void UHMMCalibrate::calibrateParallel(WorkPool_s *wpool, TaskStateInfo& si) {
HMMERTaskLocalData *tls = getHMMERTaskLocalData();
struct alphabet_s *al = &tls->al;
struct plan7_s *hmm = wpool->hmm;
struct dpmatrix_s *mx = CreatePlan7Matrix(1, hmm->M, 25, 0);
int len;
float sc;
char *seq;
unsigned char *dsq;
while(true) {
/* generate a sequence */
{
QMutexLocker locker(&wpool->lockInput);
wpool->nseq++;
if (wpool->nseq > wpool->nsample) { /* we're done; release input lock, break loop */
break;
}
if (wpool->fixedlen) {
len = wpool->fixedlen;
} else {
do {
len = (int) Gaussrandom(wpool->lenmean, wpool->lensd);
} while (len < 1);
}
seq = RandomSequence(al->Alphabet, wpool->randomseq.data(), al->Alphabet_size, len);
}
/* compute score */
dsq = DigitizeSequence(seq, len);
if (P7ViterbiSpaceOK(len, hmm->M, mx)) {
sc = P7Viterbi(dsq, len, hmm, mx, NULL);
} else {
int pStub;
sc = P7SmallViterbi(dsq, len, hmm, mx, NULL, pStub);
}
free(dsq);
free(seq);
/* save output */
QMutexLocker locker(&wpool->lockOutput);
AddToHistogram(wpool->hist, sc);
wpool->max_score = qMax(wpool->max_score, sc);
si.progress = int(100*wpool->nseq/float(wpool->nsample)); //TODO: update progress for all tasks?
if (wpool->progress!=NULL) {
*wpool->progress = si.progress;
}
}
FreePlan7Matrix(mx);
}
/* Function: main_loop_serial()
* Date: SRE, Tue Aug 18 16:18:28 1998 [St. Louis]
*
* Purpose: Given an HMM and parameters for synthesizing random
* sequences; return a histogram of scores.
* (Serial version)
*
* Args: hmm - an HMM to calibrate.
* seed - random number seed
* nsample - number of seqs to synthesize
* lenmean - mean length of random sequence
* lensd - std dev of random seq length
* fixedlen - if nonzero, override lenmean, always this len
* ret_hist - RETURN: the score histogram
* ret_max - RETURN: highest score seen in simulation
*
* Returns: (void)
* hist is alloc'ed here, and must be free'd by caller.
*/
static void
main_loop_serial(struct plan7_s *hmm, int seed, int nsample,
float lenmean, float lensd, int fixedlen,
struct histogram_s **ret_hist, float *ret_max)
{
struct histogram_s *hist;
float randomseq[MAXABET];
float p1;
float max;
char *seq;
char *dsq;
float score;
int sqlen;
int idx;
/* Initialize.
* We assume we've already set the alphabet (safe, because
* HMM input sets the alphabet).
*/
sre_srandom(seed);
P7Logoddsify(hmm, TRUE);
P7DefaultNullModel(randomseq, &p1);
hist = AllocHistogram(-200, 200, 100);
max = -FLT_MAX;
for (idx = 0; idx < nsample; idx++)
{
/* choose length of random sequence */
if (fixedlen) sqlen = fixedlen;
else do sqlen = (int) Gaussrandom(lenmean, lensd); while (sqlen < 1);
/* generate it */
seq = RandomSequence(Alphabet, randomseq, Alphabet_size, sqlen);
dsq = DigitizeSequence(seq, sqlen);
if (P7ViterbiSize(sqlen, hmm->M) <= RAMLIMIT)
score = P7Viterbi(dsq, sqlen, hmm, NULL);
else
score = P7SmallViterbi(dsq, sqlen, hmm, NULL);
AddToHistogram(hist, score);
if (score > max) max = score;
free(dsq);
free(seq);
}
*ret_hist = hist;
*ret_max = max;
return;
}
static void
main_loop_serial(struct plan7_s *hmm, const char* seq, int seqLen, struct threshold_s *thresh, int do_forward,
int do_null2, int do_xnu, struct histogram_s *histogram, struct tophit_s *ghit, struct tophit_s *dhit,
int *ret_nseq, TaskStateInfo& ti)
{
//get HMMERTaskLocalData
HMMERTaskLocalData *tld = getHMMERTaskLocalData();
alphabet_s *al = &tld->al;
struct dpmatrix_s *mx; // DP matrix, growable
struct p7trace_s *tr; // traceback
unsigned char *dsq; // digitized target sequence
float sc; // score of an HMM search
double pvalue; // pvalue of an HMM score
double evalue; // evalue of an HMM score
// Create a DP matrix; initially only two rows big, but growable;
// we overalloc by 25 rows (L dimension) when we grow; not growable
// in model dimension, since we know the hmm size
mx = CreatePlan7Matrix(1, hmm->M, 25, 0);
assert(seqLen > 0);
dsq = DigitizeSequence(seq, seqLen);
if (do_xnu && al->Alphabet_type == hmmAMINO) {
XNU(dsq, seqLen);
}
// 1. Recover a trace by Viterbi.
// In extreme cases, the alignment may be literally impossible;
// in which case, the score comes out ridiculously small (but not
// necessarily <= -INFTY, because we're not terribly careful
// about underflow issues), and tr will be returned as NULL.
if (P7ViterbiSpaceOK(seqLen, hmm->M, mx)) {
sc = P7Viterbi(dsq, seqLen, hmm, mx, &tr);
} else {
sc = P7SmallViterbi(dsq, seqLen, hmm, mx, &tr, ti.progress);
}
// 2. If we're using Forward scores, calculate the
// whole sequence score; this overrides anything
// PostprocessSignificantHit() is going to do to the per-seq score.
if (do_forward) {
sc = P7Forward(dsq, seqLen, hmm, NULL);
if (do_null2) sc -= TraceScoreCorrection(hmm, tr, dsq);
}
// 2. Store score/pvalue for global alignment; will sort on score,
// which in hmmsearch is monotonic with E-value.
// Keep all domains in a significant sequence hit.
// We can only make a lower bound estimate of E-value since
// we don't know the final value of nseq yet, so the list
// of hits we keep in memory is >= the list we actually
// output.
//
pvalue = PValue(hmm, sc);
evalue = thresh->Z ? (double) thresh->Z * pvalue : (double) pvalue;
if (sc >= thresh->globT && evalue <= thresh->globE) {
sc = PostprocessSignificantHit(ghit, dhit,
tr, hmm, dsq, seqLen,
(char *)"sequence", //todo: sqinfo.name,
NULL,
NULL,
do_forward, sc,
do_null2,
thresh,
FALSE); // FALSE-> not hmmpfam mode, hmmsearch mode
}
AddToHistogram(histogram, sc);
P7FreeTrace(tr);
free(dsq);
FreePlan7Matrix(mx);
return;
}
int
main(void)
{
struct p7trace_s *tr; /* traceback of an alignment */
int master_tid; /* PVM TID of our master */
char *hmmfile; /* file to read HMM(s) from */
HMMFILE *hmmfp; /* opened hmmfile for reading */
struct plan7_s *hmm;
char *seq;
char *dsq;
int len;
int nhmm; /* number of HMM to work on */
float sc;
int my_idx = -1; /* my index, 0..nslaves-1 */
float globT; /* T parameter: keep only hits > globT bits */
double globE; /* E parameter: keep hits < globE E-value */
double pvalue; /* Z*pvalue = Evalue */
int Z; /* nseq to base E value calculation on */
int send_trace; /* TRUE if score is significant */
int do_xnu; /* TRUE to do XNU filter on seq */
int do_forward; /* TRUE to use Forward() scores not Viterbi */
int do_null2; /* TRUE to correct scores w/ ad hoc null2 */
int alphatype; /* alphabet type, hmmAMINO or hmmNUCLEIC */
int code; /* return code after initialization */
/* Register leave_pvm() cleanup function so any exit() call
* first calls pvm_exit().
*/
if (atexit(leave_pvm) != 0) { pvm_exit(); Die("slave couldn't register leave_pvm()"); }
/*****************************************************************
* initialization.
* Master broadcasts to us:
* 1) len of HMM file name (int)
* 2) name of HMM file (string)
* 3) length of sequence string (int)
* 4) sequence (string)
* 5) globT threshold
* 6) globE threshold
* 7) Z
* 8) do_xnu flag
* 9) do_forward flag
* 10) do_null2 flag
* 11) alphabet type
* We receive the broadcast and open the files.
******************************************************************/
master_tid = pvm_parent(); /* who's our master? */
pvm_recv(master_tid, HMMPVM_INIT);
pvm_upkint(&len, 1, 1);
hmmfile = MallocOrDie(sizeof(char *) * (len+1));
pvm_upkstr(hmmfile);
pvm_upkint(&len, 1, 1);
seq = MallocOrDie(sizeof(char *) * (len+1));
pvm_upkstr(seq);
pvm_upkfloat(&globT, 1, 1);
pvm_upkdouble(&globE, 1, 1);
pvm_upkint(&Z, 1, 1);
pvm_upkint(&do_xnu, 1, 1);
pvm_upkint(&do_forward, 1, 1);
pvm_upkint(&do_null2, 1, 1);
pvm_upkint(&alphatype, 1, 1);
SetAlphabet(alphatype);
/* Open HMM file (maybe in HMMERDB) */
code = HMMPVM_OK;
if ((hmmfp = HMMFileOpen(hmmfile, "HMMERDB")) == NULL)
code = HMMPVM_NO_HMMFILE;
else if (hmmfp->gsi == NULL)
code = HMMPVM_NO_INDEX;
/* report our status.
*/
pvm_initsend(PvmDataDefault);
pvm_pkint(&code, 1, 1);
pvm_send(master_tid, HMMPVM_RESULTS);
dsq = DigitizeSequence(seq, len);
if (do_xnu) XNU(dsq, len);
/*****************************************************************
* Main loop.
* Receive an integer 0..nhmm-1 for which HMM to search against.
* If we receive a -1, we shut down.
*****************************************************************/
for (;;)
{
pvm_recv(master_tid, HMMPVM_WORK);
pvm_upkint(&nhmm, 1, 1);
if (my_idx < 0) my_idx = nhmm; /* first time thru, remember what index we are. */
if (nhmm == -1) break; /* shutdown signal */
/* move to our assigned HMM in the HMM file, and read it
*/
HMMFilePositionByIndex(hmmfp, nhmm);
if (! HMMFileRead(hmmfp, &hmm)) Die("unexpected end of HMM file");
if (hmm == NULL) Die("unexpected failure to parse HMM file");
P7Logoddsify(hmm, TRUE);
/* Score sequence, do alignment (Viterbi), recover trace
*/
if (P7ViterbiSize(len, hmm->M) <= RAMLIMIT)
{
SQD_DPRINTF1(("P7Viterbi(): Estimated size %d Mb\n", P7ViterbiSize(len, hmm->M)));
sc = P7Viterbi(dsq, len, hmm, &tr);
}
else
{
SQD_DPRINTF1(("P7SmallViterbi() called; %d Mb > %d\n", P7ViterbiSize(len, hmm->M), RAMLIMIT));
sc = P7SmallViterbi(dsq, len, hmm, &tr);
}
if (do_forward) sc = P7Forward(dsq, len, hmm, NULL);
if (do_null2) sc -= TraceScoreCorrection(hmm, tr, dsq);
pvalue = PValue(hmm, sc);
send_trace = (sc > globT && pvalue * (float) Z < globE) ? 1 : 0;
/* return output
*/
pvm_initsend(PvmDataDefault);
pvm_pkint(&my_idx, 1, 1); /* tell master who we are */
pvm_pkstr(hmm->name); /* double check that we did the right thing */
pvm_pkfloat(&sc, 1, 1);
pvm_pkdouble(&pvalue, 1, 1);
pvm_pkint(&send_trace, 1, 1); /* flag for whether a trace structure is coming */
if (send_trace) PVMPackTrace(tr);
pvm_send(master_tid, HMMPVM_RESULTS);
/* cleanup
*/
FreePlan7(hmm);
P7FreeTrace(tr);
}
/***********************************************
* Cleanup, return.
***********************************************/
HMMFileClose(hmmfp);
free(seq);
free(dsq);
free(hmmfile);
return 0;
}
static void main_loop_serial(struct plan7_s *hmm, int seed, int nsample,
float lenmean, float lensd, int fixedlen,
struct histogram_s **ret_hist, float *ret_max, int& cancelFlag, int& progress)
{
struct histogram_s *hist;
struct dpmatrix_s *mx;
float randomseq[MAXABET];
float p1;
float max;
char *seq;
unsigned char *dsq;
float score;
int sqlen;
int idx;
// Initialize.
// We assume we've already set the alphabet (safe, because
// HMM input sets the alphabet).
sre_srandom(seed);
//get HMMERTaskLocalData
HMMERTaskLocalData *tls = getHMMERTaskLocalData();
alphabet_s &al = tls->al;
SetAlphabet(hmm->atype);
P7Logoddsify(hmm, TRUE);
P7DefaultNullModel(randomseq, &p1);
hist = AllocHistogram(-200, 200, 100);
mx = CreatePlan7Matrix(1, hmm->M, 25, 0);
max = -FLT_MAX;
progress = 0;
int pStub;
for (idx = 0; idx < nsample && !cancelFlag; idx++) {
// choose length of random sequence
if (fixedlen) {
sqlen = fixedlen;
} else {
do sqlen = (int) Gaussrandom(lenmean, lensd); while (sqlen < 1);
}
// generate it
seq = RandomSequence(al.Alphabet, randomseq, al.Alphabet_size, sqlen);
dsq = DigitizeSequence(seq, sqlen);
if (P7ViterbiSpaceOK(sqlen, hmm->M, mx)) {
score = P7Viterbi(dsq, sqlen, hmm, mx, NULL);
} else {
score = P7SmallViterbi(dsq, sqlen, hmm, mx, NULL, pStub);
}
AddToHistogram(hist, score);
max = qMax(score, max);
progress = int(100*idx/float(nsample));
free(dsq);
free(seq);
}
FreePlan7Matrix(mx);
*ret_hist = hist;
*ret_max = max;
}
int
main(void)
{
int master_tid; /* PVM TID of our master */
int slaveidx; /* my slave index (0..nslaves-1) */
struct plan7_s *hmm; /* HMM to calibrate, sent from master */
struct histogram_s *hist; /* score histogram */
int hmmidx; /* index of this HMM */
char *seq; /* synthetic random sequence */
char *dsq; /* digitized seq */
int len; /* length of seq */
float sc; /* score of seq aligned to HMM */
float max; /* maximum score seen in sample */
int seed; /* random number seed */
int nsample; /* number of seqs to sample */
int fixedlen; /* if nonzero, fixed length of seq */
float lenmean; /* Gaussian mean length of seq */
float lensd; /* Gaussian length std. dev. for seq */
int fitok; /* TRUE if EVD fit was OK */
float randomseq[MAXABET]; /* iid frequencies of residues */
float p1;
int alphatype; /* alphabet type, hmmAMINO or hmmNUCLEIC */
int idx;
int code;
/* Register leave_pvm() cleanup function so any exit() call
* first calls pvm_exit().
*/
if (atexit(leave_pvm) != 0) { pvm_exit(); Die("slave couldn't register leave_pvm()"); }
/*****************************************************************
* initialization.
* Master broadcasts the problem to us: parameters of the
* HMM calibration.
******************************************************************/
master_tid = pvm_parent(); /* who's our master? */
pvm_recv(master_tid, HMMPVM_INIT);
pvm_upkint(&nsample, 1, 1);
pvm_upkint(&fixedlen, 1, 1);
pvm_upkfloat(&lenmean, 1, 1);
pvm_upkfloat(&lensd, 1, 1);
/* tell the master we're OK and ready to go (or not)
*/
code = HMMPVM_OK;
pvm_initsend(PvmDataDefault);
pvm_pkint(&code, 1, 1);
pvm_send(master_tid, HMMPVM_RESULTS);
/*****************************************************************
* Main loop.
* Receive a random number seed, then an HMM to search against.
* If we receive a -1 seed, we shut down.
*****************************************************************/
slaveidx = -1;
for (;;)
{
pvm_recv(master_tid, HMMPVM_WORK);
pvm_upkint(&seed, 1, 1);
if (seed == -1) break; /* shutdown signal */
pvm_upkint(&hmmidx, 1, 1);
pvm_upkint(&alphatype,1, 1);
SetAlphabet(alphatype);
hmm = PVMUnpackHMM();
if (hmm == NULL) Die("oh no, the HMM never arrived");
if (slaveidx == -1) slaveidx = hmmidx;
P7DefaultNullModel(randomseq, &p1);
sre_srandom(seed);
P7Logoddsify(hmm, TRUE);
hist = AllocHistogram(-200, 200, 100);
max = -FLT_MAX;
for (idx = 0; idx < nsample; idx++)
{
/* choose length of random sequence */
if (fixedlen) len = fixedlen;
else do len = (int) Gaussrandom(lenmean, lensd); while (len < 1);
/* generate it */
seq = RandomSequence(Alphabet, randomseq, Alphabet_size, len);
dsq = DigitizeSequence(seq, len);
if (P7ViterbiSize(len, hmm->M) <= RAMLIMIT)
sc = P7Viterbi(dsq, len, hmm, NULL);
else
sc = P7SmallViterbi(dsq, len, hmm, NULL);
AddToHistogram(hist, sc);
if (sc > max) max = sc;
free(seq);
free(dsq);
}
/* Fit an EVD to the observed histogram.
* The TRUE left-censors and fits only the right slope of the histogram.
* The 9999. is an arbitrary high number that means we won't trim outliers
* on the right.
*/
fitok = ExtremeValueFitHistogram(hist, TRUE, 9999.);
/* Return output to master.
* Currently we don't send the histogram back, but we could.
*/
pvm_initsend(PvmDataDefault);
pvm_pkint(&slaveidx, 1, 1);
pvm_pkint(&hmmidx, 1, 1);
PVMPackString(hmm->name);
pvm_pkint(&fitok, 1, 1);
pvm_pkfloat(&(hist->param[EVD_MU]), 1, 1);
pvm_pkfloat(&(hist->param[EVD_LAMBDA]), 1, 1);
pvm_pkfloat(&max, 1, 1);
pvm_send(master_tid, HMMPVM_RESULTS);
/* cleanup
*/
FreeHistogram(hist);
FreePlan7(hmm);
}
/***********************************************
* Cleanup, return.
***********************************************/
return 0; /* pvm_exit() is called by atexit() registration. */
}
/* Function: worker_thread()
* Date: SRE, Thu Jul 16 10:41:02 1998 [St. Louis]
*
* Purpose: The procedure executed by the worker threads.
*
* Args: ptr - (void *) that is recast to a pointer to
* the workpool.
*
* Returns: (void *)
*/
void *
worker_thread(void *ptr)
{
struct plan7_s *hmm;
struct workpool_s *wpool;
char *seq;
char *dsq;
int len;
float sc;
int rtn;
Stopwatch_t thread_watch;
StopwatchStart(&thread_watch);
wpool = (struct workpool_s *) ptr;
hmm = wpool->hmm;
for (;;)
{
/* 1. Synthesize a random sequence.
* The input sequence number is a shared resource,
* and sre_random() isn't thread-safe, so protect
* the whole section with mutex.
*/
/* acquire a lock */
if ((rtn = pthread_mutex_lock(&(wpool->input_lock))) != 0)
Die("pthread_mutex_lock failure: %s\n", strerror(rtn));
/* generate a sequence */
wpool->nseq++;
if (wpool->nseq > wpool->nsample)
{ /* we're done; release input lock, break loop */
if ((rtn = pthread_mutex_unlock(&(wpool->input_lock))) != 0)
Die("pthread_mutex_unlock failure: %s\n", strerror(rtn));
break;
}
if (wpool->fixedlen) len = wpool->fixedlen;
else do len = (int) Gaussrandom(wpool->lenmean, wpool->lensd); while (len < 1);
seq = RandomSequence(Alphabet, wpool->randomseq, Alphabet_size, len);
/* release the lock */
if ((rtn = pthread_mutex_unlock(&(wpool->input_lock))) != 0)
Die("pthread_mutex_unlock failure: %s\n", strerror(rtn));
/* 2. Score the sequence against the model.
*/
dsq = DigitizeSequence(seq, len);
if (P7ViterbiSize(len, hmm->M) <= RAMLIMIT)
sc = P7Viterbi(dsq, len, hmm, NULL);
else
sc = P7SmallViterbi(dsq, len, hmm, NULL);
free(dsq);
free(seq);
/* 3. Save the output; hist and max_score are shared,
* so protect this section with the output mutex.
*/
/* acquire lock on the output queue */
if ((rtn = pthread_mutex_lock(&(wpool->output_lock))) != 0)
Die("pthread_mutex_lock failure: %s\n", strerror(rtn));
/* save output */
AddToHistogram(wpool->hist, sc);
if (sc > wpool->max_score) wpool->max_score = sc;
/* release our lock */
if ((rtn = pthread_mutex_unlock(&(wpool->output_lock))) != 0)
Die("pthread_mutex_unlock failure: %s\n", strerror(rtn));
}
StopwatchStop(&thread_watch);
/* acquire lock on the output queue */
if ((rtn = pthread_mutex_lock(&(wpool->output_lock))) != 0)
Die("pthread_mutex_lock failure: %s\n", strerror(rtn));
/* accumulate cpu time into main stopwatch */
StopwatchInclude(&(wpool->watch), &thread_watch);
/* release our lock */
if ((rtn = pthread_mutex_unlock(&(wpool->output_lock))) != 0)
Die("pthread_mutex_unlock failure: %s\n", strerror(rtn));
pthread_exit(NULL);
return NULL; /* solely to silence compiler warnings */
}
int main(int argc, char **argv)
{
const char *hmmfile; /* file to read HMMs from */
HMMFILE *hmmfp; /* opened hmmfile for reading */
const char *seqfile; /* file to read target sequence from */
char **rseq; /* raw, unaligned sequences */
SQINFO *sqinfo; /* info associated with sequences */
char **dsq; /* digitized raw sequences */
int nseq; /* number of sequences */
char **aseq; /* aligned sequences */
AINFO ainfo; /* alignment information */
float *wgt; /* per-sequence weights */
int i;
struct plan7_s *hmm; /* HMM to align to */
struct p7trace_s **tr; /* traces for aligned sequences */
int be_quiet; /* TRUE to suppress verbose banner */
int matchonly; /* TRUE to show only match state syms */
const char *outfile; /* optional alignment output file */
FILE *ofp; /* handle on alignment output file */
AjPFile ajwithali; /* name of additional alignment file to align */
AjPFile ajmapali; /* name of additional alignment file to map */
AjBool ajmatch=ajFalse;
AjPFile outf=NULL;
AjPStr outfname=NULL;
AjPFile inf=NULL;
AjPStr infname=NULL;
AjPSeqset seqset=NULL;
AjPStr ajseqfile=NULL;
char* mapali=NULL;
char* withali=NULL;
#ifdef MEMDEBUG
unsigned long histid1, histid2, orig_size, current_size;
orig_size = malloc_inuse(&histid1);
fprintf(stderr, "[... memory debugging is ON ...]\n");
#endif
/***********************************************
* Parse command line
***********************************************/
matchonly = FALSE;
outfile = NULL;
be_quiet = FALSE;
withali = NULL;
mapali = NULL;
embInitPV("ohmmalign",argc,argv,"HMMER",VERSION);
ajmatch = ajAcdGetBoolean("matchonly");
if(ajmatch)
matchonly=TRUE;
else
matchonly=FALSE;
ajmapali = ajAcdGetInfile("mapalifile");
if (ajmapali)
mapali = ajCharNewS(ajFileGetNameS(ajmapali));
ajFileClose(&ajmapali);
ajwithali = ajAcdGetInfile("withalifile");
if (ajwithali)
withali = ajCharNewS(ajFileGetNameS(ajwithali));
ajFileClose(&ajwithali);
be_quiet=TRUE;
outf = ajAcdGetOutfile("outfile");
outfname = ajStrNewC((char *)ajFileGetNameC(outf));
if(*ajStrGetPtr(outfname)>31)
ajFileClose(&outf);
outfile = ajStrGetPtr(outfname);
inf = ajAcdGetInfile("hmmfile");
infname = ajStrNewC((char *)ajFileGetNameC(inf));
ajFileClose(&inf);
hmmfile = ajStrGetPtr(infname);
seqset = ajAcdGetSeqset("sequences");
ajseqfile = ajStrNewC(ajStrGetPtr(seqset->Filename));
seqfile = ajStrGetPtr(ajseqfile);
/***********************************************
* Open HMM file (might be in HMMERDB or current directory).
* Read a single HMM from it.
*
* Currently hmmalign disallows the J state and
* only allows one domain per sequence. To preserve
* the S/W entry information, the J state is explicitly
* disallowed, rather than calling a Plan7*Config() function.
* this is a workaround in 2.1 for the 2.0.x "yo!" bug.
***********************************************/
if ((hmmfp = HMMFileOpen(hmmfile, "HMMERDB")) == NULL)
ajFatal("Failed to open HMM file %s\n", hmmfile);
if (!HMMFileRead(hmmfp, &hmm))
ajFatal("Failed to read any HMMs from %s\n", hmmfile);
HMMFileClose(hmmfp);
if (hmm == NULL)
ajFatal("HMM file %s corrupt or in incorrect format? Parse failed", hmmfile);
hmm->xt[XTE][MOVE] = 1.; /* only 1 domain/sequence ("global" alignment) */
hmm->xt[XTE][LOOP] = 0.;
P7Logoddsify(hmm, TRUE);
/* do we have the map we might need? */
if (mapali != NULL && ! (hmm->flags & PLAN7_MAP))
ajFatal("HMMER: HMM file %s has no map; you can't use --mapali.", hmmfile);
/***********************************************
* Open sequence file in current directory.
* Read all seqs from it.
***********************************************/
/*
if (! SeqfileFormat(seqfile, &format, NULL))
switch (squid_errno) {
case SQERR_NOFILE:
ajFatal("Sequence file %s could not be opened for reading", seqfile);
case SQERR_FORMAT:
default:
ajFatal("Failed to determine format of sequence file %s", seqfile);
}
if (! ReadMultipleRseqs(seqfile, format, &rseq, &sqinfo, &nseq))
ajFatal("Failed to read any sequences from file %s", seqfile);
*/
emboss_rseqs(seqset,&rseq,&sqinfo,&nseq);
/***********************************************
* Show the banner
***********************************************/
be_quiet=TRUE;
if (! be_quiet)
{
/* Banner(stdout, banner); */
printf( "HMM file: %s\n", hmmfile);
printf( "Sequence file: %s\n", seqfile);
printf("- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -\n\n");
}
/***********************************************
* Do the work
***********************************************/
/* Allocations and initializations.
*/
dsq = MallocOrDie(sizeof(char *) * nseq);
tr = MallocOrDie(sizeof(struct p7trace_s *) * nseq);
/* Align each sequence to the model, collect traces
*/
for (i = 0; i < nseq; i++)
{
dsq[i] = DigitizeSequence(rseq[i], sqinfo[i].len);
if (P7ViterbiSize(sqinfo[i].len, hmm->M) <= RAMLIMIT)
(void) P7Viterbi(dsq[i], sqinfo[i].len, hmm, &(tr[i]));
else
(void) P7SmallViterbi(dsq[i], sqinfo[i].len, hmm, &(tr[i]));
}
/* Include an aligned alignment, if desired.
*/
if (mapali != NULL)
include_alignment(mapali, hmm, TRUE, &rseq, &dsq, &sqinfo, &tr, &nseq);
if (withali != NULL)
include_alignment(withali, hmm, FALSE, &rseq, &dsq, &sqinfo, &tr, &nseq);
/* Turn traces into a multiple alignment
*/
wgt = MallocOrDie(sizeof(float) * nseq);
FSet(wgt, nseq, 1.0);
P7Traces2Alignment(dsq, sqinfo, wgt, nseq, hmm->M, tr, matchonly,
&aseq, &ainfo);
/***********************************************
* Output the alignment
***********************************************/
if (outfile != NULL && (ofp = fopen(outfile, "w")) != NULL)
{
WriteSELEX(ofp, aseq, &ainfo, 50);
printf("Alignment saved in file %s\n", outfile);
fclose(ofp);
}
else
WriteSELEX(stdout, aseq, &ainfo, 50);
/***********************************************
* Cleanup and exit
***********************************************/
for (i = 0; i < nseq; i++)
{
P7FreeTrace(tr[i]);
FreeSequence(rseq[i], &(sqinfo[i]));
free(dsq[i]);
}
FreeAlignment(aseq, &ainfo);
FreePlan7(hmm);
free(sqinfo);
free(rseq);
free(dsq);
free(wgt);
free(tr);
SqdClean();
ajStrDel(&outfname);
ajStrDel(&infname);
ajStrDel(&ajseqfile);
#ifdef MEMDEBUG
current_size = malloc_inuse(&histid2);
if (current_size != orig_size) malloc_list(2, histid1, histid2);
else fprintf(stderr, "[No memory leaks.]\n");
#endif
ajSeqsetDel(&seqset);
ajFileClose(&ajwithali);
ajFileClose(&ajmapali);
embExit();
return 0;
}
