static int update_bioseq_if_necessary(GtRegionMapping *rm, GtStr *seqid,
GtError *err)
{
int had_err = 0;
gt_error_check(err);
gt_assert(rm && seqid);
if (!rm->sequence_file || gt_str_cmp(rm->sequence_name, seqid)) {
gt_str_delete(rm->sequence_file);
rm->sequence_file = region_mapping_map(rm, gt_str_get(seqid), err);
if (!rm->sequence_file)
had_err = -1;
else {
if (!rm->sequence_name)
rm->sequence_name = gt_str_new();
else
gt_str_reset(rm->sequence_name);
gt_str_append_str(rm->sequence_name, seqid);
gt_bioseq_delete(rm->bioseq);
rm->bioseq = gt_bioseq_new_str(rm->sequence_file, err);
if (!rm->bioseq)
had_err = -1;
}
}
return had_err;
}
static int select_visitor_region_node(GtNodeVisitor *nv, GtRegionNode *rn,
GT_UNUSED GtError *err)
{
GtSelectVisitor *select_visitor;
gt_error_check(err);
select_visitor = select_visitor_cast(nv);
if (!gt_str_length(select_visitor->seqid) || /* no seqid was specified */
!gt_str_cmp(select_visitor->seqid, /* or seqids are equal */
gt_genome_node_get_seqid((GtGenomeNode*) rn))) {
if (select_visitor->contain_range.start != GT_UNDEF_ULONG) {
GtRange range = gt_genome_node_get_range((GtGenomeNode*) rn);
if (gt_range_overlap(&range, &select_visitor->contain_range)) {
/* an overlapping contain range was defined -> update range */
range.start = MAX(range.start, select_visitor->contain_range.start);
range.end = MIN(range.end, select_visitor->contain_range.end);
gt_genome_node_set_range((GtGenomeNode*) rn, &range);
gt_queue_add(select_visitor->node_buffer, rn);
}
else /* contain range does not overlap with <rn> range -> delete <rn> */
gt_genome_node_delete((GtGenomeNode*) rn);
}
else
gt_queue_add(select_visitor->node_buffer, rn);
}
else
gt_genome_node_delete((GtGenomeNode*) rn);
return 0;
}
static int gt_sort_stream_next(GtNodeStream *ns, GtGenomeNode **gn,
GtError *err)
{
GtSortStream *sort_stream;
GtGenomeNode *node, *eofn;
int had_err = 0;
gt_error_check(err);
sort_stream = gt_sort_stream_cast(ns);
if (!sort_stream->sorted) {
while (!(had_err = gt_node_stream_next(sort_stream->in_stream, &node,
err)) && node) {
if ((eofn = gt_eof_node_try_cast(node)))
gt_genome_node_delete(eofn); /* get rid of EOF nodes */
else
gt_array_add(sort_stream->nodes, node);
}
if (!had_err) {
gt_genome_nodes_sort_stable(sort_stream->nodes);
sort_stream->sorted = true;
}
}
if (!had_err) {
gt_assert(sort_stream->sorted);
if (sort_stream->idx < gt_array_size(sort_stream->nodes)) {
*gn = *(GtGenomeNode**) gt_array_get(sort_stream->nodes,
sort_stream->idx);
sort_stream->idx++;
/* join region nodes with the same sequence ID */
if (gt_region_node_try_cast(*gn)) {
GtRange range_a, range_b;
while (sort_stream->idx < gt_array_size(sort_stream->nodes)) {
node = *(GtGenomeNode**) gt_array_get(sort_stream->nodes,
sort_stream->idx);
if (!gt_region_node_try_cast(node) ||
gt_str_cmp(gt_genome_node_get_seqid(*gn),
gt_genome_node_get_seqid(node))) {
/* the next node is not a region node with the same ID */
break;
}
range_a = gt_genome_node_get_range(*gn);
range_b = gt_genome_node_get_range(node);
range_a = gt_range_join(&range_a, &range_b);
gt_genome_node_set_range(*gn, &range_a);
gt_genome_node_delete(node);
sort_stream->idx++;
}
}
return 0;
}
}
if (!had_err) {
gt_array_reset(sort_stream->nodes);
*gn = NULL;
}
return had_err;
}
void gt_region_node_consolidate(GtRegionNode *rn_a, GtRegionNode *rn_b)
{
GtRange range_a, range_b;
gt_assert(rn_a);
gt_assert(rn_b);
gt_assert(!gt_str_cmp(gt_genome_node_get_seqid((GtGenomeNode*) rn_a),
gt_genome_node_get_seqid((GtGenomeNode*) rn_b)));
range_a = gt_genome_node_get_range((GtGenomeNode*) rn_a);
range_b = gt_genome_node_get_range((GtGenomeNode*) rn_b);
range_a = gt_range_join(&range_a, &range_b);
gt_genome_node_set_range((GtGenomeNode*) rn_a, &range_a);
}
static inline int parse_fastq_block(GtSeqIteratorFastQ *seqit, GtError *err)
{
int had_err = 0;
gt_assert(seqit);
gt_error_check(err);
/* parse @<seqname> */
had_err = parse_fastq_seqname(seqit,
seqit->descbuffer,
GT_FASTQ_BLOCK_START_CHAR,
err);
if (!had_err) {
/* parse sequence */
had_err = parse_fastq_sequence(seqit, err);
gt_fastq_premature_end_check(had_err, seqit);
}
if (!had_err) {
/* parse +[seqname] */
had_err = parse_fastq_seqname(seqit,
seqit->qdescbuffer,
GT_FASTQ_QUAL_SEPARATOR_CHAR,
err);
gt_fastq_premature_end_check(had_err, seqit);
}
if (!had_err
&& gt_str_length(seqit->qdescbuffer)
&& gt_str_cmp(seqit->descbuffer, seqit->qdescbuffer) != 0)
{
gt_error_set(err, "sequence description '%s' is not equal to "
"qualities description '%s' in line %lu",
gt_str_get(seqit->descbuffer),
gt_str_get(seqit->qdescbuffer),
seqit->curline-1);
return -2;
}
if (!had_err) {
/* parse qualities */
had_err = parse_fastq_qualities(seqit, err);
if (gt_str_length(seqit->qualsbuffer)
!= gt_str_length(seqit->sequencebuffer))
{
gt_error_set(err, "lengths of character sequence and qualities "
"sequence differ (%lu <-> %lu)",
gt_str_length(seqit->qualsbuffer),
gt_str_length(seqit->sequencebuffer));
return -2;
}
}
return had_err;
}
static int select_visitor_sequence_node(GtNodeVisitor *nv, GtSequenceNode *sn,
GT_UNUSED GtError *err)
{
GtSelectVisitor *select_visitor;
gt_error_check(err);
select_visitor = select_visitor_cast(nv);
if (!gt_str_length(select_visitor->seqid) || /* no seqid was specified */
!gt_str_cmp(select_visitor->seqid, /* or seqids are equal */
gt_genome_node_get_seqid((GtGenomeNode*) sn))) {
gt_queue_add(select_visitor->node_buffer, sn);
}
else
gt_genome_node_delete((GtGenomeNode*) sn);
return 0;
}
static int inter_feature_in_children(GtFeatureNode *current_feature, void *data,
GT_UNUSED GtError *err)
{
GtInterFeatureVisitor *aiv = (GtInterFeatureVisitor*) data;
GtFeatureNode *inter_node;
GtRange previous_range, current_range, inter_range;
GtStrand previous_strand, /*current_strand, */inter_strand;
GtStr *parent_seqid;
gt_error_check(err);
gt_assert(current_feature);
if (gt_feature_node_has_type(current_feature, aiv->outside_type)) {
if (aiv->previous_feature) {
/* determine inter range */
previous_range = gt_genome_node_get_range((GtGenomeNode*)
aiv->previous_feature);
current_range = gt_genome_node_get_range((GtGenomeNode*) current_feature);
if (previous_range.end >= current_range.start) {
gt_warning("overlapping boundary features " GT_WU "-" GT_WU " and "
GT_WU "-" GT_WU ", " "not placing '%s' inter-feature",
previous_range.start,
previous_range.end,
current_range.start,
current_range.end,
aiv->inter_type);
return 0;
}
if (current_range.start - previous_range.end < 2) {
gt_warning("no space for inter-feature '%s' between " GT_WU " and "
GT_WU,
aiv->inter_type,
previous_range.end,
current_range.start);
return 0;
}
inter_range.start = previous_range.end + 1;
inter_range.end = current_range.start - 1;
/* determine inter strand */
previous_strand = gt_feature_node_get_strand(aiv->previous_feature);
/*current_strand = gt_feature_node_get_strand(current_feature);*/
gt_assert(previous_strand == gt_feature_node_get_strand(current_feature));
inter_strand = previous_strand;
/* determine sequence id */
parent_seqid =
gt_genome_node_get_seqid((GtGenomeNode*) aiv->parent_feature);
gt_assert(!gt_str_cmp(parent_seqid,
gt_genome_node_get_seqid((GtGenomeNode*)
aiv->previous_feature)));
gt_assert(!gt_str_cmp(parent_seqid,
gt_genome_node_get_seqid((GtGenomeNode*)
current_feature)));
/* create inter feature */
inter_node = (GtFeatureNode*)
gt_feature_node_new(parent_seqid, aiv->inter_type,
inter_range.start, inter_range.end,
inter_strand);
gt_feature_node_add_child(aiv->parent_feature, inter_node);
}
aiv->previous_feature = current_feature;
}
return 0;
}
static int select_visitor_feature_node(GtNodeVisitor *nv,
GtFeatureNode *fn,
GT_UNUSED GtError *err)
{
GtSelectVisitor *fv;
bool filter_node = false;
gt_error_check(err);
fv = select_visitor_cast(nv);
fv->current_feature++;
if ((!gt_str_length(fv->seqid) || /* no seqid was specified or seqids are
equal */
!gt_str_cmp(fv->seqid, gt_genome_node_get_seqid((GtGenomeNode*) fn))) &&
(!gt_str_length(fv->source) || /* no source was specified or sources are
equal */
!strcmp(gt_str_get(fv->source), gt_feature_node_get_source(fn)))) {
GtRange range = gt_genome_node_get_range((GtGenomeNode*) fn);
/* enforce maximum gene length */
/* XXX: we (spuriously) assume that genes are always root nodes */
if (fn && gt_feature_node_has_type(fn, gt_ft_gene)) {
if (fv->max_gene_length != GT_UNDEF_ULONG &&
gt_range_length(&range) > fv->max_gene_length) {
filter_node = true;
}
else if (fv->max_gene_num != GT_UNDEF_ULONG &&
fv->gene_num >= fv->max_gene_num) {
filter_node = true;
}
else if (fv->min_gene_score != GT_UNDEF_DOUBLE &&
gt_feature_node_get_score(fn) < fv->min_gene_score) {
filter_node = true;
}
else if (fv->max_gene_score != GT_UNDEF_DOUBLE &&
gt_feature_node_get_score(fn) > fv->max_gene_score) {
filter_node = true;
}
else if (fv->feature_num != GT_UNDEF_ULONG &&
fv->feature_num != fv->current_feature) {
filter_node = true;
}
if (!filter_node)
fv->gene_num++; /* gene passed filter */
}
}
else
filter_node = true;
if (!filter_node)
filter_node = filter_contain_range(fn, fv->contain_range);
if (!filter_node)
filter_node = filter_overlap_range(fn, fv->overlap_range);
if (!filter_node)
filter_node = filter_strand(fn, fv->strand);
if (!filter_node)
filter_node = filter_targetstrand(fn, fv->targetstrand);
if (!filter_node)
filter_node = filter_has_CDS(fn, fv->has_CDS);
if (!filter_node)
filter_node = filter_min_average_ssp(fn, fv->min_average_splice_site_prob);
if (filter_node)
gt_genome_node_delete((GtGenomeNode*) fn);
else
gt_queue_add(fv->node_buffer, fn);
return 0;
}
int gt_block_unit_test(GtError *err)
{
GtRange r1, r2, r_temp, b_range;
GtStrand s;
GtGenomeNode *gn1, *gn2;
GtElement *e1, *e2;
double height;
GtBlock *b;
GtStr *seqid, *caption1, *caption2;
int had_err = 0;
GtStyle *sty;
GtError *testerr;
gt_error_check(err);
seqid = gt_str_new_cstr("seqid");
caption1 = gt_str_new_cstr("foo");
caption2 = gt_str_new_cstr("bar");
testerr = gt_error_new();
r1.start = 10UL;
r1.end = 50UL;
r2.start = 40UL;
r2.end = 50UL;
gn1 = gt_feature_node_new(seqid, gt_ft_gene, r1.start, r1.end,
GT_STRAND_FORWARD);
gn2 = gt_feature_node_new(seqid, gt_ft_exon, r2.start, r2.end,
GT_STRAND_FORWARD);
e1 = gt_element_new((GtFeatureNode*) gn1);
e2 = gt_element_new((GtFeatureNode*) gn2);
b = gt_block_new();
/* test gt_block_insert_elements */
gt_ensure((0UL == gt_block_get_size(b)));
gt_block_insert_element(b, (GtFeatureNode*) gn1);
gt_ensure((1UL == gt_block_get_size(b)));
gt_block_insert_element(b, (GtFeatureNode*) gn2);
gt_ensure((2UL == gt_block_get_size(b)));
/* test gt_block_set_range & gt_block_get_range */
r_temp = gt_range_join(&r1, &r2);
gt_block_set_range(b, r_temp);
b_range = gt_block_get_range(b);
gt_ensure((0 == gt_range_compare(&b_range, &r_temp)));
gt_ensure((1 == gt_range_compare(&r2, &r_temp)));
/* tests gt_block_set_caption & gt_block_get_caption */
gt_block_set_caption(b, caption1);
gt_ensure((0 == gt_str_cmp(gt_block_get_caption(b), caption1)));
gt_ensure((0 != gt_str_cmp(gt_block_get_caption(b), caption2)));
/* tests gt_block_set_strand & gt_block_get_range */
s = gt_block_get_strand(b);
gt_ensure((GT_STRAND_UNKNOWN == s));
gt_block_set_strand(b, GT_STRAND_FORWARD);
s = gt_block_get_strand(b);
gt_ensure((GT_STRAND_FORWARD == s));
/* test gt_block_get_max_height() */
sty = gt_style_new(err);
gt_ensure(gt_block_get_max_height(b, &height, sty, err) == 0);
gt_ensure(!gt_error_is_set(testerr));
gt_ensure(height == BAR_HEIGHT_DEFAULT);
gt_style_set_num(sty, "exon", "bar_height", 42);
gt_ensure(gt_block_get_max_height(b, &height, sty, err) == 0);
gt_ensure(!gt_error_is_set(testerr));
gt_ensure(height == 42);
gt_style_set_num(sty, "gene", "bar_height", 23);
gt_ensure(gt_block_get_max_height(b, &height, sty, err) == 0);
gt_ensure(!gt_error_is_set(testerr));
gt_ensure(height == 42);
gt_style_unset(sty, "exon", "bar_height");
gt_ensure(gt_block_get_max_height(b, &height, sty, err) == 0);
gt_ensure(!gt_error_is_set(testerr));
gt_ensure(height == 23);
gt_str_delete(caption2);
gt_str_delete(seqid);
gt_element_delete(e1);
gt_element_delete(e2);
gt_block_delete(b);
gt_style_delete(sty);
gt_error_delete(testerr);
gt_genome_node_delete(gn1);
gt_genome_node_delete(gn2);
return had_err;
}
bool gth_sas_are_equal(const GthSA *saA, const GthSA *saB)
{
Exoninfo *exoninfoA, *exoninfoB;
Introninfo *introninfoA, *introninfoB;
GtUword i;
/* compare element 0 */
if (gth_sa_alphatype(saA) != gth_sa_alphatype(saB))
return false;
/* compare element 1 */
if (gth_backtrace_path_length(saA->backtrace_path) !=
gth_backtrace_path_length(saB->backtrace_path)) {
return false;
}
for (i = 0; i < gth_backtrace_path_length(saA->backtrace_path); i++) {
if (((Editoperation*) gth_backtrace_path_get(saA->backtrace_path))[i] !=
((Editoperation*) gth_backtrace_path_get(saB->backtrace_path))[i]) {
return false;
}
}
/* element 2 has been removed (indelcount) */
/* compare element 3 */
if (gth_sa_gen_dp_length(saA) != gth_sa_gen_dp_length(saB))
return false;
/* compare element 4 */
if (saA->gen_total_length != saB->gen_total_length)
return false;
/* compare element 5 */
if (saA->gen_offset != saB->gen_offset)
return false;
/* compare element 6 */
if (gth_sa_ref_total_length(saA) != gth_sa_ref_total_length(saB))
return false;
/* compare element 7 */
if (gth_sa_gen_dp_start(saA) != gth_sa_gen_dp_start(saB))
return false;
/* element 8 has been removed (gen_dp_end) */
/* compare element 9 */
if (saA->gen_file_num != saB->gen_file_num)
return false;
/* compare element 10 */
if (saA->gen_seq_num != saB->gen_seq_num)
return false;
/* compare element 11 */
if (saA->ref_file_num != saB->ref_file_num)
return false;
/* compare element 12 */
if (saA->ref_seq_num != saB->ref_seq_num)
return false;
/* compare element 13 */
if (gt_str_cmp(saA->gen_id, saB->gen_id))
return false;
/* compare element 14 */
if (gt_str_cmp(saA->ref_id, saB->ref_id))
return false;
/* compare element 15 */
if (saA->gen_strand_forward != saB->gen_strand_forward)
return false;
/* compare element 16 */
if (saA->ref_strand_forward != saB->ref_strand_forward)
return false;
/* compare element 17 */
if (gth_sa_genomiccutoff_start(saA) != gth_sa_genomiccutoff_start(saB))
return false;
if (gth_sa_referencecutoff_start(saA) != gth_sa_referencecutoff_start(saB))
return false;
if (gth_sa_eopcutoff_start(saA) != gth_sa_eopcutoff_start(saB))
return false;
if (gth_sa_genomiccutoff_end(saA) != gth_sa_genomiccutoff_end(saB))
return false;
if (gth_sa_referencecutoff_end(saA) != gth_sa_referencecutoff_end(saB))
return false;
if (gth_sa_eopcutoff_end(saA) != gth_sa_eopcutoff_end(saB))
return false;
/* compare element 18 */
if (gt_array_size(saA->exons) != gt_array_size(saB->exons))
return false;
for (i = 0; i < gt_array_size(saA->exons); i++) {
exoninfoA = (Exoninfo*) gt_array_get(saA->exons, i);
exoninfoB = (Exoninfo*) gt_array_get(saB->exons, i);
if (exoninfoA->leftgenomicexonborder != exoninfoB->leftgenomicexonborder)
return false;
if (exoninfoA->rightgenomicexonborder != exoninfoB->rightgenomicexonborder)
return false;
if (exoninfoA->leftreferenceexonborder !=
exoninfoB->leftreferenceexonborder) {
return false;
}
if (exoninfoA->rightreferenceexonborder !=
exoninfoB->rightreferenceexonborder) {
return false;
}
if (!gt_double_equals_double(exoninfoA->exonscore, exoninfoB->exonscore)) {
return false;
}
}
/* compare element 19 */
if (gt_array_size(saA->introns) != gt_array_size(saB->introns))
return false;
for (i = 0; i < gt_array_size(saA->introns); i++) {
introninfoA = (Introninfo*) gt_array_get(saA->introns, i);
introninfoB = (Introninfo*) gt_array_get(saB->introns, i);
if (!gt_double_equals_double(introninfoA->donorsiteprobability,
introninfoB->donorsiteprobability)) {
return false;
}
if (!gt_double_equals_double(introninfoA->acceptorsiteprobability,
introninfoB->acceptorsiteprobability)) {
return false;
}
if (!gt_double_equals_double(introninfoA->donorsitescore,
introninfoB->donorsitescore)) {
return false;
}
if (!gt_double_equals_double(introninfoA->acceptorsitescore,
introninfoB->acceptorsitescore)) {
return false;
}
}
/* compare element 20 */
if (saA->polyAtailpos.start != saB->polyAtailpos.start)
return false;
if (saA->polyAtailpos.end != saB->polyAtailpos.end)
return false;
/* compare element 21 */
if (saA->alignmentscore != saB->alignmentscore)
return false;
/* compare element 22 */
if (saA->coverage != saB->coverage)
return false;
/* compare element 23 */
if (saA->genomic_cov_is_highest != saB->genomic_cov_is_highest)
return false;
/* compare element 24 */
if (saA->cumlen_scored_exons != saB->cumlen_scored_exons)
return false;
return true;
}
static int update_seq_col_if_necessary(GtRegionMapping *rm, GtStr *seqid,
GtError *err)
{
int had_err = 0;
gt_error_check(err);
gt_assert(rm && seqid);
/* for mappings, we need to load the changed sequence, if needed... */
if (rm->mapping) {
if (!rm->sequence_file || (gt_str_cmp(rm->sequence_name, seqid))) {
gt_str_delete(rm->sequence_file);
/* ignore MD5 hashes when using region mappings */
if (gt_md5_seqid_has_prefix(gt_str_get(seqid))) {
rm->sequence_file = region_mapping_map(rm,
gt_str_get(seqid)
+GT_MD5_SEQID_TOTAL_LEN,
err);
} else
rm->sequence_file = region_mapping_map(rm, gt_str_get(seqid), err);
if (!rm->sequence_file)
had_err = -1;
else {
/* load new seqcol */
if (!rm->sequence_filenames)
rm->sequence_filenames = gt_str_array_new();
else
gt_str_array_reset(rm->sequence_filenames);
gt_str_array_add(rm->sequence_filenames, rm->sequence_file);
if (!rm->sequence_name)
rm->sequence_name = gt_str_new();
else
gt_str_reset(rm->sequence_name);
gt_str_append_str(rm->sequence_name, seqid);
gt_seq_col_delete(rm->seq_col);
rm->seq_col = gt_bioseq_col_new(rm->sequence_filenames, err);
if (!rm->seq_col)
had_err = -1;
}
}
} else {
/* ...otherwise, just make sure the seqcol is loaded */
if (!rm->seq_col) {
if (rm->encseq) {
if (!(rm->seq_col = gt_encseq_col_new(rm->encseq, err)))
had_err = -1;
} else {
gt_assert(rm->sequence_filenames);
if (!(rm->seq_col = gt_bioseq_col_new(rm->sequence_filenames, err)))
had_err = -1;
}
}
if (!had_err && rm->usedesc) {
if (rm->seqid2seqnum_mapping)
gt_seqid2seqnum_mapping_delete(rm->seqid2seqnum_mapping);
rm->seqid2seqnum_mapping =
gt_seqid2seqnum_mapping_new_seqcol(rm->seq_col, err);
if (!rm->seqid2seqnum_mapping) {
had_err = -1;
}
}
}
return had_err;
}
static int construct_genes(GT_UNUSED void *key, void *value, void *data,
GtError *err)
{
GtHashmap *transcript_id_hash = (GtHashmap*) value;
ConstructionInfo *cinfo = (ConstructionInfo*) data;
GtQueue *genome_nodes = cinfo->genome_nodes;
const char *gname;
GtArray *mRNAs = gt_array_new(sizeof (GtGenomeNode*));
GtGenomeNode *gene_node, *gn;
GtStrand gene_strand;
GtRange gene_range;
GtStr *gene_seqid;
GtUword i;
int had_err = 0;
gt_error_check(err);
gt_assert(key && value && data);
cinfo->mRNAs = mRNAs;
had_err = gt_hashmap_foreach(transcript_id_hash, construct_mRNAs, cinfo, err);
if (!had_err) {
gt_assert(gt_array_size(mRNAs)); /* at least one mRNA constructed */
/* determine the range and the strand of the gene */
gn = *(GtGenomeNode**) gt_array_get(mRNAs, 0);
gene_range = gt_genome_node_get_range(gn);
gene_strand = gt_feature_node_get_strand((GtFeatureNode*) gn);
gene_seqid = gt_genome_node_get_seqid(gn);
for (i = 1; i < gt_array_size(mRNAs); i++) {
GtRange range;
gn = *(GtGenomeNode**) gt_array_get(mRNAs, i);
range = gt_genome_node_get_range(gn);
gene_range = gt_range_join(&gene_range, &range);
gene_strand = gt_strand_join(gene_strand,
gt_feature_node_get_strand((GtFeatureNode*) gn));
gt_assert(gt_str_cmp(gene_seqid, gt_genome_node_get_seqid(gn)) == 0);
}
gene_node = gt_feature_node_new(gene_seqid, gt_ft_gene, gene_range.start,
gene_range.end, gene_strand);
if ((gname = gt_hashmap_get(cinfo->gene_id_to_name_mapping,
(const char*) key))) {
gt_feature_node_add_attribute((GtFeatureNode*) gene_node, GT_GFF_NAME,
gname);
}
/* register children */
for (i = 0; i < gt_array_size(mRNAs); i++) {
gn = *(GtGenomeNode**) gt_array_get(mRNAs, i);
gt_feature_node_add_child((GtFeatureNode*) gene_node,
(GtFeatureNode*) gn);
}
/* store the gene */
gt_queue_add(genome_nodes, gene_node);
/* free */
gt_array_delete(mRNAs);
}
return had_err;
}
static int construct_mRNAs(GT_UNUSED void *key, void *value, void *data,
GtError *err)
{
ConstructionInfo *cinfo = (ConstructionInfo*) data;
GtArray *gt_genome_node_array = (GtArray*) value,
*mRNAs = (GtArray*) cinfo->mRNAs;
GtGenomeNode *mRNA_node, *first_node, *gn;
const char *tname;
GtStrand mRNA_strand;
GtRange mRNA_range;
GtStr *mRNA_seqid;
GtUword i;
int had_err = 0;
gt_error_check(err);
gt_assert(key && value && data);
/* at least one node in array */
gt_assert(gt_array_size(gt_genome_node_array));
/* determine the range and the strand of the mRNA */
first_node = *(GtGenomeNode**) gt_array_get(gt_genome_node_array, 0);
mRNA_range = gt_genome_node_get_range(first_node);
mRNA_strand = gt_feature_node_get_strand((GtFeatureNode*) first_node);
mRNA_seqid = gt_genome_node_get_seqid(first_node);
for (i = 1; i < gt_array_size(gt_genome_node_array); i++) {
GtRange range;
gn = *(GtGenomeNode**) gt_array_get(gt_genome_node_array, i);
range = gt_genome_node_get_range(gn);
mRNA_range = gt_range_join(&mRNA_range, &range);
/* XXX: an error check is necessary here, otherwise gt_strand_join() can
cause a failed assertion */
mRNA_strand = gt_strand_join(mRNA_strand,
gt_feature_node_get_strand((GtFeatureNode*) gn));
if (gt_str_cmp(mRNA_seqid, gt_genome_node_get_seqid(gn))) {
gt_error_set(err, "The features on lines %u and %u refer to different "
"genomic sequences (``seqname''), although they have the same "
"gene IDs (``gene_id'') which must be globally unique",
gt_genome_node_get_line_number(first_node),
gt_genome_node_get_line_number(gn));
had_err = -1;
break;
}
}
if (!had_err) {
mRNA_node = gt_feature_node_new(mRNA_seqid, gt_ft_mRNA, mRNA_range.start,
mRNA_range.end, mRNA_strand);
if ((tname = gt_hashmap_get(cinfo->transcript_id_to_name_mapping,
(const char*) key))) {
gt_feature_node_add_attribute((GtFeatureNode*) mRNA_node, GT_GFF_NAME,
tname);
}
/* register children */
for (i = 0; i < gt_array_size(gt_genome_node_array); i++) {
gn = *(GtGenomeNode**) gt_array_get(gt_genome_node_array, i);
gt_feature_node_add_child((GtFeatureNode*) mRNA_node,
(GtFeatureNode*) gn);
}
/* store the mRNA */
gt_array_add(mRNAs, mRNA_node);
}
return had_err;
}
static int construct_mRNAs(GT_UNUSED void *key, void *value, void *data,
GtError *err)
{
ConstructionInfo *cinfo = (ConstructionInfo*) data;
GtArray *gt_genome_node_array = (GtArray*) value,
*mRNAs = (GtArray*) cinfo->mRNAs;
GtGenomeNode *mRNA_node, *first_node, *gn;
const char *tname;
GtStrand mRNA_strand;
GtRange mRNA_range;
GtStr *mRNA_seqid;
GtUword i;
int had_err = 0;
gt_error_check(err);
gt_assert(key && value && data);
/* at least one node in array */
gt_assert(gt_array_size(gt_genome_node_array));
/* determine the range and the strand of the mRNA */
first_node = *(GtGenomeNode**) gt_array_get(gt_genome_node_array, 0);
mRNA_range = gt_genome_node_get_range(first_node);
mRNA_strand = gt_feature_node_get_strand((GtFeatureNode*) first_node);
mRNA_seqid = gt_genome_node_get_seqid(first_node);
/* TODO: support discontinuous start/stop codons */
for (i = 0; !had_err && i < gt_array_size(gt_genome_node_array); i++) {
gn = *(GtGenomeNode**) gt_array_get(gt_genome_node_array, i);
if (gt_feature_node_get_attribute((GtFeatureNode*) gn,
GTF_PARSER_STOP_CODON_FLAG)) {
GtUword j;
GtRange stop_codon_rng = gt_genome_node_get_range(gn);
bool found_cds = false;
for (j = 0; !had_err && j < gt_array_size(gt_genome_node_array); j++) {
GtGenomeNode* gn2;
GtRange this_rng;
const char *this_type;
gn2 = *(GtGenomeNode**) gt_array_get(gt_genome_node_array, j);
if (gn == gn2) continue;
this_rng = gt_genome_node_get_range(gn2);
this_type = gt_feature_node_get_type((GtFeatureNode*) gn2);
if (this_type == gt_symbol(gt_ft_CDS)) {
if (gt_range_contains(&this_rng, &stop_codon_rng)) {
if (cinfo->tidy) {
gt_warning("stop codon on line %u in file %s is contained in "
"CDS in line %u",
gt_genome_node_get_line_number(gn),
gt_genome_node_get_filename(gn),
gt_genome_node_get_line_number(gn2));
found_cds = true;
} else {
gt_error_set(err, "stop codon on line %u in file %s is "
"contained in CDS in line %u",
gt_genome_node_get_line_number(gn),
gt_genome_node_get_filename(gn),
gt_genome_node_get_line_number(gn2));
had_err = -1;
}
break;
}
if (this_rng.end + 1 == stop_codon_rng.start) {
this_rng.end = stop_codon_rng.end;
gt_genome_node_set_range(gn2, &this_rng);
found_cds = true;
break;
}
if (this_rng.start == stop_codon_rng.end + 1) {
this_rng.start = stop_codon_rng.start;
gt_genome_node_set_range(gn2, &this_rng);
found_cds = true;
break;
}
}
}
if (!found_cds) {
if (!had_err) {
if (cinfo->tidy) {
gt_warning("found stop codon on line %u in file %s with no "
"flanking CDS, ignoring it",
gt_genome_node_get_line_number(gn),
gt_genome_node_get_filename(gn));
} else {
gt_error_set(err, "found stop codon on line %u in file %s with no "
"flanking CDS",
gt_genome_node_get_line_number(gn),
gt_genome_node_get_filename(gn));
had_err = -1;
break;
}
}
} else {
gt_array_rem(gt_genome_node_array, i);
gt_genome_node_delete(gn);
}
}
}
for (i = 1; !had_err && i < gt_array_size(gt_genome_node_array); i++) {
GtRange range;
GtStrand strand;
gn = *(GtGenomeNode**) gt_array_get(gt_genome_node_array, i);
range = gt_genome_node_get_range(gn);
mRNA_range = gt_range_join(&mRNA_range, &range);
strand = gt_feature_node_get_strand((GtFeatureNode*) gn);
if (strand != mRNA_strand) {
gt_error_set(err, "feature %s on line %u has strand %c, but the "
"parent transcript has strand %c",
(const char*) key,
gt_genome_node_get_line_number(gn),
GT_STRAND_CHARS[strand],
GT_STRAND_CHARS[mRNA_strand]);
had_err = -1;
break;
} else {
mRNA_strand = gt_strand_join(mRNA_strand, strand);
}
if (!had_err && gt_str_cmp(mRNA_seqid, gt_genome_node_get_seqid(gn))) {
gt_error_set(err, "The features on lines %u and %u refer to different "
"genomic sequences (``seqname''), although they have the same "
"gene IDs (``gene_id'') which must be globally unique",
gt_genome_node_get_line_number(first_node),
gt_genome_node_get_line_number(gn));
had_err = -1;
break;
}
}
if (!had_err) {
mRNA_node = gt_feature_node_new(mRNA_seqid, gt_ft_mRNA, mRNA_range.start,
mRNA_range.end, mRNA_strand);
gt_feature_node_add_attribute(((GtFeatureNode*) mRNA_node), "ID", key);
gt_feature_node_add_attribute(((GtFeatureNode*) mRNA_node), "transcript_id",
key);
if ((tname = gt_hashmap_get(cinfo->transcript_id_to_name_mapping,
(const char*) key)) && strlen(tname) > 0) {
gt_feature_node_add_attribute((GtFeatureNode*) mRNA_node, GT_GFF_NAME,
tname);
}
/* register children */
for (i = 0; i < gt_array_size(gt_genome_node_array); i++) {
gn = *(GtGenomeNode**) gt_array_get(gt_genome_node_array, i);
gt_feature_node_add_child((GtFeatureNode*) mRNA_node,
(GtFeatureNode*) gt_genome_node_ref(gn));
}
/* store the mRNA */
gt_array_add(mRNAs, mRNA_node);
}
return had_err;
}
