示例#1
0
void gt_linearalign_check_local(GT_UNUSED bool forward,
                                const GtUchar *useq, GtUword ulen,
                                const GtUchar *vseq, GtUword vlen)
{
  GtAlignment *align;
  GtWord score1, score2, score3, score4,
         matchscore = 2, mismatchscore = -2, gapscore = -1;
  GtLinspaceManagement *spacemanager;
  GtScoreHandler *scorehandler;

  if (memchr(useq, LINEAR_EDIST_GAP,ulen) != NULL)
  {
    fprintf(stderr,"%s: sequence u contains gap symbol\n",__func__);
    exit(GT_EXIT_PROGRAMMING_ERROR);
  }
  if (memchr(vseq, LINEAR_EDIST_GAP,vlen) != NULL)
  {
    fprintf(stderr,"%s: sequence v contains gap symbol\n",__func__);
    exit(GT_EXIT_PROGRAMMING_ERROR);
  }
  scorehandler = gt_scorehandler_new(matchscore, mismatchscore, 0, gapscore);
  gt_scorehandler_plain(scorehandler);
  spacemanager = gt_linspace_management_new();
  align = gt_alignment_new();
  score1 = gt_linearalign_compute_local_generic(spacemanager, scorehandler,
                                                align, useq, 0, ulen,
                                                vseq, 0, vlen);

  score2 = gt_alignment_eval_with_score(align, true, matchscore,
                                        mismatchscore, gapscore);
  gt_linspace_management_delete(spacemanager);
  gt_scorehandler_delete(scorehandler);
  if (score1 != score2)
  {
    fprintf(stderr,"gt_linearalign_compute_local_generic = "GT_WD" != "GT_WD
            " = gt_alignment_eval_generic_with_score\n", score1, score2);
    exit(GT_EXIT_PROGRAMMING_ERROR);
  }

  gt_alignment_reset(align);
  score3 = gt_squarealign_calculate_local(NULL, align, useq, 0, ulen,
                                          vseq, 0, vlen, matchscore,
                                          mismatchscore, gapscore);

  if (score1 != score3)
  {
    fprintf(stderr,"gt_linearalign_compute_local_generic = "GT_WD" != "GT_WD
            " = gt_squarealign_calculate_local\n", score1, score3);
    exit(GT_EXIT_PROGRAMMING_ERROR);
  }

  score4 = gt_alignment_eval_with_score(align, true, matchscore,
                                        mismatchscore, gapscore);
  if (score3 != score4)
  {
    fprintf(stderr,"gt_squarealign_calculate_local = "GT_WD" != "GT_WD
            " = gt_alignment_eval_generic_with_score\n", score3, score4);
    exit(GT_EXIT_PROGRAMMING_ERROR);
  }
  gt_alignment_delete(align);
}
示例#2
0
static int gt_linspace_align_runner(GT_UNUSED int argc,
                                    GT_UNUSED const char **argv,
                                    GT_UNUSED int parsed_args,
                                    void *tool_arguments,
                                    GtError *err)
{
  GtLinspaceArguments *arguments = tool_arguments;
  int had_err = 0;
  GtAlignment *align;
  GtWord left_dist = 0, right_dist = 0;
  GtSequenceTable *sequence_table1, *sequence_table2;
  GtLinspaceManagement *spacemanager;
  GtScoreHandler *scorehandler = NULL;
  GtTimer *linspacetimer = NULL;
  GtAlphabet *alphabet = NULL;

  gt_error_check(err);
  gt_assert(arguments);
  sequence_table1 = gt_sequence_table_new();
  sequence_table2 = gt_sequence_table_new();
  align = gt_alignment_new();
  spacemanager = gt_linspace_management_new();
  gt_linspace_management_set_TSfactor(spacemanager,arguments->timesquarefactor);

  /* get sequences */
  if (gt_str_array_size(arguments->strings) > 0)
  {
    get_onesequence(sequence_table1, arguments->strings, 0);
    sequence_table1->size++;
    get_onesequence(sequence_table2, arguments->strings, 1);
    sequence_table2->size++;
  }
  else if (gt_str_array_size(arguments->files) > 0)
  {
    had_err = get_fastasequences(sequence_table1,
                                 gt_str_array_get_str(arguments->files,0),err);
    if (!had_err)
    {
      had_err = get_fastasequences(sequence_table2,
                                  gt_str_array_get_str(arguments->files,1),err);
    }
  }
  if (arguments->dna)
  {
    alphabet = gt_alphabet_new_dna();
  } else
  {
    gt_assert(arguments->protein);
    alphabet = gt_alphabet_new_protein();
  }
  gt_encode_sequence_table(alphabet,sequence_table1);
  gt_encode_sequence_table(alphabet,sequence_table2);
  if (!had_err)
  {
    scorehandler = gt_arguments2scorehandler(arguments,err);
    if (scorehandler == NULL)
    {
      had_err = -1;
    } else
    {
      if (arguments->global && arguments->protein && !arguments->has_costmatrix)
      {
        GtScoreHandler *costhandler = gt_scorehandler2costhandler(scorehandler);
        gt_scorehandler_delete(scorehandler);
        scorehandler = costhandler;
      }
    }
  }
  /* get diagonal band */
  if (!had_err && arguments->diagonal)
  {
    if (gt_str_array_size(arguments->diagonalbonds) > 0)
    {
      had_err = gt_parse_score_value(__LINE__,&left_dist,
                                  gt_str_array_get(arguments->diagonalbonds,0),
                                  false, err);
      if (!had_err)
      {
        had_err = gt_parse_score_value(__LINE__,&right_dist,
                                  gt_str_array_get(arguments->diagonalbonds,1),
                                  false, err);
      }
    }
  }
  if (!had_err && arguments->spacetime)
  {
    linspacetimer = gt_timer_new();
  }

  /* alignment functions with linear gap costs */
  if (!had_err)
  {
    bool affine;

    if (gt_str_array_size(arguments->linearcosts) > 0)
    {
      affine = false;
    } else
    {
      gt_assert(gt_str_array_size(arguments->affinecosts) > 0);
      affine = true;
    }
    had_err = gt_all_against_all_alignment_check (
                            affine, align, arguments,
                            spacemanager,
                            scorehandler,
                            gt_alphabet_characters(alphabet),
                            gt_alphabet_wildcard_show(alphabet),
                            sequence_table1,
                            sequence_table2,
                            left_dist,
                            right_dist,
                            linspacetimer,err);
  }
  /*spacetime option*/
  if (!had_err && arguments->spacetime)
  {
    printf("# combined space peak in kilobytes: %f\n",
           GT_KILOBYTES(gt_linspace_management_get_spacepeak(spacemanager)));
    gt_timer_show_formatted(linspacetimer,"# TIME overall " GT_WD ".%02ld\n",
                            stdout);
  }
  gt_timer_delete(linspacetimer);
  gt_linspace_management_delete(spacemanager);
  gt_sequence_table_delete(sequence_table1);
  gt_sequence_table_delete(sequence_table2);
  gt_alignment_delete(align);
  gt_alphabet_delete(alphabet);
  gt_scorehandler_delete(scorehandler);
  return had_err;
}
示例#3
0
void gt_linearalign_check(GT_UNUSED bool forward,
                          const GtUchar *useq,
                          GtUword ulen,
                          const GtUchar *vseq,
                          GtUword vlen)
{
  GtAlignment *align;
  GtUword edist1, edist2, edist3, edist4,
          matchcost = 0, mismatchcost = 1, gapcost = 1;
  GtLinspaceManagement *spacemanager;
  GtScoreHandler *scorehandler;
  const bool downcase = true;

  if (memchr(useq, LINEAR_EDIST_GAP,ulen) != NULL)
  {
    fprintf(stderr,"%s: sequence u contains gap symbol\n",__func__);
    exit(GT_EXIT_PROGRAMMING_ERROR);
  }
  if (memchr(vseq, LINEAR_EDIST_GAP,vlen) != NULL)
  {
    fprintf(stderr,"%s: sequence v contains gap symbol\n",__func__);
    exit(GT_EXIT_PROGRAMMING_ERROR);
  }

  scorehandler = gt_scorehandler_new(matchcost,  mismatchcost, 0, gapcost);
  gt_scorehandler_plain(scorehandler);
  gt_scorehandler_downcase(scorehandler);
  spacemanager = gt_linspace_management_new();
  align = gt_alignment_new_with_seqs(useq, ulen, vseq, vlen);
  edist1 = gt_calc_linearalign(spacemanager, scorehandler, align,
                               useq, 0, ulen,
                               vseq, 0, vlen);
  edist2 = gt_squarealign_global_distance_only(useq, 0, ulen, vseq, 0, vlen,
                                               scorehandler);

  if (edist1 != edist2)
  {
    fprintf(stderr,"gt_calc_linearalign = "GT_WU" != "GT_WU
            " = gt_squarealign_global_distance_only\n", edist1,edist2);
    exit(GT_EXIT_PROGRAMMING_ERROR);
  }

  edist3 = gt_alignment_eval_with_score(align, true, matchcost,
                                        mismatchcost, gapcost);

  if (edist2 != edist3)
  {
    fprintf(stderr,"gt_squarealign_global_distance_only = "GT_WU" != "GT_WU
            " = gt_alignment_eval_with_score\n", edist2,edist3);
    exit(GT_EXIT_PROGRAMMING_ERROR);
  }

  edist4 = gt_calc_linearedist(downcase,useq, ulen, vseq, vlen);
  if (edist3 != edist4)
  {
    fprintf(stderr,"gt_alignment_eval_with_score = "GT_WU" != "GT_WU
            " = gt_calc_linearedist\n", edist3, edist4);
    exit(GT_EXIT_PROGRAMMING_ERROR);
  }
  gt_linspace_management_delete(spacemanager);
  gt_scorehandler_delete(scorehandler);
  gt_alignment_delete(align);
}