Пример #1
0
	void read(int argc, char **argv)
	{

		parser.setCommandLine(argc, argv);

		int general_section = parser.addSection("General options");
	    fn1 = parser.getOption("--i1", "1st input STAR file ");
	    fn2 = parser.getOption("--i2", "2nd input STAR file ");
	    fn_both = parser.getOption("--both", "Output STAR file with entries from both input STAR files ", "");
	    fn_only1 = parser.getOption("--only1", "Output STAR file with entries that only occur in the 1st input STAR files ", "");
	    fn_only2 = parser.getOption("--only2", "Output STAR file with entries that only occur in the 2nd input STAR files ", "");
	    fn_label1 = parser.getOption("--label1", "1st metadata label for the comparison (may be string, int or DOUBLE)", "");
	    fn_label2 = parser.getOption("--label2", "2nd metadata label for the comparison (DOUBLE only) for 2D/3D-distance)", "");
	    fn_label3 = parser.getOption("--label3", "3rd metadata label for the comparison (DOUBLE only) for 3D-distance)", "");
	    eps = textToFloat(parser.getOption("--max_dist", "Maximum distance to consider a match (for int and DOUBLE only)", "0."));

      	// Check for errors in the command-line option
    	if (parser.checkForErrors())
    		REPORT_ERROR("Errors encountered on the command line, exiting...");
	}
Пример #2
0
	void read(int argc, char **argv)
	{

		parser.setCommandLine(argc, argv);

		int general_section = parser.addSection("General options");
	    fn_star = parser.getOption("--i", "Input STAR file with the images (as rlnImageName) to be saved in a stack");
	    fn_root = parser.getOption("--o", "Output rootname","output");
	    do_spider  = parser.checkOption("--spider_format", "Write out in SPIDER stack format (by default MRC stack format)");
	    do_split_per_micrograph = parser.checkOption("--split_per_micrograph", "Write out separate stacks for each micrograph (needs rlnMicrographName in STAR file)");
	    do_apply_trans = parser.checkOption("--apply_transformation", "Apply the inplane-transformations (needs _rlnOriginX/Y and _rlnAnglePsi in STAR file)");

	    fn_ext = (do_spider) ? ".spi" : ".mrcs";

      	// Check for errors in the command-line option
    	if (parser.checkForErrors())
    		REPORT_ERROR("Errors encountered on the command line, exiting...");
	}
Пример #3
0
	void read(int argc, char **argv)
	{

		parser.setCommandLine(argc, argv);

		int general_section = parser.addSection("General Options");
		fn_unt = parser.getOption("--u", "STAR file with the untilted xy-coordinates");
		fn_til = parser.getOption("--t", "STAR file with the untilted xy-coordinates");
		size = textToInteger(parser.getOption("--size", "Largest dimension of the micrograph (in pixels), e.g. 4096"));
		dim = textToInteger(parser.getOption("--dim", "Dimension of boxed particles (for EMAN .box files in pixels)", "200"));
		acc = textToFloat(parser.getOption("--acc", "Allowed accuracy (in pixels), e.g. half the particle diameter"));
		tilt = textToFloat(parser.getOption("--tilt", "Fix tilt angle (in degrees)", "99999."));
		rot = textToFloat(parser.getOption("--rot", "Fix direction of the tilt axis (in degrees), 0 = along y, 90 = along x", "99999."));
		do_opt = !parser.checkOption("--dont_opt", "Skip optimization of the transformation matrix");
		mind2 = ROUND(acc * acc);

		int angle_section = parser.addSection("Specified tilt axis and translational search ranges");
		tilt0 = textToFloat(parser.getOption("--tilt0", "Minimum tilt angle (in degrees)","0."));
		tiltF = textToFloat(parser.getOption("--tiltF", "Maximum tilt angle (in degrees)","99999."));
		if (tiltF == 99999.) tiltF = tilt0;
		tiltStep = textToFloat(parser.getOption("--tiltStep", "Tilt angle step size (in degrees)","1."));

		rot0 = textToFloat(parser.getOption("--rot0", "Minimum rot angle (in degrees)","0."));
		rotF = textToFloat(parser.getOption("--rotF", "Maximum rot angle (in degrees)","99999."));
		if (rotF == 99999.) rotF = rot0;
		rotStep = textToFloat(parser.getOption("--rotStep", "Rot angle step size (in degrees)","1."));

		x0 = textToInteger(parser.getOption("--x0", "Minimum X offset (pixels)","-99999"));
		xF = textToInteger(parser.getOption("--xF", "Maximum X offset (pixels)","99999"));
		xStep = textToInteger(parser.getOption("--xStep", "X offset step size (pixels)","-1"));
		y0 = textToInteger(parser.getOption("--y0", "Minimum Y offset (pixels)","-99999"));
		yF = textToInteger(parser.getOption("--yF", "Maximum Y offset (pixels)","99999"));
		yStep = textToInteger(parser.getOption("--yStep", "Y offset step size (pixels)","-1"));

       	// Check for errors in the command-line option
    	if (parser.checkForErrors())
    		REPORT_ERROR("Errors encountered on the command line, exiting...");

		// If tilt and rot were given: do not search those
		if (tilt != 99999.)
		{
			tilt0 = tiltF = tilt;
			tiltStep = 1.;
		}
		if (rot != 99999.)
		{
			rot0 = rotF = rot;
			rotStep = 1.;
		}

		// By default search the entire micrograph
		x0 = XMIPP_MAX(x0, -size);
		xF = XMIPP_MIN(xF, size);
		// By default use a xStep of one third the accuracy
		if (xStep < 0)
			xStep = acc / 3;

		// By default treat y search in the same way as the x-search
		if (y0 == -99999)
			y0 = x0;
		if (yF == 99999)
			yF = xF;
		if (yStep < 0)
			yStep = xStep;

		// Done reading, now fill p_unt and p_til
		MDunt.read(fn_unt);
		MDtil.read(fn_til);

		// Check for the correct labels
		if (!MDunt.containsLabel(EMDL_IMAGE_COORD_X) || !MDunt.containsLabel(EMDL_IMAGE_COORD_Y))
			REPORT_ERROR("ERROR: Untilted STAR file does not contain the rlnCoordinateX or Y labels");
		if (!MDtil.containsLabel(EMDL_IMAGE_COORD_X) || !MDtil.containsLabel(EMDL_IMAGE_COORD_Y))
			REPORT_ERROR("ERROR: Tilted STAR file does not contain the rlnCoordinateX or Y labels");

		double x, y;

		p_unt.clear();
		FOR_ALL_OBJECTS_IN_METADATA_TABLE(MDunt)
		{
			MDunt.getValue(EMDL_IMAGE_COORD_X, x);
			MDunt.getValue(EMDL_IMAGE_COORD_Y, y);
			p_unt.push_back((int)x);
			p_unt.push_back((int)y);
		}
		p_til.clear();
		FOR_ALL_OBJECTS_IN_METADATA_TABLE(MDtil)
		{
			MDtil.getValue(EMDL_IMAGE_COORD_X, x);
			MDtil.getValue(EMDL_IMAGE_COORD_Y, y);
			p_til.push_back((int)x);
			p_til.push_back((int)y);
		}

		// Initialize best transformation params
		best_x = best_y = 9999;
		best_rot = best_tilt = 9999.;

	}
Пример #4
0
	void usage()
	{
		parser.writeUsage(std::cerr);
	}
Пример #5
0
	void read(int argc, char **argv)
	{

		parser.setCommandLine(argc, argv);

		int general_section = parser.addSection("General options");
	    fn_unt = parser.getOption("--u", "Input STAR file with untilted particles");
	    fn_til = parser.getOption("--t", "Input STAR file with tilted particles");
	    fn_eps = parser.getOption("--o", "Output EPS file ", "tiltpair.eps");
	    fn_sym = parser.getOption("--sym", "Symmetry point group", "C1");
	    exp_tilt = textToFloat(parser.getOption("--exp_tilt", "Choose symmetry operator that gives tilt angle closest to this value", "0."));
	    exp_beta = textToFloat(parser.getOption("--exp_beta", "Choose symmetry operator that gives beta angle closest to this value", "0."));
	    dist_from_alpha = textToFloat(parser.getOption("--dist_from_alpha", "Direction (alpha angle) of tilt axis from which to calculate distance", "0."));
	    dist_from_tilt = textToFloat(parser.getOption("--dist_from_tilt", "Tilt angle from which to calculate distance", "0."));
	    plot_max_tilt = textToFloat(parser.getOption("--max_tilt", "Maximum tilt angle to plot in the EPS file", "90."));
	    plot_spot_radius = textToInteger(parser.getOption("--spot_radius", "Radius in pixels of the spots in the tiltpair plot", "3"));

      	// Check for errors in the command-line option
    	if (parser.checkForErrors())
    		REPORT_ERROR("Errors encountered on the command line, exiting...");
	}