void gth_chain_copy(GthChain *dest, const GthChain *src)
{
gt_assert(dest&& src);
chain_copy_core(dest, src);
gt_array_add_array(dest->forwardranges, src->forwardranges);
gt_array_add_array(dest->reverseranges, src->reverseranges);
}
static void potentialintronspostpro(GtArray *intronstoprocess,
unsigned long icdelta,
unsigned long icminremintronlength)
{
GtArray *originalintrons;
GtRange potintron;
unsigned long i, potintronlength,
minintronlength = 2 * icdelta + icminremintronlength;
originalintrons = gt_array_new(sizeof (GtRange));
/* save all (potential) introns */
gt_array_add_array(originalintrons, intronstoprocess);
/* reset introns to process */
gt_array_set_size(intronstoprocess, 0);
/* store introns */
for (i = 0; i < gt_array_size(originalintrons); i++) {
potintron = *(GtRange*) gt_array_get(originalintrons, i);
potintronlength = potintron.end - potintron.start + 1;
if (potintronlength >= minintronlength) {
/* keep this intron (plus/minus intron deltas)
that is, this intron is cut out later */
potintron.start += icdelta;
potintron.end -= icdelta;
gt_array_add(intronstoprocess, potintron);
}
/* else: skip this intron
that is, this intron is not cut out later */
}
gt_array_delete(originalintrons);
}
static GtArray *gaeval_visitor_union(GtArray *cov1, GtArray *cov2)
{
agn_assert(cov1 && cov2);
gt_array_add_array(cov1, cov2);
if(gt_array_size(cov1) > 1)
gt_array_sort(cov1, (GtCompare)gt_range_compare);
GtArray *runion = gt_array_new(sizeof(GtRange));
if(gt_array_size(cov1) == 0)
return runion;
GtRange *rng = gt_array_get(cov1, 0);
gt_array_add(runion, *rng);
GtRange *prev = gt_array_get(runion, 0);
if(gt_array_size(cov1) == 1)
return runion;
GtUword i;
for(i = 1; i < gt_array_size(cov1); i++)
{
rng = gt_array_get(cov1, i);
if(gt_range_overlap(rng, prev))
*prev = gt_range_join(rng, prev);
else
{
gt_array_add(runion, *rng);
prev = gt_array_get(runion, gt_array_size(runion) - 1);
}
}
return runion;
}
static GtArray* generic_ranges_uniq_in_place(GtArray *ranges, bool count)
{
GtArray *out_ranges, *count_array;
gt_assert(ranges);
out_ranges = gt_array_new(sizeof (GtRange));
count_array = generic_ranges_uniq(out_ranges, ranges, count);
gt_array_reset(ranges);
gt_array_add_array(ranges, out_ranges); /* XXX: could be more efficient
with something like
gt_array_replace(ranges,
out_ranges) */
gt_array_delete(out_ranges);
return count_array;
}
void gth_backtrace_path_prepend(GthBacktracePath *out_bp,
const GthBacktracePath *in_bp)
{
gt_assert(out_bp && in_bp);
gt_assert(!backtrace_path_start_cutoffs_are_set(out_bp));
gt_assert(!backtrace_path_cutoffs_are_set(in_bp));
gt_assert(in_bp->gen_dp_start + in_bp->gen_dp_length == out_bp->gen_dp_start);
gt_assert(in_bp->ref_dp_start + in_bp->ref_dp_length == out_bp->ref_dp_start);
out_bp->gen_dp_start = in_bp->gen_dp_start;
out_bp->gen_dp_length += in_bp->gen_dp_length;
out_bp->ref_dp_start = in_bp->ref_dp_start;
out_bp->ref_dp_length += in_bp->ref_dp_length;
gt_array_add_array(out_bp->editoperations, in_bp->editoperations);
}
bool gt_tool_iterator_next(GtToolIterator *tool_iterator, const char **name,
GtTool **tool)
{
ToolIterationInfo tii;
gt_assert(tool_iterator && name && tool);
if (gt_array_size(tool_iterator->tool_stack)) {
ToolEntry *entry = gt_array_pop(tool_iterator->tool_stack);
*name = entry->name;
*tool = entry->tool;
if (tool_iterator->prefixptr) {
gt_str_reset(tool_iterator->prefixptr);
if (entry->prefix) {
gt_str_append_str(tool_iterator->prefixptr, entry->prefix);
gt_str_append_char(tool_iterator->prefixptr, tool_iterator->prefixsep);
}
}
if (gt_tool_is_toolbox(entry->tool)) {
GtToolbox *toolbox;
GtArray *toollist;
GtStr *myprefix;
myprefix =
gt_str_new_cstr(entry->prefix ? gt_str_get(entry->prefix) : "");
gt_str_append_cstr(myprefix, entry->name);
toolbox = gt_tool_get_toolbox(entry->tool);
toollist = gt_array_new(sizeof (ToolEntry));
tii.arr = toollist;
tii.str = myprefix;
gt_toolbox_iterate(toolbox, add_tool_to_stack, &tii);
if (gt_array_size(toollist)) {
gt_array_reverse(toollist); /* alphabetical order */
gt_array_add_array(tool_iterator->tool_stack, toollist);
}
gt_array_delete(toollist);
gt_str_delete(myprefix);
} else
gt_str_delete(entry->prefix);
return true;
}
else
return false;
}
int gt_range_unit_test(GtError *err)
{
static GtRange ranges_in[] = { { 620432, 620536 }, { 620432, 620536 },
{ 620957, 621056 }, { 620957, 621056 },
{ 625234, 625253 }, { 625500, 625655 },
{ 625533, 625655 }, { 625533, 625655 },
{ 627618, 627729 }, { 627618, 627729 },
{ 627618, 627729 }, { 662083, 662194 },
{ 662083, 662194 }, { 662083, 662194 },
{ 663032, 663166 }, { 663032, 663166 },
{ 663032, 663166 }, { 664782, 664906 },
{ 664782, 664906 }, { 664782, 664906 },
{ 665748, 665823 }, { 665748, 665823 },
{ 665748, 665823 }, { 666825, 666881 },
{ 666825, 666881 }, { 667797, 667954 },
{ 667845, 667954 }, { 667845, 667954 },
{ 679175, 679280 }, { 679175, 679280 },
{ 679175, 679280 }, { 680427, 680540 },
{ 680427, 680540 }, { 680427, 680540 },
{ 684144, 684293 }, { 684144, 684293 },
{ 684144, 684293 }, { 724903, 724985 },
{ 724903, 724985 }, { 727099, 727325 },
{ 727099, 727325 }, { 732544, 732821 },
{ 732544, 732821 }, { 750016, 750280 },
{ 750016, 750280 }, { 769508, 769734 },
{ 769508, 769734 } },
ranges_out[] = { { 620432, 620536 }, { 620957, 621056 },
{ 625234, 625253 }, { 625500, 625655 },
{ 625533, 625655 }, { 627618, 627729 },
{ 662083, 662194 }, { 663032, 663166 },
{ 664782, 664906 }, { 665748, 665823 },
{ 666825, 666881 }, { 667797, 667954 },
{ 667845, 667954 }, { 679175, 679280 },
{ 680427, 680540 }, { 684144, 684293 },
{ 724903, 724985 }, { 727099, 727325 },
{ 732544, 732821 }, { 750016, 750280 },
{ 769508, 769734 }};
GtUword counts[] = { 2, 2, 1, 1, 2, 3, 3, 3, 3, 3, 2, 1, 2, 3, 3, 3, 2,
2, 2, 2, 2 };
GtArray *ranges, *tmp_ranges, *ctr;
GtUword i;
int had_err = 0;
gt_error_check(err);
gt_ensure(sizeof (ranges_out) / sizeof (ranges_out[0]) ==
sizeof (counts) / sizeof (counts[0]));
/* test gt_ranges_uniq() */
ranges = gt_array_new(sizeof (GtRange));
tmp_ranges = gt_array_new(sizeof (GtRange));
for (i = 0;
i < sizeof (ranges_in) / sizeof (ranges_in[0]) && !had_err;
i++)
gt_array_add(ranges, ranges_in[i]);
gt_ranges_uniq(tmp_ranges, ranges);
gt_ensure(gt_array_size(ranges) ==
sizeof (ranges_in) / sizeof (ranges_in[0]));
gt_ensure(gt_array_size(tmp_ranges) ==
sizeof (ranges_out) / sizeof (ranges_out[0]));
for (i = 0; i < gt_array_size(tmp_ranges) && !had_err; i++) {
gt_ensure(ranges_out[i].start ==
(*(GtRange*) gt_array_get(tmp_ranges, i)).start);
gt_ensure(ranges_out[i].end ==
(*(GtRange*) gt_array_get(tmp_ranges, i)).end);
}
/* test gt_ranges_uniq_in_place() */
gt_array_reset(tmp_ranges);
gt_array_add_array(tmp_ranges, ranges);
gt_ranges_uniq_in_place(tmp_ranges);
for (i = 0; i < gt_array_size(tmp_ranges) && !had_err; i++) {
gt_ensure(ranges_out[i].start ==
(*(GtRange*) gt_array_get(tmp_ranges, i)).start);
gt_ensure(ranges_out[i].end ==
(*(GtRange*) gt_array_get(tmp_ranges, i)).end);
}
/* test gt_ranges_uniq_count() */
gt_array_reset(tmp_ranges);
ctr = gt_ranges_uniq_count(tmp_ranges, ranges);
gt_ensure(gt_array_size(tmp_ranges) == gt_array_size(ctr));
gt_ensure(
gt_array_size(ctr) == sizeof (counts) / sizeof (counts[0]));
for (i = 0; i < gt_array_size(ctr) && !had_err; i++) {
gt_ensure(counts[i] == *(GtUword*) gt_array_get(ctr, i));
gt_ensure(ranges_out[i].start ==
(*(GtRange*) gt_array_get(tmp_ranges, i)).start);
gt_ensure(ranges_out[i].end ==
(*(GtRange*) gt_array_get(tmp_ranges, i)).end);
}
gt_array_delete(ctr);
/* test gt_ranges_uniq_in_place_count() */
ctr = gt_ranges_uniq_in_place_count(ranges);
gt_ensure(gt_array_size(ranges) == gt_array_size(ctr));
gt_ensure(
gt_array_size(ctr) == sizeof (counts) / sizeof (counts[0]));
for (i = 0; i < gt_array_size(ctr) && !had_err; i++) {
gt_ensure(counts[i] == *(GtUword*) gt_array_get(ctr, i));
gt_ensure(
ranges_out[i].start == (*(GtRange*)
gt_array_get(ranges, i)).start);
gt_ensure(
ranges_out[i].end == (*(GtRange*) gt_array_get(ranges, i)).end);
}
gt_array_delete(ctr);
/* test gt_range_reorder() */
if (!had_err) {
GtRange range = { 1, 100 };
range = gt_range_reorder(range);
gt_ensure(range.start == 1 && range.end == 100);
range.start = 100;
range.end = 1;
range = gt_range_reorder(range);
gt_ensure(range.start == 1 && range.end == 100);
}
/* free */
gt_array_delete(ranges);
gt_array_delete(tmp_ranges);
return had_err;
}
static void ensure_eop_of_len_1_before_introns(GtArray *editoperations)
{
Editoperation eop, *eopptr;
Eoptype eoptype;
unsigned long eoplength;
GtArray *backup;
bool processing_necessary = false,
split_match = false;
/* check if processing is necessary
the check is rather simple, it might be possible that
``processing_necessary'' is set to ``true'' whereas in fact no processing
is necessary */
for (eopptr = gt_array_get_space(editoperations);
eopptr < (Editoperation*) gt_array_get_space(editoperations) +
gt_array_size(editoperations) - 1;
eopptr++) {
if ((eoptype = gt_editoperation_type(*eopptr, true)) ==
EOP_TYPE_INTRON_WITH_1_BASE_LEFT ||
eoptype == EOP_TYPE_INTRON_WITH_2_BASES_LEFT) {
processing_necessary = true;
break;
}
}
if (processing_necessary) {
/* init backup for the editoperations */
backup = gt_array_new(sizeof (Editoperation));
/* fill backup */
gt_array_add_array(backup, editoperations);
/* reset the original edit operations */
gt_array_set_size(editoperations, 0);
/* process the backup and fill the original editoperations */
for (eopptr = gt_array_get_space(backup);
eopptr < (Editoperation*)
gt_array_get_space(backup) + gt_array_size(backup);
eopptr++) {
if ((eoptype = gt_editoperation_length(*eopptr, true)) ==
EOP_TYPE_INTRON_WITH_1_BASE_LEFT ||
eoptype == EOP_TYPE_INTRON_WITH_2_BASES_LEFT) {
split_match = true;
}
else if (split_match) {
if (eoptype == EOP_TYPE_MATCH) {
split_match = false;
if ((eoplength = gt_editoperation_length(*eopptr, true)) > 1) {
eop = 1;
gt_array_add(editoperations, eop);
eop = eoplength - 1;
gt_array_add(editoperations, eop);
continue;
}
}
else if (eoptype == EOP_TYPE_MISMATCH ||
eoptype == EOP_TYPE_MISMATCH_WITH_1_GAP) {
split_match = false;
}
}
gt_array_add(editoperations, *eopptr);
}
/* free backup */
gt_array_delete(backup);
}
}
static double gaeval_visitor_calculate_integrity(AgnGaevalVisitor *v,
GtFeatureNode *genemodel,
double coverage,
double *components,
GtError *error)
{
agn_assert(v && genemodel);
GtStr *seqid = gt_genome_node_get_seqid((GtGenomeNode *)genemodel);
GtRange mrna_range = gt_genome_node_get_range((GtGenomeNode *)genemodel);
GtArray *overlapping = gt_array_new( sizeof(GtFeatureNode *) );
bool hasseqid;
gt_feature_index_has_seqid(v->alignments, &hasseqid, gt_str_get(seqid),error);
if(hasseqid)
{
gt_feature_index_get_features_for_range(v->alignments, overlapping,
gt_str_get(seqid), &mrna_range,
error);
}
GtArray *gaps = gt_array_new( sizeof(GtFeatureNode *) );
while(gt_array_size(overlapping) > 0)
{
GtFeatureNode *alignment = *(GtFeatureNode **)gt_array_pop(overlapping);
GtArray *agaps = agn_typecheck_select(alignment,
gaeval_visitor_typecheck_gap);
gt_array_add_array(gaps, agaps);
gt_array_delete(agaps);
}
gt_array_delete(overlapping);
GtUword utr5p_len = agn_mrna_5putr_length(genemodel);
double utr5p_score = 0.0;
if(utr5p_len >= v->params.exp_5putr_len)
utr5p_score = 1.0;
else
utr5p_score = (double)utr5p_len / (double)v->params.exp_5putr_len;
GtUword utr3p_len = agn_mrna_3putr_length(genemodel);
double utr3p_score = 0.0;
if(utr3p_len >= v->params.exp_3putr_len)
utr3p_score = 1.0;
else
utr3p_score = (double)utr3p_len / (double)v->params.exp_3putr_len;
GtArray *introns = agn_typecheck_select(genemodel, agn_typecheck_intron);
GtUword exoncount = agn_typecheck_count(genemodel, agn_typecheck_exon);
agn_assert(gt_array_size(introns) == exoncount - 1);
double structure_score = 0.0;
if(gt_array_size(introns) == 0)
{
GtUword cdslen = agn_mrna_cds_length(genemodel);
if(cdslen >= v->params.exp_cds_len)
structure_score = 1.0;
else
structure_score = (double)cdslen / (double)v->params.exp_cds_len;
}
else
{
structure_score = gaeval_visitor_introns_confirmed(introns, gaps);
}
gt_array_delete(gaps);
gt_array_delete(introns);
double integrity = (v->params.alpha * structure_score) +
(v->params.beta * coverage) +
(v->params.gamma * utr5p_score) +
(v->params.epsilon * utr3p_score);
if(components != NULL)
{
components[0] = structure_score;
components[1] = coverage;
components[2] = utr5p_score;
components[3] = utr3p_score;
}
return integrity;
}
