] = initverticalw[i];
mi = previousw[0]; mpi = 0;
Mi = previousw[0]; Mpi = 0;
#if 0
if( mi < localthr ) mi = localthr2;
#endif
ijpipt = ijpi[i] + 1;
ijpjpt = ijpj[i] + 1;
mjpt = m + 1;
Mjpt = largeM + 1;
prept = previousw;
curpt = currentw + 1;
mpjpt = mp + 1;
Mpjpt = Mp + 1;
tbk = -999999.9;
tbki = 0;
tbkj = 0;
lastj = lgth2+1;
for( j=1; j<lastj; j++ )
{
wm = *prept;
*ijpipt = i-1;
*ijpjpt = j-1;
// fprintf( stderr, "i,j=%d,%d %c-%c\n", i, j, seq1[0][i], seq2[0][j] );
// fprintf( stderr, "wm=%f\n", wm );
#if 0
fprintf( stderr, "%5.0f->", wm );
#endif
g = mi + fpenalty;
#if 0
fprintf( stderr, "%5.0f?", g );
#endif
if( g > wm )
{
wm = g;
// *ijpipt = i - 1;
*ijpjpt = mpi;
}
g = *prept;
if( g > mi )
{
mi = g;
mpi = j-1;
}
#if USE_PENALTY_EX
mi += fpenalty_ex;
#endif
#if 0
fprintf( stderr, "%5.0f->", wm );
#endif
g = *mjpt + fpenalty;
#if 0
fprintf( stderr, "m%5.0f?", g );
#endif
if( g > wm )
{
wm = g;
*ijpipt = *mpjpt;
*ijpjpt = j - 1; //IRU!
}
g = *prept;
if( g > *mjpt )
{
*mjpt = g;
*mpjpt = i-1;
}
#if USE_PENALTY_EX
*mjpt += fpenalty_ex;
#endif
g = tbk + fpenalty_OP;
// g = tbk;
if( g > wm )
{
wm = g;
*ijpipt = tbki;
*ijpjpt = tbkj;
// fprintf( stderr, "hit! i%d, j%d, ijpi = %d, ijpj = %d\n", i, j, *ijpipt, *ijpjpt );
}
// g = Mi;
if( Mi > tbk )
{
tbk = Mi; //error desu.
tbki = i-1;
tbkj = Mpi;
}
// g = *Mjpt;
if( *Mjpt > tbk )
{
tbk = *Mjpt;
tbki = *Mpjpt;
tbkj = j-1;
}
// tbk += fpenalty_EX;// + foffset;
// g = *prept;
if( *prept > *Mjpt )
{
*Mjpt = *prept;
*Mpjpt = i-1;
}
// *Mjpt += fpenalty_EX;// + foffset;
// g = *prept;
if( *prept > Mi )
{
Mi = *prept;
Mpi = j-1;
}
// Mi += fpenalty_EX;// + foffset;
// fprintf( stderr, "wm=%f, tbk=%f(%c-%c), mi=%f, *mjpt=%f\n", wm, tbk, seq1[0][tbki], seq2[0][tbkj], mi, *mjpt );
// fprintf( stderr, "ijp = %c,%c\n", seq1[0][abs(*ijpipt)], seq2[0][abs(*ijpjpt)] );
if( maxwm < wm )
{
maxwm = wm;
endali = i;
endalj = j;
}
#if 1
if( wm < localthr )
{
// fprintf( stderr, "stop i=%d, j=%d, curpt=%f\n", i, j, *curpt );
*ijpipt = localstop;
// *ijpjpt = localstop;
wm = localthr2;
}
#endif
#if 0
fprintf( stderr, "%5.0f ", *curpt );
#endif
#if DEBUG2
fprintf( stderr, "%5.0f ", wm );
// fprintf( stderr, "%c-%c *ijppt = %d, localstop = %d\n", seq1[0][i], seq2[0][j], *ijppt, localstop );
#endif
*curpt += wm;
ijpipt++;
ijpjpt++;
mjpt++;
Mjpt++;
prept++;
mpjpt++;
Mpjpt++;
curpt++;
}
#if DEBUG2
fprintf( stderr, "\n" );
#endif
lastverticalw[i] = currentw[lgth2-1];
}
#if DEBUG2
fprintf( stderr, "maxwm = %f\n", maxwm );
fprintf( stderr, "endali = %d\n", endali );
fprintf( stderr, "endalj = %d\n", endalj );
#endif
if( ijpi[endali][endalj] == localstop ) // && ijpj[endali][endalj] == localstop )
{
strcpy( seq1[0], "" );
strcpy( seq2[0], "" );
*off1pt = *off2pt = 0;
return( 0.0 );
}
gentracking( currentw, lastverticalw, seq1, seq2, mseq1, mseq2, cpmx1, cpmx2, ijpi, ijpj, off1pt, off2pt, endali, endalj );
// fprintf( stderr, "### impmatch = %f\n", *impmatch );
resultlen = strlen( mseq1[0] );
if( alloclen < resultlen || resultlen > N )
{
fprintf( stderr, "alloclen=%d, resultlen=%d, N=%d\n", alloclen, resultlen, N );
ErrorExit( "LENGTH OVER!\n" );
}
strcpy( seq1[0], mseq1[0] );
strcpy( seq2[0], mseq2[0] );
#if 0
fprintf( stderr, "\n" );
fprintf( stderr, ">\n%s\n", mseq1[0] );
fprintf( stderr, ">\n%s\n", mseq2[0] );
#endif
return( maxwm );
}
int main( int argc, char *argv[] )
{
FILE *infp;
int nlenmin;
char **name;
char **seq;
int *nlen;
int i;
char *usual;
arguments( argc, argv );
if( inputfile )
{
infp = fopen( inputfile, "r" );
if( !infp )
{
fprintf( stderr, "Cannot open %s\n", inputfile );
exit( 1 );
}
}
else
infp = stdin;
// dorp = NOTSPECIFIED;
getnumlen_casepreserve( infp, &nlenmin );
fprintf( stderr, "%d x %d - %d %c\n", njob, nlenmax, nlenmin, dorp );
seq = AllocateCharMtx( njob, nlenmax+1 );
name = AllocateCharMtx( njob, B+1 );
nlen = AllocateIntVec( njob );
readData_pointer_casepreserve( infp, name, nlen, seq );
// for( i=0; i<njob; i++ ) gappick_samestring( seq[i] );
#if 0
FILE *origfp;
origfp = fopen( "_original", "w" );
if( !origfp )
{
fprintf( stderr, "Cannot open _original\n" );
exit( 1 );
}
for( i=0; i<njob; i++ )
{
nlen[i] = strlen( seq[i] );
fprintf( origfp, ">%s\n", name[i]+1 );
if( seq[i][nlen[i]-1] == '\n' ) seq[i][nlen[i]-1] = 0;
fprintf( origfp, "%s\n", seq[i] );
}
fclose( origfp );
#endif
if( dorp == 'p' )
{
usual = "ARNDCQEGHILKMFPSTWYVarndcqeghilkmfpstwyv-.";
replace_unusual( njob, seq, usual, 'X', toupper );
}
else
{
usual = "ATGCUatgcuBDHKMNRSVWYXbdhkmnrsvwyx-";
replace_unusual( njob, seq, usual, 'n', tolower );
}
for( i=0; i<njob; i++ )
{
fprintf( stdout, ">_os_%d_oe_%s\n", i+seedoffset, name[i]+1 );
fprintf( stdout, "%s\n", seq[i] );
}
free( nlen );
FreeCharMtx( seq );
FreeCharMtx( name );
return( 0 );
}
float G__align11( char **seq1, char **seq2, int alloclen )
/* score no keisan no sai motokaraaru gap no atukai ni mondai ga aru */
{
// int k;
register int i, j;
int lasti; /* outgap == 0 -> lgth1, outgap == 1 -> lgth1+1 */
int lgth1, lgth2;
int resultlen;
float wm; /* int ?????? */
float g;
float *currentw, *previousw;
float fpenalty = (float)penalty;
#if USE_PENALTY_EX
float fpenalty_ex = (float)penalty_ex;
#endif
#if 1
float *wtmp;
int *ijppt;
float *mjpt, *prept, *curpt;
int *mpjpt;
#endif
static float mi, *m;
static int **ijp;
static int mpi, *mp;
static float *w1, *w2;
static float *match;
static float *initverticalw; /* kufuu sureba iranai */
static float *lastverticalw; /* kufuu sureba iranai */
static char **mseq1;
static char **mseq2;
static char **mseq;
static int **intwork;
static float **floatwork;
static int orlgth1 = 0, orlgth2 = 0;
wm = 0.0;
if( orlgth1 == 0 )
{
mseq1 = AllocateCharMtx( njob, 0 );
mseq2 = AllocateCharMtx( njob, 0 );
}
lgth1 = strlen( seq1[0] );
lgth2 = strlen( seq2[0] );
if( lgth1 <= 0 || lgth2 <= 0 )
{
fprintf( stderr, "WARNING (g11): lgth1=%d, lgth2=%d\n", lgth1, lgth2 );
}
if( lgth1 > orlgth1 || lgth2 > orlgth2 )
{
int ll1, ll2;
if( orlgth1 > 0 && orlgth2 > 0 )
{
FreeFloatVec( w1 );
FreeFloatVec( w2 );
FreeFloatVec( match );
FreeFloatVec( initverticalw );
FreeFloatVec( lastverticalw );
FreeFloatVec( m );
FreeIntVec( mp );
FreeCharMtx( mseq );
FreeFloatMtx( floatwork );
FreeIntMtx( intwork );
}
ll1 = MAX( (int)(1.3*lgth1), orlgth1 ) + 100;
ll2 = MAX( (int)(1.3*lgth2), orlgth2 ) + 100;
#if DEBUG
fprintf( stderr, "\ntrying to allocate (%d+%d)xn matrices ... ", ll1, ll2 );
#endif
w1 = AllocateFloatVec( ll2+2 );
w2 = AllocateFloatVec( ll2+2 );
match = AllocateFloatVec( ll2+2 );
initverticalw = AllocateFloatVec( ll1+2 );
lastverticalw = AllocateFloatVec( ll1+2 );
m = AllocateFloatVec( ll2+2 );
mp = AllocateIntVec( ll2+2 );
mseq = AllocateCharMtx( njob, ll1+ll2 );
floatwork = AllocateFloatMtx( 26, MAX( ll1, ll2 )+2 );
intwork = AllocateIntMtx( 26, MAX( ll1, ll2 )+2 );
#if DEBUG
fprintf( stderr, "succeeded\n" );
#endif
orlgth1 = ll1 - 100;
orlgth2 = ll2 - 100;
}
mseq1[0] = mseq[0];
mseq2[0] = mseq[1];
if( orlgth1 > commonAlloc1 || orlgth2 > commonAlloc2 )
{
int ll1, ll2;
if( commonAlloc1 && commonAlloc2 )
{
FreeIntMtx( commonIP );
}
ll1 = MAX( orlgth1, commonAlloc1 );
ll2 = MAX( orlgth2, commonAlloc2 );
#if DEBUG
fprintf( stderr, "\n\ntrying to allocate %dx%d matrices ... ", ll1+1, ll2+1 );
#endif
commonIP = AllocateIntMtx( ll1+10, ll2+10 );
#if DEBUG
fprintf( stderr, "succeeded\n\n" );
#endif
commonAlloc1 = ll1;
commonAlloc2 = ll2;
}
ijp = commonIP;
#if 0
for( i=0; i<lgth1; i++ )
fprintf( stderr, "ogcp1[%d]=%f\n", i, ogcp1[i] );
#endif
currentw = w1;
previousw = w2;
match_calc( initverticalw, seq2, seq1, 0, lgth1 );
match_calc( currentw, seq1, seq2, 0, lgth2 );
if( outgap == 1 )
{
for( i=1; i<lgth1+1; i++ )
{
initverticalw[i] += fpenalty;
}
for( j=1; j<lgth2+1; j++ )
{
currentw[j] += fpenalty;
}
}
for( j=1; j<lgth2+1; ++j )
{
m[j] = currentw[j-1]; mp[j] = 0;
}
if( lgth2 == 0 )
lastverticalw[0] = 0.0; // lgth2==0 no toki error
else
lastverticalw[0] = currentw[lgth2-1]; // lgth2==0 no toki error
if( outgap ) lasti = lgth1+1; else lasti = lgth1;
#if XXXXXXX
fprintf( stderr, "currentw = \n" );
for( i=0; i<lgth1+1; i++ )
{
fprintf( stderr, "%5.2f ", currentw[i] );
}
fprintf( stderr, "\n" );
fprintf( stderr, "initverticalw = \n" );
for( i=0; i<lgth2+1; i++ )
{
fprintf( stderr, "%5.2f ", initverticalw[i] );
}
fprintf( stderr, "\n" );
#endif
for( i=1; i<lasti; i++ )
{
wtmp = previousw;
previousw = currentw;
currentw = wtmp;
previousw[0] = initverticalw[i-1];
match_calc( currentw, seq1, seq2, i, lgth2 );
#if XXXXXXX
fprintf( stderr, "\n" );
fprintf( stderr, "i=%d\n", i );
fprintf( stderr, "currentw = \n" );
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "%5.2f ", currentw[j] );
}
fprintf( stderr, "\n" );
#endif
#if XXXXXXX
fprintf( stderr, "\n" );
fprintf( stderr, "i=%d\n", i );
fprintf( stderr, "currentw = \n" );
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "%5.2f ", currentw[j] );
}
fprintf( stderr, "\n" );
#endif
currentw[0] = initverticalw[i];
mi = previousw[0]; mpi = 0;
ijppt = ijp[i] + 1;
mjpt = m + 1;
prept = previousw;
curpt = currentw + 1;
mpjpt = mp + 1;
for( j=1; j<lgth2+1; j++ )
{
wm = *prept;
*ijppt = 0;
#if 0
fprintf( stderr, "%5.0f->", wm );
#endif
#if 0
fprintf( stderr, "%5.0f?", g );
#endif
if( (g=mi+fpenalty) > wm )
{
wm = g;
*ijppt = -( j - mpi );
}
if( (g=*prept) >= mi )
{
mi = g;
mpi = j-1;
}
#if USE_PENALTY_EX
mi += fpenalty_ex;
#endif
#if 0
fprintf( stderr, "%5.0f?", g );
#endif
if( (g=*mjpt + fpenalty) > wm )
{
wm = g;
*ijppt = +( i - *mpjpt );
}
if( (g=*prept) >= *mjpt )
{
*mjpt = g;
*mpjpt = i-1;
}
#if USE_PENALTY_EX
m[j] += fpenalty_ex;
#endif
#if 0
fprintf( stderr, "%5.0f ", wm );
#endif
*curpt++ += wm;
ijppt++;
mjpt++;
prept++;
mpjpt++;
}
lastverticalw[i] = currentw[lgth2-1]; // lgth2==0 no toki error
}
Atracking( currentw, lastverticalw, seq1, seq2, mseq1, mseq2, ijp );
resultlen = strlen( mseq1[0] );
if( alloclen < resultlen || resultlen > N )
{
fprintf( stderr, "alloclen=%d, resultlen=%d, N=%d\n", alloclen, resultlen, N );
ErrorExit( "LENGTH OVER!\n" );
}
strcpy( seq1[0], mseq1[0] );
strcpy( seq2[0], mseq2[0] );
#if 0
fprintf( stderr, "\n" );
fprintf( stderr, ">\n%s\n", mseq1[0] );
fprintf( stderr, ">\n%s\n", mseq2[0] );
fprintf( stderr, "wm = %f\n", wm );
#endif
return( wm );
}
float MSalign11( char **seq1, char **seq2, int alloclen )
/* score no keisan no sai motokaraaru gap no atukai ni mondai ga aru */
{
// int k;
register int i, j;
int lasti, lastj;
int iin = 0, jin = 0; // by Mathog, a guess
int lgth1, lgth2;
int resultlen;
float wm = 0.0; /* int ?????? */
float g;
float *currentw, *previousw;
float fpenalty = (float)penalty;
#if USE_PENALTY_EX
float fpenalty_ex = (float)penalty_ex;
#endif
float *maxinw = NULL, *maxinwpt = NULL; // by D.Mathog, guess
float *maxinh = NULL; // by D.Mathog, guess
#if 1
float wmmax;
float *wtmp;
int *ijppt;
float *mjpt, *prept, *curpt;
int *mpjpt;
#endif
static float mi, *m;
static int **ijp;
static int mpi, *mp;
static float *w1, *w2;
static float *match;
static float *initverticalw; /* kufuu sureba iranai */
static float *lastverticalw; /* kufuu sureba iranai */
static char **mseq1;
static char **mseq2;
static char **mseq;
static float **cpmx1;
static float **cpmx2;
static int **intwork;
static float **WMMTX;
static float **floatwork;
static int orlgth1 = 0, orlgth2 = 0;
if( orlgth1 == 0 )
{
mseq1 = AllocateCharMtx( njob, 0 );
mseq2 = AllocateCharMtx( njob, 0 );
}
lgth1 = strlen( seq1[0] );
lgth2 = strlen( seq2[0] );
if( lgth1 > orlgth1 || lgth2 > orlgth2 )
{
int ll1, ll2;
if( orlgth1 > 0 && orlgth2 > 0 )
{
FreeFloatVec( w1 );
FreeFloatVec( w2 );
FreeFloatVec( match );
FreeFloatVec( initverticalw );
FreeFloatVec( lastverticalw );
FreeFloatVec( maxinw );
FreeFloatVec( maxinh );
FreeFloatVec( m );
FreeIntVec( mp );
FreeCharMtx( mseq );
FreeFloatMtx( cpmx1 );
FreeFloatMtx( cpmx2 );
FreeFloatMtx( floatwork );
FreeIntMtx( intwork );
}
ll1 = MAX( (int)(1.3*lgth1), orlgth1 ) + 100;
ll2 = MAX( (int)(1.3*lgth2), orlgth2 ) + 100;
#if DEBUG
fprintf( stderr, "\ntrying to allocate (%d+%d)xn matrices ... ", ll1, ll2 );
#endif
w1 = AllocateFloatVec( ll2+2 );
w2 = AllocateFloatVec( ll2+2 );
match = AllocateFloatVec( ll2+2 );
initverticalw = AllocateFloatVec( ll1+2 );
lastverticalw = AllocateFloatVec( ll1+2 );
maxinw = AllocateFloatVec( ll1+2 );
m = AllocateFloatVec( ll2+2 );
mp = AllocateIntVec( ll2+2 );
maxinh = AllocateFloatVec( ll2+2 );
mseq = AllocateCharMtx( njob, ll1+ll2 );
cpmx1 = AllocateFloatMtx( 26, ll1+2 );
cpmx2 = AllocateFloatMtx( 26, ll2+2 );
floatwork = AllocateFloatMtx( 26, MAX( ll1, ll2 )+2 );
intwork = AllocateIntMtx( 26, MAX( ll1, ll2 )+2 );
#if DEBUG
fprintf( stderr, "succeeded\n" );
#endif
orlgth1 = ll1 - 100;
orlgth2 = ll2 - 100;
}
mseq1[0] = mseq[0];
mseq2[0] = mseq[1];
if( orlgth1 > commonAlloc1 || orlgth2 > commonAlloc2 )
{
int ll1, ll2;
if( commonAlloc1 && commonAlloc2 )
{
FreeIntMtx( commonIP );
FreeFloatMtx( WMMTX );
}
ll1 = MAX( orlgth1, commonAlloc1 );
ll2 = MAX( orlgth2, commonAlloc2 );
#if DEBUG
fprintf( stderr, "\n\ntrying to allocate %dx%d matrices ... ", ll1+1, ll2+1 );
#endif
commonIP = AllocateIntMtx( ll1+10, ll2+10 );
WMMTX = AllocateFloatMtx( ll1+10, ll2+10 );
#if DEBUG
fprintf( stderr, "succeeded\n\n" );
#endif
commonAlloc1 = ll1;
commonAlloc2 = ll2;
}
ijp = commonIP;
#if 0
for( i=0; i<lgth1; i++ )
fprintf( stderr, "ogcp1[%d]=%f\n", i, ogcp1[i] );
#endif
currentw = w1;
previousw = w2;
match_calc( initverticalw, seq2, seq1, 0, lgth1 );
match_calc( currentw, seq1, seq2, 0, lgth2 );
WMMTX[0][0] = initverticalw[0];
maxinh[0] = initverticalw[0];
for( i=1; i<lgth1+1; i++ )
{
initverticalw[i] += fpenalty;
WMMTX[i][0] = initverticalw[i];
if( maxinh[0] < initverticalw[i] ) maxinh[0] = initverticalw[i];
}
maxinw[0] = currentw[0];
for( j=1; j<lgth2+1; j++ )
{
currentw[j] += fpenalty;
WMMTX[0][j] = currentw[j];
if( maxinw[0] < currentw[j] ) maxinw[0] = currentw[j];
}
for( j=1; j<lgth2+1; ++j )
{
m[j] = currentw[j-1]; mp[j] = 0;
}
lastverticalw[0] = currentw[lgth2-1];
lasti = lgth1+1;
for( i=1; i<lasti; i++ )
{
wtmp = previousw;
previousw = currentw;
currentw = wtmp;
previousw[0] = initverticalw[i-1];
match_calc( currentw, seq1, seq2, i, lgth2 );
currentw[0] = initverticalw[i];
mi = previousw[0]; mpi = 0;
maxinwpt = maxinw + i;
*maxinwpt = currentw[0];
fprintf( stderr, "currentw[0] = %f, *maxinwpt = %f\n", currentw[0], maxinw[i] );
ijppt = ijp[i] + 1;
mjpt = m + 1;
prept = previousw;
curpt = currentw + 1;
mpjpt = mp + 1;
lastj = lgth2+1;
for( j=1; j<lastj; j++ )
{
wm = *prept;
*ijppt = 0;
#if 0
fprintf( stderr, "%5.0f->", wm );
#endif
g = mi + fpenalty;
#if 0
fprintf( stderr, "%5.0f?", g );
#endif
if( g > wm )
{
wm = g;
*ijppt = -( j - mpi );
}
g = *prept;
if( g >= mi )
{
mi = g;
mpi = j-1;
}
#if USE_PENALTY_EX
mi += fpenalty_ex;
#endif
g = *mjpt + fpenalty;
#if 0
fprintf( stderr, "%5.0f?", g );
#endif
if( g > wm )
{
wm = g;
*ijppt = +( i - *mpjpt );
}
g = *prept;
if( g >= *mjpt )
{
*mjpt = g;
*mpjpt = i-1;
}
#if USE_PENALTY_EX
m[j] += fpenalty_ex;
#endif
#if 0
fprintf( stderr, "%5.0f ", wm );
#endif
*curpt += wm;
WMMTX[i][j] = *curpt;
if( j<lgth2 && *maxinwpt < *curpt ) *maxinwpt = *curpt;
if( j<lgth2 && maxinh[j] < *curpt ) maxinh[j] = *curpt;
// fprintf( stderr, "maxintwpt = %f\n", *maxinwpt );
ijppt++;
mjpt++;
prept++;
mpjpt++;
curpt++;
}
lastverticalw[i] = currentw[lgth2-1];
}
wmmax = -999.9;
for( i=0; i<lgth1; i++ )
{
g = lastverticalw[i];
if( g > wmmax )
{
wmmax = g;
iin = i;
jin = lgth2-1;
}
}
for( j=0; j<lgth2; j++ )
{
g = currentw[j];
if( g > wmmax )
{
wmmax = g;
iin = lgth1-1;
jin = j;
}
}
for( i=0; i<lgth1; i++ )
fprintf( stderr, "maxinw[%d] = %f\n", i, maxinw[i] );
for( j=0; j<lgth2; j++ )
fprintf( stderr, "maxinh[%d] = %f\n", j, maxinh[j] );
fprintf( stderr, "wmmax = %f (%d,%d)\n", wmmax, iin, jin );
if( iin == lgth1 - 1 && jin == lgth2 - 1 )
;
else
wmmax += fpenalty;
fprintf( stderr, "wmmax = %f\n", wmmax );
#if 0
for( i=0; i<lgth1; i++ )
{
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "% 10.2f ", WMMTX[i][j] );
}
fprintf( stderr, "\n" );
}
#endif
mseq1[0] += lgth1+lgth2;
*mseq1[0] = 0;
mseq2[0] += lgth1+lgth2;
*mseq2[0] = 0;
backdp( WMMTX, wmmax, maxinw, maxinh, lgth1, lgth2, alloclen, w1, w2, initverticalw, m, mp, iin, jin, seq1, seq2, mseq1, mseq2 );
fprintf( stderr, "\n" );
#if 1
fprintf( stderr, "\n" );
fprintf( stderr, ">MSres\n%s\n", mseq1[0] );
fprintf( stderr, ">MSres\n%s\n", mseq2[0] );
#endif
#if 0
for( i=0; i<lgth1; i++ )
{
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "% 10.2f ", WMMTX[i][j] );
}
fprintf( stderr, "\n" );
}
#endif
mseq1[0] = mseq[0];
mseq2[0] = mseq[1];
mseq1[0] += lgth1+lgth2;
*mseq1[0] = 0;
mseq2[0] += lgth1+lgth2;
*mseq2[0] = 0;
Atracking( currentw, lastverticalw, seq1, seq2, mseq1, mseq2, cpmx1, cpmx2, ijp );
resultlen = strlen( mseq1[0] );
if( alloclen < resultlen || resultlen > N )
{
fprintf( stderr, "alloclen=%d, resultlen=%d, N=%d\n", alloclen, resultlen, N );
ErrorExit( "LENGTH OVER!\n" );
}
strcpy( seq1[0], mseq1[0] );
strcpy( seq2[0], mseq2[0] );
#if 1
fprintf( stderr, "\n" );
fprintf( stderr, ">\n%s\n", mseq1[0] );
fprintf( stderr, ">\n%s\n", mseq2[0] );
#endif
return( wm );
}
float partA__align( char **seq1, char **seq2, double *eff1, double *eff2, int icyc, int jcyc, int alloclen, LocalHom ***localhom, float *impmatch, int start1, int end1, int start2, int end2, int *gapmap1, int *gapmap2, char *sgap1, char *sgap2, char *egap1, char *egap2 )
/* score no keisan no sai motokaraaru gap no atukai ni mondai ga aru */
{
// int k;
register int i, j;
int lasti, lastj; /* outgap == 0 -> lgth1, outgap == 1 -> lgth1+1 */
int lgth1, lgth2;
int resultlen;
float wm = 0.0; /* int ?????? */
float g;
float *currentw, *previousw;
#if 1
float *wtmp;
int *ijppt;
float *mjpt, *prept, *curpt;
int *mpjpt;
#endif
static float mi, *m;
static int **ijp;
static int mpi, *mp;
static float *w1, *w2;
static float *match;
static float *initverticalw; /* kufuu sureba iranai */
static float *lastverticalw; /* kufuu sureba iranai */
static char **mseq1;
static char **mseq2;
static char **mseq;
static float *ogcp1;
static float *ogcp2;
static float *fgcp1;
static float *fgcp2;
static float **cpmx1;
static float **cpmx2;
static int **intwork;
static float **floatwork;
static int orlgth1 = 0, orlgth2 = 0;
float fpenalty = (float)penalty;
#if USE_PENALTY_EX
float fpenalty_ex = (float)penalty_ex;
#endif
float *fgcp2pt;
float *ogcp2pt;
float fgcp1va;
float ogcp1va;
#if 0
fprintf( stderr, "eff in SA+++align\n" );
for( i=0; i<icyc; i++ ) fprintf( stderr, "eff1[%d] = %f\n", i, eff1[i] );
#endif
if( orlgth1 == 0 )
{
mseq1 = AllocateCharMtx( njob, 0 );
mseq2 = AllocateCharMtx( njob, 0 );
}
lgth1 = strlen( seq1[0] );
lgth2 = strlen( seq2[0] );
if( lgth1 > orlgth1 || lgth2 > orlgth2 )
{
int ll1, ll2;
if( orlgth1 > 0 && orlgth2 > 0 )
{
FreeFloatVec( w1 );
FreeFloatVec( w2 );
FreeFloatVec( match );
FreeFloatVec( initverticalw );
FreeFloatVec( lastverticalw );
FreeFloatVec( m );
FreeIntVec( mp );
FreeCharMtx( mseq );
FreeFloatVec( ogcp1 );
FreeFloatVec( ogcp2 );
FreeFloatVec( fgcp1 );
FreeFloatVec( fgcp2 );
FreeFloatMtx( cpmx1 );
FreeFloatMtx( cpmx2 );
FreeFloatMtx( floatwork );
FreeIntMtx( intwork );
}
ll1 = MAX( (int)(1.3*lgth1), orlgth1 ) + 100;
ll2 = MAX( (int)(1.3*lgth2), orlgth2 ) + 100;
#if DEBUG
fprintf( stderr, "\ntrying to allocate (%d+%d)xn matrices ... ", ll1, ll2 );
#endif
w1 = AllocateFloatVec( ll2+2 );
w2 = AllocateFloatVec( ll2+2 );
match = AllocateFloatVec( ll2+2 );
initverticalw = AllocateFloatVec( ll1+2 );
lastverticalw = AllocateFloatVec( ll1+2 );
m = AllocateFloatVec( ll2+2 );
mp = AllocateIntVec( ll2+2 );
mseq = AllocateCharMtx( njob, ll1+ll2 );
ogcp1 = AllocateFloatVec( ll1+2 );
ogcp2 = AllocateFloatVec( ll2+2 );
fgcp1 = AllocateFloatVec( ll1+2 );
fgcp2 = AllocateFloatVec( ll2+2 );
cpmx1 = AllocateFloatMtx( 26, ll1+2 );
cpmx2 = AllocateFloatMtx( 26, ll2+2 );
#if FASTMATCHCALC
floatwork = AllocateFloatMtx( MAX( ll1, ll2 )+2, 26 );
intwork = AllocateIntMtx( MAX( ll1, ll2 )+2, 26 );
#else
floatwork = AllocateFloatMtx( 26, MAX( ll1, ll2 )+2 );
intwork = AllocateIntMtx( 26, MAX( ll1, ll2 )+2 );
#endif
#if DEBUG
fprintf( stderr, "succeeded\n" );
#endif
orlgth1 = ll1 - 100;
orlgth2 = ll2 - 100;
}
for( i=0; i<icyc; i++ ) mseq1[i] = mseq[i];
for( j=0; j<jcyc; j++ ) mseq2[j] = mseq[icyc+j];
if( orlgth1 > commonAlloc1 || orlgth2 > commonAlloc2 )
{
int ll1, ll2;
if( commonAlloc1 && commonAlloc2 )
{
FreeIntMtx( commonIP );
}
ll1 = MAX( orlgth1, commonAlloc1 );
ll2 = MAX( orlgth2, commonAlloc2 );
#if DEBUG
fprintf( stderr, "\n\ntrying to allocate %dx%d matrices ... ", ll1+1, ll2+1 );
#endif
commonIP = AllocateIntMtx( ll1+10, ll2+10 );
#if DEBUG
fprintf( stderr, "succeeded\n\n" );
#endif
commonAlloc1 = ll1;
commonAlloc2 = ll2;
}
ijp = commonIP;
cpmx_calc_new( seq1, cpmx1, eff1, lgth1, icyc );
cpmx_calc_new( seq2, cpmx2, eff2, lgth2, jcyc );
if( sgap1 )
{
new_OpeningGapCount( ogcp1, icyc, seq1, eff1, lgth1, sgap1 );
new_OpeningGapCount( ogcp2, jcyc, seq2, eff2, lgth2, sgap2 );
new_FinalGapCount( fgcp1, icyc, seq1, eff1, lgth1, egap1 );
new_FinalGapCount( fgcp2, jcyc, seq2, eff2, lgth2, egap2 );
}
else
{
st_OpeningGapCount( ogcp1, icyc, seq1, eff1, lgth1 );
st_OpeningGapCount( ogcp2, jcyc, seq2, eff2, lgth2 );
st_FinalGapCount( fgcp1, icyc, seq1, eff1, lgth1 );
st_FinalGapCount( fgcp2, jcyc, seq2, eff2, lgth2 );
}
for( i=0; i<lgth1; i++ )
{
ogcp1[i] = 0.5 * ( 1.0 - ogcp1[i] ) * fpenalty;
fgcp1[i] = 0.5 * ( 1.0 - fgcp1[i] ) * fpenalty;
}
for( i=0; i<lgth2; i++ )
{
ogcp2[i] = 0.5 * ( 1.0 - ogcp2[i] ) * fpenalty;
fgcp2[i] = 0.5 * ( 1.0 - fgcp2[i] ) * fpenalty;
}
#if 0
for( i=0; i<lgth1; i++ )
fprintf( stderr, "ogcp1[%d]=%f\n", i, ogcp1[i] );
#endif
currentw = w1;
previousw = w2;
match_calc( initverticalw, cpmx2, cpmx1, 0, lgth1, floatwork, intwork, 1 );
if( localhom )
part_imp_match_out_vead_tate_gapmap( initverticalw, gapmap2[0]+start2, lgth1, start1, gapmap1 );
match_calc( currentw, cpmx1, cpmx2, 0, lgth2, floatwork, intwork, 1 );
if( localhom )
part_imp_match_out_vead_gapmap( currentw, gapmap1[0]+start1, lgth2, start2, gapmap2 );
#if 0 // -> tbfast.c
if( localhom )
imp_match_calc( currentw, icyc, jcyc, lgth1, lgth2, seq1, seq2, eff1, eff2, localhom, 1, 0 );
#endif
if( outgap == 1 )
{
for( i=1; i<lgth1+1; i++ )
{
initverticalw[i] += ( ogcp1[0] + fgcp1[i-1] ) ;
}
for( j=1; j<lgth2+1; j++ )
{
currentw[j] += ( ogcp2[0] + fgcp2[j-1] ) ;
}
}
#if OUTGAP0TRY
else
{
for( j=1; j<lgth2+1; j++ )
currentw[j] -= offset * j / 2.0;
for( i=1; i<lgth1+1; i++ )
initverticalw[i] -= offset * i / 2.0;
}
#endif
for( j=1; j<lgth2+1; ++j )
{
m[j] = currentw[j-1] + ogcp1[1]; mp[j] = 0;
}
lastverticalw[0] = currentw[lgth2-1];
if( outgap ) lasti = lgth1+1; else lasti = lgth1;
lastj = lgth2+1;
#if XXXXXXX
fprintf( stderr, "currentw = \n" );
for( i=0; i<lgth1+1; i++ )
{
fprintf( stderr, "%5.2f ", currentw[i] );
}
fprintf( stderr, "\n" );
fprintf( stderr, "initverticalw = \n" );
for( i=0; i<lgth2+1; i++ )
{
fprintf( stderr, "%5.2f ", initverticalw[i] );
}
fprintf( stderr, "\n" );
fprintf( stderr, "fcgp\n" );
for( i=0; i<lgth1; i++ )
fprintf( stderr, "fgcp1[%d]=%f\n", i, ogcp1[i] );
for( i=0; i<lgth2; i++ )
fprintf( stderr, "fgcp2[%d]=%f\n", i, ogcp2[i] );
#endif
for( i=1; i<lasti; i++ )
{
wtmp = previousw;
previousw = currentw;
currentw = wtmp;
previousw[0] = initverticalw[i-1];
match_calc( currentw, cpmx1, cpmx2, i, lgth2, floatwork, intwork, 0 );
#if XXXXXXX
fprintf( stderr, "\n" );
fprintf( stderr, "i=%d\n", i );
fprintf( stderr, "currentw = \n" );
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "%5.2f ", currentw[j] );
}
fprintf( stderr, "\n" );
#endif
if( localhom )
{
// fprintf( stderr, "Calling imp_match_calc (o) lgth = %d, i = %d\n", lgth1, i );
// imp_match_out_vead( currentw, i, lgth2 );
part_imp_match_out_vead_gapmap( currentw, gapmap1[i]+start1, lgth2, start2, gapmap2 );
}
#if XXXXXXX
fprintf( stderr, "\n" );
fprintf( stderr, "i=%d\n", i );
fprintf( stderr, "currentw = \n" );
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "%5.2f ", currentw[j] );
}
fprintf( stderr, "\n" );
#endif
currentw[0] = initverticalw[i];
mi = previousw[0] + ogcp2[1]; mpi = 0;
ijppt = ijp[i] + 1;
mjpt = m + 1;
prept = previousw;
curpt = currentw + 1;
mpjpt = mp + 1;
fgcp2pt = fgcp2;
ogcp2pt = ogcp2+1;
fgcp1va = fgcp1[i-1];
ogcp1va = ogcp1[i];
for( j=1; j<lastj; j++ )
{
wm = *prept;
*ijppt = 0;
#if 0
fprintf( stderr, "%5.0f->", wm );
#endif
g = mi + *fgcp2pt;
#if 0
fprintf( stderr, "%5.0f?", g );
#endif
if( g > wm )
{
wm = g;
*ijppt = -( j - mpi );
}
g = *prept + *ogcp2pt;
if( g >= mi )
{
mi = g;
mpi = j-1;
}
#if USE_PENALTY_EX
mi += fpenalty_ex;
#endif
g = *mjpt + fgcp1va;
#if 0
fprintf( stderr, "%5.0f?", g );
#endif
if( g > wm )
{
wm = g;
*ijppt = +( i - *mpjpt );
}
g = *prept + ogcp1va;
if( g >= *mjpt )
{
*mjpt = g;
*mpjpt = i-1;
}
#if USE_PENALTY_EX
m[j] += fpenalty_ex;
#endif
#if 0
fprintf( stderr, "%5.0f ", wm );
#endif
*curpt += wm;
ijppt++;
mjpt++;
prept++;
mpjpt++;
curpt++;
fgcp2pt++;
ogcp2pt++;
}
lastverticalw[i] = currentw[lgth2-1];
}
#if OUTGAP0TRY
if( !outgap )
{
for( j=1; j<lgth2+1; j++ )
currentw[j] -= offset * ( lgth2 - j ) / 2.0;
for( i=1; i<lgth1+1; i++ )
lastverticalw[i] -= offset * ( lgth1 - i / 2.0);
}
#endif
/*
fprintf( stderr, "\n" );
for( i=0; i<icyc; i++ ) fprintf( stderr,"%s\n", seq1[i] );
fprintf( stderr, "#####\n" );
for( j=0; j<jcyc; j++ ) fprintf( stderr,"%s\n", seq2[j] );
fprintf( stderr, "====>" );
for( i=0; i<icyc; i++ ) strcpy( mseq1[i], seq1[i] );
for( j=0; j<jcyc; j++ ) strcpy( mseq2[j], seq2[j] );
*/
if( localhom )
{
Atracking_localhom( impmatch, currentw, lastverticalw, seq1, seq2, mseq1, mseq2, cpmx1, cpmx2, ijp, icyc, jcyc, start1, end1, start2, end2, gapmap1, gapmap2 );
}
else
Atracking( currentw, lastverticalw, seq1, seq2, mseq1, mseq2, cpmx1, cpmx2, ijp, icyc, jcyc );
// fprintf( stderr, "### impmatch = %f\n", *impmatch );
resultlen = strlen( mseq1[0] );
if( alloclen < resultlen || resultlen > N )
{
fprintf( stderr, "alloclen=%d, resultlen=%d, N=%d\n", alloclen, resultlen, N );
ErrorExit( "LENGTH OVER!\n" );
}
for( i=0; i<icyc; i++ ) strcpy( seq1[i], mseq1[i] );
for( j=0; j<jcyc; j++ ) strcpy( seq2[j], mseq2[j] );
/*
fprintf( stderr, "\n" );
for( i=0; i<icyc; i++ ) fprintf( stderr, "%s\n", mseq1[i] );
fprintf( stderr, "#####\n" );
for( j=0; j<jcyc; j++ ) fprintf( stderr, "%s\n", mseq2[j] );
*/
return( wm );
}
float L__align11( char **seq1, char **seq2, int alloclen, int *off1pt, int *off2pt )
/* score no keisan no sai motokaraaru gap no atukai ni mondai ga aru */
{
// int k;
register int i, j;
int lasti, lastj; /* outgap == 0 -> lgth1, outgap == 1 -> lgth1+1 */
int lgth1, lgth2;
int resultlen;
float wm = 0.0; /* int ?????? */
float g;
float *currentw, *previousw;
#if 1
float *wtmp;
int *ijppt;
float *mjpt, *prept, *curpt;
int *mpjpt;
#endif
static TLS float mi, *m;
static TLS int **ijp;
static TLS int mpi, *mp;
static TLS float *w1, *w2;
static TLS float *match;
static TLS float *initverticalw; /* kufuu sureba iranai */
static TLS float *lastverticalw; /* kufuu sureba iranai */
static TLS char **mseq1;
static TLS char **mseq2;
static TLS char **mseq;
// static TLS int **intwork;
// static TLS float **floatwork;
static TLS int orlgth1 = 0, orlgth2 = 0;
float maxwm;
int endali = 0, endalj = 0; // by D.Mathog, a guess
// int endali, endalj;
float localthr = -offset;
float localthr2 = -offset;
// float localthr = 100;
// float localthr2 = 100;
float fpenalty = (float)penalty;
float fpenalty_ex = (float)penalty_ex;
if( seq1 == NULL )
{
if( orlgth1 > 0 && orlgth2 > 0 )
{
orlgth1 = 0;
orlgth2 = 0;
free( mseq1 );
free( mseq2 );
FreeFloatVec( w1 );
FreeFloatVec( w2 );
FreeFloatVec( match );
FreeFloatVec( initverticalw );
FreeFloatVec( lastverticalw );
FreeFloatVec( m );
FreeIntVec( mp );
FreeCharMtx( mseq );
}
return( 0.0 );
}
if( orlgth1 == 0 )
{
mseq1 = AllocateCharMtx( njob, 0 );
mseq2 = AllocateCharMtx( njob, 0 );
}
lgth1 = strlen( seq1[0] );
lgth2 = strlen( seq2[0] );
if( lgth1 > orlgth1 || lgth2 > orlgth2 )
{
int ll1, ll2;
if( orlgth1 > 0 && orlgth2 > 0 )
{
FreeFloatVec( w1 );
FreeFloatVec( w2 );
FreeFloatVec( match );
FreeFloatVec( initverticalw );
FreeFloatVec( lastverticalw );
FreeFloatVec( m );
FreeIntVec( mp );
FreeCharMtx( mseq );
// FreeFloatMtx( floatwork );
// FreeIntMtx( intwork );
}
ll1 = MAX( (int)(1.3*lgth1), orlgth1 ) + 100;
ll2 = MAX( (int)(1.3*lgth2), orlgth2 ) + 100;
#if DEBUG
fprintf( stderr, "\ntrying to allocate (%d+%d)xn matrices ... ", ll1, ll2 );
#endif
w1 = AllocateFloatVec( ll2+2 );
w2 = AllocateFloatVec( ll2+2 );
match = AllocateFloatVec( ll2+2 );
initverticalw = AllocateFloatVec( ll1+2 );
lastverticalw = AllocateFloatVec( ll1+2 );
m = AllocateFloatVec( ll2+2 );
mp = AllocateIntVec( ll2+2 );
mseq = AllocateCharMtx( njob, ll1+ll2 );
// floatwork = AllocateFloatMtx( 26, MAX( ll1, ll2 )+2 );
// intwork = AllocateIntMtx( 26, MAX( ll1, ll2 )+2 );
#if DEBUG
fprintf( stderr, "succeeded\n" );
#endif
orlgth1 = ll1 - 100;
orlgth2 = ll2 - 100;
}
mseq1[0] = mseq[0];
mseq2[0] = mseq[1];
if( orlgth1 > commonAlloc1 || orlgth2 > commonAlloc2 )
{
int ll1, ll2;
if( commonAlloc1 && commonAlloc2 )
{
FreeIntMtx( commonIP );
}
ll1 = MAX( orlgth1, commonAlloc1 );
ll2 = MAX( orlgth2, commonAlloc2 );
#if DEBUG
fprintf( stderr, "\n\ntrying to allocate %dx%d matrices ... ", ll1+1, ll2+1 );
#endif
commonIP = AllocateIntMtx( ll1+10, ll2+10 );
#if DEBUG
fprintf( stderr, "succeeded\n\n" );
#endif
commonAlloc1 = ll1;
commonAlloc2 = ll2;
}
ijp = commonIP;
#if 0
for( i=0; i<lgth1; i++ )
fprintf( stderr, "ogcp1[%d]=%f\n", i, ogcp1[i] );
#endif
currentw = w1;
previousw = w2;
match_calc( initverticalw, seq2, seq1, 0, lgth1 );
match_calc( currentw, seq1, seq2, 0, lgth2 );
lasti = lgth2+1;
for( j=1; j<lasti; ++j )
{
m[j] = currentw[j-1]; mp[j] = 0;
#if 0
if( m[j] < localthr ) m[j] = localthr2;
#endif
}
lastverticalw[0] = currentw[lgth2-1];
lasti = lgth1+1;
#if 0
fprintf( stderr, "currentw = \n" );
for( i=0; i<lgth1+1; i++ )
{
fprintf( stderr, "%5.2f ", currentw[i] );
}
fprintf( stderr, "\n" );
fprintf( stderr, "initverticalw = \n" );
for( i=0; i<lgth2+1; i++ )
{
fprintf( stderr, "%5.2f ", initverticalw[i] );
}
fprintf( stderr, "\n" );
#endif
#if DEBUG2
fprintf( stderr, "\n" );
fprintf( stderr, " " );
for( j=0; j<lgth2; j++ )
fprintf( stderr, "%c ", seq2[0][j] );
fprintf( stderr, "\n" );
#endif
localstop = lgth1+lgth2+1;
maxwm = -999999999.9;
#if DEBUG2
fprintf( stderr, "\n" );
fprintf( stderr, "%c ", seq1[0][0] );
for( j=0; j<lgth2+1; j++ )
fprintf( stderr, "%5.0f ", currentw[j] );
fprintf( stderr, "\n" );
#endif
for( i=1; i<lasti; i++ )
{
wtmp = previousw;
previousw = currentw;
currentw = wtmp;
previousw[0] = initverticalw[i-1];
match_calc( currentw, seq1, seq2, i, lgth2 );
#if DEBUG2
fprintf( stderr, "%c ", seq1[0][i] );
fprintf( stderr, "%5.0f ", currentw[0] );
#endif
#if XXXXXXX
fprintf( stderr, "\n" );
fprintf( stderr, "i=%d\n", i );
fprintf( stderr, "currentw = \n" );
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "%5.2f ", currentw[j] );
}
fprintf( stderr, "\n" );
#endif
#if XXXXXXX
fprintf( stderr, "\n" );
fprintf( stderr, "i=%d\n", i );
fprintf( stderr, "currentw = \n" );
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "%5.2f ", currentw[j] );
}
fprintf( stderr, "\n" );
#endif
currentw[0] = initverticalw[i];
mi = previousw[0]; mpi = 0;
#if 0
if( mi < localthr ) mi = localthr2;
#endif
ijppt = ijp[i] + 1;
mjpt = m + 1;
prept = previousw;
curpt = currentw + 1;
mpjpt = mp + 1;
lastj = lgth2+1;
for( j=1; j<lastj; j++ )
{
wm = *prept;
*ijppt = 0;
#if 0
fprintf( stderr, "%5.0f->", wm );
#endif
#if 0
fprintf( stderr, "%5.0f?", g );
#endif
if( (g=mi+fpenalty) > wm )
{
wm = g;
*ijppt = -( j - mpi );
}
if( *prept > mi )
{
mi = *prept;
mpi = j-1;
}
#if USE_PENALTY_EX
mi += fpenalty_ex;
#endif
#if 0
fprintf( stderr, "%5.0f?", g );
#endif
if( (g=*mjpt+fpenalty) > wm )
{
wm = g;
*ijppt = +( i - *mpjpt );
}
if( *prept > *mjpt )
{
*mjpt = *prept;
*mpjpt = i-1;
}
#if USE_PENALTY_EX
*mjpt += fpenalty_ex;
#endif
if( maxwm < wm )
{
maxwm = wm;
endali = i;
endalj = j;
}
#if 1
if( wm < localthr )
{
// fprintf( stderr, "stop i=%d, j=%d, curpt=%f\n", i, j, *curpt );
*ijppt = localstop;
wm = localthr2;
}
#endif
#if 0
fprintf( stderr, "%5.0f ", *curpt );
#endif
#if DEBUG2
fprintf( stderr, "%5.0f ", wm );
// fprintf( stderr, "%c-%c *ijppt = %d, localstop = %d\n", seq1[0][i], seq2[0][j], *ijppt, localstop );
#endif
*curpt++ += wm;
ijppt++;
mjpt++;
prept++;
mpjpt++;
}
#if DEBUG2
fprintf( stderr, "\n" );
#endif
lastverticalw[i] = currentw[lgth2-1];
}
#if 0
fprintf( stderr, "maxwm = %f\n", maxwm );
fprintf( stderr, "endali = %d\n", endali );
fprintf( stderr, "endalj = %d\n", endalj );
#endif
if( ijp[endali][endalj] == localstop )
{
strcpy( seq1[0], "" );
strcpy( seq2[0], "" );
*off1pt = *off2pt = 0;
fprintf( stderr, "maxwm <- 0.0 \n" );
return( 0.0 );
}
Ltracking( currentw, lastverticalw, seq1, seq2, mseq1, mseq2, ijp, off1pt, off2pt, endali, endalj );
resultlen = strlen( mseq1[0] );
if( alloclen < resultlen || resultlen > N )
{
fprintf( stderr, "alloclen=%d, resultlen=%d, N=%d\n", alloclen, resultlen, N );
ErrorExit( "LENGTH OVER!\n" );
}
strcpy( seq1[0], mseq1[0] );
strcpy( seq2[0], mseq2[0] );
#if 0
fprintf( stderr, "wm=%f\n", wm );
fprintf( stderr, ">\n%s\n", mseq1[0] );
fprintf( stderr, ">\n%s\n", mseq2[0] );
fprintf( stderr, "maxwm = %f\n", maxwm );
fprintf( stderr, " wm = %f\n", wm );
#endif
return( maxwm );
}
float L__align11( double **n_dynamicmtx, float scoreoffset, char **seq1, char **seq2, int alloclen, int *off1pt, int *off2pt )
/* score no keisan no sai motokaraaru gap no atukai ni mondai ga aru */
{
// int k;
int i, j;
int lasti, lastj; /* outgap == 0 -> lgth1, outgap == 1 -> lgth1+1 */
int lgth1, lgth2;
int resultlen;
float wm = 0.0; /* int ?????? */
float g;
float *currentw, *previousw;
#if 1
float *wtmp;
int *ijppt;
float *mjpt, *prept, *curpt;
int *mpjpt;
#endif
static TLS float mi, *m;
static TLS int **ijp;
static TLS int mpi, *mp;
static TLS float *w1, *w2;
static TLS float *match;
static TLS float *initverticalw; /* kufuu sureba iranai */
static TLS float *lastverticalw; /* kufuu sureba iranai */
static TLS char **mseq1;
static TLS char **mseq2;
static TLS char **mseq;
// static TLS int **intwork;
// static TLS float **floatwork;
static TLS int orlgth1 = 0, orlgth2 = 0;
static TLS double **amino_dynamicmtx = NULL; // ??
float maxwm;
int endali = 0, endalj = 0; // by D.Mathog, a guess
// int endali, endalj;
float localthr = -offset + scoreoffset * 600; // 2013/12/13
float localthr2 = -offset + scoreoffset * 600; // 2013/12/13
// float localthr = -offset;
// float localthr2 = -offset;
float fpenalty = (float)penalty;
float fpenalty_ex = (float)penalty_ex;
float fpenalty_shift = (float)penalty_shift;
float fpenalty_tmp; // atode kesu
int *warpis = NULL;
int *warpjs = NULL;
int *warpi = NULL;
int *warpj = NULL;
int *prevwarpi = NULL;
int *prevwarpj = NULL;
float *wmrecords = NULL;
float *prevwmrecords = NULL;
int warpn = 0;
int warpbase;
float curm = 0.0;
float *wmrecordspt, *wmrecords1pt, *prevwmrecordspt;
int *warpipt, *warpjpt;
if( seq1 == NULL )
{
if( orlgth1 > 0 && orlgth2 > 0 )
{
orlgth1 = 0;
orlgth2 = 0;
free( mseq1 );
free( mseq2 );
FreeFloatVec( w1 );
FreeFloatVec( w2 );
FreeFloatVec( match );
FreeFloatVec( initverticalw );
FreeFloatVec( lastverticalw );
FreeFloatVec( m );
FreeIntVec( mp );
FreeCharMtx( mseq );
if( amino_dynamicmtx ) FreeDoubleMtx( amino_dynamicmtx ); amino_dynamicmtx = NULL;
}
return( 0.0 );
}
if( orlgth1 == 0 )
{
mseq1 = AllocateCharMtx( njob, 0 );
mseq2 = AllocateCharMtx( njob, 0 );
}
lgth1 = strlen( seq1[0] );
lgth2 = strlen( seq2[0] );
warpbase = lgth1 + lgth2;
warpis = NULL;
warpjs = NULL;
warpn = 0;
if( trywarp )
{
wmrecords = AllocateFloatVec( lgth2+1 );
warpi = AllocateIntVec( lgth2+1 );
warpj = AllocateIntVec( lgth2+1 );
prevwmrecords = AllocateFloatVec( lgth2+1 );
prevwarpi = AllocateIntVec( lgth2+1 );
prevwarpj = AllocateIntVec( lgth2+1 );
for( i=0; i<lgth2+1; i++ ) prevwmrecords[i] = 0.0;
for( i=0; i<lgth2+1; i++ ) wmrecords[i] = 0.0;
for( i=0; i<lgth2+1; i++ ) prevwarpi[i] = -warpbase;
for( i=0; i<lgth2+1; i++ ) prevwarpj[i] = -warpbase;
for( i=0; i<lgth2+1; i++ ) warpi[i] = -warpbase;
for( i=0; i<lgth2+1; i++ ) warpj[i] = -warpbase;
}
if( lgth1 > orlgth1 || lgth2 > orlgth2 )
{
int ll1, ll2;
if( orlgth1 > 0 && orlgth2 > 0 )
{
FreeFloatVec( w1 );
FreeFloatVec( w2 );
FreeFloatVec( match );
FreeFloatVec( initverticalw );
FreeFloatVec( lastverticalw );
FreeFloatVec( m );
FreeIntVec( mp );
FreeCharMtx( mseq );
if( amino_dynamicmtx ) FreeDoubleMtx( amino_dynamicmtx ); amino_dynamicmtx = NULL;
// FreeFloatMtx( floatwork );
// FreeIntMtx( intwork );
}
ll1 = MAX( (int)(1.3*lgth1), orlgth1 ) + 100;
ll2 = MAX( (int)(1.3*lgth2), orlgth2 ) + 100;
#if DEBUG
fprintf( stderr, "\ntrying to allocate (%d+%d)xn matrices ... ", ll1, ll2 );
#endif
w1 = AllocateFloatVec( ll2+2 );
w2 = AllocateFloatVec( ll2+2 );
match = AllocateFloatVec( ll2+2 );
initverticalw = AllocateFloatVec( ll1+2 );
lastverticalw = AllocateFloatVec( ll1+2 );
m = AllocateFloatVec( ll2+2 );
mp = AllocateIntVec( ll2+2 );
mseq = AllocateCharMtx( njob, ll1+ll2 );
// floatwork = AllocateFloatMtx( nalphabets, MAX( ll1, ll2 )+2 );
// intwork = AllocateIntMtx( nalphabets, MAX( ll1, ll2 )+2 );
#if DEBUG
fprintf( stderr, "succeeded\n" );
#endif
amino_dynamicmtx = AllocateDoubleMtx( 0x80, 0x80 );
orlgth1 = ll1 - 100;
orlgth2 = ll2 - 100;
}
for( i=0; i<nalphabets; i++) for( j=0; j<nalphabets; j++ )
amino_dynamicmtx[(int)amino[i]][(int)amino[j]] = (double)n_dynamicmtx[i][j];
mseq1[0] = mseq[0];
mseq2[0] = mseq[1];
if( orlgth1 > commonAlloc1 || orlgth2 > commonAlloc2 )
{
int ll1, ll2;
if( commonAlloc1 && commonAlloc2 )
{
FreeIntMtx( commonIP );
}
ll1 = MAX( orlgth1, commonAlloc1 );
ll2 = MAX( orlgth2, commonAlloc2 );
#if DEBUG
fprintf( stderr, "\n\ntrying to allocate %dx%d matrices ... ", ll1+1, ll2+1 );
#endif
commonIP = AllocateIntMtx( ll1+10, ll2+10 );
#if DEBUG
fprintf( stderr, "succeeded\n\n" );
#endif
commonAlloc1 = ll1;
commonAlloc2 = ll2;
}
ijp = commonIP;
#if 0
for( i=0; i<lgth1; i++ )
fprintf( stderr, "ogcp1[%d]=%f\n", i, ogcp1[i] );
#endif
currentw = w1;
previousw = w2;
match_calc_mtx( amino_dynamicmtx, initverticalw, seq2, seq1, 0, lgth1 );
match_calc_mtx( amino_dynamicmtx, currentw, seq1, seq2, 0, lgth2 );
lasti = lgth2+1;
for( j=1; j<lasti; ++j )
{
m[j] = currentw[j-1]; mp[j] = 0;
#if 0
if( m[j] < localthr ) m[j] = localthr2;
#endif
}
lastverticalw[0] = currentw[lgth2-1];
lasti = lgth1+1;
#if 0
fprintf( stderr, "currentw = \n" );
for( i=0; i<lgth1+1; i++ )
{
fprintf( stderr, "%5.2f ", currentw[i] );
}
fprintf( stderr, "\n" );
fprintf( stderr, "initverticalw = \n" );
for( i=0; i<lgth2+1; i++ )
{
fprintf( stderr, "%5.2f ", initverticalw[i] );
}
fprintf( stderr, "\n" );
#endif
#if DEBUG2
fprintf( stderr, "\n" );
fprintf( stderr, " " );
for( j=0; j<lgth2; j++ )
fprintf( stderr, "%c ", seq2[0][j] );
fprintf( stderr, "\n" );
#endif
localstop = lgth1+lgth2+1;
maxwm = -999999999.9;
#if DEBUG2
fprintf( stderr, "\n" );
fprintf( stderr, "%c ", seq1[0][0] );
for( j=0; j<lgth2+1; j++ )
fprintf( stderr, "%5.0f ", currentw[j] );
fprintf( stderr, "\n" );
#endif
for( i=1; i<lasti; i++ )
{
wtmp = previousw;
previousw = currentw;
currentw = wtmp;
previousw[0] = initverticalw[i-1];
match_calc_mtx( amino_dynamicmtx, currentw, seq1, seq2, i, lgth2 );
#if DEBUG2
fprintf( stderr, "%c ", seq1[0][i] );
fprintf( stderr, "%5.0f ", currentw[0] );
#endif
#if XXXXXXX
fprintf( stderr, "\n" );
fprintf( stderr, "i=%d\n", i );
fprintf( stderr, "currentw = \n" );
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "%5.2f ", currentw[j] );
}
fprintf( stderr, "\n" );
#endif
#if XXXXXXX
fprintf( stderr, "\n" );
fprintf( stderr, "i=%d\n", i );
fprintf( stderr, "currentw = \n" );
for( j=0; j<lgth2; j++ )
{
fprintf( stderr, "%5.2f ", currentw[j] );
}
fprintf( stderr, "\n" );
#endif
currentw[0] = initverticalw[i];
mi = previousw[0]; mpi = 0;
#if 0
if( mi < localthr ) mi = localthr2;
#endif
ijppt = ijp[i] + 1;
mjpt = m + 1;
prept = previousw;
curpt = currentw + 1;
mpjpt = mp + 1;
lastj = lgth2+1;
if( trywarp )
{
prevwmrecordspt = prevwmrecords;
wmrecordspt = wmrecords+1;
wmrecords1pt = wmrecords;
warpipt = warpi + 1;
warpjpt = warpj + 1;
}
for( j=1; j<lastj; j++ )
{
wm = *prept;
*ijppt = 0;
#if 0
fprintf( stderr, "%5.0f->", wm );
#endif
#if 0
fprintf( stderr, "%5.0f?", g );
#endif
if( (g=mi+fpenalty) > wm )
{
wm = g;
*ijppt = -( j - mpi );
}
if( *prept > mi )
{
mi = *prept;
mpi = j-1;
}
#if USE_PENALTY_EX
mi += fpenalty_ex;
#endif
#if 0
fprintf( stderr, "%5.0f?", g );
#endif
if( (g=*mjpt+fpenalty) > wm )
{
wm = g;
*ijppt = +( i - *mpjpt );
}
if( *prept > *mjpt )
{
*mjpt = *prept;
*mpjpt = i-1;
}
#if USE_PENALTY_EX
*mjpt += fpenalty_ex;
#endif
if( maxwm < wm )
{
maxwm = wm;
endali = i;
endalj = j;
}
#if 1
if( wm < localthr )
{
// fprintf( stderr, "stop i=%d, j=%d, curpt=%f, localthr = %f\n", i, j, *curpt, localthr );
*ijppt = localstop;
wm = localthr2;
}
#endif
#if 0
fprintf( stderr, "%5.0f ", *curpt );
#endif
#if 0
fprintf( stderr, "wm (%d,%d) = %5.0f\n", i, j, wm );
// fprintf( stderr, "%c-%c *ijppt = %d, localstop = %d\n", seq1[0][i], seq2[0][j], *ijppt, localstop );
#endif
if( trywarp )
{
fpenalty_tmp = fpenalty_shift + fpenalty_ex * ( i - prevwarpi[j-1] + j - prevwarpj[j-1] );
// fprintf( stderr, "fpenalty_shift = %f\n", fpenalty_tmp );
// fprintf( stderr, "\n\n\nwarp to %c-%c (%d-%d) from %c-%c (%d-%d) ? prevwmrecords[%d] = %f + %f <- wm = %f\n", seq1[0][prevwarpi[j-1]], seq2[0][prevwarpj[j-1]], prevwarpi[j-1], prevwarpj[j-1], seq1[0][i], seq2[0][j], i, j, j, prevwmrecords[j-1], fpenalty_tmp, wm );
// if( (g=prevwmrecords[j-1] + fpenalty_shift )> wm )
if( ( g=*prevwmrecordspt++ + fpenalty_tmp )> wm ) // naka ha osokute kamawanai
{
// fprintf( stderr, "Yes! Warp!! from %d-%d (%c-%c) to %d-%d (%c-%c) fpenalty_tmp = %f! warpn = %d\n", i, j, seq1[0][i], seq2[0][j-1], prevwarpi[j-1], prevwarpj[j-1],seq1[0][prevwarpi[j-1]], seq2[0][prevwarpj[j-1]], fpenalty_tmp, warpn );
if( warpn && prevwarpi[j-1] == warpis[warpn-1] && prevwarpj[j-1] == warpjs[warpn-1] )
{
*ijppt = warpbase + warpn - 1;
}
else
{
*ijppt = warpbase + warpn;
warpis = realloc( warpis, sizeof(int) * ( warpn+1 ) );
warpjs = realloc( warpjs, sizeof(int) * ( warpn+1 ) );
warpis[warpn] = prevwarpi[j-1];
warpjs[warpn] = prevwarpj[j-1];
warpn++;
}
wm = g;
}
else
{
}
curm = *curpt + wm;
// fprintf( stderr, "###### curm = %f at %c-%c, i=%d, j=%d\n", curm, seq1[0][i], seq2[0][j], i, j );
// fprintf( stderr, "copy from i, j-1? %f > %f?\n", wmrecords[j-1], curm );
// if( wmrecords[j-1] > wmrecords[j] )
if( *wmrecords1pt > *wmrecordspt )
{
// fprintf( stderr, "yes\n" );
// wmrecords[j] = wmrecords[j-1];
*wmrecordspt = *wmrecords1pt;
// warpi[j] = warpi[j-1];
// warpj[j] = warpj[j-1];
*warpipt = *(warpipt-1);
*warpjpt = *(warpjpt-1);
// fprintf( stderr, "warpi[j]=%d, warpj[j]=%d wmrecords[j] = %f\n", warpi[j], warpj[j], wmrecords[j] );
}
// else
// {
// fprintf( stderr, "no\n" );
// }
// fprintf( stderr, " curm = %f at %c-%c\n", curm, seq1[0][i], seq2[0][j] );
// fprintf( stderr, " wmrecords[%d] = %f\n", j, wmrecords[j] );
// fprintf( stderr, "replace?\n" );
// if( curm > wmrecords[j] )
if( curm > *wmrecordspt )
{
// fprintf( stderr, "yes at %d-%d (%c-%c), replaced warp: warpi[j]=%d, warpj[j]=%d warpn=%d, wmrecords[j] = %f -> %f\n", i, j, seq1[0][i], seq2[0][j], i, j, warpn, wmrecords[j], curm );
// wmrecords[j] = curm;
*wmrecordspt = curm;
// warpi[j] = i;
// warpj[j] = j;
*warpipt = i;
*warpjpt = j;
}
// else
// {
// fprintf( stderr, "No! warpi[j]=%d, warpj[j]=%d wmrecords[j] = %f\n", warpi[j], warpj[j], wmrecords[j] );
// }
// fprintf( stderr, "%d-%d (%c-%c) curm = %5.0f, wmrecords[j]=%f\n", i, j, seq1[0][i], seq2[0][j], curm, wmrecords[j] );
wmrecordspt++;
wmrecords1pt++;
warpipt++;
warpjpt++;
}
*curpt++ += wm;
ijppt++;
mjpt++;
prept++;
mpjpt++;
}
#if DEBUG2
fprintf( stderr, "\n" );
#endif
lastverticalw[i] = currentw[lgth2-1];
if( trywarp )
{
fltncpy( prevwmrecords, wmrecords, lastj );
intncpy( prevwarpi, warpi, lastj );
intncpy( prevwarpj, warpj, lastj );
}
}
// fprintf( stderr, "\nwm = %f\n", wm );
if( trywarp )
{
// if( warpn ) fprintf( stderr, "warpn = %d\n", warpn );
free( wmrecords );
free( prevwmrecords );
free( warpi );
free( warpj );
free( prevwarpi );
free( prevwarpj );
}
#if 0
fprintf( stderr, "maxwm = %f\n", maxwm );
fprintf( stderr, "endali = %d\n", endali );
fprintf( stderr, "endalj = %d\n", endalj );
#endif
if( ijp[endali][endalj] == localstop )
{
strcpy( seq1[0], "" );
strcpy( seq2[0], "" );
*off1pt = *off2pt = 0;
fprintf( stderr, "maxwm <- 0.0 \n" );
return( 0.0 );
}
Ltracking( currentw, lastverticalw, seq1, seq2, mseq1, mseq2, ijp, off1pt, off2pt, endali, endalj, warpis, warpjs, warpbase );
if( warpis ) free( warpis );
if( warpjs ) free( warpjs );
resultlen = strlen( mseq1[0] );
if( alloclen < resultlen || resultlen > N )
{
fprintf( stderr, "alloclen=%d, resultlen=%d, N=%d\n", alloclen, resultlen, N );
ErrorExit( "LENGTH OVER!\n" );
}
strcpy( seq1[0], mseq1[0] );
strcpy( seq2[0], mseq2[0] );
#if 0
fprintf( stderr, "wm=%f\n", wm );
fprintf( stderr, ">\n%s\n", mseq1[0] );
fprintf( stderr, ">\n%s\n", mseq2[0] );
fprintf( stderr, "maxwm = %f\n", maxwm );
fprintf( stderr, " wm = %f\n", wm );
#endif
return( maxwm );
}
float Falign_localhom( char **seq1, char **seq2,
double *eff1, double *eff2,
int clus1, int clus2,
int alloclen,
LocalHom ***localhom, float *totalimpmatch,
int *gapmap1, int *gapmap2,
int *chudanpt, int chudanref, int *chudanres )
{
// tditeration.c deha alloclen ha huhen nanode
// prevalloclen ha iranai.
int i, j, k, l, m, maxk;
int nlen, nlen2, nlen4;
static TLS int crossscoresize = 0;
static TLS char **tmpseq1 = NULL;
static TLS char **tmpseq2 = NULL;
static TLS char **tmpptr1 = NULL;
static TLS char **tmpptr2 = NULL;
static TLS char **tmpres1 = NULL;
static TLS char **tmpres2 = NULL;
static TLS char **result1 = NULL;
static TLS char **result2 = NULL;
#if RND
static TLS char **rndseq1 = NULL;
static TLS char **rndseq2 = NULL;
#endif
static TLS Fukusosuu **seqVector1 = NULL;
static TLS Fukusosuu **seqVector2 = NULL;
static TLS Fukusosuu **naiseki = NULL;
static TLS Fukusosuu *naisekiNoWa = NULL;
static TLS double *soukan = NULL;
static TLS double **crossscore = NULL;
int nlentmp;
static TLS int *kouho = NULL;
static TLS Segment *segment = NULL;
static TLS Segment *segment1 = NULL;
static TLS Segment *segment2 = NULL;
static TLS Segment **sortedseg1 = NULL;
static TLS Segment **sortedseg2 = NULL;
static TLS int *cut1 = NULL;
static TLS int *cut2 = NULL;
static TLS char *sgap1, *egap1, *sgap2, *egap2;
static TLS int localalloclen = 0;
int lag;
int tmpint;
int count, count0;
int len1, len2;
int totallen;
float totalscore;
float impmatch;
extern Fukusosuu *AllocateFukusosuuVec();
extern Fukusosuu **AllocateFukusosuuMtx();
if( seq1 == NULL )
{
if( result1 )
{
// fprintf( stderr, "Freeing localarrays in Falign\n" );
localalloclen = 0;
mymergesort( 0, 0, NULL );
alignableReagion( 0, 0, NULL, NULL, NULL, NULL, NULL );
fft( 0, NULL, 1 );
A__align( NULL, NULL, NULL, NULL, 0, 0, 0, NULL, NULL, NULL, NULL, NULL, NULL, NULL, 0, NULL, 0, 0 );
G__align11( NULL, NULL, 0, 0, 0 );
partA__align( NULL, NULL, NULL, NULL, 0, 0, 0, NULL, NULL, 0, 0, 0, 0, NULL, NULL, NULL, NULL, NULL, NULL, NULL, 0, NULL );
blockAlign2( NULL, NULL, NULL, NULL, NULL, NULL );
if( crossscore ) FreeDoubleMtx( crossscore );
FreeCharMtx( result1 );
FreeCharMtx( result2 );
FreeCharMtx( tmpres1 );
FreeCharMtx( tmpres2 );
FreeCharMtx( tmpseq1 );
FreeCharMtx( tmpseq2 );
free( sgap1 );
free( egap1 );
free( sgap2 );
free( egap2 );
free( kouho );
free( cut1 );
free( cut2 );
free( tmpptr1 );
free( tmpptr2 );
free( segment );
free( segment1 );
free( segment2 );
free( sortedseg1 );
free( sortedseg2 );
if( !kobetsubunkatsu )
{
FreeFukusosuuMtx ( seqVector1 );
FreeFukusosuuMtx ( seqVector2 );
FreeFukusosuuVec( naisekiNoWa );
FreeFukusosuuMtx( naiseki );
FreeDoubleVec( soukan );
}
}
else
{
// fprintf( stderr, "Did not allocate localarrays in Falign\n" );
}
return( 0.0 );
}
len1 = strlen( seq1[0] );
len2 = strlen( seq2[0] );
nlentmp = MAX( len1, len2 );
nlen = 1;
while( nlentmp >= nlen ) nlen <<= 1;
#if 0
fprintf( stderr, "### nlen = %d\n", nlen );
#endif
nlen2 = nlen/2; nlen4 = nlen2 / 2;
#if DEBUG
fprintf( stderr, "len1 = %d, len2 = %d\n", len1, len2 );
fprintf( stderr, "nlentmp = %d, nlen = %d\n", nlentmp, nlen );
#endif
if( !localalloclen )
{
sgap1 = AllocateCharVec( njob );
egap1 = AllocateCharVec( njob );
sgap2 = AllocateCharVec( njob );
egap2 = AllocateCharVec( njob );
kouho = AllocateIntVec( NKOUHO );
cut1 = AllocateIntVec( MAXSEG );
cut2 = AllocateIntVec( MAXSEG );
tmpptr1 = AllocateCharMtx( njob, 0 );
tmpptr2 = AllocateCharMtx( njob, 0 );
result1 = AllocateCharMtx( njob, alloclen );
result2 = AllocateCharMtx( njob, alloclen );
tmpres1 = AllocateCharMtx( njob, alloclen );
tmpres2 = AllocateCharMtx( njob, alloclen );
// crossscore = AllocateDoubleMtx( MAXSEG, MAXSEG );
segment = (Segment *)calloc( MAXSEG, sizeof( Segment ) );
segment1 = (Segment *)calloc( MAXSEG, sizeof( Segment ) );
segment2 = (Segment *)calloc( MAXSEG, sizeof( Segment ) );
sortedseg1 = (Segment **)calloc( MAXSEG, sizeof( Segment * ) );
sortedseg2 = (Segment **)calloc( MAXSEG, sizeof( Segment * ) );
if( !( segment && segment1 && segment2 && sortedseg1 && sortedseg2 ) )
ErrorExit( "Allocation error\n" );
if ( scoremtx == -1 ) n20or4or2 = 4;
else if( fftscore == 1 ) n20or4or2 = 2;
else n20or4or2 = 20;
}
if( localalloclen < nlen )
{
if( localalloclen )
{
#if 1
if( !kobetsubunkatsu )
{
FreeFukusosuuMtx ( seqVector1 );
FreeFukusosuuMtx ( seqVector2 );
FreeFukusosuuVec( naisekiNoWa );
FreeFukusosuuMtx( naiseki );
FreeDoubleVec( soukan );
}
FreeCharMtx( tmpseq1 );
FreeCharMtx( tmpseq2 );
#endif
#if RND
FreeCharMtx( rndseq1 );
FreeCharMtx( rndseq2 );
#endif
}
tmpseq1 = AllocateCharMtx( njob, nlen );
tmpseq2 = AllocateCharMtx( njob, nlen );
if( !kobetsubunkatsu )
{
naisekiNoWa = AllocateFukusosuuVec( nlen );
naiseki = AllocateFukusosuuMtx( n20or4or2, nlen );
seqVector1 = AllocateFukusosuuMtx( n20or4or2+1, nlen+1 );
seqVector2 = AllocateFukusosuuMtx( n20or4or2+1, nlen+1 );
soukan = AllocateDoubleVec( nlen+1 );
}
#if RND
rndseq1 = AllocateCharMtx( njob, nlen );
rndseq2 = AllocateCharMtx( njob, nlen );
for( i=0; i<njob; i++ )
{
generateRndSeq( rndseq1[i], nlen );
generateRndSeq( rndseq2[i], nlen );
}
#endif
localalloclen = nlen;
}
for( j=0; j<clus1; j++ ) strcpy( tmpseq1[j], seq1[j] );
for( j=0; j<clus2; j++ ) strcpy( tmpseq2[j], seq2[j] );
#if 0
fftfp = fopen( "input_of_Falign", "w" );
fprintf( fftfp, "nlen = %d\n", nlen );
fprintf( fftfp, "seq1: ( %d sequences ) \n", clus1 );
for( i=0; i<clus1; i++ )
fprintf( fftfp, "%s\n", seq1[i] );
fprintf( fftfp, "seq2: ( %d sequences ) \n", clus2 );
for( i=0; i<clus2; i++ )
fprintf( fftfp, "%s\n", seq2[i] );
fclose( fftfp );
system( "less input_of_Falign < /dev/tty > /dev/tty" );
#endif
if( !kobetsubunkatsu )
{
fprintf( stderr, "FFT ... " );
for( j=0; j<n20or4or2; j++ ) vec_init( seqVector1[j], nlen );
if( fftscore && scoremtx != -1 )
{
for( i=0; i<clus1; i++ )
{
seq_vec_2( seqVector1[0], polarity, eff1[i], tmpseq1[i] );
seq_vec_2( seqVector1[1], volume, eff1[i], tmpseq1[i] );
}
}
else
{
#if 0
for( i=0; i<clus1; i++ ) for( j=0; j<n20or4or2; j++ )
seq_vec( seqVector1[j], amino[j], eff1[i], tmpseq1[i] );
#else
for( i=0; i<clus1; i++ )
seq_vec_3( seqVector1, eff1[i], tmpseq1[i] );
#endif
}
#if RND
for( i=0; i<clus1; i++ )
{
vec_init2( seqVector1, rndseq1[i], eff1[i], len1, nlen );
}
#endif
#if 0
fftfp = fopen( "seqVec", "w" );
fprintf( fftfp, "before transform\n" );
for( k=0; k<n20or4or2; k++ )
{
fprintf( fftfp, "nlen=%d\n", nlen );
fprintf( fftfp, "%c\n", amino[k] );
for( l=0; l<nlen; l++ )
fprintf( fftfp, "%f %f\n", seqVector1[k][l].R, seqVector1[k][l].I );
}
fclose( fftfp );
system( "less seqVec < /dev/tty > /dev/tty" );
#endif
for( j=0; j<n20or4or2; j++ ) vec_init( seqVector2[j], nlen );
if( fftscore && scoremtx != -1 )
{
for( i=0; i<clus2; i++ )
{
seq_vec_2( seqVector2[0], polarity, eff2[i], tmpseq2[i] );
seq_vec_2( seqVector2[1], volume, eff2[i], tmpseq2[i] );
}
}
else
{
#if 0
for( i=0; i<clus2; i++ ) for( j=0; j<n20or4or2; j++ )
seq_vec( seqVector2[j], amino[j], eff2[i], tmpseq2[i] );
#else
for( i=0; i<clus2; i++ )
seq_vec_3( seqVector2, eff2[i], tmpseq2[i] );
#endif
}
#if RND
for( i=0; i<clus2; i++ )
{
vec_init2( seqVector2, rndseq2[i], eff2[i], len2, nlen );
}
#endif
#if 0
fftfp = fopen( "seqVec2", "w" );
fprintf( fftfp, "before fft\n" );
for( k=0; k<n20or4or2; k++ )
{
fprintf( fftfp, "%c\n", amino[k] );
for( l=0; l<nlen; l++ )
fprintf( fftfp, "%f %f\n", seqVector2[k][l].R, seqVector2[k][l].I );
}
fclose( fftfp );
system( "less seqVec2 < /dev/tty > /dev/tty" );
#endif
for( j=0; j<n20or4or2; j++ )
{
fft( nlen, seqVector2[j], (j==0) );
fft( nlen, seqVector1[j], 0 );
}
#if 0
fftfp = fopen( "seqVec2", "w" );
fprintf( fftfp, "#after fft\n" );
for( k=0; k<n20or4or2; k++ )
{
fprintf( fftfp, "#%c\n", amino[k] );
for( l=0; l<nlen; l++ )
fprintf( fftfp, "%f %f\n", seqVector2[k][l].R, seqVector2[k][l].I );
}
fclose( fftfp );
system( "less seqVec2 < /dev/tty > /dev/tty" );
#endif
for( k=0; k<n20or4or2; k++ )
{
for( l=0; l<nlen; l++ )
calcNaiseki( naiseki[k]+l, seqVector1[k]+l, seqVector2[k]+l );
}
for( l=0; l<nlen; l++ )
{
naisekiNoWa[l].R = 0.0;
naisekiNoWa[l].I = 0.0;
for( k=0; k<n20or4or2; k++ )
{
naisekiNoWa[l].R += naiseki[k][l].R;
naisekiNoWa[l].I += naiseki[k][l].I;
}
}
#if 0
fftfp = fopen( "naisekiNoWa", "w" );
fprintf( fftfp, "#Before fft\n" );
for( l=0; l<nlen; l++ )
fprintf( fftfp, "%d %f %f\n", l, naisekiNoWa[l].R, naisekiNoWa[l].I );
fclose( fftfp );
system( "less naisekiNoWa < /dev/tty > /dev/tty " );
#endif
fft( -nlen, naisekiNoWa, 0 );
for( m=0; m<=nlen2; m++ )
soukan[m] = naisekiNoWa[nlen2-m].R;
for( m=nlen2+1; m<nlen; m++ )
soukan[m] = naisekiNoWa[nlen+nlen2-m].R;
#if 0
fftfp = fopen( "naisekiNoWa", "w" );
fprintf( fftfp, "#After fft\n" );
for( l=0; l<nlen; l++ )
fprintf( fftfp, "%d %f\n", l, naisekiNoWa[l].R );
fclose( fftfp );
fftfp = fopen( "list.plot", "w" );
fprintf( fftfp, "plot 'naisekiNoWa'\npause -1" );
fclose( fftfp );
system( "/usr/bin/gnuplot list.plot &" );
#endif
#if 0
fprintf( stderr, "frt write start\n" );
fftfp = fopen( "frt", "w" );
for( l=0; l<nlen; l++ )
fprintf( fftfp, "%d %f\n", l-nlen2, soukan[l] );
fclose( fftfp );
system( "less frt < /dev/tty > /dev/tty" );
#if 0
fftfp = fopen( "list.plot", "w" );
fprintf( fftfp, "plot 'frt'\n pause +1" );
fclose( fftfp );
system( "/usr/bin/gnuplot list.plot" );
#endif
#endif
getKouho( kouho, NKOUHO, soukan, nlen );
#if 0
for( i=0; i<NKOUHO; i++ )
{
fprintf( stderr, "kouho[%d] = %d\n", i, kouho[i] );
}
#endif
}
#if KEIKA
fprintf( stderr, "Searching anchors ... " );
#endif
count = 0;
#define CAND 0
#if CAND
fftfp = fopen( "cand", "w" );
fclose( fftfp );
#endif
if( kobetsubunkatsu )
{
maxk = 1;
kouho[0] = 0;
}
else
{
maxk = NKOUHO;
}
for( k=0; k<maxk; k++ )
{
lag = kouho[k];
zurasu2( lag, clus1, clus2, seq1, seq2, tmpptr1, tmpptr2 );
#if CAND
fftfp = fopen( "cand", "a" );
fprintf( fftfp, "Candidate No.%d lag = %d\n", k+1, lag );
fprintf( fftfp, "%s\n", tmpptr1[0] );
fprintf( fftfp, "%s\n", tmpptr2[0] );
fclose( fftfp );
#endif
tmpint = alignableReagion( clus1, clus2, tmpptr1, tmpptr2, eff1, eff2, segment+count );
if( count+tmpint > MAXSEG -3 ) ErrorExit( "TOO MANY SEGMENTS.\n" );
while( tmpint-- > 0 )
{
if( lag > 0 )
{
segment1[count].start = segment[count].start ;
segment1[count].end = segment[count].end ;
segment1[count].center = segment[count].center;
segment1[count].score = segment[count].score;
segment2[count].start = segment[count].start + lag;
segment2[count].end = segment[count].end + lag;
segment2[count].center = segment[count].center + lag;
segment2[count].score = segment[count].score ;
}
else
{
segment1[count].start = segment[count].start - lag;
segment1[count].end = segment[count].end - lag;
segment1[count].center = segment[count].center - lag;
segment1[count].score = segment[count].score ;
segment2[count].start = segment[count].start ;
segment2[count].end = segment[count].end ;
segment2[count].center = segment[count].center;
segment2[count].score = segment[count].score ;
}
#if 0
fftfp = fopen( "cand", "a" );
fprintf( fftfp, "Goukaku=%dko\n", tmpint );
fprintf( fftfp, "in 1 %d\n", segment1[count].center );
fprintf( fftfp, "in 2 %d\n", segment2[count].center );
fclose( fftfp );
#endif
segment1[count].pair = &segment2[count];
segment2[count].pair = &segment1[count];
count++;
#if 0
fprintf( stderr, "count=%d\n", count );
#endif
}
}
#if 1
if( !kobetsubunkatsu )
fprintf( stderr, "%d segments found\n", count );
#endif
if( !count && fftNoAnchStop )
ErrorExit( "Cannot detect anchor!" );
#if 0
fftfp = fopen( "fft", "a" );
fprintf( fftfp, "RESULT before sort:\n" );
for( l=0; l<count; l++ )
{
fprintf( fftfp, "cut[%d]=%d, ", l, segment1[l].center );
fprintf( fftfp, "%d score = %f\n", segment2[l].center, segment1[l].score );
}
fclose( fftfp );
#endif
#if KEIKA
fprintf( stderr, "Aligning anchors ... " );
#endif
for( i=0; i<count; i++ )
{
sortedseg1[i] = &segment1[i];
sortedseg2[i] = &segment2[i];
}
#if 0
tmpsort( count, sortedseg1 );
tmpsort( count, sortedseg2 );
qsort( sortedseg1, count, sizeof( Segment * ), segcmp );
qsort( sortedseg2, count, sizeof( Segment * ), segcmp );
#else
mymergesort( 0, count-1, sortedseg1 );
mymergesort( 0, count-1, sortedseg2 );
#endif
for( i=0; i<count; i++ ) sortedseg1[i]->number = i;
for( i=0; i<count; i++ ) sortedseg2[i]->number = i;
if( kobetsubunkatsu )
{
for( i=0; i<count; i++ )
{
cut1[i+1] = sortedseg1[i]->center;
cut2[i+1] = sortedseg2[i]->center;
}
cut1[0] = 0;
cut2[0] = 0;
cut1[count+1] = len1;
cut2[count+1] = len2;
count += 2;
}
else
{
if( crossscoresize < count+2 )
{
crossscoresize = count+2;
#if 1
fprintf( stderr, "######allocating crossscore, size = %d\n", crossscoresize );
#endif
if( crossscore ) FreeDoubleMtx( crossscore );
crossscore = AllocateDoubleMtx( crossscoresize, crossscoresize );
}
for( i=0; i<count+2; i++ ) for( j=0; j<count+2; j++ )
crossscore[i][j] = 0.0;
for( i=0; i<count; i++ )
{
crossscore[segment1[i].number+1][segment1[i].pair->number+1] = segment1[i].score;
cut1[i+1] = sortedseg1[i]->center;
cut2[i+1] = sortedseg2[i]->center;
}
#if DEBUG
fprintf( stderr, "AFTER SORT\n" );
for( i=0; i<count; i++ ) fprintf( stderr, "%d, %d\n", segment1[i].start, segment2[i].start );
#endif
crossscore[0][0] = 10000000.0;
cut1[0] = 0;
cut2[0] = 0;
crossscore[count+1][count+1] = 10000000.0;
cut1[count+1] = len1;
cut2[count+1] = len2;
count += 2;
count0 = count;
blockAlign2( cut1, cut2, sortedseg1, sortedseg2, crossscore, &count );
if( count0 > count )
{
#if 0
fprintf( stderr, "\7 REPEAT!? \n" );
#else
fprintf( stderr, "REPEAT!? \n" );
#endif
if( fftRepeatStop ) exit( 1 );
}
#if KEIKA
else fprintf( stderr, "done\n" );
#endif
}
#if 0
fftfp = fopen( "fft", "a" );
fprintf( fftfp, "RESULT after sort:\n" );
for( l=0; l<count; l++ )
{
fprintf( fftfp, "cut[%d]=%d, ", l, segment1[l].center );
fprintf( fftfp, "%d\n", segment2[l].center );
}
fclose( fftfp );
#endif
#if 0
fftfp = fopen( "fft", "a" );
fprintf( fftfp, "RESULT after sort:\n" );
for( l=0; l<count; l++ )
{
fprintf( fftfp, "cut : %d %d\n", cut1[l], cut2[l] );
}
fclose( fftfp );
#endif
#if KEIKA
fprintf( trap_g, "Devided to %d segments\n", count-1 );
fprintf( trap_g, "%d %d forg\n", MIN( clus1, clus2 ), count-1 );
#endif
totallen = 0;
for( j=0; j<clus1; j++ ) result1[j][0] = 0;
for( j=0; j<clus2; j++ ) result2[j][0] = 0;
totalscore = 0.0;
*totalimpmatch = 0.0;
for( i=0; i<count-1; i++ )
{
#if DEBUG
fprintf( stderr, "DP %03d / %03d %4d to ", i+1, count-1, totallen );
#else
#if KEIKA
fprintf( stderr, "DP %03d / %03d\r", i+1, count-1 );
#endif
#endif
if( cut1[i] )
{
getkyokaigap( sgap1, seq1, cut1[i]-1, clus1 );
getkyokaigap( sgap2, seq2, cut2[i]-1, clus2 );
}
else
{
for( j=0; j<clus1; j++ ) sgap1[j] = 'o';
for( j=0; j<clus2; j++ ) sgap2[j] = 'o';
}
if( cut1[i+1] != len1 )
{
getkyokaigap( egap1, seq1, cut1[i+1], clus1 );
getkyokaigap( egap2, seq2, cut2[i+1], clus2 );
}
else
{
for( j=0; j<clus1; j++ ) egap1[j] = 'o';
for( j=0; j<clus2; j++ ) egap2[j] = 'o';
}
for( j=0; j<clus1; j++ )
{
strncpy( tmpres1[j], seq1[j]+cut1[i], cut1[i+1]-cut1[i] );
tmpres1[j][cut1[i+1]-cut1[i]] = 0;
}
if( kobetsubunkatsu ) commongappick_record( clus1, tmpres1, gapmap1 );
for( j=0; j<clus2; j++ )
{
strncpy( tmpres2[j], seq2[j]+cut2[i], cut2[i+1]-cut2[i] );
tmpres2[j][cut2[i+1]-cut2[i]] = 0;
}
if( kobetsubunkatsu ) commongappick_record( clus2, tmpres2, gapmap2 );
#if 0
fprintf( stderr, "count = %d\n", count );
fprintf( stderr, "### reg1 = %d-%d\n", cut1[i], cut1[i+1]-1 );
fprintf( stderr, "### reg2 = %d-%d\n", cut2[i], cut2[i+1]-1 );
#endif
switch( alg )
{
case( 'a' ):
totalscore += Aalign( tmpres1, tmpres2, eff1, eff2, clus1, clus2, alloclen );
break;
case( 'Q' ):
totalscore += partQ__align( tmpres1, tmpres2, eff1, eff2, clus1, clus2, alloclen, localhom, &impmatch, cut1[i], cut1[i+1]-1, cut2[i], cut2[i+1]-1, gapmap1, gapmap2, sgap1, sgap2, egap1, egap2 );
*totalimpmatch += impmatch;
// fprintf( stderr, "*totalimpmatch in Falign_localhom = %f\n", *totalimpmatch );
break;
case( 'A' ):
totalscore += partA__align( tmpres1, tmpres2, eff1, eff2, clus1, clus2, alloclen, localhom, &impmatch, cut1[i], cut1[i+1]-1, cut2[i], cut2[i+1]-1, gapmap1, gapmap2, sgap1, sgap2, egap1, egap2, chudanpt, chudanref, chudanres );
*totalimpmatch += impmatch;
// fprintf( stderr, "*totalimpmatch in Falign_localhom = %f\n", *totalimpmatch );
break;
default:
fprintf( stderr, "alg = %c\n", alg );
ErrorExit( "ERROR IN SOURCE FILE Falign.c" );
break;
}
#ifdef enablemultithread
if( chudanres && *chudanres )
{
// fprintf( stderr, "\n\n## CHUUDAN!!! at Falign_localhom\n" );
return( -1.0 );
}
#endif
nlen = strlen( tmpres1[0] );
if( totallen + nlen > alloclen )
{
fprintf( stderr, "totallen=%d + nlen=%d > alloclen = %d\n", totallen, nlen, alloclen );
ErrorExit( "LENGTH OVER in Falign\n " );
}
for( j=0; j<clus1; j++ ) strcat( result1[j], tmpres1[j] );
for( j=0; j<clus2; j++ ) strcat( result2[j], tmpres2[j] );
totallen += nlen;
#if 0
fprintf( stderr, "%4d\r", totallen );
fprintf( stderr, "\n\n" );
for( j=0; j<clus1; j++ )
{
fprintf( stderr, "%s\n", tmpres1[j] );
}
fprintf( stderr, "-------\n" );
for( j=0; j<clus2; j++ )
{
fprintf( stderr, "%s\n", tmpres2[j] );
}
#endif
}
#if KEIKA
fprintf( stderr, "DP ... done \n" );
#endif
for( j=0; j<clus1; j++ ) strcpy( seq1[j], result1[j] );
for( j=0; j<clus2; j++ ) strcpy( seq2[j], result2[j] );
#if 0
for( j=0; j<clus1; j++ )
{
fprintf( stderr, "%s\n", result1[j] );
}
fprintf( stderr, "- - - - - - - - - - -\n" );
for( j=0; j<clus2; j++ )
{
fprintf( stderr, "%s\n", result2[j] );
}
#endif
return( totalscore );
}
