static int gt_splicesiteinfo_runner(int argc, const char **argv,
int parsed_args, void *tool_arguments,
GtError *err)
{
SpliceSiteInfoArguments *arguments = tool_arguments;
GtNodeStream *gff3_in_stream = NULL,
*add_introns_stream = NULL,
*splice_site_info_stream = NULL;
GtRegionMapping *region_mapping;
int had_err = 0;
gt_error_check(err);
gt_assert(arguments);
if (!had_err) {
/* create gff3 input stream */
gff3_in_stream = gt_gff3_in_stream_new_unsorted(argc - parsed_args,
argv + parsed_args);
/* create region mapping */
region_mapping = gt_seqid2file_region_mapping_new(arguments->s2fi, err);
if (!region_mapping)
had_err = -1;
}
if (!had_err) {
/* create addintrons stream (if necessary) */
if (arguments->addintrons)
add_introns_stream = gt_add_introns_stream_new(gff3_in_stream);
/* create extract feature stream */
splice_site_info_stream = gt_splice_site_info_stream_new(
arguments->addintrons
? add_introns_stream
: gff3_in_stream,
region_mapping);
/* pull the features through the stream and free them afterwards */
had_err = gt_node_stream_pull(splice_site_info_stream, err);
}
if (!had_err) {
if (!gt_splice_site_info_stream_show(splice_site_info_stream,
arguments->outfp)) {
gt_warning("input file(s) contained no intron, use option -addintrons to "
"add introns automatically");
}
}
/* free */
gt_node_stream_delete(splice_site_info_stream);
gt_node_stream_delete(add_introns_stream);
gt_node_stream_delete(gff3_in_stream);
return had_err;
}
static int gt_gff3_runner(int argc, const char **argv, int parsed_args,
void *tool_arguments, GtError *err)
{
GFF3Arguments *arguments = tool_arguments;
GtTypeChecker *type_checker = NULL;
GtNodeStream *gff3_in_stream,
*sort_stream = NULL,
*load_stream = NULL,
*merge_feature_stream = NULL,
*add_introns_stream = NULL,
*set_source_stream = NULL,
*gff3_out_stream = NULL,
*last_stream;
int had_err = 0;
gt_error_check(err);
gt_assert(arguments);
/* create a gff3 input stream */
gff3_in_stream = gt_gff3_in_stream_new_unsorted(argc - parsed_args,
argv + parsed_args);
if (arguments->verbose && arguments->outfp)
gt_gff3_in_stream_show_progress_bar((GtGFF3InStream*) gff3_in_stream);
if (arguments->checkids)
gt_gff3_in_stream_check_id_attributes((GtGFF3InStream*) gff3_in_stream);
if (!arguments->addids)
gt_gff3_in_stream_disable_add_ids(gff3_in_stream);
last_stream = gff3_in_stream;
/* set different type checker if necessary */
if (gt_typecheck_info_option_used(arguments->tci)) {
type_checker = gt_typecheck_info_create_type_checker(arguments->tci, err);
if (!type_checker)
had_err = -1;
if (!had_err)
gt_gff3_in_stream_set_type_checker(gff3_in_stream, type_checker);
}
/* set offset (if necessary) */
if (!had_err && arguments->offset != GT_UNDEF_WORD)
gt_gff3_in_stream_set_offset(gff3_in_stream, arguments->offset);
/* set offsetfile (if necessary) */
if (!had_err && gt_str_length(arguments->offsetfile)) {
had_err = gt_gff3_in_stream_set_offsetfile(gff3_in_stream,
arguments->offsetfile, err);
}
/* enable strict mode (if necessary) */
if (!had_err && arguments->strict)
gt_gff3_in_stream_enable_strict_mode((GtGFF3InStream*) gff3_in_stream);
/* enable tidy mode (if necessary) */
if (!had_err && arguments->tidy)
gt_gff3_in_stream_enable_tidy_mode((GtGFF3InStream*) gff3_in_stream);
if (!had_err && arguments->fixboundaries)
gt_gff3_in_stream_fix_region_boundaries((GtGFF3InStream*) gff3_in_stream);
/* create load stream (if necessary) */
if (!had_err && arguments->load) {
load_stream = gt_load_stream_new(last_stream);
last_stream = load_stream;
}
/* create sort stream (if necessary) */
if (!had_err && arguments->sort) {
sort_stream = gt_sort_stream_new(last_stream);
last_stream = sort_stream;
}
/* create merge feature stream (if necessary) */
if (!had_err && arguments->mergefeat) {
gt_assert(sort_stream);
merge_feature_stream = gt_merge_feature_stream_new(sort_stream);
last_stream = merge_feature_stream;
}
/* create addintrons stream (if necessary) */
if (!had_err && arguments->addintrons) {
gt_assert(last_stream);
add_introns_stream = gt_add_introns_stream_new(last_stream);
last_stream = add_introns_stream;
}
/* create setsource stream (if necessary) */
if (!had_err && gt_str_length(arguments->newsource) > 0) {
gt_assert(last_stream);
GtNodeVisitor *ssv = gt_set_source_visitor_new(arguments->newsource);
set_source_stream = gt_visitor_stream_new(last_stream, ssv);
last_stream = set_source_stream;
}
/* create gff3 output stream */
if (!had_err && arguments->show) {
gff3_out_stream = gt_gff3_out_stream_new(last_stream, arguments->outfp);
last_stream = gff3_out_stream;
gt_gff3_out_stream_set_fasta_width((GtGFF3OutStream*) last_stream,
arguments->width);
if (arguments->retainids)
gt_gff3_out_stream_retain_id_attributes((GtGFF3OutStream*) last_stream);
}
/* pull the features through the stream and free them afterwards */
if (!had_err)
had_err = gt_node_stream_pull(last_stream, err);
/* free */
gt_node_stream_delete(gff3_out_stream);
gt_node_stream_delete(sort_stream);
gt_node_stream_delete(load_stream);
gt_node_stream_delete(merge_feature_stream);
gt_node_stream_delete(add_introns_stream);
gt_node_stream_delete(set_source_stream);
gt_node_stream_delete(gff3_in_stream);
gt_type_checker_delete(type_checker);
return had_err;
}
static int gt_sketch_runner(int argc, const char **argv, int parsed_args,
void *tool_arguments, GT_UNUSED GtError *err)
{
GtSketchArguments *arguments = tool_arguments;
GtNodeStream *in_stream = NULL,
*add_introns_stream = NULL,
*gff3_out_stream = NULL,
*feature_stream = NULL,
*sort_stream = NULL,
*last_stream;
GtFeatureIndex *features = NULL;
const char *file;
char *seqid = NULL;
GtRange qry_range, sequence_region_range;
GtArray *results = NULL;
GtStyle *sty = NULL;
GtStr *prog, *defaultstylefile = NULL;
GtDiagram *d = NULL;
GtLayout *l = NULL;
GtImageInfo* ii = NULL;
GtCanvas *canvas = NULL;
GtUword height;
bool has_seqid;
int had_err = 0;
gt_error_check(err);
gt_assert(arguments);
prog = gt_str_new();
gt_str_append_cstr_nt(prog, argv[0],
gt_cstr_length_up_to_char(argv[0], ' '));
defaultstylefile = gt_get_gtdata_path(gt_str_get(prog), err);
gt_str_delete(prog);
if (!defaultstylefile)
had_err = -1;
if (!had_err) {
gt_str_append_cstr(defaultstylefile, "/sketch/default.style");
}
file = argv[parsed_args];
if (!had_err) {
/* create feature index */
features = gt_feature_index_memory_new();
parsed_args++;
/* create an input stream */
if (strcmp(gt_str_get(arguments->input), "gff") == 0)
{
in_stream = gt_gff3_in_stream_new_unsorted(argc - parsed_args,
argv + parsed_args);
if (arguments->verbose)
gt_gff3_in_stream_show_progress_bar((GtGFF3InStream*) in_stream);
} else if (strcmp(gt_str_get(arguments->input), "bed") == 0)
{
if (argc - parsed_args == 0)
in_stream = gt_bed_in_stream_new(NULL);
else
in_stream = gt_bed_in_stream_new(argv[parsed_args]);
} else if (strcmp(gt_str_get(arguments->input), "gtf") == 0)
{
if (argc - parsed_args == 0)
in_stream = gt_gtf_in_stream_new(NULL);
else
in_stream = gt_gtf_in_stream_new(argv[parsed_args]);
}
last_stream = in_stream;
/* create add introns stream if -addintrons was used */
if (arguments->addintrons) {
sort_stream = gt_sort_stream_new(last_stream);
add_introns_stream = gt_add_introns_stream_new(sort_stream);
last_stream = add_introns_stream;
}
/* create gff3 output stream if -pipe was used */
if (arguments->pipe) {
gff3_out_stream = gt_gff3_out_stream_new(last_stream, NULL);
last_stream = gff3_out_stream;
}
/* create feature stream */
feature_stream = gt_feature_stream_new(last_stream, features);
/* pull the features through the stream and free them afterwards */
had_err = gt_node_stream_pull(feature_stream, err);
gt_node_stream_delete(feature_stream);
gt_node_stream_delete(gff3_out_stream);
gt_node_stream_delete(sort_stream);
gt_node_stream_delete(add_introns_stream);
gt_node_stream_delete(in_stream);
}
if (!had_err) {
had_err = gt_feature_index_has_seqid(features,
&has_seqid,
gt_str_get(arguments->seqid),
err);
}
/* if seqid is empty, take first one added to index */
if (!had_err && strcmp(gt_str_get(arguments->seqid),"") == 0) {
seqid = gt_feature_index_get_first_seqid(features, err);
if (seqid == NULL) {
gt_error_set(err, "GFF input file must contain a sequence region!");
had_err = -1;
}
}
else if (!had_err && !has_seqid) {
gt_error_set(err, "sequence region '%s' does not exist in GFF input file",
gt_str_get(arguments->seqid));
had_err = -1;
}
else if (!had_err)
seqid = gt_str_get(arguments->seqid);
results = gt_array_new(sizeof (GtGenomeNode*));
if (!had_err) {
had_err = gt_feature_index_get_range_for_seqid(features,
&sequence_region_range,
seqid,
err);
}
if (!had_err) {
qry_range.start = (arguments->start == GT_UNDEF_UWORD ?
sequence_region_range.start :
arguments->start);
qry_range.end = (arguments->end == GT_UNDEF_UWORD ?
sequence_region_range.end :
arguments->end);
}
if (!had_err) {
if (arguments->verbose)
fprintf(stderr, "# of results: "GT_WU"\n", gt_array_size(results));
/* find and load style file */
if (!(sty = gt_style_new(err)))
had_err = -1;
if (gt_str_length(arguments->stylefile) == 0) {
gt_str_append_str(arguments->stylefile, defaultstylefile);
} else {
if (!had_err && gt_file_exists(gt_str_get(arguments->stylefile))) {
if (arguments->unsafe)
gt_style_unsafe_mode(sty);
}
else
{
had_err = -1;
gt_error_set(err, "style file '%s' does not exist!",
gt_str_get(arguments->stylefile));
}
}
if (!had_err)
had_err = gt_style_load_file(sty, gt_str_get(arguments->stylefile), err);
}
if (!had_err) {
/* create and write image file */
if (!(d = gt_diagram_new(features, seqid, &qry_range, sty, err)))
had_err = -1;
if (!had_err && arguments->flattenfiles)
gt_diagram_set_track_selector_func(d, flattened_file_track_selector,
NULL);
if (had_err || !(l = gt_layout_new(d, arguments->width, sty, err)))
had_err = -1;
if (!had_err)
had_err = gt_layout_get_height(l, &height, err);
if (!had_err) {
ii = gt_image_info_new();
if (strcmp(gt_str_get(arguments->format),"pdf")==0) {
canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_PDF,
arguments->width,
height, ii, err);
}
else if (strcmp(gt_str_get(arguments->format),"ps")==0) {
canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_PS,
arguments->width,
height, ii, err);
}
else if (strcmp(gt_str_get(arguments->format),"svg")==0) {
canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_SVG,
arguments->width,
height, ii, err);
}
else {
canvas = gt_canvas_cairo_file_new(sty, GT_GRAPHICS_PNG,
arguments->width,
height, ii, err);
}
if (!canvas)
had_err = -1;
if (!had_err) {
had_err = gt_layout_sketch(l, canvas, err);
}
if (!had_err) {
if (arguments->showrecmaps) {
GtUword i;
const GtRecMap *rm;
for (i = 0; i < gt_image_info_num_of_rec_maps(ii) ;i++) {
char buf[BUFSIZ];
rm = gt_image_info_get_rec_map(ii, i);
(void) gt_rec_map_format_html_imagemap_coords(rm, buf, BUFSIZ);
printf("%s, %s\n",
buf,
gt_feature_node_get_type(gt_rec_map_get_genome_feature(rm)));
}
}
if (arguments->use_streams) {
GtFile *outfile;
GtStr *str = gt_str_new();
gt_canvas_cairo_file_to_stream((GtCanvasCairoFile*) canvas, str);
outfile = gt_file_open(GT_FILE_MODE_UNCOMPRESSED, file, "w+", err);
if (outfile) {
gt_file_xwrite(outfile, gt_str_get_mem(str), gt_str_length(str));
gt_file_delete(outfile);
} else {
had_err = -1;
}
gt_str_delete(str);
} else {
had_err = gt_canvas_cairo_file_to_file((GtCanvasCairoFile*) canvas,
file,
err);
}
}
}
}
/* free */
gt_free(seqid);
gt_canvas_delete(canvas);
gt_layout_delete(l);
gt_image_info_delete(ii);
gt_style_delete(sty);
gt_diagram_delete(d);
gt_array_delete(results);
gt_str_delete(defaultstylefile);
gt_feature_index_delete(features);
return had_err;
}
