/** * FASTQ format specification: http://maq.sourceforge.net/fastq.shtml */ static void load(IOAdapter* io, const U2DbiRef& dbiRef, const QVariantMap& hints, const GUrl& docUrl, QList<GObject*>& objects, U2OpStatus& os, int gapSize, int predictedSize, QString& writeLockReason) { DbiOperationsBlock opBlock(dbiRef, os); CHECK_OP(os, ); Q_UNUSED(opBlock); writeLockReason.clear(); bool merge = gapSize!=-1; QByteArray sequence; QByteArray qualityScores; QStringList headers; QSet<QString> uniqueNames; QVector<U2Region> mergedMapping; QByteArray gapSequence((merge ? gapSize : 0), 0); sequence.reserve(predictedSize); qualityScores.reserve(predictedSize); // for lower case annotations GObjectReference sequenceRef; qint64 sequenceStart = 0; U2SequenceImporter seqImporter(hints, true); const QString folder = hints.value(DocumentFormat::DBI_FOLDER_HINT, U2ObjectDbi::ROOT_FOLDER).toString(); int seqNumber = 0; int progressUpNum = 0; const int objectsCountLimit = hints.contains(DocumentReadingMode_MaxObjectsInDoc) ? hints[DocumentReadingMode_MaxObjectsInDoc].toInt() : -1; const bool settingsMakeUniqueName = !hints.value(DocumentReadingMode_DontMakeUniqueNames, false).toBool(); while (!os.isCoR()) { //read header QString sequenceName = readSequenceName(os, io, '@'); // check for eof while trying to read another FASTQ block if (io->isEof()) { break; } CHECK_OP_BREAK(os); if(sequenceName.isEmpty()){ sequenceName = "Sequence"; } if ((merge == false) || (seqNumber == 0)) { QString objName = sequenceName; if (settingsMakeUniqueName) { objName = (merge) ? "Sequence" : TextUtils::variate(sequenceName, "_", uniqueNames); objName.squeeze(); uniqueNames.insert(objName); } seqImporter.startSequence(dbiRef, folder, objName, false, os); CHECK_OP_BREAK(os); } //read sequence if (merge && sequence.length() > 0) { seqImporter.addDefaultSymbolsBlock(gapSize,os); sequenceStart += sequence.length(); sequenceStart+=gapSize; CHECK_OP_BREAK(os); } sequence.clear(); readSequence(os, io, sequence); MemoryLocker lSequence(os, qCeil(sequence.size()/(1000*1000))); CHECK_OP_BREAK(os); Q_UNUSED(lSequence); seqImporter.addBlock(sequence.data(),sequence.length(),os); CHECK_OP_BREAK(os); QString qualSequenceName = readSequenceName(os, io, '+'); if (!qualSequenceName.isEmpty()) { static const QString err = U2::FastqFormat::tr("Not a valid FASTQ file: %1, sequence name differs from quality scores name: %2 and %3"); CHECK_EXT_BREAK(sequenceName == qualSequenceName, os.setError(err.arg(docUrl.getURLString()).arg(sequenceName).arg(qualSequenceName))); } // read qualities qualityScores.clear(); readQuality(os, io, qualityScores, sequence.size()); CHECK_OP_BREAK(os); static const QString err = U2::FastqFormat::tr("Not a valid FASTQ file: %1. Bad quality scores: inconsistent size.").arg(docUrl.getURLString()); CHECK_EXT_BREAK(sequence.length() == qualityScores.length(), os.setError(err)); seqNumber++; progressUpNum++; if (merge) { headers.append(sequenceName); mergedMapping.append(U2Region(sequenceStart, sequence.length() )); } else { if (objectsCountLimit > 0 && objects.size() >= objectsCountLimit) { os.setError(FastqFormat::tr("File \"%1\" contains too many sequences to be displayed. " "However, you can process these data using instruments from the menu <i>Tools -> NGS data analysis</i> " "or pipelines built with Workflow Designer.") .arg(io->getURL().getURLString())); break; } U2Sequence u2seq = seqImporter.finalizeSequenceAndValidate(os); CHECK_OP_BREAK(os); sequenceRef = GObjectReference(io->getURL().getURLString(), u2seq.visualName, GObjectTypes::SEQUENCE, U2EntityRef(dbiRef, u2seq.id)); U2SequenceObject* seqObj = new U2SequenceObject(u2seq.visualName, U2EntityRef(dbiRef, u2seq.id)); CHECK_EXT_BREAK(seqObj != NULL, os.setError("U2SequenceObject is NULL")); seqObj->setQuality(DNAQuality(qualityScores)); objects << seqObj; U1AnnotationUtils::addAnnotations(objects, seqImporter.getCaseAnnotations(), sequenceRef, NULL, hints); } if (PROGRESS_UPDATE_STEP == progressUpNum) { progressUpNum = 0; os.setProgress(io->getProgress()); } } CHECK_OP_EXT(os, qDeleteAll(objects); objects.clear(), ); bool emptyObjects = objects.isEmpty(); CHECK_EXT(!emptyObjects || merge, os.setError(Document::tr("Document is empty.")), ); SAFE_POINT(headers.size() == mergedMapping.size(), "headers <-> regions mapping failed!", ); if (!merge) { return; } U2Sequence u2seq = seqImporter.finalizeSequenceAndValidate(os); CHECK_OP(os,); sequenceRef = GObjectReference(io->getURL().getURLString(), u2seq.visualName, GObjectTypes::SEQUENCE, U2EntityRef(dbiRef, u2seq.id)); U1AnnotationUtils::addAnnotations(objects, seqImporter.getCaseAnnotations(), sequenceRef, NULL, hints); objects << new U2SequenceObject(u2seq.visualName, U2EntityRef(dbiRef, u2seq.id)); objects << DocumentFormatUtils::addAnnotationsForMergedU2Sequence(sequenceRef, dbiRef, headers, mergedMapping, hints); if (headers.size() > 1) { writeLockReason = DocumentFormat::MERGED_SEQ_LOCK; } }