/******************************************************************************
* This function attempts to parse genomic coordinates from the
* current sequence header. If successful it will set the sequence
* name to the chromosome name, and set the starting sequence position.
*
* Returns TRUE if it was able to find genomic coordinates, FALSE otherwise.
*****************************************************************************/
static BOOLEAN_T parse_genomic_coordinates(
SEQ_READER_FROM_FASTA_T *fasta_reader
) {
// Copy chromosome name and position to reader
int end_pos;
return (
parse_genomic_coordinates_helper(
fasta_reader->sequence_header,
&(fasta_reader->sequence_name),
&(fasta_reader->sequence_name_len),
&(fasta_reader->current_position),
&end_pos
)
);
}
/**************************************************************************
* Dump sequence matches sorted by the name of the sequence.
*
* Outputs Columns:
* 1) Trimmed lowercase sequence with uppercase matches.
* 2) Position of the secondary match within the whole sequence.
* 3) Sequence fragment that the primary matched.
* 4) Strand of the primary match (+|-)
* 5) Sequence fragment that the secondary matched.
* 6) Strand of the secondary match (+|-)
* 7) Is the primary match on the same strand as the secondary (s|o)
* 8) Is the secondary match downstream or upstream (d|u)
* 9) The gap between the primary and secondary matches
* 10) The name of the sequence
* 11) The p-value of the bin containing the match (adjusted for # of bins)
* ---if the FASTA input file sequence names are in Genome Browser format:
* 12-14) Position of primary match in BED coordinates
* 15) Position of primary match in Genome Browser coordinates
* 16-18) Position of secondary match in BED coordinates
* 19) Position of secondary match in Genome Browser coordinates
*
* If you wish to sort based on the gap column:
* Sort individual output:
* sort -n -k 9,9 -o seqs_primary_secondary.txt seqs_primary_secondary.txt
* Or sort all outputs:
* for f in seqs_*.txt; do sort -n -k 9,9 -o $f $f; done
* Or to get just locations of primary motif in BED coordinates
* where the secondary is on the opposite strand, upstream with a gap of 118bp:
* awk '$7=="o" && $8=="u" && $9==118 {print $12"\t"$13"\t"$14;}' seqs_primary_secondary.txt
*
**************************************************************************/
static void dump_sequence_matches(FILE *out, int margin, int bin,
double sigthresh, BOOLEAN_T sig_only, RBTREE_T *sequences,
MOTIF_T *primary_motif, SECONDARY_MOTIF_T *secondary_motif,
ARRAY_T **matches) {
RBNODE_T *node;
SEQUENCE_T *sequence;
int idx, seqlen, i, j, start, end, secondary, secondary_pos, primary_len, secondary_len, distance;
BOOLEAN_T primary_rc, secondary_rc, downstream;
char *buffer, *seq, *primary_match, *secondary_match;
ARRAY_T *secondary_array;
ALPH_T *alph;
// get the alphabet
alph = get_motif_alph(primary_motif);
// allocate a buffer for copying the trimmed sequence into and modify it
seqlen = margin * 2 + get_motif_trimmed_length(primary_motif);
buffer = (char*)mm_malloc(sizeof(char) * (seqlen + 1));
// get the lengths of the motifs
primary_len = get_motif_trimmed_length(primary_motif);
secondary_len = get_motif_trimmed_length(secondary_motif->motif);
// allocate some strings for storing the matches
primary_match = (char*)mm_malloc(sizeof(char) * (primary_len + 1));
secondary_match = (char*)mm_malloc(sizeof(char) * (secondary_len + 1));
// add null byte at the end of the match strings
primary_match[primary_len] = '\0';
secondary_match[secondary_len] = '\0';
// iterate over all the sequences
for (node = rbtree_first(sequences); node != NULL; node = rbtree_next(node)) {
sequence = (SEQUENCE_T*)rbtree_value(node);
primary_rc = get_array_item(0, sequence->primary_matches) < 0;
//secondary = matches[sequence->index];
secondary_array = matches[sequence->index];
if (! secondary_array) continue;
int n_secondary_matches = get_array_length(secondary_array);
for (idx=0; idx<n_secondary_matches; idx++) {
secondary = get_array_item(idx, secondary_array);
secondary_rc = secondary < 0;
secondary_pos = abs(secondary);
// calculate the distance
if (secondary_pos <= margin) {
distance = margin - secondary_pos - secondary_len + 1;
downstream = primary_rc;
} else {
distance = secondary_pos - margin - primary_len - 1;
downstream = !primary_rc;
}
// copy the trimmed sequence
seq = sequence->data;
for (i = 0; i < seqlen; ++i) {
buffer[i] = (alph_is_case_insensitive(alph) ? tolower(seq[i]) : seq[i]);
}
buffer[seqlen] = '\0';
// uppercase primary
start = margin;
end = margin + primary_len;
for (i = start, j = 0; i < end; ++i, ++j) {
buffer[i] = (alph_is_case_insensitive(alph) ? toupper(buffer[i]) : buffer[i]);
primary_match[j] = buffer[i];
}
// uppercase secondary
// note orign was one, subtract 1 to make origin zero as required for arrays
start = secondary_pos -1;
end = start + secondary_len;
for (i = start, j = 0; i < end; ++i, ++j) {
buffer[i] = (alph_is_case_insensitive(alph) ? toupper(buffer[i]) : buffer[i]);
secondary_match[j] = buffer[i];
}
// get the p-value of the seconndary match
SPACING_T *spacings;
if (secondary_rc == primary_rc) {
spacings = downstream ? secondary_motif->spacings+(SAME+RIGHT) : secondary_motif->spacings+(SAME+LEFT);
} else {
spacings = downstream ? secondary_motif->spacings+(OPPO+RIGHT) : secondary_motif->spacings+(OPPO+LEFT);
}
double p_value = spacings->pvalue[distance/bin];
// skip match if not significant and only reporting significant matches
if (sig_only && (p_value > sigthresh)) continue;
// output line to file
fprintf(out, "%s %3d %s %s %s %s %s %s %3d %s %.1e",
buffer,
secondary_pos,
primary_match,
(primary_rc ? "-" : "+"),
secondary_match,
(secondary_rc ? "-" : "+"),
(secondary_rc == primary_rc ? "s" : "o"),
(downstream ? "d" : "u"),
distance,
sequence->name,
p_value
);
// Parse the sequence name to see if we can get genomic coordinates
// and print additional columns with primary and secondary matches
// in both BED and Genome Browser coordinates.
char *chr_name;
size_t chr_name_len;
int start_pos, end_pos;
if (parse_genomic_coordinates_helper(
sequence->name,
&chr_name,
&chr_name_len,
&start_pos,
&end_pos))
{
// Get the start and end of the primary match in
// 0-relative, half-open genomic coordinates.
int p_start = start_pos + fabs(get_array_item(0, sequence->primary_matches)) - 1;
int p_end = p_start + primary_len;
// Get the start and end of the secondary match in
// 0-relative, half-open genomic coordinates.
int s_start, s_end;
if ( (!primary_rc && downstream) || (primary_rc && !downstream) ) {
s_start = p_end + distance;
s_end = s_start + secondary_len;
} else {
s_end = p_start - distance;
s_start = s_end - secondary_len;
}
fprintf(out, " %s %d %d %s:%d-%d",
chr_name, p_start, p_end, chr_name, p_start+1, p_end);
fprintf(out, " %s %d %d %s:%d-%d\n",
chr_name, s_start, s_end, chr_name, s_start+1, s_end);
} else {
fprintf(out, "\n");
}
} // secondary match
} // primary match
free(buffer);
free(primary_match);
free(secondary_match);
}
