예제 #1
0
static void restover_read_equiv(AjPFile equfile, AjPTable table)
{
    AjPStr line;
    AjPStr key;
    AjPStr value;

    const char *p;

    line = ajStrNew();

    while(ajReadlineTrim(equfile,&line))
    {
	p=ajStrGetPtr(line);
	if(!*p || *p=='#' || *p=='!')
	    continue;
	p=ajSysFuncStrtok(p," \t\n");
	key=ajStrNewC(p);
	p=ajSysFuncStrtok(NULL," \t\n");
	value=ajStrNewC(p);
	ajTablePut(table,(void *)key, (void *)value);
    }

    ajStrDel(&line);

    return;
}
예제 #2
0
static void matPushHitInt(const AjPStr n, const EmbPMatPrints m,
			  AjPList *l, ajint pos,
			  ajint score, ajint elem, ajint hpe, ajint hpm)
{
    EmbPMatMatch mat;

    AJNEW0 (mat);
    mat->seqname = ajStrNewC(ajStrGetPtr(n));
    mat->cod     = ajStrNewC(ajStrGetPtr((m)->cod));
    mat->acc     = ajStrNewC(ajStrGetPtr((m)->acc));
    mat->tit     = ajStrNewC(ajStrGetPtr((m)->tit));
    mat->pat     = ajStrNew();
    mat->n       = (m)->n;
    mat->len     = (m)->len[elem];
    mat->thresh  = (m)->thresh[elem];
    mat->max     = (m)->max[elem];
    mat->element = elem;
    mat->start   = pos;
    mat->score   = score;
    mat->hpe     = hpe;
    mat->hpm     = hpm;
    mat->all	 = ajFalse;
    mat->ordered = ajFalse;

    ajListPush(*l,(void *)mat);

    return;
}
예제 #3
0
static void remap_read_equiv(AjPFile *equfile, AjPTable *table,
			     AjBool commercial)
{
    AjPStr line;
    AjPStr key;
    AjPStr value;

    const char *p;

    line = ajStrNew();

    while(ajReadlineTrim(*equfile,&line))
    {
        p = ajStrGetPtr(line);

        if(!*p || *p=='#' || *p=='!')
            continue;
        p = ajSysFuncStrtok(p," \t\n");
        key = ajStrNewC(p);
        p = ajSysFuncStrtok(NULL," \t\n");
        value = ajStrNewC(p);
	if(!commercial)
	    ajStrTrimEndC(&value,"*");
        ajTablePut(*table,(void *)key, (void *)value);
    }

    ajFileClose(equfile);
    ajStrDel(&line);

    return;
}
예제 #4
0
파일: gfile.c 프로젝트: ktnyt/GEMBASSY
ajint gValID(AjPStr id){
  AjPFilebuff buff = NULL;
  AjPStr      url  = NULL;
  AjPStr      line = NULL;
  AjPRegexp   pval = NULL;

  url = ajStrNewC("http://web.sfc.keio.ac.jp/~t11080hi/valID/valID.cgi?id=");
  url = ajStrNew();
  ajFmtPrintS(&url, "http://rest.g-language.org/%S", id);

  //ajStrAppendS(&url, id);

  if(!gFilebuffURLS(url, &buff)) {
    return ajFalse;
  }

  return ajTrue;

  ajBuffreadLine(buff, &line);

  pval = ajRegCompC("^0");

  if(ajRegExec(pval, line))
    return ajFalse;

  return ajTrue;
}
예제 #5
0
AjBool embMiscMatchPatternDelimC (const AjPStr str, const AjPStr pattern,
                                 const char* delim)
{

    char whiteSpace[] = " \t\n\r";  /* skip whitespace */
    AjPStrTok tokens;
    AjPStr delimstr = NULL;
    AjPStr key = NULL;
    AjBool val = ajFalse;

    delimstr = ajStrNewC(whiteSpace);
    ajStrAppendC(&delimstr, delim);
    tokens = ajStrTokenNewS(pattern, delimstr);

    while (ajStrTokenNextParse( &tokens, &key))
	if (ajStrMatchWildCaseS(str, key))
	{
	    val = ajTrue;
	    break;
	}

    ajStrTokenDel( &tokens);
    ajStrDel(&key);
    ajStrDel(&delimstr);

    return val;

}
예제 #6
0
AjBool gFormatGenbank(AjPSeq seq, AjPStr *inseq){
  AjPSeqout     seqout   = NULL;
  AjPFeattabOut featout  = NULL;
  AjPFeattable  feat     = NULL;
  AjPStr        seqline  = NULL;
  AjPStr        featline = NULL;
  AjPFile       seqfile  = NULL;
  AjPFile       featfile = NULL;
  AjPStr        filename = NULL;

  gAssignUniqueName(&filename);
  feat = ajSeqGetFeatCopy(seq);

  if(!feat)
    return ajFalse;

  seqout = ajSeqoutNew();

  if(!ajSeqoutOpenFilename(seqout,filename))
    embExitBad();

  ajSeqoutSetFormatS(seqout,ajStrNewC("genbank"));
  ajSeqoutWriteSeq(seqout,seq);
  ajSeqoutClose(seqout);
  ajSeqoutDel(&seqout);

  seqfile = ajFileNewInNameS(filename);
  ajSysFileUnlinkS(filename);

  featout = ajFeattabOutNew();

  if(!ajFeattabOutOpen(featout,filename))
    return ajFalse;

  ajFeattableWriteGenbank(featout,feat);

  ajFeattableDel(&feat);
  //ajFeattabOutDel(&featout);
  ajFileClose(&(featout->Handle));

  featfile = ajFileNewInNameS(filename);
  ajSysFileUnlinkS(filename);

  while(ajReadline(seqfile,&seqline)){
    if(ajStrMatchC(seqline,"ORIGIN\n")){
      while(ajReadline(featfile,&featline)){
	ajStrAppendS(inseq, featline);
      }
    }
    ajStrAppendS(inseq, seqline);
  }

  ajStrDel(&seqline);
  ajStrDel(&featline);
  ajStrDel(&filename);
  ajFileClose(&seqfile);
  ajFileClose(&featfile);

  return ajTrue;
}
예제 #7
0
파일: ghttp.c 프로젝트: ktnyt/GEMBASSY
AjBool gHttpGetBinC(char* url, AjPFile* outf)
{
  if(!gHttpGetBinS(ajStrNewC(url), outf))
    return ajFalse;

  return ajTrue;
}
예제 #8
0
파일: tfscan.c 프로젝트: WenchaoLin/JAMg
static void tfscan_print_hits(AjPList *l,
			      ajint hits, AjPReport outf,
			      const AjPTable t,
			      const AjPSeq seq, ajuint minlength,
			      const  AjPTable btable)
{
    ajint i;
    EmbPMatMatch m;
    const AjPStr acc = NULL;
    const AjPStr bf  = NULL;
    AjPFeattable ftable = NULL;
    AjPFeature gf  = NULL;
    AjPStr type = ajStrNewC("SO:0000235");
    AjPStr tmpstr = NULL;

    ftable = ajFeattableNewSeq(seq);

    /*ajFmtPrintF(outf,"TFSCAN of %s from %d to %d\n\n",ajStrGetPtr(name),
      begin,end);*/

    for(i=0;i<hits;++i)
    {
	ajListPop(*l,(void **)&m);
	acc = ajTableFetchS(t, m->seqname);

	if(m->len >= minlength)
        {
	    /*ajFmtPrintF(outf,"%-20s %-8s %-5d %-5d %s\n",
                        ajStrGetPtr(m->seqname),
			ajStrGetPtr(acc),m->start,
			m->start+m->len-1,ajStrGetPtr(s));*/
            if(m->forward)
                gf = ajFeatNew(ftable, ajUtilGetProgram(), type,
                               m->start,
                               m->start+m->len-1,
                               (float) m->score,
                               '+',0);
            else
                gf = ajFeatNew(ftable, ajUtilGetProgram(), type,
                               m->start,
                               m->start+m->len-1,
                               (float) m->score,
                               '-',0);
            ajFmtPrintS(&tmpstr, "*acc %S", acc);
            ajFeatTagAddSS(gf, NULL, tmpstr);
            bf  = ajTableFetchS(btable, m->seqname);
            ajFmtPrintS(&tmpstr, "*factor %S", bf);
            ajFeatTagAddSS(gf, NULL, tmpstr);
        }

	embMatMatchDel(&m);
    }

    ajReportWrite(outf, ftable, seq);
    ajFeattableDel(&ftable);
    ajStrDel(&tmpstr);
    ajStrDel(&type);

    return;
}
예제 #9
0
static AjPFeattable dastestFeatureQuery(const AjPStr ufo,
                                        ajint ibegin, ajint iend)
{
    AjPFeattabin ftquery;
    AjPFeattable fttable;
    AjPStr fmt;

    fttable =  ajFeattableNew(NULL);

    fmt = ajStrNewC("dasgff");

    ftquery = ajFeattabinNewSS(fmt,ufo,"any");


    ftquery->Start = ibegin;
    ftquery->End = iend;

    ajStrAssignS(&ftquery->Input->Qry, ufo);

    ftquery->Input->Query->DataType = AJDATATYPE_FEATURES;

    ajFilebuffDel(&ftquery->Input->Filebuff);

    ajFeattabinRead(ftquery, fttable);

    ajStrDel(&fmt);

    ajFeattabinClear(ftquery);
    ajFeattabinDel(&ftquery);

    return fttable;
}
예제 #10
0
int main(int argc, char **argv) {
  // initialize EMBASSY info
  embInitPV("kclique", argc, argv, "KBWS", "1.0.9");

  struct soap soap;
  char*  jobid;
  char*  result;

  AjPFile    infile;
  AjPFile    outf;
  AjPStr     substr;
  AjPStr     indata = NULL;
  AjPStr     line   = NULL;

  infile = ajAcdGetInfile("infile");
  outf   = ajAcdGetOutfile("outfile");

  while (ajReadline(infile, &line)) {
    ajStrAppendS(&indata, line);
    ajStrAppendC(&indata, "\n");
  }

  soap_init(&soap);

  char* in0;
  in0 = ajCharNewS(indata);
  if ( soap_call_ns1__runClique( &soap, NULL, NULL, in0, &jobid ) == SOAP_OK ) {
  } else {
    soap_print_fault(&soap, stderr);
  }

  int check = 0;
  while ( check == 0 ) {
    if ( soap_call_ns1__checkStatus( &soap, NULL, NULL, jobid,  &check ) == SOAP_OK ) {
    } else {
      soap_print_fault(&soap, stderr);
    }
    sleep(3);
  }

  if ( soap_call_ns1__getResult( &soap, NULL, NULL, jobid,  &result ) == SOAP_OK ) {
    substr = ajStrNewC(result);
    ajFmtPrintF(outf,"%S\n",substr);
  } else {
    soap_print_fault(&soap, stderr);
  }

  soap_destroy(&soap);
  soap_end(&soap);
  soap_done(&soap);

  ajFileClose(&infile);
  ajFileClose(&outf);
  ajStrDel(&substr);

  embExit();

  return 0;
}
예제 #11
0
파일: gfile.c 프로젝트: ktnyt/GEMBASSY
AjBool gStrAppendURLC(char* url, AjPStr* string){
  if(!gStrAppendURLS(ajStrNewC(url), string))
    {
      return ajFalse;
    }

  return ajTrue;
}
예제 #12
0
파일: gfile.c 프로젝트: ktnyt/GEMBASSY
AjBool gFileOutURLC(char* url, AjPFile* outf){
  if(!gFileOutURLS(ajStrNewC(url), outf))
    {
      return ajFalse;
    }

  return ajTrue;
}
예제 #13
0
파일: gfile.c 프로젝트: ktnyt/GEMBASSY
AjBool gFilebuffURLC(char* url, AjPFilebuff* buff){
  gFilebuffURLS(ajStrNewC(url), buff);

  if(!*buff)
    return ajFalse;

  return ajTrue;
}
예제 #14
0
static AjBool dbxflat_ParseEmbl(EmbPBtreeEntry entry, AjPFile inf)
{
    AjPStr line = NULL;
    ajlong pos  = 0L;
    
    line = ajStrNewC("");
    
    while(!ajStrPrefixC(line,"//"))
    {
	pos = ajFileResetPos(inf);
	
	if(!ajReadlineTrim(inf,&line))
	{
	    ajStrDel(&line);
	    return ajFalse;
	}
	if(ajStrPrefixC(line,"ID"))
	{
	    entry->fpos = pos;
	    ajFmtScanS(line,"%*S%S",&entry->id);
	    ajStrTrimEndC(&entry->id, ";");
/*
	    ++global;
	    printf("%d. %s\n",global,ajStrGetPtr(entry->id));
*/
	    if(entry->do_sv)
		embBtreeEmblSV(line,entry->sv);
	}


	if(entry->do_sv)
	    if(ajStrPrefixC(line,"SV") ||
	       ajStrPrefixC(line,"IV"))	/* emblcds database format */
		embBtreeEmblAC(line,entry->sv);

	if(entry->do_accession)
	    if(ajStrPrefixC(line,"AC") ||
	       ajStrPrefixC(line,"PA"))	/* emblcds database format */
		embBtreeEmblAC(line,entry->ac);
	
	if(entry->do_keyword)
	    if(ajStrPrefixC(line,"KW"))
		embBtreeEmblKW(line,entry->kw,entry->kwlen);

	if(entry->do_description)
	    if(ajStrPrefixC(line,"DE"))
		embBtreeEmblDE(line,entry->de,entry->delen);

	if(entry->do_taxonomy)
	    if(ajStrPrefixC(line,"OC") || ajStrPrefixC(line,"OS"))
		embBtreeEmblTX(line,entry->tx,entry->txlen);
    }
    

    ajStrDel(&line);
    
    return ajTrue;
}
예제 #15
0
static AjBool dbxflat_ParseEmbl(EmbPBtreeEntry entry, AjPFile inf)
{
    AjPStr line = NULL;
    ajlong pos  = 0L;
    
    line = ajStrNewC("");
    
    while(!ajStrPrefixC(line,"//"))
    {
	pos = ajFileResetPos(inf);
	
	if(!ajReadlineTrim(inf,&line))
	{
	    ajStrDel(&line);
	    return ajFalse;
	}
	if(ajStrPrefixC(line,"ID"))
	{
	    entry->fpos = pos;
	    ajFmtScanS(line,"%*S%S",&entry->id);
	    ajStrTrimEndC(&entry->id, ";");
/*
	    ++global;
	    printf("%d. %s\n",global,ajStrGetPtr(entry->id));
*/
	    if(svfield)
		embBtreeEmblSV(line,svfield->data);
	}


	if(svfield)
	    if(ajStrPrefixC(line,"SV") ||
	       ajStrPrefixC(line,"IV"))	/* emblcds database format */
		embBtreeEmblAC(line,svfield->data);

	if(accfield)
	    if(ajStrPrefixC(line,"AC") ||
	       ajStrPrefixC(line,"PA"))	/* emblcds database format */
		embBtreeEmblAC(line,accfield->data);
	
	if(keyfield)
	    if(ajStrPrefixC(line,"KW"))
		embBtreeEmblKW(line,keyfield->data,keyfield->len);

	if(desfield)
	    if(ajStrPrefixC(line,"DE"))
		embBtreeEmblDE(line,desfield->data,desfield->len);

	if(orgfield)
	    if(ajStrPrefixC(line,"OC") || ajStrPrefixC(line,"OS"))
		embBtreeEmblTX(line,orgfield->data,orgfield->len);
    }
    

    ajStrDel(&line);
    
    return ajTrue;
}
예제 #16
0
파일: ghttp.c 프로젝트: ktnyt/GEMBASSY
AjPFilebuff gHttpPostFileCS(char* url, AjPStr filename)
{
  AjPFilebuff buff = NULL;

  buff = gHttpPostFileSS(ajStrNewC(url), filename);

  if(!buff)
    return NULL;

  return buff;
}
예제 #17
0
static void jaspextract_writematrixfile(const AjPTable mtable,
                                        const AjPStr directory)
{
    AjPStr wild   = NULL;
    AjPList flist = NULL;
    AjPStr key    = NULL;
    AjPStr fname  = NULL;
    AjPStr dest   = NULL;
    const AjPStr value  = NULL;
    
    AjPFile outf = NULL;
    
    const char *p = NULL;
    char *q = NULL;
    
    wild = ajStrNewC("*.pfm");
    flist = ajListNew();
    key   = ajStrNew();
    dest  = ajStrNew();
    
    ajFmtPrintS(&dest,"%S%c%s",directory,SLASH_CHAR,MATRIXFILE);
    
    outf = ajFileNewOutNameS(dest);
    if(!outf)
        ajFatal("Cannot open output file %S",dest);

    ajFilelistAddPathWild(flist, directory, wild);

    while(ajListPop(flist,(void**)&fname))
    {
        ajFilenameTrimPath(&fname);        

        p = ajStrGetPtr(fname);
        q = strrchr(p,(int)'.');
        ajStrAssignSubC(&key,p,0,q-p-1);
        
        value = ajTableFetchS(mtable, key);

        if(value)
            ajFmtPrintF(outf,"%S",value);

        ajStrDel(&fname);
    }
    
        
    ajFileClose(&outf);
    
    ajStrDel(&wild);
    ajStrDel(&dest);
    ajStrDel(&key);
    ajListFree(&flist);
    
    return;
}
예제 #18
0
static AjPTable emira_makepreftab(void)
{
    AjPTable ret = NULL;
    ajuint i;
    AjPStr key = NULL;
    AjPStr value = NULL;
    
    ret = ajTablestrNewLen(MIRATAB_GUESS);

    i = 0;
    while(miraprefix[i].qname)
    {
	key   = ajStrNewC(miraprefix[i].qname);
	value = ajStrNewC(miraprefix[i].prefix);

	ajTablePut(ret, (void *)key, (void *)value);
	++i;
    }

    return ret;
}
예제 #19
0
const AjPStr ajMatrixfGetName(const AjPMatrixf thys)
{
    static AjPStr emptystr = NULL;

    if(!thys)
    {
	emptystr = ajStrNewC("");

	return emptystr;
    }

    return thys->Name;
}
예제 #20
0
파일: ajsoap.c 프로젝트: ICO2S/emboss
axis2_svc_client_t* ajSoapAxis2GetClient(const axutil_env_t *env,
                                         const axis2_char_t *address)
{
    AjPStr home = NULL;

    axis2_endpoint_ref_t* endpoint_ref = NULL;
    axis2_options_t* options           = NULL;
    axis2_svc_client_t* svc_client     = NULL;

    options      = axis2_options_create(env);

    endpoint_ref = axis2_endpoint_ref_create(env, address);

    axis2_options_set_soap_version(options, env, AXIOM_SOAP11);
    axis2_options_set_to(options, env, endpoint_ref);

    /* Set up axis2c location.
    ** This is either from:
    ** EMBOSS_AXIS2C_HOME: Used by mEMBOSS as axis2c files are
    **               provided by the setup and there could
    **               be confusion with an existing axis2c
    **               installation otherwise.
    ** axis2C_HOME: The location specified during the
    **              EMBOSS configuration.
    **
    ** The client uses this information to pick up the
    ** libraries, modules and axis2.xml file
    */

    if(!ajNamGetValueC("AXIS2C_HOME",&home))
        home = ajStrNewC(axis2C_HOME);

    /* Create service client */
    svc_client = axis2_svc_client_create(env, ajStrGetPtr(home));

    if (!svc_client)
    {
	ajErr("Error creating webservice client: %s",
	      AXIS2_ERROR_GET_MESSAGE(env->error));
	return NULL;
    }

    axis2_svc_client_set_options(svc_client, env, options);
    axis2_svc_client_engage_module(svc_client, env, AXIS2_MODULE_ADDRESSING);

    ajStrDel(&home);

    return svc_client;
}
예제 #21
0
int main(int argc, char *argv[])
{
  embInitPV("gseqinfo", argc, argv, "GEMBASSY", "1.0.1");

  struct soap soap;

  AjPSeqall seqall;
  AjPSeq    seq;
  AjPStr    inseq  = NULL;
  AjPStr    seqid  = NULL;
  AjPStr    tmp    = NULL;
  AjPStr    parse  = NULL;
  AjPStr    numA   = NULL;
  AjPStr    numT   = NULL;
  AjPStr    numG   = NULL;
  AjPStr    numC   = NULL;
  AjPStrTok handle = NULL;

  ajint n;

  char *in0;
  char *result;

  AjBool  show = 0;
  AjPFile outf = NULL;

  seqall = ajAcdGetSeqall("sequence");

  outf = ajAcdGetOutfile("outfile");

  while(ajSeqallNext(seqall, &seq))
    {

      soap_init(&soap);

      inseq = NULL;

      ajStrAppendC(&inseq, ">");
      ajStrAppendS(&inseq, ajSeqGetNameS(seq));
      ajStrAppendC(&inseq, "\n");
      ajStrAppendS(&inseq, ajSeqGetSeqS(seq));

      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      in0 = ajCharNewS(inseq);

      if(soap_call_ns1__seqinfo(
			       &soap,
                                NULL,
                                NULL,
			        in0,
                               &result
			       ) == SOAP_OK)
        {
          tmp = ajStrNewC(result);

          ajStrExchangeCC(&tmp, "<", "\n");
          ajStrExchangeCC(&tmp, ">", "\n");

          handle = ajStrTokenNewC(tmp, "\n");

          while(ajStrTokenNextParse(handle, &parse))
            {
              if(ajStrIsInt(parse))
                if(!numA)
                  numA = ajStrNewS(parse);
                else if(!numT)
                  numT = ajStrNewS(parse);
                else if(!numG)
                  numG = ajStrNewS(parse);
                else if(!numC)
                  numC = ajStrNewS(parse);
            }
          if(show)
            ajFmtPrint("Sequence: %S A: %S T: %S G: %S C: %S\n",
                       seqid, numA, numT, numG, numC);
          else
            ajFmtPrintF(outf, "Sequence: %S A: %S T: %S G: %S C: %S\n",
                        seqid, numA, numT, numG, numC);
        }
      else
        {
          soap_print_fault(&soap, stderr);
        }

      soap_destroy(&soap);
      soap_end(&soap);
      soap_done(&soap);

      AJFREE(in0);

      ajStrDel(&inseq);
  }

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&seqid);

  embExit();

  return 0;
}
예제 #22
0
int main(int argc, char *argv[])
{
  embInitPV("gp2", argc, argv, "GEMBASSY", "1.0.1");

  AjPSeqall seqall;
  AjPSeq    seq;
  AjPStr    inseq = NULL;

  AjBool accid  = ajFalse;
  AjPStr restid = NULL;
  AjPStr seqid  = NULL;

  AjPStr base = NULL;
  AjPStr url  = NULL;

  AjPFile tmpfile = NULL;
  AjPStr  tmpname = NULL;

  AjPFile outf = NULL;

  seqall = ajAcdGetSeqall("sequence");
  accid  = ajAcdGetBoolean("accid");
  outf   = ajAcdGetOutfile("outfile");

  base = ajStrNewC("rest.g-language.org");

  gAssignUniqueName(&tmpname);

  while(ajSeqallNext(seqall, &seq))
    {
      inseq = NULL;

      if(!accid)
        {
          if(gFormatGenbank(seq, &inseq))
            {
              tmpfile = ajFileNewOutNameS(tmpname);
              if(!tmpfile)
                {
                  ajDie("Output file (%S) open error\n", tmpname);
                }
              ajFmtPrintF(tmpfile, "%S", inseq);
              ajFileClose(&tmpfile);
              ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
              gFilePostSS(url, tmpname, &restid);
              ajStrDel(&url);
              ajSysFileUnlinkS(tmpname);
            }
          else
            {
              ajDie("Sequence does not have features\n"
                    "Proceeding with sequence accession ID\n");
              accid = ajTrue;
            }
        }

      if(accid)
        {
          ajStrAssignS(&restid, ajSeqGetAccS(seq));
          if(!ajStrGetLen(restid))
            {
              ajStrAssignS(&restid, ajSeqGetNameS(seq));
            }
          if(!ajStrGetLen(restid))
            {
              ajDie("No valid header information\n");
            }
        }

      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      url = ajStrNew();

      ajFmtPrintS(&url, "http://%S/%S/P2/output=f/tag=gene", base, restid);

      ajFmtPrintF(outf, "Sequence: %S\n", seqid);
      if(!gFileOutURLS(url, &outf))
        {
          ajDie("Failed to download result from:\n%S\n", url);
        }

      ajStrDel(&url);
      ajStrDel(&inseq);
    }

  ajFileClose(&outf);

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&seqid);

  embExit();

  return 0;
}
int main(int argc, char *argv[])
{
  embInitPV("gbaseinformationcontent", argc, argv, "GEMBASSY", "1.0.1");

  AjPSeqall seqall;
  AjPSeq    seq;
  AjPStr    inseq = NULL;

  AjPStr position   = 0;
  ajint  PatLen     = 0;
  ajint  upstream   = 0;
  ajint  downstream = 0;

  AjBool accid  = ajFalse;
  AjPStr restid = NULL;
  AjPStr seqid  = NULL;

  AjPStr base = NULL;
  AjPStr url  = NULL;

  AjPFile tmpfile = NULL;
  AjPStr  tmpname = NULL;

  AjBool      plot = 0;
  AjPFile     outf = NULL;
  AjPFilebuff buff = NULL;
  AjPGraph    mult = NULL;

  gPlotParams gpp;
  AjPStr      title = NULL;

  seqall     = ajAcdGetSeqall("sequence");
  position   = ajAcdGetSelectSingle("position");
  PatLen     = ajAcdGetInt("patlen");
  upstream   = ajAcdGetInt("upstream");
  downstream = ajAcdGetInt("downstream");
  accid      = ajAcdGetBoolean("accid");

  plot = ajAcdGetToggle("plot");
  outf = ajAcdGetOutfile("outfile");
  mult = ajAcdGetGraphxy("graph");

  base = ajStrNewC("rest.g-language.org");

  gAssignUniqueName(&tmpname);

  while(ajSeqallNext(seqall, &seq))
    {
      inseq = NULL;

      if(!accid)
        {
          if(gFormatGenbank(seq, &inseq))
            {
              gAssignUniqueName(&tmpname);

              tmpfile = ajFileNewOutNameS(tmpname);

              if(!tmpfile)
                {
                  ajFmtError("Output file (%S) open error\n", tmpname);
                  embExitBad();
                }

              ajFmtPrintF(tmpfile, "%S", inseq);
              ajFileClose(&tmpfile);
              ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
              gFilePostSS(url, tmpname, &restid);
              ajStrDel(&url);
              ajSysFileUnlinkS(tmpname);
            }
          else
            {
              ajFmtError("Sequence does not have features\n"
                         "Proceeding with sequence accession ID\n");
              accid = ajTrue;
            }
        }

      if(accid)
        {
          ajStrAssignS(&seqid, ajSeqGetAccS(seq));

          if(!ajStrGetLen(seqid))
            {
              ajStrAssignS(&seqid, ajSeqGetNameS(seq));
            }

          if(!ajStrGetLen(seqid))
            {
              ajFmtError("No valid header information\n");
              embExitBad();
            }

          ajStrAssignS(&restid, seqid);
        }

      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      url = ajStrNew();

      ajFmtPrintS(&url, "http://%S/%S/base_information_content/position=%S/"
                  "PatLen=%d/upstream=%d/downstream=%d/output=f/tag=gene",
                  base, restid, position, PatLen, upstream, downstream);

      if(plot)
        {
          title = ajStrNew();

          ajStrAppendC(&title, argv[0]);
          ajStrAppendC(&title, " of ");
          ajStrAppendS(&title, seqid);

          gpp.title = ajStrNewS(title);
          gpp.xlab = ajStrNewC("position");
          gpp.ylab = ajStrNewC("information content");

          if(!gFilebuffURLS(url, &buff))
            {
              ajDie("File downloading error from:\n%S\n", url);
            }

          if(!gPlotFilebuff(buff, mult, &gpp))
            {
              ajDie("Error in plotting\n");
            }

          AJFREE(gpp.title);
          AJFREE(gpp.xlab);
          AJFREE(gpp.ylab);
          ajStrDel(&title);
          ajFilebuffDel(&buff);
        }
      else
        {
          ajFmtPrintF(outf, "Sequence: %S\n", seqid);
          if(!gFileOutURLS(url, &outf))
            {
              ajDie("File downloading error from:\n%S\n", url);
            }
        }
    }

  ajFileClose(&outf);

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&seqid);

  ajStrDel(&position);

  embExit();

  return 0;
}
예제 #24
0
int main(int argc, char **argv) {

  embInitPV("kdnapenny", argc, argv, "KBWS", "1.0.8");

  struct soap soap;
  char* jobid;
  char* result;

  AjPSeqall  seqall;
  AjPSeq     seq;
  AjPFile    outf;
  AjPStr     substr;
  AjPStr     inseq = NULL;

  seqall = ajAcdGetSeqall("seqall");
  outf   = ajAcdGetOutfile("outfile");

  AjPStr    tmp         = NULL;
  AjPStr    tmpFileName = NULL;
  AjPSeqout fil_file;
  AjPStr    line        = NULL;
  AjPStr    sizestr     = NULL;
  ajint     thissize    = 0;
  ajint     nb          = 0;
  AjBool    are_prot    = ajFalse;
  ajint     size        = 0;
  AjPFile   infile;

  tmp = ajStrNewC("fasta");

  fil_file    = ajSeqoutNew();
  tmpFileName = getUniqueFileName();

  if( !ajSeqoutOpenFilename(fil_file, tmpFileName) ) {
    embExitBad();
  }

  ajSeqoutSetFormatS(fil_file, tmp);

  while (ajSeqallNext(seqall, &seq)) {
    if (!nb) {
      are_prot  = ajSeqIsProt(seq);
    }
    ajSeqoutWriteSeq(fil_file, seq);
    ++nb;
  }
  ajSeqoutClose(fil_file);
  ajSeqoutDel(&fil_file);

  if (nb < 2) {
    ajFatal("Multiple alignments need at least two sequences");
  }

  infile = ajFileNewInNameS(tmpFileName);

  while (ajReadline(infile, &line)) {
    ajStrAppendS(&inseq,line);
    ajStrAppendC(&inseq,"\n");
  }

  soap_init(&soap);

  char* in0;
  in0 = ajCharNewS(inseq);
  if ( soap_call_ns1__runDnapenny( &soap, NULL, NULL, in0, &jobid ) == SOAP_OK ) {
    fprintf(stderr,"Jobid: %s\n",jobid);
  } else {
    soap_print_fault(&soap, stderr);
  }

  int check = 0;
  while ( check == 0 ) {
    if ( soap_call_ns1__checkStatus( &soap, NULL, NULL, jobid,  &check ) == SOAP_OK ) {
      fprintf(stderr,"*");
    } else {
      soap_print_fault(&soap, stderr);
    }
    sleep(3);
  }
  fprintf(stderr, "\n");

  if ( soap_call_ns1__getResult( &soap, NULL, NULL, jobid,  &result ) == SOAP_OK ) {
    substr = ajStrNewC(result);
    ajFmtPrintF(outf,"%S\n",substr);
  } else {
    soap_print_fault(&soap, stderr);
  }

  ajSysFileUnlinkS(tmpFileName);

  soap_destroy(&soap);
  soap_end(&soap);
  soap_done(&soap);

  ajFileClose(&outf);
  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&substr);

  embExit();

  return 0;
}
예제 #25
0
int main(int argc, char *argv[])
{
    embInitPV("ggcskew", argc, argv, "GEMBASSY", "1.0.3");

    AjPSeqall seqall;
    AjPSeq    seq;
    AjPStr    inseq      = NULL;

    AjBool accid  = ajFalse;
    AjPStr restid = NULL;
    AjPStr seqid  = NULL;

    AjPStr base = NULL;
    AjPStr url  = NULL;

    AjPStr    tmpname = NULL;
    AjPSeqout tmpout  = NULL;

    ajint	 window     = 0;
    ajint	 slide      = 0;
    AjBool cumulative = 0;
    AjBool at         = 0;
    AjBool purine     = 0;
    AjBool keto       = 0;

    AjBool      plot = 0;
    AjPFile     outf = NULL;
    AjPFilebuff buff = NULL;
    AjPGraph    mult = NULL;

    gPlotParams gpp;
    AjPStr      title = NULL;

    seqall     = ajAcdGetSeqall("sequence");
    window     = ajAcdGetInt("window");
    slide      = ajAcdGetInt("slide");
    cumulative = ajAcdGetBoolean("cumulative");
    at         = ajAcdGetBoolean("at");
    purine     = ajAcdGetBoolean("purine");
    keto       = ajAcdGetBoolean("keto");

    plot = ajAcdGetToggle("plot");
    outf = ajAcdGetOutfile("outfile");
    mult = ajAcdGetGraphxy("graph");

    base = ajStrNewC("rest.g-language.org");

    gAssignUniqueName(&tmpname);
    ajStrAppendC(&tmpname, ".fasta");

    while(ajSeqallNext(seqall, &seq))
    {
        tmpout = ajSeqoutNew();

        if(!ajSeqoutOpenFilename(tmpout, tmpname))
        {
            embExitBad();
        }

        ajSeqoutSetFormatS(tmpout,ajStrNewC("fasta"));
        ajSeqoutWriteSeq(tmpout, seq);
        ajSeqoutClose(tmpout);
        ajSeqoutDel(&tmpout);

        ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
        gFilePostSS(url, tmpname, &restid);
        ajStrDel(&url);
        ajSysFileUnlinkS(tmpname);

        ajStrAssignS(&seqid, ajSeqGetAccS(seq));

        if(ajStrGetLen(seqid) == 0)
        {
            ajStrAssignS(&seqid, ajSeqGetNameS(seq));
        }

        if(ajStrGetLen(seqid) == 0)
        {
            ajWarn("No valid header information\n");
        }

        url = ajStrNew();

        ajFmtPrintS(&url, "http://%S/%S/gcskew/window=%d/slide=%d/cumulative=%d/"
                    "at=%d/purine=%d/keto=%d/output=f/", base, restid, window,
                    slide, cumulative, at, purine, keto);

        if(plot)
        {
            title = ajStrNew();

            ajStrAppendC(&title, argv[0]);
            ajStrAppendC(&title, " of ");
            ajStrAppendS(&title, seqid);

            gpp.title = ajStrNewS(title);
            gpp.xlab = ajStrNewC("location");
            gpp.ylab = ajStrNewC("GC skew");

            if(!gFilebuffURLS(url, &buff))
            {
                ajDie("File downloading error from:\n%S\n", url);
            }

            if(!gPlotFilebuff(buff, mult, &gpp))
            {
                ajDie("Error in plotting\n");
            }

            AJFREE(gpp.title);
            AJFREE(gpp.xlab);
            AJFREE(gpp.ylab);
            ajStrDel(&title);
            ajFilebuffDel(&buff);
        }
        else
        {
            ajFmtPrintF(outf, "Sequence: %S\n", seqid);
            if(!gFileOutURLS(url, &outf))
            {
                ajDie("File downloading error from:\n%S\n", url);
            }
        }
        ajStrDel(&url);
        ajStrDel(&restid);
        ajStrDel(&seqid);
    }

    ajFileClose(&outf);

    ajSeqallDel(&seqall);
    ajSeqDel(&seq);
    ajStrDel(&base);

    embExit();

    return 0;
}
예제 #26
0
    int main(int argc, char **argv) {

    embInitPV("kmafft", argc, argv, "KBWS", "1.0.8");

    struct soap soap;
    struct ns1__mafftInputParams params;
    char* jobid;
    char* result;

    AjPSeqall  seqall;
    AjPSeq     seq;
    AjPFile    outf;
    AjPStr     substr;
    AjPStr     inseq = NULL;
    AjPStr     strategy;
    AjPStr     outorder;
    float      op;
    float      ep;
    AjPStr     scorematrix;
    AjBool     homologs;
    AjBool     showhomologs;
    float      threshold;
    AjPStr     referenceseq;
    AjPStr     harrplot;

    strategy     =      ajAcdGetString("strategy");
    outorder     =      ajAcdGetString("outorder");
    op           =      ajAcdGetFloat("op");
    ep           =      ajAcdGetFloat("ep");
    scorematrix  =      ajAcdGetString("scorematrix");
    homologs     =      ajAcdGetBoolean("homologs");
    showhomologs =      ajAcdGetBoolean("showhomologs");
    threshold    =      ajAcdGetFloat("threshold");
    referenceseq =      ajAcdGetString("referenceseq");
    harrplot     =      ajAcdGetString("harrplot");

    seqall = ajAcdGetSeqall("seqall");
    outf   = ajAcdGetOutfile("outfile");

    params.strategy = ajCharNewS(strategy);
    params.outorder = ajCharNewS(outorder);
    params.op = op;
    params.ep = ep;
    params.scorematrix = ajCharNewS(scorematrix);
    if (homologs) {
      params.homologs = xsd__boolean__true_;
    } else {
      params.homologs = xsd__boolean__false_;
    }
    if (showhomologs) {
      params.showhomologs = xsd__boolean__true_;
    } else {
      params.showhomologs = xsd__boolean__false_;
    }
    params.threshold = threshold;
    params.referenceseq = ajCharNewS(referenceseq);
    params.harrplot = ajCharNewS(harrplot);

    AjPStr     tmp         = NULL;
    AjPStr     tmpFileName = NULL;
    AjPSeqout  fil_file;
    AjPStr     line        = NULL; /* if "AjPStr line; -> ajReadline is not success!" */
    AjPStr     sizestr     = NULL;
    ajint      thissize;

    ajint      nb       = 0;
    AjBool     are_prot = ajFalse;
    ajint      size     = 0;
    AjPFile    infile;

    tmp = ajStrNewC("fasta");

    fil_file = ajSeqoutNew();
    tmpFileName = getUniqueFileName();

    if( !ajSeqoutOpenFilename(fil_file, tmpFileName) ) {
        embExitBad();
    }

    ajSeqoutSetFormatS(fil_file, tmp);

    while (ajSeqallNext(seqall, &seq)) {
      if (!nb) {
        are_prot  = ajSeqIsProt(seq);
    }
      ajSeqoutWriteSeq(fil_file, seq);
      ++nb;
    }
    ajSeqoutClose(fil_file);
    ajSeqoutDel(&fil_file);

    if (nb < 2) {
        ajFatal("Multiple alignments need at least two sequences");
    }

    infile = ajFileNewInNameS(tmpFileName);

    while (ajReadline(infile, &line)) {
      ajStrAppendS(&inseq,line);
      ajStrAppendC(&inseq,"\n");
    }

    soap_init(&soap);

    char* in0;
    in0 = ajCharNewS(inseq);
    if ( soap_call_ns1__runMafft( &soap, NULL, NULL, in0, &params, &jobid ) == SOAP_OK ) {
      fprintf(stderr,"Jobid: %s\n",jobid);
    } else {
      soap_print_fault(&soap, stderr);
    }

    int check = 0;
    while ( check == 0 ) {
      if ( soap_call_ns1__checkStatus( &soap, NULL, NULL, jobid,  &check ) == SOAP_OK ) {
        fprintf(stderr,"*");
      } else {
        soap_print_fault(&soap, stderr);
      }
      sleep(3);
    }

    fprintf(stderr,"\n");

    if ( soap_call_ns1__getResult( &soap, NULL, NULL, jobid,  &result ) == SOAP_OK ) {
      substr = ajStrNewC(result);
      ajFmtPrintF(outf,"%S\n",substr);
    } else {
      soap_print_fault(&soap, stderr);
    }

    ajSysFileUnlinkS(tmpFileName);

    soap_destroy(&soap);
    soap_end(&soap);
    soap_done(&soap);

    ajFileClose(&outf);

    ajSeqallDel(&seqall);
    ajSeqDel(&seq);
    ajStrDel(&substr);

    embExit();

    return 0;
}
예제 #27
0
int main(int argc, char *argv[])
{
  embInitPV("gdeltagcskew", argc, argv, "GEMBASSY", "1.0.3");

  AjPSeqall seqall;
  AjPSeq    seq;
  AjPStr    inseq = NULL;

  AjBool at     = 0;
  AjBool purine = 0;
  AjBool keto   = 0;
  AjPStr method = 0;

  AjBool accid  = ajFalse;
  AjPStr restid = NULL;
  AjPStr seqid  = NULL;

  AjPStr base = NULL;
  AjPStr url  = NULL;

  AjPFile tmpfile = NULL;
  AjPStr  tmpname = NULL;
  AjPFilebuff tmp = NULL;

  AjPStr line = NULL;

  AjPFile outf = NULL;

  seqall = ajAcdGetSeqall("sequence");
  at     = ajAcdGetBoolean("at");
  purine = ajAcdGetBoolean("purine");
  keto   = ajAcdGetBoolean("keto");
  method = ajAcdGetSelectSingle("method");
  accid  = ajAcdGetBoolean("accid");
  outf   = ajAcdGetOutfile("outfile");

  base = ajStrNewC("rest.g-language.org");

  gAssignUniqueName(&tmpname);

  while(ajSeqallNext(seqall, &seq))
    {
      inseq = NULL;

      if(!accid)
        {
          if(gFormatGenbank(seq, &inseq))
            {
              tmpfile = ajFileNewOutNameS(tmpname);
              if(!tmpfile)
                {
                  ajDie("Output file (%S) open error\n", tmpname);
                }
              ajFmtPrintF(tmpfile, "%S", inseq);
              ajFileClose(&tmpfile);
              ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
              gFilePostSS(url, tmpname, &restid);
              ajStrDel(&url);
              ajSysFileUnlinkS(tmpname);
            }
          else
            {
              ajWarn("Sequence does not have features\n"
                     "Proceeding with sequence accession ID\n");
              accid = ajTrue;
            }
        }

      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      if(ajStrGetLen(seqid) == 0)
        {
          ajStrAssignS(&seqid, ajSeqGetNameS(seq));
        }

      if(ajStrGetLen(seqid) == 0)
        {
          ajWarn("No valid header information\n");
        }

      if(accid)
        {
          ajStrAssignS(&restid, seqid);
          if(ajStrGetLen(seqid) == 0)
            {
              ajDie("Cannot proceed without header with -accid\n");
            }

          if(!gValID(seqid))
            {
              ajDie("Invalid accession ID:%S, exiting\n", seqid);
            }
        }

      url = ajStrNew();

      ajFmtPrintS(&url, "http://%S/%S/delta_gcskew/", base, restid);

      if(!gFilebuffURLS(url, &tmp))
        {
          ajDie("Failed to download result from:\n%S\n", url);
        }

      ajBuffreadLine(tmp, &line);

      ajStrRemoveSetC(&line, "\n");

      ajFmtPrintF(outf, "Sequence: %S DELTA-GCskew %S\n", seqid, line);

      ajStrDel(&url);
      ajStrDel(&restid);
      ajStrDel(&seqid);
      ajStrDel(&inseq);
    }

  ajFileClose(&outf);

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&base);

  embExit();

  return 0;
}
예제 #28
0
int main(int argc, char **argv)
{
    AjPSeqall seqall;
    AjPSeq seq    = NULL;
    AjPFile outf  = NULL;
    AjPStr strand = NULL;
    AjPStr substr = NULL;
    AjPStr bases  = NULL;

    ajint begin;
    ajint end;
    ajint len;

    ajint minlen;
    float minobsexp;
    float minpc;

    ajint window;
    ajint shift;
    ajint plotstart;
    ajint plotend;

    float  *xypc   = NULL;
    float  *obsexp = NULL;
    AjBool *thresh = NULL;
    float  obsexpmax;

    ajint i;
    ajint maxarr;


    embInit("newcpgreport",argc,argv);

    seqall    = ajAcdGetSeqall("sequence");
    window    = ajAcdGetInt("window");
    shift     = ajAcdGetInt("shift");
    outf      = ajAcdGetOutfile("outfile");
    minobsexp = ajAcdGetFloat("minoe");
    minlen    = ajAcdGetInt("minlen");
    minpc     = ajAcdGetFloat("minpc");

    substr = ajStrNew();
    bases  = ajStrNewC("CG");
    maxarr = 0;

    while(ajSeqallNext(seqall, &seq))
    {
	begin = ajSeqallGetseqBegin(seqall);
	end   = ajSeqallGetseqEnd(seqall);
	strand = ajSeqGetSeqCopyS(seq);
	ajStrFmtUpper(&strand);

	ajStrAssignSubC(&substr,ajStrGetPtr(strand),--begin,--end);
	len=ajStrGetLen(substr);

	if(len > maxarr)
	{
	    AJCRESIZE(obsexp, len);
	    AJCRESIZE(thresh, len);
	    AJCRESIZE(xypc, len);
	    maxarr = len;
	}
	for(i=0;i<len;++i)
	    obsexp[i]=xypc[i]=0.0;


	newcpgreport_findbases(substr, len, window, shift, obsexp,
			       xypc, bases, &obsexpmax, &plotstart, &plotend);

	newcpgreport_identify(outf, obsexp, xypc, thresh, 0, len, shift,
			      ajStrGetPtr(bases), ajSeqGetNameC(seq), minlen,
			      minobsexp, minpc, ajStrGetPtr(strand));

	ajStrDel(&strand);
    }

    ajStrDel(&bases);

    ajSeqDel(&seq);
    ajStrDel(&substr);
    ajFileClose(&outf);

    AJFREE(obsexp);
    AJFREE(thresh);
    AJFREE(xypc);

    ajSeqallDel(&seqall);

    embExit();

    return 0;
}
예제 #29
0
AjBool ajDebugTest(const char* token)
{
    AjPStr filename = NULL;
    const char* debugtestname = ".debugtest";
    char* ctoken = NULL;
    AjPStr line = NULL;
    AjPStr strtoken = NULL;
    AjPStr rest = NULL;
    static ajint depth    = 0;

    struct 
    {
        ajuint count;
        ajuint max;
    } *stats;
    
    if(depth)
        return ajFalse;

    depth++;

    if(!messDebugTestInit)
    {
        filename = ajStrNewC(debugtestname);

        if(ajFilenameExists(filename))
        {
            messDebugTestFile = ajFileNewInNameS(filename);
        }
        else
        {
            ajFmtPrintS(&filename, "%s%s%s",
                        getenv("HOME"), SLASH_STRING, debugtestname); 
            if(ajFilenameExists(filename))
                messDebugTestFile = ajFileNewInNameS(filename);
        }
        ajStrDel(&filename);

        if(messDebugTestFile) 
        {
            messDebugTestTable = ajTablecharNewLen(256);

            while(ajReadlineTrim(messDebugTestFile, &line))
            {
                if(ajStrExtractFirst(line, &rest, &strtoken))
                {
                    AJNEW0(stats);
                    ctoken = ajCharNewS(strtoken);
                    if(ajStrIsInt(rest))
                        ajStrToUint(rest, &stats->max);
                    else
                        stats->max = UINT_MAX;
                    ajTablePut(messDebugTestTable, ctoken, stats);
                    ctoken = NULL;
                    stats = NULL;
                }
            }

            ajStrDel(&line);
            ajStrDel(&strtoken);
            ajStrDel(&rest);
            ajFileClose(&messDebugTestFile);
        }
        messDebugTestInit = ajTrue;
     }

    depth--;
    
    if(!messDebugTestTable)
        return ajFalse;

    depth++;
    stats = ajTableFetch(messDebugTestTable, token);
    depth--;
    

    if(!stats)
        return ajFalse;

    if(!stats->max)
        return ajTrue;

    if(stats->count++ >= stats->max)
        return ajFalse;

    return ajTrue;
}
예제 #30
0
int main(int argc, char **argv)
{
    AjPList list = NULL;
    AjPSeq seq;
    AjPSeq seq2;
    AjPStr aa0str = 0;
    AjPStr aa1str = 0;
    const char *s1;
    const char *s2;
    char *strret = NULL;
    ajuint i;
    ajuint j;
    ajuint k;
    ajint l;
    ajint abovethresh;
    ajint total;
    ajint starti = 0;
    ajint startj = 0;
    ajint windowsize;
    float thresh;
    AjPGraph graph   = NULL;
    AjPGraph xygraph = NULL;
    float flen1;
    float flen2;
    ajuint len1;
    ajuint len2;

    AjPTime ajtime = NULL;
    time_t tim;
    AjBool boxit=AJTRUE;
    /* Different ticks as they need to be different for x and y due to
       length of string being important on x */
    ajuint acceptableticksx[]=
    {
	1,10,50,100,500,1000,1500,10000,
	500000,1000000,5000000
    };
    ajuint acceptableticks[]=
    {
	1,10,50,100,200,500,1000,2000,5000,10000,15000,
	500000,1000000,5000000
    };
    ajint numbofticks = 10;
    float xmargin;
    float ymargin;
    float ticklen;
    float tickgap;
    float onefifth;
    float k2;
    float max;
    char ptr[10];
    AjPMatrix matrix = NULL;
    ajint** sub;
    AjPSeqCvt cvt;
    AjPStr  subt = NULL;

    ajint b1;
    ajint b2;
    ajint e1;
    ajint e2;
    AjPStr se1;
    AjPStr se2;
    ajint ithresh;
    AjBool stretch;
    PPoint ppt = NULL;
    float xa[1];
    float ya[1];
    AjPGraphdata gdata=NULL;
    AjPStr tit   = NULL;
    AjIList iter = NULL;
    float x1 = 0.;
    float x2 = 0.;
    float y1 = 0.;
    float y2 = 0.;
    ajuint tui;
    
    se1 = ajStrNew();
    se2 = ajStrNew();

    embInit("dotmatcher", argc, argv);
    
    seq        = ajAcdGetSeq("asequence");
    seq2       = ajAcdGetSeq("bsequence");
    stretch    = ajAcdGetToggle("stretch");
    graph      = ajAcdGetGraph("graph");
    xygraph    = ajAcdGetGraphxy("xygraph");
    windowsize = ajAcdGetInt("windowsize");
    ithresh    = ajAcdGetInt("threshold");
    matrix     = ajAcdGetMatrix("matrixfile");
    
    sub = ajMatrixGetMatrix(matrix);
    cvt = ajMatrixGetCvt(matrix);
    
    thresh = (float)ithresh;

    ajtime = ajTimeNew();

    tim = time(0);
    ajTimeSetLocal(ajtime, tim);
    
    b1 = ajSeqGetBegin(seq);
    b2 = ajSeqGetBegin(seq2);
    e1 = ajSeqGetEnd(seq);
    e2 = ajSeqGetEnd(seq2);
    len1 = ajSeqGetLen(seq);
    len2 = ajSeqGetLen(seq2);
    tui   = ajSeqGetLen(seq);
    flen1 = (float) tui;
    tui   = ajSeqGetLen(seq2);
    flen2 = (float) tui;
    
    ajStrAssignSubC(&se1,ajSeqGetSeqC(seq),b1-1,e1-1);
    ajStrAssignSubC(&se2,ajSeqGetSeqC(seq2),b2-1,e2-1);
    ajSeqAssignSeqS(seq,se1);
    ajSeqAssignSeqS(seq2,se2);
    
    
    s1 = ajStrGetPtr(ajSeqGetSeqS(seq));
    s2 = ajStrGetPtr(ajSeqGetSeqS(seq2));
    
    
    aa0str = ajStrNewRes(1+len1); /* length plus trailing blank */
    aa1str = ajStrNewRes(1+len2);
    
    list = ajListNew();
    
    
    for(i=0;i<len1;i++)
	ajStrAppendK(&aa0str,(char)ajSeqcvtGetCodeK(cvt, *s1++));
    
    for(i=0;i<len2;i++)
	ajStrAppendK(&aa1str,(char)ajSeqcvtGetCodeK(cvt, *s2++));
    
    max = (float)len1;
    if(len2 > max)
	max = (float) len2;
    
    xmargin = ymargin = max *(float)0.15;
    ticklen = xmargin*(float)0.1;
    onefifth  = xmargin*(float)0.2;
    
    subt = ajStrNewC((strret=
		      ajFmtString("(windowsize = %d, threshold = %3.2f  %D)",
				  windowsize,thresh,ajtime)));
    
    

    if(!stretch)
    {
	if( ajStrGetLen(ajGraphGetSubtitleS(graph)) <=1)
	    ajGraphSetSubtitleS(graph,subt);

	ajGraphOpenWin(graph, (float)0.0-ymargin,(max*(float)1.35)+ymargin,
		       (float)0.0-xmargin,(float)max+xmargin);

	ajGraphicsDrawposTextAtmid(flen1*(float)0.5,
                                   (float)0.0-(xmargin/(float)2.0),
		       ajGraphGetXlabelC(graph));
	ajGraphicsDrawposTextAtlineJustify((float)0.0-(xmargin*(float)0.75),
                                           flen2*(float)0.5,
			(float)0.0-(xmargin*(float)0.75),flen1,
			ajGraphGetYlabelC(graph),0.5);

	ajGraphicsSetCharscale(0.5);
    }
    
    
    
    s1= ajStrGetPtr(aa0str);
    s2 = ajStrGetPtr(aa1str);
    
    for(j=0; (ajint)j < (ajint)len2-windowsize;j++)
    {
	i =0;
	total = 0;
	abovethresh =0;

	k = j;
	for(l=0;l<windowsize;l++)
	    total = total + sub[(ajint)s1[i++]][(ajint)s2[k++]];

	if(total >= thresh)
	{
	    abovethresh=1;
	    starti = i-windowsize;
	    startj = k-windowsize;
	}

	while(i < len1 && k < len2)
	{
	    total = total - sub[(ajint)s1[i-windowsize]]
		[(ajint)s2[k-windowsize]];
	    total = total + sub[(ajint)s1[i]][(ajint)s2[k]];

	    if(abovethresh)
	    {
		if(total < thresh)
		{
		    abovethresh = 0;
		    /* draw the line */
		    dotmatcher_pushpoint(&list,(float)starti,(float)startj,
					 (float)i-1,(float)k-1,stretch);
		}
	    }
	    else if(total >= thresh)
	    {
		starti = i-windowsize;
		startj = k-windowsize;
		abovethresh= 1;
	    }
	    i++;
	    k++;
	}

	if(abovethresh)
	    /* draw the line */
	    dotmatcher_pushpoint(&list,(float)starti,(float)startj,
				 (float)i-1,(float)k-1,
				 stretch);
    }
    
    for(i=0; (ajint)i < (ajint)len1-windowsize;i++)
    {
	j = 0;
	total = 0;
	abovethresh =0;

	k = i;
	for(l=0;l<windowsize;l++)
	    total = total + sub[(ajint)s1[k++]][(ajint)s2[j++]];

	if(total >= thresh)
	{
	    abovethresh=1;
	    starti = k-windowsize;
	    startj = j-windowsize;
	}

	while(k < len1 && j < len2)
	{
	    total = total - sub[(ajint)s1[k-windowsize]]
		[(ajint)s2[j-windowsize]];
	    total = total + sub[(ajint)s1[k]][(ajint)s2[j]];

	    if(abovethresh)
	    {
		if(total < thresh)
		{
		    abovethresh = 0;
		    /* draw the line */
		    dotmatcher_pushpoint(&list,(float)starti,(float)startj,
					 (float)k-1,(float)j-1,stretch);
		}
	    }
	    else if(total >= thresh)
	    {
		starti = k-windowsize;
		startj = j-windowsize;
		abovethresh= 1;
	    }
	    j++;
	    k++;
	}

	if(abovethresh)
	    /* draw the line */
	    dotmatcher_pushpoint(&list,(float)starti,(float)startj,
				 (float)k-1,(float)j-1,
				 stretch);
    }
    
    if(boxit && !stretch)
    {
	ajGraphicsDrawposRect(0.0,0.0,flen1, flen2);

	i=0;
	while(acceptableticksx[i]*numbofticks < len1)
	    i++;

	if(i<=13)
	    tickgap = (float)acceptableticksx[i];
	else
	    tickgap = (float)acceptableticksx[10];
	ticklen   = xmargin*(float)0.1;
	onefifth  = xmargin*(float)0.2;

	if(len2/len1 > 10 )
	{
	    /* if a lot smaller then just label start and end */
	    ajGraphicsDrawposLine((float)0.0,(float)0.0,(float)0.0,(float)0.0-ticklen);
	    sprintf(ptr,"%d",b1-1);
	    ajGraphicsDrawposTextAtmid((float)0.0,(float)0.0-(onefifth),ptr);

	    ajGraphicsDrawposLine(flen1,(float)0.0,
			flen1,(float)0.0-ticklen);
	    sprintf(ptr,"%d",len1+b1-1);
	    ajGraphicsDrawposTextAtmid(flen1,(float)0.0-(onefifth),ptr);

	}
	else
	    for(k2=0.0;k2<len1;k2+=tickgap)
	    {
		ajGraphicsDrawposLine(k2,(float)0.0,k2,(float)0.0-ticklen);
		sprintf(ptr,"%d",(ajint)k2+b1-1);
		ajGraphicsDrawposTextAtmid(k2,(float)0.0-(onefifth),ptr);
	    }

	i = 0;
	while(acceptableticks[i]*numbofticks < len2)
	    i++;

	tickgap   = (float)acceptableticks[i];
	ticklen   = ymargin*(float)0.01;
	onefifth  = ymargin*(float)0.02;

	if(len1/len2 > 10 )
	{
	    /* if a lot smaller then just label start and end */
	    ajGraphicsDrawposLine((float)0.0,(float)0.0,(float)0.0-ticklen,(float)0.0);
	    sprintf(ptr,"%d",b2-1);
	    ajGraphicsDrawposTextAtend((float)0.0-(onefifth),(float)0.0,ptr);

	    ajGraphicsDrawposLine((float)0.0,flen2,(float)0.0-ticklen,
			flen2);
	    sprintf(ptr,"%d",len2+b2-1);
	    ajGraphicsDrawposTextAtend((float)0.0-(onefifth),flen2,ptr);
	}
	else
	    for(k2=0.0;k2<len2;k2+=tickgap)
	    {
		ajGraphicsDrawposLine((float)0.0,k2,(float)0.0-ticklen,k2);
		sprintf(ptr,"%d",(ajint)k2+b2-1);
		ajGraphicsDrawposTextAtend((float)0.0-(onefifth),k2,ptr);
	    }
    }
    
    
    if(!stretch)
	ajGraphicsClose();
    else			/* the xy graph for -stretch */
    {
	tit = ajStrNew();
	ajFmtPrintS(&tit,"%S",ajGraphGetTitleS(xygraph));


	gdata = ajGraphdataNewI(1);
	xa[0] = (float)b1;
	ya[0] = (float)b2;

	ajGraphSetTitleC(xygraph,ajStrGetPtr(tit));

	ajGraphSetXlabelC(xygraph,ajSeqGetNameC(seq));
	ajGraphSetYlabelC(xygraph,ajSeqGetNameC(seq2));

	ajGraphdataSetTypeC(gdata,"2D Plot Float");
	ajGraphdataSetTitleS(gdata,subt);
	ajGraphdataSetMinmax(gdata,(float)b1,(float)e1,(float)b2,
			       (float)e2);
	ajGraphdataSetTruescale(gdata,(float)b1,(float)e1,(float)b2,
			       (float)e2);
	ajGraphxySetXstartF(xygraph,(float)b1);
	ajGraphxySetXendF(xygraph,(float)e1);
	ajGraphxySetYstartF(xygraph,(float)b2);
	ajGraphxySetYendF(xygraph,(float)e2);

	ajGraphxySetXrangeII(xygraph,b1,e1);
	ajGraphxySetYrangeII(xygraph,b2,e2);


	if(list)
	{
	    iter = ajListIterNewread(list);
	    while((ppt = ajListIterGet(iter)))
	    {
		x1 = ppt->x1+b1-1;
		y1 = ppt->y1+b2-1;
		x2 = ppt->x2+b1-1;
		y2 = ppt->y2+b2-1;
		ajGraphAddLine(xygraph,x1,y1,x2,y2,0);
		AJFREE(ppt);
	    }
	    ajListIterDel(&iter);
	}

	ajGraphdataAddXY(gdata,xa,ya);
	ajGraphDataReplace(xygraph,gdata);


	ajGraphxyDisplay(xygraph,ajFalse);
	ajGraphicsClose();

	ajStrDel(&tit);
    }
    
    
    
    ajListFree(&list);

    ajSeqDel(&seq);
    ajSeqDel(&seq2);
    ajGraphxyDel(&graph);
    ajGraphxyDel(&xygraph);
    ajMatrixDel(&matrix);
    ajTimeDel(&ajtime);
    
    /* deallocate memory */
    ajStrDel(&aa0str);
    ajStrDel(&aa1str);
    ajStrDel(&se1);
    ajStrDel(&se2);
    ajStrDel(&subt);

    AJFREE(strret);			/* created withing ajFmtString */
    
    embExit();

    return 0;
}