int bamfixmatecoordinates(::libmaus::util::ArgInfo const & arginfo)
{
::libmaus::util::TempFileRemovalContainer::setup();
::libmaus::timing::RealTimeClock rtc; rtc.start();
bool const verbose = arginfo.getValue<unsigned int>("verbose",getDefaultVerbose());
unsigned int const colhashbits = arginfo.getValue<unsigned int>("colhashbits",getDefaultColHashBits());
unsigned int const collistsize = arginfo.getValue<unsigned int>("collistsize",getDefaultColListSize());
int const level = arginfo.getValue<int>("level",getDefaultLevel());
std::string const tmpfilenamebase = arginfo.getValue<std::string>("tmpfile",arginfo.getDefaultTmpFileName());
switch ( level )
{
case Z_NO_COMPRESSION:
case Z_BEST_SPEED:
case Z_BEST_COMPRESSION:
case Z_DEFAULT_COMPRESSION:
break;
default:
{
::libmaus::exception::LibMausException se;
se.getStream()
<< "Unknown compression level, please use"
<< " level=" << Z_DEFAULT_COMPRESSION << " (default) or"
<< " level=" << Z_BEST_SPEED << " (fast) or"
<< " level=" << Z_BEST_COMPRESSION << " (best) or"
<< " level=" << Z_NO_COMPRESSION << " (no compression)" << std::endl;
se.finish();
throw se;
}
break;
}
std::string const tmpfilename = tmpfilenamebase + "_bamcollate";
::libmaus::util::TempFileRemovalContainer::addTempFile(tmpfilename);
::libmaus::bambam::CollatingBamDecoder CBD(std::cin,tmpfilename,false /* put rank */,colhashbits/*hash bits*/,collistsize/*size of output list*/);
::libmaus::bambam::BamFormatAuxiliary auxdata;
::libmaus::bambam::BamHeader const & bamheader = CBD.getHeader();
// add PG line to header
std::string const upheadtext = ::libmaus::bambam::ProgramHeaderLineSet::addProgramLine(
bamheader.text,
"bamfixmatecoordinates", // ID
"bamfixmatecoordinates", // PN
arginfo.commandline, // CL
::libmaus::bambam::ProgramHeaderLineSet(bamheader.text).getLastIdInChain(), // PP
std::string(PACKAGE_VERSION) // VN
);
// construct new header
::libmaus::bambam::BamHeader uphead(upheadtext);
if ( uphead.getSortOrder() != "queryname" )
uphead.changeSortOrder("unknown");
/*
* start index/md5 callbacks
*/
std::string const tmpfileindex = tmpfilenamebase + "_index";
::libmaus::util::TempFileRemovalContainer::addTempFile(tmpfileindex);
std::string md5filename;
std::string indexfilename;
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > cbs;
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Pmd5cb;
if ( arginfo.getValue<unsigned int>("md5",getDefaultMD5()) )
{
if ( arginfo.hasArg("md5filename") && arginfo.getUnparsedValue("md5filename","") != "" )
md5filename = arginfo.getUnparsedValue("md5filename","");
else
std::cerr << "[V] no filename for md5 given, not creating hash" << std::endl;
if ( md5filename.size() )
{
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Tmd5cb(new ::libmaus::lz::BgzfDeflateOutputCallbackMD5);
Pmd5cb = UNIQUE_PTR_MOVE(Tmd5cb);
cbs.push_back(Pmd5cb.get());
}
}
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Pindex;
if ( arginfo.getValue<unsigned int>("index",getDefaultIndex()) )
{
if ( arginfo.hasArg("indexfilename") && arginfo.getUnparsedValue("indexfilename","") != "" )
indexfilename = arginfo.getUnparsedValue("indexfilename","");
else
std::cerr << "[V] no filename for index given, not creating index" << std::endl;
if ( indexfilename.size() )
{
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Tindex(new libmaus::bambam::BgzfDeflateOutputCallbackBamIndex(tmpfileindex));
Pindex = UNIQUE_PTR_MOVE(Tindex);
cbs.push_back(Pindex.get());
}
}
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > * Pcbs = 0;
if ( cbs.size() )
Pcbs = &cbs;
/*
* end md5/index callbacks
*/
// setup bam writer
::libmaus::bambam::BamWriter::unique_ptr_type writer(new ::libmaus::bambam::BamWriter(std::cout,uphead,level,Pcbs));
#if 0
::libmaus::bambam::ProgramHeaderLineSet PHLS(bamheader.text);
std::cerr << "Last id in PG chain: " << PHLS.getLastIdInChain() << std::endl;
#endif
// std::cout << bamheader.text;
typedef ::libmaus::bambam::CollatingBamDecoder::alignment_ptr_type alignment_ptr_type;
std::pair<alignment_ptr_type,alignment_ptr_type> P;
uint64_t const mod = 1024*1024;
uint64_t proc = 0;
uint64_t lastproc = 0;
uint64_t paircnt = 0;
while ( CBD.tryPair(P) )
{
uint64_t const mapcnt = getMapCnt(P.first) + getMapCnt(P.second);
if ( mapcnt == 1 )
{
int32_t refid = -1;
int32_t pos = -1;
if ( P.first )
{
refid = P.first->getRefID();
pos = P.first->getPos();
}
else
{
assert ( P.second );
refid = P.second->getRefID();
pos = P.second->getPos();
}
P.first->putRefId(refid);
P.first->putPos(pos);
P.first->putNextRefId(refid);
P.first->putNextPos(pos);
P.second->putRefId(refid);
P.second->putPos(pos);
P.second->putNextRefId(refid);
P.second->putNextPos(pos);
}
if ( P.first )
{
P.first->serialise(writer->getStream());
++proc;
}
if ( P.second )
{
P.second->serialise(writer->getStream());
++proc;
}
if ( P.first && P.second )
{
paircnt++;
}
if ( verbose && (proc/mod != lastproc/mod) )
{
std::cerr
<< "Processed " << proc << " fragments, " << paircnt << " pairs, "
<< proc/rtc.getElapsedSeconds() << " al/s"
<< std::endl;
lastproc = proc;
}
}
if ( verbose )
std::cerr
<< "Processed " << proc << " fragments, " << paircnt << " pairs, "
<< proc/rtc.getElapsedSeconds() << " al/s"
<< std::endl;
writer.reset();
if ( Pmd5cb )
{
Pmd5cb->saveDigestAsFile(md5filename);
}
if ( Pindex )
{
Pindex->flush(std::string(indexfilename));
}
return EXIT_SUCCESS;
}
int bamheap2(libmaus::util::ArgInfo const & arginfo)
{
bool const verbose = arginfo.getValue("verbose",getDefaultVerbose());
std::string const reference = arginfo.getUnparsedValue("reference",std::string());
std::string const outputprefix = arginfo.getUnparsedValue("outputprefix",std::string());
libmaus::bambam::BamAlignmentDecoderWrapper::unique_ptr_type decwrapper(
libmaus::bambam::BamMultiAlignmentDecoderFactory::construct(arginfo));
::libmaus::bambam::BamAlignmentDecoder * ppdec = &(decwrapper->getDecoder());
::libmaus::bambam::BamAlignmentDecoder & dec = *ppdec;
::libmaus::bambam::BamHeader const & header = dec.getHeader();
::libmaus::bambam::BamAlignment const & algn = dec.getAlignment();
double const damult = arginfo.getValue<double>("amult",1);
double const dcmult = arginfo.getValue<double>("cmult",1);
double const dgmult = arginfo.getValue<double>("gmult",1);
double const dtmult = arginfo.getValue<double>("tmult",1);
double const dpadmult = arginfo.getValue<double>("padmult",1);
double maxmult = 0;
maxmult = std::max(damult,maxmult);
maxmult = std::max(dcmult,maxmult);
maxmult = std::max(dgmult,maxmult);
maxmult = std::max(dtmult,maxmult);
maxmult = std::max(dpadmult,maxmult);
uint64_t const amult = std::floor((damult / maxmult) * (1ull<<16) + 0.5);
uint64_t const cmult = std::floor((dcmult / maxmult) * (1ull<<16) + 0.5);
uint64_t const gmult = std::floor((dgmult / maxmult) * (1ull<<16) + 0.5);
uint64_t const tmult = std::floor((dtmult / maxmult) * (1ull<<16) + 0.5);
uint64_t const padmult = std::floor((dpadmult / maxmult) * (1ull<<16) + 0.5);
libmaus::fastx::FastAIndex::unique_ptr_type Pindex;
libmaus::aio::CheckedInputStream::unique_ptr_type PCIS;
if ( reference.size() )
{
libmaus::fastx::FastAIndex::unique_ptr_type Tindex(
libmaus::fastx::FastAIndex::load(reference+".fai")
);
Pindex = UNIQUE_PTR_MOVE(Tindex);
libmaus::aio::CheckedInputStream::unique_ptr_type TCIS(new libmaus::aio::CheckedInputStream(reference));
PCIS = UNIQUE_PTR_MOVE(TCIS);
}
libmaus::autoarray::AutoArray<libmaus::bambam::cigar_operation> cigop;
libmaus::autoarray::AutoArray<char> bases;
int64_t prevrefid = -1;
std::string refidname = "*";
std::map< uint64_t, HeapEntry > M;
uint64_t alcnt = 0;
std::vector< std::pair<char,uint8_t> > pendinginserts;
int64_t loadedRefId = -1;
int64_t streamRefId = -1;
libmaus::autoarray::AutoArray<char> refseqbases;
ConsensusAccuracy * consacc = 0;
std::map<uint64_t,ConsensusAccuracy> Mconsacc;
typedef libmaus::util::shared_ptr<std::ostringstream>::type stream_ptr_type;
stream_ptr_type Pstream;
ConsensusAux Caux;
Caux.M['a'] = Caux.M['A'] = amult;
Caux.M['c'] = Caux.M['C'] = cmult;
Caux.M['g'] = Caux.M['G'] = gmult;
Caux.M['t'] = Caux.M['T'] = tmult;
Caux.M[padsym] = padmult;
while ( dec.readAlignment() )
{
if ( algn.isMapped() && (!algn.isQCFail()) )
{
assert ( ! pendinginserts.size() );
uint32_t const numcigop = algn.getCigarOperations(cigop);
uint64_t readpos = 0;
uint64_t refpos = algn.getPos();
uint64_t const seqlen = algn.decodeRead(bases);
uint8_t const * qual = libmaus::bambam::BamAlignmentDecoderBase::getQual(algn.D.begin());
// handle finished columns
if ( algn.getRefID() != prevrefid )
{
while ( M.size() )
{
HeapEntry & H = M.begin()->second;
if ( outputprefix.size() && (streamRefId != prevrefid) )
{
if ( Pstream )
{
std::ostringstream fnostr;
fnostr << outputprefix << "_" << header.getRefIDName(streamRefId);
libmaus::aio::PosixFdOutputStream PFOS(fnostr.str());
PFOS << ">" << header.getRefIDName(streamRefId) << '\n';
PFOS << Pstream->str() << '\n';
Pstream.reset();
}
stream_ptr_type Tstream(new std::ostringstream);
Pstream = Tstream;
streamRefId = prevrefid;
}
if ( Pindex && (loadedRefId != prevrefid) )
{
refseqbases = Pindex->readSequence(*PCIS, Pindex->getSequenceIdByName(refidname));
loadedRefId = prevrefid;
if ( Mconsacc.find(loadedRefId) == Mconsacc.end() )
Mconsacc[loadedRefId] = ConsensusAccuracy(refseqbases.size());
consacc = &(Mconsacc[loadedRefId]);
}
H.toStream(std::cout,M.begin()->first,refidname,(M.begin()->first < refseqbases.size()) ? static_cast<int>(refseqbases[M.begin()->first]) : -1,Caux,consacc,Pstream.get());
M.erase(M.begin());
}
prevrefid = algn.getRefID();
refidname = header.getRefIDName(prevrefid);
}
else
{
while ( M.size() && M.begin()->first < refpos )
{
HeapEntry & H = M.begin()->second;
if ( outputprefix.size() && (streamRefId != prevrefid) )
{
if ( Pstream )
{
std::ostringstream fnostr;
fnostr << outputprefix << "_" << header.getRefIDName(streamRefId);
libmaus::aio::PosixFdOutputStream PFOS(fnostr.str());
PFOS << ">" << header.getRefIDName(streamRefId) << '\n';
PFOS << Pstream->str() << '\n';
Pstream.reset();
}
stream_ptr_type Tstream(new std::ostringstream);
Pstream = Tstream;
streamRefId = prevrefid;
}
if ( Pindex && (loadedRefId != prevrefid) )
{
refseqbases = Pindex->readSequence(*PCIS, Pindex->getSequenceIdByName(refidname));
loadedRefId = prevrefid;
if ( Mconsacc.find(loadedRefId) == Mconsacc.end() )
Mconsacc[loadedRefId] = ConsensusAccuracy(refseqbases.size());
consacc = &(Mconsacc[loadedRefId]);
}
H.toStream(std::cout,M.begin()->first,refidname,(M.begin()->first < refseqbases.size()) ? static_cast<int>(refseqbases[M.begin()->first]) : -1,Caux,consacc,Pstream.get());
M.erase(M.begin());
}
}
for ( uint64_t ci = 0; ci < numcigop; ++ci )
{
uint64_t const ciglen = cigop[ci].second;
switch ( cigop[ci].first )
{
case libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_CMATCH:
case libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_CEQUAL:
case libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_CDIFF:
{
if ( pendinginserts.size() )
{
M[refpos].I.push_back(pendinginserts);
pendinginserts.resize(0);
}
for ( uint64_t i = 0; i < ciglen; ++i )
{
M[refpos].V.push_back(std::make_pair(bases[readpos],qual[readpos]));
readpos++;
refpos++;
}
break;
}
case libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_CINS:
{
for ( uint64_t i = 0; i < ciglen; ++i, ++readpos )
pendinginserts.push_back(std::make_pair(bases[readpos],qual[readpos]));
break;
}
case libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_CDEL:
// handle pending inserts
if ( pendinginserts.size() )
{
M[refpos].I.push_back(pendinginserts);
pendinginserts.resize(0);
}
// deleting bases from the reference
for ( uint64_t i = 0; i < ciglen; ++i, ++refpos )
M[refpos].V.push_back(std::make_pair(padsym,0));
break;
case libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_CREF_SKIP:
// handle pending inserts
if ( pendinginserts.size() )
{
M[refpos].I.push_back(pendinginserts);
pendinginserts.resize(0);
}
// skip bases on reference
for ( uint64_t i = 0; i < ciglen; ++i )
{
refpos++;
}
break;
case libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_CSOFT_CLIP:
// skip bases on read
for ( uint64_t i = 0; i < ciglen; ++i )
{
readpos++;
}
break;
case libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_CHARD_CLIP:
break;
case libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_CPAD:
{
for ( uint64_t i = 0; i < ciglen; ++i, ++readpos )
pendinginserts.push_back(std::make_pair(padsym,0));
break;
}
}
}
if ( pendinginserts.size() )
{
M[refpos].I.push_back(pendinginserts);
M[refpos].iadd++;
pendinginserts.resize(0);
}
assert ( readpos == seqlen );
}
if ( verbose && ((++alcnt % (1024*1024)) == 0) )
std::cerr << "[V] " << alcnt << std::endl;
}
while ( M.size() )
{
HeapEntry & H = M.begin()->second;
if ( outputprefix.size() && (streamRefId != prevrefid) )
{
if ( Pstream )
{
std::ostringstream fnostr;
fnostr << outputprefix << "_" << header.getRefIDName(streamRefId);
libmaus::aio::PosixFdOutputStream PFOS(fnostr.str());
PFOS << ">" << header.getRefIDName(streamRefId) << '\n';
PFOS << Pstream->str() << '\n';
Pstream.reset();
}
stream_ptr_type Tstream(new std::ostringstream);
Pstream = Tstream;
streamRefId = prevrefid;
}
if ( Pindex && (loadedRefId != prevrefid) )
{
refseqbases = Pindex->readSequence(*PCIS, Pindex->getSequenceIdByName(refidname));
loadedRefId = prevrefid;
if ( Mconsacc.find(loadedRefId) == Mconsacc.end() )
Mconsacc[loadedRefId] = ConsensusAccuracy(refseqbases.size());
consacc = &(Mconsacc[loadedRefId]);
}
H.toStream(std::cout,M.begin()->first,refidname,(M.begin()->first < refseqbases.size()) ? static_cast<int>(refseqbases[M.begin()->first]) : -1,Caux,consacc,Pstream.get());
M.erase(M.begin());
}
if ( Pstream )
{
std::ostringstream fnostr;
fnostr << outputprefix << "_" << header.getRefIDName(streamRefId);
libmaus::aio::PosixFdOutputStream PFOS(fnostr.str());
PFOS << ">" << header.getRefIDName(streamRefId) << '\n';
PFOS << Pstream->str() << '\n';
Pstream.reset();
}
ConsensusAccuracy constotal;
for ( std::map<uint64_t,ConsensusAccuracy>::const_iterator ita = Mconsacc.begin(); ita != Mconsacc.end(); ++ita )
{
std::cerr << header.getRefIDName(ita->first) << "\t" << ita->second << std::endl;
std::map<uint64_t,uint64_t> const M = ita->second.depthhistogram.get();
uint64_t total = 0;
uint64_t preavg = 0;
for ( std::map<uint64_t,uint64_t>::const_iterator aita = M.begin(); aita != M.end(); ++aita )
{
total += aita->second;
preavg += aita->first * aita->second;
}
uint64_t acc = 0;
for ( std::map<uint64_t,uint64_t>::const_iterator aita = M.begin(); aita != M.end(); ++aita )
{
acc += aita->second;
std::cerr << "H[" << header.getRefIDName(ita->first) << "," << aita->first << ",+]"
<< "\t" << aita->second << "\t" << static_cast<double>(aita->second)/total
<< "\t" << acc << "\t" << static_cast<double>(acc)/total << std::endl;
}
acc = 0;
for ( std::map<uint64_t,uint64_t>::const_reverse_iterator aita = M.rbegin(); aita != M.rend(); ++aita )
{
acc += aita->second;
std::cerr << "H[" << header.getRefIDName(ita->first) << "," << aita->first << ",-]"
<< "\t" << aita->second << "\t" << static_cast<double>(aita->second)/total
<< "\t" << acc << "\t" << static_cast<double>(acc)/total << std::endl;
}
std::cerr << "H[" << header.getRefIDName(ita->first) << ",avg]\t" <<
static_cast<double>(preavg)/total << std::endl;
constotal += ita->second;
}
if ( Mconsacc.size() )
{
std::cerr << "all\t" << constotal << std::endl;
std::map<uint64_t,uint64_t> const M = constotal.depthhistogram.get();
uint64_t total = 0;
uint64_t preavg = 0;
for ( std::map<uint64_t,uint64_t>::const_iterator aita = M.begin(); aita != M.end(); ++aita )
{
total += aita->second;
preavg += aita->first * aita->second;
}
uint64_t acc = 0;
for ( std::map<uint64_t,uint64_t>::const_iterator aita = M.begin(); aita != M.end(); ++aita )
{
acc += aita->second;
std::cerr << "H[" << "all" << "," << aita->first << ",+]"
<< "\t" << aita->second << "\t" << static_cast<double>(aita->second)/total
<< "\t" << acc << "\t" << static_cast<double>(acc)/total << std::endl;
}
acc = 0;
for ( std::map<uint64_t,uint64_t>::const_reverse_iterator aita = M.rbegin(); aita != M.rend(); ++aita )
{
acc += aita->second;
std::cerr << "H[" << "all" << "," << aita->first << ",-]"
<< "\t" << aita->second << "\t" << static_cast<double>(aita->second)/total
<< "\t" << acc << "\t" << static_cast<double>(acc)/total << std::endl;
}
std::cerr << "H[all,avg]\t" << static_cast<double>(preavg) / total << std::endl;
}
return EXIT_SUCCESS;
}
void normalisefastaBgzf(libmaus::util::ArgInfo const & arginfo, std::ostream & out)
{
libmaus::fastx::StreamFastAReaderWrapper in(std::cin);
libmaus::fastx::StreamFastAReaderWrapper::pattern_type pattern;
int const level = libmaus::bambam::BamBlockWriterBaseFactory::checkCompressionLevel(arginfo.getValue("level",getDefaultLevel()));
std::string const indexfn = arginfo.getUnparsedValue("index","");
::libmaus::bambam::BamBlockWriterBaseFactory::checkCompressionLevel(level);
libmaus::lz::BgzfDeflate<std::ostream> defl(out,level,false /* full flush */);
uint64_t const inbufsize = defl.getInputBufferSize();
uint64_t zoffset = 0;
uint64_t ioffset = 0;
std::vector<libmaus::fastx::BgzfFastAIndexEntry> index;
std::ostringstream indexstr;
ioffset += libmaus::util::NumberSerialisation::serialiseNumber(indexstr,inbufsize);
uint64_t patid = 0;
while ( in.getNextPatternUnlocked(pattern) )
{
std::string const name = pattern.getStringId();
std::string const shortname = stripName(name);
std::string const & spat = pattern.spattern;
char const * cpat = spat.c_str();
uint64_t const patlen = spat.size();
uint64_t const numblocks = (patlen + inbufsize - 1)/inbufsize;
index.push_back(libmaus::fastx::BgzfFastAIndexEntry(shortname,patid++,ioffset));
ioffset += libmaus::util::StringSerialisation::serialiseString(indexstr,name);
ioffset += libmaus::util::StringSerialisation::serialiseString(indexstr,shortname);
ioffset += libmaus::util::NumberSerialisation::serialiseNumber(indexstr,patlen);
ioffset += libmaus::util::NumberSerialisation::serialiseNumber(indexstr,zoffset);
ioffset += libmaus::util::NumberSerialisation::serialiseNumber(indexstr,numblocks);
std::ostringstream nameostr;
nameostr << '>' << name << '\n';
std::string const nameser = nameostr.str();
std::pair<uint64_t,uint64_t> const P0 = defl.writeSyncedCount(nameser.c_str(),nameser.size());
zoffset += P0.second;
uint64_t o = 0;
while ( o != patlen )
{
assert ( o % inbufsize == 0 );
uint64_t const towrite = std::min(patlen-o,inbufsize);
std::pair<uint64_t,uint64_t> const P1 = defl.writeSyncedCount(cpat,towrite);
ioffset += libmaus::util::NumberSerialisation::serialiseNumber(indexstr,zoffset);
zoffset += P1.second;
o += towrite;
cpat += towrite;
}
ioffset += libmaus::util::NumberSerialisation::serialiseNumber(indexstr,zoffset);
std::pair<uint64_t,uint64_t> const Pn = defl.writeSyncedCount("\n",1);
zoffset += Pn.second;
}
defl.flush();
out << std::flush;
uint64_t const imetaoffset = ioffset;
ioffset += libmaus::util::NumberSerialisation::serialiseNumber(indexstr,index.size());
for ( uint64_t i = 0; i < index.size(); ++i )
ioffset += libmaus::util::NumberSerialisation::serialiseNumber(indexstr,index[i].ioffset);
libmaus::util::NumberSerialisation::serialiseNumber(indexstr,imetaoffset);
if ( indexfn.size() )
{
std::string const & sindex = indexstr.str();
libmaus::aio::CheckedOutputStream indexCOS(indexfn);
indexCOS.write(sindex.c_str(),sindex.size());
indexCOS.flush();
indexCOS.close();
}
}
static void filterBamUsedSequences(
libmaus::util::ArgInfo const & arginfo,
std::istream & in,
::libmaus::bitio::IndexedBitVector const & IBV,
std::ostream & out
)
{
libmaus::lz::BgzfInflateStream bgzfin(in);
libmaus::bambam::BamHeaderLowMem::unique_ptr_type PBHLM ( libmaus::bambam::BamHeaderLowMem::constructFromBAM(bgzfin));
bool const verbose = arginfo.getValue<unsigned int>("verbose",getDefaultVerbose());
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > cbs;
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Pmd5cb;
std::string md5filename;
if ( arginfo.getValue<unsigned int>("md5",getDefaultMD5()) )
{
if ( arginfo.hasArg("md5filename") && arginfo.getUnparsedValue("md5filename","") != "" )
md5filename = arginfo.getUnparsedValue("md5filename","");
else
std::cerr << "[V] no filename for md5 given, not creating hash" << std::endl;
if ( md5filename.size() )
{
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Tmd5cb(new ::libmaus::lz::BgzfDeflateOutputCallbackMD5);
Pmd5cb = UNIQUE_PTR_MOVE(Tmd5cb);
cbs.push_back(Pmd5cb.get());
}
}
int const level = libmaus::bambam::BamBlockWriterBaseFactory::checkCompressionLevel(arginfo.getValue<int>("level",getDefaultLevel()));
libmaus::lz::BgzfDeflate<std::ostream>::unique_ptr_type Pbgzfout(
new libmaus::lz::BgzfDeflate<std::ostream>(
out,level
)
);
libmaus::lz::BgzfDeflate<std::ostream> & bgzfout = *Pbgzfout;
if ( verbose )
std::cerr << "[V] writing filtered header...";
PBHLM->serialiseSequenceSubset(bgzfout,IBV,"bamfilterheader2" /* id */,"bamfilterheader2" /* pn */,
arginfo.commandline /* pgCL */, PACKAGE_VERSION /* pgVN */
);
if ( verbose )
std::cerr << "done." << std::endl;
::libmaus::bambam::BamAlignment algn;
uint64_t c = 0;
while ( libmaus::bambam::BamAlignmentDecoder::readAlignmentGz(bgzfin,algn) )
{
if ( algn.isMapped() )
{
int64_t const refid = algn.getRefID();
assert ( refid >= 0 );
assert ( IBV.get(refid) );
algn.putRefId(IBV.rank1(refid)-1);
}
else
{
algn.putRefId(-1);
}
if ( algn.isPaired() && algn.isMapped() )
{
int64_t const refid = algn.getNextRefID();
assert ( refid >= 0 );
assert ( IBV.get(refid) );
algn.putNextRefId(IBV.rank1(refid)-1);
}
else
{
algn.putNextRefId(-1);
}
algn.serialise(bgzfout);
if ( verbose && ( ((++c) & (1024*1024-1)) == 0 ) )
std::cerr << "[V] " << c/(1024*1024) << std::endl;
}
bgzfout.flush();
bgzfout.addEOFBlock();
Pbgzfout.reset();
if ( Pmd5cb )
Pmd5cb->saveDigestAsFile(md5filename);
}
int bamfilter(libmaus::util::ArgInfo const & arginfo)
{
uint64_t const minmapped = arginfo.getValue<uint64_t>("minmapped",getDefaultMinMapped());
uint64_t const maxmapped = arginfo.getValue<uint64_t>("maxmapped",getDefaultMaxMapped());
uint64_t const minlen = arginfo.getValue<uint64_t>("minlen",getDefaultMinLen());
int const level = libmaus::bambam::BamBlockWriterBaseFactory::checkCompressionLevel(arginfo.getValue<int>("level",getDefaultLevel()));
::libmaus::bambam::BamDecoder BD(std::cin);
::libmaus::bambam::BamHeader const & bamheader = BD.getHeader();
::libmaus::bambam::BamAlignment & alignment = BD.getAlignment();
/*
* start index/md5 callbacks
*/
std::string const tmpfilenamebase = arginfo.getValue<std::string>("tmpfile",arginfo.getDefaultTmpFileName());
std::string const tmpfileindex = tmpfilenamebase + "_index";
::libmaus::util::TempFileRemovalContainer::addTempFile(tmpfileindex);
std::string md5filename;
std::string indexfilename;
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > cbs;
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Pmd5cb;
if ( arginfo.getValue<unsigned int>("md5",getDefaultMD5()) )
{
if ( arginfo.hasArg("md5filename") && arginfo.getUnparsedValue("md5filename","") != "" )
md5filename = arginfo.getUnparsedValue("md5filename","");
else
std::cerr << "[V] no filename for md5 given, not creating hash" << std::endl;
if ( md5filename.size() )
{
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Tmd5cb(new ::libmaus::lz::BgzfDeflateOutputCallbackMD5);
Pmd5cb = UNIQUE_PTR_MOVE(Tmd5cb);
cbs.push_back(Pmd5cb.get());
}
}
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Pindex;
if ( arginfo.getValue<unsigned int>("index",getDefaultIndex()) )
{
if ( arginfo.hasArg("indexfilename") && arginfo.getUnparsedValue("indexfilename","") != "" )
indexfilename = arginfo.getUnparsedValue("indexfilename","");
else
std::cerr << "[V] no filename for index given, not creating index" << std::endl;
if ( indexfilename.size() )
{
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Tindex(new libmaus::bambam::BgzfDeflateOutputCallbackBamIndex(tmpfileindex));
Pindex = UNIQUE_PTR_MOVE(Tindex);
cbs.push_back(Pindex.get());
}
}
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > * Pcbs = 0;
if ( cbs.size() )
Pcbs = &cbs;
/*
* end md5/index callbacks
*/
::libmaus::bambam::BamHeader::unique_ptr_type uphead(libmaus::bambam::BamHeaderUpdate::updateHeader(arginfo,bamheader,"bamfilter",std::string(PACKAGE_VERSION)));
::libmaus::bambam::BamWriter::unique_ptr_type writer(new ::libmaus::bambam::BamWriter(std::cout,*uphead,level,Pcbs));
while ( BD.readAlignment() )
{
bool const a_1_mapped = !(alignment.getFlags() & ::libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_FUNMAP);
bool const a_2_mapped = !(alignment.getFlags() & ::libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_FMUNMAP);
bool const proper = (alignment.getFlags() & ::libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_FPROPER_PAIR);
uint64_t const nummapped = (a_1_mapped?1:0)+(a_2_mapped?1:0)+(proper?1:0);
if (
nummapped >= minmapped &&
nummapped <= maxmapped &&
alignment.getLseq() >= static_cast<int64_t>(minlen)
)
alignment.serialise(writer->getStream());
}
writer.reset();
if ( Pmd5cb )
{
Pmd5cb->saveDigestAsFile(md5filename);
}
if ( Pindex )
{
Pindex->flush(std::string(indexfilename));
}
return EXIT_SUCCESS;
}
int bamsplitmod(libmaus::util::ArgInfo const & arginfo)
{
if ( isatty(STDIN_FILENO) )
{
::libmaus::exception::LibMausException se;
se.getStream() << "Refusing read binary data from terminal, please redirect standard input to pipe or file." << std::endl;
se.finish();
throw se;
}
int const level = libmaus::bambam::BamBlockWriterBaseFactory::checkCompressionLevel(arginfo.getValue<int>("level",getDefaultLevel()));
int const verbose = arginfo.getValue<int>("verbose",getDefaultVerbose());
uint64_t const div = arginfo.getValue<int>("div",getDefaultDiv());
std::string const prefix = arginfo.getUnparsedValue("prefix",getDefaultFilePrefix(arginfo));
if ( ! div )
{
::libmaus::exception::LibMausException se;
se.getStream() << "div cannot be 0." << std::endl;
se.finish();
throw se;
}
libmaus::bambam::BamDecoder bamdec(std::cin);
libmaus::bambam::BamAlignment const & algn = bamdec.getAlignment();
libmaus::bambam::BamHeader const & header = bamdec.getHeader();
::libmaus::bambam::BamHeader::unique_ptr_type uphead(updateHeader(arginfo,header));
libmaus::autoarray::AutoArray<libmaus::aio::CheckedOutputStream::unique_ptr_type> COS(div);
libmaus::autoarray::AutoArray<libmaus::bambam::BamWriter::unique_ptr_type> writers(div);
std::vector < std::string > filenames;
for ( uint64_t i = 0; i < div; ++i )
{
std::ostringstream ostr;
ostr << prefix << "_" << std::setw(6) << std::setfill('0') << i << std::setw(0) << ".bam";
libmaus::aio::CheckedOutputStream::unique_ptr_type tCOS(new libmaus::aio::CheckedOutputStream(ostr.str()));
COS[i] = UNIQUE_PTR_MOVE(tCOS);
libmaus::bambam::BamWriter::unique_ptr_type twriter(new libmaus::bambam::BamWriter(*COS[i],*uphead,level));
writers[i] = UNIQUE_PTR_MOVE(twriter);
}
uint64_t c = 0;
if ( verbose )
{
while ( bamdec.readAlignment() )
{
algn.serialise ( writers [ (c++) % div ] -> getStream() );
if ( ((c) & ((1ull<<20)-1)) == 0 )
std::cerr << "[V] " << c << std::endl;
}
std::cerr << "[V] " << c << std::endl;
}
else
{
while ( bamdec.readAlignment() )
algn.serialise ( writers [ (c++) % div ] -> getStream() );
}
for ( uint64_t i = 0; i < div; ++i )
{
writers[i].reset();
COS[i]->flush();
COS[i].reset();
}
return EXIT_SUCCESS;
}
int bamfixmatecoordinatesnamesorted(::libmaus::util::ArgInfo const & arginfo)
{
bool const verbose = arginfo.getValue<unsigned int>("verbose",getDefaultVerbose());
::libmaus::timing::RealTimeClock rtc; rtc.start();
// gzip compression level for output
int const level = libmaus::bambam::BamBlockWriterBaseFactory::checkCompressionLevel(arginfo.getValue<int>("level",getDefaultLevel()));
::libmaus::bambam::BamDecoder bamfile(std::cin);
std::string const headertext(bamfile.getHeader().text);
// add PG line to header
std::string const upheadtext = ::libmaus::bambam::ProgramHeaderLineSet::addProgramLine(
headertext,
"bamfixmatecoordinatesnamesorted", // ID
"bamfixmatecoordinatesnamesorted", // PN
arginfo.commandline, // CL
::libmaus::bambam::ProgramHeaderLineSet(headertext).getLastIdInChain(), // PP
std::string(PACKAGE_VERSION) // VN
);
// construct new header
::libmaus::bambam::BamHeader uphead(upheadtext);
if ( uphead.getSortOrder() != "queryname" )
uphead.changeSortOrder("unknown");
std::string const & finalheadtext = uphead.text;
::libmaus::bambam::BamHeader finalheader(finalheadtext);
/*
* start index/md5 callbacks
*/
std::string const tmpfilenamebase = arginfo.getValue<std::string>("tmpfile",arginfo.getDefaultTmpFileName());
std::string const tmpfileindex = tmpfilenamebase + "_index";
::libmaus::util::TempFileRemovalContainer::addTempFile(tmpfileindex);
std::string md5filename;
std::string indexfilename;
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > cbs;
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Pmd5cb;
if ( arginfo.getValue<unsigned int>("md5",getDefaultMD5()) )
{
if ( arginfo.hasArg("md5filename") && arginfo.getUnparsedValue("md5filename","") != "" )
md5filename = arginfo.getUnparsedValue("md5filename","");
else
std::cerr << "[V] no filename for md5 given, not creating hash" << std::endl;
if ( md5filename.size() )
{
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Tmd5cb(new ::libmaus::lz::BgzfDeflateOutputCallbackMD5);
Pmd5cb = UNIQUE_PTR_MOVE(Tmd5cb);
cbs.push_back(Pmd5cb.get());
}
}
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Pindex;
if ( arginfo.getValue<unsigned int>("index",getDefaultIndex()) )
{
if ( arginfo.hasArg("indexfilename") && arginfo.getUnparsedValue("indexfilename","") != "" )
indexfilename = arginfo.getUnparsedValue("indexfilename","");
else
std::cerr << "[V] no filename for index given, not creating index" << std::endl;
if ( indexfilename.size() )
{
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Tindex(new libmaus::bambam::BgzfDeflateOutputCallbackBamIndex(tmpfileindex));
Pindex = UNIQUE_PTR_MOVE(Tindex);
cbs.push_back(Pindex.get());
}
}
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > * Pcbs = 0;
if ( cbs.size() )
Pcbs = &cbs;
/*
* end md5/index callbacks
*/
::libmaus::bambam::BamWriter::unique_ptr_type writer(new ::libmaus::bambam::BamWriter(std::cout,finalheader,level,Pcbs));
std::pair< std::pair< ::libmaus::bambam::BamAlignment::shared_ptr_type, bool> , std::pair< ::libmaus::bambam::BamAlignment::shared_ptr_type, bool> >
P(std::pair< ::libmaus::bambam::BamAlignment::shared_ptr_type, bool>(::libmaus::bambam::BamAlignment::shared_ptr_type(),false),std::pair< ::libmaus::bambam::BamAlignment::shared_ptr_type, bool>(::libmaus::bambam::BamAlignment::shared_ptr_type(),false));
// try to read two alignments
P.first.second = bamfile.readAlignment();
if ( P.first.second )
{
P.first.first = bamfile.salignment();
P.second.second = P.first.second && bamfile.readAlignment();
P.second.first = bamfile.salignment();
}
uint64_t single = 0, pairs = 0;
uint64_t proc = 0;
uint64_t lastproc = 0;
uint64_t const mod = 1024*1024;
// while we have two alignments
while ( P.first.second && P.second.second )
{
uint32_t const aflags = P.first.first->getFlags();
uint32_t const bflags = P.second.first->getFlags();
// same name?
if (
(aflags & ::libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_FPAIRED)
&&
(bflags & ::libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_FPAIRED)
&&
(! strcmp(P.first.first->getName(),P.second.first->getName()))
)
{
unsigned int const amap = (aflags & ::libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_FUNMAP) ? 0 : 1;
unsigned int const bmap = (bflags & ::libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_FUNMAP) ? 0 : 1;
// std::cerr << "Pair " << bam1_qname(P.first.first->alignment) << " amap=" << amap << " bmap=" << bmap << std::endl;
// if exactly one of the two is mapped
if ( amap + bmap == 1 )
{
::libmaus::bambam::BamAlignment::shared_ptr_type mapped = amap ? P.first.first : P.second.first;
int64_t const tid = mapped->getRefID();
int64_t const pos = mapped->getPos();
// std::cerr << "tid=" << tid << " pos=" << pos << std::endl;
// set all tid and pos values
P.first.first->putRefId(tid);
P.first.first->putPos(pos);
P.first.first->putNextRefId(tid);
P.first.first->putNextPos(pos);
P.second.first->putRefId(tid);
P.second.first->putPos(pos);
P.second.first->putNextRefId(tid);
P.second.first->putNextPos(pos);
}
// write alignments
P.first.first->serialise(writer->getStream());
P.second.first->serialise(writer->getStream());
// read new alignments
P.first.second = bamfile.readAlignment();
if ( P.first.second )
{
P.first.first = bamfile.salignment();
P.second.second = bamfile.readAlignment();
P.second.first = bamfile.salignment();
}
pairs++;
proc += 2;
}
// different names
else
{
// write first alignment
P.first.first->serialise(writer->getStream());
// move second to first
std::swap(P.first,P.second);
// read new second
P.second.second = P.first.second && bamfile.readAlignment();
if ( P.second.second )
P.second.first = bamfile.salignment();
single++;
proc += 1;
}
if ( verbose && (proc/mod != lastproc/mod) )
{
std::cerr << proc << "\t" << single << "\t" << pairs << "\t" <<
proc/rtc.getElapsedSeconds() << "al/s"
<< std::endl;
lastproc = proc;
}
}
if ( P.first.second )
{
P.first.first->serialise(writer->getStream());
single++;
proc += 1;
}
if ( verbose )
std::cerr << proc << "\t" << single << "\t" << pairs << "\t" <<
proc/rtc.getElapsedSeconds() << "al/s"
<< std::endl;
assert ( ! P.second.second );
writer.reset();
if ( Pmd5cb )
{
Pmd5cb->saveDigestAsFile(md5filename);
}
if ( Pindex )
{
Pindex->flush(std::string(indexfilename));
}
return EXIT_SUCCESS;
}
int bamcat(libmaus::util::ArgInfo const & arginfo)
{
if ( isatty(STDOUT_FILENO) )
{
::libmaus::exception::LibMausException se;
se.getStream() << "Refusing write binary data to terminal, please redirect standard output to pipe or file." << std::endl;
se.finish();
throw se;
}
int const level = arginfo.getValue<int>("level",getDefaultLevel());
int const verbose = arginfo.getValue<int>("verbose",getDefaultVerbose());
switch ( level )
{
case Z_NO_COMPRESSION:
case Z_BEST_SPEED:
case Z_BEST_COMPRESSION:
case Z_DEFAULT_COMPRESSION:
break;
default:
{
::libmaus::exception::LibMausException se;
se.getStream()
<< "Unknown compression level, please use"
<< " level=" << Z_DEFAULT_COMPRESSION << " (default) or"
<< " level=" << Z_BEST_SPEED << " (fast) or"
<< " level=" << Z_BEST_COMPRESSION << " (best) or"
<< " level=" << Z_NO_COMPRESSION << " (no compression)" << std::endl;
se.finish();
throw se;
}
break;
}
std::vector<std::string> inputfilenames = arginfo.getPairValues("I");
for ( uint64_t i = 0; i < arginfo.restargs.size(); ++i )
inputfilenames.push_back(arginfo.restargs[i]);
libmaus::bambam::BamCat bamdec(inputfilenames /* ,true */);
libmaus::bambam::BamAlignment const & algn = bamdec.getAlignment();
libmaus::bambam::BamHeader const & header = bamdec.getHeader();
::libmaus::bambam::BamHeader::unique_ptr_type uphead(updateHeader(arginfo,header));
/*
* start index/md5 callbacks
*/
std::string const tmpfilenamebase = arginfo.getValue<std::string>("tmpfile",arginfo.getDefaultTmpFileName());
std::string const tmpfileindex = tmpfilenamebase + "_index";
::libmaus::util::TempFileRemovalContainer::addTempFile(tmpfileindex);
std::string md5filename;
std::string indexfilename;
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > cbs;
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Pmd5cb;
if ( arginfo.getValue<unsigned int>("md5",getDefaultMD5()) )
{
if ( arginfo.hasArg("md5filename") && arginfo.getUnparsedValue("md5filename","") != "" )
md5filename = arginfo.getUnparsedValue("md5filename","");
else
std::cerr << "[V] no filename for md5 given, not creating hash" << std::endl;
if ( md5filename.size() )
{
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Tmd5cb(new ::libmaus::lz::BgzfDeflateOutputCallbackMD5);
Pmd5cb = UNIQUE_PTR_MOVE(Tmd5cb);
cbs.push_back(Pmd5cb.get());
}
}
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Pindex;
if ( arginfo.getValue<unsigned int>("index",getDefaultIndex()) )
{
if ( arginfo.hasArg("indexfilename") && arginfo.getUnparsedValue("indexfilename","") != "" )
indexfilename = arginfo.getUnparsedValue("indexfilename","");
else
std::cerr << "[V] no filename for index given, not creating index" << std::endl;
if ( indexfilename.size() )
{
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Tindex(new libmaus::bambam::BgzfDeflateOutputCallbackBamIndex(tmpfileindex));
Pindex = UNIQUE_PTR_MOVE(Tindex);
cbs.push_back(Pindex.get());
}
}
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > * Pcbs = 0;
if ( cbs.size() )
Pcbs = &cbs;
/*
* end md5/index callbacks
*/
::libmaus::bambam::BamWriter::unique_ptr_type writer(new ::libmaus::bambam::BamWriter(std::cout,*uphead,level,Pcbs));
libmaus::bambam::BamWriter::stream_type & bamoutstr = writer->getStream();
if ( verbose )
{
uint64_t c = 0;
while ( bamdec.readAlignment() )
{
algn.serialise(bamoutstr);
if ( ((++c) & ((1ull<<20)-1)) == 0 )
std::cerr << "[V] " << c << std::endl;
}
std::cerr << "[V] " << c << std::endl;
}
else
while ( bamdec.readAlignment() )
algn.serialise(bamoutstr);
writer.reset();
if ( Pmd5cb )
{
Pmd5cb->saveDigestAsFile(md5filename);
}
if ( Pindex )
{
Pindex->flush(std::string(indexfilename));
}
return EXIT_SUCCESS;
}
int bam12split(::libmaus::util::ArgInfo const & arginfo)
{
::libmaus::util::TempFileRemovalContainer::setup();
if ( isatty(STDIN_FILENO) )
{
::libmaus::exception::LibMausException se;
se.getStream() << "Refusing to read binary data from terminal, please redirect standard input to pipe or file." << std::endl;
se.finish();
throw se;
}
if ( isatty(STDOUT_FILENO) )
{
::libmaus::exception::LibMausException se;
se.getStream() << "Refusing write binary data to terminal, please redirect standard output to pipe or file." << std::endl;
se.finish();
throw se;
}
if ( arginfo.hasArg("keep") && arginfo.hasArg("remove") )
{
::libmaus::exception::LibMausException se;
se.getStream() << "The keep and remove keys are mutually exclusive." << std::endl;
se.finish();
throw se;
}
int const level = libmaus::bambam::BamBlockWriterBaseFactory::checkCompressionLevel(arginfo.getValue<int>("level",getDefaultLevel()));
int const verbose = arginfo.getValue<int>("verbose",getDefaultVerbose());
::libmaus::bambam::BamDecoder dec(std::cin,false);
::libmaus::bambam::BamHeader const & header = dec.getHeader();
std::string const headertext(header.text);
// add PG line to header
std::string const upheadtext = ::libmaus::bambam::ProgramHeaderLineSet::addProgramLine(
headertext,
"bam12split", // ID
"bam12split", // PN
arginfo.commandline, // CL
::libmaus::bambam::ProgramHeaderLineSet(headertext).getLastIdInChain(), // PP
std::string(PACKAGE_VERSION) // VN
);
// construct new header
libmaus::bambam::BamHeader uphead(upheadtext);
uphead.changeSortOrder("unknown");
/*
* start index/md5 callbacks
*/
std::string const tmpfilenamebase = arginfo.getValue<std::string>("tmpfile",arginfo.getDefaultTmpFileName());
std::string const tmpfileindex = tmpfilenamebase + "_index";
::libmaus::util::TempFileRemovalContainer::addTempFile(tmpfileindex);
std::string md5filename;
std::string indexfilename;
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > cbs;
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Pmd5cb;
if ( arginfo.getValue<unsigned int>("md5",getDefaultMD5()) )
{
if ( arginfo.hasArg("md5filename") && arginfo.getUnparsedValue("md5filename","") != "" )
md5filename = arginfo.getUnparsedValue("md5filename","");
else
std::cerr << "[V] no filename for md5 given, not creating hash" << std::endl;
if ( md5filename.size() )
{
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Tmd5cb(new ::libmaus::lz::BgzfDeflateOutputCallbackMD5);
Pmd5cb = UNIQUE_PTR_MOVE(Tmd5cb);
cbs.push_back(Pmd5cb.get());
}
}
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Pindex;
if ( arginfo.getValue<unsigned int>("index",getDefaultIndex()) )
{
if ( arginfo.hasArg("indexfilename") && arginfo.getUnparsedValue("indexfilename","") != "" )
indexfilename = arginfo.getUnparsedValue("indexfilename","");
else
std::cerr << "[V] no filename for index given, not creating index" << std::endl;
if ( indexfilename.size() )
{
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Tindex(new libmaus::bambam::BgzfDeflateOutputCallbackBamIndex(tmpfileindex));
Pindex = UNIQUE_PTR_MOVE(Tindex);
cbs.push_back(Pindex.get());
}
}
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > * Pcbs = 0;
if ( cbs.size() )
Pcbs = &cbs;
/*
* end md5/index callbacks
*/
::libmaus::bambam::BamWriter::unique_ptr_type writer(new ::libmaus::bambam::BamWriter(std::cout,uphead,level,Pcbs));
libmaus::bambam::BamAlignment & algn = dec.getAlignment();
uint64_t c = 0;
while ( dec.readAlignment() )
{
bool const ok = split12(algn);
if ( ok )
algn.serialise(writer->getStream());
if ( verbose && (++c & (1024*1024-1)) == 0 )
std::cerr << "[V] " << c/(1024*1024) << std::endl;
}
writer.reset();
if ( Pmd5cb )
{
Pmd5cb->saveDigestAsFile(md5filename);
}
if ( Pindex )
{
Pindex->flush(std::string(indexfilename));
}
return EXIT_SUCCESS;
}
int bamclipreinsert(::libmaus::util::ArgInfo const & arginfo)
{
if ( isatty(STDIN_FILENO) )
{
::libmaus::exception::LibMausException se;
se.getStream() << "Refusing to read binary data from terminal, please redirect standard input to pipe or file." << std::endl;
se.finish();
throw se;
}
if ( isatty(STDOUT_FILENO) )
{
::libmaus::exception::LibMausException se;
se.getStream() << "Refusing write binary data to terminal, please redirect standard output to pipe or file." << std::endl;
se.finish();
throw se;
}
int const level = arginfo.getValue<int>("level",getDefaultLevel());
int const verbose = arginfo.getValue<int>("verbose",getDefaultVerbose());
switch ( level )
{
case Z_NO_COMPRESSION:
case Z_BEST_SPEED:
case Z_BEST_COMPRESSION:
case Z_DEFAULT_COMPRESSION:
break;
default:
{
::libmaus::exception::LibMausException se;
se.getStream()
<< "Unknown compression level, please use"
<< " level=" << Z_DEFAULT_COMPRESSION << " (default) or"
<< " level=" << Z_BEST_SPEED << " (fast) or"
<< " level=" << Z_BEST_COMPRESSION << " (best) or"
<< " level=" << Z_NO_COMPRESSION << " (no compression)" << std::endl;
se.finish();
throw se;
}
break;
}
::libmaus::bambam::BamDecoder dec(std::cin,false);
::libmaus::bambam::BamHeader const & header = dec.getHeader();
std::string const headertext(header.text);
// add PG line to header
std::string const upheadtext = ::libmaus::bambam::ProgramHeaderLineSet::addProgramLine(
headertext,
"bamclipreinsert", // ID
"bamclipreinsert", // PN
arginfo.commandline, // CL
::libmaus::bambam::ProgramHeaderLineSet(headertext).getLastIdInChain(), // PP
std::string(PACKAGE_VERSION) // VN
);
// construct new header
libmaus::bambam::BamHeader const uphead(upheadtext);
/*
* start index/md5 callbacks
*/
std::string const tmpfilenamebase = arginfo.getValue<std::string>("tmpfile",arginfo.getDefaultTmpFileName());
std::string const tmpfileindex = tmpfilenamebase + "_index";
::libmaus::util::TempFileRemovalContainer::addTempFile(tmpfileindex);
std::string md5filename;
std::string indexfilename;
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > cbs;
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Pmd5cb;
if ( arginfo.getValue<unsigned int>("md5",getDefaultMD5()) )
{
if ( arginfo.hasArg("md5filename") && arginfo.getUnparsedValue("md5filename","") != "" )
md5filename = arginfo.getUnparsedValue("md5filename","");
else
std::cerr << "[V] no filename for md5 given, not creating hash" << std::endl;
if ( md5filename.size() )
{
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Tmd5cb(new ::libmaus::lz::BgzfDeflateOutputCallbackMD5);
Pmd5cb = UNIQUE_PTR_MOVE(Tmd5cb);
cbs.push_back(Pmd5cb.get());
}
}
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Pindex;
if ( arginfo.getValue<unsigned int>("index",getDefaultIndex()) )
{
if ( arginfo.hasArg("indexfilename") && arginfo.getUnparsedValue("indexfilename","") != "" )
indexfilename = arginfo.getUnparsedValue("indexfilename","");
else
std::cerr << "[V] no filename for index given, not creating index" << std::endl;
if ( indexfilename.size() )
{
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Tindex(new libmaus::bambam::BgzfDeflateOutputCallbackBamIndex(tmpfileindex));
Pindex = UNIQUE_PTR_MOVE(Tindex);
cbs.push_back(Pindex.get());
}
}
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > * Pcbs = 0;
if ( cbs.size() )
Pcbs = &cbs;
/*
* end md5/index callbacks
*/
::libmaus::bambam::BamWriter::unique_ptr_type writer(new ::libmaus::bambam::BamWriter(std::cout,uphead,level,Pcbs));
libmaus::bambam::BamAuxFilterVector bafv;
// bafv.set('z','z');
// std::vector<uint8_t> R(8);
// std::string const zz("zz");
libmaus::bambam::BamAlignment & algn = dec.getAlignment();
uint64_t c = 0;
libmaus::autoarray::AutoArray < std::pair<uint8_t,uint8_t> > auxtags;
libmaus::autoarray::AutoArray<libmaus::bambam::cigar_operation> cigop;
std::stack < libmaus::bambam::cigar_operation > hardstack;
libmaus::bambam::BamAlignment::D_array_type Tcigar;
while ( dec.readAlignment() )
{
// reinsert clipped parts and attach soft clipping cigar operations as needed
clipReinsert(algn,auxtags,bafv,cigop,Tcigar,hardstack);
algn.serialise(writer->getStream());
++c;
if ( verbose && (c & (1024*1024-1)) == 0 )
std::cerr << "[V] " << c/(1024*1024) << std::endl;
}
writer.reset();
if ( Pmd5cb )
{
Pmd5cb->saveDigestAsFile(md5filename);
}
if ( Pindex )
{
Pindex->flush(std::string(indexfilename));
}
return EXIT_SUCCESS;
}
int bam12auxmerge(::libmaus::util::ArgInfo const & arginfo)
{
if ( isatty(STDIN_FILENO) )
{
::libmaus::exception::LibMausException se;
se.getStream() << "Refusing to read binary data from terminal, please redirect standard input to pipe or file." << std::endl;
se.finish();
throw se;
}
if ( isatty(STDOUT_FILENO) )
{
::libmaus::exception::LibMausException se;
se.getStream() << "Refusing write binary data to terminal, please redirect standard output to pipe or file." << std::endl;
se.finish();
throw se;
}
std::string const prefilename = arginfo.getRestArg<std::string>(0);
libmaus::bambam::BamDecoder bampredec(prefilename);
int const level = libmaus::bambam::BamBlockWriterBaseFactory::checkCompressionLevel(arginfo.getValue<int>("level",getDefaultLevel()));
int const verbose = arginfo.getValue<int>("verbose",getDefaultVerbose());
int const ranksplit = arginfo.getValue<int>("ranksplit",getDefaultRankSplit());
int const rankstrip = arginfo.getValue<int>("rankstrip",getDefaultRankSplit());
int const clipreinsert = arginfo.getValue<int>("clipreinsert",getDefaultClipReinsert());
int const zztoname = arginfo.getValue<int>("zztoname",getDefaultZZToName());
uint64_t const mod = arginfo.getValue<int>("mod",getDefaultMod());
uint64_t const bmod = libmaus::math::nextTwoPow(mod);
uint64_t const bmask = bmod-1;
libmaus::autoarray::AutoArray<char> Aread;
::libmaus::bambam::BamDecoder bamdec(std::cin,false);
::libmaus::bambam::BamHeader const & header = bamdec.getHeader();
::libmaus::bambam::BamHeader const & preheader = bampredec.getHeader();
std::string const headertext(header.text);
std::string const preheadertext(libmaus::bambam::HeaderLine::removeSequenceLines(preheader.text));
libmaus::bambam::ProgramHeaderLineSet headerlines(headertext);
libmaus::bambam::ProgramHeaderLineSet preheaderlines(preheadertext);
std::vector<libmaus::bambam::HeaderLine> allheaderlines = libmaus::bambam::HeaderLine::extractLines(headertext);
std::string const lastid = preheaderlines.getLastIdInChain();
std::stack < std::pair<uint64_t,std::string> > pgtodo;
for ( uint64_t i = 0; i < headerlines.roots.size(); ++i )
pgtodo.push(std::pair<uint64_t,std::string>(headerlines.roots[i],lastid));
std::string upheadtext = preheadertext;
while ( pgtodo.size() )
{
uint64_t const hid = pgtodo.top().first;
std::string const PP = pgtodo.top().second;
pgtodo.pop();
libmaus::bambam::HeaderLine const & line = headerlines.lines[hid];
// ID, PP, PN, CL, VN
std::string ID = (line.M.find("ID") != line.M.end()) ? line.M.find("ID")->second : "";
std::string const PN = (line.M.find("PN") != line.M.end()) ? line.M.find("PN")->second : "";
std::string const CL = (line.M.find("CL") != line.M.end()) ? line.M.find("CL")->second : "";
std::string const VN = (line.M.find("VN") != line.M.end()) ? line.M.find("VN")->second : "";
upheadtext = ::libmaus::bambam::ProgramHeaderLineSet::addProgramLineRef(
upheadtext,
ID,
PN,
CL,
PP,
VN
);
if ( headerlines.edges.find(hid) != headerlines.edges.end() )
{
std::vector<uint64_t> const & children = headerlines.edges.find(hid)->second;
for ( uint64_t j = 0; j < children.size(); ++j )
pgtodo.push(std::pair<uint64_t,std::string>(children[j],ID));
}
}
/* copy SQ lines */
std::ostringstream sqconcstr;
sqconcstr << upheadtext;
for ( uint64_t i = 0; i < allheaderlines.size(); ++i )
if ( allheaderlines[i].type == "SQ" )
sqconcstr << allheaderlines[i].line << "\n";
upheadtext = sqconcstr.str();
::libmaus::bambam::BamHeader uphead(upheadtext);
uphead.changeSortOrder("unknown");
/*
* start index/md5 callbacks
*/
std::string const tmpfilenamebase = arginfo.getValue<std::string>("tmpfile",arginfo.getDefaultTmpFileName());
std::string const tmpfileindex = tmpfilenamebase + "_index";
::libmaus::util::TempFileRemovalContainer::addTempFile(tmpfileindex);
std::string md5filename;
std::string indexfilename;
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > cbs;
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Pmd5cb;
if ( arginfo.getValue<unsigned int>("md5",getDefaultMD5()) )
{
if ( arginfo.hasArg("md5filename") && arginfo.getUnparsedValue("md5filename","") != "" )
md5filename = arginfo.getUnparsedValue("md5filename","");
else
std::cerr << "[V] no filename for md5 given, not creating hash" << std::endl;
if ( md5filename.size() )
{
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Tmd5cb(new ::libmaus::lz::BgzfDeflateOutputCallbackMD5);
Pmd5cb = UNIQUE_PTR_MOVE(Tmd5cb);
cbs.push_back(Pmd5cb.get());
}
}
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Pindex;
if ( arginfo.getValue<unsigned int>("index",getDefaultIndex()) )
{
if ( arginfo.hasArg("indexfilename") && arginfo.getUnparsedValue("indexfilename","") != "" )
indexfilename = arginfo.getUnparsedValue("indexfilename","");
else
std::cerr << "[V] no filename for index given, not creating index" << std::endl;
if ( indexfilename.size() )
{
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Tindex(new libmaus::bambam::BgzfDeflateOutputCallbackBamIndex(tmpfileindex));
Pindex = UNIQUE_PTR_MOVE(Tindex);
cbs.push_back(Pindex.get());
}
}
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > * Pcbs = 0;
if ( cbs.size() )
Pcbs = &cbs;
/*
* end md5/index callbacks
*/
::libmaus::bambam::BamWriter::unique_ptr_type writer(new ::libmaus::bambam::BamWriter(std::cout,uphead,level,Pcbs));
::libmaus::bambam::BamAlignment & algn = bamdec.getAlignment();
::libmaus::bambam::BamAlignment & prealgn = bampredec.getAlignment();
int64_t curid = -1;
libmaus::autoarray::AutoArray< std::pair<uint8_t,uint8_t> > auxpre;
libmaus::autoarray::AutoArray< std::pair<uint8_t,uint8_t> > auxnew;
libmaus::bambam::BamAuxFilterVector auxfilter;
// helpers for clipReinsert
libmaus::autoarray::AutoArray < std::pair<uint8_t,uint8_t> > auxtags;
libmaus::autoarray::AutoArray<libmaus::bambam::cigar_operation> cigop;
std::stack < libmaus::bambam::cigar_operation > hardstack;
libmaus::bambam::BamAlignment::D_array_type Tcigar;
libmaus::bambam::BamAuxFilterVector bafv;
libmaus::bambam::BamAuxFilterVector auxfilterout;
auxfilterout.set('q','s');
auxfilterout.set('q','q');
// helpers for zztoname
libmaus::bambam::BamAuxFilterVector zzbafv;
zzbafv.set('z','z');
// loop over aligned BAM file
while ( bamdec.readAlignment() )
{
if ( ranksplit )
split12(algn);
// extract rank
char const * name = algn.getName();
char const * u1 = name;
bool ok = true;
uint64_t rank = 0;
while ( *u1 && *u1 != '_' )
{
rank *= 10;
rank += (*u1-'0');
ok = ok && isdigit(*u1);
++u1;
}
// unable to find rank? write out as is and continue
if ( ! ok )
{
algn.serialise(writer->getStream());
continue;
}
// loop over unaligned BAM file
while ( curid != static_cast<int64_t>(rank) )
{
bool const a_ok = bampredec.readAlignment();
if ( ! a_ok )
{
libmaus::exception::LibMausException se;
se.getStream() << "Found unexpected EOF on file " << prefilename << std::endl;
se.finish();
throw se;
}
assert ( a_ok );
++curid;
if ( verbose && (! (curid & bmask)) )
std::cerr << "[V] " << (curid / bmod) << std::endl;
}
if ( verbose > 1 )
std::cerr << "Merging:\n" << algn.formatAlignment(header) << "\n" << prealgn.formatAlignment(preheader) << std::endl;
uint64_t pretagnum = prealgn.enumerateAuxTags(auxpre);
uint64_t newtagnum = algn.enumerateAuxTags(auxnew);
std::sort(auxpre.begin(),auxpre.begin()+pretagnum);
std::sort(auxnew.begin(),auxnew.begin()+newtagnum);
if ( verbose > 1 )
std::cerr << "pretagnum=" << pretagnum << " newtagnum=" << newtagnum << std::endl;
std::pair<uint8_t,uint8_t> * prec = auxpre.begin();
std::pair<uint8_t,uint8_t> * pree = prec + pretagnum;
std::pair<uint8_t,uint8_t> * preo = prec;
std::pair<uint8_t,uint8_t> * newc = auxnew.begin();
std::pair<uint8_t,uint8_t> * newe = newc + newtagnum;
std::pair<uint8_t,uint8_t> * newo = newc;
while ( prec != pree && newc != newe )
{
// pre which is not in new
if ( *prec < *newc )
{
*(preo++) = *(prec++);
}
// tag in both, drop pre
else if ( *prec == *newc )
{
*(newo++) = *(newc++);
prec++;
}
// new not in pre
else
{
*(newo++) = *(newc++);
}
}
while ( prec != pree )
*(preo++) = *(prec++);
while ( newc != newe )
*(newo++) = *(newc++);
pretagnum = preo-auxpre.begin();
newtagnum = newo-auxnew.begin();
for ( uint64_t i = 0; i < pretagnum; ++i )
auxfilter.set(auxpre[i].first,auxpre[i].second);
algn.copyAuxTags(prealgn, auxfilter);
for ( uint64_t i = 0; i < pretagnum; ++i )
auxfilter.clear(auxpre[i].first,auxpre[i].second);
if ( verbose > 1 )
{
std::cerr << "pretagnum=" << pretagnum << " newtagnum=" << newtagnum << std::endl;
std::cerr << "result: " << algn.formatAlignment(header) << std::endl;
}
// copy QC fail flag from original file to aligner output
if ( prealgn.isQCFail() )
algn.putFlags( algn.getFlags() | libmaus::bambam::BamFlagBase::LIBMAUS_BAMBAM_FQCFAIL );
if ( rankstrip )
strip12(algn);
if ( clipreinsert )
clipReinsert(algn,auxtags,bafv,cigop,Tcigar,hardstack,auxfilterout);
if ( zztoname )
zzToRank(algn,zzbafv);
algn.serialise(writer->getStream());
}
writer.reset();
if ( Pmd5cb )
{
Pmd5cb->saveDigestAsFile(md5filename);
}
if ( Pindex )
{
Pindex->flush(std::string(indexfilename));
}
return EXIT_SUCCESS;
}
int bamsort(::libmaus::util::ArgInfo const & arginfo)
{
::libmaus::util::TempFileRemovalContainer::setup();
bool const inputisstdin = (!arginfo.hasArg("I")) || (arginfo.getUnparsedValue("I","-") == "-");
bool const outputisstdout = (!arginfo.hasArg("O")) || (arginfo.getUnparsedValue("O","-") == "-");
if ( isatty(STDIN_FILENO) && inputisstdin && (arginfo.getValue<std::string>("inputformat","bam") != "sam") )
{
::libmaus::exception::LibMausException se;
se.getStream() << "Refusing to read binary data from terminal, please redirect standard input to pipe or file." << std::endl;
se.finish();
throw se;
}
if ( isatty(STDOUT_FILENO) && outputisstdout && (arginfo.getValue<std::string>("outputformat","bam") != "sam") )
{
::libmaus::exception::LibMausException se;
se.getStream() << "Refusing write binary data to terminal, please redirect standard output to pipe or file." << std::endl;
se.finish();
throw se;
}
int const verbose = arginfo.getValue<int>("verbose",getDefaultVerbose());
bool const disablevalidation = arginfo.getValue<int>("disablevalidation",getDefaultDisableValidation());
std::string const inputformat = arginfo.getUnparsedValue("inputformat",getDefaultInputFormat());
int const level = arginfo.getValue<int>("level",getDefaultLevel());
switch ( level )
{
case Z_NO_COMPRESSION:
case Z_BEST_SPEED:
case Z_BEST_COMPRESSION:
case Z_DEFAULT_COMPRESSION:
break;
default:
{
::libmaus::exception::LibMausException se;
se.getStream()
<< "Unknown compression level, please use"
<< " level=" << Z_DEFAULT_COMPRESSION << " (default) or"
<< " level=" << Z_BEST_SPEED << " (fast) or"
<< " level=" << Z_BEST_COMPRESSION << " (best) or"
<< " level=" << Z_NO_COMPRESSION << " (no compression)" << std::endl;
se.finish();
throw se;
}
break;
}
// prefix for tmp files
std::string const tmpfilenamebase = arginfo.getValue<std::string>("tmpfile",arginfo.getDefaultTmpFileName());
std::string const tmpfilenameout = tmpfilenamebase + "_bamsort";
::libmaus::util::TempFileRemovalContainer::addTempFile(tmpfilenameout);
uint64_t blockmem = arginfo.getValue<uint64_t>("blockmb",getDefaultBlockSize())*1024*1024;
std::string const sortorder = arginfo.getValue<std::string>("SO","coordinate");
bool const fixmates = arginfo.getValue<int>("fixmates",getDefaultFixMates());
uint64_t sortthreads = arginfo.getValue<uint64_t>("sortthreads",getDefaultSortThreads());
// input decoder wrapper
libmaus::bambam::BamAlignmentDecoderWrapper::unique_ptr_type decwrapper(
libmaus::bambam::BamMultiAlignmentDecoderFactory::construct(
arginfo,false // put rank
)
);
::libmaus::bambam::BamAlignmentDecoder * ppdec = &(decwrapper->getDecoder());
::libmaus::bambam::BamAlignmentDecoder & dec = *ppdec;
if ( disablevalidation )
dec.disableValidation();
::libmaus::bambam::BamHeader const & header = dec.getHeader();
std::string const headertext(header.text);
// add PG line to header
std::string const upheadtext = ::libmaus::bambam::ProgramHeaderLineSet::addProgramLine(
headertext,
"bamsort", // ID
"bamsort", // PN
arginfo.commandline, // CL
::libmaus::bambam::ProgramHeaderLineSet(headertext).getLastIdInChain(), // PP
std::string(PACKAGE_VERSION) // VN
);
// construct new header
::libmaus::bambam::BamHeader uphead(upheadtext);
/*
* start index/md5 callbacks
*/
std::string const tmpfileindex = tmpfilenamebase + "_index";
::libmaus::util::TempFileRemovalContainer::addTempFile(tmpfileindex);
std::string md5filename;
std::string indexfilename;
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > cbs;
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Pmd5cb;
if ( arginfo.getValue<unsigned int>("md5",getDefaultMD5()) )
{
if ( arginfo.hasArg("md5filename") && arginfo.getUnparsedValue("md5filename","") != "" )
md5filename = arginfo.getUnparsedValue("md5filename","");
else
std::cerr << "[V] no filename for md5 given, not creating hash" << std::endl;
if ( md5filename.size() )
{
::libmaus::lz::BgzfDeflateOutputCallbackMD5::unique_ptr_type Tmd5cb(new ::libmaus::lz::BgzfDeflateOutputCallbackMD5);
Pmd5cb = UNIQUE_PTR_MOVE(Tmd5cb);
cbs.push_back(Pmd5cb.get());
}
}
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Pindex;
if ( arginfo.getValue<unsigned int>("index",getDefaultIndex()) )
{
if ( arginfo.hasArg("indexfilename") && arginfo.getUnparsedValue("indexfilename","") != "" )
indexfilename = arginfo.getUnparsedValue("indexfilename","");
else
std::cerr << "[V] no filename for index given, not creating index" << std::endl;
if ( indexfilename.size() )
{
libmaus::bambam::BgzfDeflateOutputCallbackBamIndex::unique_ptr_type Tindex(new libmaus::bambam::BgzfDeflateOutputCallbackBamIndex(tmpfileindex));
Pindex = UNIQUE_PTR_MOVE(Tindex);
cbs.push_back(Pindex.get());
}
}
std::vector< ::libmaus::lz::BgzfDeflateOutputCallback * > * Pcbs = 0;
if ( cbs.size() )
Pcbs = &cbs;
/*
* end md5/index callbacks
*/
if ( sortorder != "queryname" )
uphead.changeSortOrder("coordinate");
else
uphead.changeSortOrder("queryname");
libmaus::bambam::BamBlockWriterBase::unique_ptr_type Pout ( libmaus::bambam::BamBlockWriterBaseFactory::construct(uphead, arginfo, Pcbs) );
if ( fixmates )
{
if ( sortorder != "queryname" )
{
::libmaus::bambam::BamEntryContainer< ::libmaus::bambam::BamAlignmentPosComparator >
BEC(blockmem,tmpfilenameout,sortthreads);
if ( verbose )
std::cerr << "[V] Reading alignments from source." << std::endl;
uint64_t incnt = 0;
// current alignment
libmaus::bambam::BamAlignment & curalgn = dec.getAlignment();
// previous alignment
libmaus::bambam::BamAlignment prevalgn;
// previous alignment valid
bool prevalgnvalid = false;
// MQ field filter
libmaus::bambam::BamAuxFilterVector MQfilter;
MQfilter.set("MQ");
while ( dec.readAlignment() )
{
if ( curalgn.isSecondary() || curalgn.isSupplementary() )
{
BEC.putAlignment(curalgn);
}
else if ( prevalgnvalid )
{
// different name
if ( strcmp(curalgn.getName(),prevalgn.getName()) )
{
BEC.putAlignment(prevalgn);
curalgn.swap(prevalgn);
}
// same name
else
{
libmaus::bambam::BamAlignment::fixMateInformation(prevalgn,curalgn,MQfilter);
BEC.putAlignment(prevalgn);
BEC.putAlignment(curalgn);
prevalgnvalid = false;
}
}
else
{
prevalgn.swap(curalgn);
prevalgnvalid = true;
}
if ( verbose && ( ( ++incnt & ((1ull<<20)-1) ) == 0 ) )
std::cerr << "[V] " << incnt << std::endl;
}
if ( prevalgnvalid )
{
BEC.putAlignment(prevalgn);
prevalgnvalid = false;
}
if ( verbose )
std::cerr << "[V] read " << incnt << " alignments" << std::endl;
// BEC.createOutput(std::cout, uphead, level, verbose, Pcbs);
BEC.createOutput(*Pout, verbose);
}
else
{
::libmaus::bambam::BamEntryContainer< ::libmaus::bambam::BamAlignmentNameComparator >
BEC(blockmem,tmpfilenameout,sortthreads);
if ( verbose )
std::cerr << "[V] Reading alignments from source." << std::endl;
uint64_t incnt = 0;
// current alignment
libmaus::bambam::BamAlignment & curalgn = dec.getAlignment();
// previous alignment
libmaus::bambam::BamAlignment prevalgn;
// previous alignment valid
bool prevalgnvalid = false;
// MQ field filter
libmaus::bambam::BamAuxFilterVector MQfilter;
MQfilter.set("MQ");
while ( dec.readAlignment() )
{
if ( curalgn.isSecondary() || curalgn.isSupplementary() )
{
BEC.putAlignment(curalgn);
}
else if ( prevalgnvalid )
{
// different name
if ( strcmp(curalgn.getName(),prevalgn.getName()) )
{
BEC.putAlignment(prevalgn);
curalgn.swap(prevalgn);
}
// same name
else
{
libmaus::bambam::BamAlignment::fixMateInformation(prevalgn,curalgn,MQfilter);
BEC.putAlignment(prevalgn);
BEC.putAlignment(curalgn);
prevalgnvalid = false;
}
}
else
{
prevalgn.swap(curalgn);
prevalgnvalid = true;
}
if ( verbose && ( ( ++incnt & ((1ull<<20)-1) ) == 0 ) )
std::cerr << "[V] " << incnt << std::endl;
}
if ( prevalgnvalid )
{
BEC.putAlignment(prevalgn);
prevalgnvalid = false;
}
if ( verbose )
std::cerr << "[V] read " << incnt << " alignments" << std::endl;
// BEC.createOutput(std::cout, uphead, level, verbose, Pcbs);
BEC.createOutput(*Pout, verbose);
}
}
else
{
if ( sortorder != "queryname" )
{
::libmaus::bambam::BamEntryContainer< ::libmaus::bambam::BamAlignmentPosComparator > BEC(blockmem,tmpfilenameout,sortthreads);
if ( verbose )
std::cerr << "[V] Reading alignments from source." << std::endl;
uint64_t incnt = 0;
while ( dec.readAlignment() )
{
BEC.putAlignment(dec.getAlignment());
incnt++;
if ( verbose && (incnt % (1024*1024) == 0) )
std::cerr << "[V] " << incnt/(1024*1024) << "M" << std::endl;
}
if ( verbose )
std::cerr << "[V] read " << incnt << " alignments" << std::endl;
// BEC.createOutput(std::cout, uphead, level, verbose, Pcbs);
BEC.createOutput(*Pout, verbose);
}
else
{
::libmaus::bambam::BamEntryContainer< ::libmaus::bambam::BamAlignmentNameComparator > BEC(blockmem,tmpfilenameout,sortthreads);
if ( verbose )
std::cerr << "[V] Reading alignments from source." << std::endl;
uint64_t incnt = 0;
while ( dec.readAlignment() )
{
BEC.putAlignment(dec.getAlignment());
incnt++;
if ( verbose && (incnt % (1024*1024) == 0) )
std::cerr << "[V] " << incnt/(1024*1024) << "M" << std::endl;
}
if ( verbose )
std::cerr << "[V] read " << incnt << " alignments" << std::endl;
// BEC.createOutput(std::cout, uphead, level, verbose, Pcbs);
BEC.createOutput(*Pout, verbose);
}
}
// flush encoder so callbacks see all output data
Pout.reset();
if ( Pmd5cb )
{
Pmd5cb->saveDigestAsFile(md5filename);
}
if ( Pindex )
{
Pindex->flush(std::string(indexfilename));
}
return EXIT_SUCCESS;
}