bool BamProcessor::finalize (bool success)
{
if (outfile_.IsOpen ())
{
trclog << "Closing output file" << std::endl;
outfile_.Close ();
}
if (logfile_.is_open ())
{
time_t t = time (NULL);
print_stats (logfile_);
logfile_ << "\nFinished " << (success ? "successfully" : "due to error") << " at " << asctime (localtime (&t)) << "\n";
trclog << "Closing log file" << std::endl;
logfile_.close ();
}
if (info.enabled ())
print_stats (info.o_);
return true;
}
bool BamProcessor::process ()
{
if (!infile_.ReadHeader (sam_header_))
ers << "Unable to read SAM header" << Throw;
else
info << "Header read" << std::endl;
if (outfile_.IsOpen ())
{
if (!outfile_.WriteHeader (sam_header_))
ers << "Unable to write header data" << Throw;
else
info << "Header written" << std::endl;
}
// set up signal handlers
sighandler_t sighandler_int, sighandler_term, sighandler_hup;
// set INT handler to int_handler if interrupting is not disabled allready
if ((sighandler_int = signal (SIGINT, int_handler)) == SIG_IGN)
signal (SIGINT, SIG_IGN), sighandler_int = NULL;
// set HUP handler to nothing
sighandler_hup = signal (SIGHUP, SIG_IGN);
// set TERM handler to int_handler if terminating is not disabled allready
if ((sighandler_term = signal (SIGTERM, int_handler)) == SIG_IGN)
signal (SIGTERM, SIG_IGN), sighandler_term = NULL;
begtime_ = time (NULL);
while (!infile_.IsEOF () && !interrupted)
{
if (limit_ && proc_cnt_ >= limit_)
{
info << limit_ << " records processed. Limit reached." << std::endl;
break;
}
if (read_cnt_ == skip_)
timer_.mark ();
infile_.ReadRecord (sam_header_, rec_);
++ read_cnt_;
if (read_cnt_-1 >= skip_)
{
if (!processRecord ())
++ fail_cnt_;
++ proc_cnt_;
if (outfile_.IsOpen ())
outfile_.WriteRecord (sam_header_, rec_);
}
if (timer_ ())
{
info << "\r" << read_cnt_;
if (proc_cnt_ != read_cnt_)
info << " rd " << proc_cnt_;
info << " pr ";
if (realigned_cnt_ != proc_cnt_)
info << realigned_cnt_ << " al (" << (double (realigned_cnt_) * 100 / proc_cnt_) << "%) ";
info << modified_cnt_ << " mod (" << (double (modified_cnt_) * 100 / proc_cnt_) << "%) ";
if (pos_adjusted_cnt_)
info << pos_adjusted_cnt_ << " sh (" << (double (pos_adjusted_cnt_) * 100 / modified_cnt_) << "% mod) ";
info << "in " << timer_.tot_elapsed () << " sec (" << std::setprecision (3) << std::fixed << timer_.speed () << " r/s)" << std::flush;
}
}
if (interrupted)
{
errlog << "\nProcessing interrupted by ";
switch (signal_received)
{
case SIGTERM:
errlog << "TERM signal";
break;
case SIGINT:
errlog << "user's request";
break;
default:
errlog << "receipt of signal " << signal_received;
}
errlog << std::endl;
}
// restore signal handlers
if (sighandler_term)
signal (SIGTERM, sighandler_term);
if (sighandler_int)
signal (SIGINT, sighandler_int);
if (sighandler_hup)
signal (SIGHUP, sighandler_hup);
return 0;
}
bool BamProcessor::init (const ContalignParams& p)
{
read_cnt_ = proc_cnt_ = toolongs_ = unaligned_cnt_ = fail_cnt_ = nomd_cnt_ = realigned_cnt_ = modified_cnt_ = pos_adjusted_cnt_ = 0;
log_diff_ = log_matr_ = log_base_ = false;
p_ = &p;
if (!*p.inbam ())
ers << "Input file name not specified" << Throw;
limit_ = p.limit ();
skip_ = p.skip ();
infile_.OpenForRead (p.inbam ());
if (!infile_.IsOpen ())
ers << p.inbam () << ThrowEx (FileNotFoundRerror);
bool index_ok = false;
if (*p.bamidx ())
{
index_ok = infile_.ReadBamIndex (p.bamidx ());
if (!index_ok)
warn << "Unable to open specified BAM index: " << p.bamidx () << ". Default index will be attempted" << std::endl;
}
if (!index_ok)
{
try
{
index_ok = infile_.ReadBamIndex ();
}
catch (std::exception& e)
{
// for some reason not converted into return status by libStatGen
}
if (!index_ok)
warn << "Unable to open default BAM index for " << p.inbam () << std::endl;
}
if (*p.refname () || p.refno () != -1)
{
if (!index_ok)
ers << "Reference section specified, but the BAM index could not be open." << Throw;
if (*p.refname ())
{
if (p.endpos () != 0)
{
infile_.SetReadSection (p.refname (), p.begpos (), p.endpos ());
info << "Read section set : " << p.refname () << ": " << p.begpos () << "-" << p.endpos () << std::endl;
}
else
{
infile_.SetReadSection (p.refname ());
info << "Read section set : " << p.refname () << std::endl;
}
}
else
{
if (p.endpos () != 0)
{
info << "Read section set : ref# " << p.refno () << ": " << p.begpos () << "-" << p.endpos () << std::endl;
infile_.SetReadSection (p.refno (), p.begpos (), p.endpos ());
}
else
{
info << "Read section set : ref# " << p.refno () << std::endl;
infile_.SetReadSection (p.refno ());
}
}
}
if (*p.outbam ())
{
if (!p.overwrite () && file_exists (p.outbam ()))
ers << "Output file " << p.outbam () << " exists. Use --ov key to allow overwriting" << Throw;
outfile_.OpenForWrite (p.outbam ());
if (!outfile_.IsOpen ())
ers << "Unable to open output file " << p.outbam () << std::endl;
}
if (*p.logfname ())
{
if (!p.overwrite () && file_exists (p.logfname ()))
ers << "Log file " << p.logfname () << " exists. Use --ov key to allow overwriting" << Throw;
logfile_.open (p.logfname (), std::fstream::out);
if (!logfile_.is_open ())
ers << "Unable to open log file " << p.logfname () << std::endl;
time_t t = time (NULL);
logfile_ << "Context-aware realigner log\nStarted at " << asctime (localtime (&t)) << "\nParameters:\n";
logfile_ << *(p.parameters_);
logfile_ << std::endl;
log_base_ = p.logging ("base");
log_diff_ = p.logging ("diff");
log_matr_ = p.logging ("matr");
}
band_width_ = p.bwid ();
switch (p.algo ())
{
case ContalignParams::TEMPL:
{
matrix_.configure (genstr::nucleotides.symbols (), genstr::nucleotides.size (), genstr::NegUnitaryMatrix <int, 4>().values ());
gap_cost_.configure (p.gip (), p.gep ());
taligner_.configure (&matrix_, &gap_cost_, &gap_cost_, &genstr::nn2num, &genstr::nn2num);
}
break;
case ContalignParams::PLAIN:
{
batches_.reset (max_batch_no_);
aligner_.init (MAX_SEQ_LEN, MAX_SEQ_LEN*MAX_BAND_WIDTH, p.gip (), p.gep (), p.mat (), -p.mis ());
if (log_matr_)
aligner_.set_log (logfile_);
if (p.debug () > 5)
aligner_.set_trace (true);
}
break;
case ContalignParams::POLY:
{
batches_.reset (max_batch_no_);
contalign_.init (MAX_SEQ_LEN, MAX_RSEQ_LEN, MAX_SEQ_LEN*MAX_BAND_WIDTH, p.gip (), p.gep (), p.mat (), -p.mis ());
if (log_matr_)
contalign_.set_log (logfile_);
if (p.debug () > 5)
contalign_.set_trace (true);
}
break;
default:
{
ers << "Alignment algorithm " << p.algostr () << " not yet supported" << Throw;
}
}
timer_.reset (DEFAULT_REPORT_IVAL, 1);
return true;
}
