static int extract_feature_visitor_feature_node(GtNodeVisitor *nv,
GtFeatureNode *fn, GtError *err)
{
GtExtractFeatureVisitor *efv;
GtFeatureNodeIterator *fni;
GtFeatureNode *child;
GtStrArray *target_ids = NULL;
GtStr *seqid = NULL,
*description,
*sequence;
int had_err = 0;
gt_error_check(err);
efv = gt_extract_feature_visitor_cast(nv);
gt_assert(efv->region_mapping);
fni = gt_feature_node_iterator_new(fn);
if (efv->target)
target_ids = gt_str_array_new();
if (efv->seqid)
seqid = gt_str_new();
description = gt_str_new();
sequence = gt_str_new();
while (!had_err && (child = gt_feature_node_iterator_next(fni))) {
if (seqid)
gt_str_reset(seqid);
if (target_ids)
gt_str_array_reset(target_ids);
if (gt_extract_feature_sequence(sequence, (GtGenomeNode*) child, efv->type,
efv->join, seqid, target_ids,
efv->region_mapping, err)) {
had_err = -1;
}
if (!had_err && gt_str_length(sequence)) {
efv->fastaseq_counter++;
construct_description(description, efv->type, efv->fastaseq_counter,
efv->join, efv->translate, seqid, target_ids);
had_err = show_entry(description, sequence, efv->translate, efv->width,
efv->outfp);
gt_str_reset(description);
gt_str_reset(sequence);
}
}
gt_str_delete(sequence);
gt_str_delete(description);
gt_str_delete(seqid);
gt_str_array_delete(target_ids);
gt_feature_node_iterator_delete(fni);
return had_err;
}
static int update_seq_col_if_necessary(GtRegionMapping *rm, GtStr *seqid,
GtError *err)
{
int had_err = 0;
gt_error_check(err);
gt_assert(rm && seqid);
/* for mappings, we need to load the changed sequence, if needed... */
if (rm->mapping) {
if (!rm->sequence_file || (gt_str_cmp(rm->sequence_name, seqid))) {
gt_str_delete(rm->sequence_file);
/* ignore MD5 hashes when using region mappings */
if (gt_md5_seqid_has_prefix(gt_str_get(seqid))) {
rm->sequence_file = region_mapping_map(rm,
gt_str_get(seqid)
+GT_MD5_SEQID_TOTAL_LEN,
err);
} else
rm->sequence_file = region_mapping_map(rm, gt_str_get(seqid), err);
if (!rm->sequence_file)
had_err = -1;
else {
/* load new seqcol */
if (!rm->sequence_filenames)
rm->sequence_filenames = gt_str_array_new();
else
gt_str_array_reset(rm->sequence_filenames);
gt_str_array_add(rm->sequence_filenames, rm->sequence_file);
if (!rm->sequence_name)
rm->sequence_name = gt_str_new();
else
gt_str_reset(rm->sequence_name);
gt_str_append_str(rm->sequence_name, seqid);
gt_seq_col_delete(rm->seq_col);
rm->seq_col = gt_bioseq_col_new(rm->sequence_filenames, err);
if (!rm->seq_col)
had_err = -1;
}
}
} else {
/* ...otherwise, just make sure the seqcol is loaded */
if (!rm->seq_col) {
if (rm->encseq) {
if (!(rm->seq_col = gt_encseq_col_new(rm->encseq, err)))
had_err = -1;
} else {
gt_assert(rm->sequence_filenames);
if (!(rm->seq_col = gt_bioseq_col_new(rm->sequence_filenames, err)))
had_err = -1;
}
}
if (!had_err && rm->usedesc) {
if (rm->seqid2seqnum_mapping)
gt_seqid2seqnum_mapping_delete(rm->seqid2seqnum_mapping);
rm->seqid2seqnum_mapping =
gt_seqid2seqnum_mapping_new_seqcol(rm->seq_col, err);
if (!rm->seqid2seqnum_mapping) {
had_err = -1;
}
}
}
return had_err;
}
static int gt_extract_feature_sequence_generic(GtStr *sequence,
GtGenomeNode *gn,
const char *type, bool join, GtStr *seqid,
GtStrArray *target_ids,
unsigned int *out_phase_offset,
GtRegionMapping *region_mapping, GtError *err)
{
GtFeatureNode *fn;
GtRange range;
unsigned int phase_offset = 0;
char *outsequence;
const char *target;
int had_err = 0;
gt_error_check(err);
fn = gt_genome_node_cast(gt_feature_node_class(), gn);
gt_assert(fn);
if (seqid)
gt_str_append_str(seqid, gt_genome_node_get_seqid(gn));
if (target_ids &&
(target = gt_feature_node_get_attribute(fn, GT_GFF_TARGET))) {
had_err = gt_gff3_parser_parse_all_target_attributes(target, false,
target_ids, NULL,
NULL, "", 0, err);
}
if (!had_err) {
if (join) {
GtFeatureNodeIterator *fni;
GtFeatureNode *child;
bool reverse_strand = false,
first_child = true,
first_child_of_type_seen = false;
GtPhase phase = GT_PHASE_UNDEFINED;
/* in this case we have to traverse the children */
fni = gt_feature_node_iterator_new_direct(gt_feature_node_cast(gn));
while (!had_err && (child = gt_feature_node_iterator_next(fni))) {
if (first_child) {
if (target_ids &&
(target = gt_feature_node_get_attribute(child, GT_GFF_TARGET))) {
gt_str_array_reset(target_ids);
had_err = gt_gff3_parser_parse_all_target_attributes(target, false,
target_ids,
NULL,
NULL, "", 0,
err);
}
first_child = false;
}
if (!had_err) {
if (extract_join_feature((GtGenomeNode*) child, type, region_mapping,
sequence, &reverse_strand,
&first_child_of_type_seen,
&phase, err)) {
had_err = -1;
}
if (phase != GT_PHASE_UNDEFINED) {
phase_offset = (int) phase;
}
}
}
gt_feature_node_iterator_delete(fni);
gt_assert(phase_offset <= (unsigned int) GT_PHASE_UNDEFINED);
if (!had_err && gt_str_length(sequence)) {
if (reverse_strand) {
had_err = gt_reverse_complement(gt_str_get(sequence),
gt_str_length(sequence), err);
}
}
}
else if (gt_feature_node_get_type(fn) == type) {
GtPhase phase = gt_feature_node_get_phase(fn);
gt_assert(!had_err);
if (phase != GT_PHASE_UNDEFINED)
phase_offset = (unsigned int) phase;
/* otherwise we only have to look at this feature */
range = gt_genome_node_get_range(gn);
gt_assert(range.start); /* 1-based coordinates */
had_err = gt_region_mapping_get_sequence(region_mapping, &outsequence,
gt_genome_node_get_seqid(gn),
range.start, range.end, err);
if (!had_err) {
gt_str_append_cstr_nt(sequence, outsequence, gt_range_length(&range));
gt_free(outsequence);
if (gt_feature_node_get_strand(fn) == GT_STRAND_REVERSE) {
had_err = gt_reverse_complement(gt_str_get(sequence),
gt_str_length(sequence), err);
}
}
}
}
if (out_phase_offset && phase_offset != GT_PHASE_UNDEFINED) {
*out_phase_offset = phase_offset;
}
return had_err;
}
