void QmitkTrackingWorker::run()
{
m_View->m_GlobalTracker = QmitkGibbsTrackingView::GibbsTrackingFilterType::New();
MITK_INFO << "Resampling mask images";
// setup resampler
typedef itk::ResampleImageFilter<QmitkGibbsTrackingView::MaskImgType, QmitkGibbsTrackingView::MaskImgType, float> ResamplerType;
ResamplerType::Pointer resampler = ResamplerType::New();
resampler->SetOutputSpacing( m_View->m_ItkQBallImage->GetSpacing() );
resampler->SetOutputOrigin( m_View->m_ItkQBallImage->GetOrigin() );
resampler->SetOutputDirection( m_View->m_ItkQBallImage->GetDirection() );
resampler->SetSize( m_View->m_ItkQBallImage->GetLargestPossibleRegion().GetSize() );
// resample mask image
resampler->SetInput( m_View->m_MaskImage );
resampler->SetDefaultPixelValue(0);
resampler->Update();
m_View->m_MaskImage = resampler->GetOutput();
if (m_View->m_GfaImage.IsNotNull())
{
ResamplerType::Pointer resampler = ResamplerType::New();
resampler->SetOutputSpacing( m_View->m_ItkQBallImage->GetSpacing() );
resampler->SetOutputOrigin( m_View->m_ItkQBallImage->GetOrigin() );
resampler->SetOutputDirection( m_View->m_ItkQBallImage->GetDirection() );
resampler->SetSize( m_View->m_ItkQBallImage->GetLargestPossibleRegion().GetSize() );
resampler->SetInput( m_View->m_GfaImage );
resampler->SetDefaultPixelValue(0);
resampler->Update();
m_View->m_GfaImage = resampler->GetOutput();
}
m_View->m_GlobalTracker->SetInput0(m_View->m_ItkQBallImage.GetPointer());
m_View->m_GlobalTracker->SetMaskImage(m_View->m_MaskImage);
m_View->m_GlobalTracker->SetGfaImage(m_View->m_GfaImage);
m_View->m_GlobalTracker->SetTempStart((float)m_View->m_Controls->m_StartTempSlider->value()/100);
m_View->m_GlobalTracker->SetTempEnd((float)m_View->m_Controls->m_EndTempSlider->value()/10000);
m_View->m_GlobalTracker->SetNumIt(m_View->m_Iterations);
m_View->m_GlobalTracker->SetParticleWeight((float)m_View->m_Controls->m_ParticleWeightSlider->value()/10000);
m_View->m_GlobalTracker->SetSubtractMean(m_View->m_Controls->m_MeanSubtractionCheckbox->isChecked());
m_View->m_GlobalTracker->SetParticleWidth((float)(m_View->m_Controls->m_ParticleWidthSlider->value())/10);
m_View->m_GlobalTracker->SetParticleLength((float)(m_View->m_Controls->m_ParticleLengthSlider->value())/10);
m_View->m_GlobalTracker->SetInexBalance((float)m_View->m_Controls->m_InExBalanceSlider->value()/10);
m_View->m_GlobalTracker->SetFiberLength(m_View->m_Controls->m_FiberLengthSlider->value());
m_View->m_GlobalTracker->Update();
m_View->m_TrackingThread.quit();
}
void GibbsTrackingFilter< ItkQBallImageType >::PrepareMaskImage()
{
if(m_MaskImage.IsNull())
{
MITK_INFO << "GibbsTrackingFilter: generating default mask image";
m_MaskImage = ItkFloatImageType::New();
m_MaskImage->SetSpacing( m_QBallImage->GetSpacing() );
m_MaskImage->SetOrigin( m_QBallImage->GetOrigin() );
m_MaskImage->SetDirection( m_QBallImage->GetDirection() );
m_MaskImage->SetRegions( m_QBallImage->GetLargestPossibleRegion() );
m_MaskImage->Allocate();
m_MaskImage->FillBuffer(1.0);
}
else if ( m_MaskImage->GetLargestPossibleRegion().GetSize()[0]!=m_QBallImage->GetLargestPossibleRegion().GetSize()[0] ||
m_MaskImage->GetLargestPossibleRegion().GetSize()[1]!=m_QBallImage->GetLargestPossibleRegion().GetSize()[1] ||
m_MaskImage->GetLargestPossibleRegion().GetSize()[2]!=m_QBallImage->GetLargestPossibleRegion().GetSize()[2] ||
m_MaskImage->GetSpacing()[0]!=m_QBallImage->GetSpacing()[0] ||
m_MaskImage->GetSpacing()[1]!=m_QBallImage->GetSpacing()[1] ||
m_MaskImage->GetSpacing()[2]!=m_QBallImage->GetSpacing()[2] )
{
MITK_INFO << "GibbsTrackingFilter: resampling mask image";
typedef itk::ResampleImageFilter< ItkFloatImageType, ItkFloatImageType, float > ResamplerType;
ResamplerType::Pointer resampler = ResamplerType::New();
resampler->SetOutputSpacing( m_QBallImage->GetSpacing() );
resampler->SetOutputOrigin( m_QBallImage->GetOrigin() );
resampler->SetOutputDirection( m_QBallImage->GetDirection() );
resampler->SetSize( m_QBallImage->GetLargestPossibleRegion().GetSize() );
resampler->SetInput( m_MaskImage );
resampler->SetDefaultPixelValue(0.0);
resampler->Update();
m_MaskImage = resampler->GetOutput();
MITK_INFO << "GibbsTrackingFilter: resampling finished";
}
}
int main(int argc, char** argv )
{
/**
* /home/morgan/invaginatingleg/invagination/invagination_T0.ome.tif
* /home/morgan/invaginatingleg/invagination/invagination_T1.ome.tif
* /home/morgan/invaginatingleg/invagination/invagination_T2.ome.tif
* /home/morgan/invaginatingleg/invagination/invagination_T3.ome.tif
* /home/morgan/invaginationmasked/invaginated_T4.ome.tif
* /home/morgan/invaginationmasked/invaginated_T5.ome.tif
* /home/morgan/invaginationmasked/invaginated_T6.ome.tif
* /home/morgan/invaginationmasked/invaginated_T7.ome.tif
* /home/morgan/invaginationmasked/invaginated_T8.ome.tif
* /home/morgan/invaginationmasked/invaginated_T9.ome.tif
* /home/morgan/invaginationmasked/invaginated_T10.ome.tif
* /home/morgan/invaginationmasked/invaginated_T11.ome.tif
* /home/morgan/invaginationmasked/invaginated_T12.ome.tif
* /home/morgan/invaginationmasked/invaginated_T13.ome.tif
* /home/morgan/invaginationmasked/invaginated_T14.ome.tif
* /home/morgan/invaginationmasked/invaginated_T15.ome.tif
* /home/morgan/invaginationmasked/invaginated_T16.ome.tif
* /home/morgan/invaginationmasked/invaginated_T17.ome.tif
* /home/morgan/invaginationmasked/invaginated_T18.ome.tif
* /home/morgan/invaginationmasked/invaginated_T19.ome.tif
* /home/morgan/invaginationmasked/invaginated_T20.ome.tif
* /home/morgan/invaginationmasked/invaginated_T21.ome.tif
* /home/morgan/invaginationmasked/invaginated_T22.ome.tif
* output
*/
std::vector<std::string> inputFiles;
typedef float PixelType;
// Fixed Image Type
typedef itk::Image<PixelType,3> FixedImageType;
// Moving Image Type
typedef itk::Image<PixelType,3> MovingImageType;
typedef FixedImageType::SpacingType SpacingType;
SpacingType spacing;
//spacing[0]=0.3107403;
//spacing[1]=0.3107403;
//spacing[2]=0.739833;
spacing[0]=0.1569476;
spacing[1]=0.1569476;
spacing[2]=0.6209697;
// Transform Type
typedef itk::VersorRigid3DTransform< double > TransformType;
#if 0
inputFiles.push_back("/home/morgan/invaginatingleg/invagination/invagination_T0.ome.tif");
inputFiles.push_back("/home/morgan/invaginatingleg/invagination/invagination_T1.ome.tif");
typedef float VectorComponentType;
typedef itk::Vector< VectorComponentType, 3 > VectorType;
typedef itk::Image< VectorType, 3 > DeformationFieldType;
typedef itk::ImageFileReader<FixedImageType> FixedImageReaderType;
typedef itk::ImageFileReader<MovingImageType> MovingImageReaderType;
FixedImageReaderType::Pointer fixedImageReader=FixedImageReaderType::New();
MovingImageReaderType::Pointer movingImageReader=MovingImageReaderType::New();
fixedImageReader->SetFileName(inputFiles[0]);
movingImageReader->SetFileName(inputFiles[1]);
fixedImageReader->Update();
movingImageReader->Update();
FixedImageType::Pointer fixedImage = fixedImageReader->GetOutput();
MovingImageType::Pointer movingImage = movingImageReader->GetOutput();
fixedImage->SetSpacing(spacing);
movingImage->SetSpacing(spacing);
typedef itk::VersorRigid3DTransform< double > Rigid3DTransformType;
typedef itk::LandmarkBasedTransformInitializer< Rigid3DTransformType, FixedImageType, MovingImageType >
LandmarkBasedTransformInitializerType;
LandmarkBasedTransformInitializerType::Pointer landmarkBasedTransformInitializer =
LandmarkBasedTransformInitializerType::New();
// Create source and target landmarks.
typedef LandmarkBasedTransformInitializerType::LandmarkPointContainer LandmarkContainerType;
typedef LandmarkBasedTransformInitializerType::LandmarkPointType LandmarkPointType;
LandmarkContainerType fixedLandmarks;
LandmarkContainerType movingLandmarks;
LandmarkPointType fixedPoint;
LandmarkPointType movingPoint;
fixedPoint[0] = 37.510;
fixedPoint[1] = 34.685;
fixedPoint[2] = 52*spacing[2];
movingPoint[0] = 31.468;
movingPoint[1] = 27.593;
movingPoint[2] = 50*spacing[2];
fixedLandmarks.push_back( fixedPoint );
movingLandmarks.push_back( movingPoint );
fixedPoint[0] = 32.645;
fixedPoint[1] = 33.901;
fixedPoint[2] = 52*spacing[2];
movingPoint[0] = 25.888;
movingPoint[1] = 26.353;
movingPoint[2] = 50*spacing[2];
fixedLandmarks.push_back( fixedPoint );
movingLandmarks.push_back( movingPoint );
fixedPoint[0] = 35.784;
fixedPoint[1] = 37.354;
fixedPoint[2] = 52*spacing[2];
movingPoint[0] = 29.918;
movingPoint[1] = 29.918;
movingPoint[2] = 50*spacing[2];
fixedLandmarks.push_back( fixedPoint );
movingLandmarks.push_back( movingPoint );
fixedPoint[0] = 33.116;
fixedPoint[1] = 37.040;
fixedPoint[2] = 52*spacing[2];
movingPoint[0] = 26.818;
movingPoint[1] = 29.608;
movingPoint[2] = 50*spacing[2];
fixedLandmarks.push_back( fixedPoint );
movingLandmarks.push_back( movingPoint );
landmarkBasedTransformInitializer->SetFixedLandmarks( fixedLandmarks );
landmarkBasedTransformInitializer->SetMovingLandmarks( movingLandmarks );
Rigid3DTransformType::Pointer transform = Rigid3DTransformType::New();
transform->SetIdentity();
landmarkBasedTransformInitializer->SetTransform(transform);
landmarkBasedTransformInitializer->InitializeTransform();
typedef itk::ResampleImageFilter<FixedImageType, MovingImageType, double > ResampleFilterType;
ResampleFilterType::Pointer resampleFilter = ResampleFilterType::New();
resampleFilter->SetInput( movingImage );
resampleFilter->SetTransform( transform );
resampleFilter->SetSize( fixedImage->GetLargestPossibleRegion().GetSize() );
resampleFilter->SetOutputOrigin( fixedImage->GetOrigin() );
resampleFilter->SetOutputSpacing( fixedImage->GetSpacing() );
resampleFilter->SetOutputDirection( fixedImage->GetDirection() );
resampleFilter->SetDefaultPixelValue(0);
resampleFilter->GetOutput();
// Write the output
typedef itk::ImageFileWriter< MovingImageType > WriterType;
WriterType::Pointer writer = WriterType::New();
writer->SetInput ( resampleFilter->GetOutput() );
writer->SetFileName( "output.ome.tif" );
writer->Update();
#endif
for(int i=1;i<argc-1;i++){
inputFiles.push_back(argv[i]);
}
std::string outputPrefix=argv[argc-1];
int nImages = inputFiles.size();
std::vector<TransformType::Pointer >transforms(nImages);
for(int i=0;i<nImages;i++){
transforms[i]=TransformType::New();
}
transforms[0]->SetIdentity();
for(int i=1;i<nImages;i++){
/*
* Read files
*/
std::cout << "Registering " << inputFiles[i] <<" to " <<inputFiles[i-1] << std::endl;
typedef itk::ImageFileReader<FixedImageType> FixedImageReaderType;
typedef itk::ImageFileReader<MovingImageType> MovingImageReaderType;
FixedImageReaderType::Pointer fixedImageReader=FixedImageReaderType::New();
MovingImageReaderType::Pointer movingImageReader=MovingImageReaderType::New();
fixedImageReader->SetFileName(inputFiles[i-1]);
movingImageReader->SetFileName(inputFiles[i]);
fixedImageReader->Update();
movingImageReader->Update();
FixedImageType::Pointer fixedImage = fixedImageReader->GetOutput();
MovingImageType::Pointer movingImage = movingImageReader->GetOutput();
fixedImage->SetSpacing(spacing);
movingImage->SetSpacing(spacing);
FixedImageType::SizeType size= fixedImage->GetLargestPossibleRegion().GetSize();
FixedImageType::PointType origin;
origin[0]=size[0]*spacing[0]/2;
origin[1]=size[1]*spacing[1]/2;
origin[2]=size[2]*spacing[2]/2;
fixedImage->SetOrigin(origin);
movingImage->SetOrigin(origin);
RegisterPair<FixedImageType,MovingImageType,TransformType>(fixedImage,movingImage,transforms[i]);
}
typedef itk::ResampleImageFilter<MovingImageType,MovingImageType> ResamplerType;
ResamplerType::Pointer resampler = ResamplerType::New();
//transform->SetIdentity();
#if 0
TransformType::ParametersType initialParameters( transform->GetNumberOfParameters() );
initialParameters.Fill( 0 );
initialParameters[3] = 1.0;
initialParameters[4]=10;
initialParameters[5]=10;
initialParameters[6]=0;
transform->SetParameters(initialParameters);
#endif
for(int i=0;i<nImages;i++){
typedef itk::ImageFileReader<MovingImageType> MovingImageReaderType;
MovingImageReaderType::Pointer movingImageReader=MovingImageReaderType::New();
movingImageReader->SetFileName(inputFiles[i]);
movingImageReader->Update();
MovingImageType::Pointer movingImage = movingImageReader->GetOutput();
movingImage->SetSpacing(spacing);
MovingImageType::SizeType size = movingImage->GetLargestPossibleRegion().GetSize();
MovingImageType::SizeType resampledSize;
resampledSize[0]=1280;
resampledSize[1]=1280;
resampledSize[2]=150;
resampler->SetSize(resampledSize );
typedef itk::TranslationTransform<double,3> TranslationTransformType;
TranslationTransformType::Pointer translationTransform =
TranslationTransformType::New();
TranslationTransformType::OutputVectorType translation;
translation[0] = -20;//-(resampledSize[0]-size[0])/2*spacing[0];
translation[1] = -20;// -(resampledSize[1]-size[1])/2*spacing[1];
translation[2] = -20; //-(resampledSize[2]-size[2])/2*spacing[2];
translationTransform->Translate(translation);
typedef itk::CompositeTransform<double,3> CompositeType;
CompositeType::Pointer composite = CompositeType::New();
composite->AddTransform(translationTransform);
for(int k=1;k<=i;k++){
composite->AddTransform(transforms[k]);
}
std::cout << "Origin: " << movingImage->GetOrigin() << std::endl;
resampler->SetInput(movingImage);
resampler->SetTransform(composite);
resampler->SetOutputOrigin(movingImage->GetOrigin());
resampler->SetOutputSpacing( movingImage->GetSpacing() );
resampler->SetOutputDirection( movingImage->GetDirection() );
resampler->SetDefaultPixelValue( 0 );
resampler->Update();
std::stringstream ss;
ss << outputPrefix << "-T" << i << ".ome.tif";
std::string filename;
ss >> filename;
typedef itk::ImageFileWriter<MovingImageType> MovingImageWriterType;
MovingImageWriterType::Pointer movingImageWriter=MovingImageWriterType::New();
movingImageWriter->SetFileName(filename);
movingImageWriter->SetInput(resampler->GetOutput());
movingImageWriter->Update();
std::cout << transforms[i] << std::endl;
}
}