int main(int argc, char **argv) { char *indx, imem[PATH_MAX]; int fd; off_t off; ssize_t siz, rem, tot; bwaidx_t *idx; if (argc != 2) { fprintf(stderr, "usage: %s <index>\n", basename(*argv)); return 1; } indx = *(argv+1); sprintf(imem, "%s.map", indx); idx = bwa_idx_load(indx, BWA_IDX_ALL); assert(idx != NULL); assert(bwa_idx2mem(idx) == 0); fd = open(imem, O_WRONLY|O_CREAT|O_TRUNC, 0666); assert(fd != -1); off = 0; rem = idx->l_mem; tot = 0; while (rem > 0) { siz = write(fd, idx->mem + off, (size_t)rem); assert(siz != -1); off += siz; rem -= siz; tot += siz; } assert(tot == (ssize_t)idx->l_mem); assert(close(fd) != -1); return 0; }
int main(int argc, char *argv[]) { bwaidx_t *idx; gzFile fp; kseq_t *ks; mem_opt_t *opt; if (argc < 3) { fprintf(stderr, "Usage: bwamem-lite <idx.base> <reads.fq>\n"); return 1; } idx = bwa_idx_load(argv[1], BWA_IDX_ALL); // load the BWA index if (NULL == idx) { fprintf(stderr, "Index load failed.\n"); exit(EXIT_FAILURE); } fp = strcmp(argv[2], "-")? gzopen(argv[2], "r") : gzdopen(fileno(stdin), "r"); if (NULL == fp) { fprintf(stderr, "Couldn't open %s : %s\n", strcmp(argv[2], "-") ? argv[2] : "stdin", errno ? strerror(errno) : "Out of memory"); exit(EXIT_FAILURE); } ks = kseq_init(fp); // initialize the FASTA/Q parser opt = mem_opt_init(); // initialize the BWA-MEM parameters to the default values while (kseq_read(ks) >= 0) { // read one sequence mem_alnreg_v ar; int i, k; ar = mem_align1(opt, idx->bwt, idx->bns, idx->pac, ks->seq.l, ks->seq.s); // get all the hits for (i = 0; i < ar.n; ++i) { // traverse each hit mem_aln_t a; if (ar.a[i].secondary >= 0) continue; // skip secondary alignments a = mem_reg2aln(opt, idx->bns, idx->pac, ks->seq.l, ks->seq.s, &ar.a[i]); // get forward-strand position and CIGAR // print alignment err_printf("%s\t%c\t%s\t%ld\t%d\t", ks->name.s, "+-"[a.is_rev], idx->bns->anns[a.rid].name, (long)a.pos, a.mapq); for (k = 0; k < a.n_cigar; ++k) // print CIGAR err_printf("%d%c", a.cigar[k]>>4, "MIDSH"[a.cigar[k]&0xf]); err_printf("\t%d\n", a.NM); // print edit distance free(a.cigar); // don't forget to deallocate CIGAR } free(ar.a); // and deallocate the hit list } free(opt); kseq_destroy(ks); err_gzclose(fp); bwa_idx_destroy(idx); return 0; }
int bwa_bwtsw2(int argc, char *argv[]) { bsw2opt_t *opt; bwaidx_t *idx; int c; opt = bsw2_init_opt(); srand48(11); while ((c = getopt(argc, argv, "q:r:a:b:t:T:w:d:z:m:s:c:N:Hf:MI:SG:C")) >= 0) { switch (c) { case 'q': opt->q = atoi(optarg); break; case 'r': opt->r = atoi(optarg); break; case 'a': opt->a = atoi(optarg); break; case 'b': opt->b = atoi(optarg); break; case 'w': opt->bw = atoi(optarg); break; case 'T': opt->t = atoi(optarg); break; case 't': opt->n_threads = atoi(optarg); break; case 'z': opt->z = atoi(optarg); break; case 's': opt->is = atoi(optarg); break; case 'm': opt->mask_level = atof(optarg); break; case 'c': opt->coef = atof(optarg); break; case 'N': opt->t_seeds = atoi(optarg); break; case 'M': opt->multi_2nd = 1; break; case 'H': opt->hard_clip = 1; break; case 'f': xreopen(optarg, "w", stdout); break; case 'I': opt->max_ins = atoi(optarg); break; case 'S': opt->skip_sw = 1; break; case 'C': opt->cpy_cmt = 1; break; case 'G': opt->max_chain_gap = atoi(optarg); break; } } opt->qr = opt->q + opt->r; if (optind + 2 > argc) { fprintf(stderr, "\n"); fprintf(stderr, "Usage: bwa bwasw [options] <target.prefix> <query.fa> [query2.fa]\n\n"); fprintf(stderr, "Options: -a INT score for a match [%d]\n", opt->a); fprintf(stderr, " -b INT mismatch penalty [%d]\n", opt->b); fprintf(stderr, " -q INT gap open penalty [%d]\n", opt->q); fprintf(stderr, " -r INT gap extension penalty [%d]\n", opt->r); fprintf(stderr, " -w INT band width [%d]\n", opt->bw); fprintf(stderr, " -m FLOAT mask level [%.2f]\n", opt->mask_level); fprintf(stderr, "\n"); fprintf(stderr, " -t INT number of threads [%d]\n", opt->n_threads); fprintf(stderr, " -f FILE file to output results to instead of stdout\n"); fprintf(stderr, " -H in SAM output, use hard clipping instead of soft clipping\n"); fprintf(stderr, " -C copy FASTA/Q comment to SAM output\n"); fprintf(stderr, " -M mark multi-part alignments as secondary\n"); fprintf(stderr, " -S skip Smith-Waterman read pairing\n"); fprintf(stderr, " -I INT ignore pairs with insert >=INT for inferring the size distr [%d]\n", opt->max_ins); fprintf(stderr, "\n"); fprintf(stderr, " -T INT score threshold divided by a [%d]\n", opt->t); fprintf(stderr, " -c FLOAT coefficient of length-threshold adjustment [%.1f]\n", opt->coef); fprintf(stderr, " -z INT Z-best [%d]\n", opt->z); fprintf(stderr, " -s INT maximum seeding interval size [%d]\n", opt->is); fprintf(stderr, " -N INT # seeds to trigger rev aln; 2*INT is also the chaining threshold [%d]\n", opt->t_seeds); fprintf(stderr, " -G INT maximum gap size during chaining [%d]\n", opt->max_chain_gap); fprintf(stderr, "\n"); fprintf(stderr, "Note: For long Illumina, 454 and Sanger reads, assembly contigs, fosmids and\n"); fprintf(stderr, " BACs, the default setting usually works well. For the current PacBio\n"); fprintf(stderr, " reads (end of 2010), '-b5 -q2 -r1 -z10' is recommended. One may also\n"); fprintf(stderr, " increase '-z' for better sensitivity.\n"); fprintf(stderr, "\n"); return 1; } // adjust opt for opt->a opt->t *= opt->a; opt->coef *= opt->a; if ((idx = bwa_idx_load(argv[optind], BWA_IDX_BWT|BWA_IDX_BNS)) == 0) return 0; bsw2_aln(opt, idx->bns, idx->bwt, argv[optind+1], optind+2 < argc? argv[optind+2] : 0); bwa_idx_destroy(idx); free(opt); return 0; }