int main(int argc, char *argv[])
{
int m_sock,i,j, autoexp_gain;
char autoe_filename[500];
char autoe_dirname[500];
char command_buffer[255]; // buffer for commands to be executed
char image_dir[255];
sprintf(image_dir, "%s/polonator/G.007/acquisition", getenv("HOME"));
sprintf(command_buffer, "mkdir -p %s", image_dir);
system(command_buffer);
if(argc < 2)
{
fprintf(stdout, "%s must be called w/ one of the following arguments:\n", argv[0]);
fprintf(stdout, "\treset\t\t -- restart software on controller and home all axes\n");
fprintf(stdout, "\tunlock\t\t -- 'unlock' stage for manual movement\n");
fprintf(stdout, "\tlock\t\t -- 'lock' stage for servo movement\n");
fprintf(stdout, "\tlive\t\t -- display live darkfield image (%s live <integration in seconds> <EM gain>)\n", argv[0]);
fprintf(stdout, "\tsnap\t\t -- snap image and save to disk (%s snap <color[fam, cy5, cy3, txred, spare, or none]> <integration in seconds> <EM gain>)\n", argv[0]);
fprintf(stdout, "\tcolorsnap\t -- snap images in all 4 channels and save to disk (%s colorsnap <integration in seconds> <fam gain> <cy5 gain> <cy3 gain> <txred gain>)\n", argv[0]);
fprintf(stdout, "\tstatus\t\t -- get motion controller status and last errors, if any\n");
fprintf(stdout, "\tcomplete-scan\t -- if scanning was interrupted, and reset the controller software gracefully to its starting state\n");
fprintf(stdout, "\tdarkfield-off\t -- turn the darkfield ring illuminator off\n");
fprintf(stdout, "\tdarkfield-on\t -- turn the darkfield ring illuminator on\n");
fprintf(stdout, "\thometheta\t -- 'lock' theta (filter cube) axis and re-home\n");
fprintf(stdout, "\tunlocktheta\t -- 'unlock' theta (filter cube) axis\n");
fprintf(stdout, "\tpower-on\t -- turn camera and fluorescence light source on\n");
fprintf(stdout, "\tpower-off\t -- turn camera and fluorescence light source off\n");
fprintf(stdout, "\tgotostagealignpos\t -- goto position used for stage alignment (%s gotostagealignpos <flowcell> <lane>)\n", argv[0]);
fprintf(stdout, "\tgetfocus\t -- get current 'focus' value from Z axis (this is the offset applied to focus error signal)\n");
fprintf(stdout, "\tsetfocus\t -- set focus value for Z axis; note this DOES NOT write to non-volatile memory (%s setfocus <focus_value>)\n", argv[0]);
fprintf(stdout, "\twritefocus\t -- write current focus value to non-volatile memory; should be used after correct value is found with setfocus\n");
exit(1);
}
/* Reset the controller software and re-home the stage */
if(strcmp(argv[1], "reset") == 0) {
maestro_open(&m_sock);
maestro_reset(m_sock);
}
else if(strcmp(argv[1], "unlock") == 0) {
maestro_open(&m_sock);
maestro_unlock(m_sock);
}
else if(strcmp(argv[1], "lock") == 0) {
maestro_open(&m_sock);
maestro_lock(m_sock);
}
else if(strcmp(argv[1], "getfocus") == 0) {
maestro_open(&m_sock);
return maestro_getfocus(m_sock);
}
else if(strcmp(argv[1], "setfocus") == 0) {
maestro_open(&m_sock);
maestro_setfocus(m_sock, atoi(argv[2]));
}
else if(strcmp(argv[1], "writefocus") == 0) {
maestro_open(&m_sock);
maestro_writefocus(m_sock);
}
/* Display a live darkfield image */
else if((strcmp(argv[1], "live") == 0) && ((argc == 4)||(argc == 5))) {
maestro_open(&m_sock);
maestro_darkfield_on(m_sock);
maestro_setcolor(m_sock, "none");
if(argc == 4)
{
camera_live(argc, argv);
}
else {
camera_live(argc, argv);
}
maestro_darkfield_off(m_sock);
}
else if((strcmp(argv[1], "live_new") == 0) && ((argc == 5)||(argc == 6)))
{
maestro_open(&m_sock);
maestro_setcolor(m_sock, argv[4]);
if(strcmp(argv[4],"none") == 0)
{
maestro_darkfield_on(m_sock);
maestro_shutterclose(m_sock);
}
else
{
maestro_darkfield_off(m_sock);
maestro_shutteropen(m_sock);
}
fprintf(stdout, "Live view starting");
if(argc==5) {
fprintf(stdout, "Live view argc == 5");
camera_live(argc, argv);
}
else {
fprintf(stdout, "Live view argc != 5");
camera_live(argc, argv);
}
fprintf(stdout, "Live view done");
if(strcmp(argv[4],"none")==0) {
maestro_darkfield_off(m_sock);
}
else {
maestro_shutterclose(m_sock);
}
}
else if (strcmp(argv[1],"shutter_close")==0)
{
maestro_open(&m_sock);
maestro_shutterclose(m_sock);
}
/* Acquire an image at the current stage position */
else if((strcmp(argv[1], "snap") == 0) && (argc == 5)) {
sprintf(command_buffer, "%s/snap-image.raw", image_dir);
snap(atof(argv[3]), atof(argv[4]), argv[2], command_buffer);
}
else if((strcmp(argv[1], "snap1") == 0) && (argc == 6))
{
sprintf(command_buffer, "mkdir -p %s/autoexp_FL_images/cy3", image_dir);
system(command_buffer);
sprintf(command_buffer, "mkdir -p %s/autoexp_FL_images/fam", image_dir);
system(command_buffer);
sprintf(command_buffer, "mkdir -p %s/autoexp_FL_images/cy5", image_dir);
system(command_buffer);
sprintf(command_buffer, "mkdir -p %s/autoexp_FL_images/txred", image_dir);
system(command_buffer);
for(j = 0; j < atoi(argv[5]); j++)
{
maestro_open(&m_sock);
maestro_gotostagealign_position(m_sock, 0, j);
for(i = 0; i < 15; i++)
{
attempt_counter = 0;
autoexp_gain = atoi(argv[4])+i*10;
sprintf(autoe_filename, " ... start acquring FL image in lane %d, for %s with autoexposure gain of %d ... ",j, argv[2],autoexp_gain);
p_log_simple(autoe_filename);
sprintf(autoe_filename, "%s/autoexp_FL_images/%s/%d_image_%d.raw", image_dir,argv[2],j,autoexp_gain);
p_log_simple(autoe_filename);
wait_counter = 0;
snap(atof(argv[3]), autoexp_gain, argv[2], autoe_filename);
sprintf(autoe_filename, " ... acquired FL image in %d ms ...\n", wait_counter);
p_log_simple(autoe_filename);
while((attempt_counter < 4) && (attempt_counter > 0))
{
sprintf(autoe_filename, "... ACQUIRING FAILED !!! Re-acquring FL image in lane %d, for %s with autoexposure gain of %d ...\n",atoi(argv[5]), argv[2],atoi(argv[4]));
p_log_errorno(autoe_filename);
snap(atof(argv[3]), atof(argv[4]), argv[2], autoe_filename);
}
} // end for i
} // end for j
} // end else if
/* Acquire 4 fluorescence images */
else if((strcmp(argv[1], "colorsnap") == 0) && (argc == 7)) {
sprintf(command_buffer, "%s/colorsnap-fam.raw", image_dir);
snap(atof(argv[2]), atof(argv[3]), "fam", command_buffer);
sprintf(command_buffer, "%s/colorsnap-cy5.raw", image_dir);
snap(atof(argv[2]), atof(argv[4]), "cy5", command_buffer);
sprintf(command_buffer, "%s/colorsnap-cy3.raw", image_dir);
snap(atof(argv[2]), atof(argv[5]), "cy3", command_buffer);
sprintf(command_buffer, "%s/colorsnap-txr.raw", image_dir);
snap(atof(argv[2]), atof(argv[6]), "txred", command_buffer);
}
else if(strcmp(argv[1], "complete-scan") == 0) {
maestro_open(&m_sock);
maestro_stop(m_sock);
}
else if(strcmp(argv[1], "darkfield-off") == 0) {
maestro_open(&m_sock);
maestro_darkfield_off(m_sock);
}
else if(strcmp(argv[1], "darkfield-on") == 0) {
maestro_open(&m_sock);
maestro_darkfield_on(m_sock);
}
else if(strcmp(argv[1], "hometheta") == 0) {
maestro_open(&m_sock);
maestro_hometheta(m_sock);
}
else if(strcmp(argv[1], "unlocktheta") == 0) {
maestro_open(&m_sock);
maestro_unlocktheta(m_sock);
}
else if(strcmp(argv[1], "status") == 0) {
maestro_open(&m_sock);
maestro_getstatus(m_sock);
}
else if(strcmp(argv[1], "power-on") == 0) {
network_iboot_on(&m_sock);
}
else if(strcmp(argv[1], "power-off") == 0) {
network_iboot_off(&m_sock);
}
else if(strcmp(argv[1], "gotostagealignpos") == 0) {
maestro_open(&m_sock);
maestro_gotostagealign_position(m_sock, atoi(argv[2]), atoi(argv[3]));
}
else
{
main(1, argv);
}
} // end function
void stagealign(int fcnum, int lane_num, int initialize)
{
short unsigned int *testimage;
short unsigned int *baseimage;
FILE *baseimgfp;
FILE *offsetfp;
/* used to dump score matrix to file when debugging */
FILE *score_matrixfp;
/* Maestro declarations */
int m_sock; /* socket for the Maestro controller */
char config_value[255];
char logfilename[255];
char offsetfilename[255];
char stagealign_baseimgfilename[255];
char acqcfgpath[255];
/* Used to send commands and receive responses from Maestro */
char command[255];
char response[255];
int response_length;
/* Image acquisition settings, populated from config file */
int stagealign_integration_inmsec;
int stagealign_gain;
int stagealign_well;
int stagealign_wells_per_fc;
/* Values used for conversion between pixels and stage units */
int stagealign_optical_mag;
int ccd_pixel_size;
double pixelsize_at_stage; /* size of a pixel at the stage in microns */
/* Hold offsets found by alignment in pixels and stageunits */
int pixel_offset_x, pixel_offset_y;
double stageunit_offset_x, stageunit_offset_y;
int lane_index;
/* Holds previous offsets from controller in case the alignment doesn't work */
int curr_offset_x, curr_offset_y;
/* Holds encoder resolutions from controller, used for calculating distance of move */
double encoder_res_X, encoder_res_Y;
/* 'score' of best alignment */
int score;
/* Largest pixel offset allowable _after_ the adjustment has been made */
/* if there is still a shift larger than this, conclude something is wrong */
/* and go back to the previous offset */
int successful_move_threshold = 150; /*RCT was = 12*/
int i;
/* in debugging mode, dump internal data to files */
#ifdef DEBUG_STAGEALIGN
FILE *imgfp;
sprintf(command, "%s/stagealign-image%d_%d.raw", output_directory, fcnum, lane_num);
imgfp = fopen(command, "w");
sprintf(command, "%s/stagealign-scorematrix%d_%d", output_directory, fcnum, lane_num);
score_matrixfp = fopen(command, "w");
#endif
p_log_simple("awesome2\n");
/* Open config file */
strcpy(acqcfgpath, getenv("POLONATOR_PATH"));
strcat(acqcfgpath, "/config_files/polonator-acq.cfg");
config_open(acqcfgpath);
p_log_simple("awesome1\n");
/* Initialize variables */
if(!config_getvalue("stagealign_logfilename", config_value)){
fprintf(stderr, "ERROR:\tPolonator-stagealign: config_getval(key logfilename) returned no value\n");
exit(0);
}
p_log_simple("awesome0\n");
strcpy(logfilename, log_dir);
strcat(logfilename, "/");
strcat(logfilename, config_value);
sprintf(command, "%d", fcnum);
strcat(logfilename, command);
strcat(logfilename, ".log");
p_log_simple(logfilename);
start_logger(logfilename, 1);
strcpy(offsetfilename, log_dir);
strcat(offsetfilename, "/");
strcat(offsetfilename, config_value);
strcat(offsetfilename, command);
strcat(offsetfilename, ".offsetlog");
fprintf(stdout, offsetfilename);
fflush(stdout);
/*
if this is being run in 'initialize mode' -- the first scan of a run --
overwrite the offset logfile
*/
p_log_simple("awesome66\n");
if(initialize){
offsetfp = fopen(offsetfilename, "w");
}
else{
offsetfp = fopen(offsetfilename, "a");
}
p_log_simple("awesome00\n");
if(!config_getvalue("stagealign_baseimgfilename", config_value)){
p_log("ERROR:\tPolonator-stagealign: config_getval(key stagealign_baseimgfilename) returned no value");
exit(0);
}
sprintf(stagealign_baseimgfilename, "%s/%s%s_%d.raw", output_directory, config_value, command, lane_num);
p_log_simple("awesome01\n");
if(!config_getvalue("stagealign_integration_inmsec", config_value)){
p_log("ERROR:\tPolonator-stagealign: config_getval(key stagealign_integration_inmsec) returned no value");
exit(0);
}
stagealign_integration_inmsec = atoi(config_value);
p_log_simple("awesome02\n");
if(!config_getvalue("stagealign_gain", config_value)){
p_log("ERROR:\tPolonator-stagealign: config_getval(key stagealign_gain) returned no value");
exit(0);
}
stagealign_gain = atoi(config_value);
p_log_simple("awesome03\n");
if(!config_getvalue("stagealign_optical_mag", config_value)){
p_log("ERROR:\tPolonator-stagealign: config_getval(key stagealign_optical_mag) returned no value");
exit(0);
}
stagealign_optical_mag = atoi(config_value);
p_log_simple("awesome04\n");
if(!config_getvalue("stagealign_ccd_pixel_size", config_value)){
p_log("ERROR:\tPolonator-stagealign: config_getval(key stagealign_ccd_pixel_size) returned no value");
exit(0);
}
ccd_pixel_size = atoi(config_value);
p_log_simple("awesome05\n");
if(!config_getvalue("stagealign_wells_per_fc", config_value)){
p_log("ERROR:\tPolonator-stagealign: config_getval(key stagealign_wells_per_fc) returned no value");
exit(0);
}
stagealign_wells_per_fc = atoi(config_value);
config_close();
p_log_simple("awesome06\n");
stagealign_well = lane_num;
lane_index = (fcnum * stagealign_wells_per_fc) + stagealign_well;
baseimage = (short unsigned int*)malloc(1000000 * sizeof(short unsigned int));
/*--------------------------------------------------------------------------
//
// MAESTRO SETUP
/*/
p_log_simple("STATUS:\tPolonator-stagealign: Opening connection to Maestro...");
maestro_open(&m_sock);
/*
//--------------------------------------------------------------------------
*/
/*--------------------------------------------------------------------------
//
// CAMERA SETUP
/*/
p_log_simple("STATUS:\tPolonator-stagealign: Opening camera handle...");
py_cameraInit(0); /* use non-TDI config file */
py_set_gain(stagealign_gain);
py_setupSnap(); /* setup capture software to wait for images from camera */
/*
//--------------------------------------------------------------------------
*/
/*p_log("STATUS:\tPolonator-stagealign: Darkfield illuminator on..."); rolony*/
/*maestro_darkfield_on(m_sock);
p_log("STATUS:\tPolonator-stagealign: Select darkfield filter block..."); rolony*/
maestro_setcolor(m_sock, "cy5");
/* IF INITIALIZING, RESET OFFSETS */
if(initialize){
p_log_simple("INITIALIZING STAGEALIGN");
sprintf(command, "PolonatorScan.OFFSET_X[%d]=0\n\r", lane_index);
p_log_simple(command);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
p_log_simple(response);
sprintf(command, "PolonatorScan.OFFSET_Y[%d]=0\n\r", lane_index);
p_log_simple(command);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
p_log_simple(response);
}
/* GET OFFSETS IN CASE ALIGNMENT FAILS */
else{
p_log_simple("Storing current offsets...");
sprintf(command, "PolonatorScan.OFFSET_X[%d]\n\r", lane_index);
p_log_simple(command);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
p_log_simple(response);
curr_offset_x = atoi(response);
sprintf(command, "PolonatorScan.OFFSET_Y[%d]\n\r", lane_index);
p_log_simple(command);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
p_log_simple(response);
curr_offset_y = atoi(response);
}
/* MOVE STAGE TO ORIGIN */
maestro_gotostagealign_position(m_sock, fcnum, lane_num);
p_log_simple("awesome fool00\n");
/* ACQUIRE IMAGE */
p_log("STATUS:\tPolonator-stagealign: Acquire image...");
maestro_snap(m_sock, stagealign_integration_inmsec, 1); /*rolony*/
while(!py_snapReceived()){;}
testimage = py_getSnapImage();
/* IF INITIALIZING, RE-WRITE THE BASE IMAGE; THE OFFSET FOUND SHOULD BE ZERO */
p_log_simple(stagealign_baseimgfilename);
if(initialize){
baseimgfp = fopen(stagealign_baseimgfilename, "w");
fwrite(testimage, 1000000, sizeof(short unsigned int), baseimgfp);
fclose(baseimgfp);
}
p_log_simple("awesome fool01\n");
#ifdef DEBUG_STAGEALIGN
fwrite(testimage, 1000000, sizeof(short unsigned int), imgfp);
#endif
/* LOAD BASE IMAGE */
p_log("STATUS:\tPolonator-stagealign: Load base image and determine offset...");
baseimgfp = fopen(stagealign_baseimgfilename, "r");
fread(baseimage, 1000000, sizeof(short unsigned int), baseimgfp);
fclose(baseimgfp);
p_log("STATUS:\tPolonator-stagealign: Load base image and determine offset2...");
/* DETERMINE OFFSETS */
register_image(baseimage, testimage, &pixel_offset_x, &pixel_offset_y, &score, score_matrixfp);
sprintf(log_string, "STATUS:\tPolonator-stagealign: Found pixel offsets X:%d, Y:%d, score:%d", pixel_offset_x, pixel_offset_y, score);
p_log(log_string);
/* LOAD ENCODER RESOLUTIONS FOR CONVERSION BELOW; THESE ARE STORED ON */
/* THE CONTROLLER AS COUNTS PER MILLIMETER */
p_log("Retrieving encoder resolutions...");
sprintf(command, "PolonatorScan.cENCODER_X_RESOLUTION\n\r");
p_log(command);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
p_log(response);
encoder_res_X = atof(response);
sprintf(command, "PolonatorScan.cENCODER_Y_RESOLUTION\n\r");
p_log(command);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
p_log(response);
encoder_res_Y = atof(response);
/* CONVERT FROM PIXELS TO STAGE UNITS */
/* CALCULATE PIXEL SIZE IN MILLIMTERS AT THE STAGE BASED */
/* ON THE MAGNIFICATION AND THE CCD PIXEL SIZE */
pixelsize_at_stage = ((double)ccd_pixel_size / (double)stagealign_optical_mag) / 1000;
stageunit_offset_x = (double)pixel_offset_x * pixelsize_at_stage * encoder_res_X * -1;
stageunit_offset_y = (double)pixel_offset_y * pixelsize_at_stage * encoder_res_Y * -1;
/* SET NEW OFFSETS ON CONTROLLER USING VALUES */
/* CALCULATED ABOVE */
sprintf(command, "PolonatorScan.OFFSET_X[%d]\n\r", lane_index);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
fprintf(offsetfp, "%d\t%d\t", fcnum, stagealign_well);
fprintf(offsetfp, "%d\t", atoi(response));
sprintf(command, "PolonatorScan.OFFSET_Y[%d]\n\r", lane_index);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
fprintf(offsetfp, "%d\t", atoi(response));
fprintf(offsetfp, "%d\t%d\t%d\t%d\t", (int)stageunit_offset_x, (int)stageunit_offset_y, pixel_offset_x, pixel_offset_y);
/* ISSUE COMMANDS TO ADJUST STAGE COORDS */
p_log("STATUS:\tPolonator-stagealign: Set offset variables on Maestro...");
sprintf(command, "PolonatorScan.OFFSET_X[%d]=PolonatorScan.OFFSET_X[%d]+%d\n\r", lane_index, lane_index, (int)stageunit_offset_x);
p_log(command);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
p_log(response);
sprintf(command, "PolonatorScan.OFFSET_Y[%d]=PolonatorScan.OFFSET_Y[%d]+%d\n\r", lane_index, lane_index, (int)stageunit_offset_y);
p_log(command);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
p_log(response);
/* MOVE, THEN ACQUIRE ANOTHER IMAGE TO VERIFY OFFSET WORKED */
/* maestro_gotostagealign_position(m_sock, fcnum, lane_num);
maestro_snap(m_sock, stagealign_integration_inmsec, 0);
while(!py_snapReceived()){;}
testimage = py_getSnapImage();
*/
#ifdef DEBUG_STAGEALIGN
fwrite(testimage, 1000000, sizeof(short unsigned int), imgfp);
fclose(imgfp);
#endif
/* DETERMINE OFFSET TO CONFIRM */
/* p_log("STATUS:\tPolonator-stagealign: Re-compute alignment to verify move...");
register_image(baseimage, testimage, &pixel_offset_x, &pixel_offset_y, &score, score_matrixfp);
sprintf(log_string, "STATUS:\tPolonator-stagealign: Found pixel offsets X:%d, Y:%d, score:%d", pixel_offset_x, pixel_offset_y, score);
p_log(log_string);
fprintf(offsetfp, "%d\t%d", pixel_offset_x, pixel_offset_y);
*/
/* DID THE MOVE WORK? */
if(((abs(pixel_offset_x)>successful_move_threshold) || (abs(pixel_offset_y)>successful_move_threshold)) && (!initialize))
{
sprintf(log_string, "ERROR:\tPolonator-stagealign: one or more offsets are greater that the %d-pixel maximum; X:%d, Y:%d",
successful_move_threshold,
pixel_offset_x,
pixel_offset_y);
p_log(log_string);
fprintf(offsetfp, "*");
/* mark current line in offsetlog, since offsets found will not be the offsets stored on the controller */
sprintf(log_string, "Restoring previous offsets X:%d, Y:%d", curr_offset_x, curr_offset_y);
sprintf(command, "PolonatorScan.OFFSET_X[%d]=%d\n\r", lane_index, curr_offset_x);
p_log(command);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
p_log(response);
sprintf(command, "PolonatorScan.OFFSET_Y[%d]=%d\n\r", lane_index, curr_offset_y);
p_log(command);
send(m_sock, command, strlen(command), 0);
maestro_readresponse(m_sock, response, &response_length);
p_log(response);
}
/* EXIT */
#ifdef DEBUG_STAGEALIGN
fclose(score_matrixfp);
#endif
fprintf(offsetfp, "\n");
fclose(offsetfp);
/*maestro_darkfield_off(m_sock); rolony*/
py_cameraClose();
free(baseimage);
close_logger();
p_log_simple("awesome fool02\n");
}
void snap(float exposure, float gain, char *color, char *filename) {
int m_sock;
short unsigned int *image;
int imgmean;
int shutterflag;
FILE *outfile;
wait_counter = 0;
// open hardware and file
camera_init(); // use non-TDI config file
setGain(gain);
maestro_open(&m_sock);
outfile = fopen(filename, "w");
// configure hardware
maestro_setcolor(m_sock, color);
//maestro_darkfield_off(m_sock);
//setGain(gain);moved to line 174
// determine whether or not to use the shutter
if(!strcmp(color, "none"))
{
shutterflag = 0;
maestro_darkfield_on(m_sock);
}
else
{
shutterflag = 1;
maestro_darkfield_off(m_sock);
}
// setup the software to receive an image from the camera
setupSnap();
// snap the image
maestro_snap(m_sock, (int)(exposure * 1000.0), shutterflag);
// wait for image to be received by framegrabber
while(!snapReceived())
{
wait_counter++;
usleep(1000);
if(wait_counter > 20000) {
attempt_counter++;
network_iboot_off(&m_sock);
sleep(1);
network_iboot_on(&m_sock);
sleep(1);
wait_counter = 0;
}
}
// get pointer to image
image = getSnapImage();
// calculate mean for informational purposes, then write image to file
imgmean = imagemean(image);
fprintf(stdout, "Image mean: %d\n", imgmean);
fwrite(image, sizeof(short unsigned int), 1000000, outfile);
fprintf(stdout, "finish outputing image");
// close hardware and file
if(!strcmp(color, "none")) maestro_darkfield_off(m_sock);
fclose(outfile);
fprintf(stdout, "closing camera");
camera_close();
} // end function
